tor cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tor
Entrez Gene ID	35717
Full Name	torso
Synonyms	CG1389,Dmel\CG1389,TOR,Tor,Torso,splc
General protein information	Preferred Names torso Names CG1389 gene product from transcript CG1389-RD CG1389-PA CG1389-PD spliced tor-PA tor-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 43E12-43E12

mRNA and Protein(s)

mRNA	Protein	Name
NM_057414.4	NP_476762.1	torso, isoform A
NM_001103742.2	NP_001097212.2	torso, isoform D

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005763	XP_556418.3
Drosophila melanogaster (fruit fly)	tor	NP_001097211.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CRL3(GCL) promotes primordial germ cell fate by mediating degradation of Torso, a receptor tyrosine kinase (RTK) and major determinant of somatic cell fate.
Title: GCL and CUL3 Control the Switch between Cell Lineages by Mediating Localized Degradation of an RTK.

Torso interacts with both Trunk and Torso-like, which cooperate to mediate dimerization and activation of Torso at the ends of the Drosophila embryo
Title: Reconstitution of Torso signaling in cultured cells suggests a role for both Trunk and Torso-like in receptor activation.

Our study revealed a novel role for Torso in the fat body with respect to regulation of insulin/TOR signaling and body size.
Title: Torso, a Drosophila receptor tyrosine kinase, plays a novel role in the larval fat body in regulating insulin signaling and body growth.

The conserved Tor metabolic sensing pathway regulates yolk catabolism.
Title: The Atg1-Tor pathway regulates yolk catabolism in Drosophila embryos.

Data suggest that Tor signaling governs Yki (Yorkie protein) action after reaching nucleus by allowing access to target genes via Hpo (Hippo protein) pathway; Tor also promotes wing growth by liberating Yki from nuclear seclusion.
Title: Scaling the Drosophila Wing: TOR-Dependent Target Gene Access by the Hippo Pathway Transducer Yorkie.

