CG8170 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8170
Entrez Gene ID	35908
Full Name	CG8170 gene product from transcript CG8170-RA
Synonyms	Dmel\CG8170,SP54
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45A1-45A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043068.2	NP_001036533.1	uncharacterized protein, isoform B
NM_136597.4	NP_610441.2	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRSS22	NP_071402.1
Mus musculus (house mouse)	Prss22	NP_598492.2
Pan troglodytes (chimpanzee)	PRSS22	XP_510756.3
Rattus norvegicus (Norway rat)	Prss22	NP_001100454.1
Drosophila melanogaster (fruit fly)	CG8170	NP_001036533.1
Canis lupus familiaris (dog)	PRSS22	XP_005622142.1
Bos taurus (cattle)	PRSS22	XP_002697973.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG8170 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8170 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09309	NM_001043068.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8170), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09310	NM_136597.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8170), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8170 ( NM_001043068.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036533.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG8170), mRNA.

Price & Availability↑

CloneID	OFa09309
Clone ID Related Accession (Same CDS sequence)	NM_001043068.2
Accession Version	NM_001043068.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001043068.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAGTGG CCACCGACGA AAACAATACC AATACCACCA CCGCCAGCCG GAGCACACGC 
AACACCTGGA GATGGAGCCG CCTGGCGTTG GCCGTTAGTC TCTTCGCCTA CTGCTGCTGC 
GCCTCATCCG CGGCCACGCC CACCACCTCG GTGACCACGG CCGCCCTTCT CAGCGGCCAC 
CAGCTGCTGC CCTGGCAAAC GGCGGCTGCG ACGGCGGCCA CCACGCCCTC CACCGCCCGC 
AATCTGTATG CGCCACACCA GAGCTTCAGC CGACACGATG ACATCAGCTT TAAGGATCTG 
CGCCAGCGCA GCGATGGCAC GGTGGTGCAG TCCTTTGGTT CCTATCGCAC CGCTCGTCAG 
TTTGGTGGCT ACTCAACCAC TGCCCAGCGG CGATCGGACC CCGCCGTAGA AATCATTCCA 
GCTCCGTCCG TGGAACTGCC GCAGGGTGAG CTGATCACGG TGCCCAAGCA GCTGGTGCCC 
ACGATGCCGG AGAATGTGAC TCGCCAGGGT CGGCGTCGCA ACTACATGTA CATACCGCTG 
