Np cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Np
Entrez Gene ID	35904
Full Name	Notopleural
Synonyms	CG11824,CG34350,CG8181,Dmel\CG34350,Dmel_CG11824,Dmel_CG8181,SP59,np
General protein information	Preferred Names Notopleural Names CG34350 gene product from transcript CG34350-RB CG34350-PA CG34350-PB Np-PA Np-PB notopleural
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45A1-45A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_136593.3	NP_610437.2	notopleural, isoform A
NM_001259284.2	NP_001246213.1	notopleural, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Np gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Np cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09303	NM_001259284.2 Latest version!	Drosophila melanogaster notopleural, transcript variant B (Np), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa09303	NM_136593.3 Latest version!	Drosophila melanogaster notopleural, transcript variant A (Np), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Np ( NM_136593.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610437.2 Drosophila melanogaster notopleural, transcript variant A (Np), mRNA.

Price & Availability↑

CloneID	OFa09303
Clone ID Related Accession (Same CDS sequence)	NM_136593.3 , NM_001259284.2
Accession Version	NM_136593.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	notopleural, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136593.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTGCTC AACACACGGA CACAGGCACC TATACGTACA CAGCAGCCAG TAGGGCGGTA 
GAAGCCTCAG ATCTGACACC CAAAAGAATT GGAGCACCAG CGATAGCCAC TTCTAGAATG 
CCACTCACCA GCCTCGTCTT CAGCCTCCTC ACGCTGGGCA GCCTGATGCT GTTGACGGCA 
GACGCCAGTC CCGTCTTCGT GGATAATCCC AGCTTAGCAC AGTTCCAATC TGGTCGTAAT 
ATCCGTCATC TGCCCTGCAT CGTTCGCAAA TCGGGACGCT CTGGCATCTG TATGTTCGCC 
ATCGATTGCA TCAAGCAGAA TGGTACCCAC CTGGGCACCT GCATCGATCG CTTTTACTTT 
GGCTCGTGCT GCGCCATGAA GGAGGAAGCC TCGCTCTTTG CGCCAGAGAT CAACGACAAC 
AGCATTGACC AGAACACCAT CTCCCACTTT TCCCACGAGT CCACCACGCT GCCAACCAGT 
GCCCTCTTCA AGCTAACCAC AGAAAGCAGC TTGTCCAGTA GTAGCAGCAG CGGTAGCAGC 
ACCGCCCATC ACCACACCAC CAACGAGCTG CTCAAGAACG TCACCCAGTC CTATCTGAGC 
ACCGCGAAAC CGGCACCTGT TCGCACCACC AGCAACGGCA CGCTCTCAAC CACACGCCGC 
CCACCGCTGG CACACACCAC CCACAAGAAC TCGCACTACG TGGTGCGCAC CACTATGAAA 
CCCAGTGCCG CACCGACAAC AACGATTACG CCAACTGTTA AGCCACCGAG GAGGCGCACA 
ACCACCGCCA AGCCGGTGCT AACAACTGCT CCGATTGTGG AGCAACGCAT CGAAACCACG 
TCGGTACCCA CCAAGTTTGT GACTTTCCAA ATTGTGGAGA CGACGACGCC AGCGGTGACC 
