mle cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mle
Entrez Gene ID	35523
Full Name	maleless
Synonyms	CG11680,Dmel\CG11680,MLE,Mle,mak,mll,mls,nap,nap[ts]
General protein information	Preferred Names maleless Names CG11680 gene product from transcript CG11680-RC CG11680-PA CG11680-PC male lethal male-killer male-lethal maleless-no-action potential mle-PA mle-PC no action potential no-action-potential
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42A6-42A6\|2-55.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057293.4	NP_476641.1	maleless, isoform A
NM_165451.3	NP_724440.1	maleless, isoform C

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	dhx9	NP_001188373.1
Homo sapiens (human)	DHX9	NP_001348.2
Pan troglodytes (chimpanzee)	DHX9	XP_003308679.1
Canis lupus familiaris (dog)	DHX9	XP_005622469.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006599	XP_316626.4
Bos taurus (cattle)	DHX9	NP_776461.1
Mus musculus (house mouse)	Dhx9	NP_031868.2
Drosophila melanogaster (fruit fly)	mle	NP_476641.1
Rattus norvegicus (Norway rat)	Dhx9	NP_001100654.1
Xenopus tropicalis (tropical clawed frog)	dhx9	XP_002937403.1
	DHX9	XP_001114405.1
Caenorhabditis elegans (roundworm)	rha-1	NP_495890.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We have employed a systems biology approach (microarray) to investigate the global aneuploid effect of the maleless (mle) mutation that disrupts the binding of male specific lethal (MSL) proteins that function in dosage compensation.
Title: Drosophila maleless gene counteracts X global aneuploid effects in males.

MLE specifically targets hairpin RNAs at their site of transcription. The association of MLE at these sites is independent of sequence and chromosome location
Title: The Drosophila Helicase MLE Targets Hairpin Structures in Genomic Transcripts.

Structure of the RNA Helicase MLE Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling.
Title: Structure of the RNA Helicase MLE Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling.

The Drosophila Helicase MLE is Implicated in Functions Distinct From its Role in Dosage Compensation
Title: The Drosophila Helicase Maleless (MLE) is Implicated in Functions Distinct From its Role in Dosage Compensation.

UNR facilitates the interaction of MLE with the lncRNA roX2 during Drosophila dosage compensation.
Title: UNR facilitates the interaction of MLE with the lncRNA roX2 during Drosophila dosage compensation.