CAGACGGAGG GGGAGAATGG ATCCGCGGCG CTGCTACAAC TGCGTCCCAG TCGGAACGCC 
ACGCTTTCCG CGCTGGGACC TCCGGCCGAT GGAGTGGCCG CAGTGGCCCA TGAATTTGGC 
GCCGATCTGG CCACCCAGGG ATCTGGACCG GAGCAGCCAC CGCTGGCATT GGAACTGGGT 
CCCGAGCGAA CCGAACGCAG CGATCTTATG GCCGCCGCCA GCTCCACGGA CAAGCTGGAG 
TACGCCGAGC CGGAGAACAC CAGTCGCCGT GTGGGCTTCC AATTTCCCAG CTACAGCCTA 
AAGCCCGCCA GTCCCTTCCA TCCGCAGGCC TACCGCGAGG ATAACCCCAT ATTTGGGGAC 
ACCAGTGCCG GAGGCTATGA GCCCCAGCCG TACAGCGAAG ATGCGTCTCC CGTTCCGCCA 
CCCACGCAGT ACGAGCAGCA CGAGACGCGG CACACACGGC AGCTCTACTT TGATTCGGAC 
ACCGCGTCCT CCAGCTTCGA GTTTCCCGGC CTGGTGAGCA ACTCCAAGCC GCATGGCCTC 
AAGCTCTATC GCGACGATGT GACCAAGTTC GGGGATATCA ATGGACCGAT CACCGCGCTA 
CCGCAGCAGC GACCCCAGCG AGGCTTCTAC TTCGGCGACA CTGAGTTCCG CACTGGTCCT 
CCTGCTCCGG TCCGCCAGTT TGGGCCGCAA AAGAACTTCC AGGAGTACGT GGGACCCAGC 
GAGTACCAGG GTACCCGGAA GAGCCGCTAC TATCCCTACA AGTCGTCGCG CAGTCCGAGG 
GTCGTCTTCC CCACCAACGA CAACGTGGGC ACCACCGGAC CCAGTGGACC CGCCGGCAGC 
AGCGGACCCT CCGGAAACGG AGTCTACTTC AGCGATAACA TTGCTTTCAG GGACCAGAAC 
TTCGGCATCA ACGAATTGGC CGCCGTTCAG GATGTGCGCA ATGACTACAG TCTGCAGGAT 
CTGGACAGCG CCTCGGAGGC GACTAGCAGT CCGCAGTCGG CGAGCACGTT CAAGGAGAAA 
GGCTGCGGCA TTTCGCTGGC CAAGCAGACC GCCCAGCGGC GCATTGTGGG CGGCGATGAT 
GCGGGCTTCG GCTCCTTTCC CTGGCAGGCC TACATCCGCA TCGGTTCCTC CAGGTGCGGA 
GGTTCGTTGA TCTCGCGACG ACACGTGGTC ACCGCTGGTC ATTGTGTGGC CCGGGCCACT 
CCGCGACAGG TGCATGTCAC GCTGGGTGAT TACGTGATCA ACTCGGCCGT GGAGCCACTG 
CCGGCCTACA CCTTCGGAGT CCGCCGGATC GACGTGCATC CGTACTTCAA GTTCACGCCG 
CAGGCGGACC GCTTCGACAT CTCCGTGCTG ACGCTAGAGC GTACCGTGCA CTTCATGCCC 
CACATAGCTC CGATTTGCCT GCCCGAGAAG AACGAGGACT TCCTGGGCAA ATTTGGCTGG 
GCAGCCGGCT GGGGAGCTCT GAACCCGGGC TCCCGGCTGC GTCCCAAGAC GCTGCAGGCC 
GTGGATGTGC CCGTGATCGA GAACCGAATC TGCGAGCGCT GGCACCGGCA GAACGGCATC 
AACGTGGTCA TCTACCAGGA GATGCTCTGC GCGGGCTATC GCAACGGTGG CAAGGACTCG 
TGCCAAGGCG ACTCTGGAGG ACCTTTGATG CACGACAAGA ATGGGCGCTG GTACCTGATT 
GGTGTGGTCT CTGCTGGTTA CTCCTGCGCG TCCCGCGGTC AGCCGGGAAT CTATCACAGC 
GTCAGCAAGA CCGTGGACTG GGTATCCTAC GTCGTCGGAC TCACGATGAA CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036533.1
CDS	935..3271
