sqa cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sqa
Entrez Gene ID	36002
Full Name	spaghetti-squash activator
Synonyms	CG1776,CG30004,CG42347,Dmel\CG42347,Dmel_CG1776,Dmel_CG30004,FBgn0259678
General protein information	Preferred Names spaghetti-squash activator Names CG42347 gene product from transcript CG42347-RD CG42347-PB CG42347-PC CG42347-PD MLCK-like sqa-PB sqa-PC sqa-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 46B1-46B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273903.2	NP_001260832.1	spaghetti-squash activator, isoform D
NM_136670.3	NP_610514.1	spaghetti-squash activator, isoform B
NM_165693.4	NP_724821.2	spaghetti-squash activator, isoform C

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MYLK2	XP_005634973.1
Drosophila melanogaster (fruit fly)	sqa	NP_610514.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006746	XP_308996.3
Bos taurus (cattle)	MYLK2	NP_001077188.1
Rattus norvegicus (Norway rat)	Mylk2	NP_476557.1
	LOC712830	XP_002798235.1
Gallus gallus (chicken)	MYLK2	NP_990723.1
Homo sapiens (human)	MYLK2	NP_149109.1
Pan troglodytes (chimpanzee)	MYLK2	XP_003316951.1
Mus musculus (house mouse)	Mylk2	NP_001074513.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sqa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sqa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa12345	NM_136670.3 Latest version!	Drosophila melanogaster spaghetti-squash activator (sqa), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09396	NM_165693.4 Latest version!	Drosophila melanogaster spaghetti-squash activator (sqa), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa09396	NM_001273903.2 Latest version!	Drosophila melanogaster spaghetti-squash activator (sqa), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sqa ( NM_136670.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610514.1 Drosophila melanogaster spaghetti-squash activator (sqa), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12345
Clone ID Related Accession (Same CDS sequence)	NM_136670.3
Accession Version	NM_136670.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spaghetti-squash activator, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136670.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCTATA TAGATGACTC AGAGCCGGAA GGTGTTTTGG AACCGGCGTT TCCCATGCGC 
GATGTGACCA TAAATCGCAA CGTCGATGCC CATAAACATT ACGATGTGCT TGGTGAGGTG 
GGACGCGGAA AATTCGGTAC GGTGTACAAG TGCAGGGACA AGGCGAATGG ACTGCAGCTG 
GCGGCCAAGT TTGTGCCGAT CCCCAAGCGC GAGGACAAGC GGAATGTGGA GCGGGAGGTG 
GAGATAATGA ATTCATTGCA GCATCACCTC ATCATACAGT TGTACGCGGC ATACGAATAT 
CAGAAAATGA TGTGCGTCGT CCTGGAACTC ATCGAAGGAG GTGAGCTCTT TGATCGCGTG 
GTGGACGATG AGTTCGTCCT CACCGAACGA GTCTGCCGCG TCTTCATCCG CCAGGTGTGC 
