ptc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ptc
Entrez Gene ID	35851
Full Name	patched
Synonyms	BcDNA:RH36596,CG2411,Conf,Dmel\CG2411,PTC,Ptc,Ptc_Dm,Ptch,com4,dPtc,l(2)k02507,pat,ptch,rubr,tuf
General protein information	Preferred Names patched Names CG2411 gene product from transcript CG2411-RA CG2411-PA compaction defects 4 confused patch ptc-PA rubberneck tufted
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44D5-44E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_078937.4	NP_523661.2	patched

Pathways from BioSystems

Homologies

	PTCH1	XP_001111868.2
Canis lupus familiaris (dog)	PTCH1	XP_003638919.1
Rattus norvegicus (Norway rat)	Ptch1	NP_446018.1
Gallus gallus (chicken)	PTCH1	NP_990291.1
Caenorhabditis elegans (roundworm)	ptc-1	NP_495662.2
Danio rerio (zebrafish)	ptch1	XP_001922161.4
Caenorhabditis elegans (roundworm)	ptc-3	NP_494384.2
Xenopus tropicalis (tropical clawed frog)	ptch1	XP_002935019.2
Pan troglodytes (chimpanzee)	PTCH1	XP_003312241.1
Bos taurus (cattle)	PTCH1	NP_001192808.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011395	XP_317925.4
Caenorhabditis elegans (roundworm)	ptc-2	NP_495218.2
Mus musculus (house mouse)	Ptch1	NP_032983.1
Homo sapiens (human)	PTCH1	NP_000255.2
Drosophila melanogaster (fruit fly)	ptc	NP_523661.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a mutation of the PTC PY motif, which mediates binding of C2-WW-HECT family members, inhibits its internalization but not its autonomous and non-autonomous signalling activities. the two related UB-C2-WW-HECT ligases NEDD4 and SU(DX) regulate PTC trafficking and finely tune its accumulation through partially redundant but distinct functions
Title: Control of the dynamics and homeostasis of the Drosophila Hedgehog receptor Patched by two C2-WW-HECT-E3 Ubiquitin ligases.

in vivo study demonstrates that the PI3K-cIII complex is essential for downregulating Patched via the endosomal-lysosomal pathway to execute axon pruning
Title: The PI3K class III complex promotes axon pruning by downregulating a Ptc-derived signal via endosome-lysosomal degradation.

Ihog and Boi elicit Hh signaling via Ptc but do not aid Ptc in sequestering the Hh ligand
Title: Ihog and Boi elicit Hh signaling via Ptc but do not aid Ptc in sequestering the Hh ligand.

Kto-Skd affects the A/P-axial development of the whole wing disc. Kto can interact with Cubitus interruptus (Ci), bind to the Ci-binding region on ptc promoter, which are both regulated by Hh signals to down-regulate ptc expression.
Title: The Kto-Skd complex can regulate ptc expression by interacting with Cubitus interruptus (Ci) in the Hedgehog signaling pathway.

Self-induced patched receptor down-regulation modulates cell sensitivity to the hedgehog morphogen gradient.
Title: Self-induced patched receptor down-regulation modulates cell sensitivity to the hedgehog morphogen gradient.

The following ptc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12790	NM_078937.4 Latest version!	Drosophila melanogaster patched (ptc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ptc ( NM_078937.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523661.2 Drosophila melanogaster patched (ptc), mRNA.

