VhaAC45 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	VhaAC45
Entrez Gene ID	35944
Full Name	Vacuolar H[+] ATPase AC45 accessory subunit
Synonyms	CG8029,Dmel\CG8029,anon-WO0118547.117,l(2)SH1634,l(2)SH2 1634,vhaAC45
General protein information	Preferred Names Vacuolar H[+] ATPase AC45 accessory subunit Names CG8029 gene product from transcript CG8029-RB CG8029-PA CG8029-PB VhaAC45-PA VhaAC45-PB lethal (2) SH1634 vacuolar H[+] ATPase AC45 accessory subunit vacuolar H[+] ATPase accessory protein AC45
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45B4-45B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_136626.5	NP_610470.1	vacuolar H[+] ATPase AC45 accessory subunit, isoform B
NM_165665.2	NP_724770.1	vacuolar H[+] ATPase AC45 accessory subunit, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003879	XP_310443.5
Drosophila melanogaster (fruit fly)	VhaAC45	NP_724770.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VhaAC45 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VhaAC45 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09362	NM_136626.5 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase AC45 accessory subunit, transcript variant B (VhaAC45), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09362	NM_165665.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase AC45 accessory subunit, transcript variant A (VhaAC45), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VhaAC45 ( NM_136626.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610470.1 Drosophila melanogaster vacuolar H[+] ATPase AC45 accessory subunit, transcript variant B (VhaAC45), mRNA.

Price & Availability↑

CloneID	OFa09362
Clone ID Related Accession (Same CDS sequence)	NM_136626.5 , NM_165665.2
Accession Version	NM_136626.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase AC45 accessory subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136626.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGTGGA AATCGCTGAT TGCGTTGTGC GTCATTGGGG CTGCCGTGGC GGAGCAAACG 
CCCGTCTTTT TGTGGGGAGC CAACAGTGTG GCGAAACCCT CCCTGAAGAC GGTGTCCCAA 
GTGGAGTTTG CCGAGCAGTT GGCTGCATTG CTGGAAGATC ACATGGTCGT GGCCTTCGAG 
GAAAATGGCC TGAGCAGCAA GGACTTCCTG TGCTCCAACT CCCAGGCGCA GTCCTGCTAC 
GCCCAGCTGC AGGGAGTGAG CCCCAAGACC TACTACACCA GCGTGGAGAA CCCCTCGGAG 
GCACTGCGCT CAGTGGCCGC CAAGCGCGAG CACAACTCCA TCGATGCCAG TGGAAAGCTG 
ACCACGCCGG CCAAGTGTGC CGTAGGTACG GCCCTGTTTG TGACCTTCGA GGACGCCGCC 
GAGAGCCGGG AGGCCAGTCT GGAGTCACAT GACGCCGCCA TAGCTGCTAT CAGCAAGCAG 
TTCGAGTGCA AGGTGGCTTA TCTGTACCTG GCCGCCCCCT CCACGGCTCC TGTGGTGCAG 
CGTCGTACCC GCCGTGACAC CGCCGCCACA ACCGGTGGCA TCATGTGGAA GTCGACCAAT 
CAGTTTCAGA TCTTCTACAC TGCCCTGCTC TACAACGGCA ACCCCATCAC CGTCACCGAC 
CTCAAGCTCA CCAACTCTAG CTCTACCAAG CTATCCGTTG TTATGGACAC ATCTGTCGCC 
GACAAGCCAA TCACCTTTGA CGTTGTTTAT AATGGCGGAT ACTTTAGTCT GAGCAATTTG 
GTCTACGACA ACAACAACTT CCGCTCCAGC GGCGTGAATG CCCCAACCAC GTTCTCTTAC 
TCGTGCGGCA ACCTCACCCT CGAGTCCGCG GCCGTCAACA ACATGTACAA CACCCTGAGC 
TTCAAGTCCC TGCAGCTGCA GGCTCCCTTC GATGGTACGT ACAAGGAGGA CTTTCCCTTC 
GGCGACTCCT GGGACTGTGT GGGCTTCGTG ACGCCCGGTA TCCTGATGGG ACTGTTTGTG 
GTCGCCCTAC TGCTGGTCAT CATGTTCGTC GGCGTCTGCT GGATGATGGA CATCAACACG 
ATGGACCGCT TCGACGATCC CAAGGGCAAG ACTATCACCA TCAATGCCGC CGCCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610470.1
CDS	117..1256
Translation	MLWKSLIALCVIGAAVAEQTPVFLWGANSVAKPSLKTVSQVEFAEQLAALLEDHMVVAFEENGLSSKDFLCSNSQAQSCYAQLQGVSPKTYYTSVENPSEALRSVAAKREHNSIDASGKLTTPAKCAVGTALFVTFEDAAESREASLESHDAAIAAISKQFECKVAYLYLAAPSTAPVVQRRTRRDTAATTGGIMWKSTNQFQIFYTALLYNGNPITVTDLKLTNSSSTKLSVVMDTSVADKPITFDVVYNGGYFSLSNLVYDNNNFRSSGVNAPTTFSYSCGNLTLESAAVNNMYNTLSFKSLQLQAPFDGTYKEDFPFGDSWDCVGFVTPGILMGLFVVALLLVIMFVGVCWMMDINTMDRFDDPKGKTITINAAAE