The following tor gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tor cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09188	NM_057414.4 Latest version!	Drosophila melanogaster torso (tor), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa21699	NM_001103742.2 Latest version!	Drosophila melanogaster torso (tor), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tor ( NM_057414.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476762.1 Drosophila melanogaster torso (tor), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09188
Clone ID Related Accession (Same CDS sequence)	NM_057414.4
Accession Version	NM_057414.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	torso, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057414.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTATTT TCTACGCGAA GTACGCATTT ATCTTCTGGT TTTTCGTGGG AAGCAATCAA 
GGTGAAATGT TGCTAATGGA CAAAATCTCT CACGATAAGA CGCTTCTCAA CGTCACCGCT 
TGCACCCAGA ATTGTCTGGA AAAGGGCCAG ATGGATTTCC GAAGCTGTTT AAAGGACTGC 
AGGATTAATG GAACATTTCC CGGGGCTCTG CGCAAGGTGC AGGAAAACTA CCAGATGAAC 
ATGATCTGCC GCACGGAGTC GGAAATCGTT TTCCAAATAG ATTGGGTGCA GCACAGCAGG 
GGAACCGAGC CGGCTCCAAA TGCCACCTAC ATAATCCGGG TGGATGCTGT CAAGGACGAC 
AACAAAGAAA CTGCGCTTTA CCTGTCTGAT GACAACTTTC TCATCCTGCC GGGATTGGAG 
TCCAACTCTA CCCACAACAT CACCGCCCTG GCGATGCACG GAGATGGCAG CTACTCCTTG 
ATAGCAAAGG ACCAGACCTT CGCCACCCTC ATCCGAGGCT ATCAGCCCAG CAAAATGGGA 
GCGGTGAATC TGCTGCGGTT TGTCCCCCAA CCAGACGACC TGCATCACAT TGCTGCCGAA 
ATCGAGTGGA AGCCATCGGC GGAGAGCAAC TGCTATTTCG ACATGGTGTC GTATTCAACC 
AACAGCGTGA ATATGGACGA GCCACTGGAG GTGCAGTTCC GGGATCGCAA AAAGCTGTAC 
AGGCACACGG TGGACAACTT GGAGTTTGAC AAACAGTATC ACGTTGGCGT AAGAACGGTG 
AACATAATGA ATCGACTGGA GAGCGATCTG CAGTGGCTGC CAATCGCTGT TCCAAGCTGC 
TTGGATTGGT ATCCCTATAA CTACACACTC TGCCCACCCC ATAAGCCAGA GAATCTTACT 
GTGACCCAGA AGCAGTATCT GCCAAATATT TTGGCCCTGA ACATCACCTG GGCGCGTCCC 
AGATACCTGC CGGATAACTA TACACTTCAC ATCTTTGATC TATTCAAAGG AGGTACGGAG 
CTAAACTATA CACTTGACCA AAACAGGAGC CACTTCTATG TACCCAAGAT CACGGTACTG 
GGTTCCCATT TCGAAGTACA TTTGGTGGCC CAGTCGGCAG GCGGAAAAAA CGTATCCGGT 
TTGACGTTGG ACAAGGTTCA TCGAGGTGTG TTGCTGAGCG AGGGCAACAT GGTCAAGTTG 
GTACTCTTTA TTATCGTGCC CATATGCTGC ATTTTGATGC TGTGCTCCCT GACGTTCTGC 
AGACGAAATC GTTCGGAGGT TCAGGCGCTG CAAATGGACG CTAAGGACGC GAAGGCCAGT 
GAATTTCATC TCTCCCTGAT GGACAGCAGT GGCCTGCTGG TCACCCTCTC GGCCAACGAG 
AGTCTGGAAG TAATGGACGA GCTGGAGGTG GAGCCACACT CGGTGCTCCT TCAGGATGTC 
CTCGGCGAAG GAGCCTTTGG CTTGGTGCGA CGTGGAGTTT ACAAGAAACG CCAAGTGGCC 
