chk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	chk
Entrez Gene ID	35578
Full Name	CG3409 gene product from transcript CG3409-RB
Synonyms	Dmel\CG3409
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42C5-42C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_136386.4	NP_610230.3	chaski, isoform A
NM_001273804.1	NP_001260733.1	chaski, isoform C
NM_001169592.2	NP_001163063.1	chaski, isoform B

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MCH5	NP_014951.4
Magnaporthe oryzae (rice blast fungus)	MGG_10881	XP_003719365.1
Magnaporthe oryzae (rice blast fungus)	MGG_17983	XP_003721035.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005394	XP_315403.4
Kluyveromyces lactis	KLLA0F08525g	XP_455467.1
Drosophila melanogaster (fruit fly)	CG3409	NP_610230.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09049	NM_136386.4 Latest version!	Drosophila melanogaster chaski (chk), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09049	NM_001273804.1 Latest version!	Drosophila melanogaster chaski (chk), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09049	NM_001169592.2 Latest version!	Drosophila melanogaster chaski (chk), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chk ( NM_136386.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610230.3 Drosophila melanogaster chaski (chk), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09049
Clone ID Related Accession (Same CDS sequence)	NM_136386.4 , NM_001273804.1 , NM_001169592.2
Accession Version	NM_136386.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chaski, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136386.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAGC TAGCGACGGA TCCAAACGCC TCCAACAACA ACAGCTGCGA GGCGGTTAAC 
AACAACCACA ATGGCCAGAA CCAGAGCCAA AACGGAGGCG CCTCCAATTA CCAGGCCCTG 
CCTCTTACGC CTGCTCCGGC CAACACGCCA CTCCACAAGG CCATCAAGCA CGACCTCTTC 
CCGGAGGTCA CCTTCTGCAA CCTCTCCGTA GAGGAGCTCG CAGATGGGGC TGGACACAGC 
CGTGTGGTAA GGAGCAGTGT CATCGAACTG GAGGACGGCA CCATGACGTG CCTGATGAAC 
GGAAATGGGC AGGTGAAGCG TCGCAAACGC CTGATCTCGA ACGAGTCCGG CGACTCGATC 
GACTCCAGTT CCACGGAGAA GAAAACGCCC AAGATGCCCG ACGGTGGATA TGGGTGGGTG 
GTGGTGTTCG CCTCTCTGGT CGTGTCCCTT ATAGCCGACG GACTGAGCTT CTCATTTGGC 
CTAATAAACG TGGAACTGCT GGAATACTTC GGAGAGTCCA CTTCGAAGAC CGCCTGGATA 
TCCTCGCTAT TCTTCTCCGT GCCCCTGTTG ATGGGACCCA TCTGGTCCAA TCTGGTGGAC 
AAGTACGGCT GCCGAAAGAT GACCATCCTC GGCGGAGTGG TCTCTGCCTT TGGGTTTGCT 
CTGTCTTCGT TTTGCAACTC GATCGAGATG CTCATGGTGA CCTTTGGTAT CATTTCGGGA 
CTGGGCCTAG GCATCGGGTA TGTGACCGCG GTGGTGTCCA TTGCATTCTG GTTTGACAAG 
AAGCGCACCT TTGCGACCGG CATCGGAGCA TCGGGCACCG GCATCGGGAC CTTTGTCTAC 
GCCCGCCTTA CTTCCTATCT AATCGAATCG TACGGCTGGC GCGGAGCCAC TCTAATCCTT 
GGCGGCACCA TGCTCAACGC ATGCGTTTGC GGCGCCCTCA TGCGCGATCC CGACTGGCTG 
ATCGAGGAAA ACCGTCTAGA GAGTCGATCC CAAAGCGTCA CCACCTTCTC GAACTCCAGT 
GTCTGCCTGG AGGAAATAAA AAAGCTGCTG GACACCGGCA TCACAAAGGA GGCTGTGCTA 
GACTCGTTGG TGACCAAGAA CAACACGGAG GCCAACCAGC AGATTGACGA TCCGTTGGAC 
TCCGCTCTCA AGCGCTACCG CAGCGAGATC TTCCTGCCCA CCTTCCTGAG CACTCAGGAG 
CTTGACAGCA TCTGCGAGGT TAAGAGCCTG AGCCGACGCT CCCTACGCCA CAAGGAGGGT 
