wun cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wun
Entrez Gene ID	35966
Full Name	wunen
Synonyms	CG8804,Dmel\CG8804,LPP-like,Wun,Wunen,l(2)k16806
General protein information	Preferred Names wunen Names CG8804 gene product from transcript CG8804-RB CG8804-PA CG8804-PB CG8804-PC wun-PA wun-PB wun-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45D3-45D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259294.2	NP_001246223.1	wunen, isoform C
NM_165674.3	NP_724787.1	wunen, isoform B
NM_057845.5	NP_477193.1	wunen, isoform A

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	CELE_F53C3.13	NP_872026.1
Homo sapiens (human)	PPAP2A	NP_795714.1
Drosophila melanogaster (fruit fly)	wun	NP_724787.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012445	XP_313220.4
Pan troglodytes (chimpanzee)	PPAP2A	XP_001146001.1
Danio rerio (zebrafish)	LOC569053	XP_005155515.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Wunen mutants have cardioblast joining defects and loose pericardial cells in the developing heart.
Title: Drosophila heart cell movement to the midline occurs through both cell autonomous migration and dorsal closure.

The germ cell migratory path can be primarily accounted for by Wunen expression.
Title: Quantifying the range of a lipid phosphate signal in vivo.

germ cell survival and directionality of migration are controlled by two lipid phosphate phosphatases (LPP), wunen (wun) and wunen-2 (wun2). wun wun2 double mutant analysis reveals that the two genes act redundantly in primordial germ cells.
Title: Lipid phosphate phosphatase activity regulates dispersal and bilateral sorting of embryonic germ cells in Drosophila.

Drosophila melanogaster lipid phosphate phosphatases Wunen and Wunen 2 have roles in lateral migration and germ cell elimination
Title: Control of lateral migration and germ cell elimination by the Drosophila melanogaster lipid phosphate phosphatases Wunen and Wunen 2.

there are marked differences in biochemical activity between fly Wun and mammalian lipid phosphate phosphatase (LPPs), with Wun having a narrower activity range than has been reported for the mammalian LPPs
Title: Fly and mammalian lipid phosphate phosphatase isoforms differ in activity both in vitro and in vivo.

The following wun gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wun cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09380	NM_001259294.2 Latest version!	Drosophila melanogaster wunen (wun), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa12306	NM_165674.3 Latest version!	Drosophila melanogaster wunen (wun), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa12307	NM_057845.5 Latest version!	Drosophila melanogaster wunen (wun), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wun ( NM_001259294.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246223.1 Drosophila melanogaster wunen (wun), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa09380
Clone ID Related Accession (Same CDS sequence)	NM_001259294.2
Accession Version	NM_001259294.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wunen, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259294.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGCCG TGAAAATAAT AATGAGTACC GAAACGTCGG CGAGCGAGAC AACACCGCTG 
CGGCGGTCGG AAAACGAAAC ACCTGACCAT AAAGAACTGG CCCAAAGTAA CAGCAACAGT 
CGACAAACAA CAGTTAATAG CAACAACAAT AACTACAGTA ACTCGGTGCA AGTGCGTCTG 
CAGGAACAGG ACAGAGATAG CGACAGCGAG CAACAACAAC ATACTGCCAC CATCACCATG 
GATACCAACA AAAGAATCCT CTGCCGCGTG GGCTTGGATG TTTTAATCTT GCTCTGCGCT 
GGATTTCCCA TACTGCTGTT TTTCCTGCTG GGCGAGCCGT ACAAGCGGGG CTTCTTCTGC 
GATGACGAGT CACTGAAGCA CCCGTTCCAC GATTCTACGG TCCGGAATTG GATGCTATAC 
TTCATCGGAG CTGTGATACC AGTCGGCGTG ATATTCATCG TGGAGGTCAT CATTTCACAG 
AACAAGGCCA AGCAGGATAA TGGCAATGCA ACCAGTCGAA GGTACGTCTT TATGAACTAC 
GAACTGCCCG ACTGGATGAT CGAGTGCTAC AAGAAGATCG GCATCTATGC CTTCGGCGCT 
GTGCTCAGCC AGCTGACCAC GGATATAGCC AAGTACTCCA TCGGCCGACT GCGTCCCCAT 
TTCATCGCGG TGTGCCAGCC TCAAATGGCC GACGGCAGCA CCTGCGACGA TGCGATCAAT 
GCGGGAAAAT ACATCCAGGA GTTCACATGC AAGGGAGTTG GATCGTCGGC GCGCATGCTC 
AAAGAGATGC GCCTCTCCTT CCCCAGTGGC CACTCCAGCT TCACCTTCTT CGCCATGGTC 
TATCTGGCGC TCTACCTGCA GGCTCGCATG ACCTGGCGGG GCTCCAAGCT GCTGCGTCAC 
CTTCTCCAGT TCCTGTTCAT CATGGTGGCC TGGTACACAG CCCTAAGTCG CGTATCGGAC 
TACAAGCACC ACTGGTCCGA TGTGCTGGCA GGATCGCTTA TTGGCTCCAT AAGCGCACTG 
GTCGTGAGCT ACGGTTTGCC CAAGAGTTGG TTCAACTTTC AAACAGTTTG GCCCGCCAAA 
AGTAAGGCCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246223.1
CDS	383..1477
Translation	MPAVKIIMSTETSASETTPLRRSENETPDHKELAQSNSNSRQTTVNSNNNNYSNSVQVRLQEQDRDSDSEQQQHTATITMDTNKRILCRVGLDVLILLCAGFPILLFFLLGEPYKRGFFCDDESLKHPFHDSTVRNWMLYFIGAVIPVGVIFIVEVIISQNKAKQDNGNATSRRYVFMNYELPDWMIECYKKIGIYAFGAVLSQLTTDIAKYSIGRLRPHFIAVCQPQMADGSTCDDAINAGKYIQEFTCKGVGSSARMLKEMRLSFPSGHSSFTFFAMVYLALYLQARMTWRGSKLLRHLLQFLFIMVAWYTALSRVSDYKHHWSDVLAGSLIGSISALVVSYGLPKSWFNFQTVWPAKSKAK