GTCAAGTTGC TGAAAGATGA ACCAAACGAC GAGGACGTAT ATGCGTTCAA GTGCGAAATT 
CAGATGCTCA AGGCCGTGGG CAAGCATCCA AATATTGTGG GTATCGTGGG ATACTCCACT 
CGTTTTAGCA ACCAGATGAT GTTGCTAATT GAATACTGCA GCCTTGGAAG CCTGCAGAAC 
TTTTTACGTG AGGAGTGGAA GTTCAGGCAG GAGCAAAATG CAATTGGACT TAAGAAGAAC 
CTTGAACAGA ACGTGGACAA CCGACGGTTT AACCGACTCC CTAGAAATTC CATCCATGAT 
CGCATAGAGG ATATCAACAA CTCGATGCTG TCCACTGTGG AAGAGGAGAG TGAATCGGAT 
CAGACACACT CAAGTCGATG TGAGACCTAC ACCCTCACTC GAATAACCAA TGCAGCCGAC 
AACAAGGGCT ATGGCCTGGA GGACATTGAA AACATCGGTG GGAGTTACAT TCCCAAAACC 
GCTGAAGCTC CAAAGGATCG GCCAAAACGG AAGCTGAAGC CGCAGCCCAA GAAAGACTCG 
AAGCAGGATT TCAAATCGGA CAACAAGAAG CGAATCTTTG AGAACAAGGA ATACTTTGAT 
TGCCTCGACT CATCGGATAC CAAGCCCCGA ATACCACTGA AATATGCAGA TTTGCTAGAC 
ATCGCCCAAC AGGTGGCGGT GGGAATGGAA TTTCTGGCCC AAAACAAAGT AGTGCATAGG 
GATCTGGCTG CCCGGAATGT TCTAATCTCC GTAGATCGCA GCATCAAGAT AGCAGATTTT 
GGGCTGAGTC GAGATGTGTA TCATGAGAAC GTGTACCGAA AGTCCGGAGG AAGTGGCAAG 
CTGCCCATCA AGTGGCTCGC GCTGGAGTCC CTCACCCACC AGGTGTACAC CAGTCAGAGC 
GATGTTTGGT CCTTTGGTGT GCTGCTCTAT GAGATCACCA CTCTCGGTGG AATGCCATAT 
CCGTCGGTGT CTCCCAGTGA TCTCTTGCAG CTACTGCGAC AAGGTCATCG GATGAAGCGA 
CCGGAGGGAT GTACGCAAGA AATGTTTTCC CTGATGGAAA GCTGCTGGAG CTCCGTGCCA 
TCACACAGGC CAACATTTTC CGCCCTTAAA CACAGACTTG GTGGCATGAT TTTGGCCACT 
AACGATGTTC CAGAAAGGCT GAAACAACTG CAAGCTGCAA CCGAGTCAAA ATTAAAGTCA 
TGTGACGGTC TAAACAGTAA AGTGGAGCAA GTGCCATGCG AGGAAGAGCT ATACCTAGAA 
CCTTTGAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476762.1
CDS	240..3011
Translation	MLIFYAKYAFIFWFFVGSNQGEMLLMDKISHDKTLLNVTACTQNCLEKGQMDFRSCLKDCRINGTFPGALRKVQENYQMNMICRTESEIVFQIDWVQHSRGTEPAPNATYIIRVDAVKDDNKETALYLSDDNFLILPGLESNSTHNITALAMHGDGSYSLIAKDQTFATLIRGYQPSKMGAVNLLRFVPQPDDLHHIAAEIEWKPSAESNCYFDMVSYSTNSVNMDEPLEVQFRDRKKLYRHTVDNLEFDKQYHVGVRTVNIMNRLESDLQWLPIAVPSCLDWYPYNYTLCPPHKPENLTVTQKQYLPNILALNITWARPRYLPDNYTLHIFDLFKGGTELNYTLDQNRSHFYVPKITVLGSHFEVHLVAQSAGGKNVSGLTLDKVHRGVLLSEGNMVKLVLFIIVPICCILMLCSLTFCRRNRSEVQALQMDAKDAKASEFHLSLMDSSGLLVTLSANESLEVMDELEVEPHSVLLQDVLGEGAFGLVRRGVYKKRQVAVKLLKDEPNDEDVYAFKCEIQMLKAVGKHPNIVGIVGYSTRFSNQMMLLIEYCSLGSLQNFLREEWKFRQEQNAIGLKKNLEQNVDNRRFNRLPRNSIHDRIEDINNSMLSTVEEESESDQTHSSRCETYTLTRITNAADNKGYGLEDIENIGGSYIPKTAEAPKDRPKRKLKPQPKKDSKQDFKSDNKKRIFENKEYFDCLDSSDTKPRIPLKYADLLDIAQQVAVGMEFLAQNKVVHRDLAARNVLISVDRSIKIADFGLSRDVYHENVYRKSGGSGKLPIKWLALESLTHQVYTSQSDVWSFGVLLYEITTLGGMPYPSVSPSDLLQLLRQGHRMKRPEGCTQEMFSLMESCWSSVPSHRPTFSALKHRLGGMILATNDVPERLKQLQAATESKLKSCDGLNSKVEQVPCEEELYLEPLN

