pnut cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pnut
Entrez Gene ID	35801
Full Name	peanut
Synonyms	CG8705,Dmel\CG8705,E(sina)44C,E(sina)8,PNUT,Peanut,Pnut,anon-EST:Liang-2.18,clone 2.18,l(2)02502
General protein information	Preferred Names peanut Names CG8705 gene product from transcript CG8705-RB CG8705-PA CG8705-PB enhancer of seven in absentia 8 pnut-PA pnut-PB septin septin 3 septin[Pnut]
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44C2-44C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_165597.2	NP_724659.1	peanut, isoform B
NM_057716.4	NP_477064.1	peanut, isoform A

Pathways from BioSystems

Homologies

	SEPT7	XP_002803370.1
Pan troglodytes (chimpanzee)	SEPT7	NP_001009103.2
Canis lupus familiaris (dog)	SEPT7	XP_532516.4
Gallus gallus (chicken)	SEPT7	NP_001012577.1
Danio rerio (zebrafish)	hm:zeh0351	NP_001242958.1
Magnaporthe oryzae (rice blast fungus)	MGG_01521	XP_003714495.1
Mus musculus (house mouse)	Sept7	NP_033989.2
Kluyveromyces lactis	KLLA0D04114g	XP_453247.1
Homo sapiens (human)	SEPT7	NP_001779.3
Rattus norvegicus (Norway rat)	Sept7	NP_001107212.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007596	XP_308277.4
Caenorhabditis elegans (roundworm)	unc-59	NP_493388.1
Bos taurus (cattle)	SEPT7	NP_001001168.1
Drosophila melanogaster (fruit fly)	pnut	NP_477064.1
Saccharomyces cerevisiae (baker's yeast)	CDC3	NP_013418.2
Eremothecium gossypii	AGOS_AFR111C	NP_985658.2
Schizosaccharomyces pombe (fission yeast)	spn1	NP_594754.1
Neurospora crassa	NCU08297	XP_962510.2
Xenopus tropicalis (tropical clawed frog)	sept7	XP_002939459.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

No oogenesis violations were observed in females with decreased peanut gene expression or an absence of the Pnut protein in the ovary generative cells
Title: [Analysis of Phenotypic Manifestation of peanut Gene Expression Suppression by RNAi in Drosophila Oogenesis].

These results show that the interaction of Orc6 with the septin complex is dependent on the coiled-coil domain of Pnut, and suggest an active role for Orc6 in septin complex function.
Title: Drosophila Orc6 facilitates GTPase activity and filament formation of the septin complex.

anillin and Peanut are involved in pseudocleavage furrow ingression in syncytial embryos, a process that is regulated by Ran
Title: Anillin-mediated targeting of peanut to pseudocleavage furrows is regulated by the GTPase Ran.

Peanut is ubiquinated by Parkin and has a role in the neural cell death of autosomal recessive juvenile parkinsonism.
Title: Drosophila melanogaster Parkin ubiquitinates peanut and septin1 as an E3 ubiquitin-protein ligase.