Price & Availability↑

CloneID	OFa12790
Clone ID Related Accession (Same CDS sequence)	NM_078937.4
Accession Version	NM_078937.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	patched
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_078937.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCGCG ACAGCCTCCC ACGCGTTCCG GACACACACG GCGATGTGGT CGATGAGAAA 
TTATTCTCGG ATCTTTACAT ACGCACCAGC TGGGTGGACG CCCAAGTGGC GCTCGATCAG 
ATAGATAAGG GCAAAGCGCG TGGCAGCCGC ACGGCGATCT ATCTGCGATC AGTATTCCAG 
TCCCACCTCG AAACCCTCGG CAGCTCCGTG CAAAAGCACG CGGGCAAGGT GCTATTCGTG 
GCTATCCTGG TGCTGAGCAC CTTCTGCGTC GGCCTGAAGA GCGCCCAGAT CCACTCCAAG 
GTGCACCAGC TGTGGATCCA GGAGGGCGGC CGGCTGGAGG CGGAACTGGC CTACACACAG 
AAGACGATCG GCGAGGACGA GTCGGCCACG CATCAGCTGC TCATTCAGAC GACCCACGAC 
CCGAACGCCT CCGTCCTGCA TCCGCAGGCG CTGCTTGCCC ACCTGGAGGT CCTGGTCAAG 
GCCACCGCCG TCAAGGTGCA CCTCTACGAC ACCGAATGGG GGCTGCGCGA CATGTGCAAC 
ATGCCGAGCA CGCCCTCCTT CGAGGGCATC TACTACATCG AGCAGATCCT GCGCCACCTC 
ATTCCGTGCT CGATCATCAC GCCGCTGGAC TGTTTCTGGG AGGGAAGCCA GCTGTTGGGT 
CCGGAATCAG CGGTCGTTAT ACCAGGCCTC AACCAACGAC TCCTGTGGAC CACACTGAAT 
CCCGCCTCTG TGATGCAGTA TATGAAGCAG AAGATGTCCG AGGAAAAGAT CAGCTTCGAC 
TTCGAGACCG TGGAGCAGTA CATGAAGCGT GCGGCCATTG GCAGTGGCTA CATGGAGAAG 
CCCTGCCTGA ACCCACTGAA TCCCAATTGC CCGGACACGG CACCGAACAA GAACAGCACC 
CAGCCGCCGG ATGTGGGAGC CATCCTGTCC GGAGGCTGCT ACGGTTATGC CGCGAAGCAT 
ATGCACTGGC CGGAGGAACT GATTGTGGGC GGAGCGAAGA GGAACCGCAG CGGACACTTG 
AGGAAGGCCC AGGCCCTGCA GTCGGTGGTG CAGCTGATGA CCGAGAAGGA AATGTACGAC 
CAGTGGCAGG ACAACTACAA GGTGCACCAT CTTGGATGGA CGCAGGAGAA GGCAGCGGAG 
GTTTTGAACG CCTGGCAGCG CAACTTTTCG CGGGAGGTGG AACAGCTGCT ACGTAAACAG 
TCGAGAATTG CCACCAACTA CGATATCTAC GTGTTCAGCT CGGCTGCACT GGATGACATC 
CTGGCCAAGT TCTCCCATCC CAGCGCCTTG TCCATTGTCA TCGGCGTGGC CGTCACCGTT 
TTGTATGCCT TCTGCACGCT CCTCCGCTGG AGGGACCCCG TCCGTGGACA GAGCAGTGTC 
GGCGTGGCCG GAGTTCTGCT CATGTGCTTT AGTACCGCCG CCGGATTGGG ATTGTCAGCC 
CTGCTCGGTA TCGTTTTCAA TGCCGCCAGC ACCCAGGTGG TTCCGTTTTT GGCCCTTGGT 
