shrb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	shrb
Entrez Gene ID	35933
Full Name	shrub
Synonyms	CG8055,DmSNF2,Dmel\CG8055,Dvps32,ESCRT,ESCRT-III,Shrb,Shrub,Snf7,Snf7/Vps32,Vps32,anon-WO0118547.180,vps32,vps32/snf7
General protein information	Preferred Names shrub Names CG8055 gene product from transcript CG8055-RA CG8055-PA Vps32 shrb-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45A12-45A12

mRNA and Protein(s)

mRNA	Protein	Name
NM_136618.5	NP_610462.3	shrub

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CHMP4B	XP_542966.1
Saccharomyces cerevisiae (baker's yeast)	SNF7	NP_013125.1
Eremothecium gossypii	AGOS_AFR209W	NP_985756.1
Arabidopsis thaliana (thale cress)	SNF7.2	NP_179573.1
Bos taurus (cattle)	CHMP4B	NP_001121978.1
Mus musculus (house mouse)	Chmp4b	NP_083638.1
Danio rerio (zebrafish)	zgc:56112	NP_956489.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005316	XP_315330.3
Neurospora crassa	NCU01282	XP_960819.1
Arabidopsis thaliana (thale cress)	SNF7.1	NP_001078468.1
Xenopus tropicalis (tropical clawed frog)	chmp4b	NP_001004866.1
Schizosaccharomyces pombe (fission yeast)	vps32	NP_594271.1
Homo sapiens (human)	CHMP4B	NP_789782.1
	LOC709004	XP_001105255.1
Drosophila melanogaster (fruit fly)	shrb	NP_610462.3
Oryza sativa (rice)	Os09g0267600	NP_001062723.1
Pan troglodytes (chimpanzee)	CHMP4B	XP_003316914.2
Rattus norvegicus (Norway rat)	Chmp4bl1	NP_001263385.1
Gallus gallus (chicken)	CHMP4B	NP_001006286.1
Kluyveromyces lactis	KLLA0F17820g	XP_455879.1
Magnaporthe oryzae (rice blast fungus)	MGG_04095	XP_003719742.1
Oryza sativa (rice)	Os06g0608500	NP_001058040.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we found that Lethal giant discs (lgd), known to be required for Shrub function in the endosomal pathway, also regulates the duration of abscission in GSCs.
Title: Abscission is regulated by the ESCRT-III protein shrub in Drosophila germline stem cells.

Mechanistically, we show that the direct interaction between ALIX and Shrub is required to ensure cytokinesis completion with normal kinetics in female germline stem cell .
Title: ALIX and ESCRT-III coordinately control cytokinetic abscission during germline stem cell division in vivo.

Borr interacts and colocalizes with the ESCRT-III component Shrb in Drosophila cells.
Title: The chromosomal passenger complex controls the function of endosomal sorting complex required for transport-III Snf7 proteins during cytokinesis.

genetic studies indicate that the activation of Notch in ESCRT and lgd mutant cells occurs in a different manner and that the activity of Shrub and other ESCRT components are required for the activation of Notch in lgd mutant cells
Title: The tumour suppressor Lethal (2) giant discs is required for the function of the ESCRT-III component Shrub/CHMP4.

It was shown that Shrub plays a pivotal rate-limiting step in late endosomal ligand-independent Notch activation, depending on the Deltex-dependent ubiquitinylation state of the receptor.
Title: Synergy between the ESCRT-III complex and Deltex defines a ligand-independent Notch signal.

The following shrb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the shrb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09345	NM_136618.5 Latest version!	Drosophila melanogaster shrub (shrb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

shrb ( NM_136618.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610462.3 Drosophila melanogaster shrub (shrb), mRNA.