GAGGAGGCGC CGTCACGAGA GAACCTGCTG TCCATGTCCT CAGGAGCAGG TGCCTATCCC 
CCATCGACGG CCGTTATAGG CTCGCCAGAT GATACCCTAA TGGGCGGAAT AGCTCACGAG 
GCGGCAGAGG AAGCAAAGAA GGCCTATCTG GCCTCCATCG AGACGCTGTC TCCATCGGAA 
AAGCGCTCCA CTGGTAGGAC TCCCAATGGC TCGCTCCGCT CCTCGGACGA AGGCTACCTT 
ACGCAGAAGC ACGCCAGCTC CCGGTACTCG CTTAACGAAA ACATCTTCAT GGCCAAGCAT 
ACAACACCAT CAATCTCCAA CCTAAAGGTG AATGGCCTGC GACACAATTC GGTGGACATC 
TTGAGCGAGG ACATGCACTG CTACTACTCG AAGGACGAGA CCTTCGCATT GCTGGAGCCG 
AGCAGGCGCA TCAGGCCCAC CGTCATCGCC ATTCCGGAAC AGGAGCAGTC GGTGAACAGC 
GAGCTGGCCA CCCGTCGCGC ACGCCTGGAC AGCATCACGG GAATCCGCCG GCTGTCGCGC 
TCCAAGAAGC CCAGCCACCA CCGATCGAAC CTCCGCCGCA ACATCTCGAT CCGGAACTCC 
AACTTCCTTA AGGATATGCG CATTCACCGC AACTCGATCC ACTACCGCGG GGCCATGCTG 
AACACGCACC GCTACCGGCT GCGCGCCTCC TCCTGTCCCA ACATCTACCG CAACTCGATG 
ACCACCATCG CCAAAGAGGA GGAAGATACC TGGTATGACA ACTTTGTGGA CACCATGAAG 
TCCATCTTTG ACTTTTCACT CTTTCTGGAC ATGAAGTTCG CCCTCTTCAA TCTCTCTACA 
TTGTTTTTGT TCATTTGGTT CATTATCCCC TATCTGTACC TTCCCGATTA CATGAAGCAA 
TACAAGTACG ACGTGAGCCA GAGTGCCGAG TTGATTTCGG ATGTCGGCAT TGCCCAAACG 
GTGGGCATGA TAGGACTTGG CTATCTGGGG GATCTGTCCT GGATGAACAT CAATATATGC 
TACTCGCTGT GCATGCTGGT GTGCGGAGCG TCTGTGTTCT TCATGCCCCT GCTGATTACC 
AACTACATGG GCCTGATGGT CATGTGCGTT ATTTTCGGAT TTACGTTCGC CAGCTCCTTC 
TCGTTTACGC CCAGCATTCT GGTCAGTATT GTGGATCTGG ACGATTTCAC GTGTGCCTAC 
GGTCTGGTGC TGCTGGTTCA GGGAGTTGGA ATGATCGCAG GACCCCCTAT TGCAGGCGTC 
ATATACGAGT ATACGGGCAG ATGGGATGAC ACCTTCTATT ATGCTGGAAT ATTTATCGCA 
CTCTCTGGTG TCTGTTCGTA TCTGATCGAG TTCTGTGAAA AGAAAGCACC TAAGGAGAGT 
GATAGTGATG TCTTAGAGAC TAAAAAAGCT CAACTTTTAC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610230.3
CDS	1947..4631
Translation	MAKLATDPNASNNNSCEAVNNNHNGQNQSQNGGASNYQALPLTPAPANTPLHKAIKHDLFPEVTFCNLSVEELADGAGHSRVVRSSVIELEDGTMTCLMNGNGQVKRRKRLISNESGDSIDSSSTEKKTPKMPDGGYGWVVVFASLVVSLIADGLSFSFGLINVELLEYFGESTSKTAWISSLFFSVPLLMGPIWSNLVDKYGCRKMTILGGVVSAFGFALSSFCNSIEMLMVTFGIISGLGLGIGYVTAVVSIAFWFDKKRTFATGIGASGTGIGTFVYARLTSYLIESYGWRGATLILGGTMLNACVCGALMRDPDWLIEENRLESRSQSVTTFSNSSVCLEEIKKLLDTGITKEAVLDSLVTKNNTEANQQIDDPLDSALKRYRSEIFLPTFLSTQELDSICEVKSLSRRSLRHKEGEEAPSRENLLSMSSGAGAYPPSTAVIGSPDDTLMGGIAHEAAEEAKKAYLASIETLSPSEKRSTGRTPNGSLRSSDEGYLTQKHASSRYSLNENIFMAKHTTPSISNLKVNGLRHNSVDILSEDMHCYYSKDETFALLEPSRRIRPTVIAIPEQEQSVNSELATRRARLDSITGIRRLSRSKKPSHHRSNLRRNISIRNSNFLKDMRIHRNSIHYRGAMLNTHRYRLRASSCPNIYRNSMTTIAKEEEDTWYDNFVDTMKSIFDFSLFLDMKFALFNLSTLFLFIWFIIPYLYLPDYMKQYKYDVSQSAELISDVGIAQTVGMIGLGYLGDLSWMNINICYSLCMLVCGASVFFMPLLITNYMGLMVMCVIFGFTFASSFSFTPSILVSIVDLDDFTCAYGLVLLVQGVGMIAGPPIAGVIYEYTGRWDDTFYYAGIFIALSGVCSYLIEFCEKKAPKESDSDVLETKKAQLLH

Target ORF information:

RefSeq Version	NM_136386.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chaski (chk), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136386.4

ORF Insert Sequence:

ATGGCCAAGC TAGCGACGGA TCCAAACGCC TCCAACAACA ACAGCTGCGA GGCGGTTAAC 
AACAACCACA ATGGCCAGAA CCAGAGCCAA AACGGAGGCG CCTCCAATTA CCAGGCCCTG 
CCTCTTACGC CTGCTCCGGC CAACACGCCA CTCCACAAGG CCATCAAGCA CGACCTCTTC 
CCGGAGGTCA CCTTCTGCAA CCTCTCCGTA GAGGAGCTCG CAGATGGGGC TGGACACAGC 