GAGGCCATGG CCTTCATCCA CGGCAATGGA ATAGTCCACC TCGATCTGAA GCCCGAAAAC 
ATTTTGGTGC TCACACAGAA GGGCAACCGC ATCAAGATCA TTGACTTCGG ACTGGCTCGT 
AAATTTGATC CTGATAAGCG ACTCAGAGTT CTCTTCGGCA CTCCGGAATT TGTGGCGCCA 
GAAGTTGTGA ACTTTGACTG TATATCGTAT GGAACAGATA TGTGGAGTGT TGGCGTTATA 
TGCTATGTGC TCATCTCAGG ACTGTCGCCT TTCATGGGCG AGAACGACAT TGAAACAATG 
TCAAATGTAA CCATTGCAAA ATACGACTTC GAGGATGAAT GCTTTAATGG CATATCCCCC 
GAGTGTCTGG ATTTCATAGC CAAGCTGCTG GCCAAGGACC TGAGCACCCG GATGACAGCC 
GCCGAGTGCA TGAAGCACAA ATGGCTTCAG CAGCGGCCGG CCACCGCCGC CACGGCCACG 
CCCATCACAA AGGCCGCCTC CGCGGCGTCG AAGTCGCGCC TGAAGTCTGT GTCTCCGGTG 
ACAGCTCCCT CGGAATCCTC CGAGGACTCC ACGGAAACCA TTGAGGACGA AGACGACGAG 
GAAGAGGTTG CCGTGCAGCA GGCGAAGCAG AAAGACCAGC AGCAGGACGA GGAGCTGGCC 
AATCTCTGCG GCGACGCTGA ACTTGAGAAC AAAGAACTGG ATGCCACGAA GGACAACCTT 
AAGAACTTCA TCGTGCGCTG GGAGACGCAT CCGAACAGTC CGTACGTGTT CGACGTGGAG 
GGGAATGTGA TTGCTCCGCT GAGCGAGACG AGCTACCCCC ATCCCAGGAG GACCCACGGC 
GCGGACAGCC TTTCCTCCTC CAGAGGTGAG TACGGATCCC AGCGACAGCG CGACCGACAC 
GCCCTCATCC ACGGCAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610514.1
CDS	377..1717
Translation	MIYIDDSEPEGVLEPAFPMRDVTINRNVDAHKHYDVLGEVGRGKFGTVYKCRDKANGLQLAAKFVPIPKREDKRNVEREVEIMNSLQHHLIIQLYAAYEYQKMMCVVLELIEGGELFDRVVDDEFVLTERVCRVFIRQVCEAMAFIHGNGIVHLDLKPENILVLTQKGNRIKIIDFGLARKFDPDKRLRVLFGTPEFVAPEVVNFDCISYGTDMWSVGVICYVLISGLSPFMGENDIETMSNVTIAKYDFEDECFNGISPECLDFIAKLLAKDLSTRMTAAECMKHKWLQQRPATAATATPITKAASAASKSRLKSVSPVTAPSESSEDSTETIEDEDDEEEVAVQQAKQKDQQQDEELANLCGDAELENKELDATKDNLKNFIVRWETHPNSPYVFDVEGNVIAPLSETSYPHPRRTHGADSLSSSRGEYGSQRQRDRHALIHGK

Target ORF information:

RefSeq Version	NM_136670.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spaghetti-squash activator (sqa), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136670.3

ORF Insert Sequence:

ATGATCTATA TAGATGACTC AGAGCCGGAA GGTGTTTTGG AACCGGCGTT TCCCATGCGC 
GATGTGACCA TAAATCGCAA CGTCGATGCC CATAAACATT ACGATGTGCT TGGTGAGGTG 
GGACGCGGAA AATTCGGTAC GGTGTACAAG TGCAGGGACA AGGCGAATGG ACTGCAGCTG 
GCGGCCAAGT TTGTGCCGAT CCCCAAGCGC GAGGACAAGC GGAATGTGGA GCGGGAGGTG 
GAGATAATGA ATTCATTGCA GCATCACCTC ATCATACAGT TGTACGCGGC ATACGAATAT 
CAGAAAATGA TGTGCGTCGT CCTGGAACTC ATCGAAGGAG GTGAGCTCTT TGATCGCGTG 
GTGGACGATG AGTTCGTCCT CACCGAACGA GTCTGCCGCG TCTTCATCCG CCAGGTGTGC 
GAGGCCATGG CCTTCATCCA CGGCAATGGA ATAGTCCACC TCGATCTGAA GCCCGAAAAC 
ATTTTGGTGC TCACACAGAA GGGCAACCGC ATCAAGATCA TTGACTTCGG ACTGGCTCGT 
AAATTTGATC CTGATAAGCG ACTCAGAGTT CTCTTCGGCA CTCCGGAATT TGTGGCGCCA 
GAAGTTGTGA ACTTTGACTG TATATCGTAT GGAACAGATA TGTGGAGTGT TGGCGTTATA 
TGCTATGTGC TCATCTCAGG ACTGTCGCCT TTCATGGGCG AGAACGACAT TGAAACAATG 
TCAAATGTAA CCATTGCAAA ATACGACTTC GAGGATGAAT GCTTTAATGG CATATCCCCC 