Price & Availability↑

CloneID	OFa09345
Clone ID Related Accession (Same CDS sequence)	NM_136618.5
Accession Version	NM_136618.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shrub
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136618.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTTCT TCGGGAAGAT GTTCGGCGGC AAGAAGGAAG TGGCCCCCAC CACCGGCGAG 
GCGATACAGA AGCTGCGCGA GACGGAGAAC ATGCTTATCA AAAAGCAGGA GTTCCTGGAG 
GCCAAGATCG AGGACGAACT GAATATAGCC CGCAAGAATG CGTCTAAAAA CAAAAGAGTG 
GCCCTGCAAG CACTCAAGAA AAAGAAGCGG CTGGAGAAGC AACTCCAGCA GATCGATGGC 
ACCCTGTCCA CAATCGAAAT GCAGCGCGAG GCTCTGGAGA GCGCCAACAC AAACACTGCC 
GTCTTGACCA CGATGAAGAA TGCCGCGGAT GCCCTCAAGA GAGCCCATCA GAATATGGAC 
GTGGACAAGG TGCACGACAT GATGGATGAC ATTGCCGAGC AGCAGGACGT GGCCCGCGAG 
ATTTCCGATG CCATTTCGAA CCCAGTGGCA TTCGGCGCCG ATCTGGATGA TGAGGATCTC 
GAGCGGGAAC TCGACGAGCT GGAGCAGGAG AACTTTGATA AGGAAATCAT TGGCATACCC 
GAGCCGACGC CCACATTGCC AGAAGCACCC ACAGAAGATT TGCCCGAGAA GGCCAAAGAA 
AAGAAAAAGG CGACAACCAC GACTGCCGTG GAGGATGATG ATGATCCAGA CATGAAGCAG 
CTTTTATCCT GGTCCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610462.3
CDS	118..798
Translation	MSFFGKMFGGKKEVAPTTGEAIQKLRETENMLIKKQEFLEAKIEDELNIARKNASKNKRVALQALKKKKRLEKQLQQIDGTLSTIEMQREALESANTNTAVLTTMKNAADALKRAHQNMDVDKVHDMMDDIAEQQDVAREISDAISNPVAFGADLDDEDLERELDELEQENFDKEIIGIPEPTPTLPEAPTEDLPEKAKEKKKATTTTAVEDDDDPDMKQLLSWSN

Target ORF information:

RefSeq Version	NM_136618.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shrub (shrb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136618.5

ORF Insert Sequence:

ATGAGTTTCT TCGGGAAGAT GTTCGGCGGC AAGAAGGAAG TGGCCCCCAC CACCGGCGAG 
GCGATACAGA AGCTGCGCGA GACGGAGAAC ATGCTTATCA AAAAGCAGGA GTTCCTGGAG 
GCCAAGATCG AGGACGAACT GAATATAGCC CGCAAGAATG CGTCTAAAAA CAAAAGAGTG 
GCCCTGCAAG CACTCAAGAA AAAGAAGCGG CTGGAGAAGC AACTCCAGCA GATCGATGGC 
ACCCTGTCCA CAATCGAAAT GCAGCGCGAG GCTCTGGAGA GCGCCAACAC AAACACTGCC 
GTCTTGACCA CGATGAAGAA TGCCGCGGAT GCCCTCAAGA GAGCCCATCA GAATATGGAC 
GTGGACAAGG TGCACGACAT GATGGATGAC ATTGCCGAGC AGCAGGACGT GGCCCGCGAG 
ATTTCCGATG CCATTTCGAA CCCAGTGGCA TTCGGCGCCG ATCTGGATGA TGAGGATCTC 
GAGCGGGAAC TCGACGAGCT GGAGCAGGAG AACTTTGATA AGGAAATCAT TGGCATACCC 
GAGCCGACGC CCACATTGCC AGAAGCACCC ACAGAAGATT TGCCCGAGAA GGCCAAAGAA 
AAGAAAAAGG CGACAACCAC GACTGCCGTG GAGGATGATG ATGATCCAGA CATGAAGCAG 
CTTTTATCCT GGTCCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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shrb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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