Target ORF information:

RefSeq Version	NM_057414.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster torso (tor), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057414.4

ORF Insert Sequence:

ATGCTTATTT TCTACGCGAA GTACGCATTT ATCTTCTGGT TTTTCGTGGG AAGCAATCAA 
GGTGAAATGT TGCTAATGGA CAAAATCTCT CACGATAAGA CGCTTCTCAA CGTCACCGCT 
TGCACCCAGA ATTGTCTGGA AAAGGGCCAG ATGGATTTCC GAAGCTGTTT AAAGGACTGC 
AGGATTAATG GAACATTTCC CGGGGCTCTG CGCAAGGTGC AGGAAAACTA CCAGATGAAC 
ATGATCTGCC GCACGGAGTC GGAAATCGTT TTCCAAATAG ATTGGGTGCA GCACAGCAGG 
GGAACCGAGC CGGCTCCAAA TGCCACCTAC ATAATCCGGG TGGATGCTGT CAAGGACGAC 
AACAAAGAAA CTGCGCTTTA CCTGTCTGAT GACAACTTTC TCATCCTGCC GGGATTGGAG 
TCCAACTCTA CCCACAACAT CACCGCCCTG GCGATGCACG GAGATGGCAG CTACTCCTTG 
ATAGCAAAGG ACCAGACCTT CGCCACCCTC ATCCGAGGCT ATCAGCCCAG CAAAATGGGA 
GCGGTGAATC TGCTGCGGTT TGTCCCCCAA CCAGACGACC TGCATCACAT TGCTGCCGAA 
ATCGAGTGGA AGCCATCGGC GGAGAGCAAC TGCTATTTCG ACATGGTGTC GTATTCAACC 
AACAGCGTGA ATATGGACGA GCCACTGGAG GTGCAGTTCC GGGATCGCAA AAAGCTGTAC 
AGGCACACGG TGGACAACTT GGAGTTTGAC AAACAGTATC ACGTTGGCGT AAGAACGGTG 
AACATAATGA ATCGACTGGA GAGCGATCTG CAGTGGCTGC CAATCGCTGT TCCAAGCTGC 
TTGGATTGGT ATCCCTATAA CTACACACTC TGCCCACCCC ATAAGCCAGA GAATCTTACT 
GTGACCCAGA AGCAGTATCT GCCAAATATT TTGGCCCTGA ACATCACCTG GGCGCGTCCC 
AGATACCTGC CGGATAACTA TACACTTCAC ATCTTTGATC TATTCAAAGG AGGTACGGAG 
CTAAACTATA CACTTGACCA AAACAGGAGC CACTTCTATG TACCCAAGAT CACGGTACTG 
GGTTCCCATT TCGAAGTACA TTTGGTGGCC CAGTCGGCAG GCGGAAAAAA CGTATCCGGT 
TTGACGTTGG ACAAGGTTCA TCGAGGTGTG TTGCTGAGCG AGGGCAACAT GGTCAAGTTG 
GTACTCTTTA TTATCGTGCC CATATGCTGC ATTTTGATGC TGTGCTCCCT GACGTTCTGC 
AGACGAAATC GTTCGGAGGT TCAGGCGCTG CAAATGGACG CTAAGGACGC GAAGGCCAGT 
GAATTTCATC TCTCCCTGAT GGACAGCAGT GGCCTGCTGG TCACCCTCTC GGCCAACGAG 
AGTCTGGAAG TAATGGACGA GCTGGAGGTG GAGCCACACT CGGTGCTCCT TCAGGATGTC 
CTCGGCGAAG GAGCCTTTGG CTTGGTGCGA CGTGGAGTTT ACAAGAAACG CCAAGTGGCC 
GTCAAGTTGC TGAAAGATGA ACCAAACGAC GAGGACGTAT ATGCGTTCAA GTGCGAAATT 