The following pnut gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pnut cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09271	NM_165597.2 Latest version!	Drosophila melanogaster peanut (pnut), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa09271	NM_057716.4 Latest version!	Drosophila melanogaster peanut (pnut), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pnut ( NM_165597.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724659.1 Drosophila melanogaster peanut (pnut), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09271
Clone ID Related Accession (Same CDS sequence)	NM_165597.2 , NM_057716.4
Accession Version	NM_165597.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	peanut, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165597.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATAGTC CTCGCTCGAA CGCGGTTAAT GGCGGCAGCG GCGGCGCCAT CTCCGCTCTG 
CCCAGCACTC TGGCCCAACT GGCTCTGCGC GACAAGCAGC AGGCTGCGTC GGCATCGGCG 
TCCTCTGCCA CGAACGGCAG CAGCGGCAGC GAGTCCTTGG TGGGCGTCGG TGGACGACCG 
CCCAATCAGC CGCCCAGTGT GCCAGTGGCC GCCAGTGGCA AGCTGGACAC CAGCGGCGGA 
GGCGCCTCCA ACGGCGACTC GAACAAACTG ACCCACGATC TGCAGGAGAA GGAGCACCAG 
CAGGCGCAGA AGCCACAGAA GCCACCGCTC CCGGTGCGCC AGAAGCCCAT GGAGATCGCC 
GGCTATGTGG GCTTCGCCAA CCTGCCCAAT CAGGTCTATC GCAAGGCCGT CAAGCGGGGA 
TTCGAGTTCA CTCTGATGGT GGTGGGCGCC AGTGGGCTGG GCAAGTCGAC GCTGATCAAC 
TCCATGTTCC TGTCGGACAT CTACAACGCG GAACAGTATC CGGGCCCCTC GCTGCGCAAA 
AAGAAGACCG TGGCCGTGGA GGCCACCAAA GTAATGCTCA AGGAGAACGG CGTCAATCTG 
ACACTCACGG TGGTGGACAC ACCCGGATTC GGTGATGCCG TTGACAACAG CAACTGCTGG 
GTGCCCATAC TGGAGTACGT GGATAGCAAG TACGAGGAGT ACCTGACAGC CGAGTCGCGG 
GTGTACCGAA AAACCATTTC GGACAGCCGG GTGCACTGCT GCCTCTACTT CATAGCGCCA 
TCGGGACACG GACTCCTGCC GCTGGACATT GCCTGCATGC AGAGCCTGTC GGACAAGGTA 
AATCTGGTGC CGGTGATCGC CAAGGCGGAC ACCATGACGC CCGACGAGGT GCATCTATTT 
AAGAAGCAGA TCCTTAACGA GATCGCCCAG CACAAGATCA AGATCTACGA CTTCCCCGCC 
ACGCTGGAGG ATGCGGCCGA GGAGGCCAAA ACCACGCAGA ATTTGCGCAG CCGAGTGCCA 
TTCGCGGTGG TGGGTGCCAA CACCATCATC GAGCAGGACG GAAAGAAGGT CCGGGGTAGG 
CGTTATCCCT GGGGCCTGGT GGAGGTGGAG AACCTGACGC ATTGCGACTT TATAGCGCTG 
CGCAACATGG TCATACGCAC GCACCTTCAG GACCTCAAGG ACGTGACGAA CAACGTCCAC 
TACGAGAACT ACCGCTGCCG GAAGCTATCC GAGCTGGGAT TGGTGGACGG CAAGGCCAGG 
CTGTCCAACA AGAACCCACT CACCCAGATG GAGGAGGAAA AGCGGGAGCA CGAGCAGAAG 
ATGAAGAAGA TGGAGGCCGA GATGGAGCAG GTGTTTGACA TGAAGGTCAA GGAGAAGATG 
CAGAAGCTCA GGGACTCGGA GCTGGAGCTG GCGCGGCGCC ACGAGGAGCG CAAGAAGGCG 
CTGGAGCTGC AGATCCGGGA GCTGGAGGAG AAGAGACGCG AGTTCGAGCG CGAGAAGAAG 
GAGTGGGAGG ATGTCAACCA CGTGACGCTC GAGGAGCTCA AGCGACGCAG CCTGGGGGCC 
AACAGCAGCA CGGACAATGT GGATGGCAAG AAGGAGAAGA AAAAGAAGGG TCTGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724659.1
CDS	787..2406
Translation	MNSPRSNAVNGGSGGAISALPSTLAQLALRDKQQAASASASSATNGSSGSESLVGVGGRPPNQPPSVPVAASGKLDTSGGGASNGDSNKLTHDLQEKEHQQAQKPQKPPLPVRQKPMEIAGYVGFANLPNQVYRKAVKRGFEFTLMVVGASGLGKSTLINSMFLSDIYNAEQYPGPSLRKKKTVAVEATKVMLKENGVNLTLTVVDTPGFGDAVDNSNCWVPILEYVDSKYEEYLTAESRVYRKTISDSRVHCCLYFIAPSGHGLLPLDIACMQSLSDKVNLVPVIAKADTMTPDEVHLFKKQILNEIAQHKIKIYDFPATLEDAAEEAKTTQNLRSRVPFAVVGANTIIEQDGKKVRGRRYPWGLVEVENLTHCDFIALRNMVIRTHLQDLKDVTNNVHYENYRCRKLSELGLVDGKARLSNKNPLTQMEEEKREHEQKMKKMEAEMEQVFDMKVKEKMQKLRDSELELARRHEERKKALELQIRELEEKRREFEREKKEWEDVNHVTLEELKRRSLGANSSTDNVDGKKEKKKKGLF

Target ORF information:

RefSeq Version	NM_165597.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster peanut (pnut), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165597.2

ORF Insert Sequence:

ATGAATAGTC CTCGCTCGAA CGCGGTTAAT GGCGGCAGCG GCGGCGCCAT CTCCGCTCTG 
CCCAGCACTC TGGCCCAACT GGCTCTGCGC GACAAGCAGC AGGCTGCGTC GGCATCGGCG 
TCCTCTGCCA CGAACGGCAG CAGCGGCAGC GAGTCCTTGG TGGGCGTCGG TGGACGACCG 
CCCAATCAGC CGCCCAGTGT GCCAGTGGCC GCCAGTGGCA AGCTGGACAC CAGCGGCGGA 
GGCGCCTCCA ACGGCGACTC GAACAAACTG ACCCACGATC TGCAGGAGAA GGAGCACCAG 
CAGGCGCAGA AGCCACAGAA GCCACCGCTC CCGGTGCGCC AGAAGCCCAT GGAGATCGCC 
GGCTATGTGG GCTTCGCCAA CCTGCCCAAT CAGGTCTATC GCAAGGCCGT CAAGCGGGGA 
TTCGAGTTCA CTCTGATGGT GGTGGGCGCC AGTGGGCTGG GCAAGTCGAC GCTGATCAAC 
TCCATGTTCC TGTCGGACAT CTACAACGCG GAACAGTATC CGGGCCCCTC GCTGCGCAAA 
AAGAAGACCG TGGCCGTGGA GGCCACCAAA GTAATGCTCA AGGAGAACGG CGTCAATCTG 
ACACTCACGG TGGTGGACAC ACCCGGATTC GGTGATGCCG TTGACAACAG CAACTGCTGG 
GTGCCCATAC TGGAGTACGT GGATAGCAAG TACGAGGAGT ACCTGACAGC CGAGTCGCGG 
GTGTACCGAA AAACCATTTC GGACAGCCGG GTGCACTGCT GCCTCTACTT CATAGCGCCA 
TCGGGACACG GACTCCTGCC GCTGGACATT GCCTGCATGC AGAGCCTGTC GGACAAGGTA 
AATCTGGTGC CGGTGATCGC CAAGGCGGAC ACCATGACGC CCGACGAGGT GCATCTATTT 
AAGAAGCAGA TCCTTAACGA GATCGCCCAG CACAAGATCA AGATCTACGA CTTCCCCGCC 
ACGCTGGAGG ATGCGGCCGA GGAGGCCAAA ACCACGCAGA ATTTGCGCAG CCGAGTGCCA 
TTCGCGGTGG TGGGTGCCAA CACCATCATC GAGCAGGACG GAAAGAAGGT CCGGGGTAGG 
CGTTATCCCT GGGGCCTGGT GGAGGTGGAG AACCTGACGC ATTGCGACTT TATAGCGCTG 
CGCAACATGG TCATACGCAC GCACCTTCAG GACCTCAAGG ACGTGACGAA CAACGTCCAC 
TACGAGAACT ACCGCTGCCG GAAGCTATCC GAGCTGGGAT TGGTGGACGG CAAGGCCAGG 
CTGTCCAACA AGAACCCACT CACCCAGATG GAGGAGGAAA AGCGGGAGCA CGAGCAGAAG 
ATGAAGAAGA TGGAGGCCGA GATGGAGCAG GTGTTTGACA TGAAGGTCAA GGAGAAGATG 
CAGAAGCTCA GGGACTCGGA GCTGGAGCTG GCGCGGCGCC ACGAGGAGCG CAAGAAGGCG 
CTGGAGCTGC AGATCCGGGA GCTGGAGGAG AAGAGACGCG AGTTCGAGCG CGAGAAGAAG 
GAGTGGGAGG ATGTCAACCA CGTGACGCTC GAGGAGCTCA AGCGACGCAG CCTGGGGGCC 
AACAGCAGCA CGGACAATGT GGATGGCAAG AAGGAGAAGA AAAAGAAGGG TCTGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pnut ( NM_057716.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477064.1 Drosophila melanogaster peanut (pnut), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09271
Clone ID Related Accession (Same CDS sequence)	NM_165597.2 , NM_057716.4
Accession Version	NM_057716.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	peanut, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057716.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATAGTC CTCGCTCGAA CGCGGTTAAT GGCGGCAGCG GCGGCGCCAT CTCCGCTCTG 
