Mlh1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mlh1
Entrez Gene ID	35796
Full Name	CG11482 gene product from transcript CG11482-RB
Synonyms	CG11482,Dmel\CG11482,dmlh-1,dmlh1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44B8-44B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_057674.3	NP_477022.1	Mlh1, isoform A
NM_001273870.1	NP_001260799.1	Mlh1, isoform B

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mlh1	NP_112315.1
Drosophila melanogaster (fruit fly)	Mlh1	NP_477022.1
Magnaporthe oryzae (rice blast fungus)	MGG_07121	XP_003715311.1
Oryza sativa (rice)	Os01g0958900	NP_001045457.1
Xenopus tropicalis (tropical clawed frog)	mlh1	XP_002933455.2
Pan troglodytes (chimpanzee)	MLH1	XP_001170433.1
Canis lupus familiaris (dog)	MLH1	XP_534219.2
Danio rerio (zebrafish)	mlh1	NP_956953.1
Schizosaccharomyces pombe (fission yeast)	mlh1	NP_596199.1
Neurospora crassa	NCU08309	XP_962522.1
Gallus gallus (chicken)	MLH1	XP_418828.1
Saccharomyces cerevisiae (baker's yeast)	MLH1	NP_013890.1
Kluyveromyces lactis	KLLA0D09955g	XP_453504.1
Bos taurus (cattle)	MLH1	NP_001069462.2
Mus musculus (house mouse)	Mlh1	NP_081086.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012192	XP_320342.4
Homo sapiens (human)	MLH1	NP_000240.1
Eremothecium gossypii	AGOS_AFL199C	NP_985351.1
Arabidopsis thaliana (thale cress)	MLH1	NP_567345.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mlh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mlh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09266	NM_057674.3 Latest version!	Drosophila melanogaster Mlh1, transcript variant A (Mlh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12153	NM_001273870.1 Latest version!	Drosophila melanogaster Mlh1, transcript variant B (Mlh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mlh1 ( NM_057674.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477022.1 Drosophila melanogaster Mlh1, transcript variant A (Mlh1), mRNA.