GAACCCACTC ACCAGCAGGA GACAACAACC ACTACGACCA GGCGACACAC AAGGCCAACG 
GGAAGCAGCT CCGTAGCCAG TAGGACAACC AGCAAAACAA CAACCACTAC CACCACCACA 
ACAACAACCA CAGCAGCTCC ACCCACAACA ACGAGCACGA GAGCGGCCAC AACCACCCAA 
CGTCCAACAC CACCGCTGCG CACCACCACG AGCAAGCCGC ACAAAACCCA TTCCAGCAGG 
AGGCCAGCAC CTGCGAAAAA GCCAGTGAGT ACGACAACAA CCACCGCCAG TGGAACGCAA 
TCCACACGGA AGCCGGCTGA TAGTCTGGCC AGCGGCAGCA CCACTGCCAA TGGCACCACA 
GCCACCACAT TGCCAGCTCG AAGGCCCGTG ACTCAGACGA GCAGCAGCAG TACCACCACC 
ACCAGCACAA CATCGACAAT AACAACCACC GTGTTTAAGG ACGAACTGCC CAAGAACCGC 
ACCACAACGA GGCTGCCAGA ACGCACCACT CAAGCTCCGC GACCCAGGCC GAAACCCACC 
GGGGAAATCG TCAAGGTGCC CGCTCTGCTT GCTGAGCCAA CAACAACCAC CGTGACAAAG 
GGTTCATCCA GCAGCAGCAG CAGTTCTCCA AAGCCACCAG CAAGTAGCAC CACTCCTTTG 
GTAACCAAAA AGAAGCCAAC CACCACCAAC GCGCCAACTA CCACTACCAC TACTACAACA 
ACAACAACAA CAACCACAAC ACCTAAACCC ACTCGAAGAA CAAAGCCTCC CACAACAACA 
ACCACTACTA CTACCAAGGC TACAACAACT ACCACTAAGG CGACAACAAC AACAACTACT 
GCAAAACCAC TGATCACCAC GGAACCACCC ACAAGTGCAC CCCTAACAAC AACCACCAAA 
AAGACCGGCC TGATAACTTG GACCGATGTG GAGGCTGAGC CACCGACTAA TGCCAGTATT 
TCGAACGATT GGCAGCCTGC ACCACCTTCT AATTGGTTGC CACTGACCAC TGAATCAAGT 
GAAACAGCTG ACAAAACATC AGCGTCCACA GCACCACCCA GCGCCACCGA CAAAGTGGTC 
ATCTCGGTGG CCACCAGTGT CAGCGAAGTG GAGACCCACG GCCCGGGCAA GCCGCAGAAG 
ACCACAAAGC CACCGAAACC CAGCAGCACA GCCAAGCCCA CAACAACAAC CACCGCAGCA 
GCGACAACCA TAGTGAAAAC CACAGAGGCA CCAGCGAGCA GTATAGAATT AGCCTCATCT 
ACATCATCTT ACACGGATCT GGGCAGTGAT TTGGCAGCCA GCAGCAGCAG CAGCACCACC 
ACCACCACCA GCAGTCCCAC AACCGCAGCT GAAACCACCG ATTACAGCGG CGAAGAACCG 
AACACAACGA CCATCGAGGC TACAGGCAGC ACCACCGTGG CACCCGCCAA TGCATTGGAG 
GGCGTCGATT ACAAAGAAGT TTGCGGTCGT CGCATGTTCC CCGAGCCCCG AATCGTTGGT 
GGAGCTAATG CGGCCTTTGG ACGCTGGCCC TGGCAGATCT CGCTGCGCCA GTGGCGCACC 
TCCACCTACC TGCACAAGTG CGGAGCTGCC CTGCTGAACG AGAACTGGGC GATCACGGCG 
GCCCATTGCG TGGATAATGT GCCACCTTCC GATCTCCTGC TGCGCCTGGG AGAGTATGAT 
CTGGCGGAGG AGGAGGAGCC CTACGGCTAC CAGGAGCGAC GAGTGCAGAT CGTCGCCTCG 
CATCCGCAGT TCGATCCGCG CACCTTCGAG TACGATCTGG CCCTGCTCAG GTTCTACGAG 
CCCGTCATCT TCCAGCCGAA CATCATTCCC GTTTGCGTTC CCGACAACGA TGAGAACTTC 
ATTGGCCAGA CCGCCTTCGT CACCGGCTGG GGCCGGCTCT ATGAGGATGG ACCCCTGCCC 
AGTGTGCTGC AGGAGGTTGC CGTCCCCGTG ATCAACAACA CCATATGCGA GTCCATGTAC 
CGATCCGCGG GCTACATCGA GCACATACCG CACATATTCA TCTGCGCCGG CTGGAAGAAG 
GGCGGGTACG ACTCCTGCGA AGGCGATTCC GGTGGCCCCA TGGTGCTGCA GCGTGAGTCC 
GACAAGCGTT TCCACCTCGG CGGTGTCATA TCGTGGGGCA TTGGATGTGC GGAGGCCAAC 
CAGCCTGGCG TTTATACCCG CATCTCGGAA TTCCGGGACT GGATCAACCA GATCCTGCAG 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610437.2
