Nup44A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nup44A
Entrez Gene ID	35762
Full Name	Nucleoporin at 44A
Synonyms	CG8722,Dmel\CG8722,FBgn 33247,Nup44A/Seh1,Seh1,nup44A,seh1
General protein information	Preferred Names Nucleoporin at 44A Names CG8722 gene product from transcript CG8722-RB CG8722-PA CG8722-PB CG8722-PC Nup44A-PA Nup44A-PB Nup44A-PC nucleoporin 44A nucleoporin at 44A nucleoporin-44A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44A2-44A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_165583.2	NP_724638.1	nucleoporin at 44A, isoform C
NM_165582.2	NP_724637.1	nucleoporin at 44A, isoform B
NM_136499.3	NP_610343.1	nucleoporin at 44A, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	SEH1L	NP_001013455.1
Canis lupus familiaris (dog)	SEH1L	XP_547686.2
Rattus norvegicus (Norway rat)	LOC100911798	XP_006254952.1
Saccharomyces cerevisiae (baker's yeast)	SEH1	NP_011415.1
Schizosaccharomyces pombe (fission yeast)	seh1	NP_593751.1
Pan troglodytes (chimpanzee)	SEH1L	XP_003315840.1
	SEH1L	XP_002808199.1
Caenorhabditis elegans (roundworm)	npp-18	NP_499740.1
Bos taurus (cattle)	SEH1L	NP_001096559.1
Gallus gallus (chicken)	SEH1L	XP_004939771.1
Xenopus tropicalis (tropical clawed frog)	seh1l	NP_001011152.1
Danio rerio (zebrafish)	seh1l	NP_956217.2
Eremothecium gossypii	AGOS_AFL038C	NP_985510.1
Mus musculus (house mouse)	Seh1l	NP_001034177.1
Drosophila melanogaster (fruit fly)	Nup44A	NP_724638.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005174	XP_314068.2
Kluyveromyces lactis	KLLA0F06754g	XP_455386.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Like mio, seh1 has a crucial germline function during oogenesis. In both mio and seh1 mutant ovaries, a fraction of oocytes fail to maintain the meiotic cycle and develop as pseudo-nurse cells.
Title: The nucleoporin Seh1 forms a complex with Mio and serves an essential tissue-specific function in Drosophila oogenesis.

The following Nup44A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nup44A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09234	NM_136499.3 Latest version!	Drosophila melanogaster nucleoporin at 44A, transcript variant A (Nup44A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09234	NM_165582.2 Latest version!	Drosophila melanogaster nucleoporin at 44A, transcript variant B (Nup44A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09234	NM_165583.2 Latest version!	Drosophila melanogaster nucleoporin at 44A, transcript variant C (Nup44A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nup44A ( NM_165583.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724638.1 Drosophila melanogaster nucleoporin at 44A, transcript variant C (Nup44A), mRNA.