Target ORF information:

RefSeq Version	NM_136626.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase AC45 accessory subunit, transcript variant B (VhaAC45), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136626.5

ORF Insert Sequence:

ATGTTGTGGA AATCGCTGAT TGCGTTGTGC GTCATTGGGG CTGCCGTGGC GGAGCAAACG 
CCCGTCTTTT TGTGGGGAGC CAACAGTGTG GCGAAACCCT CCCTGAAGAC GGTGTCCCAA 
GTGGAGTTTG CCGAGCAGTT GGCTGCATTG CTGGAAGATC ACATGGTCGT GGCCTTCGAG 
GAAAATGGCC TGAGCAGCAA GGACTTCCTG TGCTCCAACT CCCAGGCGCA GTCCTGCTAC 
GCCCAGCTGC AGGGAGTGAG CCCCAAGACC TACTACACCA GCGTGGAGAA CCCCTCGGAG 
GCACTGCGCT CAGTGGCCGC CAAGCGCGAG CACAACTCCA TCGATGCCAG TGGAAAGCTG 
ACCACGCCGG CCAAGTGTGC CGTAGGTACG GCCCTGTTTG TGACCTTCGA GGACGCCGCC 
GAGAGCCGGG AGGCCAGTCT GGAGTCACAT GACGCCGCCA TAGCTGCTAT CAGCAAGCAG 
TTCGAGTGCA AGGTGGCTTA TCTGTACCTG GCCGCCCCCT CCACGGCTCC TGTGGTGCAG 
CGTCGTACCC GCCGTGACAC CGCCGCCACA ACCGGTGGCA TCATGTGGAA GTCGACCAAT 
CAGTTTCAGA TCTTCTACAC TGCCCTGCTC TACAACGGCA ACCCCATCAC CGTCACCGAC 
CTCAAGCTCA CCAACTCTAG CTCTACCAAG CTATCCGTTG TTATGGACAC ATCTGTCGCC 
GACAAGCCAA TCACCTTTGA CGTTGTTTAT AATGGCGGAT ACTTTAGTCT GAGCAATTTG 
GTCTACGACA ACAACAACTT CCGCTCCAGC GGCGTGAATG CCCCAACCAC GTTCTCTTAC 
TCGTGCGGCA ACCTCACCCT CGAGTCCGCG GCCGTCAACA ACATGTACAA CACCCTGAGC 
TTCAAGTCCC TGCAGCTGCA GGCTCCCTTC GATGGTACGT ACAAGGAGGA CTTTCCCTTC 
GGCGACTCCT GGGACTGTGT GGGCTTCGTG ACGCCCGGTA TCCTGATGGG ACTGTTTGTG 
GTCGCCCTAC TGCTGGTCAT CATGTTCGTC GGCGTCTGCT GGATGATGGA CATCAACACG 
ATGGACCGCT TCGACGATCC CAAGGGCAAG ACTATCACCA TCAATGCCGC CGCCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VhaAC45 ( NM_165665.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724770.1 Drosophila melanogaster vacuolar H[+] ATPase AC45 accessory subunit, transcript variant A (VhaAC45), mRNA.