CDS	374..3499
Translation	MGAQHTDTGTYTYTAASRAVEASDLTPKRIGAPAIATSRMPLTSLVFSLLTLGSLMLLTADASPVFVDNPSLAQFQSGRNIRHLPCIVRKSGRSGICMFAIDCIKQNGTHLGTCIDRFYFGSCCAMKEEASLFAPEINDNSIDQNTISHFSHESTTLPTSALFKLTTESSLSSSSSSGSSTAHHHTTNELLKNVTQSYLSTAKPAPVRTTSNGTLSTTRRPPLAHTTHKNSHYVVRTTMKPSAAPTTTITPTVKPPRRRTTTAKPVLTTAPIVEQRIETTSVPTKFVTFQIVETTTPAVTEPTHQQETTTTTTRRHTRPTGSSSVASRTTSKTTTTTTTTTTTTAAPPTTTSTRAATTTQRPTPPLRTTTSKPHKTHSSRRPAPAKKPVSTTTTTASGTQSTRKPADSLASGSTTANGTTATTLPARRPVTQTSSSSTTTTSTTSTITTTVFKDELPKNRTTTRLPERTTQAPRPRPKPTGEIVKVPALLAEPTTTTVTKGSSSSSSSSPKPPASSTTPLVTKKKPTTTNAPTTTTTTTTTTTTTTTPKPTRRTKPPTTTTTTTTKATTTTTKATTTTTTAKPLITTEPPTSAPLTTTTKKTGLITWTDVEAEPPTNASISNDWQPAPPSNWLPLTTESSETADKTSASTAPPSATDKVVISVATSVSEVETHGPGKPQKTTKPPKPSSTAKPTTTTTAAATTIVKTTEAPASSIELASSTSSYTDLGSDLAASSSSSTTTTTSSPTTAAETTDYSGEEPNTTTIEATGSTTVAPANALEGVDYKEVCGRRMFPEPRIVGGANAAFGRWPWQISLRQWRTSTYLHKCGAALLNENWAITAAHCVDNVPPSDLLLRLGEYDLAEEEEPYGYQERRVQIVASHPQFDPRTFEYDLALLRFYEPVIFQPNIIPVCVPDNDENFIGQTAFVTGWGRLYEDGPLPSVLQEVAVPVINNTICESMYRSAGYIEHIPHIFICAGWKKGGYDSCEGDSGGPMVLQRESDKRFHLGGVISWGIGCAEANQPGVYTRISEFRDWINQILQF

Target ORF information:

RefSeq Version	NM_136593.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster notopleural, transcript variant A (Np), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136593.3

ORF Insert Sequence:

ATGGGTGCTC AACACACGGA CACAGGCACC TATACGTACA CAGCAGCCAG TAGGGCGGTA 
GAAGCCTCAG ATCTGACACC CAAAAGAATT GGAGCACCAG CGATAGCCAC TTCTAGAATG 
CCACTCACCA GCCTCGTCTT CAGCCTCCTC ACGCTGGGCA GCCTGATGCT GTTGACGGCA 
GACGCCAGTC CCGTCTTCGT GGATAATCCC AGCTTAGCAC AGTTCCAATC TGGTCGTAAT 
ATCCGTCATC TGCCCTGCAT CGTTCGCAAA TCGGGACGCT CTGGCATCTG TATGTTCGCC 
ATCGATTGCA TCAAGCAGAA TGGTACCCAC CTGGGCACCT GCATCGATCG CTTTTACTTT 
GGCTCGTGCT GCGCCATGAA GGAGGAAGCC TCGCTCTTTG CGCCAGAGAT CAACGACAAC 
AGCATTGACC AGAACACCAT CTCCCACTTT TCCCACGAGT CCACCACGCT GCCAACCAGT 
GCCCTCTTCA AGCTAACCAC AGAAAGCAGC TTGTCCAGTA GTAGCAGCAG CGGTAGCAGC 
ACCGCCCATC ACCACACCAC CAACGAGCTG CTCAAGAACG TCACCCAGTC CTATCTGAGC 
ACCGCGAAAC CGGCACCTGT TCGCACCACC AGCAACGGCA CGCTCTCAAC CACACGCCGC 
CCACCGCTGG CACACACCAC CCACAAGAAC TCGCACTACG TGGTGCGCAC CACTATGAAA 
CCCAGTGCCG CACCGACAAC AACGATTACG CCAACTGTTA AGCCACCGAG GAGGCGCACA 
ACCACCGCCA AGCCGGTGCT AACAACTGCT CCGATTGTGG AGCAACGCAT CGAAACCACG 
TCGGTACCCA CCAAGTTTGT GACTTTCCAA ATTGTGGAGA CGACGACGCC AGCGGTGACC 
GAACCCACTC ACCAGCAGGA GACAACAACC ACTACGACCA GGCGACACAC AAGGCCAACG 
GGAAGCAGCT CCGTAGCCAG TAGGACAACC AGCAAAACAA CAACCACTAC CACCACCACA 
ACAACAACCA CAGCAGCTCC ACCCACAACA ACGAGCACGA GAGCGGCCAC AACCACCCAA 