CTGGGCGTCG ATCACATCTT CATGCTGACC GCTGCCTATG CGGAGAGCAA TCGGCGGGAG 
CAGACCAAGC TGATTCTCAA GAAAGTGGGA CCGAGCATCC TGTTCAGTGC CTGCAGCACG 
GCAGGATCCT TCTTTGCGGC CGCCTTTATT CCGGTGCCGG CTTTGAAGGT ATTCTGTCTG 
CAGGCTGCCA TCGTAATGTG CTCCAATTTG GCAGCGGCTC TATTGGTTTT TCCGGCCATG 
ATTTCGTTGG ATCTACGGAG ACGTACCGCC GGCAGGGCGG ACATCTTCTG CTGCTGTTTT 
CCGGTGTGGA AGGAACAGCC GAAGGTGGCA CCACCGGTGC TGCCGCTGAA CAACAACAAC 
GGGCGCGGGG CCCGGCATCC GAAGAGCTGC AACAACAACA GGGTGCCGCT GCCCGCCCAG 
AATCCTCTGC TGGAACAGAG GGCAGACATC CCTGGGAGCA GTCACTCACT GGCGTCCTTC 
TCTCTGGCAA CCTTCGCCTT TCAGCACTAC ACTCCCTTCC TCATGCGCAG CTGGGTGAAG 
TTCCTGACCG TTATGGGTTT CCTGGCGGCC CTCATATCCA GCTTGTATGC CTCCACGCGC 
CTTCAGGATG GCCTGGACAT TATTGATCTG GTGCCCAAGG ACAGCAACGA GCACAAGTTC 
CTGGATGCTC AAACTCGGCT CTTTGGCTTC TACAGCATGT ATGCGGTTAC CCAGGGCAAC 
TTTGAATATC CCACCCAGCA GCAGTTGCTC AGGGACTACC ATGATTCCTT TGTGCGGGTG 
CCACATGTGA TCAAGAATGA TAATGGTGGA CTGCCGGACT TCTGGCTGCT GCTCTTCAGC 
GAGTGGCTGG GTAATCTGCA AAAGATATTC GACGAGGAAT ACCGCGACGG ACGGCTGACC 
AAGGAGTGCT GGTTCCCAAA CGCCAGCAGC GATGCCATCC TGGCCTACAA GCTAATCGTG 
CAAACCGGCC ATGTGGACAA CCCCGTGGAC AAGGAACTGG TGCTCACCAA TCGCCTGGTC 
AACAGCGATG GCATCATCAA CCAACGCGCC TTCTACAACT ATCTGTCGGC ATGGGCCACC 
AACGACGTCT TCGCCTACGG AGCTTCTCAG GGCAAATTGT ATCCGGAACC GCGCCAGTAT 
TTTCACCAAC CCAACGAGTA CGATCTTAAG ATACCCAAGA GTCTGCCATT GGTCTACGCT 
CAGATGCCCT TTTACCTCCA CGGACTAACA GATACCTCGC AGATCAAGAC CCTGATAGGT 
CATATTCGCG ACCTGAGCGT CAAGTACGAG GGCTTCGGCC TGCCCAACTA TCCATCGGGC 
ATTCCCTTCA TCTTCTGGGA GCAGTACATG ACCCTGCGCT CCTCACTGGC CATGATCCTG 
GCCTGCGTGC TACTCGCCGC CCTGGTGCTG GTCTCCCTGC TCCTGCTCTC CGTTTGGGCC 
GCCGTTCTCG TGATCCTCAG CGTTCTGGCC TCGCTGGCCC AGATCTTTGG GGCCATGACT 
CTGCTGGGCA TCAAACTCTC GGCCATTCCG GCAGTCATAC TCATCCTCAG CGTGGGCATG 
ATGCTGTGCT TCAATGTGCT GATATCACTG GGCTTCATGA CATCCGTTGG CAACCGACAG 
CGCCGCGTCC AGCTGAGCAT GCAGATGTCC CTGGGACCAC TTGTCCACGG CATGCTGACC 
TCCGGAGTGG CCGTGTTCAT GCTCTCCACG TCGCCCTTTG AGTTTGTGAT CCGGCACTTC 
TGCTGGCTTC TGCTGGTGGT CTTATGCGTT GGCGCCTGCA ACAGCCTTTT GGTGTTCCCC 