GAGTGTCTGG ATTTCATAGC CAAGCTGCTG GCCAAGGACC TGAGCACCCG GATGACAGCC 
GCCGAGTGCA TGAAGCACAA ATGGCTTCAG CAGCGGCCGG CCACCGCCGC CACGGCCACG 
CCCATCACAA AGGCCGCCTC CGCGGCGTCG AAGTCGCGCC TGAAGTCTGT GTCTCCGGTG 
ACAGCTCCCT CGGAATCCTC CGAGGACTCC ACGGAAACCA TTGAGGACGA AGACGACGAG 
GAAGAGGTTG CCGTGCAGCA GGCGAAGCAG AAAGACCAGC AGCAGGACGA GGAGCTGGCC 
AATCTCTGCG GCGACGCTGA ACTTGAGAAC AAAGAACTGG ATGCCACGAA GGACAACCTT 
AAGAACTTCA TCGTGCGCTG GGAGACGCAT CCGAACAGTC CGTACGTGTT CGACGTGGAG 
GGGAATGTGA TTGCTCCGCT GAGCGAGACG AGCTACCCCC ATCCCAGGAG GACCCACGGC 
GCGGACAGCC TTTCCTCCTC CAGAGGTGAG TACGGATCCC AGCGACAGCG CGACCGACAC 
GCCCTCATCC ACGGCAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sqa ( NM_001273903.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260832.1 Drosophila melanogaster spaghetti-squash activator (sqa), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa09396
Clone ID Related Accession (Same CDS sequence)	NM_001273903.2 , NM_165693.4
Accession Version	NM_001273903.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spaghetti-squash activator, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001273903.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCTATA TAGATGACTC AGAGCCGGAA GGTGTTTTGG AACCGGCGTT TCCCATGCGC 
GATGTGACCA TAAATCGCAA CGTCGATGCC CATAAACATT ACGATGTGCT TGGTGAGGTG 
GGACGCGGAA AATTCGGTAC GGTGTACAAG TGCAGGGACA AGGCGAATGG ACTGCAGCTG 
GCGGCCAAGT TTGTGCCGAT CCCCAAGCGC GAGGACAAGC GGAATGTGGA GCGGGAGGTG 
GAGATAATGA ATTCATTGCA GCATCACCTC ATCATACAGT TGTACGCGGC ATACGAATAT 
CAGAAAATGA TGTGCGTCGT CCTGGAACTC ATCGAAGGAG GTGAGCTCTT TGATCGCGTG 
GTGGACGATG AGTTCGTCCT CACCGAACGA GTCTGCCGCG TCTTCATCCG CCAGGTGTGC 
GAGGCCATGG CCTTCATCCA CGGCAATGGA ATAGTCCACC TCGATCTGAA GCCCGAAAAC 
ATTTTGGTGC TCACACAGAA GGGCAACCGC ATCAAGATCA TTGACTTCGG ACTGGCTCGT 
AAATTTGATC CTGATAAGCG ACTCAGAGTT CTCTTCGGCA CTCCGGAATT TGTGGCGCCA 
GAAGTTGTGA ACTTTGACTG TATATCGTAT GGAACAGATA TGTGGAGTGT TGGCGTTATA 
TGCTATGTGC TCATCTCAGG ACTGTCGCCT TTCATGGGCG AGAACGACAT TGAAACAATG 
TCAAATGTAA CCATTGCAAA ATACGACTTC GAGGATGAAT GCTTTAATGG CATATCCCCC 
GAGTGTCTGG ATTTCATAGC CAAGCTGCTG GCCAAGGACC TGAGCACCCG GATGACAGCC 
GCCGAGTGCA TGAAGCACAA ATGGCTTCAG CAGCGGCCGG CCACCGCCGC CACGGCCACG 
CCCATCACAA AGGCCGCCTC CGCGGCGTCG AAGTCGCGCC TGAAGTCTGT GTCTCCGGTG 
ACAGCTCCCT CGGAATCCTC CGAGGACTCC ACGGAAACCA TTGAGGACGA AGACGACGAG 
GAAGAGGTTG CCGTGCAGCA GGCGAAGCAG AAAGACCAGC AGCAGGACGA GGAGCTGGCC 
AATCTCTGCG GCGACGCTGA ACTTGAGAAC AAAGAACTGG ATGCCACGAA GGACAACCTT 
AAGAACTTCA TCGTGCGCTG GGAGACGCAT CCGAACAGTC CGTACGTGTT CGACGTGGAG 
GGGAATGTGA TTGCTCCGCT GAGCGAGACG AGCTACCCCC ATCCCAGGAG GACCCACGGC 
GCGGACAGCC TTTCCTCCTC CAGAGTTTGT TCGCCTTCGC CCTGCGATTC AATATCCACT 
TTGACGGATG ACGAACGGGG AGATATTGAA GATCTTCCGG AAGAGGACGA ATCCCGCAGT 