CGTCCAACAC CACCGCTGCG CACCACCACG AGCAAGCCGC ACAAAACCCA TTCCAGCAGG 
AGGCCAGCAC CTGCGAAAAA GCCAGTGAGT ACGACAACAA CCACCGCCAG TGGAACGCAA 
TCCACACGGA AGCCGGCTGA TAGTCTGGCC AGCGGCAGCA CCACTGCCAA TGGCACCACA 
GCCACCACAT TGCCAGCTCG AAGGCCCGTG ACTCAGACGA GCAGCAGCAG TACCACCACC 
ACCAGCACAA CATCGACAAT AACAACCACC GTGTTTAAGG ACGAACTGCC CAAGAACCGC 
ACCACAACGA GGCTGCCAGA ACGCACCACT CAAGCTCCGC GACCCAGGCC GAAACCCACC 
GGGGAAATCG TCAAGGTGCC CGCTCTGCTT GCTGAGCCAA CAACAACCAC CGTGACAAAG 
GGTTCATCCA GCAGCAGCAG CAGTTCTCCA AAGCCACCAG CAAGTAGCAC CACTCCTTTG 
GTAACCAAAA AGAAGCCAAC CACCACCAAC GCGCCAACTA CCACTACCAC TACTACAACA 
ACAACAACAA CAACCACAAC ACCTAAACCC ACTCGAAGAA CAAAGCCTCC CACAACAACA 
ACCACTACTA CTACCAAGGC TACAACAACT ACCACTAAGG CGACAACAAC AACAACTACT 
GCAAAACCAC TGATCACCAC GGAACCACCC ACAAGTGCAC CCCTAACAAC AACCACCAAA 
AAGACCGGCC TGATAACTTG GACCGATGTG GAGGCTGAGC CACCGACTAA TGCCAGTATT 
TCGAACGATT GGCAGCCTGC ACCACCTTCT AATTGGTTGC CACTGACCAC TGAATCAAGT 
GAAACAGCTG ACAAAACATC AGCGTCCACA GCACCACCCA GCGCCACCGA CAAAGTGGTC 
ATCTCGGTGG CCACCAGTGT CAGCGAAGTG GAGACCCACG GCCCGGGCAA GCCGCAGAAG 
ACCACAAAGC CACCGAAACC CAGCAGCACA GCCAAGCCCA CAACAACAAC CACCGCAGCA 
GCGACAACCA TAGTGAAAAC CACAGAGGCA CCAGCGAGCA GTATAGAATT AGCCTCATCT 
ACATCATCTT ACACGGATCT GGGCAGTGAT TTGGCAGCCA GCAGCAGCAG CAGCACCACC 
ACCACCACCA GCAGTCCCAC AACCGCAGCT GAAACCACCG ATTACAGCGG CGAAGAACCG 
AACACAACGA CCATCGAGGC TACAGGCAGC ACCACCGTGG CACCCGCCAA TGCATTGGAG 
GGCGTCGATT ACAAAGAAGT TTGCGGTCGT CGCATGTTCC CCGAGCCCCG AATCGTTGGT 
GGAGCTAATG CGGCCTTTGG ACGCTGGCCC TGGCAGATCT CGCTGCGCCA GTGGCGCACC 
TCCACCTACC TGCACAAGTG CGGAGCTGCC CTGCTGAACG AGAACTGGGC GATCACGGCG 
GCCCATTGCG TGGATAATGT GCCACCTTCC GATCTCCTGC TGCGCCTGGG AGAGTATGAT 
CTGGCGGAGG AGGAGGAGCC CTACGGCTAC CAGGAGCGAC GAGTGCAGAT CGTCGCCTCG 
CATCCGCAGT TCGATCCGCG CACCTTCGAG TACGATCTGG CCCTGCTCAG GTTCTACGAG 
CCCGTCATCT TCCAGCCGAA CATCATTCCC GTTTGCGTTC CCGACAACGA TGAGAACTTC 
ATTGGCCAGA CCGCCTTCGT CACCGGCTGG GGCCGGCTCT ATGAGGATGG ACCCCTGCCC 
AGTGTGCTGC AGGAGGTTGC CGTCCCCGTG ATCAACAACA CCATATGCGA GTCCATGTAC 
CGATCCGCGG GCTACATCGA GCACATACCG CACATATTCA TCTGCGCCGG CTGGAAGAAG 
GGCGGGTACG ACTCCTGCGA AGGCGATTCC GGTGGCCCCA TGGTGCTGCA GCGTGAGTCC 
GACAAGCGTT TCCACCTCGG CGGTGTCATA TCGTGGGGCA TTGGATGTGC GGAGGCCAAC 
CAGCCTGGCG TTTATACCCG CATCTCGGAA TTCCGGGACT GGATCAACCA GATCCTGCAG 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Np ( NM_001259284.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246213.1 Drosophila melanogaster notopleural, transcript variant B (Np), mRNA.