Target ORF information:

RefSeq Version	NM_001259294.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wunen (wun), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259294.2

ORF Insert Sequence:

ATGCCAGCCG TGAAAATAAT AATGAGTACC GAAACGTCGG CGAGCGAGAC AACACCGCTG 
CGGCGGTCGG AAAACGAAAC ACCTGACCAT AAAGAACTGG CCCAAAGTAA CAGCAACAGT 
CGACAAACAA CAGTTAATAG CAACAACAAT AACTACAGTA ACTCGGTGCA AGTGCGTCTG 
CAGGAACAGG ACAGAGATAG CGACAGCGAG CAACAACAAC ATACTGCCAC CATCACCATG 
GATACCAACA AAAGAATCCT CTGCCGCGTG GGCTTGGATG TTTTAATCTT GCTCTGCGCT 
GGATTTCCCA TACTGCTGTT TTTCCTGCTG GGCGAGCCGT ACAAGCGGGG CTTCTTCTGC 
GATGACGAGT CACTGAAGCA CCCGTTCCAC GATTCTACGG TCCGGAATTG GATGCTATAC 
TTCATCGGAG CTGTGATACC AGTCGGCGTG ATATTCATCG TGGAGGTCAT CATTTCACAG 
AACAAGGCCA AGCAGGATAA TGGCAATGCA ACCAGTCGAA GGTACGTCTT TATGAACTAC 
GAACTGCCCG ACTGGATGAT CGAGTGCTAC AAGAAGATCG GCATCTATGC CTTCGGCGCT 
GTGCTCAGCC AGCTGACCAC GGATATAGCC AAGTACTCCA TCGGCCGACT GCGTCCCCAT 
TTCATCGCGG TGTGCCAGCC TCAAATGGCC GACGGCAGCA CCTGCGACGA TGCGATCAAT 
GCGGGAAAAT ACATCCAGGA GTTCACATGC AAGGGAGTTG GATCGTCGGC GCGCATGCTC 
AAAGAGATGC GCCTCTCCTT CCCCAGTGGC CACTCCAGCT TCACCTTCTT CGCCATGGTC 
TATCTGGCGC TCTACCTGCA GGCTCGCATG ACCTGGCGGG GCTCCAAGCT GCTGCGTCAC 
CTTCTCCAGT TCCTGTTCAT CATGGTGGCC TGGTACACAG CCCTAAGTCG CGTATCGGAC 
TACAAGCACC ACTGGTCCGA TGTGCTGGCA GGATCGCTTA TTGGCTCCAT AAGCGCACTG 
GTCGTGAGCT ACGGTTTGCC CAAGAGTTGG TTCAACTTTC AAACAGTTTG GCCCGCCAAA 
AGTAAGGCCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wun ( NM_165674.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724787.1 Drosophila melanogaster wunen (wun), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12306
Clone ID Related Accession (Same CDS sequence)	NM_165674.3