CAGATGCTCA AGGCCGTGGG CAAGCATCCA AATATTGTGG GTATCGTGGG ATACTCCACT 
CGTTTTAGCA ACCAGATGAT GTTGCTAATT GAATACTGCA GCCTTGGAAG CCTGCAGAAC 
TTTTTACGTG AGGAGTGGAA GTTCAGGCAG GAGCAAAATG CAATTGGACT TAAGAAGAAC 
CTTGAACAGA ACGTGGACAA CCGACGGTTT AACCGACTCC CTAGAAATTC CATCCATGAT 
CGCATAGAGG ATATCAACAA CTCGATGCTG TCCACTGTGG AAGAGGAGAG TGAATCGGAT 
CAGACACACT CAAGTCGATG TGAGACCTAC ACCCTCACTC GAATAACCAA TGCAGCCGAC 
AACAAGGGCT ATGGCCTGGA GGACATTGAA AACATCGGTG GGAGTTACAT TCCCAAAACC 
GCTGAAGCTC CAAAGGATCG GCCAAAACGG AAGCTGAAGC CGCAGCCCAA GAAAGACTCG 
AAGCAGGATT TCAAATCGGA CAACAAGAAG CGAATCTTTG AGAACAAGGA ATACTTTGAT 
TGCCTCGACT CATCGGATAC CAAGCCCCGA ATACCACTGA AATATGCAGA TTTGCTAGAC 
ATCGCCCAAC AGGTGGCGGT GGGAATGGAA TTTCTGGCCC AAAACAAAGT AGTGCATAGG 
GATCTGGCTG CCCGGAATGT TCTAATCTCC GTAGATCGCA GCATCAAGAT AGCAGATTTT 
GGGCTGAGTC GAGATGTGTA TCATGAGAAC GTGTACCGAA AGTCCGGAGG AAGTGGCAAG 
CTGCCCATCA AGTGGCTCGC GCTGGAGTCC CTCACCCACC AGGTGTACAC CAGTCAGAGC 
GATGTTTGGT CCTTTGGTGT GCTGCTCTAT GAGATCACCA CTCTCGGTGG AATGCCATAT 
CCGTCGGTGT CTCCCAGTGA TCTCTTGCAG CTACTGCGAC AAGGTCATCG GATGAAGCGA 
CCGGAGGGAT GTACGCAAGA AATGTTTTCC CTGATGGAAA GCTGCTGGAG CTCCGTGCCA 
TCACACAGGC CAACATTTTC CGCCCTTAAA CACAGACTTG GTGGCATGAT TTTGGCCACT 
AACGATGTTC CAGAAAGGCT GAAACAACTG CAAGCTGCAA CCGAGTCAAA ATTAAAGTCA 
TGTGACGGTC TAAACAGTAA AGTGGAGCAA GTGCCATGCG AGGAAGAGCT ATACCTAGAA 
CCTTTGAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tor ( NM_001103742.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097212.2 Drosophila melanogaster torso (tor), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa21699
Clone ID Related Accession (Same CDS sequence)	NM_001103742.2
Accession Version	NM_001103742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	torso, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103742.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTATTT TCTACGCGAA GTACGCATTT ATCTTCTGGT TTTTCGTGGG AAGCAATCAA 
GGTGAAATGT TGCTAATGGA CAAAATCTCT CACGATAAGA CGCTTCTCAA CGTCACCGCT 