Translation	MLVATDENNTNTTTASRSTRNTWRWSRLALAVSLFAYCCCASSAATPTTSVTTAALLSGHQLLPWQTAAATAATTPSTARNLYAPHQSFSRHDDISFKDLRQRSDGTVVQSFGSYRTARQFGGYSTTAQRRSDPAVEIIPAPSVELPQGELITVPKQLVPTMPENVTRQGRRRNYMYIPLQTEGENGSAALLQLRPSRNATLSALGPPADGVAAVAHEFGADLATQGSGPEQPPLALELGPERTERSDLMAAASSTDKLEYAEPENTSRRVGFQFPSYSLKPASPFHPQAYREDNPIFGDTSAGGYEPQPYSEDASPVPPPTQYEQHETRHTRQLYFDSDTASSSFEFPGLVSNSKPHGLKLYRDDVTKFGDINGPITALPQQRPQRGFYFGDTEFRTGPPAPVRQFGPQKNFQEYVGPSEYQGTRKSRYYPYKSSRSPRVVFPTNDNVGTTGPSGPAGSSGPSGNGVYFSDNIAFRDQNFGINELAAVQDVRNDYSLQDLDSASEATSSPQSASTFKEKGCGISLAKQTAQRRIVGGDDAGFGSFPWQAYIRIGSSRCGGSLISRRHVVTAGHCVARATPRQVHVTLGDYVINSAVEPLPAYTFGVRRIDVHPYFKFTPQADRFDISVLTLERTVHFMPHIAPICLPEKNEDFLGKFGWAAGWGALNPGSRLRPKTLQAVDVPVIENRICERWHRQNGINVVIYQEMLCAGYRNGGKDSCQGDSGGPLMHDKNGRWYLIGVVSAGYSCASRGQPGIYHSVSKTVDWVSYVVGLTMNI

Target ORF information:

RefSeq Version	NM_001043068.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8170), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043068.2

ORF Insert Sequence:

ATGTTAGTGG CCACCGACGA AAACAATACC AATACCACCA CCGCCAGCCG GAGCACACGC 
AACACCTGGA GATGGAGCCG CCTGGCGTTG GCCGTTAGTC TCTTCGCCTA CTGCTGCTGC 
GCCTCATCCG CGGCCACGCC CACCACCTCG GTGACCACGG CCGCCCTTCT CAGCGGCCAC 
CAGCTGCTGC CCTGGCAAAC GGCGGCTGCG ACGGCGGCCA CCACGCCCTC CACCGCCCGC 
AATCTGTATG CGCCACACCA GAGCTTCAGC CGACACGATG ACATCAGCTT TAAGGATCTG 
CGCCAGCGCA GCGATGGCAC GGTGGTGCAG TCCTTTGGTT CCTATCGCAC CGCTCGTCAG 
TTTGGTGGCT ACTCAACCAC TGCCCAGCGG CGATCGGACC CCGCCGTAGA AATCATTCCA 
GCTCCGTCCG TGGAACTGCC GCAGGGTGAG CTGATCACGG TGCCCAAGCA GCTGGTGCCC 
ACGATGCCGG AGAATGTGAC TCGCCAGGGT CGGCGTCGCA ACTACATGTA CATACCGCTG 
CAGACGGAGG GGGAGAATGG ATCCGCGGCG CTGCTACAAC TGCGTCCCAG TCGGAACGCC 
ACGCTTTCCG CGCTGGGACC TCCGGCCGAT GGAGTGGCCG CAGTGGCCCA TGAATTTGGC 
GCCGATCTGG CCACCCAGGG ATCTGGACCG GAGCAGCCAC CGCTGGCATT GGAACTGGGT 
CCCGAGCGAA CCGAACGCAG CGATCTTATG GCCGCCGCCA GCTCCACGGA CAAGCTGGAG 
TACGCCGAGC CGGAGAACAC CAGTCGCCGT GTGGGCTTCC AATTTCCCAG CTACAGCCTA 
AAGCCCGCCA GTCCCTTCCA TCCGCAGGCC TACCGCGAGG ATAACCCCAT ATTTGGGGAC 
ACCAGTGCCG GAGGCTATGA GCCCCAGCCG TACAGCGAAG ATGCGTCTCC CGTTCCGCCA 
CCCACGCAGT ACGAGCAGCA CGAGACGCGG CACACACGGC AGCTCTACTT TGATTCGGAC 
ACCGCGTCCT CCAGCTTCGA GTTTCCCGGC CTGGTGAGCA ACTCCAAGCC GCATGGCCTC 
AAGCTCTATC GCGACGATGT GACCAAGTTC GGGGATATCA ATGGACCGAT CACCGCGCTA 
CCGCAGCAGC GACCCCAGCG AGGCTTCTAC TTCGGCGACA CTGAGTTCCG CACTGGTCCT 