Accession Version	NM_165674.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wunen, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165674.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGCCG TGAAAATAAT AATGAGTACC GAAACGTCGG CGAGCGAGAC AACACCGCTG 
CGGCGGTCGG AAAACGAAAC ACCTGACCAT AAAGAACTGG CCCAAAGTAA CAGCAACAGT 
CGACAAACAA CAGTTAATAG CAACAACAAT AACTACAGTA ACTCGGTGCA AGTGCGTCTG 
CAGGAACAGG ACAGAGATAG CGACAGCGAG CAACAACAAC ATACTGCCAC CATCACCATG 
GATACCAACA AAAGAATCCT CTGCCGCGTG GGCTTGGATG TTTTAATCTT GCTCTGCGCT 
GGATTTCCCA TACTGCTGTT TTTCCTGCTG GGCGAGCCGT ACAAGCGGGG CTTCTTCTGC 
GATGACGAGT CACTGAAGCA CCCGTTCCAC GATTCTACGG TCCGGAATTG GATGCTATAC 
TTCATCGGAG CTGTGATACC AGTCGGCGTG ATATTCATCG TGGAGGTCAT CATTTCACAG 
AACAAGGCCA AGCAGGATAA TGGCAATGCA ACCAGTCGAA GGTACGTCTT TATGAACTAC 
GAACTGCCCG ACTGGATGAT CGAGTGCTAC AAGAAGATCG GCATCTATGC CTTCGGCGCT 
GTGCTCAGCC AGCTGACCAC GGATATAGCC AAGTACTCCA TCGGCCGACT GCGTCCCCAT 
TTCATCGCGG TGTGCCAGCC TCAAATGGCC GACGGCAGCA CCTGCGACGA TGCGATCAAT 
GCGGGAAAAT ACATCCAGGA GTTCACATGC AAGGGAGTTG GATCGTCGGC GCGCATGCTC 
AAAGAGATGC GCCTCTCCTT CCCCAGTGGC CACTCCAGCT TCACCTTCTT CGCCATGGTC 
TATCTGGCGC TCTACCTGCA GGCTCGCATG ACCTGGCGGG GCTCCAAGCT GCTGCGTCAC 
CTTCTCCAGT TCCTGTTCAT CATGGTGGCC TGGTACACAG CCCTAAGTCG CGTATCGGAC 
TACAAGCACC ACTGGTCCGA TGTGCTGGCA GGATCGCTTA TTGGCTCCAT AAGCGCACTG 
GTCGTGGCCA ACTATGTCTC CGACTTGTTT CAAAAGCCCA ACACGAAGCC CTATTTGGCG 
CGTACAGTGC AAGACATGAA TGCATCGCCG GCCCAGGCCA TAACGATTAC TACAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724787.1
CDS	383..1522
Translation	MPAVKIIMSTETSASETTPLRRSENETPDHKELAQSNSNSRQTTVNSNNNNYSNSVQVRLQEQDRDSDSEQQQHTATITMDTNKRILCRVGLDVLILLCAGFPILLFFLLGEPYKRGFFCDDESLKHPFHDSTVRNWMLYFIGAVIPVGVIFIVEVIISQNKAKQDNGNATSRRYVFMNYELPDWMIECYKKIGIYAFGAVLSQLTTDIAKYSIGRLRPHFIAVCQPQMADGSTCDDAINAGKYIQEFTCKGVGSSARMLKEMRLSFPSGHSSFTFFAMVYLALYLQARMTWRGSKLLRHLLQFLFIMVAWYTALSRVSDYKHHWSDVLAGSLIGSISALVVANYVSDLFQKPNTKPYLARTVQDMNASPAQAITITTN