Price & Availability↑

CloneID	OFa09234
Clone ID Related Accession (Same CDS sequence)	NM_165583.2 , NM_165582.2 , NM_136499.3
Accession Version	NM_165583.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin at 44A, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165583.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGACG TGGAACCCAT TATTGCCGAC CACAAGGATG TGATACACGA CGTGGTCTTC 
GACTACTACG GCCGCAGGAT GGCAACCTGT TCCAGCGATC AGACCGTCAA GATTTGGGAC 
GAGGATGGGC AGGGCAAGTG GAATGTCACG AGCAGCTGGA AAGCGCACTC CGGCTCCATT 
TGGCGCGTTT CCTGGGCGCA CCCGGAATTT GGCCAGGTTG TGGCCACATG CTCATTTGAT 
CGCACCGCCT CCGTTTGGGA GGAGGTGATT GGCGAAAAGG TATCCTCCAC CAATACCCCC 
ACTCGCCGCT GGGTGCGCCG CACCACCCTT GTAGACTCGC GGACCAGTGT CACGGATGTG 
GAGTTTGCAC CCAAGTACCT GGGACTCCTG CTGGCCACCG CCTCCGCCGA CGGCATTATT 
CGCATCTATG AGGCCCCGGA CATTATGAAC CTCTCGCAGT GGCCCGTGCA GCACGAGATT 
AGCAACAAAT TGCCGCTGAG CTGTTTGTCC TGGAACACTT CCACTTACAT GGTCACCCAG 
CTGCTGGCGG CGGGCAGCGA CGAGGCAGCC ACGCCTACTG GCAAGGTCTT CTTATTCGCC 
TACAGCGAGA ATTCCCGAAA GTGCGTTAAA ATCGATACTG TCAATGATAT AACCGATCCG 
GTCACGGATG TGGCCTTTGC TCCAAATGCC GGACGCACCT TTCACATGCT GGCAGTGGCC 
AGCAAGGATC TTTATATTGT GAACTTGCGG GGTGTAACGG ACGCCACGGA CATCTCCAAG 
TTGGACATCC AGACCATTAA GTTTAGCGAG CACAATTGCC CAGTGTGGCG CGTCTGTTGG 
AATATGCTGG CCACGATGCT GATCTCCACG GGCGACGACG GTTGTGTGCG TCTGTGGCGA 
ATGAACTACA ACCGGCAGTG GCGCTGTGCC GCCGTCTTGA AGGCAGAGGG CAGCGGACCC 
ACCTACGAGC CTGCTCCGCC CACTCCGACA CTGGCCACTA CCGCCTCGGC CACGGCCAAG 
TTCTACAAAA AGGGCACCAT CGGCAACCAG GTGCCGTGGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724638.1
CDS	218..1282
Translation	MFDVEPIIADHKDVIHDVVFDYYGRRMATCSSDQTVKIWDEDGQGKWNVTSSWKAHSGSIWRVSWAHPEFGQVVATCSFDRTASVWEEVIGEKVSSTNTPTRRWVRRTTLVDSRTSVTDVEFAPKYLGLLLATASADGIIRIYEAPDIMNLSQWPVQHEISNKLPLSCLSWNTSTYMVTQLLAAGSDEAATPTGKVFLFAYSENSRKCVKIDTVNDITDPVTDVAFAPNAGRTFHMLAVASKDLYIVNLRGVTDATDISKLDIQTIKFSEHNCPVWRVCWNMLATMLISTGDDGCVRLWRMNYNRQWRCAAVLKAEGSGPTYEPAPPTPTLATTASATAKFYKKGTIGNQVPWH

Target ORF information:

RefSeq Version	NM_165583.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin at 44A, transcript variant C (Nup44A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165583.2

ORF Insert Sequence:

ATGTTCGACG TGGAACCCAT TATTGCCGAC CACAAGGATG TGATACACGA CGTGGTCTTC 
GACTACTACG GCCGCAGGAT GGCAACCTGT TCCAGCGATC AGACCGTCAA GATTTGGGAC 
GAGGATGGGC AGGGCAAGTG GAATGTCACG AGCAGCTGGA AAGCGCACTC CGGCTCCATT 
TGGCGCGTTT CCTGGGCGCA CCCGGAATTT GGCCAGGTTG TGGCCACATG CTCATTTGAT 
CGCACCGCCT CCGTTTGGGA GGAGGTGATT GGCGAAAAGG TATCCTCCAC CAATACCCCC 
ACTCGCCGCT GGGTGCGCCG CACCACCCTT GTAGACTCGC GGACCAGTGT CACGGATGTG 
GAGTTTGCAC CCAAGTACCT GGGACTCCTG CTGGCCACCG CCTCCGCCGA CGGCATTATT 
CGCATCTATG AGGCCCCGGA CATTATGAAC CTCTCGCAGT GGCCCGTGCA GCACGAGATT 
AGCAACAAAT TGCCGCTGAG CTGTTTGTCC TGGAACACTT CCACTTACAT GGTCACCCAG 
CTGCTGGCGG CGGGCAGCGA CGAGGCAGCC ACGCCTACTG GCAAGGTCTT CTTATTCGCC 
TACAGCGAGA ATTCCCGAAA GTGCGTTAAA ATCGATACTG TCAATGATAT AACCGATCCG 
GTCACGGATG TGGCCTTTGC TCCAAATGCC GGACGCACCT TTCACATGCT GGCAGTGGCC 
AGCAAGGATC TTTATATTGT GAACTTGCGG GGTGTAACGG ACGCCACGGA CATCTCCAAG 
TTGGACATCC AGACCATTAA GTTTAGCGAG CACAATTGCC CAGTGTGGCG CGTCTGTTGG 
AATATGCTGG CCACGATGCT GATCTCCACG GGCGACGACG GTTGTGTGCG TCTGTGGCGA 
ATGAACTACA ACCGGCAGTG GCGCTGTGCC GCCGTCTTGA AGGCAGAGGG CAGCGGACCC 
ACCTACGAGC CTGCTCCGCC CACTCCGACA CTGGCCACTA CCGCCTCGGC CACGGCCAAG 
TTCTACAAAA AGGGCACCAT CGGCAACCAG GTGCCGTGGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup44A ( NM_165582.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724637.1 Drosophila melanogaster nucleoporin at 44A, transcript variant B (Nup44A), mRNA.