CCTGCTCCGG TCCGCCAGTT TGGGCCGCAA AAGAACTTCC AGGAGTACGT GGGACCCAGC 
GAGTACCAGG GTACCCGGAA GAGCCGCTAC TATCCCTACA AGTCGTCGCG CAGTCCGAGG 
GTCGTCTTCC CCACCAACGA CAACGTGGGC ACCACCGGAC CCAGTGGACC CGCCGGCAGC 
AGCGGACCCT CCGGAAACGG AGTCTACTTC AGCGATAACA TTGCTTTCAG GGACCAGAAC 
TTCGGCATCA ACGAATTGGC CGCCGTTCAG GATGTGCGCA ATGACTACAG TCTGCAGGAT 
CTGGACAGCG CCTCGGAGGC GACTAGCAGT CCGCAGTCGG CGAGCACGTT CAAGGAGAAA 
GGCTGCGGCA TTTCGCTGGC CAAGCAGACC GCCCAGCGGC GCATTGTGGG CGGCGATGAT 
GCGGGCTTCG GCTCCTTTCC CTGGCAGGCC TACATCCGCA TCGGTTCCTC CAGGTGCGGA 
GGTTCGTTGA TCTCGCGACG ACACGTGGTC ACCGCTGGTC ATTGTGTGGC CCGGGCCACT 
CCGCGACAGG TGCATGTCAC GCTGGGTGAT TACGTGATCA ACTCGGCCGT GGAGCCACTG 
CCGGCCTACA CCTTCGGAGT CCGCCGGATC GACGTGCATC CGTACTTCAA GTTCACGCCG 
CAGGCGGACC GCTTCGACAT CTCCGTGCTG ACGCTAGAGC GTACCGTGCA CTTCATGCCC 
CACATAGCTC CGATTTGCCT GCCCGAGAAG AACGAGGACT TCCTGGGCAA ATTTGGCTGG 
GCAGCCGGCT GGGGAGCTCT GAACCCGGGC TCCCGGCTGC GTCCCAAGAC GCTGCAGGCC 
GTGGATGTGC CCGTGATCGA GAACCGAATC TGCGAGCGCT GGCACCGGCA GAACGGCATC 
AACGTGGTCA TCTACCAGGA GATGCTCTGC GCGGGCTATC GCAACGGTGG CAAGGACTCG 
TGCCAAGGCG ACTCTGGAGG ACCTTTGATG CACGACAAGA ATGGGCGCTG GTACCTGATT 
GGTGTGGTCT CTGCTGGTTA CTCCTGCGCG TCCCGCGGTC AGCCGGGAAT CTATCACAGC 
GTCAGCAAGA CCGTGGACTG GGTATCCTAC GTCGTCGGAC TCACGATGAA CATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8170 ( NM_136597.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610441.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG8170), mRNA.

Price & Availability↑

CloneID	OFa09310
Clone ID Related Accession (Same CDS sequence)	NM_136597.4
Accession Version	NM_136597.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136597.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAGTGG CCACCGACGA AAACAATACC AATACCACCA CCGCCAGCCG GAGCACACGC 
AACACCTGGA GATGGAGCCG CCTGGCGTTG GCCGTTAGTC TCTTCGCCTA CTGCTGCTGC 
GCCTCATCCG CGGCCACGCC CACCACCTCG GTGACCACGG CCGCCCTTCT CAGCGGCCAC 
CAGCTGCTGC CCTGGCAAAC GGCGGCTGCG ACGGCGGCCA CCACGCCCTC CACCGCCCGC 
AATCTGTATG CGCCACACCA GAGCTTCAGC CGACACGATG ACATCAGCTT TAAGGATCTG 
CGCCAGCGCA GCGATGGCAC GGTGGTGCAG TCCTTTGGTT CCTATCGCAC CGCTCGTCAG 
TTTGGTGGCT ACTCAACCAC TGCCCAGCGG CGATCGGACC CCGCCGTAGA AATCATTCCA 
