Pdk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdk
Entrez Gene ID	35970
Full Name	Pyruvate dehydrogenase kinase
Synonyms	BcDNA:LD09837,CG8808,DmPDK,DmPdk,Dmel\CG8808,PDK,pdk
General protein information	Preferred Names Pyruvate dehydrogenase kinase Names CG8808 gene product from transcript CG8808-RD CG8808-PA CG8808-PB CG8808-PC CG8808-PD Pdk-PA Pdk-PB Pdk-PC Pdk-PD pyruvate dehydrogenase kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45D5-45D7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273896.1	NP_001260825.1	pyruvate dehydrogenase kinase, isoform D
NM_057867.4	NP_477215.1	pyruvate dehydrogenase kinase, isoform A
NM_001103770.2	NP_001097240.1	pyruvate dehydrogenase kinase, isoform B
NM_001259295.2	NP_001246224.1	pyruvate dehydrogenase kinase, isoform C

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PDK3	NP_001095404.1
Mus musculus (house mouse)	Pdk3	NP_663605.1
Danio rerio (zebrafish)	pdk3a	XP_005162814.1
Drosophila melanogaster (fruit fly)	Pdk	NP_001097240.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010276	XP_319468.3
Neurospora crassa	NCU06760	XP_963532.2
Pan troglodytes (chimpanzee)	PDK3	XP_003317444.2
	PDK3	XP_002808569.1
Rattus norvegicus (Norway rat)	Pdk3	NP_001100051.1
Caenorhabditis elegans (roundworm)	pdhk-2	NP_498928.1
Schizosaccharomyces pombe (fission yeast)	pkp1	NP_593879.1
Gallus gallus (chicken)	PDK3	NP_001006259.1
Eremothecium gossypii	AGOS_AER270W	NP_985127.2
Magnaporthe oryzae (rice blast fungus)	MGG_07161	XP_003715359.1
Oryza sativa (rice)	Os07g0637300	NP_001060401.1
Canis lupus familiaris (dog)	PDK3	XP_005641265.1
Oryza sativa (rice)	Os03g0370000	NP_001050196.1
Homo sapiens (human)	PDK3	NP_001135858.1
Kluyveromyces lactis	KLLA0F27423g	XP_456300.1
Arabidopsis thaliana (thale cress)	PDK	NP_187300.3
Xenopus tropicalis (tropical clawed frog)	pdk3	NP_001107154.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pdk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09382	NM_001273896.1 Latest version!	Drosophila melanogaster pyruvate dehydrogenase kinase, transcript variant D (Pdk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09383	NM_001259295.2 Latest version!	Drosophila melanogaster pyruvate dehydrogenase kinase, transcript variant C (Pdk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09383	NM_057867.4 Latest version!	Drosophila melanogaster pyruvate dehydrogenase kinase, transcript variant A (Pdk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa12310	NM_001103770.2 Latest version!	Drosophila melanogaster pyruvate dehydrogenase kinase, transcript variant B (Pdk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdk ( NM_001273896.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260825.1 Drosophila melanogaster pyruvate dehydrogenase kinase, transcript variant D (Pdk), mRNA.