Price & Availability↑

CloneID	OFa09266
Clone ID Related Accession (Same CDS sequence)	NM_057674.3
Accession Version	NM_057674.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Mlh1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057674.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAAT ATTTGCAGCC TGGAGTCATC CGGAAGCTGG ACGAGGTGGT GGTGAACCGC 
ATAGCCGCCG GCGAGATTAT TCAGCGACCC GCCAACGCCC TAAAAGAGCT GCTCGAGAAC 
AGTTTGGACG CCCAATCGAC CCACATCCAG GTGCAGGTGA AGGCCGGTGG GCTGAAGCTG 
CTTCAAATCC AGGACAATGG CACGGGGATA CGACGCGAGG ATCTGGCCAT CGTCTGCGAG 
CGGTTCACCA CCTCCAAGCT GACCCGCTTC GAGGATTTGT CCCAGATAGC AACATTTGGC 
TTCAGAGGAG AGGCCTTGGC CAGCATTAGT CATGTGGCGC ACCTAAGCAT CCAGACCAAG 
ACGGCTAAGG AGAAGTGCGG CTACAAGGCC ACCTACGCGG ATGGCAAGCT CCAGGGTCAG 
CCGAAGCCGT GTGCCGGAAA CCAAGGCACG ATCATTTGCA TAGAGGATCT GTTCTACAAC 
ATGCCGCAAA GGAGGCAGGC ACTCCGTTCG CCGGCCGAGG AGTTCCAGAG ATTGTCCGAG 
GTTCTGGCTA GGTACGCTGT CCACAATCCA AGGGTGGGCT TCACTCTCCG CAAGCAGGGA 
GACGCGCAAC CTGCCCTGCG GACACCAGTG GCCAGTTCCC GATCCGAAAA CATCCGCATC 
ATTTACGGGG CAGCAATTTC CAAGGAGCTG CTGGAGTTTA GCCACCGCGA TGAGGTTTAC 
AAGTTCGAGG CCGAGTGTCT GATCACTCAG GTTAACTATT CGGCCAAAAA GTGTCAGATG 
CTGCTGTTCA TCAACCAGCG ACTGGTGGAG TCCACAGCTC TCAGGACCTC CGTGGACTCG 
ATTTATGCCA CATACCTGCC CCGTGGCCAC CATCCTTTCG TATATATGAG CCTGACGCTG 
CCGCCACAGA ACTTGGACGT GAATGTCCAT CCCACCAAGC ATGAGGTGCA CTTTCTCTAC 
CAGGAAGAGA TTGTGGATAG CATTAAGCAG CAGGTTGAAG CCCGACTCCT AGGCAGCAAT 
GCCACGAGGA CGTTCTACAA ACAGCTAAGG CTACCAGGAG CCCCCGACCT GGATGAAACC 
CAGTTGGCTG ATAAAACGCA ACGCATTTAT CCCAAGGAGA TGGTGCGTAC TGACTCCACA 
GAGCAAAAGC TGGATAAGTT TTTGGCGCCT CTGGTCAAAA GTGACTCAGG CGTATCCTCC 
AGTTCCTCCC AGGAAGCGTC TCGTCTGCCA GAAGAGAGTT TTCGCGTTAC AGCGGCAAAA 
AAATCACGAG AAGTGCGTTT GAGCAGCGTG CTAGACATGA GGAAGCGCGT GGAGCGGCAG 
TGCAGCGTTC AGCTGAGGAG CACGCTCAAA AACTTGGTCT ATGTGGGCTG TGTGGATGAG 
AGGCGAGCCC TGTTCCAGCA TGAAACGCGC CTTTATATGT GCAACACACG GTCGTTTAGT 
GAGGAACTGT TCTATCAGCG GATGATTTAC GAGTTTCAAA ACTGCTCGGA AATCACAATA 
AGTCCGCCAT TGCCTCTCAA GGAGCTGCTA ATCTTGTCGC TGGAAAGCGA AGCCGCCGGC 
TGGACGCCCG AAGACGGGGA TAAGGCTGAG CTGGCGGACG GTGCTGCGGA CATTCTTCTG 
AAAAAGGCGC CCATCATGAG AGAGTATTTC GGGCTGCGCA TCTCGGAGGA TGGAATGCTC 
GAATCTCTGC CCAGTTTGCT GCACCAGCAT CGACCTTGTG TGGCCCACCT GCCTGTTTAC 
CTGCTCCGCT TGGCCACCGA AGTGGACTGG GAGCAGGAGA CACGATGCTT CGAGACATTC 
TGTAGGGAAA CTGCTCGATT TTACGCGCAA TTGGATTGGC GGGAAGGTGC GACTGCGGGC 
TTTTCCAGAT GGACAATGGA ACACGTTCTA TTTCCTGCCT TTAAAAAATA TCTTCTGCCA 
CCACCACGCA TCAAGGATCA GATCTACGAG CTCACTAACC TGCCCACTCT GTACAAGGTG 
TTCGAGCGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477022.1
CDS	20..2014
Translation	MAEYLQPGVIRKLDEVVVNRIAAGEIIQRPANALKELLENSLDAQSTHIQVQVKAGGLKLLQIQDNGTGIRREDLAIVCERFTTSKLTRFEDLSQIATFGFRGEALASISHVAHLSIQTKTAKEKCGYKATYADGKLQGQPKPCAGNQGTIICIEDLFYNMPQRRQALRSPAEEFQRLSEVLARYAVHNPRVGFTLRKQGDAQPALRTPVASSRSENIRIIYGAAISKELLEFSHRDEVYKFEAECLITQVNYSAKKCQMLLFINQRLVESTALRTSVDSIYATYLPRGHHPFVYMSLTLPPQNLDVNVHPTKHEVHFLYQEEIVDSIKQQVEARLLGSNATRTFYKQLRLPGAPDLDETQLADKTQRIYPKEMVRTDSTEQKLDKFLAPLVKSDSGVSSSSSQEASRLPEESFRVTAAKKSREVRLSSVLDMRKRVERQCSVQLRSTLKNLVYVGCVDERRALFQHETRLYMCNTRSFSEELFYQRMIYEFQNCSEITISPPLPLKELLILSLESEAAGWTPEDGDKAELADGAADILLKKAPIMREYFGLRISEDGMLESLPSLLHQHRPCVAHLPVYLLRLATEVDWEQETRCFETFCRETARFYAQLDWREGATAGFSRWTMEHVLFPAFKKYLLPPPRIKDQIYELTNLPTLYKVFERC