GCGGAAAACG AGAGTCCTCG CTCGGTGGCC ACGCCCATCA ACGAGTCTCG GGAGAAGCTG 
TTCCCGACGG TAGCCACCTC CAGTCCGTCA ACTCCCACGC CGCAGCATCT CTTCAACGAG 
AACTTCGATG AGTTCTCTGG CAGCCAGTCC ACCGCCCAGC AGCAGCGCAG CATGAAGAGC 
TATCTGCACA CCTTCGATCG AAGGAACTCG GATACCACGT ACCTGCTGGG TCGGCGGAGT 
TCCGGAGAGC GGGTGAATCT GGCGGATGAG ATACGAAAGC TATCCGATCA CCTGCTCATG 
CTGGCCGAGA TCAACACCAA GCTTGGGGAT GCGAACAATA ACGGGAGCAG CAGTGGAGCG 
CCTGCTGATC CTCCTGCTGC TGCTGCTGCT GCTGCTGCTC CTGTGCCAAC CTCAACTGCT 
CCAGCATCTG TTGCTCCTTC TGGCAGTACT TCTAGTAAAA CCTCGGAGAT CAGCCAGGAA 
GGCAATAGGT GGACCAGCAA AACCACTTCG AGCAGTAGCT GGAATCGTCC CACTTTGAAA 
GGAGGACTCT TCAGCCAGGC CAGCAGCAGT TCCCAGAGCC AAAGCACCTA CGATGACGGA 
AAGGGCAAGA CGAAGATCAG TTCGCTGTCT GTGAGATTGC AGCAGTCCAT CGAGGAAACA 
CCCAAGCTAA GCAACGGAAA TAGCTCCTCT AGCAAATCCC TGGTCCAGTC GCAGCGAAAG 
TCCACGGTGC AAACCATGAG CAGTTCCAAT GCCAGGAGCT TCACCAACCA GCAAGTACAA 
AGAACCAGCA CCACCTCTTC CAAGGTGATC TCCAGCAGCA CCAGAAGCAG TAACACCAGC 
TCCAGCAATT ATATAGCCTC CAACGCCAGC AACTCAATCT CGACCAGCGC CAGCAACCCA 
AGCTCCACCG GCGCCAGCAA TCCAATTACC ACCAGCGCCA GCAACGAATC GGCCAGCAGT 
CGTCGAGCCA AGTTCAGGAT AAATCAGATG AGTCGCGATG TGCCCGTTGG GCTGCCGGAC 
ACTCATCAAA CCGTGAATCT GGAGGAGGCG GCCAACACCA CCAAGGATTG CCTGCTGCAT 
TTGTTGGAGA AATACAACGA GACCCGCATC CGCAATCCGG TGGGTCGTCA CCAGAGCATC 
AGCGTGGACT GGCGGGTCAG CGACAATCTG GAGTACCGCT CCATGAGCTC CATCAACGCC 
TTCTTCCAGC GCCACAACCC CAATGGCGGC GGCCACAACG TACGCCACAT TCAAGCCCAG 
CTGGAGGAGA AGGCGGCGGG CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260832.1
CDS	3225..5891
Translation	MIYIDDSEPEGVLEPAFPMRDVTINRNVDAHKHYDVLGEVGRGKFGTVYKCRDKANGLQLAAKFVPIPKREDKRNVEREVEIMNSLQHHLIIQLYAAYEYQKMMCVVLELIEGGELFDRVVDDEFVLTERVCRVFIRQVCEAMAFIHGNGIVHLDLKPENILVLTQKGNRIKIIDFGLARKFDPDKRLRVLFGTPEFVAPEVVNFDCISYGTDMWSVGVICYVLISGLSPFMGENDIETMSNVTIAKYDFEDECFNGISPECLDFIAKLLAKDLSTRMTAAECMKHKWLQQRPATAATATPITKAASAASKSRLKSVSPVTAPSESSEDSTETIEDEDDEEEVAVQQAKQKDQQQDEELANLCGDAELENKELDATKDNLKNFIVRWETHPNSPYVFDVEGNVIAPLSETSYPHPRRTHGADSLSSSRVCSPSPCDSISTLTDDERGDIEDLPEEDESRSAENESPRSVATPINESREKLFPTVATSSPSTPTPQHLFNENFDEFSGSQSTAQQQRSMKSYLHTFDRRNSDTTYLLGRRSSGERVNLADEIRKLSDHLLMLAEINTKLGDANNNGSSSGAPADPPAAAAAAAAPVPTSTAPASVAPSGSTSSKTSEISQEGNRWTSKTTSSSSWNRPTLKGGLFSQASSSSQSQSTYDDGKGKTKISSLSVRLQQSIEETPKLSNGNSSSSKSLVQSQRKSTVQTMSSSNARSFTNQQVQRTSTTSSKVISSSTRSSNTSSSNYIASNASNSISTSASNPSSTGASNPITTSASNESASSRRAKFRINQMSRDVPVGLPDTHQTVNLEEAANTTKDCLLHLLEKYNETRIRNPVGRHQSISVDWRVSDNLEYRSMSSINAFFQRHNPNGGGHNVRHIQAQLEEKAAGK

Target ORF information:

RefSeq Version	NM_001273903.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spaghetti-squash activator (sqa), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273903.2

ORF Insert Sequence:

ATGATCTATA TAGATGACTC AGAGCCGGAA GGTGTTTTGG AACCGGCGTT TCCCATGCGC 