Price & Availability↑

CloneID	OFa09382
Clone ID Related Accession (Same CDS sequence)	NM_001273896.1
Accession Version	NM_001273896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate dehydrogenase kinase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCTGT TTCCAGTCCG ATTCTCAGCC GCCTCCTCGT CGATGGCGAG TTTGGCCAAA 
ATGCTCGACT TTTACTCGGG CTTCAACCCC TCGCCCCTCT CGATAAAGCA GTTCATGGAC 
TTTGGTCAGA ATGCCTGCGA GAAGAAATCG TACATATTCC TGCGCAAGGA ACTGCCTGTT 
CGACTGGCGA ATATCATGAA GGAGATCGCC CTTCTGCCGG ATAACCTGTT GCACACCAGA 
TCCGTAAGCG AAGTTAGTTC CTGGTATGTC AAGAGTTTCG AGGATGTGCT GGTGTACGAG 
AAAGCAGAGC CCACCCACGA CAATCTGCAA AAGTTTGTGG CCGATTTGGA TCTCATTAGG 
AATCGGCACA ACGATGTGGT GCAAACGATG GCCCAGGGTG TGATCGAAAT GAAGGAGAAC 
GAGGGCGGCC AGGTGGATGC GCCTACAGAG AGTTCCATTC AGTACTTTCT CGACAGGCTC 
TACATGTCTC GCATCAGCAT TCGAATGCTG ATAAATCAGC ACACCCTTCT CTTTGGCGGA 
AATCCACATG CGGGTGGCCG CCATATTGGT TGCCTGGATC CCGCCTGTGA TCTGTCGGAT 
GTGGTTCGCG ATGCTTACGA GAATGCGCGA TTCCTATGCG ATCAGTACTA CCTGACCAGT 
CCGGCATTGG AGATCCAGCA GCACAGCAGC GAGCCTGGAG ACAACCTGCC CATCCGCACC 
GTTTACGTGC CCTCGCACCT GTACTACATG CTTTTCGAGC TCTTCAAGAA CTCTATGCGA 
GCTGTTGTAG AGCACCATGG CCATGACAAC AATGACACAT TGCCCCCCTT GAAAGTAGCC 
ATTTGCAAGG GCAAGGAGGA CATCTGTGTG AAAATTTCTG ACCAGGGAGG CGGCATTCCC 
CGCTCCCAGA CCGATCAGCT CTTCAAGTAT ATGTACAGCA CAGCGCCGCA GCCCTCGAAG 
TCGGATCTGC ATACGGTGCC ATTAGCGGGC TATGGATACG GTCTGCCGAT CTCAAGGCTT 
TACGCCCGCT ATTTCCATGG CGACATTGTG CTGCTCTCCT GCGAAGGATT CGGAACAGAT 
GCAATCATCT ATCTAAAGGC TCTGTCCGAC GAAGCAAACG AATTGCTGCC GATCTTCAAC 
AAGACAAGCT CAAAGTTCTA TCGCGCCACC GTGCCCACGG GTGACTGGTC CAATCAGAAC 
TTTTCCAATC GTTGGCGTTA TTTATATGGT TTTTTAAAGA ATTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260825.1
CDS	812..2059
Translation	MRLFPVRFSAASSSMASLAKMLDFYSGFNPSPLSIKQFMDFGQNACEKKSYIFLRKELPVRLANIMKEIALLPDNLLHTRSVSEVSSWYVKSFEDVLVYEKAEPTHDNLQKFVADLDLIRNRHNDVVQTMAQGVIEMKENEGGQVDAPTESSIQYFLDRLYMSRISIRMLINQHTLLFGGNPHAGGRHIGCLDPACDLSDVVRDAYENARFLCDQYYLTSPALEIQQHSSEPGDNLPIRTVYVPSHLYYMLFELFKNSMRAVVEHHGHDNNDTLPPLKVAICKGKEDICVKISDQGGGIPRSQTDQLFKYMYSTAPQPSKSDLHTVPLAGYGYGLPISRLYARYFHGDIVLLSCEGFGTDAIIYLKALSDEANELLPIFNKTSSKFYRATVPTGDWSNQNFSNRWRYLYGFLKNS

Target ORF information:

RefSeq Version	NM_001273896.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate dehydrogenase kinase, transcript variant D (Pdk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273896.1

ORF Insert Sequence:

ATGCGCCTGT TTCCAGTCCG ATTCTCAGCC GCCTCCTCGT CGATGGCGAG TTTGGCCAAA 
ATGCTCGACT TTTACTCGGG CTTCAACCCC TCGCCCCTCT CGATAAAGCA GTTCATGGAC 
TTTGGTCAGA ATGCCTGCGA GAAGAAATCG TACATATTCC TGCGCAAGGA ACTGCCTGTT 
CGACTGGCGA ATATCATGAA GGAGATCGCC CTTCTGCCGG ATAACCTGTT GCACACCAGA 
TCCGTAAGCG AAGTTAGTTC CTGGTATGTC AAGAGTTTCG AGGATGTGCT GGTGTACGAG 
AAAGCAGAGC CCACCCACGA CAATCTGCAA AAGTTTGTGG CCGATTTGGA TCTCATTAGG 
AATCGGCACA ACGATGTGGT GCAAACGATG GCCCAGGGTG TGATCGAAAT GAAGGAGAAC 
GAGGGCGGCC AGGTGGATGC GCCTACAGAG AGTTCCATTC AGTACTTTCT CGACAGGCTC 
TACATGTCTC GCATCAGCAT TCGAATGCTG ATAAATCAGC ACACCCTTCT CTTTGGCGGA 
AATCCACATG CGGGTGGCCG CCATATTGGT TGCCTGGATC CCGCCTGTGA TCTGTCGGAT 
GTGGTTCGCG ATGCTTACGA GAATGCGCGA TTCCTATGCG ATCAGTACTA CCTGACCAGT 
CCGGCATTGG AGATCCAGCA GCACAGCAGC GAGCCTGGAG ACAACCTGCC CATCCGCACC 
GTTTACGTGC CCTCGCACCT GTACTACATG CTTTTCGAGC TCTTCAAGAA CTCTATGCGA 
GCTGTTGTAG AGCACCATGG CCATGACAAC AATGACACAT TGCCCCCCTT GAAAGTAGCC 
ATTTGCAAGG GCAAGGAGGA CATCTGTGTG AAAATTTCTG ACCAGGGAGG CGGCATTCCC 
CGCTCCCAGA CCGATCAGCT CTTCAAGTAT ATGTACAGCA CAGCGCCGCA GCCCTCGAAG 
TCGGATCTGC ATACGGTGCC ATTAGCGGGC TATGGATACG GTCTGCCGAT CTCAAGGCTT 
TACGCCCGCT ATTTCCATGG CGACATTGTG CTGCTCTCCT GCGAAGGATT CGGAACAGAT 
GCAATCATCT ATCTAAAGGC TCTGTCCGAC GAAGCAAACG AATTGCTGCC GATCTTCAAC 
AAGACAAGCT CAAAGTTCTA TCGCGCCACC GTGCCCACGG GTGACTGGTC CAATCAGAAC 
TTTTCCAATC GTTGGCGTTA TTTATATGGT TTTTTAAAGA ATTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdk ( NM_057867.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477215.1 Drosophila melanogaster pyruvate dehydrogenase kinase, transcript variant A (Pdk), mRNA.