Price & Availability↑

CloneID	OFa09362
Clone ID Related Accession (Same CDS sequence)	NM_136626.5 , NM_165665.2
Accession Version	NM_165665.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase AC45 accessory subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165665.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGTGGA AATCGCTGAT TGCGTTGTGC GTCATTGGGG CTGCCGTGGC GGAGCAAACG 
CCCGTCTTTT TGTGGGGAGC CAACAGTGTG GCGAAACCCT CCCTGAAGAC GGTGTCCCAA 
GTGGAGTTTG CCGAGCAGTT GGCTGCATTG CTGGAAGATC ACATGGTCGT GGCCTTCGAG 
GAAAATGGCC TGAGCAGCAA GGACTTCCTG TGCTCCAACT CCCAGGCGCA GTCCTGCTAC 
GCCCAGCTGC AGGGAGTGAG CCCCAAGACC TACTACACCA GCGTGGAGAA CCCCTCGGAG 
GCACTGCGCT CAGTGGCCGC CAAGCGCGAG CACAACTCCA TCGATGCCAG TGGAAAGCTG 
ACCACGCCGG CCAAGTGTGC CGTAGGTACG GCCCTGTTTG TGACCTTCGA GGACGCCGCC 
GAGAGCCGGG AGGCCAGTCT GGAGTCACAT GACGCCGCCA TAGCTGCTAT CAGCAAGCAG 
TTCGAGTGCA AGGTGGCTTA TCTGTACCTG GCCGCCCCCT CCACGGCTCC TGTGGTGCAG 
CGTCGTACCC GCCGTGACAC CGCCGCCACA ACCGGTGGCA TCATGTGGAA GTCGACCAAT 
CAGTTTCAGA TCTTCTACAC TGCCCTGCTC TACAACGGCA ACCCCATCAC CGTCACCGAC 
CTCAAGCTCA CCAACTCTAG CTCTACCAAG CTATCCGTTG TTATGGACAC ATCTGTCGCC 
GACAAGCCAA TCACCTTTGA CGTTGTTTAT AATGGCGGAT ACTTTAGTCT GAGCAATTTG 
GTCTACGACA ACAACAACTT CCGCTCCAGC GGCGTGAATG CCCCAACCAC GTTCTCTTAC 
TCGTGCGGCA ACCTCACCCT CGAGTCCGCG GCCGTCAACA ACATGTACAA CACCCTGAGC 
TTCAAGTCCC TGCAGCTGCA GGCTCCCTTC GATGGTACGT ACAAGGAGGA CTTTCCCTTC 
GGCGACTCCT GGGACTGTGT GGGCTTCGTG ACGCCCGGTA TCCTGATGGG ACTGTTTGTG 
GTCGCCCTAC TGCTGGTCAT CATGTTCGTC GGCGTCTGCT GGATGATGGA CATCAACACG 
ATGGACCGCT TCGACGATCC CAAGGGCAAG ACTATCACCA TCAATGCCGC CGCCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724770.1
CDS	205..1344
Translation	MLWKSLIALCVIGAAVAEQTPVFLWGANSVAKPSLKTVSQVEFAEQLAALLEDHMVVAFEENGLSSKDFLCSNSQAQSCYAQLQGVSPKTYYTSVENPSEALRSVAAKREHNSIDASGKLTTPAKCAVGTALFVTFEDAAESREASLESHDAAIAAISKQFECKVAYLYLAAPSTAPVVQRRTRRDTAATTGGIMWKSTNQFQIFYTALLYNGNPITVTDLKLTNSSSTKLSVVMDTSVADKPITFDVVYNGGYFSLSNLVYDNNNFRSSGVNAPTTFSYSCGNLTLESAAVNNMYNTLSFKSLQLQAPFDGTYKEDFPFGDSWDCVGFVTPGILMGLFVVALLLVIMFVGVCWMMDINTMDRFDDPKGKTITINAAAE

Target ORF information:

RefSeq Version	NM_165665.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase AC45 accessory subunit, transcript variant A (VhaAC45), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165665.2

ORF Insert Sequence:

ATGTTGTGGA AATCGCTGAT TGCGTTGTGC GTCATTGGGG CTGCCGTGGC GGAGCAAACG 
CCCGTCTTTT TGTGGGGAGC CAACAGTGTG GCGAAACCCT CCCTGAAGAC GGTGTCCCAA 
GTGGAGTTTG CCGAGCAGTT GGCTGCATTG CTGGAAGATC ACATGGTCGT GGCCTTCGAG 
GAAAATGGCC TGAGCAGCAA GGACTTCCTG TGCTCCAACT CCCAGGCGCA GTCCTGCTAC 
GCCCAGCTGC AGGGAGTGAG CCCCAAGACC TACTACACCA GCGTGGAGAA CCCCTCGGAG 
GCACTGCGCT CAGTGGCCGC CAAGCGCGAG CACAACTCCA TCGATGCCAG TGGAAAGCTG 
ACCACGCCGG CCAAGTGTGC CGTAGGTACG GCCCTGTTTG TGACCTTCGA GGACGCCGCC 
GAGAGCCGGG AGGCCAGTCT GGAGTCACAT GACGCCGCCA TAGCTGCTAT CAGCAAGCAG 
TTCGAGTGCA AGGTGGCTTA TCTGTACCTG GCCGCCCCCT CCACGGCTCC TGTGGTGCAG 
CGTCGTACCC GCCGTGACAC CGCCGCCACA ACCGGTGGCA TCATGTGGAA GTCGACCAAT 
CAGTTTCAGA TCTTCTACAC TGCCCTGCTC TACAACGGCA ACCCCATCAC CGTCACCGAC 
CTCAAGCTCA CCAACTCTAG CTCTACCAAG CTATCCGTTG TTATGGACAC ATCTGTCGCC 
GACAAGCCAA TCACCTTTGA CGTTGTTTAT AATGGCGGAT ACTTTAGTCT GAGCAATTTG 
GTCTACGACA ACAACAACTT CCGCTCCAGC GGCGTGAATG CCCCAACCAC GTTCTCTTAC 
TCGTGCGGCA ACCTCACCCT CGAGTCCGCG GCCGTCAACA ACATGTACAA CACCCTGAGC 
TTCAAGTCCC TGCAGCTGCA GGCTCCCTTC GATGGTACGT ACAAGGAGGA CTTTCCCTTC 
GGCGACTCCT GGGACTGTGT GGGCTTCGTG ACGCCCGGTA TCCTGATGGG ACTGTTTGTG 
GTCGCCCTAC TGCTGGTCAT CATGTTCGTC GGCGTCTGCT GGATGATGGA CATCAACACG 
ATGGACCGCT TCGACGATCC CAAGGGCAAG ACTATCACCA TCAATGCCGC CGCCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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VhaAC45 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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