Target ORF information:

RefSeq Version	NM_057674.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Mlh1, transcript variant A (Mlh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057674.3

ORF Insert Sequence:

ATGGCGGAAT ATTTGCAGCC TGGAGTCATC CGGAAGCTGG ACGAGGTGGT GGTGAACCGC 
ATAGCCGCCG GCGAGATTAT TCAGCGACCC GCCAACGCCC TAAAAGAGCT GCTCGAGAAC 
AGTTTGGACG CCCAATCGAC CCACATCCAG GTGCAGGTGA AGGCCGGTGG GCTGAAGCTG 
CTTCAAATCC AGGACAATGG CACGGGGATA CGACGCGAGG ATCTGGCCAT CGTCTGCGAG 
CGGTTCACCA CCTCCAAGCT GACCCGCTTC GAGGATTTGT CCCAGATAGC AACATTTGGC 
TTCAGAGGAG AGGCCTTGGC CAGCATTAGT CATGTGGCGC ACCTAAGCAT CCAGACCAAG 
ACGGCTAAGG AGAAGTGCGG CTACAAGGCC ACCTACGCGG ATGGCAAGCT CCAGGGTCAG 
CCGAAGCCGT GTGCCGGAAA CCAAGGCACG ATCATTTGCA TAGAGGATCT GTTCTACAAC 
ATGCCGCAAA GGAGGCAGGC ACTCCGTTCG CCGGCCGAGG AGTTCCAGAG ATTGTCCGAG 
GTTCTGGCTA GGTACGCTGT CCACAATCCA AGGGTGGGCT TCACTCTCCG CAAGCAGGGA 
GACGCGCAAC CTGCCCTGCG GACACCAGTG GCCAGTTCCC GATCCGAAAA CATCCGCATC 
ATTTACGGGG CAGCAATTTC CAAGGAGCTG CTGGAGTTTA GCCACCGCGA TGAGGTTTAC 
AAGTTCGAGG CCGAGTGTCT GATCACTCAG GTTAACTATT CGGCCAAAAA GTGTCAGATG 
CTGCTGTTCA TCAACCAGCG ACTGGTGGAG TCCACAGCTC TCAGGACCTC CGTGGACTCG 
ATTTATGCCA CATACCTGCC CCGTGGCCAC CATCCTTTCG TATATATGAG CCTGACGCTG 
CCGCCACAGA ACTTGGACGT GAATGTCCAT CCCACCAAGC ATGAGGTGCA CTTTCTCTAC 
CAGGAAGAGA TTGTGGATAG CATTAAGCAG CAGGTTGAAG CCCGACTCCT AGGCAGCAAT 
GCCACGAGGA CGTTCTACAA ACAGCTAAGG CTACCAGGAG CCCCCGACCT GGATGAAACC 
CAGTTGGCTG ATAAAACGCA ACGCATTTAT CCCAAGGAGA TGGTGCGTAC TGACTCCACA 
GAGCAAAAGC TGGATAAGTT TTTGGCGCCT CTGGTCAAAA GTGACTCAGG CGTATCCTCC 
AGTTCCTCCC AGGAAGCGTC TCGTCTGCCA GAAGAGAGTT TTCGCGTTAC AGCGGCAAAA 
AAATCACGAG AAGTGCGTTT GAGCAGCGTG CTAGACATGA GGAAGCGCGT GGAGCGGCAG 
TGCAGCGTTC AGCTGAGGAG CACGCTCAAA AACTTGGTCT ATGTGGGCTG TGTGGATGAG 
AGGCGAGCCC TGTTCCAGCA TGAAACGCGC CTTTATATGT GCAACACACG GTCGTTTAGT 
GAGGAACTGT TCTATCAGCG GATGATTTAC GAGTTTCAAA ACTGCTCGGA AATCACAATA 
AGTCCGCCAT TGCCTCTCAA GGAGCTGCTA ATCTTGTCGC TGGAAAGCGA AGCCGCCGGC 
TGGACGCCCG AAGACGGGGA TAAGGCTGAG CTGGCGGACG GTGCTGCGGA CATTCTTCTG 
AAAAAGGCGC CCATCATGAG AGAGTATTTC GGGCTGCGCA TCTCGGAGGA TGGAATGCTC 
GAATCTCTGC CCAGTTTGCT GCACCAGCAT CGACCTTGTG TGGCCCACCT GCCTGTTTAC 
CTGCTCCGCT TGGCCACCGA AGTGGACTGG GAGCAGGAGA CACGATGCTT CGAGACATTC 
TGTAGGGAAA CTGCTCGATT TTACGCGCAA TTGGATTGGC GGGAAGGTGC GACTGCGGGC 
TTTTCCAGAT GGACAATGGA ACACGTTCTA TTTCCTGCCT TTAAAAAATA TCTTCTGCCA 
CCACCACGCA TCAAGGATCA GATCTACGAG CTCACTAACC TGCCCACTCT GTACAAGGTG 
TTCGAGCGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlh1 ( NM_001273870.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260799.1 Drosophila melanogaster Mlh1, transcript variant B (Mlh1), mRNA.