Price & Availability↑

CloneID	OFa09383
Clone ID Related Accession (Same CDS sequence)	NM_057867.4 , NM_001259295.2
Accession Version	NM_057867.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate dehydrogenase kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057867.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCTGT TTCCAGTCCG ATTCTCAGCC GCCTCCTCGT CGATGGCGAG TTTGGCCAAA 
ATGCTCGACT TTTACTCGGG CTTCAACCCC TCGCCCCTCT CGATAAAGCA GTTCATGGAC 
TTTGGTCAGA ATGCCTGCGA GAAGAAATCG TACATATTCC TGCGCAAGGA ACTGCCTGTT 
CGACTGGCGA ATATCATGAA GGAGATCGCC CTTCTGCCGG ATAACCTGTT GCACACCAGA 
TCCGTAAGCG AAGTTAGTTC CTGGTATGTC AAGAGTTTCG AGGATGTGCT GGTGTACGAG 
AAAGCAGAGC CCACCCACGA CAATCTGCAA AAGTTTGTGG CCGATTTGGA TCTCATTAGG 
AATCGGCACA ACGATGTGGT GCAAACGATG GCCCAGGGTG TGATCGAAAT GAAGGAGAAC 
GAGGGCGGCC AGGTGGATGC GCCTACAGAG AGTTCCATTC AGTACTTTCT CGACAGGCTC 
TACATGTCTC GCATCAGCAT TCGAATGCTG ATAAATCAGC ACACCCTTCT CTTTGGCGGA 
AATCCACATG CGGGTGGCCG CCATATTGGT TGCCTGGATC CCGCCTGTGA TCTGTCGGAT 
GTGGTTCGCG ATGCTTACGA GAATGCGCGA TTCCTATGCG ATCAGTACTA CCTGACCAGT 
CCGGCATTGG AGATCCAGCA GCACAGCAGC GAGCCTGGAG ACAACCTGCC CATCCGCACC 
GTTTACGTGC CCTCGCACCT GTACTACATG CTTTTCGAGC TCTTCAAGAA CTCTATGCGA 
GCTGTTGTAG AGCACCATGG CCATGACAAC AATGACACAT TGCCCCCCTT GAAAGTAGCC 
ATTTGCAAGG GCAAGGAGGA CATCTGTGTG AAAATTTCTG ACCAGGGAGG CGGCATTCCC 
CGCTCCCAGA CCGATCAGCT CTTCAAGTAT ATGTACAGCA CAGCGCCGCA GCCCTCGAAG 
TCGGATCTGC ATACGGTGCC ATTAGCGGGC TATGGATACG GTCTGCCGAT CTCAAGGCTT 
TACGCCCGCT ATTTCCATGG CGACATTGTG CTGCTCTCCT GCGAAGGATT CGGAACAGAT 
GCAATCATCT ATCTAAAGGC TCTGTCCGAC GAAGCAAACG AATTGCTGCC GATCTTCAAC 
AAGACAAGCT CAAAGTTCTA TCGCGCCACC GTGCCCACGG GTGACTGGTC CAATCAGGTA 
AAATACGCTA AAAAGAAGAA GACGAGCGCT GTCAACCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477215.1
CDS	812..2053
Translation	MRLFPVRFSAASSSMASLAKMLDFYSGFNPSPLSIKQFMDFGQNACEKKSYIFLRKELPVRLANIMKEIALLPDNLLHTRSVSEVSSWYVKSFEDVLVYEKAEPTHDNLQKFVADLDLIRNRHNDVVQTMAQGVIEMKENEGGQVDAPTESSIQYFLDRLYMSRISIRMLINQHTLLFGGNPHAGGRHIGCLDPACDLSDVVRDAYENARFLCDQYYLTSPALEIQQHSSEPGDNLPIRTVYVPSHLYYMLFELFKNSMRAVVEHHGHDNNDTLPPLKVAICKGKEDICVKISDQGGGIPRSQTDQLFKYMYSTAPQPSKSDLHTVPLAGYGYGLPISRLYARYFHGDIVLLSCEGFGTDAIIYLKALSDEANELLPIFNKTSSKFYRATVPTGDWSNQVKYAKKKKTSAVNQ

Target ORF information:

RefSeq Version	NM_057867.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate dehydrogenase kinase, transcript variant A (Pdk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057867.4

ORF Insert Sequence:

ATGCGCCTGT TTCCAGTCCG ATTCTCAGCC GCCTCCTCGT CGATGGCGAG TTTGGCCAAA 
ATGCTCGACT TTTACTCGGG CTTCAACCCC TCGCCCCTCT CGATAAAGCA GTTCATGGAC 
TTTGGTCAGA ATGCCTGCGA GAAGAAATCG TACATATTCC TGCGCAAGGA ACTGCCTGTT 
CGACTGGCGA ATATCATGAA GGAGATCGCC CTTCTGCCGG ATAACCTGTT GCACACCAGA 
TCCGTAAGCG AAGTTAGTTC CTGGTATGTC AAGAGTTTCG AGGATGTGCT GGTGTACGAG 
AAAGCAGAGC CCACCCACGA CAATCTGCAA AAGTTTGTGG CCGATTTGGA TCTCATTAGG 
AATCGGCACA ACGATGTGGT GCAAACGATG GCCCAGGGTG TGATCGAAAT GAAGGAGAAC 
GAGGGCGGCC AGGTGGATGC GCCTACAGAG AGTTCCATTC AGTACTTTCT CGACAGGCTC 
TACATGTCTC GCATCAGCAT TCGAATGCTG ATAAATCAGC ACACCCTTCT CTTTGGCGGA 
AATCCACATG CGGGTGGCCG CCATATTGGT TGCCTGGATC CCGCCTGTGA TCTGTCGGAT 
GTGGTTCGCG ATGCTTACGA GAATGCGCGA TTCCTATGCG ATCAGTACTA CCTGACCAGT 
CCGGCATTGG AGATCCAGCA GCACAGCAGC GAGCCTGGAG ACAACCTGCC CATCCGCACC 
GTTTACGTGC CCTCGCACCT GTACTACATG CTTTTCGAGC TCTTCAAGAA CTCTATGCGA 
GCTGTTGTAG AGCACCATGG CCATGACAAC AATGACACAT TGCCCCCCTT GAAAGTAGCC 
ATTTGCAAGG GCAAGGAGGA CATCTGTGTG AAAATTTCTG ACCAGGGAGG CGGCATTCCC 
CGCTCCCAGA CCGATCAGCT CTTCAAGTAT ATGTACAGCA CAGCGCCGCA GCCCTCGAAG 
TCGGATCTGC ATACGGTGCC ATTAGCGGGC TATGGATACG GTCTGCCGAT CTCAAGGCTT 
TACGCCCGCT ATTTCCATGG CGACATTGTG CTGCTCTCCT GCGAAGGATT CGGAACAGAT 
GCAATCATCT ATCTAAAGGC TCTGTCCGAC GAAGCAAACG AATTGCTGCC GATCTTCAAC 
AAGACAAGCT CAAAGTTCTA TCGCGCCACC GTGCCCACGG GTGACTGGTC CAATCAGGTA 
AAATACGCTA AAAAGAAGAA GACGAGCGCT GTCAACCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdk ( NM_001259295.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246224.1 Drosophila melanogaster pyruvate dehydrogenase kinase, transcript variant C (Pdk), mRNA.

Price & Availability↑

CloneID	OFa09383
Clone ID Related Accession (Same CDS sequence)	NM_057867.4 , NM_001259295.2
Accession Version	NM_001259295.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate dehydrogenase kinase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259295.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCTGT TTCCAGTCCG ATTCTCAGCC GCCTCCTCGT CGATGGCGAG TTTGGCCAAA 
ATGCTCGACT TTTACTCGGG CTTCAACCCC TCGCCCCTCT CGATAAAGCA GTTCATGGAC 
TTTGGTCAGA ATGCCTGCGA GAAGAAATCG TACATATTCC TGCGCAAGGA ACTGCCTGTT 
CGACTGGCGA ATATCATGAA GGAGATCGCC CTTCTGCCGG ATAACCTGTT GCACACCAGA 
TCCGTAAGCG AAGTTAGTTC CTGGTATGTC AAGAGTTTCG AGGATGTGCT GGTGTACGAG 
AAAGCAGAGC CCACCCACGA CAATCTGCAA AAGTTTGTGG CCGATTTGGA TCTCATTAGG 
AATCGGCACA ACGATGTGGT GCAAACGATG GCCCAGGGTG TGATCGAAAT GAAGGAGAAC 
GAGGGCGGCC AGGTGGATGC GCCTACAGAG AGTTCCATTC AGTACTTTCT CGACAGGCTC 
TACATGTCTC GCATCAGCAT TCGAATGCTG ATAAATCAGC ACACCCTTCT CTTTGGCGGA 
AATCCACATG CGGGTGGCCG CCATATTGGT TGCCTGGATC CCGCCTGTGA TCTGTCGGAT 
GTGGTTCGCG ATGCTTACGA GAATGCGCGA TTCCTATGCG ATCAGTACTA CCTGACCAGT 
CCGGCATTGG AGATCCAGCA GCACAGCAGC GAGCCTGGAG ACAACCTGCC CATCCGCACC 
GTTTACGTGC CCTCGCACCT GTACTACATG CTTTTCGAGC TCTTCAAGAA CTCTATGCGA 
GCTGTTGTAG AGCACCATGG CCATGACAAC AATGACACAT TGCCCCCCTT GAAAGTAGCC 
ATTTGCAAGG GCAAGGAGGA CATCTGTGTG AAAATTTCTG ACCAGGGAGG CGGCATTCCC 
CGCTCCCAGA CCGATCAGCT CTTCAAGTAT ATGTACAGCA CAGCGCCGCA GCCCTCGAAG 
TCGGATCTGC ATACGGTGCC ATTAGCGGGC TATGGATACG GTCTGCCGAT CTCAAGGCTT 
TACGCCCGCT ATTTCCATGG CGACATTGTG CTGCTCTCCT GCGAAGGATT CGGAACAGAT 
GCAATCATCT ATCTAAAGGC TCTGTCCGAC GAAGCAAACG AATTGCTGCC GATCTTCAAC 
AAGACAAGCT CAAAGTTCTA TCGCGCCACC GTGCCCACGG GTGACTGGTC CAATCAGGTA 
AAATACGCTA AAAAGAAGAA GACGAGCGCT GTCAACCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246224.1
CDS	812..2053
Translation	MRLFPVRFSAASSSMASLAKMLDFYSGFNPSPLSIKQFMDFGQNACEKKSYIFLRKELPVRLANIMKEIALLPDNLLHTRSVSEVSSWYVKSFEDVLVYEKAEPTHDNLQKFVADLDLIRNRHNDVVQTMAQGVIEMKENEGGQVDAPTESSIQYFLDRLYMSRISIRMLINQHTLLFGGNPHAGGRHIGCLDPACDLSDVVRDAYENARFLCDQYYLTSPALEIQQHSSEPGDNLPIRTVYVPSHLYYMLFELFKNSMRAVVEHHGHDNNDTLPPLKVAICKGKEDICVKISDQGGGIPRSQTDQLFKYMYSTAPQPSKSDLHTVPLAGYGYGLPISRLYARYFHGDIVLLSCEGFGTDAIIYLKALSDEANELLPIFNKTSSKFYRATVPTGDWSNQVKYAKKKKTSAVNQ