ATCCTACTGA GCATGGTGGG ACCGGAGGCG GAGCTGGTGC CGCTGGAGCA TCCAGACCGC 
ATATCCACGC CCTCTCCGCT GCCCGTGCGC AGCAGCAAGA GATCGGGCAA ATCCTATGTG 
GTGCAGGGAT CGCGATCCTC GCGAGGCAGC TGCCAGAAGT CGCATCACCA CCACCACAAA 
GACCTTAATG ATCCATCGCT GACGACGATC ACCGAGGAGC CGCAGTCGTG GAAGTCCAGC 
AACTCGTCCA TCCAGATGCC CAATGATTGG ACCTACCAGC CGCGGGAACA GCGACCCGCC 
TCCTACGCGG CCCCGCCCCC CGCCTATCAC AAGGCCGCCG CCCAGCAGCA CCACCAGCAT 
CAGGGCCCGC CCACAACGCC CCCGCCTCCC TTCCCGACGG CCTATCCGCC GGAGCTGCAG 
AGCATCGTGG TGCAGCCGGA GGTGACGGTG GAGACGACGC ACTCGGACAG CAACACCACC 
AAGGTGACGG CCACGGCCAA CATCAAGGTG GAGCTGGCCA TGCCCGGCAG GGCGGTGCGC 
AGCTATAACT TTACGAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523661.2
CDS	774..4634
Translation	MDRDSLPRVPDTHGDVVDEKLFSDLYIRTSWVDAQVALDQIDKGKARGSRTAIYLRSVFQSHLETLGSSVQKHAGKVLFVAILVLSTFCVGLKSAQIHSKVHQLWIQEGGRLEAELAYTQKTIGEDESATHQLLIQTTHDPNASVLHPQALLAHLEVLVKATAVKVHLYDTEWGLRDMCNMPSTPSFEGIYYIEQILRHLIPCSIITPLDCFWEGSQLLGPESAVVIPGLNQRLLWTTLNPASVMQYMKQKMSEEKISFDFETVEQYMKRAAIGSGYMEKPCLNPLNPNCPDTAPNKNSTQPPDVGAILSGGCYGYAAKHMHWPEELIVGGAKRNRSGHLRKAQALQSVVQLMTEKEMYDQWQDNYKVHHLGWTQEKAAEVLNAWQRNFSREVEQLLRKQSRIATNYDIYVFSSAALDDILAKFSHPSALSIVIGVAVTVLYAFCTLLRWRDPVRGQSSVGVAGVLLMCFSTAAGLGLSALLGIVFNAASTQVVPFLALGLGVDHIFMLTAAYAESNRREQTKLILKKVGPSILFSACSTAGSFFAAAFIPVPALKVFCLQAAIVMCSNLAAALLVFPAMISLDLRRRTAGRADIFCCCFPVWKEQPKVAPPVLPLNNNNGRGARHPKSCNNNRVPLPAQNPLLEQRADIPGSSHSLASFSLATFAFQHYTPFLMRSWVKFLTVMGFLAALISSLYASTRLQDGLDIIDLVPKDSNEHKFLDAQTRLFGFYSMYAVTQGNFEYPTQQQLLRDYHDSFVRVPHVIKNDNGGLPDFWLLLFSEWLGNLQKIFDEEYRDGRLTKECWFPNASSDAILAYKLIVQTGHVDNPVDKELVLTNRLVNSDGIINQRAFYNYLSAWATNDVFAYGASQGKLYPEPRQYFHQPNEYDLKIPKSLPLVYAQMPFYLHGLTDTSQIKTLIGHIRDLSVKYEGFGLPNYPSGIPFIFWEQYMTLRSSLAMILACVLLAALVLVSLLLLSVWAAVLVILSVLASLAQIFGAMTLLGIKLSAIPAVILILSVGMMLCFNVLISLGFMTSVGNRQRRVQLSMQMSLGPLVHGMLTSGVAVFMLSTSPFEFVIRHFCWLLLVVLCVGACNSLLVFPILLSMVGPEAELVPLEHPDRISTPSPLPVRSSKRSGKSYVVQGSRSSRGSCQKSHHHHHKDLNDPSLTTITEEPQSWKSSNSSIQMPNDWTYQPREQRPASYAAPPPAYHKAAAQQHHQHQGPPTTPPPPFPTAYPPELQSIVVQPEVTVETTHSDSNTTKVTATANIKVELAMPGRAVRSYNFTS

