Hsepi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsepi
Entrez Gene ID	35569
Full Name	Heparan sulfate C5-epimerase
Synonyms	CG3194,Dmel\CG3194
General protein information	Preferred Names Heparan sulfate C5-epimerase Names CG3194 gene product from transcript CG3194-RB C5-epimerase CG3194-PA CG3194-PB Hsepi-PA Hsepi-PB heparan sulfate C5-epimerase heparan sulfate glucuronyl C5-epimerase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42C4-42C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169591.2	NP_001163062.1	heparan sulfate C5-epimerase, isoform B
NM_136405.4	NP_610249.2	heparan sulfate C5-epimerase, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Glce	NP_201577.3
Danio rerio (zebrafish)	glceb	NP_998014.1
	GLCE	XP_001085618.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005699	XP_315706.4
Caenorhabditis elegans (roundworm)	hse-5	NP_497876.2
Canis lupus familiaris (dog)	GLCE	XP_005638597.1
Rattus norvegicus (Norway rat)	Glce	XP_343405.2
Drosophila melanogaster (fruit fly)	Hsepi	NP_001163062.1
Xenopus tropicalis (tropical clawed frog)	glce	XP_002932993.1
Pan troglodytes (chimpanzee)	GLCE	XP_001174876.1
Bos taurus (cattle)	GLCE	NP_776495.1
Gallus gallus (chicken)	GLCE	XP_003641912.1
Homo sapiens (human)	GLCE	NP_056369.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This result suggests that the increase in 6-O-sulfation in Hsepi mutants is critical for the rescue of FGF signaling
Title: Analysis of Drosophila glucuronyl C5-epimerase: implications for developmental roles of heparan sulfate sulfation compensation and 2-O-sulfated glucuronic acid.

The following Hsepi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsepi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09039	NM_136405.4 Latest version!	Drosophila melanogaster heparan sulfate C5-epimerase, transcript variant A (Hsepi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12218	NM_001169591.2 Latest version!	Drosophila melanogaster heparan sulfate C5-epimerase, transcript variant B (Hsepi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsepi ( NM_136405.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610249.2 Drosophila melanogaster heparan sulfate C5-epimerase, transcript variant A (Hsepi), mRNA.