Price & Availability↑

CloneID	OFa09234
Clone ID Related Accession (Same CDS sequence)	NM_165583.2 , NM_165582.2 , NM_136499.3
Accession Version	NM_165582.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin at 44A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165582.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGACG TGGAACCCAT TATTGCCGAC CACAAGGATG TGATACACGA CGTGGTCTTC 
GACTACTACG GCCGCAGGAT GGCAACCTGT TCCAGCGATC AGACCGTCAA GATTTGGGAC 
GAGGATGGGC AGGGCAAGTG GAATGTCACG AGCAGCTGGA AAGCGCACTC CGGCTCCATT 
TGGCGCGTTT CCTGGGCGCA CCCGGAATTT GGCCAGGTTG TGGCCACATG CTCATTTGAT 
CGCACCGCCT CCGTTTGGGA GGAGGTGATT GGCGAAAAGG TATCCTCCAC CAATACCCCC 
ACTCGCCGCT GGGTGCGCCG CACCACCCTT GTAGACTCGC GGACCAGTGT CACGGATGTG 
GAGTTTGCAC CCAAGTACCT GGGACTCCTG CTGGCCACCG CCTCCGCCGA CGGCATTATT 
CGCATCTATG AGGCCCCGGA CATTATGAAC CTCTCGCAGT GGCCCGTGCA GCACGAGATT 
AGCAACAAAT TGCCGCTGAG CTGTTTGTCC TGGAACACTT CCACTTACAT GGTCACCCAG 
CTGCTGGCGG CGGGCAGCGA CGAGGCAGCC ACGCCTACTG GCAAGGTCTT CTTATTCGCC 
TACAGCGAGA ATTCCCGAAA GTGCGTTAAA ATCGATACTG TCAATGATAT AACCGATCCG 
GTCACGGATG TGGCCTTTGC TCCAAATGCC GGACGCACCT TTCACATGCT GGCAGTGGCC 
AGCAAGGATC TTTATATTGT GAACTTGCGG GGTGTAACGG ACGCCACGGA CATCTCCAAG 
TTGGACATCC AGACCATTAA GTTTAGCGAG CACAATTGCC CAGTGTGGCG CGTCTGTTGG 
AATATGCTGG CCACGATGCT GATCTCCACG GGCGACGACG GTTGTGTGCG TCTGTGGCGA 
ATGAACTACA ACCGGCAGTG GCGCTGTGCC GCCGTCTTGA AGGCAGAGGG CAGCGGACCC 
ACCTACGAGC CTGCTCCGCC CACTCCGACA CTGGCCACTA CCGCCTCGGC CACGGCCAAG 
TTCTACAAAA AGGGCACCAT CGGCAACCAG GTGCCGTGGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724637.1
CDS	170..1234
Translation	MFDVEPIIADHKDVIHDVVFDYYGRRMATCSSDQTVKIWDEDGQGKWNVTSSWKAHSGSIWRVSWAHPEFGQVVATCSFDRTASVWEEVIGEKVSSTNTPTRRWVRRTTLVDSRTSVTDVEFAPKYLGLLLATASADGIIRIYEAPDIMNLSQWPVQHEISNKLPLSCLSWNTSTYMVTQLLAAGSDEAATPTGKVFLFAYSENSRKCVKIDTVNDITDPVTDVAFAPNAGRTFHMLAVASKDLYIVNLRGVTDATDISKLDIQTIKFSEHNCPVWRVCWNMLATMLISTGDDGCVRLWRMNYNRQWRCAAVLKAEGSGPTYEPAPPTPTLATTASATAKFYKKGTIGNQVPWH

Target ORF information:

RefSeq Version	NM_165582.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin at 44A, transcript variant B (Nup44A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165582.2

ORF Insert Sequence:

ATGTTCGACG TGGAACCCAT TATTGCCGAC CACAAGGATG TGATACACGA CGTGGTCTTC 
GACTACTACG GCCGCAGGAT GGCAACCTGT TCCAGCGATC AGACCGTCAA GATTTGGGAC 
GAGGATGGGC AGGGCAAGTG GAATGTCACG AGCAGCTGGA AAGCGCACTC CGGCTCCATT 
TGGCGCGTTT CCTGGGCGCA CCCGGAATTT GGCCAGGTTG TGGCCACATG CTCATTTGAT 
CGCACCGCCT CCGTTTGGGA GGAGGTGATT GGCGAAAAGG TATCCTCCAC CAATACCCCC 
ACTCGCCGCT GGGTGCGCCG CACCACCCTT GTAGACTCGC GGACCAGTGT CACGGATGTG 
GAGTTTGCAC CCAAGTACCT GGGACTCCTG CTGGCCACCG CCTCCGCCGA CGGCATTATT 
CGCATCTATG AGGCCCCGGA CATTATGAAC CTCTCGCAGT GGCCCGTGCA GCACGAGATT 
AGCAACAAAT TGCCGCTGAG CTGTTTGTCC TGGAACACTT CCACTTACAT GGTCACCCAG 
CTGCTGGCGG CGGGCAGCGA CGAGGCAGCC ACGCCTACTG GCAAGGTCTT CTTATTCGCC 
TACAGCGAGA ATTCCCGAAA GTGCGTTAAA ATCGATACTG TCAATGATAT AACCGATCCG 
GTCACGGATG TGGCCTTTGC TCCAAATGCC GGACGCACCT TTCACATGCT GGCAGTGGCC 
AGCAAGGATC TTTATATTGT GAACTTGCGG GGTGTAACGG ACGCCACGGA CATCTCCAAG 
TTGGACATCC AGACCATTAA GTTTAGCGAG CACAATTGCC CAGTGTGGCG CGTCTGTTGG 
AATATGCTGG CCACGATGCT GATCTCCACG GGCGACGACG GTTGTGTGCG TCTGTGGCGA 
ATGAACTACA ACCGGCAGTG GCGCTGTGCC GCCGTCTTGA AGGCAGAGGG CAGCGGACCC 
ACCTACGAGC CTGCTCCGCC CACTCCGACA CTGGCCACTA CCGCCTCGGC CACGGCCAAG 
TTCTACAAAA AGGGCACCAT CGGCAACCAG GTGCCGTGGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup44A ( NM_136499.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610343.1 Drosophila melanogaster nucleoporin at 44A, transcript variant A (Nup44A), mRNA.

Price & Availability↑

CloneID	OFa09234
Clone ID Related Accession (Same CDS sequence)	NM_165583.2 , NM_165582.2 , NM_136499.3
Accession Version	NM_136499.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin at 44A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136499.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGACG TGGAACCCAT TATTGCCGAC CACAAGGATG TGATACACGA CGTGGTCTTC 
GACTACTACG GCCGCAGGAT GGCAACCTGT TCCAGCGATC AGACCGTCAA GATTTGGGAC 
GAGGATGGGC AGGGCAAGTG GAATGTCACG AGCAGCTGGA AAGCGCACTC CGGCTCCATT 
TGGCGCGTTT CCTGGGCGCA CCCGGAATTT GGCCAGGTTG TGGCCACATG CTCATTTGAT 
CGCACCGCCT CCGTTTGGGA GGAGGTGATT GGCGAAAAGG TATCCTCCAC CAATACCCCC 
ACTCGCCGCT GGGTGCGCCG CACCACCCTT GTAGACTCGC GGACCAGTGT CACGGATGTG 
GAGTTTGCAC CCAAGTACCT GGGACTCCTG CTGGCCACCG CCTCCGCCGA CGGCATTATT 
CGCATCTATG AGGCCCCGGA CATTATGAAC CTCTCGCAGT GGCCCGTGCA GCACGAGATT 
AGCAACAAAT TGCCGCTGAG CTGTTTGTCC TGGAACACTT CCACTTACAT GGTCACCCAG 
CTGCTGGCGG CGGGCAGCGA CGAGGCAGCC ACGCCTACTG GCAAGGTCTT CTTATTCGCC 
TACAGCGAGA ATTCCCGAAA GTGCGTTAAA ATCGATACTG TCAATGATAT AACCGATCCG 
GTCACGGATG TGGCCTTTGC TCCAAATGCC GGACGCACCT TTCACATGCT GGCAGTGGCC 
AGCAAGGATC TTTATATTGT GAACTTGCGG GGTGTAACGG ACGCCACGGA CATCTCCAAG 
TTGGACATCC AGACCATTAA GTTTAGCGAG CACAATTGCC CAGTGTGGCG CGTCTGTTGG 
AATATGCTGG CCACGATGCT GATCTCCACG GGCGACGACG GTTGTGTGCG TCTGTGGCGA 
ATGAACTACA ACCGGCAGTG GCGCTGTGCC GCCGTCTTGA AGGCAGAGGG CAGCGGACCC 
ACCTACGAGC CTGCTCCGCC CACTCCGACA CTGGCCACTA CCGCCTCGGC CACGGCCAAG 
TTCTACAAAA AGGGCACCAT CGGCAACCAG GTGCCGTGGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610343.1
CDS	95..1159
Translation	MFDVEPIIADHKDVIHDVVFDYYGRRMATCSSDQTVKIWDEDGQGKWNVTSSWKAHSGSIWRVSWAHPEFGQVVATCSFDRTASVWEEVIGEKVSSTNTPTRRWVRRTTLVDSRTSVTDVEFAPKYLGLLLATASADGIIRIYEAPDIMNLSQWPVQHEISNKLPLSCLSWNTSTYMVTQLLAAGSDEAATPTGKVFLFAYSENSRKCVKIDTVNDITDPVTDVAFAPNAGRTFHMLAVASKDLYIVNLRGVTDATDISKLDIQTIKFSEHNCPVWRVCWNMLATMLISTGDDGCVRLWRMNYNRQWRCAAVLKAEGSGPTYEPAPPTPTLATTASATAKFYKKGTIGNQVPWH