Target ORF information:

RefSeq Version	NM_078937.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster patched (ptc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078937.4

ORF Insert Sequence:

ATGGACCGCG ACAGCCTCCC ACGCGTTCCG GACACACACG GCGATGTGGT CGATGAGAAA 
TTATTCTCGG ATCTTTACAT ACGCACCAGC TGGGTGGACG CCCAAGTGGC GCTCGATCAG 
ATAGATAAGG GCAAAGCGCG TGGCAGCCGC ACGGCGATCT ATCTGCGATC AGTATTCCAG 
TCCCACCTCG AAACCCTCGG CAGCTCCGTG CAAAAGCACG CGGGCAAGGT GCTATTCGTG 
GCTATCCTGG TGCTGAGCAC CTTCTGCGTC GGCCTGAAGA GCGCCCAGAT CCACTCCAAG 
GTGCACCAGC TGTGGATCCA GGAGGGCGGC CGGCTGGAGG CGGAACTGGC CTACACACAG 
AAGACGATCG GCGAGGACGA GTCGGCCACG CATCAGCTGC TCATTCAGAC GACCCACGAC 
CCGAACGCCT CCGTCCTGCA TCCGCAGGCG CTGCTTGCCC ACCTGGAGGT CCTGGTCAAG 
GCCACCGCCG TCAAGGTGCA CCTCTACGAC ACCGAATGGG GGCTGCGCGA CATGTGCAAC 
ATGCCGAGCA CGCCCTCCTT CGAGGGCATC TACTACATCG AGCAGATCCT GCGCCACCTC 
ATTCCGTGCT CGATCATCAC GCCGCTGGAC TGTTTCTGGG AGGGAAGCCA GCTGTTGGGT 
CCGGAATCAG CGGTCGTTAT ACCAGGCCTC AACCAACGAC TCCTGTGGAC CACACTGAAT 
CCCGCCTCTG TGATGCAGTA TATGAAGCAG AAGATGTCCG AGGAAAAGAT CAGCTTCGAC 
TTCGAGACCG TGGAGCAGTA CATGAAGCGT GCGGCCATTG GCAGTGGCTA CATGGAGAAG 
CCCTGCCTGA ACCCACTGAA TCCCAATTGC CCGGACACGG CACCGAACAA GAACAGCACC 
CAGCCGCCGG ATGTGGGAGC CATCCTGTCC GGAGGCTGCT ACGGTTATGC CGCGAAGCAT 
ATGCACTGGC CGGAGGAACT GATTGTGGGC GGAGCGAAGA GGAACCGCAG CGGACACTTG 
AGGAAGGCCC AGGCCCTGCA GTCGGTGGTG CAGCTGATGA CCGAGAAGGA AATGTACGAC 
CAGTGGCAGG ACAACTACAA GGTGCACCAT CTTGGATGGA CGCAGGAGAA GGCAGCGGAG 
GTTTTGAACG CCTGGCAGCG CAACTTTTCG CGGGAGGTGG AACAGCTGCT ACGTAAACAG 
TCGAGAATTG CCACCAACTA CGATATCTAC GTGTTCAGCT CGGCTGCACT GGATGACATC 
CTGGCCAAGT TCTCCCATCC CAGCGCCTTG TCCATTGTCA TCGGCGTGGC CGTCACCGTT 
TTGTATGCCT TCTGCACGCT CCTCCGCTGG AGGGACCCCG TCCGTGGACA GAGCAGTGTC 
GGCGTGGCCG GAGTTCTGCT CATGTGCTTT AGTACCGCCG CCGGATTGGG ATTGTCAGCC 
CTGCTCGGTA TCGTTTTCAA TGCCGCCAGC ACCCAGGTGG TTCCGTTTTT GGCCCTTGGT 
CTGGGCGTCG ATCACATCTT CATGCTGACC GCTGCCTATG CGGAGAGCAA TCGGCGGGAG 
CAGACCAAGC TGATTCTCAA GAAAGTGGGA CCGAGCATCC TGTTCAGTGC CTGCAGCACG 
GCAGGATCCT TCTTTGCGGC CGCCTTTATT CCGGTGCCGG CTTTGAAGGT ATTCTGTCTG 
CAGGCTGCCA TCGTAATGTG CTCCAATTTG GCAGCGGCTC TATTGGTTTT TCCGGCCATG 
ATTTCGTTGG ATCTACGGAG ACGTACCGCC GGCAGGGCGG ACATCTTCTG CTGCTGTTTT 
CCGGTGTGGA AGGAACAGCC GAAGGTGGCA CCACCGGTGC TGCCGCTGAA CAACAACAAC 
GGGCGCGGGG CCCGGCATCC GAAGAGCTGC AACAACAACA GGGTGCCGCT GCCCGCCCAG 