Price & Availability↑

CloneID	OFa09303
Clone ID Related Accession (Same CDS sequence)	NM_136593.3 , NM_001259284.2
Accession Version	NM_001259284.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	notopleural, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259284.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTGCTC AACACACGGA CACAGGCACC TATACGTACA CAGCAGCCAG TAGGGCGGTA 
GAAGCCTCAG ATCTGACACC CAAAAGAATT GGAGCACCAG CGATAGCCAC TTCTAGAATG 
CCACTCACCA GCCTCGTCTT CAGCCTCCTC ACGCTGGGCA GCCTGATGCT GTTGACGGCA 
GACGCCAGTC CCGTCTTCGT GGATAATCCC AGCTTAGCAC AGTTCCAATC TGGTCGTAAT 
ATCCGTCATC TGCCCTGCAT CGTTCGCAAA TCGGGACGCT CTGGCATCTG TATGTTCGCC 
ATCGATTGCA TCAAGCAGAA TGGTACCCAC CTGGGCACCT GCATCGATCG CTTTTACTTT 
GGCTCGTGCT GCGCCATGAA GGAGGAAGCC TCGCTCTTTG CGCCAGAGAT CAACGACAAC 
AGCATTGACC AGAACACCAT CTCCCACTTT TCCCACGAGT CCACCACGCT GCCAACCAGT 
GCCCTCTTCA AGCTAACCAC AGAAAGCAGC TTGTCCAGTA GTAGCAGCAG CGGTAGCAGC 
ACCGCCCATC ACCACACCAC CAACGAGCTG CTCAAGAACG TCACCCAGTC CTATCTGAGC 
ACCGCGAAAC CGGCACCTGT TCGCACCACC AGCAACGGCA CGCTCTCAAC CACACGCCGC 
CCACCGCTGG CACACACCAC CCACAAGAAC TCGCACTACG TGGTGCGCAC CACTATGAAA 
CCCAGTGCCG CACCGACAAC AACGATTACG CCAACTGTTA AGCCACCGAG GAGGCGCACA 
ACCACCGCCA AGCCGGTGCT AACAACTGCT CCGATTGTGG AGCAACGCAT CGAAACCACG 
TCGGTACCCA CCAAGTTTGT GACTTTCCAA ATTGTGGAGA CGACGACGCC AGCGGTGACC 
GAACCCACTC ACCAGCAGGA GACAACAACC ACTACGACCA GGCGACACAC AAGGCCAACG 
GGAAGCAGCT CCGTAGCCAG TAGGACAACC AGCAAAACAA CAACCACTAC CACCACCACA 
ACAACAACCA CAGCAGCTCC ACCCACAACA ACGAGCACGA GAGCGGCCAC AACCACCCAA 
CGTCCAACAC CACCGCTGCG CACCACCACG AGCAAGCCGC ACAAAACCCA TTCCAGCAGG 
AGGCCAGCAC CTGCGAAAAA GCCAGTGAGT ACGACAACAA CCACCGCCAG TGGAACGCAA 
TCCACACGGA AGCCGGCTGA TAGTCTGGCC AGCGGCAGCA CCACTGCCAA TGGCACCACA 
GCCACCACAT TGCCAGCTCG AAGGCCCGTG ACTCAGACGA GCAGCAGCAG TACCACCACC 
ACCAGCACAA CATCGACAAT AACAACCACC GTGTTTAAGG ACGAACTGCC CAAGAACCGC 
ACCACAACGA GGCTGCCAGA ACGCACCACT CAAGCTCCGC GACCCAGGCC GAAACCCACC 
GGGGAAATCG TCAAGGTGCC CGCTCTGCTT GCTGAGCCAA CAACAACCAC CGTGACAAAG 
GGTTCATCCA GCAGCAGCAG CAGTTCTCCA AAGCCACCAG CAAGTAGCAC CACTCCTTTG 
GTAACCAAAA AGAAGCCAAC CACCACCAAC GCGCCAACTA CCACTACCAC TACTACAACA 
ACAACAACAA CAACCACAAC ACCTAAACCC ACTCGAAGAA CAAAGCCTCC CACAACAACA 