Target ORF information:

RefSeq Version	NM_136499.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin at 44A, transcript variant A (Nup44A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136499.3

ORF Insert Sequence:

ATGTTCGACG TGGAACCCAT TATTGCCGAC CACAAGGATG TGATACACGA CGTGGTCTTC 
GACTACTACG GCCGCAGGAT GGCAACCTGT TCCAGCGATC AGACCGTCAA GATTTGGGAC 
GAGGATGGGC AGGGCAAGTG GAATGTCACG AGCAGCTGGA AAGCGCACTC CGGCTCCATT 
TGGCGCGTTT CCTGGGCGCA CCCGGAATTT GGCCAGGTTG TGGCCACATG CTCATTTGAT 
CGCACCGCCT CCGTTTGGGA GGAGGTGATT GGCGAAAAGG TATCCTCCAC CAATACCCCC 
ACTCGCCGCT GGGTGCGCCG CACCACCCTT GTAGACTCGC GGACCAGTGT CACGGATGTG 
GAGTTTGCAC CCAAGTACCT GGGACTCCTG CTGGCCACCG CCTCCGCCGA CGGCATTATT 
CGCATCTATG AGGCCCCGGA CATTATGAAC CTCTCGCAGT GGCCCGTGCA GCACGAGATT 
AGCAACAAAT TGCCGCTGAG CTGTTTGTCC TGGAACACTT CCACTTACAT GGTCACCCAG 
CTGCTGGCGG CGGGCAGCGA CGAGGCAGCC ACGCCTACTG GCAAGGTCTT CTTATTCGCC 
TACAGCGAGA ATTCCCGAAA GTGCGTTAAA ATCGATACTG TCAATGATAT AACCGATCCG 
GTCACGGATG TGGCCTTTGC TCCAAATGCC GGACGCACCT TTCACATGCT GGCAGTGGCC 
AGCAAGGATC TTTATATTGT GAACTTGCGG GGTGTAACGG ACGCCACGGA CATCTCCAAG 
TTGGACATCC AGACCATTAA GTTTAGCGAG CACAATTGCC CAGTGTGGCG CGTCTGTTGG 
AATATGCTGG CCACGATGCT GATCTCCACG GGCGACGACG GTTGTGTGCG TCTGTGGCGA 
ATGAACTACA ACCGGCAGTG GCGCTGTGCC GCCGTCTTGA AGGCAGAGGG CAGCGGACCC 
ACCTACGAGC CTGCTCCGCC CACTCCGACA CTGGCCACTA CCGCCTCGGC CACGGCCAAG 
TTCTACAAAA AGGGCACCAT CGGCAACCAG GTGCCGTGGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nup44A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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