AATCCTCTGC TGGAACAGAG GGCAGACATC CCTGGGAGCA GTCACTCACT GGCGTCCTTC 
TCTCTGGCAA CCTTCGCCTT TCAGCACTAC ACTCCCTTCC TCATGCGCAG CTGGGTGAAG 
TTCCTGACCG TTATGGGTTT CCTGGCGGCC CTCATATCCA GCTTGTATGC CTCCACGCGC 
CTTCAGGATG GCCTGGACAT TATTGATCTG GTGCCCAAGG ACAGCAACGA GCACAAGTTC 
CTGGATGCTC AAACTCGGCT CTTTGGCTTC TACAGCATGT ATGCGGTTAC CCAGGGCAAC 
TTTGAATATC CCACCCAGCA GCAGTTGCTC AGGGACTACC ATGATTCCTT TGTGCGGGTG 
CCACATGTGA TCAAGAATGA TAATGGTGGA CTGCCGGACT TCTGGCTGCT GCTCTTCAGC 
GAGTGGCTGG GTAATCTGCA AAAGATATTC GACGAGGAAT ACCGCGACGG ACGGCTGACC 
AAGGAGTGCT GGTTCCCAAA CGCCAGCAGC GATGCCATCC TGGCCTACAA GCTAATCGTG 
CAAACCGGCC ATGTGGACAA CCCCGTGGAC AAGGAACTGG TGCTCACCAA TCGCCTGGTC 
AACAGCGATG GCATCATCAA CCAACGCGCC TTCTACAACT ATCTGTCGGC ATGGGCCACC 
AACGACGTCT TCGCCTACGG AGCTTCTCAG GGCAAATTGT ATCCGGAACC GCGCCAGTAT 
TTTCACCAAC CCAACGAGTA CGATCTTAAG ATACCCAAGA GTCTGCCATT GGTCTACGCT 
CAGATGCCCT TTTACCTCCA CGGACTAACA GATACCTCGC AGATCAAGAC CCTGATAGGT 
CATATTCGCG ACCTGAGCGT CAAGTACGAG GGCTTCGGCC TGCCCAACTA TCCATCGGGC 
ATTCCCTTCA TCTTCTGGGA GCAGTACATG ACCCTGCGCT CCTCACTGGC CATGATCCTG 
GCCTGCGTGC TACTCGCCGC CCTGGTGCTG GTCTCCCTGC TCCTGCTCTC CGTTTGGGCC 
GCCGTTCTCG TGATCCTCAG CGTTCTGGCC TCGCTGGCCC AGATCTTTGG GGCCATGACT 
CTGCTGGGCA TCAAACTCTC GGCCATTCCG GCAGTCATAC TCATCCTCAG CGTGGGCATG 
ATGCTGTGCT TCAATGTGCT GATATCACTG GGCTTCATGA CATCCGTTGG CAACCGACAG 
CGCCGCGTCC AGCTGAGCAT GCAGATGTCC CTGGGACCAC TTGTCCACGG CATGCTGACC 
TCCGGAGTGG CCGTGTTCAT GCTCTCCACG TCGCCCTTTG AGTTTGTGAT CCGGCACTTC 
TGCTGGCTTC TGCTGGTGGT CTTATGCGTT GGCGCCTGCA ACAGCCTTTT GGTGTTCCCC 
ATCCTACTGA GCATGGTGGG ACCGGAGGCG GAGCTGGTGC CGCTGGAGCA TCCAGACCGC 
ATATCCACGC CCTCTCCGCT GCCCGTGCGC AGCAGCAAGA GATCGGGCAA ATCCTATGTG 
GTGCAGGGAT CGCGATCCTC GCGAGGCAGC TGCCAGAAGT CGCATCACCA CCACCACAAA 
GACCTTAATG ATCCATCGCT GACGACGATC ACCGAGGAGC CGCAGTCGTG GAAGTCCAGC 
AACTCGTCCA TCCAGATGCC CAATGATTGG ACCTACCAGC CGCGGGAACA GCGACCCGCC 
TCCTACGCGG CCCCGCCCCC CGCCTATCAC AAGGCCGCCG CCCAGCAGCA CCACCAGCAT 
CAGGGCCCGC CCACAACGCC CCCGCCTCCC TTCCCGACGG CCTATCCGCC GGAGCTGCAG 
AGCATCGTGG TGCAGCCGGA GGTGACGGTG GAGACGACGC ACTCGGACAG CAACACCACC 
AAGGTGACGG CCACGGCCAA CATCAAGGTG GAGCTGGCCA TGCCCGGCAG GGCGGTGCGC 
AGCTATAACT TTACGAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ptc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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