Nup50 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nup50
Entrez Gene ID	35784
Full Name	Nucleoporin 50kD
Synonyms	CG2158,Dmel\CG2158,NUP50,Nup,nup50
General protein information	Preferred Names Nucleoporin 50kD Names CG2158 gene product from transcript CG2158-RA CG2158-PA Nup50-PA nuclear pore protein 50 nucleoporin 50 nucleoporin 50kD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44B2-44B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_136511.3	NP_610355.1	nucleoporin 50kD

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study analyzed genomic interactions of full-length nucleoporins Nup98, Nup50, and Nup62 and found that they predominantly interacted with active genes inside the nucleoplasm, particularly those involved in developmental regulation and the cell cycle.
Title: Nucleoporins directly stimulate expression of developmental and cell-cycle genes inside the nucleoplasm.

The following Nup50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nup50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09254	NM_136511.3 Latest version!	Drosophila melanogaster nucleoporin 50kD (Nup50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nup50 ( NM_136511.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610355.1 Drosophila melanogaster nucleoporin 50kD (Nup50), mRNA.

Price & Availability↑

CloneID	OFa09254
Clone ID Related Accession (Same CDS sequence)	NM_136511.3
Accession Version	NM_136511.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 50kD
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136511.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGGCA AGCGACAAGC CACTTCGAAC CTGAACCACG AAAACTGGGA CCTGGAGGAG 
GAACCCGAGG AGCGCGGCAC ATTCCGCACG GCCACCGAAG AGGAGCTAAA GACACGCGTT 
ATCAAGAAGG CGCGCCGCAA GATTGCCGGT GGATCGTCCG CCGCTGAAGA GGATGGCGCC 
GAAGAAAAGA CTGCAGAGCC CAAAAGTGTG TTCAGTGGCT TTTCCGGTTT CGGCAAGCCG 
GCTGCGAGCC CAGCCGCGGG TTCGCCTTTC TCCTTCCTGG CCAATGTGAC GGCACCAGCC 
ACGACCTCTA GCAGCGAGCC AAAAAAGTCT GCATTCTCGT TCGGGTTCAG CTCCAGTTCC 
AGTTCGGCCG ATCGGCCTGT CAGCACTAGC ATTTGCGGCA CCGCCAGCAC CAGCAGCACG 
GCCCCCTCTC CTTTGCCGGC CAAGGAATCA ACAAGTACCG TCGATGGCGC CAAGCCGACC 
ACCTCGATAT TTGGCAACAT ATCAGCTGCC AAGAAGGAGA GTAGCGAGGC CAAAACCAGC 
AGCAGCAGCA CTAGCCTCAC ATCCACCATG GAGACCTCCG AGTACCGTGA AAGCGTTGCT 
GACCTAAATC GGTCCGTCAT AAAATTCCTG CAGGACCAAA TGGGCAAAAG TCCCTACTGC 
ATACTGACTC CAGTGTTTAA GAACTATGAT GAGCATCTAA AGGATCTGCA AGACGAGGAG 
AGCGCCAGAA CGAACTCCAC GAAATCCAAA ACGGCACAAG CTCGTTCTCA AGAACCAGTT 
GCAAAGGTGT CCCGTGCATC GTCGCCACCA AAAGCGGCTA CTACGTTTAC GTTTGGCAAG 
CCCAGCGCGC CAATAGGCGC CAGCGTATCG CCCTTAGCCA AGAAACCCAA CTGCACCATA 
ACCAGTGGTG GAACAACCAC CACGACCGCA ACACCACTGG TCTCCTTCGG CAGTACGGCA 
TCGTTCACTG CGCCAGTGCC GTCCTCCTCC AGTATCTTCA GTTTGACAGC AAAACCCACT 
GGAGAAGCCA AATCCGATGA TACCCCTAAG TCCAGCATTT TTAGCTTTGG AGCAAAGGAT 
ACGACCACTA AAAAGGATGA GCCTAACTTC TCGGCACCGA AGACCAATGG CTTTTCCTTC 
GGACTGAAAA GTAACAACGA CGACAAGCCA AGCACCTCGT TGTTTGCGGG TTTCGGAAAA 
GCGCCTGGAG GAGCAGGAGA CGGCGCAAAG GGCTTTTCCT TCACCAACAG TGCCACGCCC 
TTCTCTCTCG GAAACATTCA TCCGCCAGCT GCAGCGGCAG CGCCTGCGGA GGAGGAAAAA 
GAAGAGGATA CGCCCCCAAA AGTCGAGTTT AAACAGGTTG TGGAGGATGA CGCTATATAC 
TCCAAGCGGT GTAAGGTGTT CATTAAGAAA GACAAGGACT TTGGCGATCG TGGCGTGGGC 
ACGTTGTACT TGAAACCTGT CAAGGACTCC GAAAAGATCC AACTGTTAGT CCGCGCCGAC 
ACCAATCTGG GCAACATCTT GGTCAACCTT ATTCTTAGCA AGGGTATACC TTGCCAGCGC 
ATGGGCAAGA ACAATGTGCT GATGGTCTGC GTGCCCACGC CAGAGGACTC CAAGGCCACA 
TCGCTGTTGC TACGCGTAAA GACCGGTGAC GAAGCCGACG ACTTGCTGGA GAAGATCAAG 
GAGCACATCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610355.1
CDS	103..1797
Translation	MAGKRQATSNLNHENWDLEEEPEERGTFRTATEEELKTRVIKKARRKIAGGSSAAEEDGAEEKTAEPKSVFSGFSGFGKPAASPAAGSPFSFLANVTAPATTSSSEPKKSAFSFGFSSSSSSADRPVSTSICGTASTSSTAPSPLPAKESTSTVDGAKPTTSIFGNISAAKKESSEAKTSSSSTSLTSTMETSEYRESVADLNRSVIKFLQDQMGKSPYCILTPVFKNYDEHLKDLQDEESARTNSTKSKTAQARSQEPVAKVSRASSPPKAATTFTFGKPSAPIGASVSPLAKKPNCTITSGGTTTTTATPLVSFGSTASFTAPVPSSSSIFSLTAKPTGEAKSDDTPKSSIFSFGAKDTTTKKDEPNFSAPKTNGFSFGLKSNNDDKPSTSLFAGFGKAPGGAGDGAKGFSFTNSATPFSLGNIHPPAAAAAPAEEEKEEDTPPKVEFKQVVEDDAIYSKRCKVFIKKDKDFGDRGVGTLYLKPVKDSEKIQLLVRADTNLGNILVNLILSKGIPCQRMGKNNVLMVCVPTPEDSKATSLLLRVKTGDEADDLLEKIKEHIK