Price & Availability↑

CloneID	OFa09039
Clone ID Related Accession (Same CDS sequence)	NM_136405.4
Accession Version	NM_136405.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heparan sulfate C5-epimerase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136405.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAAT ATCTAAGCAG TCAGAGAGAT GCCCTTTCCG CACCCGCGCT TCCAGTAAGC 
AGGGAAAACC GGGAGCCACC TAAATTCCAA GGGGTAAAGC AACGCGAGCC ACTGGTCTTT 
TTCATCATGC GACTGAACCT AAAAGCCGTG CTGCTTGTCC TCACAGTGGC TGTGGTGGTA 
ATCACTCTGG GCGTGTATAT GCGCTGCGCC GCCTTCTCCT TTTCGCCGGA CTTTGTGCGT 
CCTCTGGACC GGTCCGCAAG GCAGTCCTCC AGCGGAGGAG AAGCTACAGC GCTGCACGAC 
ATTGAGTGCT CCATTAACCA GGAGTACACC GTGCACTGCA AGCGAGACGA GAACGCCAAC 
GAGGTTTACG TTCCGTTTTC CTTCCTGCGT AACTACTTCG ACGTCAGTGG AGCAGTTTCC 
ACCAACAGCA ATGAGGTGGC CAAGTTCAAC TGGGTGCACA GCACAGCCAA AGTTAACCTT 
CCCAGGGGAA AGTACGATGC GCGCGGCGTC TATATGTACT TTGAGAACTA CAACGTGGAA 
GTGCGCGACC GGGTAAAGTG CATCAGTGCC GCGGAGGGCG TTCCAGTGAG CACGCAATGG 
GAGAAGCGTG GGTACTTTTA CCCCACACAA ATTGCCCAGT TCGCGTTGTC GCACTACAGC 
AAGAACCTCA CAGAGCCGGC ACCCAGGGTT CGCGTGTTGG AGGACGGCGA CGGGAACCAG 
ATGGAATGGA GTACGCCCAA GACCAGCAAC ATGACCCGCA TCTGGCACCA CAAGTTTAAC 
ACCAGTGTTG TCCAGTTCGA GACGGCACCT GGTTACGAAG GCGTCATCAG CATTGCTCTA 
AACCAGACAT TGGATCTTCT GCTCAGCGTT GATCTGCTCC TGGTAACCAA TAGCAGTAGT 
CTCATGATTA CCGTTCAGAA TCGGGACACC CGTCACAACT ACAGCCTGCA CTACATACCC 
GCAGATCTGC TGCTGAGCGT GCAGGATACA AACATCTACT ACGGTCTGGG AGGCTCGGCA 
CTGAACAAGT GGCGCCACAT CACTCGAGAC CTGCACATTG ACCTTCAAAA GGGCATCATG 
GGTGACAAAC GCTCGCCGCT AAAGATACGG CGCTCCGATC TGGAGGTAAT ATCTATTGGC 
TTTCTGGGCC TTGGCTTCTT CGACAATATC ACCTTGTCCA CCAGTGACCA TCTGGCTCAC 
TTCTACGACG CGGCTGAGTG GTTCGTCCAC AACCAGGACC CCAAGACGGG TGGCTGGACC 
AATCCTGTGC GTCGCAGCCT CAACGGCTTT GCCGAATTGC GTCCGGGGTG GATATCAGCC 
ATGGGCCAGG GACACGCTAT TTCAGTGCTG GCACGAGCCT ACTGGCACTC CGGCGGGGAT 
GAACGCTACC TTCGAGCGGC CGCAGCCGGA CTGCAGCCTT ATAGGGTATA CTCGCGCGAT 
GGCGGCGTTC TGGCGCAGTT CATGGACAAG TTCTACTGGT ACGAGGAGTA CCCCACCACG 
CCACCCTCTT ATGTGCTAAA CGGCTTTATC TATTCACTGC TGGGTCTCTA CGATCTCAAC 
AGCACTGCCC CGGGAAAAAT CGCCCGCGAG GCTGGAAAGC TTTTCGCGCA GGGCATGCAC 
TCCCTTAAAA AGATGCTGTT GCTATTTGAC ACTGGCTCCG GCACCAGCTA CGACCTGCGC 
CATCTGAGCC TGGGCGTAGC TCCTAATCTG GCGCGCTGGG ACTACCATGC AACGCACGTG 
AACCAGTTAC TTCTGTTAGC CACCATCGAC AGCGATCCAT TAATTGCACA AACTGCGGAG 
CGCTGGAAGG GCTATATGTT TGGTCGTCGG GCCAAGCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610249.2
CDS	332..2176
Translation	MSKYLSSQRDALSAPALPVSRENREPPKFQGVKQREPLVFFIMRLNLKAVLLVLTVAVVVITLGVYMRCAAFSFSPDFVRPLDRSARQSSSGGEATALHDIECSINQEYTVHCKRDENANEVYVPFSFLRNYFDVSGAVSTNSNEVAKFNWVHSTAKVNLPRGKYDARGVYMYFENYNVEVRDRVKCISAAEGVPVSTQWEKRGYFYPTQIAQFALSHYSKNLTEPAPRVRVLEDGDGNQMEWSTPKTSNMTRIWHHKFNTSVVQFETAPGYEGVISIALNQTLDLLLSVDLLLVTNSSSLMITVQNRDTRHNYSLHYIPADLLLSVQDTNIYYGLGGSALNKWRHITRDLHIDLQKGIMGDKRSPLKIRRSDLEVISIGFLGLGFFDNITLSTSDHLAHFYDAAEWFVHNQDPKTGGWTNPVRRSLNGFAELRPGWISAMGQGHAISVLARAYWHSGGDERYLRAAAAGLQPYRVYSRDGGVLAQFMDKFYWYEEYPTTPPSYVLNGFIYSLLGLYDLNSTAPGKIAREAGKLFAQGMHSLKKMLLLFDTGSGTSYDLRHLSLGVAPNLARWDYHATHVNQLLLLATIDSDPLIAQTAERWKGYMFGRRAKHN