Price & Availability↑

CloneID	OFa12153
Clone ID Related Accession (Same CDS sequence)	NM_001273870.1
Accession Version	NM_001273870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Mlh1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAAT ATTTGCAGCC TGGAGTCATC CGGAAGCTGG ACGAGGTGGT GGTGAACCGC 
ATAGCCGCCG GCGAGATTAT TCAGCGACCC GCCAACGCCC TAAAAGAGCT GCTCGAGAAC 
AGTTTGGACG CCCAATCGAC CCACATCCAG GTGCAGGTGA AGGCCGGTGG GCTGAAGCTG 
CTTCAAATCC AGGACAATGG CACGGGGATA CGACGCGAGG ATCTGGCCAT CGTCTGCGAG 
CGGTTCACCA CCTCCAAGCT GACCCGCTTC GAGGATTTGT CCCAGATAGC AACATTTGGC 
TTCAGAGGAG AGGCCTTGGC CAGCATTAGT CATGTGGCGC ACCTAAGCAT CCAGACCAAG 
ACGGCTAAGG AGAAGTGCGG CTACAAGGCC ACCTACGCGG ATGGCAAGCT CCAGGGTCAG 
CCGAAGCCGT GTGCCGGAAA CCAAGGCACG ATCATTTGCA TAGAGGATCT GTTCTACAAC 
ATGCCGCAAA GGAGGCAGGC ACTCCGTTCG CCGGCCGAGG AGTTCCAGAG ATTGTCCGAG 
GTTCTGGCTA GGTACGCTGT CCACAATCCA AGGGTGGGCT TCACTCTCCG CAAGCAGGGA 
GACGCGCAAC CTGCCCTGCG GACACCAGTG GCCAGTTCCC GATCCGAAAA CATCCGCATC 
ATTTACGGGG CAGCAATTTC CAAGGAGCTG CTGGAGTTTA GCCACCGCGA TGAGGTTTAC 
AAGTTCGAGG CCGAGTGTCT GATCACTCAG GTTAACTATT CGGCCAAAAA GTGTCAGATG 
CTGCTGTTCA TCAACCAGCG ACTGGTGGAG TCCACAGGTT TGACCTCCGT GGACTCGATT 
TATGCCACAT ACCTGCCCCG TGGCCACCAT CCTTTCGTAT ATATGAGCCT GACGCTGCCG 
CCACAGAACT TGGACGTGAA TGTCCATCCC ACCAAGCATG AGGTGCACTT TCTCTACCAG 
GAAGAGATTG TGGATAGCAT TAAGCAGCAG GTTGAAGCCC GACTCCTAGG CAGCAATGCC 
ACGAGGACGT TCTACAAACA GCTAAGGCTA CCAGGAGCCC CCGACCTGGA TGAAACCCAG 
TTGGCTGATA AAACGCAACG CATTTATCCC AAGGAGATGG TGCGTACTGA CTCCACAGAG 
CAAAAGCTGG ATAAGTTTTT GGCGCCTCTG GTCAAAAGTG ACTCAGGCGT ATCCTCCAGT 
TCCTCCCAGG AAGCGTCTCG TCTGCCAGAA GAGAGTTTTC GCGTTACAGC GGCAAAAAAA 
TCACGAGAAG TGCGTTTGAG CAGCGTGCTA GACATGAGGA AGCGCGTGGA GCGGCAGTGC 