CGTGTGGTAA GGAGCAGTGT CATCGAACTG GAGGACGGCA CCATGACGTG CCTGATGAAC 
GGAAATGGGC AGGTGAAGCG TCGCAAACGC CTGATCTCGA ACGAGTCCGG CGACTCGATC 
GACTCCAGTT CCACGGAGAA GAAAACGCCC AAGATGCCCG ACGGTGGATA TGGGTGGGTG 
GTGGTGTTCG CCTCTCTGGT CGTGTCCCTT ATAGCCGACG GACTGAGCTT CTCATTTGGC 
CTAATAAACG TGGAACTGCT GGAATACTTC GGAGAGTCCA CTTCGAAGAC CGCCTGGATA 
TCCTCGCTAT TCTTCTCCGT GCCCCTGTTG ATGGGACCCA TCTGGTCCAA TCTGGTGGAC 
AAGTACGGCT GCCGAAAGAT GACCATCCTC GGCGGAGTGG TCTCTGCCTT TGGGTTTGCT 
CTGTCTTCGT TTTGCAACTC GATCGAGATG CTCATGGTGA CCTTTGGTAT CATTTCGGGA 
CTGGGCCTAG GCATCGGGTA TGTGACCGCG GTGGTGTCCA TTGCATTCTG GTTTGACAAG 
AAGCGCACCT TTGCGACCGG CATCGGAGCA TCGGGCACCG GCATCGGGAC CTTTGTCTAC 
GCCCGCCTTA CTTCCTATCT AATCGAATCG TACGGCTGGC GCGGAGCCAC TCTAATCCTT 
GGCGGCACCA TGCTCAACGC ATGCGTTTGC GGCGCCCTCA TGCGCGATCC CGACTGGCTG 
ATCGAGGAAA ACCGTCTAGA GAGTCGATCC CAAAGCGTCA CCACCTTCTC GAACTCCAGT 
GTCTGCCTGG AGGAAATAAA AAAGCTGCTG GACACCGGCA TCACAAAGGA GGCTGTGCTA 
GACTCGTTGG TGACCAAGAA CAACACGGAG GCCAACCAGC AGATTGACGA TCCGTTGGAC 
TCCGCTCTCA AGCGCTACCG CAGCGAGATC TTCCTGCCCA CCTTCCTGAG CACTCAGGAG 
CTTGACAGCA TCTGCGAGGT TAAGAGCCTG AGCCGACGCT CCCTACGCCA CAAGGAGGGT 
GAGGAGGCGC CGTCACGAGA GAACCTGCTG TCCATGTCCT CAGGAGCAGG TGCCTATCCC 
CCATCGACGG CCGTTATAGG CTCGCCAGAT GATACCCTAA TGGGCGGAAT AGCTCACGAG 
GCGGCAGAGG AAGCAAAGAA GGCCTATCTG GCCTCCATCG AGACGCTGTC TCCATCGGAA 
AAGCGCTCCA CTGGTAGGAC TCCCAATGGC TCGCTCCGCT CCTCGGACGA AGGCTACCTT 
ACGCAGAAGC ACGCCAGCTC CCGGTACTCG CTTAACGAAA ACATCTTCAT GGCCAAGCAT 
ACAACACCAT CAATCTCCAA CCTAAAGGTG AATGGCCTGC GACACAATTC GGTGGACATC 
TTGAGCGAGG ACATGCACTG CTACTACTCG AAGGACGAGA CCTTCGCATT GCTGGAGCCG 
AGCAGGCGCA TCAGGCCCAC CGTCATCGCC ATTCCGGAAC AGGAGCAGTC GGTGAACAGC 
GAGCTGGCCA CCCGTCGCGC ACGCCTGGAC AGCATCACGG GAATCCGCCG GCTGTCGCGC 
TCCAAGAAGC CCAGCCACCA CCGATCGAAC CTCCGCCGCA ACATCTCGAT CCGGAACTCC 
AACTTCCTTA AGGATATGCG CATTCACCGC AACTCGATCC ACTACCGCGG GGCCATGCTG 
AACACGCACC GCTACCGGCT GCGCGCCTCC TCCTGTCCCA ACATCTACCG CAACTCGATG 
ACCACCATCG CCAAAGAGGA GGAAGATACC TGGTATGACA ACTTTGTGGA CACCATGAAG 
TCCATCTTTG ACTTTTCACT CTTTCTGGAC ATGAAGTTCG CCCTCTTCAA TCTCTCTACA 
TTGTTTTTGT TCATTTGGTT CATTATCCCC TATCTGTACC TTCCCGATTA CATGAAGCAA 
TACAAGTACG ACGTGAGCCA GAGTGCCGAG TTGATTTCGG ATGTCGGCAT TGCCCAAACG 
GTGGGCATGA TAGGACTTGG CTATCTGGGG GATCTGTCCT GGATGAACAT CAATATATGC 
TACTCGCTGT GCATGCTGGT GTGCGGAGCG TCTGTGTTCT TCATGCCCCT GCTGATTACC 
AACTACATGG GCCTGATGGT CATGTGCGTT ATTTTCGGAT TTACGTTCGC CAGCTCCTTC 
TCGTTTACGC CCAGCATTCT GGTCAGTATT GTGGATCTGG ACGATTTCAC GTGTGCCTAC 
GGTCTGGTGC TGCTGGTTCA GGGAGTTGGA ATGATCGCAG GACCCCCTAT TGCAGGCGTC 
ATATACGAGT ATACGGGCAG ATGGGATGAC ACCTTCTATT ATGCTGGAAT ATTTATCGCA 
CTCTCTGGTG TCTGTTCGTA TCTGATCGAG TTCTGTGAAA AGAAAGCACC TAAGGAGAGT 
GATAGTGATG TCTTAGAGAC TAAAAAAGCT CAACTTTTAC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chk ( NM_001273804.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260733.1 Drosophila melanogaster chaski (chk), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa09049
Clone ID Related Accession (Same CDS sequence)	NM_136386.4 , NM_001273804.1 , NM_001169592.2