TGCACCCAGA ATTGTCTGGA AAAGGGCCAG ATGGATTTCC GAAGCTGTTT AAAGGACTGC 
AGGATTAATG GAACATTTCC CGGGGCTCTG CGCAAGGTGC AGGAAAACTA CCAGATGAAC 
ATGATCTGCC GCACGGAGTC GGAAATCGTT TTCCAAATAG ATTGGGTGCA GCACAGCAGG 
GGAACCGAGC CGGCTCCAAA TGCCACCTAC ATAATCCGGG TGGATGCTGT CAAGGACGAC 
AACAAAGAAA CTGCGCTTTA CCTGTCTGAT GACAACTTTC TCATCCTGCC GGGATTGGAG 
TCCAACTCTA CCCACAACAT CACCGCCCTG GCGATGCACG GAGATGGCAG CTACTCCTTG 
ATAGCAAAGG ACCAGACCTT CGCCACCCTC ATCCGAGGCT ATCAGCCCAG CAAAATGGGA 
GCGGTGAATC TGCTGCGGTT TGTCCCCCAA CCAGACGACC TGCATCACAT TGCTGCCGAA 
ATCGAGTGGA AGCCATCGGC GGAGAGCAAC TGCTATTTCG ACATGGTGTC GTATTCAACC 
AACAGCGTGA ATATGGACGA GCCACTGGAG GTGCAGTTCC GGGATCGCAA AAAGCTGTAC 
AGGCACACGG TGGACAACTT GGAGTTTGAC AAACAGTATC ACGTTGGCGT AAGAACGGTG 
AACATAATGA ATCGACTGGA GAGCGATCTG CAGTGGCTGC CAATCGCTGT TCCAAGCTGC 
TTGGATTGGT ATCCCTATAA CTACACACTC TGCCCACCCC ATAAGCCAGA GAATCTTACT 
GTGACCCAGA AGCAGTATCT GCCAAATATT TTGGCCCTGA ACATCACCTG GGCGCGTCCC 
AGATACCTGC CGGATAACTA TACACTTCAC ATCTTTGATC TATTCAAAGG AGGTACGGAG 
CTAAACTATA CACTTGACCA AAACAGGAGC CACTTCTATG TACCCAAGAT CACGGTACTG 
GGTTCCCATT TCGAAGTACA TTTGGTGGCC CAGTCGGCAG GCGGAAAAAA CGTATCCGGT 
TTGACGTTGG ACAAGGTTCA TCGAGAGGGC AACATGGTCA AGTTGGTACT CTTTATTATC 
GTGCCCATAT GCTGCATTTT GATGCTGTGC TCCCTGACGT TCTGCAGACG AAATCGTTCG 
GAGGTTCAGG CGCTGCAAAT GGACGCTAAG GACGCGAAGG CCAGTGAATT TCATCTCTCC 
CTGATGGACA GCAGTGGCCT GCTGGTCACC CTCTCGGCCA ACGAGAGTCT GGAAGTAATG 
GACGAGCTGG AGGTGGAGCC ACACTCGGTG CTCCTTCAGG ATGTCCTCGG CGAAGGAGCC 
TTTGGCTTGG TGCGACGTGG AGTTTACAAG AAACGCCAAG TGGCCGTCAA GTTGCTGAAA 
GATGAACCAA ACGACGAGGA CGTATATGCG TTCAAGTGCG AAATTCAGAT GCTCAAGGCC 
GTGGGCAAGC ATCCAAATAT TGTGGGTATC GTGGGATACT CCACTCGTTT TAGCAACCAG 
ATGATGTTGC TAATTGAATA CTGCAGCCTT GGAAGCCTGC AGAACTTTTT ACGTGAGGAG 
TGGAAGTTCA GGCAGGAGCA AAATGCAATT GGACTTAAGA AGAACCTTGA ACAGAACGTG 
GACAACCGAC GGTTTAACCG ACTCCCTAGA AATTCCATCC ATGATCGCAT AGAGGATATC 
AACAACTCGA TGCTGTCCAC TGTGGAAGAG GAGAGTGAAT CGGATCAGAC ACACTCAAGT 
CGATGTGAGA CCTACACCCT CACTCGAATA ACCAATGCAG CCGACAACAA GGGCTATGGC 