GATGTGACCA TAAATCGCAA CGTCGATGCC CATAAACATT ACGATGTGCT TGGTGAGGTG 
GGACGCGGAA AATTCGGTAC GGTGTACAAG TGCAGGGACA AGGCGAATGG ACTGCAGCTG 
GCGGCCAAGT TTGTGCCGAT CCCCAAGCGC GAGGACAAGC GGAATGTGGA GCGGGAGGTG 
GAGATAATGA ATTCATTGCA GCATCACCTC ATCATACAGT TGTACGCGGC ATACGAATAT 
CAGAAAATGA TGTGCGTCGT CCTGGAACTC ATCGAAGGAG GTGAGCTCTT TGATCGCGTG 
GTGGACGATG AGTTCGTCCT CACCGAACGA GTCTGCCGCG TCTTCATCCG CCAGGTGTGC 
GAGGCCATGG CCTTCATCCA CGGCAATGGA ATAGTCCACC TCGATCTGAA GCCCGAAAAC 
ATTTTGGTGC TCACACAGAA GGGCAACCGC ATCAAGATCA TTGACTTCGG ACTGGCTCGT 
AAATTTGATC CTGATAAGCG ACTCAGAGTT CTCTTCGGCA CTCCGGAATT TGTGGCGCCA 
GAAGTTGTGA ACTTTGACTG TATATCGTAT GGAACAGATA TGTGGAGTGT TGGCGTTATA 
TGCTATGTGC TCATCTCAGG ACTGTCGCCT TTCATGGGCG AGAACGACAT TGAAACAATG 
TCAAATGTAA CCATTGCAAA ATACGACTTC GAGGATGAAT GCTTTAATGG CATATCCCCC 
GAGTGTCTGG ATTTCATAGC CAAGCTGCTG GCCAAGGACC TGAGCACCCG GATGACAGCC 
GCCGAGTGCA TGAAGCACAA ATGGCTTCAG CAGCGGCCGG CCACCGCCGC CACGGCCACG 
CCCATCACAA AGGCCGCCTC CGCGGCGTCG AAGTCGCGCC TGAAGTCTGT GTCTCCGGTG 
ACAGCTCCCT CGGAATCCTC CGAGGACTCC ACGGAAACCA TTGAGGACGA AGACGACGAG 
GAAGAGGTTG CCGTGCAGCA GGCGAAGCAG AAAGACCAGC AGCAGGACGA GGAGCTGGCC 
AATCTCTGCG GCGACGCTGA ACTTGAGAAC AAAGAACTGG ATGCCACGAA GGACAACCTT 
AAGAACTTCA TCGTGCGCTG GGAGACGCAT CCGAACAGTC CGTACGTGTT CGACGTGGAG 
GGGAATGTGA TTGCTCCGCT GAGCGAGACG AGCTACCCCC ATCCCAGGAG GACCCACGGC 
GCGGACAGCC TTTCCTCCTC CAGAGTTTGT TCGCCTTCGC CCTGCGATTC AATATCCACT 
TTGACGGATG ACGAACGGGG AGATATTGAA GATCTTCCGG AAGAGGACGA ATCCCGCAGT 
GCGGAAAACG AGAGTCCTCG CTCGGTGGCC ACGCCCATCA ACGAGTCTCG GGAGAAGCTG 
TTCCCGACGG TAGCCACCTC CAGTCCGTCA ACTCCCACGC CGCAGCATCT CTTCAACGAG 
AACTTCGATG AGTTCTCTGG CAGCCAGTCC ACCGCCCAGC AGCAGCGCAG CATGAAGAGC 
TATCTGCACA CCTTCGATCG AAGGAACTCG GATACCACGT ACCTGCTGGG TCGGCGGAGT 
TCCGGAGAGC GGGTGAATCT GGCGGATGAG ATACGAAAGC TATCCGATCA CCTGCTCATG 
CTGGCCGAGA TCAACACCAA GCTTGGGGAT GCGAACAATA ACGGGAGCAG CAGTGGAGCG 
CCTGCTGATC CTCCTGCTGC TGCTGCTGCT GCTGCTGCTC CTGTGCCAAC CTCAACTGCT 
CCAGCATCTG TTGCTCCTTC TGGCAGTACT TCTAGTAAAA CCTCGGAGAT CAGCCAGGAA 
GGCAATAGGT GGACCAGCAA AACCACTTCG AGCAGTAGCT GGAATCGTCC CACTTTGAAA 
GGAGGACTCT TCAGCCAGGC CAGCAGCAGT TCCCAGAGCC AAAGCACCTA CGATGACGGA 
AAGGGCAAGA CGAAGATCAG TTCGCTGTCT GTGAGATTGC AGCAGTCCAT CGAGGAAACA 
CCCAAGCTAA GCAACGGAAA TAGCTCCTCT AGCAAATCCC TGGTCCAGTC GCAGCGAAAG 
TCCACGGTGC AAACCATGAG CAGTTCCAAT GCCAGGAGCT TCACCAACCA GCAAGTACAA 
AGAACCAGCA CCACCTCTTC CAAGGTGATC TCCAGCAGCA CCAGAAGCAG TAACACCAGC 
TCCAGCAATT ATATAGCCTC CAACGCCAGC AACTCAATCT CGACCAGCGC CAGCAACCCA 
AGCTCCACCG GCGCCAGCAA TCCAATTACC ACCAGCGCCA GCAACGAATC GGCCAGCAGT 
CGTCGAGCCA AGTTCAGGAT AAATCAGATG AGTCGCGATG TGCCCGTTGG GCTGCCGGAC 
ACTCATCAAA CCGTGAATCT GGAGGAGGCG GCCAACACCA CCAAGGATTG CCTGCTGCAT 