Target ORF information:

RefSeq Version	NM_165674.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wunen (wun), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165674.3

ORF Insert Sequence:

ATGCCAGCCG TGAAAATAAT AATGAGTACC GAAACGTCGG CGAGCGAGAC AACACCGCTG 
CGGCGGTCGG AAAACGAAAC ACCTGACCAT AAAGAACTGG CCCAAAGTAA CAGCAACAGT 
CGACAAACAA CAGTTAATAG CAACAACAAT AACTACAGTA ACTCGGTGCA AGTGCGTCTG 
CAGGAACAGG ACAGAGATAG CGACAGCGAG CAACAACAAC ATACTGCCAC CATCACCATG 
GATACCAACA AAAGAATCCT CTGCCGCGTG GGCTTGGATG TTTTAATCTT GCTCTGCGCT 
GGATTTCCCA TACTGCTGTT TTTCCTGCTG GGCGAGCCGT ACAAGCGGGG CTTCTTCTGC 
GATGACGAGT CACTGAAGCA CCCGTTCCAC GATTCTACGG TCCGGAATTG GATGCTATAC 
TTCATCGGAG CTGTGATACC AGTCGGCGTG ATATTCATCG TGGAGGTCAT CATTTCACAG 
AACAAGGCCA AGCAGGATAA TGGCAATGCA ACCAGTCGAA GGTACGTCTT TATGAACTAC 
GAACTGCCCG ACTGGATGAT CGAGTGCTAC AAGAAGATCG GCATCTATGC CTTCGGCGCT 
GTGCTCAGCC AGCTGACCAC GGATATAGCC AAGTACTCCA TCGGCCGACT GCGTCCCCAT 
TTCATCGCGG TGTGCCAGCC TCAAATGGCC GACGGCAGCA CCTGCGACGA TGCGATCAAT 
GCGGGAAAAT ACATCCAGGA GTTCACATGC AAGGGAGTTG GATCGTCGGC GCGCATGCTC 
AAAGAGATGC GCCTCTCCTT CCCCAGTGGC CACTCCAGCT TCACCTTCTT CGCCATGGTC 
TATCTGGCGC TCTACCTGCA GGCTCGCATG ACCTGGCGGG GCTCCAAGCT GCTGCGTCAC 
CTTCTCCAGT TCCTGTTCAT CATGGTGGCC TGGTACACAG CCCTAAGTCG CGTATCGGAC 
TACAAGCACC ACTGGTCCGA TGTGCTGGCA GGATCGCTTA TTGGCTCCAT AAGCGCACTG 
GTCGTGGCCA ACTATGTCTC CGACTTGTTT CAAAAGCCCA ACACGAAGCC CTATTTGGCG 
CGTACAGTGC AAGACATGAA TGCATCGCCG GCCCAGGCCA TAACGATTAC TACAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wun ( NM_057845.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477193.1 Drosophila melanogaster wunen (wun), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12307
Clone ID Related Accession (Same CDS sequence)	NM_057845.5
Accession Version	NM_057845.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wunen, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057845.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATACCA ACAAAAGAAT CCTCTGCCGC GTGGGCTTGG ATGTTTTAAT CTTGCTCTGC 
GCTGGATTTC CCATACTGCT GTTTTTCCTG CTGGGCGAGC CGTACAAGCG GGGCTTCTTC 
TGCGATGACG AGTCACTGAA GCACCCGTTC CACGATTCTA CGGTCCGGAA TTGGATGCTA 
TACTTCATCG GAGCTGTGAT ACCAGTCGGC GTGATATTCA TCGTGGAGGT CATCATTTCA 
CAGAACAAGG CCAAGCAGGA TAATGGCAAT GCAACCAGTC GAAGGTACGT CTTTATGAAC 
TACGAACTGC CCGACTGGAT GATCGAGTGC TACAAGAAGA TCGGCATCTA TGCCTTCGGC 
GCTGTGCTCA GCCAGCTGAC CACGGATATA GCCAAGTACT CCATCGGCCG ACTGCGTCCC 
CATTTCATCG CGGTGTGCCA GCCTCAAATG GCCGACGGCA GCACCTGCGA CGATGCGATC 
AATGCGGGAA AATACATCCA GGAGTTCACA TGCAAGGGAG TTGGATCGTC GGCGCGCATG 
CTCAAAGAGA TGCGCCTCTC CTTCCCCAGT GGCCACTCCA GCTTCACCTT CTTCGCCATG 
GTCTATCTGG CGCTCTACCT GCAGGCTCGC ATGACCTGGC GGGGCTCCAA GCTGCTGCGT 
CACCTTCTCC AGTTCCTGTT CATCATGGTG GCCTGGTACA CAGCCCTAAG TCGCGTATCG 
GACTACAAGC ACCACTGGTC CGATGTGCTG GCAGGATCGC TTATTGGCTC CATAAGCGCA 
CTGGTCGTGG CCAACTATGT CTCCGACTTG TTTCAAAAGC CCAACACGAA GCCCTATTTG 
GCGCGTACAG TGCAAGACAT GAATGCATCG CCGGCCCAGG CCATAACGAT TACTACAAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477193.1
CDS	220..1122
Translation	MDTNKRILCRVGLDVLILLCAGFPILLFFLLGEPYKRGFFCDDESLKHPFHDSTVRNWMLYFIGAVIPVGVIFIVEVIISQNKAKQDNGNATSRRYVFMNYELPDWMIECYKKIGIYAFGAVLSQLTTDIAKYSIGRLRPHFIAVCQPQMADGSTCDDAINAGKYIQEFTCKGVGSSARMLKEMRLSFPSGHSSFTFFAMVYLALYLQARMTWRGSKLLRHLLQFLFIMVAWYTALSRVSDYKHHWSDVLAGSLIGSISALVVANYVSDLFQKPNTKPYLARTVQDMNASPAQAITITTN