CTGGAGGACA TTGAAAACAT CGGTGGGAGT TACATTCCCA AAACCGCTGA AGCTCCAAAG 
GATCGGCCAA AACGGAAGCT GAAGCCGCAG CCCAAGAAAG ACTCGAAGCA GGATTTCAAA 
TCGGACAACA AGAAGCGAAT CTTTGAGAAC AAGGAATACT TTGATTGCCT CGACTCATCG 
GATACCAAGC CCCGAATACC ACTGAAATAT GCAGATTTGC TAGACATCGC CCAACAGGTG 
GCGGTGGGAA TGGAATTTCT GGCCCAAAAC AAAGTAGTGC ATAGGGATCT GGCTGCCCGG 
AATGTTCTAA TCTCCGTAGA TCGCAGCATC AAGATAGCAG ATTTTGGGCT GAGTCGAGAT 
GTGTATCATG AGAACGTGTA CCGAAAGTCC GGAGGAAGTG GCAAGCTGCC CATCAAGTGG 
CTCGCGCTGG AGTCCCTCAC CCACCAGGTG TACACCAGTC AGAGCGATGT TTGGTCCTTT 
GGTGTGCTGC TCTATGAGAT CACCACTCTC GGTGGAATGC CATATCCGTC GGTGTCTCCC 
AGTGATCTCT TGCAGCTACT GCGACAAGGT CATCGGATGA AGCGACCGGA GGGATGTACG 
CAAGAAATGT TTTCCCTGAT GGAAAGCTGC TGGAGCTCCG TGCCATCACA CAGGCCAACA 
TTTTCCGCCC TTAAACACAG ACTTGGTGGC ATGATTTTGG CCACTAACGA TGTTCCAGAA 
AGGCTGAAAC AACTGCAAGC TGCAACCGAG TCAAAATTAA AGTCATGTGA CGGTCTAAAC 
AGTAAAGTGG AGCAAGTGCC ATGCGAGGAA GAGCTATACC TAGAACCTTT GAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097212.2
CDS	240..2996
Translation	MLIFYAKYAFIFWFFVGSNQGEMLLMDKISHDKTLLNVTACTQNCLEKGQMDFRSCLKDCRINGTFPGALRKVQENYQMNMICRTESEIVFQIDWVQHSRGTEPAPNATYIIRVDAVKDDNKETALYLSDDNFLILPGLESNSTHNITALAMHGDGSYSLIAKDQTFATLIRGYQPSKMGAVNLLRFVPQPDDLHHIAAEIEWKPSAESNCYFDMVSYSTNSVNMDEPLEVQFRDRKKLYRHTVDNLEFDKQYHVGVRTVNIMNRLESDLQWLPIAVPSCLDWYPYNYTLCPPHKPENLTVTQKQYLPNILALNITWARPRYLPDNYTLHIFDLFKGGTELNYTLDQNRSHFYVPKITVLGSHFEVHLVAQSAGGKNVSGLTLDKVHREGNMVKLVLFIIVPICCILMLCSLTFCRRNRSEVQALQMDAKDAKASEFHLSLMDSSGLLVTLSANESLEVMDELEVEPHSVLLQDVLGEGAFGLVRRGVYKKRQVAVKLLKDEPNDEDVYAFKCEIQMLKAVGKHPNIVGIVGYSTRFSNQMMLLIEYCSLGSLQNFLREEWKFRQEQNAIGLKKNLEQNVDNRRFNRLPRNSIHDRIEDINNSMLSTVEEESESDQTHSSRCETYTLTRITNAADNKGYGLEDIENIGGSYIPKTAEAPKDRPKRKLKPQPKKDSKQDFKSDNKKRIFENKEYFDCLDSSDTKPRIPLKYADLLDIAQQVAVGMEFLAQNKVVHRDLAARNVLISVDRSIKIADFGLSRDVYHENVYRKSGGSGKLPIKWLALESLTHQVYTSQSDVWSFGVLLYEITTLGGMPYPSVSPSDLLQLLRQGHRMKRPEGCTQEMFSLMESCWSSVPSHRPTFSALKHRLGGMILATNDVPERLKQLQAATESKLKSCDGLNSKVEQVPCEEELYLEPLN