TTGTTGGAGA AATACAACGA GACCCGCATC CGCAATCCGG TGGGTCGTCA CCAGAGCATC 
AGCGTGGACT GGCGGGTCAG CGACAATCTG GAGTACCGCT CCATGAGCTC CATCAACGCC 
TTCTTCCAGC GCCACAACCC CAATGGCGGC GGCCACAACG TACGCCACAT TCAAGCCCAG 
CTGGAGGAGA AGGCGGCGGG CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sqa ( NM_165693.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724821.2 Drosophila melanogaster spaghetti-squash activator (sqa), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa09396
Clone ID Related Accession (Same CDS sequence)	NM_001273903.2 , NM_165693.4
Accession Version	NM_165693.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spaghetti-squash activator, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165693.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCTATA TAGATGACTC AGAGCCGGAA GGTGTTTTGG AACCGGCGTT TCCCATGCGC 
GATGTGACCA TAAATCGCAA CGTCGATGCC CATAAACATT ACGATGTGCT TGGTGAGGTG 
GGACGCGGAA AATTCGGTAC GGTGTACAAG TGCAGGGACA AGGCGAATGG ACTGCAGCTG 
GCGGCCAAGT TTGTGCCGAT CCCCAAGCGC GAGGACAAGC GGAATGTGGA GCGGGAGGTG 
GAGATAATGA ATTCATTGCA GCATCACCTC ATCATACAGT TGTACGCGGC ATACGAATAT 
CAGAAAATGA TGTGCGTCGT CCTGGAACTC ATCGAAGGAG GTGAGCTCTT TGATCGCGTG 
GTGGACGATG AGTTCGTCCT CACCGAACGA GTCTGCCGCG TCTTCATCCG CCAGGTGTGC 
GAGGCCATGG CCTTCATCCA CGGCAATGGA ATAGTCCACC TCGATCTGAA GCCCGAAAAC 
ATTTTGGTGC TCACACAGAA GGGCAACCGC ATCAAGATCA TTGACTTCGG ACTGGCTCGT 
AAATTTGATC CTGATAAGCG ACTCAGAGTT CTCTTCGGCA CTCCGGAATT TGTGGCGCCA 
GAAGTTGTGA ACTTTGACTG TATATCGTAT GGAACAGATA TGTGGAGTGT TGGCGTTATA 
TGCTATGTGC TCATCTCAGG ACTGTCGCCT TTCATGGGCG AGAACGACAT TGAAACAATG 
TCAAATGTAA CCATTGCAAA ATACGACTTC GAGGATGAAT GCTTTAATGG CATATCCCCC 
GAGTGTCTGG ATTTCATAGC CAAGCTGCTG GCCAAGGACC TGAGCACCCG GATGACAGCC 
GCCGAGTGCA TGAAGCACAA ATGGCTTCAG CAGCGGCCGG CCACCGCCGC CACGGCCACG 
CCCATCACAA AGGCCGCCTC CGCGGCGTCG AAGTCGCGCC TGAAGTCTGT GTCTCCGGTG 
ACAGCTCCCT CGGAATCCTC CGAGGACTCC ACGGAAACCA TTGAGGACGA AGACGACGAG 
GAAGAGGTTG CCGTGCAGCA GGCGAAGCAG AAAGACCAGC AGCAGGACGA GGAGCTGGCC 
AATCTCTGCG GCGACGCTGA ACTTGAGAAC AAAGAACTGG ATGCCACGAA GGACAACCTT 
AAGAACTTCA TCGTGCGCTG GGAGACGCAT CCGAACAGTC CGTACGTGTT CGACGTGGAG 
GGGAATGTGA TTGCTCCGCT GAGCGAGACG AGCTACCCCC ATCCCAGGAG GACCCACGGC 
GCGGACAGCC TTTCCTCCTC CAGAGTTTGT TCGCCTTCGC CCTGCGATTC AATATCCACT 
TTGACGGATG ACGAACGGGG AGATATTGAA GATCTTCCGG AAGAGGACGA ATCCCGCAGT 
GCGGAAAACG AGAGTCCTCG CTCGGTGGCC ACGCCCATCA ACGAGTCTCG GGAGAAGCTG 
TTCCCGACGG TAGCCACCTC CAGTCCGTCA ACTCCCACGC CGCAGCATCT CTTCAACGAG 
AACTTCGATG AGTTCTCTGG CAGCCAGTCC ACCGCCCAGC AGCAGCGCAG CATGAAGAGC 
TATCTGCACA CCTTCGATCG AAGGAACTCG GATACCACGT ACCTGCTGGG TCGGCGGAGT 
TCCGGAGAGC GGGTGAATCT GGCGGATGAG ATACGAAAGC TATCCGATCA CCTGCTCATG 
CTGGCCGAGA TCAACACCAA GCTTGGGGAT GCGAACAATA ACGGGAGCAG CAGTGGAGCG 