Accession Version	NM_001273804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chaski, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAGC TAGCGACGGA TCCAAACGCC TCCAACAACA ACAGCTGCGA GGCGGTTAAC 
AACAACCACA ATGGCCAGAA CCAGAGCCAA AACGGAGGCG CCTCCAATTA CCAGGCCCTG 
CCTCTTACGC CTGCTCCGGC CAACACGCCA CTCCACAAGG CCATCAAGCA CGACCTCTTC 
CCGGAGGTCA CCTTCTGCAA CCTCTCCGTA GAGGAGCTCG CAGATGGGGC TGGACACAGC 
CGTGTGGTAA GGAGCAGTGT CATCGAACTG GAGGACGGCA CCATGACGTG CCTGATGAAC 
GGAAATGGGC AGGTGAAGCG TCGCAAACGC CTGATCTCGA ACGAGTCCGG CGACTCGATC 
GACTCCAGTT CCACGGAGAA GAAAACGCCC AAGATGCCCG ACGGTGGATA TGGGTGGGTG 
GTGGTGTTCG CCTCTCTGGT CGTGTCCCTT ATAGCCGACG GACTGAGCTT CTCATTTGGC 
CTAATAAACG TGGAACTGCT GGAATACTTC GGAGAGTCCA CTTCGAAGAC CGCCTGGATA 
TCCTCGCTAT TCTTCTCCGT GCCCCTGTTG ATGGGACCCA TCTGGTCCAA TCTGGTGGAC 
AAGTACGGCT GCCGAAAGAT GACCATCCTC GGCGGAGTGG TCTCTGCCTT TGGGTTTGCT 
CTGTCTTCGT TTTGCAACTC GATCGAGATG CTCATGGTGA CCTTTGGTAT CATTTCGGGA 
CTGGGCCTAG GCATCGGGTA TGTGACCGCG GTGGTGTCCA TTGCATTCTG GTTTGACAAG 
AAGCGCACCT TTGCGACCGG CATCGGAGCA TCGGGCACCG GCATCGGGAC CTTTGTCTAC 
GCCCGCCTTA CTTCCTATCT AATCGAATCG TACGGCTGGC GCGGAGCCAC TCTAATCCTT 
GGCGGCACCA TGCTCAACGC ATGCGTTTGC GGCGCCCTCA TGCGCGATCC CGACTGGCTG 
ATCGAGGAAA ACCGTCTAGA GAGTCGATCC CAAAGCGTCA CCACCTTCTC GAACTCCAGT 
GTCTGCCTGG AGGAAATAAA AAAGCTGCTG GACACCGGCA TCACAAAGGA GGCTGTGCTA 
GACTCGTTGG TGACCAAGAA CAACACGGAG GCCAACCAGC AGATTGACGA TCCGTTGGAC 
TCCGCTCTCA AGCGCTACCG CAGCGAGATC TTCCTGCCCA CCTTCCTGAG CACTCAGGAG 
CTTGACAGCA TCTGCGAGGT TAAGAGCCTG AGCCGACGCT CCCTACGCCA CAAGGAGGGT 
GAGGAGGCGC CGTCACGAGA GAACCTGCTG TCCATGTCCT CAGGAGCAGG TGCCTATCCC 
CCATCGACGG CCGTTATAGG CTCGCCAGAT GATACCCTAA TGGGCGGAAT AGCTCACGAG 
GCGGCAGAGG AAGCAAAGAA GGCCTATCTG GCCTCCATCG AGACGCTGTC TCCATCGGAA 
AAGCGCTCCA CTGGTAGGAC TCCCAATGGC TCGCTCCGCT CCTCGGACGA AGGCTACCTT 
ACGCAGAAGC ACGCCAGCTC CCGGTACTCG CTTAACGAAA ACATCTTCAT GGCCAAGCAT 
ACAACACCAT CAATCTCCAA CCTAAAGGTG AATGGCCTGC GACACAATTC GGTGGACATC 
TTGAGCGAGG ACATGCACTG CTACTACTCG AAGGACGAGA CCTTCGCATT GCTGGAGCCG 
AGCAGGCGCA TCAGGCCCAC CGTCATCGCC ATTCCGGAAC AGGAGCAGTC GGTGAACAGC 
GAGCTGGCCA CCCGTCGCGC ACGCCTGGAC AGCATCACGG GAATCCGCCG GCTGTCGCGC 
TCCAAGAAGC CCAGCCACCA CCGATCGAAC CTCCGCCGCA ACATCTCGAT CCGGAACTCC 
AACTTCCTTA AGGATATGCG CATTCACCGC AACTCGATCC ACTACCGCGG GGCCATGCTG 
AACACGCACC GCTACCGGCT GCGCGCCTCC TCCTGTCCCA ACATCTACCG CAACTCGATG 
ACCACCATCG CCAAAGAGGA GGAAGATACC TGGTATGACA ACTTTGTGGA CACCATGAAG 
TCCATCTTTG ACTTTTCACT CTTTCTGGAC ATGAAGTTCG CCCTCTTCAA TCTCTCTACA 
TTGTTTTTGT TCATTTGGTT CATTATCCCC TATCTGTACC TTCCCGATTA CATGAAGCAA 
TACAAGTACG ACGTGAGCCA GAGTGCCGAG TTGATTTCGG ATGTCGGCAT TGCCCAAACG 
GTGGGCATGA TAGGACTTGG CTATCTGGGG GATCTGTCCT GGATGAACAT CAATATATGC 
TACTCGCTGT GCATGCTGGT GTGCGGAGCG TCTGTGTTCT TCATGCCCCT GCTGATTACC 
AACTACATGG GCCTGATGGT CATGTGCGTT ATTTTCGGAT TTACGTTCGC CAGCTCCTTC 
TCGTTTACGC CCAGCATTCT GGTCAGTATT GTGGATCTGG ACGATTTCAC GTGTGCCTAC 
GGTCTGGTGC TGCTGGTTCA GGGAGTTGGA ATGATCGCAG GACCCCCTAT TGCAGGCGTC 
ATATACGAGT ATACGGGCAG ATGGGATGAC ACCTTCTATT ATGCTGGAAT ATTTATCGCA 