ACCACTACTA CTACCAAGGC TACAACAACT ACCACTAAGG CGACAACAAC AACAACTACT 
GCAAAACCAC TGATCACCAC GGAACCACCC ACAAGTGCAC CCCTAACAAC AACCACCAAA 
AAGACCGGCC TGATAACTTG GACCGATGTG GAGGCTGAGC CACCGACTAA TGCCAGTATT 
TCGAACGATT GGCAGCCTGC ACCACCTTCT AATTGGTTGC CACTGACCAC TGAATCAAGT 
GAAACAGCTG ACAAAACATC AGCGTCCACA GCACCACCCA GCGCCACCGA CAAAGTGGTC 
ATCTCGGTGG CCACCAGTGT CAGCGAAGTG GAGACCCACG GCCCGGGCAA GCCGCAGAAG 
ACCACAAAGC CACCGAAACC CAGCAGCACA GCCAAGCCCA CAACAACAAC CACCGCAGCA 
GCGACAACCA TAGTGAAAAC CACAGAGGCA CCAGCGAGCA GTATAGAATT AGCCTCATCT 
ACATCATCTT ACACGGATCT GGGCAGTGAT TTGGCAGCCA GCAGCAGCAG CAGCACCACC 
ACCACCACCA GCAGTCCCAC AACCGCAGCT GAAACCACCG ATTACAGCGG CGAAGAACCG 
AACACAACGA CCATCGAGGC TACAGGCAGC ACCACCGTGG CACCCGCCAA TGCATTGGAG 
GGCGTCGATT ACAAAGAAGT TTGCGGTCGT CGCATGTTCC CCGAGCCCCG AATCGTTGGT 
GGAGCTAATG CGGCCTTTGG ACGCTGGCCC TGGCAGATCT CGCTGCGCCA GTGGCGCACC 
TCCACCTACC TGCACAAGTG CGGAGCTGCC CTGCTGAACG AGAACTGGGC GATCACGGCG 
GCCCATTGCG TGGATAATGT GCCACCTTCC GATCTCCTGC TGCGCCTGGG AGAGTATGAT 
CTGGCGGAGG AGGAGGAGCC CTACGGCTAC CAGGAGCGAC GAGTGCAGAT CGTCGCCTCG 
CATCCGCAGT TCGATCCGCG CACCTTCGAG TACGATCTGG CCCTGCTCAG GTTCTACGAG 
CCCGTCATCT TCCAGCCGAA CATCATTCCC GTTTGCGTTC CCGACAACGA TGAGAACTTC 
ATTGGCCAGA CCGCCTTCGT CACCGGCTGG GGCCGGCTCT ATGAGGATGG ACCCCTGCCC 
AGTGTGCTGC AGGAGGTTGC CGTCCCCGTG ATCAACAACA CCATATGCGA GTCCATGTAC 
CGATCCGCGG GCTACATCGA GCACATACCG CACATATTCA TCTGCGCCGG CTGGAAGAAG 
GGCGGGTACG ACTCCTGCGA AGGCGATTCC GGTGGCCCCA TGGTGCTGCA GCGTGAGTCC 
GACAAGCGTT TCCACCTCGG CGGTGTCATA TCGTGGGGCA TTGGATGTGC GGAGGCCAAC 
CAGCCTGGCG TTTATACCCG CATCTCGGAA TTCCGGGACT GGATCAACCA GATCCTGCAG 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246213.1
CDS	100..3225
Translation	MGAQHTDTGTYTYTAASRAVEASDLTPKRIGAPAIATSRMPLTSLVFSLLTLGSLMLLTADASPVFVDNPSLAQFQSGRNIRHLPCIVRKSGRSGICMFAIDCIKQNGTHLGTCIDRFYFGSCCAMKEEASLFAPEINDNSIDQNTISHFSHESTTLPTSALFKLTTESSLSSSSSSGSSTAHHHTTNELLKNVTQSYLSTAKPAPVRTTSNGTLSTTRRPPLAHTTHKNSHYVVRTTMKPSAAPTTTITPTVKPPRRRTTTAKPVLTTAPIVEQRIETTSVPTKFVTFQIVETTTPAVTEPTHQQETTTTTTRRHTRPTGSSSVASRTTSKTTTTTTTTTTTTAAPPTTTSTRAATTTQRPTPPLRTTTSKPHKTHSSRRPAPAKKPVSTTTTTASGTQSTRKPADSLASGSTTANGTTATTLPARRPVTQTSSSSTTTTSTTSTITTTVFKDELPKNRTTTRLPERTTQAPRPRPKPTGEIVKVPALLAEPTTTTVTKGSSSSSSSSPKPPASSTTPLVTKKKPTTTNAPTTTTTTTTTTTTTTTPKPTRRTKPPTTTTTTTTKATTTTTKATTTTTTAKPLITTEPPTSAPLTTTTKKTGLITWTDVEAEPPTNASISNDWQPAPPSNWLPLTTESSETADKTSASTAPPSATDKVVISVATSVSEVETHGPGKPQKTTKPPKPSSTAKPTTTTTAAATTIVKTTEAPASSIELASSTSSYTDLGSDLAASSSSSTTTTTSSPTTAAETTDYSGEEPNTTTIEATGSTTVAPANALEGVDYKEVCGRRMFPEPRIVGGANAAFGRWPWQISLRQWRTSTYLHKCGAALLNENWAITAAHCVDNVPPSDLLLRLGEYDLAEEEEPYGYQERRVQIVASHPQFDPRTFEYDLALLRFYEPVIFQPNIIPVCVPDNDENFIGQTAFVTGWGRLYEDGPLPSVLQEVAVPVINNTICESMYRSAGYIEHIPHIFICAGWKKGGYDSCEGDSGGPMVLQRESDKRFHLGGVISWGIGCAEANQPGVYTRISEFRDWINQILQF

Target ORF information:

RefSeq Version	NM_001259284.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster notopleural, transcript variant B (Np), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259284.2

ORF Insert Sequence:

ATGGGTGCTC AACACACGGA CACAGGCACC TATACGTACA CAGCAGCCAG TAGGGCGGTA 
GAAGCCTCAG ATCTGACACC CAAAAGAATT GGAGCACCAG CGATAGCCAC TTCTAGAATG 
CCACTCACCA GCCTCGTCTT CAGCCTCCTC ACGCTGGGCA GCCTGATGCT GTTGACGGCA 
GACGCCAGTC CCGTCTTCGT GGATAATCCC AGCTTAGCAC AGTTCCAATC TGGTCGTAAT 
ATCCGTCATC TGCCCTGCAT CGTTCGCAAA TCGGGACGCT CTGGCATCTG TATGTTCGCC 
ATCGATTGCA TCAAGCAGAA TGGTACCCAC CTGGGCACCT GCATCGATCG CTTTTACTTT 
GGCTCGTGCT GCGCCATGAA GGAGGAAGCC TCGCTCTTTG CGCCAGAGAT CAACGACAAC 
AGCATTGACC AGAACACCAT CTCCCACTTT TCCCACGAGT CCACCACGCT GCCAACCAGT 
GCCCTCTTCA AGCTAACCAC AGAAAGCAGC TTGTCCAGTA GTAGCAGCAG CGGTAGCAGC 
ACCGCCCATC ACCACACCAC CAACGAGCTG CTCAAGAACG TCACCCAGTC CTATCTGAGC 
ACCGCGAAAC CGGCACCTGT TCGCACCACC AGCAACGGCA CGCTCTCAAC CACACGCCGC 
CCACCGCTGG CACACACCAC CCACAAGAAC TCGCACTACG TGGTGCGCAC CACTATGAAA 
CCCAGTGCCG CACCGACAAC AACGATTACG CCAACTGTTA AGCCACCGAG GAGGCGCACA 
ACCACCGCCA AGCCGGTGCT AACAACTGCT CCGATTGTGG AGCAACGCAT CGAAACCACG 
TCGGTACCCA CCAAGTTTGT GACTTTCCAA ATTGTGGAGA CGACGACGCC AGCGGTGACC 
GAACCCACTC ACCAGCAGGA GACAACAACC ACTACGACCA GGCGACACAC AAGGCCAACG 
GGAAGCAGCT CCGTAGCCAG TAGGACAACC AGCAAAACAA CAACCACTAC CACCACCACA 
ACAACAACCA CAGCAGCTCC ACCCACAACA ACGAGCACGA GAGCGGCCAC AACCACCCAA 
CGTCCAACAC CACCGCTGCG CACCACCACG AGCAAGCCGC ACAAAACCCA TTCCAGCAGG 
AGGCCAGCAC CTGCGAAAAA GCCAGTGAGT ACGACAACAA CCACCGCCAG TGGAACGCAA 
TCCACACGGA AGCCGGCTGA TAGTCTGGCC AGCGGCAGCA CCACTGCCAA TGGCACCACA 
GCCACCACAT TGCCAGCTCG AAGGCCCGTG ACTCAGACGA GCAGCAGCAG TACCACCACC 