CCTGCTGATC CTCCTGCTGC TGCTGCTGCT GCTGCTGCTC CTGTGCCAAC CTCAACTGCT 
CCAGCATCTG TTGCTCCTTC TGGCAGTACT TCTAGTAAAA CCTCGGAGAT CAGCCAGGAA 
GGCAATAGGT GGACCAGCAA AACCACTTCG AGCAGTAGCT GGAATCGTCC CACTTTGAAA 
GGAGGACTCT TCAGCCAGGC CAGCAGCAGT TCCCAGAGCC AAAGCACCTA CGATGACGGA 
AAGGGCAAGA CGAAGATCAG TTCGCTGTCT GTGAGATTGC AGCAGTCCAT CGAGGAAACA 
CCCAAGCTAA GCAACGGAAA TAGCTCCTCT AGCAAATCCC TGGTCCAGTC GCAGCGAAAG 
TCCACGGTGC AAACCATGAG CAGTTCCAAT GCCAGGAGCT TCACCAACCA GCAAGTACAA 
AGAACCAGCA CCACCTCTTC CAAGGTGATC TCCAGCAGCA CCAGAAGCAG TAACACCAGC 
TCCAGCAATT ATATAGCCTC CAACGCCAGC AACTCAATCT CGACCAGCGC CAGCAACCCA 
AGCTCCACCG GCGCCAGCAA TCCAATTACC ACCAGCGCCA GCAACGAATC GGCCAGCAGT 
CGTCGAGCCA AGTTCAGGAT AAATCAGATG AGTCGCGATG TGCCCGTTGG GCTGCCGGAC 
ACTCATCAAA CCGTGAATCT GGAGGAGGCG GCCAACACCA CCAAGGATTG CCTGCTGCAT 
TTGTTGGAGA AATACAACGA GACCCGCATC CGCAATCCGG TGGGTCGTCA CCAGAGCATC 
AGCGTGGACT GGCGGGTCAG CGACAATCTG GAGTACCGCT CCATGAGCTC CATCAACGCC 
TTCTTCCAGC GCCACAACCC CAATGGCGGC GGCCACAACG TACGCCACAT TCAAGCCCAG 
CTGGAGGAGA AGGCGGCGGG CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724821.2
CDS	377..3043
Translation	MIYIDDSEPEGVLEPAFPMRDVTINRNVDAHKHYDVLGEVGRGKFGTVYKCRDKANGLQLAAKFVPIPKREDKRNVEREVEIMNSLQHHLIIQLYAAYEYQKMMCVVLELIEGGELFDRVVDDEFVLTERVCRVFIRQVCEAMAFIHGNGIVHLDLKPENILVLTQKGNRIKIIDFGLARKFDPDKRLRVLFGTPEFVAPEVVNFDCISYGTDMWSVGVICYVLISGLSPFMGENDIETMSNVTIAKYDFEDECFNGISPECLDFIAKLLAKDLSTRMTAAECMKHKWLQQRPATAATATPITKAASAASKSRLKSVSPVTAPSESSEDSTETIEDEDDEEEVAVQQAKQKDQQQDEELANLCGDAELENKELDATKDNLKNFIVRWETHPNSPYVFDVEGNVIAPLSETSYPHPRRTHGADSLSSSRVCSPSPCDSISTLTDDERGDIEDLPEEDESRSAENESPRSVATPINESREKLFPTVATSSPSTPTPQHLFNENFDEFSGSQSTAQQQRSMKSYLHTFDRRNSDTTYLLGRRSSGERVNLADEIRKLSDHLLMLAEINTKLGDANNNGSSSGAPADPPAAAAAAAAPVPTSTAPASVAPSGSTSSKTSEISQEGNRWTSKTTSSSSWNRPTLKGGLFSQASSSSQSQSTYDDGKGKTKISSLSVRLQQSIEETPKLSNGNSSSSKSLVQSQRKSTVQTMSSSNARSFTNQQVQRTSTTSSKVISSSTRSSNTSSSNYIASNASNSISTSASNPSSTGASNPITTSASNESASSRRAKFRINQMSRDVPVGLPDTHQTVNLEEAANTTKDCLLHLLEKYNETRIRNPVGRHQSISVDWRVSDNLEYRSMSSINAFFQRHNPNGGGHNVRHIQAQLEEKAAGK

Target ORF information:

RefSeq Version	NM_165693.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spaghetti-squash activator (sqa), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165693.4

ORF Insert Sequence:

ATGATCTATA TAGATGACTC AGAGCCGGAA GGTGTTTTGG AACCGGCGTT TCCCATGCGC 
GATGTGACCA TAAATCGCAA CGTCGATGCC CATAAACATT ACGATGTGCT TGGTGAGGTG 
GGACGCGGAA AATTCGGTAC GGTGTACAAG TGCAGGGACA AGGCGAATGG ACTGCAGCTG 
GCGGCCAAGT TTGTGCCGAT CCCCAAGCGC GAGGACAAGC GGAATGTGGA GCGGGAGGTG 
GAGATAATGA ATTCATTGCA GCATCACCTC ATCATACAGT TGTACGCGGC ATACGAATAT 
CAGAAAATGA TGTGCGTCGT CCTGGAACTC ATCGAAGGAG GTGAGCTCTT TGATCGCGTG 
GTGGACGATG AGTTCGTCCT CACCGAACGA GTCTGCCGCG TCTTCATCCG CCAGGTGTGC 