CTCTCTGGTG TCTGTTCGTA TCTGATCGAG TTCTGTGAAA AGAAAGCACC TAAGGAGAGT 
GATAGTGATG TCTTAGAGAC TAAAAAAGCT CAACTTTTAC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260733.1
CDS	1360..4044
Translation	MAKLATDPNASNNNSCEAVNNNHNGQNQSQNGGASNYQALPLTPAPANTPLHKAIKHDLFPEVTFCNLSVEELADGAGHSRVVRSSVIELEDGTMTCLMNGNGQVKRRKRLISNESGDSIDSSSTEKKTPKMPDGGYGWVVVFASLVVSLIADGLSFSFGLINVELLEYFGESTSKTAWISSLFFSVPLLMGPIWSNLVDKYGCRKMTILGGVVSAFGFALSSFCNSIEMLMVTFGIISGLGLGIGYVTAVVSIAFWFDKKRTFATGIGASGTGIGTFVYARLTSYLIESYGWRGATLILGGTMLNACVCGALMRDPDWLIEENRLESRSQSVTTFSNSSVCLEEIKKLLDTGITKEAVLDSLVTKNNTEANQQIDDPLDSALKRYRSEIFLPTFLSTQELDSICEVKSLSRRSLRHKEGEEAPSRENLLSMSSGAGAYPPSTAVIGSPDDTLMGGIAHEAAEEAKKAYLASIETLSPSEKRSTGRTPNGSLRSSDEGYLTQKHASSRYSLNENIFMAKHTTPSISNLKVNGLRHNSVDILSEDMHCYYSKDETFALLEPSRRIRPTVIAIPEQEQSVNSELATRRARLDSITGIRRLSRSKKPSHHRSNLRRNISIRNSNFLKDMRIHRNSIHYRGAMLNTHRYRLRASSCPNIYRNSMTTIAKEEEDTWYDNFVDTMKSIFDFSLFLDMKFALFNLSTLFLFIWFIIPYLYLPDYMKQYKYDVSQSAELISDVGIAQTVGMIGLGYLGDLSWMNINICYSLCMLVCGASVFFMPLLITNYMGLMVMCVIFGFTFASSFSFTPSILVSIVDLDDFTCAYGLVLLVQGVGMIAGPPIAGVIYEYTGRWDDTFYYAGIFIALSGVCSYLIEFCEKKAPKESDSDVLETKKAQLLH

Target ORF information:

RefSeq Version	NM_001273804.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chaski (chk), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273804.1

ORF Insert Sequence:

ATGGCCAAGC TAGCGACGGA TCCAAACGCC TCCAACAACA ACAGCTGCGA GGCGGTTAAC 
AACAACCACA ATGGCCAGAA CCAGAGCCAA AACGGAGGCG CCTCCAATTA CCAGGCCCTG 
CCTCTTACGC CTGCTCCGGC CAACACGCCA CTCCACAAGG CCATCAAGCA CGACCTCTTC 
CCGGAGGTCA CCTTCTGCAA CCTCTCCGTA GAGGAGCTCG CAGATGGGGC TGGACACAGC 
CGTGTGGTAA GGAGCAGTGT CATCGAACTG GAGGACGGCA CCATGACGTG CCTGATGAAC 
GGAAATGGGC AGGTGAAGCG TCGCAAACGC CTGATCTCGA ACGAGTCCGG CGACTCGATC 
GACTCCAGTT CCACGGAGAA GAAAACGCCC AAGATGCCCG ACGGTGGATA TGGGTGGGTG 
GTGGTGTTCG CCTCTCTGGT CGTGTCCCTT ATAGCCGACG GACTGAGCTT CTCATTTGGC 
CTAATAAACG TGGAACTGCT GGAATACTTC GGAGAGTCCA CTTCGAAGAC CGCCTGGATA 
TCCTCGCTAT TCTTCTCCGT GCCCCTGTTG ATGGGACCCA TCTGGTCCAA TCTGGTGGAC 
AAGTACGGCT GCCGAAAGAT GACCATCCTC GGCGGAGTGG TCTCTGCCTT TGGGTTTGCT 
CTGTCTTCGT TTTGCAACTC GATCGAGATG CTCATGGTGA CCTTTGGTAT CATTTCGGGA 
CTGGGCCTAG GCATCGGGTA TGTGACCGCG GTGGTGTCCA TTGCATTCTG GTTTGACAAG 
AAGCGCACCT TTGCGACCGG CATCGGAGCA TCGGGCACCG GCATCGGGAC CTTTGTCTAC 
GCCCGCCTTA CTTCCTATCT AATCGAATCG TACGGCTGGC GCGGAGCCAC TCTAATCCTT 
GGCGGCACCA TGCTCAACGC ATGCGTTTGC GGCGCCCTCA TGCGCGATCC CGACTGGCTG 
ATCGAGGAAA ACCGTCTAGA GAGTCGATCC CAAAGCGTCA CCACCTTCTC GAACTCCAGT 
GTCTGCCTGG AGGAAATAAA AAAGCTGCTG GACACCGGCA TCACAAAGGA GGCTGTGCTA 
GACTCGTTGG TGACCAAGAA CAACACGGAG GCCAACCAGC AGATTGACGA TCCGTTGGAC 
TCCGCTCTCA AGCGCTACCG CAGCGAGATC TTCCTGCCCA CCTTCCTGAG CACTCAGGAG 
CTTGACAGCA TCTGCGAGGT TAAGAGCCTG AGCCGACGCT CCCTACGCCA CAAGGAGGGT 
GAGGAGGCGC CGTCACGAGA GAACCTGCTG TCCATGTCCT CAGGAGCAGG TGCCTATCCC 
CCATCGACGG CCGTTATAGG CTCGCCAGAT GATACCCTAA TGGGCGGAAT AGCTCACGAG 
GCGGCAGAGG AAGCAAAGAA GGCCTATCTG GCCTCCATCG AGACGCTGTC TCCATCGGAA 
AAGCGCTCCA CTGGTAGGAC TCCCAATGGC TCGCTCCGCT CCTCGGACGA AGGCTACCTT 
ACGCAGAAGC ACGCCAGCTC CCGGTACTCG CTTAACGAAA ACATCTTCAT GGCCAAGCAT 
ACAACACCAT CAATCTCCAA CCTAAAGGTG AATGGCCTGC GACACAATTC GGTGGACATC 