CCCAGCACTC TGGCCCAACT GGCTCTGCGC GACAAGCAGC AGGCTGCGTC GGCATCGGCG 
TCCTCTGCCA CGAACGGCAG CAGCGGCAGC GAGTCCTTGG TGGGCGTCGG TGGACGACCG 
CCCAATCAGC CGCCCAGTGT GCCAGTGGCC GCCAGTGGCA AGCTGGACAC CAGCGGCGGA 
GGCGCCTCCA ACGGCGACTC GAACAAACTG ACCCACGATC TGCAGGAGAA GGAGCACCAG 
CAGGCGCAGA AGCCACAGAA GCCACCGCTC CCGGTGCGCC AGAAGCCCAT GGAGATCGCC 
GGCTATGTGG GCTTCGCCAA CCTGCCCAAT CAGGTCTATC GCAAGGCCGT CAAGCGGGGA 
TTCGAGTTCA CTCTGATGGT GGTGGGCGCC AGTGGGCTGG GCAAGTCGAC GCTGATCAAC 
TCCATGTTCC TGTCGGACAT CTACAACGCG GAACAGTATC CGGGCCCCTC GCTGCGCAAA 
AAGAAGACCG TGGCCGTGGA GGCCACCAAA GTAATGCTCA AGGAGAACGG CGTCAATCTG 
ACACTCACGG TGGTGGACAC ACCCGGATTC GGTGATGCCG TTGACAACAG CAACTGCTGG 
GTGCCCATAC TGGAGTACGT GGATAGCAAG TACGAGGAGT ACCTGACAGC CGAGTCGCGG 
GTGTACCGAA AAACCATTTC GGACAGCCGG GTGCACTGCT GCCTCTACTT CATAGCGCCA 
TCGGGACACG GACTCCTGCC GCTGGACATT GCCTGCATGC AGAGCCTGTC GGACAAGGTA 
AATCTGGTGC CGGTGATCGC CAAGGCGGAC ACCATGACGC CCGACGAGGT GCATCTATTT 
AAGAAGCAGA TCCTTAACGA GATCGCCCAG CACAAGATCA AGATCTACGA CTTCCCCGCC 
ACGCTGGAGG ATGCGGCCGA GGAGGCCAAA ACCACGCAGA ATTTGCGCAG CCGAGTGCCA 
TTCGCGGTGG TGGGTGCCAA CACCATCATC GAGCAGGACG GAAAGAAGGT CCGGGGTAGG 
CGTTATCCCT GGGGCCTGGT GGAGGTGGAG AACCTGACGC ATTGCGACTT TATAGCGCTG 
CGCAACATGG TCATACGCAC GCACCTTCAG GACCTCAAGG ACGTGACGAA CAACGTCCAC 
TACGAGAACT ACCGCTGCCG GAAGCTATCC GAGCTGGGAT TGGTGGACGG CAAGGCCAGG 
CTGTCCAACA AGAACCCACT CACCCAGATG GAGGAGGAAA AGCGGGAGCA CGAGCAGAAG 
ATGAAGAAGA TGGAGGCCGA GATGGAGCAG GTGTTTGACA TGAAGGTCAA GGAGAAGATG 
CAGAAGCTCA GGGACTCGGA GCTGGAGCTG GCGCGGCGCC ACGAGGAGCG CAAGAAGGCG 
CTGGAGCTGC AGATCCGGGA GCTGGAGGAG AAGAGACGCG AGTTCGAGCG CGAGAAGAAG 
GAGTGGGAGG ATGTCAACCA CGTGACGCTC GAGGAGCTCA AGCGACGCAG CCTGGGGGCC 
AACAGCAGCA CGGACAATGT GGATGGCAAG AAGGAGAAGA AAAAGAAGGG TCTGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477064.1
CDS	205..1824
Translation	MNSPRSNAVNGGSGGAISALPSTLAQLALRDKQQAASASASSATNGSSGSESLVGVGGRPPNQPPSVPVAASGKLDTSGGGASNGDSNKLTHDLQEKEHQQAQKPQKPPLPVRQKPMEIAGYVGFANLPNQVYRKAVKRGFEFTLMVVGASGLGKSTLINSMFLSDIYNAEQYPGPSLRKKKTVAVEATKVMLKENGVNLTLTVVDTPGFGDAVDNSNCWVPILEYVDSKYEEYLTAESRVYRKTISDSRVHCCLYFIAPSGHGLLPLDIACMQSLSDKVNLVPVIAKADTMTPDEVHLFKKQILNEIAQHKIKIYDFPATLEDAAEEAKTTQNLRSRVPFAVVGANTIIEQDGKKVRGRRYPWGLVEVENLTHCDFIALRNMVIRTHLQDLKDVTNNVHYENYRCRKLSELGLVDGKARLSNKNPLTQMEEEKREHEQKMKKMEAEMEQVFDMKVKEKMQKLRDSELELARRHEERKKALELQIRELEEKRREFEREKKEWEDVNHVTLEELKRRSLGANSSTDNVDGKKEKKKKGLF