GCTCCGTCCG TGGAACTGCC GCAGGGTGAG CTGATCACGG TGCCCAAGCA GCTGGTGCCC 
ACGATGCCGG AGAATGTGAC TCGCCAGGGT CGGCGTCGCA ACTACATGTA CATACCGCTG 
CAGACGGAGG GGGAGAATGG ATCCGCGGCG CTGCTACAAC TGCGTCCCAG TCGGAACGCC 
ACGCTTTCCG CGCTGGGACC TCCGGCCGAT GGAGTGGCCG CAGTGGCCCA TGAATTTGGC 
GCCGATCTGG CCACCCAGGG ATCTGGACCG GAGCAGCCAC CGCTGGCATT GGAACTGGGT 
CCCGAGCGAA CCGAACGCAG CGATCTTATG GCCGCCGCCA GCTCCACGGA CAAGCTGGAG 
TACGCCGAGC CGGAGAACAC CAGTCGCCGT GTGGGCTTCC AATTTCCCAG CTACAGCCTA 
AAGCCCGCCA GTCCCTTCCA TCCGCAGGCC TACCGCGAGG ATAACCCCAT ATTTGGGGAC 
ACCAGTGCCG GAGGCTATGA GCCCCAGCCG TACAGCGAAG ATGCGTCTCC CGTTCCGCCA 
CCCACGCAGT ACGAGCAGCA CGAGACGCGG CACACACGGC AGCTCTACTT TGATTCGGAC 
ACCGCGTCCT CCAGCTTCGA GTTTCCCGGC CTGGTGAGCA ACTCCAAGCC GCATGGCCTC 
AAGCTCTATC GCGACGATGT GACCAAGTTC GGGGATATCA ATGGACCGAT CACCGCGCTA 
CCGCAGCAGC GACCCCAGCG AGGCTTCTAC TTCGGCGACA CTGAGTTCCG CACTGGTCCT 
CCTGCTCCGG TCCGCCAGTT TGGGCCGCAA AAGAACTTCC AGGAGTACGT GGGACCCAGC 
GAGTACCAGG GTACCCGGAA GAGCCGCTAC TATCCCTACA AGTCGTCGCG CAGTCCGAGG 
GTCGTCTTCC CCACCAACGA CAACGTGGGC ACCACCGGAC CCAGTGGACC CGCCGGCAGC 
AGCGGACCCT CCGGAAACGG AGTCTACTTC AGCGATAACA TTGCTTTCAG GGACCAGAAC 
TTCGGCATCA ACGAATTGGC CGCCGTTCAG GATGTGCGCA ATGACTACAG TCTGCAGGAT 
CTGGACAGCG CCTCGGAGGC GACTAGCAGT CCGCAGTCGG CGAGCACGTT CAAGGAGAAA 
GTCGACATCA CAACGGACAC GGAGTGCCAG CACCGGGGCG GCACGTGCGA GTTCTTCCTG 
GGCTGCTGGC TCTCCGGCGG CCTCATTCAG GGCACCTGCG ACGGACTGCT GCGCGGCTGT 
TGCCACCGCA CGGCCAAGTC GGCCAATCTT GGCAGCTCGG ACTTCGTCGG CAATGCCGTA 
GATCTCACTG ATCTGCCGCA AAAGAACTAC GGACCTGTGA ACAACGAACC CAGCTGCGGC 
ATTTCGCTGG CCAAGCAGAC CGCCCAGCGG CGCATTGTGG GCGGCGATGA TGCGGGCTTC 
GGCTCCTTTC CCTGGCAGGC CTACATCCGC ATCGGTTCCT CCAGGTGCGG AGGTTCGTTG 
ATCTCGCGAC GACACGTGGT CACCGCTGGT CATTGTGTGG CCCGGGCCAC TCCGCGACAG 
GTGCATGTCA CGCTGGGTGA TTACGTGATC AACTCGGCCG TGGAGCCACT GCCGGCCTAC 
ACCTTCGGAG TCCGCCGGAT CGACGTGCAT CCGTACTTCA AGTTCACGCC GCAGGCGGAC 
CGCTTCGACA TCTCCGTGCT GACGCTAGAG CGTACCGTGC ACTTCATGCC CCACATAGCT 
CCGATTTGCC TGCCCGAGAA GAACGAGGAC TTCCTGGGCA AATTTGGCTG GGCAGCCGGC 
TGGGGAGCTC TGAACCCGGG CTCCCGGCTG CGTCCCAAGA CGCTGCAGGC CGTGGATGTG 
CCCGTGATCG AGAACCGAAT CTGCGAGCGC TGGCACCGGC AGAACGGCAT CAACGTGGTC 
ATCTACCAGG AGATGCTCTG CGCGGGCTAT CGCAACGGTG GCAAGGACTC GTGCCAAGGC 