Target ORF information:

RefSeq Version	NM_001259295.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate dehydrogenase kinase, transcript variant C (Pdk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259295.2

ORF Insert Sequence:

ATGCGCCTGT TTCCAGTCCG ATTCTCAGCC GCCTCCTCGT CGATGGCGAG TTTGGCCAAA 
ATGCTCGACT TTTACTCGGG CTTCAACCCC TCGCCCCTCT CGATAAAGCA GTTCATGGAC 
TTTGGTCAGA ATGCCTGCGA GAAGAAATCG TACATATTCC TGCGCAAGGA ACTGCCTGTT 
CGACTGGCGA ATATCATGAA GGAGATCGCC CTTCTGCCGG ATAACCTGTT GCACACCAGA 
TCCGTAAGCG AAGTTAGTTC CTGGTATGTC AAGAGTTTCG AGGATGTGCT GGTGTACGAG 
AAAGCAGAGC CCACCCACGA CAATCTGCAA AAGTTTGTGG CCGATTTGGA TCTCATTAGG 
AATCGGCACA ACGATGTGGT GCAAACGATG GCCCAGGGTG TGATCGAAAT GAAGGAGAAC 
GAGGGCGGCC AGGTGGATGC GCCTACAGAG AGTTCCATTC AGTACTTTCT CGACAGGCTC 
TACATGTCTC GCATCAGCAT TCGAATGCTG ATAAATCAGC ACACCCTTCT CTTTGGCGGA 
AATCCACATG CGGGTGGCCG CCATATTGGT TGCCTGGATC CCGCCTGTGA TCTGTCGGAT 
GTGGTTCGCG ATGCTTACGA GAATGCGCGA TTCCTATGCG ATCAGTACTA CCTGACCAGT 
CCGGCATTGG AGATCCAGCA GCACAGCAGC GAGCCTGGAG ACAACCTGCC CATCCGCACC 
GTTTACGTGC CCTCGCACCT GTACTACATG CTTTTCGAGC TCTTCAAGAA CTCTATGCGA 
GCTGTTGTAG AGCACCATGG CCATGACAAC AATGACACAT TGCCCCCCTT GAAAGTAGCC 
ATTTGCAAGG GCAAGGAGGA CATCTGTGTG AAAATTTCTG ACCAGGGAGG CGGCATTCCC 
CGCTCCCAGA CCGATCAGCT CTTCAAGTAT ATGTACAGCA CAGCGCCGCA GCCCTCGAAG 
TCGGATCTGC ATACGGTGCC ATTAGCGGGC TATGGATACG GTCTGCCGAT CTCAAGGCTT 
TACGCCCGCT ATTTCCATGG CGACATTGTG CTGCTCTCCT GCGAAGGATT CGGAACAGAT 
GCAATCATCT ATCTAAAGGC TCTGTCCGAC GAAGCAAACG AATTGCTGCC GATCTTCAAC 
AAGACAAGCT CAAAGTTCTA TCGCGCCACC GTGCCCACGG GTGACTGGTC CAATCAGGTA 
AAATACGCTA AAAAGAAGAA GACGAGCGCT GTCAACCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdk ( NM_001103770.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097240.1 Drosophila melanogaster pyruvate dehydrogenase kinase, transcript variant B (Pdk), mRNA.