GAGGCCATGG CCTTCATCCA CGGCAATGGA ATAGTCCACC TCGATCTGAA GCCCGAAAAC 
ATTTTGGTGC TCACACAGAA GGGCAACCGC ATCAAGATCA TTGACTTCGG ACTGGCTCGT 
AAATTTGATC CTGATAAGCG ACTCAGAGTT CTCTTCGGCA CTCCGGAATT TGTGGCGCCA 
GAAGTTGTGA ACTTTGACTG TATATCGTAT GGAACAGATA TGTGGAGTGT TGGCGTTATA 
TGCTATGTGC TCATCTCAGG ACTGTCGCCT TTCATGGGCG AGAACGACAT TGAAACAATG 
TCAAATGTAA CCATTGCAAA ATACGACTTC GAGGATGAAT GCTTTAATGG CATATCCCCC 
GAGTGTCTGG ATTTCATAGC CAAGCTGCTG GCCAAGGACC TGAGCACCCG GATGACAGCC 
GCCGAGTGCA TGAAGCACAA ATGGCTTCAG CAGCGGCCGG CCACCGCCGC CACGGCCACG 
CCCATCACAA AGGCCGCCTC CGCGGCGTCG AAGTCGCGCC TGAAGTCTGT GTCTCCGGTG 
ACAGCTCCCT CGGAATCCTC CGAGGACTCC ACGGAAACCA TTGAGGACGA AGACGACGAG 
GAAGAGGTTG CCGTGCAGCA GGCGAAGCAG AAAGACCAGC AGCAGGACGA GGAGCTGGCC 
AATCTCTGCG GCGACGCTGA ACTTGAGAAC AAAGAACTGG ATGCCACGAA GGACAACCTT 
AAGAACTTCA TCGTGCGCTG GGAGACGCAT CCGAACAGTC CGTACGTGTT CGACGTGGAG 
GGGAATGTGA TTGCTCCGCT GAGCGAGACG AGCTACCCCC ATCCCAGGAG GACCCACGGC 
GCGGACAGCC TTTCCTCCTC CAGAGTTTGT TCGCCTTCGC CCTGCGATTC AATATCCACT 
TTGACGGATG ACGAACGGGG AGATATTGAA GATCTTCCGG AAGAGGACGA ATCCCGCAGT 
GCGGAAAACG AGAGTCCTCG CTCGGTGGCC ACGCCCATCA ACGAGTCTCG GGAGAAGCTG 
TTCCCGACGG TAGCCACCTC CAGTCCGTCA ACTCCCACGC CGCAGCATCT CTTCAACGAG 
AACTTCGATG AGTTCTCTGG CAGCCAGTCC ACCGCCCAGC AGCAGCGCAG CATGAAGAGC 
TATCTGCACA CCTTCGATCG AAGGAACTCG GATACCACGT ACCTGCTGGG TCGGCGGAGT 
TCCGGAGAGC GGGTGAATCT GGCGGATGAG ATACGAAAGC TATCCGATCA CCTGCTCATG 
CTGGCCGAGA TCAACACCAA GCTTGGGGAT GCGAACAATA ACGGGAGCAG CAGTGGAGCG 
CCTGCTGATC CTCCTGCTGC TGCTGCTGCT GCTGCTGCTC CTGTGCCAAC CTCAACTGCT 
CCAGCATCTG TTGCTCCTTC TGGCAGTACT TCTAGTAAAA CCTCGGAGAT CAGCCAGGAA 
GGCAATAGGT GGACCAGCAA AACCACTTCG AGCAGTAGCT GGAATCGTCC CACTTTGAAA 
GGAGGACTCT TCAGCCAGGC CAGCAGCAGT TCCCAGAGCC AAAGCACCTA CGATGACGGA 
AAGGGCAAGA CGAAGATCAG TTCGCTGTCT GTGAGATTGC AGCAGTCCAT CGAGGAAACA 
CCCAAGCTAA GCAACGGAAA TAGCTCCTCT AGCAAATCCC TGGTCCAGTC GCAGCGAAAG 
TCCACGGTGC AAACCATGAG CAGTTCCAAT GCCAGGAGCT TCACCAACCA GCAAGTACAA 
AGAACCAGCA CCACCTCTTC CAAGGTGATC TCCAGCAGCA CCAGAAGCAG TAACACCAGC 
TCCAGCAATT ATATAGCCTC CAACGCCAGC AACTCAATCT CGACCAGCGC CAGCAACCCA 
AGCTCCACCG GCGCCAGCAA TCCAATTACC ACCAGCGCCA GCAACGAATC GGCCAGCAGT 
CGTCGAGCCA AGTTCAGGAT AAATCAGATG AGTCGCGATG TGCCCGTTGG GCTGCCGGAC 
ACTCATCAAA CCGTGAATCT GGAGGAGGCG GCCAACACCA CCAAGGATTG CCTGCTGCAT 
TTGTTGGAGA AATACAACGA GACCCGCATC CGCAATCCGG TGGGTCGTCA CCAGAGCATC 
AGCGTGGACT GGCGGGTCAG CGACAATCTG GAGTACCGCT CCATGAGCTC CATCAACGCC 
TTCTTCCAGC GCCACAACCC CAATGGCGGC GGCCACAACG TACGCCACAT TCAAGCCCAG 
CTGGAGGAGA AGGCGGCGGG CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sqa cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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