GACTCTGGAG GACCTTTGAT GCACGACAAG AATGGGCGCT GGTACCTGAT TGGTGTGGTC 
TCTGCTGGTT ACTCCTGCGC GTCCCGCGGT CAGCCGGGAA TCTATCACAG CGTCAGCAAG 
ACCGTGGACT GGGTATCCTA CGTCGTCGGA CTCACGATGA ACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610441.2
CDS	919..3486
Translation	MLVATDENNTNTTTASRSTRNTWRWSRLALAVSLFAYCCCASSAATPTTSVTTAALLSGHQLLPWQTAAATAATTPSTARNLYAPHQSFSRHDDISFKDLRQRSDGTVVQSFGSYRTARQFGGYSTTAQRRSDPAVEIIPAPSVELPQGELITVPKQLVPTMPENVTRQGRRRNYMYIPLQTEGENGSAALLQLRPSRNATLSALGPPADGVAAVAHEFGADLATQGSGPEQPPLALELGPERTERSDLMAAASSTDKLEYAEPENTSRRVGFQFPSYSLKPASPFHPQAYREDNPIFGDTSAGGYEPQPYSEDASPVPPPTQYEQHETRHTRQLYFDSDTASSSFEFPGLVSNSKPHGLKLYRDDVTKFGDINGPITALPQQRPQRGFYFGDTEFRTGPPAPVRQFGPQKNFQEYVGPSEYQGTRKSRYYPYKSSRSPRVVFPTNDNVGTTGPSGPAGSSGPSGNGVYFSDNIAFRDQNFGINELAAVQDVRNDYSLQDLDSASEATSSPQSASTFKEKVDITTDTECQHRGGTCEFFLGCWLSGGLIQGTCDGLLRGCCHRTAKSANLGSSDFVGNAVDLTDLPQKNYGPVNNEPSCGISLAKQTAQRRIVGGDDAGFGSFPWQAYIRIGSSRCGGSLISRRHVVTAGHCVARATPRQVHVTLGDYVINSAVEPLPAYTFGVRRIDVHPYFKFTPQADRFDISVLTLERTVHFMPHIAPICLPEKNEDFLGKFGWAAGWGALNPGSRLRPKTLQAVDVPVIENRICERWHRQNGINVVIYQEMLCAGYRNGGKDSCQGDSGGPLMHDKNGRWYLIGVVSAGYSCASRGQPGIYHSVSKTVDWVSYVVGLTMNI

Target ORF information:

RefSeq Version	NM_136597.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8170), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136597.4

ORF Insert Sequence:

ATGTTAGTGG CCACCGACGA AAACAATACC AATACCACCA CCGCCAGCCG GAGCACACGC 
AACACCTGGA GATGGAGCCG CCTGGCGTTG GCCGTTAGTC TCTTCGCCTA CTGCTGCTGC 
GCCTCATCCG CGGCCACGCC CACCACCTCG GTGACCACGG CCGCCCTTCT CAGCGGCCAC 
CAGCTGCTGC CCTGGCAAAC GGCGGCTGCG ACGGCGGCCA CCACGCCCTC CACCGCCCGC 
AATCTGTATG CGCCACACCA GAGCTTCAGC CGACACGATG ACATCAGCTT TAAGGATCTG 
CGCCAGCGCA GCGATGGCAC GGTGGTGCAG TCCTTTGGTT CCTATCGCAC CGCTCGTCAG 
TTTGGTGGCT ACTCAACCAC TGCCCAGCGG CGATCGGACC CCGCCGTAGA AATCATTCCA 
GCTCCGTCCG TGGAACTGCC GCAGGGTGAG CTGATCACGG TGCCCAAGCA GCTGGTGCCC 
ACGATGCCGG AGAATGTGAC TCGCCAGGGT CGGCGTCGCA ACTACATGTA CATACCGCTG 
CAGACGGAGG GGGAGAATGG ATCCGCGGCG CTGCTACAAC TGCGTCCCAG TCGGAACGCC 
ACGCTTTCCG CGCTGGGACC TCCGGCCGAT GGAGTGGCCG CAGTGGCCCA TGAATTTGGC 
GCCGATCTGG CCACCCAGGG ATCTGGACCG GAGCAGCCAC CGCTGGCATT GGAACTGGGT 
CCCGAGCGAA CCGAACGCAG CGATCTTATG GCCGCCGCCA GCTCCACGGA CAAGCTGGAG 