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CloneID	OFa12310
Clone ID Related Accession (Same CDS sequence)	NM_001103770.2
Accession Version	NM_001103770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate dehydrogenase kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001103770.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCTGT TTCCAGTCCG ATTCTCAGCC GCCTCCTCGT CGATGGCGAG TTTGGCCAAA 
ATGCTCGACT TTTACTCGGG CTTCAACCCC TCGCCCCTCT CGATAAAGCA GTTCATGGAC 
TTTGGTCAGA ATGCCTGCGA GAAGAAATCG TACATATTCC TGCGCAAGGA ACTGCCTGTT 
CGACTGGCGA ATATCATGAA GGAGATCGCC CTTCTGCCGG ATAACCTGTT GCACACCAGA 
TCCGTAAGCG AAGTTAGTTC CTGGTATGTC AAGAGTTTCG AGGATGTGCT GGTGTACGAG 
AAAGCAGAGC CCACCCACGA CAATCTGCAA AAGTTTGTGG CCGATTTGGA TCTCATTAGG 
AATCGGCACA ACGATGTGGT GCAAACGATG GCCCAGGGTG TGATCGAAAT GAAGGAGAAC 
GAGGGCGGCC AGGTGGATGC GCCTACAGAG AGTTCCATTC AGTACTTTCT CGACAGGCTC 
TACATGTCTC GCATCAGCAT TCGAATGCTG ATAAATCAGC ACACCCTTCT CTTTGGCGGA 
AATCCACATG CGGGTGGCCG CCATATTGGT TGCCTGGATC CCGCCTGTGA TCTGTCGGAT 
GTGGTTCGCG ATGCTTACGA GAATGCGCGA TTCCTATGCG ATCAGTACTA CCTGACCAGT 
CCGGCATTGG AGATCCAGCA GCACAGCAGC GAGCCTGGAG ACAACCTGCC CATCCGCACC 
GTTTACGTGC CCTCGCACCT GTACTACATG CTTTTCGAGC TCTTCAAGAA CTCTATGCGA 
GCTGTTGTAG AGCACCATGG CCATGACAAC AATGACACAT TGCCCCCCTT GAAAGTAGCC 
ATTTGCAAGG GCAAGGAGGA CATCTGTGTG AAAATTTCTG ACCAGGGAGG CGGCATTCCC 
CGCTCCCAGA CCGATCAGCT CTTCAAGTAT ATGTACAGCA CAGCGCCGCA GCCCTCGAAG 
TCGGATCTGC ATACGGTGCC ATTAGCGGGC TATGGATACG GTCTGCCGAT CTCAAGGCTT 
TACGCCCGCT ATTTCCATGG CGACATTGTG CTGCTCTCCT GCGAAGGATT CGGAACAGAT 
GCAATCATCT ATCTAAAGGC TCTGTCCGAC GAAGCAAACG AATTGCTGCC GATCTTCAAC 
AAGACAAGCT CAAAGTTCTA TCGCGCCACC GTGCCCACGG GTGACTGGTC CAATCAGAGC 
TCCGATATGA ACGCACGCCA GTTGTCGGCC ACCACACCCA AGCGCTGCGA CTTTCTTCAG 
GACGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097240.1
CDS	812..2080
Translation	MRLFPVRFSAASSSMASLAKMLDFYSGFNPSPLSIKQFMDFGQNACEKKSYIFLRKELPVRLANIMKEIALLPDNLLHTRSVSEVSSWYVKSFEDVLVYEKAEPTHDNLQKFVADLDLIRNRHNDVVQTMAQGVIEMKENEGGQVDAPTESSIQYFLDRLYMSRISIRMLINQHTLLFGGNPHAGGRHIGCLDPACDLSDVVRDAYENARFLCDQYYLTSPALEIQQHSSEPGDNLPIRTVYVPSHLYYMLFELFKNSMRAVVEHHGHDNNDTLPPLKVAICKGKEDICVKISDQGGGIPRSQTDQLFKYMYSTAPQPSKSDLHTVPLAGYGYGLPISRLYARYFHGDIVLLSCEGFGTDAIIYLKALSDEANELLPIFNKTSSKFYRATVPTGDWSNQSSDMNARQLSATTPKRCDFLQDA