TTGAGCGAGG ACATGCACTG CTACTACTCG AAGGACGAGA CCTTCGCATT GCTGGAGCCG 
AGCAGGCGCA TCAGGCCCAC CGTCATCGCC ATTCCGGAAC AGGAGCAGTC GGTGAACAGC 
GAGCTGGCCA CCCGTCGCGC ACGCCTGGAC AGCATCACGG GAATCCGCCG GCTGTCGCGC 
TCCAAGAAGC CCAGCCACCA CCGATCGAAC CTCCGCCGCA ACATCTCGAT CCGGAACTCC 
AACTTCCTTA AGGATATGCG CATTCACCGC AACTCGATCC ACTACCGCGG GGCCATGCTG 
AACACGCACC GCTACCGGCT GCGCGCCTCC TCCTGTCCCA ACATCTACCG CAACTCGATG 
ACCACCATCG CCAAAGAGGA GGAAGATACC TGGTATGACA ACTTTGTGGA CACCATGAAG 
TCCATCTTTG ACTTTTCACT CTTTCTGGAC ATGAAGTTCG CCCTCTTCAA TCTCTCTACA 
TTGTTTTTGT TCATTTGGTT CATTATCCCC TATCTGTACC TTCCCGATTA CATGAAGCAA 
TACAAGTACG ACGTGAGCCA GAGTGCCGAG TTGATTTCGG ATGTCGGCAT TGCCCAAACG 
GTGGGCATGA TAGGACTTGG CTATCTGGGG GATCTGTCCT GGATGAACAT CAATATATGC 
TACTCGCTGT GCATGCTGGT GTGCGGAGCG TCTGTGTTCT TCATGCCCCT GCTGATTACC 
AACTACATGG GCCTGATGGT CATGTGCGTT ATTTTCGGAT TTACGTTCGC CAGCTCCTTC 
TCGTTTACGC CCAGCATTCT GGTCAGTATT GTGGATCTGG ACGATTTCAC GTGTGCCTAC 
GGTCTGGTGC TGCTGGTTCA GGGAGTTGGA ATGATCGCAG GACCCCCTAT TGCAGGCGTC 
ATATACGAGT ATACGGGCAG ATGGGATGAC ACCTTCTATT ATGCTGGAAT ATTTATCGCA 
CTCTCTGGTG TCTGTTCGTA TCTGATCGAG TTCTGTGAAA AGAAAGCACC TAAGGAGAGT 
GATAGTGATG TCTTAGAGAC TAAAAAAGCT CAACTTTTAC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chk ( NM_001169592.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163063.1 Drosophila melanogaster chaski (chk), transcript variant B, mRNA.

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CloneID	OFa09049
Clone ID Related Accession (Same CDS sequence)	NM_136386.4 , NM_001273804.1 , NM_001169592.2
Accession Version	NM_001169592.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chaski, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169592.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAGC TAGCGACGGA TCCAAACGCC TCCAACAACA ACAGCTGCGA GGCGGTTAAC 
AACAACCACA ATGGCCAGAA CCAGAGCCAA AACGGAGGCG CCTCCAATTA CCAGGCCCTG 
CCTCTTACGC CTGCTCCGGC CAACACGCCA CTCCACAAGG CCATCAAGCA CGACCTCTTC 
CCGGAGGTCA CCTTCTGCAA CCTCTCCGTA GAGGAGCTCG CAGATGGGGC TGGACACAGC 
CGTGTGGTAA GGAGCAGTGT CATCGAACTG GAGGACGGCA CCATGACGTG CCTGATGAAC 
GGAAATGGGC AGGTGAAGCG TCGCAAACGC CTGATCTCGA ACGAGTCCGG CGACTCGATC 
GACTCCAGTT CCACGGAGAA GAAAACGCCC AAGATGCCCG ACGGTGGATA TGGGTGGGTG 
GTGGTGTTCG CCTCTCTGGT CGTGTCCCTT ATAGCCGACG GACTGAGCTT CTCATTTGGC 
CTAATAAACG TGGAACTGCT GGAATACTTC GGAGAGTCCA CTTCGAAGAC CGCCTGGATA 
TCCTCGCTAT TCTTCTCCGT GCCCCTGTTG ATGGGACCCA TCTGGTCCAA TCTGGTGGAC 
AAGTACGGCT GCCGAAAGAT GACCATCCTC GGCGGAGTGG TCTCTGCCTT TGGGTTTGCT 
CTGTCTTCGT TTTGCAACTC GATCGAGATG CTCATGGTGA CCTTTGGTAT CATTTCGGGA 
CTGGGCCTAG GCATCGGGTA TGTGACCGCG GTGGTGTCCA TTGCATTCTG GTTTGACAAG 
AAGCGCACCT TTGCGACCGG CATCGGAGCA TCGGGCACCG GCATCGGGAC CTTTGTCTAC 
GCCCGCCTTA CTTCCTATCT AATCGAATCG TACGGCTGGC GCGGAGCCAC TCTAATCCTT 
GGCGGCACCA TGCTCAACGC ATGCGTTTGC GGCGCCCTCA TGCGCGATCC CGACTGGCTG 
ATCGAGGAAA ACCGTCTAGA GAGTCGATCC CAAAGCGTCA CCACCTTCTC GAACTCCAGT 
GTCTGCCTGG AGGAAATAAA AAAGCTGCTG GACACCGGCA TCACAAAGGA GGCTGTGCTA 
GACTCGTTGG TGACCAAGAA CAACACGGAG GCCAACCAGC AGATTGACGA TCCGTTGGAC 