AGCGTTCAGC TGAGGAGCAC GCTCAAAAAC TTGGTCTATG TGGGCTGTGT GGATGAGAGG 
CGAGCCCTGT TCCAGCATGA AACGCGCCTT TATATGTGCA ACACACGGTC GTTTAGTGAG 
GAACTGTTCT ATCAGCGGAT GATTTACGAG TTTCAAAACT GCTCGGAAAT CACAATAAGT 
CCGCCATTGC CTCTCAAGGA GCTGCTAATC TTGTCGCTGG AAAGCGAAGC CGCCGGCTGG 
ACGCCCGAAG ACGGGGATAA GGCTGAGCTG GCGGACGGTG CTGCGGACAT TCTTCTGAAA 
AAGGCGCCCA TCATGAGAGA GTATTTCGGG CTGCGCATCT CGGAGGATGG AATGCTCGAA 
TCTCTGCCCA GTTTGCTGCA CCAGCATCGA CCTTGTGTGG CCCACCTGCC TGTTTACCTG 
CTCCGCTTGG CCACCGAAGT GGACTGGGAG CAGGAGACAC GATGCTTCGA GACATTCTGT 
AGGGAAACTG CTCGATTTTA CGCGCAATTG GATTGGCGGG AAGGTGCGAC TGCGGGCTTT 
TCCAGATGGA CAATGGAACA CGTTCTATTT CCTGCCTTTA AAAAATATCT TCTGCCACCA 
CCACGCATCA AGGATCAGAT CTACGAGCTC ACTAACCTGC CCACTCTGTA CAAGGTGTTC 
GAGCGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260799.1
CDS	20..2011
Translation	MAEYLQPGVIRKLDEVVVNRIAAGEIIQRPANALKELLENSLDAQSTHIQVQVKAGGLKLLQIQDNGTGIRREDLAIVCERFTTSKLTRFEDLSQIATFGFRGEALASISHVAHLSIQTKTAKEKCGYKATYADGKLQGQPKPCAGNQGTIICIEDLFYNMPQRRQALRSPAEEFQRLSEVLARYAVHNPRVGFTLRKQGDAQPALRTPVASSRSENIRIIYGAAISKELLEFSHRDEVYKFEAECLITQVNYSAKKCQMLLFINQRLVESTGLTSVDSIYATYLPRGHHPFVYMSLTLPPQNLDVNVHPTKHEVHFLYQEEIVDSIKQQVEARLLGSNATRTFYKQLRLPGAPDLDETQLADKTQRIYPKEMVRTDSTEQKLDKFLAPLVKSDSGVSSSSSQEASRLPEESFRVTAAKKSREVRLSSVLDMRKRVERQCSVQLRSTLKNLVYVGCVDERRALFQHETRLYMCNTRSFSEELFYQRMIYEFQNCSEITISPPLPLKELLILSLESEAAGWTPEDGDKAELADGAADILLKKAPIMREYFGLRISEDGMLESLPSLLHQHRPCVAHLPVYLLRLATEVDWEQETRCFETFCRETARFYAQLDWREGATAGFSRWTMEHVLFPAFKKYLLPPPRIKDQIYELTNLPTLYKVFERC

Target ORF information:

RefSeq Version	NM_001273870.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Mlh1, transcript variant B (Mlh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273870.1

ORF Insert Sequence:

ATGGCGGAAT ATTTGCAGCC TGGAGTCATC CGGAAGCTGG ACGAGGTGGT GGTGAACCGC 
ATAGCCGCCG GCGAGATTAT TCAGCGACCC GCCAACGCCC TAAAAGAGCT GCTCGAGAAC 
AGTTTGGACG CCCAATCGAC CCACATCCAG GTGCAGGTGA AGGCCGGTGG GCTGAAGCTG 
CTTCAAATCC AGGACAATGG CACGGGGATA CGACGCGAGG ATCTGGCCAT CGTCTGCGAG 
CGGTTCACCA CCTCCAAGCT GACCCGCTTC GAGGATTTGT CCCAGATAGC AACATTTGGC 
TTCAGAGGAG AGGCCTTGGC CAGCATTAGT CATGTGGCGC ACCTAAGCAT CCAGACCAAG 
ACGGCTAAGG AGAAGTGCGG CTACAAGGCC ACCTACGCGG ATGGCAAGCT CCAGGGTCAG 
CCGAAGCCGT GTGCCGGAAA CCAAGGCACG ATCATTTGCA TAGAGGATCT GTTCTACAAC 
ATGCCGCAAA GGAGGCAGGC ACTCCGTTCG CCGGCCGAGG AGTTCCAGAG ATTGTCCGAG 
GTTCTGGCTA GGTACGCTGT CCACAATCCA AGGGTGGGCT TCACTCTCCG CAAGCAGGGA 
GACGCGCAAC CTGCCCTGCG GACACCAGTG GCCAGTTCCC GATCCGAAAA CATCCGCATC 
ATTTACGGGG CAGCAATTTC CAAGGAGCTG CTGGAGTTTA GCCACCGCGA TGAGGTTTAC 
AAGTTCGAGG CCGAGTGTCT GATCACTCAG GTTAACTATT CGGCCAAAAA GTGTCAGATG 
CTGCTGTTCA TCAACCAGCG ACTGGTGGAG TCCACAGGTT TGACCTCCGT GGACTCGATT 
TATGCCACAT ACCTGCCCCG TGGCCACCAT CCTTTCGTAT ATATGAGCCT GACGCTGCCG 
CCACAGAACT TGGACGTGAA TGTCCATCCC ACCAAGCATG AGGTGCACTT TCTCTACCAG 
GAAGAGATTG TGGATAGCAT TAAGCAGCAG GTTGAAGCCC GACTCCTAGG CAGCAATGCC 
ACGAGGACGT TCTACAAACA GCTAAGGCTA CCAGGAGCCC CCGACCTGGA TGAAACCCAG 
TTGGCTGATA AAACGCAACG CATTTATCCC AAGGAGATGG TGCGTACTGA CTCCACAGAG 
CAAAAGCTGG ATAAGTTTTT GGCGCCTCTG GTCAAAAGTG ACTCAGGCGT ATCCTCCAGT 
TCCTCCCAGG AAGCGTCTCG TCTGCCAGAA GAGAGTTTTC GCGTTACAGC GGCAAAAAAA 
TCACGAGAAG TGCGTTTGAG CAGCGTGCTA GACATGAGGA AGCGCGTGGA GCGGCAGTGC 
AGCGTTCAGC TGAGGAGCAC GCTCAAAAAC TTGGTCTATG TGGGCTGTGT GGATGAGAGG 
CGAGCCCTGT TCCAGCATGA AACGCGCCTT TATATGTGCA ACACACGGTC GTTTAGTGAG 
GAACTGTTCT ATCAGCGGAT GATTTACGAG TTTCAAAACT GCTCGGAAAT CACAATAAGT 
CCGCCATTGC CTCTCAAGGA GCTGCTAATC TTGTCGCTGG AAAGCGAAGC CGCCGGCTGG 
ACGCCCGAAG ACGGGGATAA GGCTGAGCTG GCGGACGGTG CTGCGGACAT TCTTCTGAAA 
AAGGCGCCCA TCATGAGAGA GTATTTCGGG CTGCGCATCT CGGAGGATGG AATGCTCGAA 
TCTCTGCCCA GTTTGCTGCA CCAGCATCGA CCTTGTGTGG CCCACCTGCC TGTTTACCTG 
CTCCGCTTGG CCACCGAAGT GGACTGGGAG CAGGAGACAC GATGCTTCGA GACATTCTGT 
AGGGAAACTG CTCGATTTTA CGCGCAATTG GATTGGCGGG AAGGTGCGAC TGCGGGCTTT 
TCCAGATGGA CAATGGAACA CGTTCTATTT CCTGCCTTTA AAAAATATCT TCTGCCACCA 
CCACGCATCA AGGATCAGAT CTACGAGCTC ACTAACCTGC CCACTCTGTA CAAGGTGTTC 
GAGCGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mlh1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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