ACCAGCACAA CATCGACAAT AACAACCACC GTGTTTAAGG ACGAACTGCC CAAGAACCGC 
ACCACAACGA GGCTGCCAGA ACGCACCACT CAAGCTCCGC GACCCAGGCC GAAACCCACC 
GGGGAAATCG TCAAGGTGCC CGCTCTGCTT GCTGAGCCAA CAACAACCAC CGTGACAAAG 
GGTTCATCCA GCAGCAGCAG CAGTTCTCCA AAGCCACCAG CAAGTAGCAC CACTCCTTTG 
GTAACCAAAA AGAAGCCAAC CACCACCAAC GCGCCAACTA CCACTACCAC TACTACAACA 
ACAACAACAA CAACCACAAC ACCTAAACCC ACTCGAAGAA CAAAGCCTCC CACAACAACA 
ACCACTACTA CTACCAAGGC TACAACAACT ACCACTAAGG CGACAACAAC AACAACTACT 
GCAAAACCAC TGATCACCAC GGAACCACCC ACAAGTGCAC CCCTAACAAC AACCACCAAA 
AAGACCGGCC TGATAACTTG GACCGATGTG GAGGCTGAGC CACCGACTAA TGCCAGTATT 
TCGAACGATT GGCAGCCTGC ACCACCTTCT AATTGGTTGC CACTGACCAC TGAATCAAGT 
GAAACAGCTG ACAAAACATC AGCGTCCACA GCACCACCCA GCGCCACCGA CAAAGTGGTC 
ATCTCGGTGG CCACCAGTGT CAGCGAAGTG GAGACCCACG GCCCGGGCAA GCCGCAGAAG 
ACCACAAAGC CACCGAAACC CAGCAGCACA GCCAAGCCCA CAACAACAAC CACCGCAGCA 
GCGACAACCA TAGTGAAAAC CACAGAGGCA CCAGCGAGCA GTATAGAATT AGCCTCATCT 
ACATCATCTT ACACGGATCT GGGCAGTGAT TTGGCAGCCA GCAGCAGCAG CAGCACCACC 
ACCACCACCA GCAGTCCCAC AACCGCAGCT GAAACCACCG ATTACAGCGG CGAAGAACCG 
AACACAACGA CCATCGAGGC TACAGGCAGC ACCACCGTGG CACCCGCCAA TGCATTGGAG 
GGCGTCGATT ACAAAGAAGT TTGCGGTCGT CGCATGTTCC CCGAGCCCCG AATCGTTGGT 
GGAGCTAATG CGGCCTTTGG ACGCTGGCCC TGGCAGATCT CGCTGCGCCA GTGGCGCACC 
TCCACCTACC TGCACAAGTG CGGAGCTGCC CTGCTGAACG AGAACTGGGC GATCACGGCG 
GCCCATTGCG TGGATAATGT GCCACCTTCC GATCTCCTGC TGCGCCTGGG AGAGTATGAT 
CTGGCGGAGG AGGAGGAGCC CTACGGCTAC CAGGAGCGAC GAGTGCAGAT CGTCGCCTCG 
CATCCGCAGT TCGATCCGCG CACCTTCGAG TACGATCTGG CCCTGCTCAG GTTCTACGAG 
CCCGTCATCT TCCAGCCGAA CATCATTCCC GTTTGCGTTC CCGACAACGA TGAGAACTTC 
ATTGGCCAGA CCGCCTTCGT CACCGGCTGG GGCCGGCTCT ATGAGGATGG ACCCCTGCCC 
AGTGTGCTGC AGGAGGTTGC CGTCCCCGTG ATCAACAACA CCATATGCGA GTCCATGTAC 
CGATCCGCGG GCTACATCGA GCACATACCG CACATATTCA TCTGCGCCGG CTGGAAGAAG 
GGCGGGTACG ACTCCTGCGA AGGCGATTCC GGTGGCCCCA TGGTGCTGCA GCGTGAGTCC 
GACAAGCGTT TCCACCTCGG CGGTGTCATA TCGTGGGGCA TTGGATGTGC GGAGGCCAAC 
CAGCCTGGCG TTTATACCCG CATCTCGGAA TTCCGGGACT GGATCAACCA GATCCTGCAG 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Np cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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