Target ORF information:

RefSeq Version	NM_057716.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster peanut (pnut), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057716.4

ORF Insert Sequence:

ATGAATAGTC CTCGCTCGAA CGCGGTTAAT GGCGGCAGCG GCGGCGCCAT CTCCGCTCTG 
CCCAGCACTC TGGCCCAACT GGCTCTGCGC GACAAGCAGC AGGCTGCGTC GGCATCGGCG 
TCCTCTGCCA CGAACGGCAG CAGCGGCAGC GAGTCCTTGG TGGGCGTCGG TGGACGACCG 
CCCAATCAGC CGCCCAGTGT GCCAGTGGCC GCCAGTGGCA AGCTGGACAC CAGCGGCGGA 
GGCGCCTCCA ACGGCGACTC GAACAAACTG ACCCACGATC TGCAGGAGAA GGAGCACCAG 
CAGGCGCAGA AGCCACAGAA GCCACCGCTC CCGGTGCGCC AGAAGCCCAT GGAGATCGCC 
GGCTATGTGG GCTTCGCCAA CCTGCCCAAT CAGGTCTATC GCAAGGCCGT CAAGCGGGGA 
TTCGAGTTCA CTCTGATGGT GGTGGGCGCC AGTGGGCTGG GCAAGTCGAC GCTGATCAAC 
TCCATGTTCC TGTCGGACAT CTACAACGCG GAACAGTATC CGGGCCCCTC GCTGCGCAAA 
AAGAAGACCG TGGCCGTGGA GGCCACCAAA GTAATGCTCA AGGAGAACGG CGTCAATCTG 
ACACTCACGG TGGTGGACAC ACCCGGATTC GGTGATGCCG TTGACAACAG CAACTGCTGG 
GTGCCCATAC TGGAGTACGT GGATAGCAAG TACGAGGAGT ACCTGACAGC CGAGTCGCGG 
GTGTACCGAA AAACCATTTC GGACAGCCGG GTGCACTGCT GCCTCTACTT CATAGCGCCA 
TCGGGACACG GACTCCTGCC GCTGGACATT GCCTGCATGC AGAGCCTGTC GGACAAGGTA 
AATCTGGTGC CGGTGATCGC CAAGGCGGAC ACCATGACGC CCGACGAGGT GCATCTATTT 
AAGAAGCAGA TCCTTAACGA GATCGCCCAG CACAAGATCA AGATCTACGA CTTCCCCGCC 
ACGCTGGAGG ATGCGGCCGA GGAGGCCAAA ACCACGCAGA ATTTGCGCAG CCGAGTGCCA 
TTCGCGGTGG TGGGTGCCAA CACCATCATC GAGCAGGACG GAAAGAAGGT CCGGGGTAGG 
CGTTATCCCT GGGGCCTGGT GGAGGTGGAG AACCTGACGC ATTGCGACTT TATAGCGCTG 
CGCAACATGG TCATACGCAC GCACCTTCAG GACCTCAAGG ACGTGACGAA CAACGTCCAC 
TACGAGAACT ACCGCTGCCG GAAGCTATCC GAGCTGGGAT TGGTGGACGG CAAGGCCAGG 
CTGTCCAACA AGAACCCACT CACCCAGATG GAGGAGGAAA AGCGGGAGCA CGAGCAGAAG 
ATGAAGAAGA TGGAGGCCGA GATGGAGCAG GTGTTTGACA TGAAGGTCAA GGAGAAGATG 
CAGAAGCTCA GGGACTCGGA GCTGGAGCTG GCGCGGCGCC ACGAGGAGCG CAAGAAGGCG 
CTGGAGCTGC AGATCCGGGA GCTGGAGGAG AAGAGACGCG AGTTCGAGCG CGAGAAGAAG 
GAGTGGGAGG ATGTCAACCA CGTGACGCTC GAGGAGCTCA AGCGACGCAG CCTGGGGGCC 
AACAGCAGCA CGGACAATGT GGATGGCAAG AAGGAGAAGA AAAAGAAGGG TCTGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pnut cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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