Target ORF information:

RefSeq Version	NM_136405.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heparan sulfate C5-epimerase, transcript variant A (Hsepi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136405.4

ORF Insert Sequence:

ATGTCCAAAT ATCTAAGCAG TCAGAGAGAT GCCCTTTCCG CACCCGCGCT TCCAGTAAGC 
AGGGAAAACC GGGAGCCACC TAAATTCCAA GGGGTAAAGC AACGCGAGCC ACTGGTCTTT 
TTCATCATGC GACTGAACCT AAAAGCCGTG CTGCTTGTCC TCACAGTGGC TGTGGTGGTA 
ATCACTCTGG GCGTGTATAT GCGCTGCGCC GCCTTCTCCT TTTCGCCGGA CTTTGTGCGT 
CCTCTGGACC GGTCCGCAAG GCAGTCCTCC AGCGGAGGAG AAGCTACAGC GCTGCACGAC 
ATTGAGTGCT CCATTAACCA GGAGTACACC GTGCACTGCA AGCGAGACGA GAACGCCAAC 
GAGGTTTACG TTCCGTTTTC CTTCCTGCGT AACTACTTCG ACGTCAGTGG AGCAGTTTCC 
ACCAACAGCA ATGAGGTGGC CAAGTTCAAC TGGGTGCACA GCACAGCCAA AGTTAACCTT 
CCCAGGGGAA AGTACGATGC GCGCGGCGTC TATATGTACT TTGAGAACTA CAACGTGGAA 
GTGCGCGACC GGGTAAAGTG CATCAGTGCC GCGGAGGGCG TTCCAGTGAG CACGCAATGG 
GAGAAGCGTG GGTACTTTTA CCCCACACAA ATTGCCCAGT TCGCGTTGTC GCACTACAGC 
AAGAACCTCA CAGAGCCGGC ACCCAGGGTT CGCGTGTTGG AGGACGGCGA CGGGAACCAG 
ATGGAATGGA GTACGCCCAA GACCAGCAAC ATGACCCGCA TCTGGCACCA CAAGTTTAAC 
ACCAGTGTTG TCCAGTTCGA GACGGCACCT GGTTACGAAG GCGTCATCAG CATTGCTCTA 
AACCAGACAT TGGATCTTCT GCTCAGCGTT GATCTGCTCC TGGTAACCAA TAGCAGTAGT 
CTCATGATTA CCGTTCAGAA TCGGGACACC CGTCACAACT ACAGCCTGCA CTACATACCC 
GCAGATCTGC TGCTGAGCGT GCAGGATACA AACATCTACT ACGGTCTGGG AGGCTCGGCA 
CTGAACAAGT GGCGCCACAT CACTCGAGAC CTGCACATTG ACCTTCAAAA GGGCATCATG 
GGTGACAAAC GCTCGCCGCT AAAGATACGG CGCTCCGATC TGGAGGTAAT ATCTATTGGC 
TTTCTGGGCC TTGGCTTCTT CGACAATATC ACCTTGTCCA CCAGTGACCA TCTGGCTCAC 
TTCTACGACG CGGCTGAGTG GTTCGTCCAC AACCAGGACC CCAAGACGGG TGGCTGGACC 
AATCCTGTGC GTCGCAGCCT CAACGGCTTT GCCGAATTGC GTCCGGGGTG GATATCAGCC 
ATGGGCCAGG GACACGCTAT TTCAGTGCTG GCACGAGCCT ACTGGCACTC CGGCGGGGAT 
GAACGCTACC TTCGAGCGGC CGCAGCCGGA CTGCAGCCTT ATAGGGTATA CTCGCGCGAT 
GGCGGCGTTC TGGCGCAGTT CATGGACAAG TTCTACTGGT ACGAGGAGTA CCCCACCACG 
CCACCCTCTT ATGTGCTAAA CGGCTTTATC TATTCACTGC TGGGTCTCTA CGATCTCAAC 
AGCACTGCCC CGGGAAAAAT CGCCCGCGAG GCTGGAAAGC TTTTCGCGCA GGGCATGCAC 
TCCCTTAAAA AGATGCTGTT GCTATTTGAC ACTGGCTCCG GCACCAGCTA CGACCTGCGC 
CATCTGAGCC TGGGCGTAGC TCCTAATCTG GCGCGCTGGG ACTACCATGC AACGCACGTG 
AACCAGTTAC TTCTGTTAGC CACCATCGAC AGCGATCCAT TAATTGCACA AACTGCGGAG 
CGCTGGAAGG GCTATATGTT TGGTCGTCGG GCCAAGCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsepi ( NM_001169591.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163062.1 Drosophila melanogaster heparan sulfate C5-epimerase, transcript variant B (Hsepi), mRNA.