TCCGCTCTCA AGCGCTACCG CAGCGAGATC TTCCTGCCCA CCTTCCTGAG CACTCAGGAG 
CTTGACAGCA TCTGCGAGGT TAAGAGCCTG AGCCGACGCT CCCTACGCCA CAAGGAGGGT 
GAGGAGGCGC CGTCACGAGA GAACCTGCTG TCCATGTCCT CAGGAGCAGG TGCCTATCCC 
CCATCGACGG CCGTTATAGG CTCGCCAGAT GATACCCTAA TGGGCGGAAT AGCTCACGAG 
GCGGCAGAGG AAGCAAAGAA GGCCTATCTG GCCTCCATCG AGACGCTGTC TCCATCGGAA 
AAGCGCTCCA CTGGTAGGAC TCCCAATGGC TCGCTCCGCT CCTCGGACGA AGGCTACCTT 
ACGCAGAAGC ACGCCAGCTC CCGGTACTCG CTTAACGAAA ACATCTTCAT GGCCAAGCAT 
ACAACACCAT CAATCTCCAA CCTAAAGGTG AATGGCCTGC GACACAATTC GGTGGACATC 
TTGAGCGAGG ACATGCACTG CTACTACTCG AAGGACGAGA CCTTCGCATT GCTGGAGCCG 
AGCAGGCGCA TCAGGCCCAC CGTCATCGCC ATTCCGGAAC AGGAGCAGTC GGTGAACAGC 
GAGCTGGCCA CCCGTCGCGC ACGCCTGGAC AGCATCACGG GAATCCGCCG GCTGTCGCGC 
TCCAAGAAGC CCAGCCACCA CCGATCGAAC CTCCGCCGCA ACATCTCGAT CCGGAACTCC 
AACTTCCTTA AGGATATGCG CATTCACCGC AACTCGATCC ACTACCGCGG GGCCATGCTG 
AACACGCACC GCTACCGGCT GCGCGCCTCC TCCTGTCCCA ACATCTACCG CAACTCGATG 
ACCACCATCG CCAAAGAGGA GGAAGATACC TGGTATGACA ACTTTGTGGA CACCATGAAG 
TCCATCTTTG ACTTTTCACT CTTTCTGGAC ATGAAGTTCG CCCTCTTCAA TCTCTCTACA 
TTGTTTTTGT TCATTTGGTT CATTATCCCC TATCTGTACC TTCCCGATTA CATGAAGCAA 
TACAAGTACG ACGTGAGCCA GAGTGCCGAG TTGATTTCGG ATGTCGGCAT TGCCCAAACG 
GTGGGCATGA TAGGACTTGG CTATCTGGGG GATCTGTCCT GGATGAACAT CAATATATGC 
TACTCGCTGT GCATGCTGGT GTGCGGAGCG TCTGTGTTCT TCATGCCCCT GCTGATTACC 
AACTACATGG GCCTGATGGT CATGTGCGTT ATTTTCGGAT TTACGTTCGC CAGCTCCTTC 
TCGTTTACGC CCAGCATTCT GGTCAGTATT GTGGATCTGG ACGATTTCAC GTGTGCCTAC 
GGTCTGGTGC TGCTGGTTCA GGGAGTTGGA ATGATCGCAG GACCCCCTAT TGCAGGCGTC 
ATATACGAGT ATACGGGCAG ATGGGATGAC ACCTTCTATT ATGCTGGAAT ATTTATCGCA 
CTCTCTGGTG TCTGTTCGTA TCTGATCGAG TTCTGTGAAA AGAAAGCACC TAAGGAGAGT 
GATAGTGATG TCTTAGAGAC TAAAAAAGCT CAACTTTTAC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163063.1
CDS	1490..4174
Translation	MAKLATDPNASNNNSCEAVNNNHNGQNQSQNGGASNYQALPLTPAPANTPLHKAIKHDLFPEVTFCNLSVEELADGAGHSRVVRSSVIELEDGTMTCLMNGNGQVKRRKRLISNESGDSIDSSSTEKKTPKMPDGGYGWVVVFASLVVSLIADGLSFSFGLINVELLEYFGESTSKTAWISSLFFSVPLLMGPIWSNLVDKYGCRKMTILGGVVSAFGFALSSFCNSIEMLMVTFGIISGLGLGIGYVTAVVSIAFWFDKKRTFATGIGASGTGIGTFVYARLTSYLIESYGWRGATLILGGTMLNACVCGALMRDPDWLIEENRLESRSQSVTTFSNSSVCLEEIKKLLDTGITKEAVLDSLVTKNNTEANQQIDDPLDSALKRYRSEIFLPTFLSTQELDSICEVKSLSRRSLRHKEGEEAPSRENLLSMSSGAGAYPPSTAVIGSPDDTLMGGIAHEAAEEAKKAYLASIETLSPSEKRSTGRTPNGSLRSSDEGYLTQKHASSRYSLNENIFMAKHTTPSISNLKVNGLRHNSVDILSEDMHCYYSKDETFALLEPSRRIRPTVIAIPEQEQSVNSELATRRARLDSITGIRRLSRSKKPSHHRSNLRRNISIRNSNFLKDMRIHRNSIHYRGAMLNTHRYRLRASSCPNIYRNSMTTIAKEEEDTWYDNFVDTMKSIFDFSLFLDMKFALFNLSTLFLFIWFIIPYLYLPDYMKQYKYDVSQSAELISDVGIAQTVGMIGLGYLGDLSWMNINICYSLCMLVCGASVFFMPLLITNYMGLMVMCVIFGFTFASSFSFTPSILVSIVDLDDFTCAYGLVLLVQGVGMIAGPPIAGVIYEYTGRWDDTFYYAGIFIALSGVCSYLIEFCEKKAPKESDSDVLETKKAQLLH

Target ORF information:

RefSeq Version	NM_001169592.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chaski (chk), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169592.2

ORF Insert Sequence:

ATGGCCAAGC TAGCGACGGA TCCAAACGCC TCCAACAACA ACAGCTGCGA GGCGGTTAAC 
AACAACCACA ATGGCCAGAA CCAGAGCCAA AACGGAGGCG CCTCCAATTA CCAGGCCCTG 