The following mle gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mle cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12412	NM_165451.3 Latest version!	Drosophila melanogaster maleless (mle), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa12413	NM_057293.4 Latest version!	Drosophila melanogaster maleless (mle), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mle ( NM_165451.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724440.1 Drosophila melanogaster maleless (mle), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa12412
Clone ID Related Accession (Same CDS sequence)	NM_165451.3
Accession Version	NM_165451.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	maleless, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165451.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTACG AACGCTCGCT GCGTGATCGC CGCCAAAACG ACAACGAGTA CCGACAATTC 
CTCGAGTTCC GCGAAAAGCT GCCCATTGCA GCGATGCGGT CGGAAATTTT AACCGCAATT 
AATGACAATC CAGTGGTAAT CATTCGTGGA AACACAGGGT GCGGAAAGAC TACCCAGATT 
GCCCAATACA TTCTTGATGA CTACATCTGC TCCGGCCAGG GCGGGTACGC TAATATTTAT 
GTGACCCAGC CTCGTCGCAT TTCAGCCATT TCTGTGGCCG AGCGCGTAGC TCGCGAGCGG 
TGCGAGCAAT TGGGCGACAC TGTAGGCTAC TCGGTGCGAT TTGAGTCTGT GTTTCCACGA 
CCCTACGGCG CAATCCTCTT TTGCACCGTG GGTGTGTTGC TGCGCAAGCT GGAGGCTGGC 
TTACGAGGAG TGTCGCATAT TATTGTGGAC GAAATCCACG AGAGAGACGT GAACAGCGAC 
TTTTTGCTGG TCATCCTTCG CGACATGGTC GACACATATC CGGACCTGCA TGTTATTCTT 
ATGTCGGCCA CTATTGATAC AACGAAATTT TCCAAATATT TTGGCATCTG TCCCGTATTG 
GAAGTACCCG GAAGAGCATT TCCCGTGCAG CAATTTTTCT TGGAGGACAT TATTCAGATG 
ACAGACTTTG TGCCTTCGGC GGAGTCGCGC CGAAAGCGCA AGGAAGTGGA GGACGAGGAG 
CAATTGCTTT CCGAGGACAA GGACGAGGCG GAAATCAACT ATAACAAGGT GTGCGAGGAC 
AAGTATTCAC AAAAGACTCG AAATGCTATG GCCATGCTAT CCGAATCGGA CGTTAGCTTT 
GAGCTGCTGG AGGCATTATT GATGCATATT AAGTCAAAAA ATATTCCCGG TGCTATCTTA 
GTATTCCTGC CCGGTTGGAA CCTGATCTTT GCGCTTATGA AGTTTCTGCA AAATACAAAC 
ATTTTTGGTG ATACATCCCA ATACCAAATT TTGCCGTGCC ACTCGCAGAT TCCACGAGAC 
GAGCAACGCA AGGTTTTCGA GCCTGTTCCA GAAGGCGTAA CCAAGATTAT ACTTTCTACC 
AACATTGCAG AGACTTCTAT AACTATTGAC GACATTGTTT TCGTAATTGA TATATGTAAG 
GCGCGCATGA AGCTCTTTAC TTCACATAAC AACCTAACCA GCTACGCCAC CGTTTGGGCA 
AGCAAAACCA ATTTGGAGCA GCGCAAGGGC CGAGCTGGAC GTGTCCGACC AGGCTTCTGC 
TTCACACTCT GTTCGCGTGC CCGCTTCCAA GCGCTAGAGG ACAATCTTAC GCCGGAGATG 
TTCCGCACAC CGTTGCACGA GATGGCGTTG ACCATTAAAT TACTCAGACT GGGCTCCATT 
CACCACTTTT TGTCAAAGGC ATTGGAGCCA CCTCCGGTAG ACGCAGTAAT CGAAGCTGAG 
GTGTTGCTTC GCGAGATGCG CTGCCTTGAC GCTAACGATG AACTTACTCC ATTGGGGCGC 