Price & Availability↑

CloneID	OFa12218
Clone ID Related Accession (Same CDS sequence)	NM_001169591.2
Accession Version	NM_001169591.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heparan sulfate C5-epimerase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169591.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAAT ATCTAAGCAG TCAGAGAGAT GCCCTTTCCG CACCCGCGCT TCCAGTAAGC 
AGGGAAAACC GGGAGCCACC TAAATTCCAA GGGCAACGCG AGCCACTGGT CTTTTTCATC 
ATGCGACTGA ACCTAAAAGC CGTGCTGCTT GTCCTCACAG TGGCTGTGGT GGTAATCACT 
CTGGGCGTGT ATATGCGCTG CGCCGCCTTC TCCTTTTCGC CGGACTTTGT GCGTCCTCTG 
GACCGGTCCG CAAGGCAGTC CTCCAGCGGA GGAGAAGCTA CAGCGCTGCA CGACATTGAG 
TGCTCCATTA ACCAGGAGTA CACCGTGCAC TGCAAGCGAG ACGAGAACGC CAACGAGGTT 
TACGTTCCGT TTTCCTTCCT GCGTAACTAC TTCGACGTCA GTGGAGCAGT TTCCACCAAC 
AGCAATGAGG TGGCCAAGTT CAACTGGGTG CACAGCACAG CCAAAGTTAA CCTTCCCAGG 
GGAAAGTACG ATGCGCGCGG CGTCTATATG TACTTTGAGA ACTACAACGT GGAAGTGCGC 
GACCGGGTAA AGTGCATCAG TGCCGCGGAG GGCGTTCCAG TGAGCACGCA ATGGGAGAAG 
CGTGGGTACT TTTACCCCAC ACAAATTGCC CAGTTCGCGT TGTCGCACTA CAGCAAGAAC 
CTCACAGAGC CGGCACCCAG GGTTCGCGTG TTGGAGGACG GCGACGGGAA CCAGATGGAA 
TGGAGTACGC CCAAGACCAG CAACATGACC CGCATCTGGC ACCACAAGTT TAACACCAGT 
GTTGTCCAGT TCGAGACGGC ACCTGGTTAC GAAGGCGTCA TCAGCATTGC TCTAAACCAG 
ACATTGGATC TTCTGCTCAG CGTTGATCTG CTCCTGGTAA CCAATAGCAG TAGTCTCATG 
ATTACCGTTC AGAATCGGGA CACCCGTCAC AACTACAGCC TGCACTACAT ACCCGCAGAT 
CTGCTGCTGA GCGTGCAGGA TACAAACATC TACTACGGTC TGGGAGGCTC GGCACTGAAC 
AAGTGGCGCC ACATCACTCG AGACCTGCAC ATTGACCTTC AAAAGGGCAT CATGGGTGAC 
AAACGCTCGC CGCTAAAGAT ACGGCGCTCC GATCTGGAGG TAATATCTAT TGGCTTTCTG 
GGCCTTGGCT TCTTCGACAA TATCACCTTG TCCACCAGTG ACCATCTGGC TCACTTCTAC 