TACGCCGAGC CGGAGAACAC CAGTCGCCGT GTGGGCTTCC AATTTCCCAG CTACAGCCTA 
AAGCCCGCCA GTCCCTTCCA TCCGCAGGCC TACCGCGAGG ATAACCCCAT ATTTGGGGAC 
ACCAGTGCCG GAGGCTATGA GCCCCAGCCG TACAGCGAAG ATGCGTCTCC CGTTCCGCCA 
CCCACGCAGT ACGAGCAGCA CGAGACGCGG CACACACGGC AGCTCTACTT TGATTCGGAC 
ACCGCGTCCT CCAGCTTCGA GTTTCCCGGC CTGGTGAGCA ACTCCAAGCC GCATGGCCTC 
AAGCTCTATC GCGACGATGT GACCAAGTTC GGGGATATCA ATGGACCGAT CACCGCGCTA 
CCGCAGCAGC GACCCCAGCG AGGCTTCTAC TTCGGCGACA CTGAGTTCCG CACTGGTCCT 
CCTGCTCCGG TCCGCCAGTT TGGGCCGCAA AAGAACTTCC AGGAGTACGT GGGACCCAGC 
GAGTACCAGG GTACCCGGAA GAGCCGCTAC TATCCCTACA AGTCGTCGCG CAGTCCGAGG 
GTCGTCTTCC CCACCAACGA CAACGTGGGC ACCACCGGAC CCAGTGGACC CGCCGGCAGC 
AGCGGACCCT CCGGAAACGG AGTCTACTTC AGCGATAACA TTGCTTTCAG GGACCAGAAC 
TTCGGCATCA ACGAATTGGC CGCCGTTCAG GATGTGCGCA ATGACTACAG TCTGCAGGAT 
CTGGACAGCG CCTCGGAGGC GACTAGCAGT CCGCAGTCGG CGAGCACGTT CAAGGAGAAA 
GTCGACATCA CAACGGACAC GGAGTGCCAG CACCGGGGCG GCACGTGCGA GTTCTTCCTG 
GGCTGCTGGC TCTCCGGCGG CCTCATTCAG GGCACCTGCG ACGGACTGCT GCGCGGCTGT 
TGCCACCGCA CGGCCAAGTC GGCCAATCTT GGCAGCTCGG ACTTCGTCGG CAATGCCGTA 
GATCTCACTG ATCTGCCGCA AAAGAACTAC GGACCTGTGA ACAACGAACC CAGCTGCGGC 
ATTTCGCTGG CCAAGCAGAC CGCCCAGCGG CGCATTGTGG GCGGCGATGA TGCGGGCTTC 
GGCTCCTTTC CCTGGCAGGC CTACATCCGC ATCGGTTCCT CCAGGTGCGG AGGTTCGTTG 
ATCTCGCGAC GACACGTGGT CACCGCTGGT CATTGTGTGG CCCGGGCCAC TCCGCGACAG 
GTGCATGTCA CGCTGGGTGA TTACGTGATC AACTCGGCCG TGGAGCCACT GCCGGCCTAC 
ACCTTCGGAG TCCGCCGGAT CGACGTGCAT CCGTACTTCA AGTTCACGCC GCAGGCGGAC 
CGCTTCGACA TCTCCGTGCT GACGCTAGAG CGTACCGTGC ACTTCATGCC CCACATAGCT 
CCGATTTGCC TGCCCGAGAA GAACGAGGAC TTCCTGGGCA AATTTGGCTG GGCAGCCGGC 
TGGGGAGCTC TGAACCCGGG CTCCCGGCTG CGTCCCAAGA CGCTGCAGGC CGTGGATGTG 
CCCGTGATCG AGAACCGAAT CTGCGAGCGC TGGCACCGGC AGAACGGCAT CAACGTGGTC 
ATCTACCAGG AGATGCTCTG CGCGGGCTAT CGCAACGGTG GCAAGGACTC GTGCCAAGGC 
GACTCTGGAG GACCTTTGAT GCACGACAAG AATGGGCGCT GGTACCTGAT TGGTGTGGTC 
TCTGCTGGTT ACTCCTGCGC GTCCCGCGGT CAGCCGGGAA TCTATCACAG CGTCAGCAAG 
ACCGTGGACT GGGTATCCTA CGTCGTCGGA CTCACGATGA ACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8170 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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