CTGCTGGCAC GCCTCCCAAT TGAACCAAGA CTTGGAAAGA TGATGGTCCT CGGCGCAGTA 
TTTGGCTGCG CCGATCTAAT GGCCATTATG GCTTCGTACT CTAGCACCTT CTCAGAGGTG 
TTTAGTCTTG ATATAGGTCA ACGTCGCCTG GCAAATCATC AGAAAGCGTT GAGTGGTACC 
AAATGTTCCG ACCACGTGGC AATGATTGTA GCTTCACAAA TGTGGCGGCG CGAGAAACAA 
CGCGGTGAGC ATATGGAAGC TCGGTTCTGT GACTGGAAAG GACTGCAAAT GAGTACTATG 
AACGTTATCT GGGATGCCAA GCAGCAGTTA CTGGATCTCC TTCAGCAGGC AGGATTCCCA 
GAAGAGTGTA TGATATCGCA CGAGGTAGAC GAAAGGATTG ATGGGGATGA CCCCGTGCTG 
GATGTGTCCC TGGCGCTGCT GTGCTTGGGT CTCTACCCCA ACATTTGCGT GCACAAGGAG 
AAGCGCAAAG TGCTGACTAC AGAGTCTAAA GCAGCGTTAC TGCACAAAAC CTCGGTGAAT 
TGCAGCAACT TGGCCGTCAC ATTTCCGTAT CCGTTCTTCG TTTTCGGCGA GAAGATTCGC 
ACGCGAGCTG TTTCCTGCAA GCAACTATCT ATGGTCTCGC CCTTGCAGGT GATATTGTTT 
GGATCGCGCA AGATTGACTT GGCCGCCAAC AATATAGTGC GCGTTGACAA CTGGCTTAAC 
TTCGACATAG AGCCGGAACT TGCGGCCAAG ATTGGTGCCC TTAAGCCGGC CCTGGAGGAT 
CTTATCACGG TGGCGTGCGA CAACCCTTCC GACATACTTC GCCTTGAGGA GCCCTATGCA 
CAGCTGGTCA AAGTGGTCAA AGACCTTTGC GTTAAGAGCG CCGGAGACTT CGGACTGCAG 
CGTGAATCAG GAATTCTGCC GCACCAATCA CGTCAGTTCA GTGACGGCGG AGGACCGCCC 
AAACGAGGTC GCTTTGAGAC AGGACGCTTT ACTAACAGCA GCTTTGGTCG CCGTGGAAAC 
GGCAGAACTT TTGGAGGAGG ATATGGAAAT AATGGAGGAG GATATGGAAA TAATGGAGGA 
GGATATGGAA ATATTGGAGG AGGATATGGA AATAATGCAG GAGGTTATGG AAATAATGGT 
GGATATGGAA ATAATGGCGG AGGTTATAGA AATAATGGTG GAGGATATGG AAACAATGGC 
GGAGGATATG GGAACAAGAG AGGTGGATTT GGAGACTCAT TTGAAAGCAA TAGAGGATCA 
GGCGGCGGCT TTAGAAATGG CGACCAAGGC GGCCGCTGGG GTAACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724440.1
CDS	880..3690
Translation	MDYERSLRDRRQNDNEYRQFLEFREKLPIAAMRSEILTAINDNPVVIIRGNTGCGKTTQIAQYILDDYICSGQGGYANIYVTQPRRISAISVAERVARERCEQLGDTVGYSVRFESVFPRPYGAILFCTVGVLLRKLEAGLRGVSHIIVDEIHERDVNSDFLLVILRDMVDTYPDLHVILMSATIDTTKFSKYFGICPVLEVPGRAFPVQQFFLEDIIQMTDFVPSAESRRKRKEVEDEEQLLSEDKDEAEINYNKVCEDKYSQKTRNAMAMLSESDVSFELLEALLMHIKSKNIPGAILVFLPGWNLIFALMKFLQNTNIFGDTSQYQILPCHSQIPRDEQRKVFEPVPEGVTKIILSTNIAETSITIDDIVFVIDICKARMKLFTSHNNLTSYATVWASKTNLEQRKGRAGRVRPGFCFTLCSRARFQALEDNLTPEMFRTPLHEMALTIKLLRLGSIHHFLSKALEPPPVDAVIEAEVLLREMRCLDANDELTPLGRLLARLPIEPRLGKMMVLGAVFGCADLMAIMASYSSTFSEVFSLDIGQRRLANHQKALSGTKCSDHVAMIVASQMWRREKQRGEHMEARFCDWKGLQMSTMNVIWDAKQQLLDLLQQAGFPEECMISHEVDERIDGDDPVLDVSLALLCLGLYPNICVHKEKRKVLTTESKAALLHKTSVNCSNLAVTFPYPFFVFGEKIRTRAVSCKQLSMVSPLQVILFGSRKIDLAANNIVRVDNWLNFDIEPELAAKIGALKPALEDLITVACDNPSDILRLEEPYAQLVKVVKDLCVKSAGDFGLQRESGILPHQSRQFSDGGGPPKRGRFETGRFTNSSFGRRGNGRTFGGGYGNNGGGYGNNGGGYGNIGGGYGNNAGGYGNNGGYGNNGGGYRNNGGGYGNNGGGYGNKRGGFGDSFESNRGSGGGFRNGDQGGRWGNF