GACGCGGCTG AGTGGTTCGT CCACAACCAG GACCCCAAGA CGGGTGGCTG GACCAATCCT 
GTGCGTCGCA GCCTCAACGG CTTTGCCGAA TTGCGTCCGG GGTGGATATC AGCCATGGGC 
CAGGGACACG CTATTTCAGT GCTGGCACGA GCCTACTGGC ACTCCGGCGG GGATGAACGC 
TACCTTCGAG CGGCCGCAGC CGGACTGCAG CCTTATAGGG TATACTCGCG CGATGGCGGC 
GTTCTGGCGC AGTTCATGGA CAAGTTCTAC TGGTACGAGG AGTACCCCAC CACGCCACCC 
TCTTATGTGC TAAACGGCTT TATCTATTCA CTGCTGGGTC TCTACGATCT CAACAGCACT 
GCCCCGGGAA AAATCGCCCG CGAGGCTGGA AAGCTTTTCG CGCAGGGCAT GCACTCCCTT 
AAAAAGATGC TGTTGCTATT TGACACTGGC TCCGGCACCA GCTACGACCT GCGCCATCTG 
AGCCTGGGCG TAGCTCCTAA TCTGGCGCGC TGGGACTACC ATGCAACGCA CGTGAACCAG 
TTACTTCTGT TAGCCACCAT CGACAGCGAT CCATTAATTG CACAAACTGC GGAGCGCTGG 
AAGGGCTATA TGTTTGGTCG TCGGGCCAAG CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163062.1
CDS	332..2170
Translation	MSKYLSSQRDALSAPALPVSRENREPPKFQGQREPLVFFIMRLNLKAVLLVLTVAVVVITLGVYMRCAAFSFSPDFVRPLDRSARQSSSGGEATALHDIECSINQEYTVHCKRDENANEVYVPFSFLRNYFDVSGAVSTNSNEVAKFNWVHSTAKVNLPRGKYDARGVYMYFENYNVEVRDRVKCISAAEGVPVSTQWEKRGYFYPTQIAQFALSHYSKNLTEPAPRVRVLEDGDGNQMEWSTPKTSNMTRIWHHKFNTSVVQFETAPGYEGVISIALNQTLDLLLSVDLLLVTNSSSLMITVQNRDTRHNYSLHYIPADLLLSVQDTNIYYGLGGSALNKWRHITRDLHIDLQKGIMGDKRSPLKIRRSDLEVISIGFLGLGFFDNITLSTSDHLAHFYDAAEWFVHNQDPKTGGWTNPVRRSLNGFAELRPGWISAMGQGHAISVLARAYWHSGGDERYLRAAAAGLQPYRVYSRDGGVLAQFMDKFYWYEEYPTTPPSYVLNGFIYSLLGLYDLNSTAPGKIAREAGKLFAQGMHSLKKMLLLFDTGSGTSYDLRHLSLGVAPNLARWDYHATHVNQLLLLATIDSDPLIAQTAERWKGYMFGRRAKHN

Target ORF information:

RefSeq Version	NM_001169591.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heparan sulfate C5-epimerase, transcript variant B (Hsepi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169591.2

ORF Insert Sequence:

ATGTCCAAAT ATCTAAGCAG TCAGAGAGAT GCCCTTTCCG CACCCGCGCT TCCAGTAAGC 
AGGGAAAACC GGGAGCCACC TAAATTCCAA GGGCAACGCG AGCCACTGGT CTTTTTCATC 
ATGCGACTGA ACCTAAAAGC CGTGCTGCTT GTCCTCACAG TGGCTGTGGT GGTAATCACT 
CTGGGCGTGT ATATGCGCTG CGCCGCCTTC TCCTTTTCGC CGGACTTTGT GCGTCCTCTG 
GACCGGTCCG CAAGGCAGTC CTCCAGCGGA GGAGAAGCTA CAGCGCTGCA CGACATTGAG 
TGCTCCATTA ACCAGGAGTA CACCGTGCAC TGCAAGCGAG ACGAGAACGC CAACGAGGTT 
TACGTTCCGT TTTCCTTCCT GCGTAACTAC TTCGACGTCA GTGGAGCAGT TTCCACCAAC 
AGCAATGAGG TGGCCAAGTT CAACTGGGTG CACAGCACAG CCAAAGTTAA CCTTCCCAGG 
GGAAAGTACG ATGCGCGCGG CGTCTATATG TACTTTGAGA ACTACAACGT GGAAGTGCGC 
GACCGGGTAA AGTGCATCAG TGCCGCGGAG GGCGTTCCAG TGAGCACGCA ATGGGAGAAG 
CGTGGGTACT TTTACCCCAC ACAAATTGCC CAGTTCGCGT TGTCGCACTA CAGCAAGAAC 
CTCACAGAGC CGGCACCCAG GGTTCGCGTG TTGGAGGACG GCGACGGGAA CCAGATGGAA 
TGGAGTACGC CCAAGACCAG CAACATGACC CGCATCTGGC ACCACAAGTT TAACACCAGT 
GTTGTCCAGT TCGAGACGGC ACCTGGTTAC GAAGGCGTCA TCAGCATTGC TCTAAACCAG 
ACATTGGATC TTCTGCTCAG CGTTGATCTG CTCCTGGTAA CCAATAGCAG TAGTCTCATG 
ATTACCGTTC AGAATCGGGA CACCCGTCAC AACTACAGCC TGCACTACAT ACCCGCAGAT 
CTGCTGCTGA GCGTGCAGGA TACAAACATC TACTACGGTC TGGGAGGCTC GGCACTGAAC 
AAGTGGCGCC ACATCACTCG AGACCTGCAC ATTGACCTTC AAAAGGGCAT CATGGGTGAC 
AAACGCTCGC CGCTAAAGAT ACGGCGCTCC GATCTGGAGG TAATATCTAT TGGCTTTCTG 
GGCCTTGGCT TCTTCGACAA TATCACCTTG TCCACCAGTG ACCATCTGGC TCACTTCTAC 
GACGCGGCTG AGTGGTTCGT CCACAACCAG GACCCCAAGA CGGGTGGCTG GACCAATCCT 
GTGCGTCGCA GCCTCAACGG CTTTGCCGAA TTGCGTCCGG GGTGGATATC AGCCATGGGC 
CAGGGACACG CTATTTCAGT GCTGGCACGA GCCTACTGGC ACTCCGGCGG GGATGAACGC 
TACCTTCGAG CGGCCGCAGC CGGACTGCAG CCTTATAGGG TATACTCGCG CGATGGCGGC 
GTTCTGGCGC AGTTCATGGA CAAGTTCTAC TGGTACGAGG AGTACCCCAC CACGCCACCC 
TCTTATGTGC TAAACGGCTT TATCTATTCA CTGCTGGGTC TCTACGATCT CAACAGCACT 
GCCCCGGGAA AAATCGCCCG CGAGGCTGGA AAGCTTTTCG CGCAGGGCAT GCACTCCCTT 
AAAAAGATGC TGTTGCTATT TGACACTGGC TCCGGCACCA GCTACGACCT GCGCCATCTG 
AGCCTGGGCG TAGCTCCTAA TCTGGCGCGC TGGGACTACC ATGCAACGCA CGTGAACCAG 
TTACTTCTGT TAGCCACCAT CGACAGCGAT CCATTAATTG CACAAACTGC GGAGCGCTGG 
AAGGGCTATA TGTTTGGTCG TCGGGCCAAG CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hsepi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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