Target ORF information:

RefSeq Version	NM_057845.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wunen (wun), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057845.5

ORF Insert Sequence:

ATGGATACCA ACAAAAGAAT CCTCTGCCGC GTGGGCTTGG ATGTTTTAAT CTTGCTCTGC 
GCTGGATTTC CCATACTGCT GTTTTTCCTG CTGGGCGAGC CGTACAAGCG GGGCTTCTTC 
TGCGATGACG AGTCACTGAA GCACCCGTTC CACGATTCTA CGGTCCGGAA TTGGATGCTA 
TACTTCATCG GAGCTGTGAT ACCAGTCGGC GTGATATTCA TCGTGGAGGT CATCATTTCA 
CAGAACAAGG CCAAGCAGGA TAATGGCAAT GCAACCAGTC GAAGGTACGT CTTTATGAAC 
TACGAACTGC CCGACTGGAT GATCGAGTGC TACAAGAAGA TCGGCATCTA TGCCTTCGGC 
GCTGTGCTCA GCCAGCTGAC CACGGATATA GCCAAGTACT CCATCGGCCG ACTGCGTCCC 
CATTTCATCG CGGTGTGCCA GCCTCAAATG GCCGACGGCA GCACCTGCGA CGATGCGATC 
AATGCGGGAA AATACATCCA GGAGTTCACA TGCAAGGGAG TTGGATCGTC GGCGCGCATG 
CTCAAAGAGA TGCGCCTCTC CTTCCCCAGT GGCCACTCCA GCTTCACCTT CTTCGCCATG 
GTCTATCTGG CGCTCTACCT GCAGGCTCGC ATGACCTGGC GGGGCTCCAA GCTGCTGCGT 
CACCTTCTCC AGTTCCTGTT CATCATGGTG GCCTGGTACA CAGCCCTAAG TCGCGTATCG 
GACTACAAGC ACCACTGGTC CGATGTGCTG GCAGGATCGC TTATTGGCTC CATAAGCGCA 
CTGGTCGTGG CCAACTATGT CTCCGACTTG TTTCAAAAGC CCAACACGAA GCCCTATTTG 
GCGCGTACAG TGCAAGACAT GAATGCATCG CCGGCCCAGG CCATAACGAT TACTACAAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wun cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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