Target ORF information:

RefSeq Version	NM_001103770.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate dehydrogenase kinase, transcript variant B (Pdk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103770.2

ORF Insert Sequence:

ATGCGCCTGT TTCCAGTCCG ATTCTCAGCC GCCTCCTCGT CGATGGCGAG TTTGGCCAAA 
ATGCTCGACT TTTACTCGGG CTTCAACCCC TCGCCCCTCT CGATAAAGCA GTTCATGGAC 
TTTGGTCAGA ATGCCTGCGA GAAGAAATCG TACATATTCC TGCGCAAGGA ACTGCCTGTT 
CGACTGGCGA ATATCATGAA GGAGATCGCC CTTCTGCCGG ATAACCTGTT GCACACCAGA 
TCCGTAAGCG AAGTTAGTTC CTGGTATGTC AAGAGTTTCG AGGATGTGCT GGTGTACGAG 
AAAGCAGAGC CCACCCACGA CAATCTGCAA AAGTTTGTGG CCGATTTGGA TCTCATTAGG 
AATCGGCACA ACGATGTGGT GCAAACGATG GCCCAGGGTG TGATCGAAAT GAAGGAGAAC 
GAGGGCGGCC AGGTGGATGC GCCTACAGAG AGTTCCATTC AGTACTTTCT CGACAGGCTC 
TACATGTCTC GCATCAGCAT TCGAATGCTG ATAAATCAGC ACACCCTTCT CTTTGGCGGA 
AATCCACATG CGGGTGGCCG CCATATTGGT TGCCTGGATC CCGCCTGTGA TCTGTCGGAT 
GTGGTTCGCG ATGCTTACGA GAATGCGCGA TTCCTATGCG ATCAGTACTA CCTGACCAGT 
CCGGCATTGG AGATCCAGCA GCACAGCAGC GAGCCTGGAG ACAACCTGCC CATCCGCACC 
GTTTACGTGC CCTCGCACCT GTACTACATG CTTTTCGAGC TCTTCAAGAA CTCTATGCGA 
GCTGTTGTAG AGCACCATGG CCATGACAAC AATGACACAT TGCCCCCCTT GAAAGTAGCC 
ATTTGCAAGG GCAAGGAGGA CATCTGTGTG AAAATTTCTG ACCAGGGAGG CGGCATTCCC 
CGCTCCCAGA CCGATCAGCT CTTCAAGTAT ATGTACAGCA CAGCGCCGCA GCCCTCGAAG 
TCGGATCTGC ATACGGTGCC ATTAGCGGGC TATGGATACG GTCTGCCGAT CTCAAGGCTT 
TACGCCCGCT ATTTCCATGG CGACATTGTG CTGCTCTCCT GCGAAGGATT CGGAACAGAT 
GCAATCATCT ATCTAAAGGC TCTGTCCGAC GAAGCAAACG AATTGCTGCC GATCTTCAAC 
AAGACAAGCT CAAAGTTCTA TCGCGCCACC GTGCCCACGG GTGACTGGTC CAATCAGAGC 
TCCGATATGA ACGCACGCCA GTTGTCGGCC ACCACACCCA AGCGCTGCGA CTTTCTTCAG 
GACGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pdk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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