CCTCTTACGC CTGCTCCGGC CAACACGCCA CTCCACAAGG CCATCAAGCA CGACCTCTTC 
CCGGAGGTCA CCTTCTGCAA CCTCTCCGTA GAGGAGCTCG CAGATGGGGC TGGACACAGC 
CGTGTGGTAA GGAGCAGTGT CATCGAACTG GAGGACGGCA CCATGACGTG CCTGATGAAC 
GGAAATGGGC AGGTGAAGCG TCGCAAACGC CTGATCTCGA ACGAGTCCGG CGACTCGATC 
GACTCCAGTT CCACGGAGAA GAAAACGCCC AAGATGCCCG ACGGTGGATA TGGGTGGGTG 
GTGGTGTTCG CCTCTCTGGT CGTGTCCCTT ATAGCCGACG GACTGAGCTT CTCATTTGGC 
CTAATAAACG TGGAACTGCT GGAATACTTC GGAGAGTCCA CTTCGAAGAC CGCCTGGATA 
TCCTCGCTAT TCTTCTCCGT GCCCCTGTTG ATGGGACCCA TCTGGTCCAA TCTGGTGGAC 
AAGTACGGCT GCCGAAAGAT GACCATCCTC GGCGGAGTGG TCTCTGCCTT TGGGTTTGCT 
CTGTCTTCGT TTTGCAACTC GATCGAGATG CTCATGGTGA CCTTTGGTAT CATTTCGGGA 
CTGGGCCTAG GCATCGGGTA TGTGACCGCG GTGGTGTCCA TTGCATTCTG GTTTGACAAG 
AAGCGCACCT TTGCGACCGG CATCGGAGCA TCGGGCACCG GCATCGGGAC CTTTGTCTAC 
GCCCGCCTTA CTTCCTATCT AATCGAATCG TACGGCTGGC GCGGAGCCAC TCTAATCCTT 
GGCGGCACCA TGCTCAACGC ATGCGTTTGC GGCGCCCTCA TGCGCGATCC CGACTGGCTG 
ATCGAGGAAA ACCGTCTAGA GAGTCGATCC CAAAGCGTCA CCACCTTCTC GAACTCCAGT 
GTCTGCCTGG AGGAAATAAA AAAGCTGCTG GACACCGGCA TCACAAAGGA GGCTGTGCTA 
GACTCGTTGG TGACCAAGAA CAACACGGAG GCCAACCAGC AGATTGACGA TCCGTTGGAC 
TCCGCTCTCA AGCGCTACCG CAGCGAGATC TTCCTGCCCA CCTTCCTGAG CACTCAGGAG 
CTTGACAGCA TCTGCGAGGT TAAGAGCCTG AGCCGACGCT CCCTACGCCA CAAGGAGGGT 
GAGGAGGCGC CGTCACGAGA GAACCTGCTG TCCATGTCCT CAGGAGCAGG TGCCTATCCC 
CCATCGACGG CCGTTATAGG CTCGCCAGAT GATACCCTAA TGGGCGGAAT AGCTCACGAG 
GCGGCAGAGG AAGCAAAGAA GGCCTATCTG GCCTCCATCG AGACGCTGTC TCCATCGGAA 
AAGCGCTCCA CTGGTAGGAC TCCCAATGGC TCGCTCCGCT CCTCGGACGA AGGCTACCTT 
ACGCAGAAGC ACGCCAGCTC CCGGTACTCG CTTAACGAAA ACATCTTCAT GGCCAAGCAT 
ACAACACCAT CAATCTCCAA CCTAAAGGTG AATGGCCTGC GACACAATTC GGTGGACATC 
TTGAGCGAGG ACATGCACTG CTACTACTCG AAGGACGAGA CCTTCGCATT GCTGGAGCCG 
AGCAGGCGCA TCAGGCCCAC CGTCATCGCC ATTCCGGAAC AGGAGCAGTC GGTGAACAGC 
GAGCTGGCCA CCCGTCGCGC ACGCCTGGAC AGCATCACGG GAATCCGCCG GCTGTCGCGC 
TCCAAGAAGC CCAGCCACCA CCGATCGAAC CTCCGCCGCA ACATCTCGAT CCGGAACTCC 
AACTTCCTTA AGGATATGCG CATTCACCGC AACTCGATCC ACTACCGCGG GGCCATGCTG 
AACACGCACC GCTACCGGCT GCGCGCCTCC TCCTGTCCCA ACATCTACCG CAACTCGATG 
ACCACCATCG CCAAAGAGGA GGAAGATACC TGGTATGACA ACTTTGTGGA CACCATGAAG 
TCCATCTTTG ACTTTTCACT CTTTCTGGAC ATGAAGTTCG CCCTCTTCAA TCTCTCTACA 
TTGTTTTTGT TCATTTGGTT CATTATCCCC TATCTGTACC TTCCCGATTA CATGAAGCAA 
TACAAGTACG ACGTGAGCCA GAGTGCCGAG TTGATTTCGG ATGTCGGCAT TGCCCAAACG 
GTGGGCATGA TAGGACTTGG CTATCTGGGG GATCTGTCCT GGATGAACAT CAATATATGC 
TACTCGCTGT GCATGCTGGT GTGCGGAGCG TCTGTGTTCT TCATGCCCCT GCTGATTACC 
AACTACATGG GCCTGATGGT CATGTGCGTT ATTTTCGGAT TTACGTTCGC CAGCTCCTTC 
TCGTTTACGC CCAGCATTCT GGTCAGTATT GTGGATCTGG ACGATTTCAC GTGTGCCTAC 
GGTCTGGTGC TGCTGGTTCA GGGAGTTGGA ATGATCGCAG GACCCCCTAT TGCAGGCGTC 
ATATACGAGT ATACGGGCAG ATGGGATGAC ACCTTCTATT ATGCTGGAAT ATTTATCGCA 
CTCTCTGGTG TCTGTTCGTA TCTGATCGAG TTCTGTGAAA AGAAAGCACC TAAGGAGAGT 
GATAGTGATG TCTTAGAGAC TAAAAAAGCT CAACTTTTAC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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chk cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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