Target ORF information:

RefSeq Version	NM_001103742.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster torso (tor), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103742.2

ORF Insert Sequence:

ATGCTTATTT TCTACGCGAA GTACGCATTT ATCTTCTGGT TTTTCGTGGG AAGCAATCAA 
GGTGAAATGT TGCTAATGGA CAAAATCTCT CACGATAAGA CGCTTCTCAA CGTCACCGCT 
TGCACCCAGA ATTGTCTGGA AAAGGGCCAG ATGGATTTCC GAAGCTGTTT AAAGGACTGC 
AGGATTAATG GAACATTTCC CGGGGCTCTG CGCAAGGTGC AGGAAAACTA CCAGATGAAC 
ATGATCTGCC GCACGGAGTC GGAAATCGTT TTCCAAATAG ATTGGGTGCA GCACAGCAGG 
GGAACCGAGC CGGCTCCAAA TGCCACCTAC ATAATCCGGG TGGATGCTGT CAAGGACGAC 
AACAAAGAAA CTGCGCTTTA CCTGTCTGAT GACAACTTTC TCATCCTGCC GGGATTGGAG 
TCCAACTCTA CCCACAACAT CACCGCCCTG GCGATGCACG GAGATGGCAG CTACTCCTTG 
ATAGCAAAGG ACCAGACCTT CGCCACCCTC ATCCGAGGCT ATCAGCCCAG CAAAATGGGA 
GCGGTGAATC TGCTGCGGTT TGTCCCCCAA CCAGACGACC TGCATCACAT TGCTGCCGAA 
ATCGAGTGGA AGCCATCGGC GGAGAGCAAC TGCTATTTCG ACATGGTGTC GTATTCAACC 
AACAGCGTGA ATATGGACGA GCCACTGGAG GTGCAGTTCC GGGATCGCAA AAAGCTGTAC 
AGGCACACGG TGGACAACTT GGAGTTTGAC AAACAGTATC ACGTTGGCGT AAGAACGGTG 
AACATAATGA ATCGACTGGA GAGCGATCTG CAGTGGCTGC CAATCGCTGT TCCAAGCTGC 
TTGGATTGGT ATCCCTATAA CTACACACTC TGCCCACCCC ATAAGCCAGA GAATCTTACT 
GTGACCCAGA AGCAGTATCT GCCAAATATT TTGGCCCTGA ACATCACCTG GGCGCGTCCC 
AGATACCTGC CGGATAACTA TACACTTCAC ATCTTTGATC TATTCAAAGG AGGTACGGAG 
CTAAACTATA CACTTGACCA AAACAGGAGC CACTTCTATG TACCCAAGAT CACGGTACTG 
GGTTCCCATT TCGAAGTACA TTTGGTGGCC CAGTCGGCAG GCGGAAAAAA CGTATCCGGT 
TTGACGTTGG ACAAGGTTCA TCGAGAGGGC AACATGGTCA AGTTGGTACT CTTTATTATC 
GTGCCCATAT GCTGCATTTT GATGCTGTGC TCCCTGACGT TCTGCAGACG AAATCGTTCG 
GAGGTTCAGG CGCTGCAAAT GGACGCTAAG GACGCGAAGG CCAGTGAATT TCATCTCTCC 
CTGATGGACA GCAGTGGCCT GCTGGTCACC CTCTCGGCCA ACGAGAGTCT GGAAGTAATG 
GACGAGCTGG AGGTGGAGCC ACACTCGGTG CTCCTTCAGG ATGTCCTCGG CGAAGGAGCC 
TTTGGCTTGG TGCGACGTGG AGTTTACAAG AAACGCCAAG TGGCCGTCAA GTTGCTGAAA 
GATGAACCAA ACGACGAGGA CGTATATGCG TTCAAGTGCG AAATTCAGAT GCTCAAGGCC 
GTGGGCAAGC ATCCAAATAT TGTGGGTATC GTGGGATACT CCACTCGTTT TAGCAACCAG 
ATGATGTTGC TAATTGAATA CTGCAGCCTT GGAAGCCTGC AGAACTTTTT ACGTGAGGAG 
TGGAAGTTCA GGCAGGAGCA AAATGCAATT GGACTTAAGA AGAACCTTGA ACAGAACGTG 
GACAACCGAC GGTTTAACCG ACTCCCTAGA AATTCCATCC ATGATCGCAT AGAGGATATC 
AACAACTCGA TGCTGTCCAC TGTGGAAGAG GAGAGTGAAT CGGATCAGAC ACACTCAAGT 
CGATGTGAGA CCTACACCCT CACTCGAATA ACCAATGCAG CCGACAACAA GGGCTATGGC 
CTGGAGGACA TTGAAAACAT CGGTGGGAGT TACATTCCCA AAACCGCTGA AGCTCCAAAG 
GATCGGCCAA AACGGAAGCT GAAGCCGCAG CCCAAGAAAG ACTCGAAGCA GGATTTCAAA 
TCGGACAACA AGAAGCGAAT CTTTGAGAAC AAGGAATACT TTGATTGCCT CGACTCATCG 
GATACCAAGC CCCGAATACC ACTGAAATAT GCAGATTTGC TAGACATCGC CCAACAGGTG 
GCGGTGGGAA TGGAATTTCT GGCCCAAAAC AAAGTAGTGC ATAGGGATCT GGCTGCCCGG 
AATGTTCTAA TCTCCGTAGA TCGCAGCATC AAGATAGCAG ATTTTGGGCT GAGTCGAGAT 
GTGTATCATG AGAACGTGTA CCGAAAGTCC GGAGGAAGTG GCAAGCTGCC CATCAAGTGG 
CTCGCGCTGG AGTCCCTCAC CCACCAGGTG TACACCAGTC AGAGCGATGT TTGGTCCTTT 
GGTGTGCTGC TCTATGAGAT CACCACTCTC GGTGGAATGC CATATCCGTC GGTGTCTCCC 
AGTGATCTCT TGCAGCTACT GCGACAAGGT CATCGGATGA AGCGACCGGA GGGATGTACG 
CAAGAAATGT TTTCCCTGAT GGAAAGCTGC TGGAGCTCCG TGCCATCACA CAGGCCAACA 
TTTTCCGCCC TTAAACACAG ACTTGGTGGC ATGATTTTGG CCACTAACGA TGTTCCAGAA 
AGGCTGAAAC AACTGCAAGC TGCAACCGAG TCAAAATTAA AGTCATGTGA CGGTCTAAAC 
AGTAAAGTGG AGCAAGTGCC ATGCGAGGAA GAGCTATACC TAGAACCTTT GAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tor cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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