Target ORF information:

RefSeq Version	NM_136511.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 50kD (Nup50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136511.3

ORF Insert Sequence:

ATGGCTGGCA AGCGACAAGC CACTTCGAAC CTGAACCACG AAAACTGGGA CCTGGAGGAG 
GAACCCGAGG AGCGCGGCAC ATTCCGCACG GCCACCGAAG AGGAGCTAAA GACACGCGTT 
ATCAAGAAGG CGCGCCGCAA GATTGCCGGT GGATCGTCCG CCGCTGAAGA GGATGGCGCC 
GAAGAAAAGA CTGCAGAGCC CAAAAGTGTG TTCAGTGGCT TTTCCGGTTT CGGCAAGCCG 
GCTGCGAGCC CAGCCGCGGG TTCGCCTTTC TCCTTCCTGG CCAATGTGAC GGCACCAGCC 
ACGACCTCTA GCAGCGAGCC AAAAAAGTCT GCATTCTCGT TCGGGTTCAG CTCCAGTTCC 
AGTTCGGCCG ATCGGCCTGT CAGCACTAGC ATTTGCGGCA CCGCCAGCAC CAGCAGCACG 
GCCCCCTCTC CTTTGCCGGC CAAGGAATCA ACAAGTACCG TCGATGGCGC CAAGCCGACC 
ACCTCGATAT TTGGCAACAT ATCAGCTGCC AAGAAGGAGA GTAGCGAGGC CAAAACCAGC 
AGCAGCAGCA CTAGCCTCAC ATCCACCATG GAGACCTCCG AGTACCGTGA AAGCGTTGCT 
GACCTAAATC GGTCCGTCAT AAAATTCCTG CAGGACCAAA TGGGCAAAAG TCCCTACTGC 
ATACTGACTC CAGTGTTTAA GAACTATGAT GAGCATCTAA AGGATCTGCA AGACGAGGAG 
AGCGCCAGAA CGAACTCCAC GAAATCCAAA ACGGCACAAG CTCGTTCTCA AGAACCAGTT 
GCAAAGGTGT CCCGTGCATC GTCGCCACCA AAAGCGGCTA CTACGTTTAC GTTTGGCAAG 
CCCAGCGCGC CAATAGGCGC CAGCGTATCG CCCTTAGCCA AGAAACCCAA CTGCACCATA 
ACCAGTGGTG GAACAACCAC CACGACCGCA ACACCACTGG TCTCCTTCGG CAGTACGGCA 
TCGTTCACTG CGCCAGTGCC GTCCTCCTCC AGTATCTTCA GTTTGACAGC AAAACCCACT 
GGAGAAGCCA AATCCGATGA TACCCCTAAG TCCAGCATTT TTAGCTTTGG AGCAAAGGAT 
ACGACCACTA AAAAGGATGA GCCTAACTTC TCGGCACCGA AGACCAATGG CTTTTCCTTC 
GGACTGAAAA GTAACAACGA CGACAAGCCA AGCACCTCGT TGTTTGCGGG TTTCGGAAAA 
GCGCCTGGAG GAGCAGGAGA CGGCGCAAAG GGCTTTTCCT TCACCAACAG TGCCACGCCC 
TTCTCTCTCG GAAACATTCA TCCGCCAGCT GCAGCGGCAG CGCCTGCGGA GGAGGAAAAA 
GAAGAGGATA CGCCCCCAAA AGTCGAGTTT AAACAGGTTG TGGAGGATGA CGCTATATAC 
TCCAAGCGGT GTAAGGTGTT CATTAAGAAA GACAAGGACT TTGGCGATCG TGGCGTGGGC 
ACGTTGTACT TGAAACCTGT CAAGGACTCC GAAAAGATCC AACTGTTAGT CCGCGCCGAC 
ACCAATCTGG GCAACATCTT GGTCAACCTT ATTCTTAGCA AGGGTATACC TTGCCAGCGC 
ATGGGCAAGA ACAATGTGCT GATGGTCTGC GTGCCCACGC CAGAGGACTC CAAGGCCACA 
TCGCTGTTGC TACGCGTAAA GACCGGTGAC GAAGCCGACG ACTTGCTGGA GAAGATCAAG 
GAGCACATCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nup50 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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