Target ORF information:

RefSeq Version	NM_165451.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster maleless (mle), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165451.3

ORF Insert Sequence:

ATGGACTACG AACGCTCGCT GCGTGATCGC CGCCAAAACG ACAACGAGTA CCGACAATTC 
CTCGAGTTCC GCGAAAAGCT GCCCATTGCA GCGATGCGGT CGGAAATTTT AACCGCAATT 
AATGACAATC CAGTGGTAAT CATTCGTGGA AACACAGGGT GCGGAAAGAC TACCCAGATT 
GCCCAATACA TTCTTGATGA CTACATCTGC TCCGGCCAGG GCGGGTACGC TAATATTTAT 
GTGACCCAGC CTCGTCGCAT TTCAGCCATT TCTGTGGCCG AGCGCGTAGC TCGCGAGCGG 
TGCGAGCAAT TGGGCGACAC TGTAGGCTAC TCGGTGCGAT TTGAGTCTGT GTTTCCACGA 
CCCTACGGCG CAATCCTCTT TTGCACCGTG GGTGTGTTGC TGCGCAAGCT GGAGGCTGGC 
TTACGAGGAG TGTCGCATAT TATTGTGGAC GAAATCCACG AGAGAGACGT GAACAGCGAC 
TTTTTGCTGG TCATCCTTCG CGACATGGTC GACACATATC CGGACCTGCA TGTTATTCTT 
ATGTCGGCCA CTATTGATAC AACGAAATTT TCCAAATATT TTGGCATCTG TCCCGTATTG 
GAAGTACCCG GAAGAGCATT TCCCGTGCAG CAATTTTTCT TGGAGGACAT TATTCAGATG 
ACAGACTTTG TGCCTTCGGC GGAGTCGCGC CGAAAGCGCA AGGAAGTGGA GGACGAGGAG 
CAATTGCTTT CCGAGGACAA GGACGAGGCG GAAATCAACT ATAACAAGGT GTGCGAGGAC 
AAGTATTCAC AAAAGACTCG AAATGCTATG GCCATGCTAT CCGAATCGGA CGTTAGCTTT 
GAGCTGCTGG AGGCATTATT GATGCATATT AAGTCAAAAA ATATTCCCGG TGCTATCTTA 
GTATTCCTGC CCGGTTGGAA CCTGATCTTT GCGCTTATGA AGTTTCTGCA AAATACAAAC 
ATTTTTGGTG ATACATCCCA ATACCAAATT TTGCCGTGCC ACTCGCAGAT TCCACGAGAC 
GAGCAACGCA AGGTTTTCGA GCCTGTTCCA GAAGGCGTAA CCAAGATTAT ACTTTCTACC 
AACATTGCAG AGACTTCTAT AACTATTGAC GACATTGTTT TCGTAATTGA TATATGTAAG 
GCGCGCATGA AGCTCTTTAC TTCACATAAC AACCTAACCA GCTACGCCAC CGTTTGGGCA 
AGCAAAACCA ATTTGGAGCA GCGCAAGGGC CGAGCTGGAC GTGTCCGACC AGGCTTCTGC 
TTCACACTCT GTTCGCGTGC CCGCTTCCAA GCGCTAGAGG ACAATCTTAC GCCGGAGATG 
TTCCGCACAC CGTTGCACGA GATGGCGTTG ACCATTAAAT TACTCAGACT GGGCTCCATT 
CACCACTTTT TGTCAAAGGC ATTGGAGCCA CCTCCGGTAG ACGCAGTAAT CGAAGCTGAG 
GTGTTGCTTC GCGAGATGCG CTGCCTTGAC GCTAACGATG AACTTACTCC ATTGGGGCGC 
CTGCTGGCAC GCCTCCCAAT TGAACCAAGA CTTGGAAAGA TGATGGTCCT CGGCGCAGTA 
TTTGGCTGCG CCGATCTAAT GGCCATTATG GCTTCGTACT CTAGCACCTT CTCAGAGGTG 
TTTAGTCTTG ATATAGGTCA ACGTCGCCTG GCAAATCATC AGAAAGCGTT GAGTGGTACC 
AAATGTTCCG ACCACGTGGC AATGATTGTA GCTTCACAAA TGTGGCGGCG CGAGAAACAA 
CGCGGTGAGC ATATGGAAGC TCGGTTCTGT GACTGGAAAG GACTGCAAAT GAGTACTATG 
AACGTTATCT GGGATGCCAA GCAGCAGTTA CTGGATCTCC TTCAGCAGGC AGGATTCCCA 
GAAGAGTGTA TGATATCGCA CGAGGTAGAC GAAAGGATTG ATGGGGATGA CCCCGTGCTG 
GATGTGTCCC TGGCGCTGCT GTGCTTGGGT CTCTACCCCA ACATTTGCGT GCACAAGGAG 
AAGCGCAAAG TGCTGACTAC AGAGTCTAAA GCAGCGTTAC TGCACAAAAC CTCGGTGAAT 
TGCAGCAACT TGGCCGTCAC ATTTCCGTAT CCGTTCTTCG TTTTCGGCGA GAAGATTCGC 
ACGCGAGCTG TTTCCTGCAA GCAACTATCT ATGGTCTCGC CCTTGCAGGT GATATTGTTT 
GGATCGCGCA AGATTGACTT GGCCGCCAAC AATATAGTGC GCGTTGACAA CTGGCTTAAC 
TTCGACATAG AGCCGGAACT TGCGGCCAAG ATTGGTGCCC TTAAGCCGGC CCTGGAGGAT 
CTTATCACGG TGGCGTGCGA CAACCCTTCC GACATACTTC GCCTTGAGGA GCCCTATGCA 
CAGCTGGTCA AAGTGGTCAA AGACCTTTGC GTTAAGAGCG CCGGAGACTT CGGACTGCAG 
CGTGAATCAG GAATTCTGCC GCACCAATCA CGTCAGTTCA GTGACGGCGG AGGACCGCCC 
AAACGAGGTC GCTTTGAGAC AGGACGCTTT ACTAACAGCA GCTTTGGTCG CCGTGGAAAC 
GGCAGAACTT TTGGAGGAGG ATATGGAAAT AATGGAGGAG GATATGGAAA TAATGGAGGA 
GGATATGGAA ATATTGGAGG AGGATATGGA AATAATGCAG GAGGTTATGG AAATAATGGT 
GGATATGGAA ATAATGGCGG AGGTTATAGA AATAATGGTG GAGGATATGG AAACAATGGC 
GGAGGATATG GGAACAAGAG AGGTGGATTT GGAGACTCAT TTGAAAGCAA TAGAGGATCA 
GGCGGCGGCT TTAGAAATGG CGACCAAGGC GGCCGCTGGG GTAACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mle ( NM_057293.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476641.1 Drosophila melanogaster maleless (mle), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12413
Clone ID Related Accession (Same CDS sequence)	NM_057293.4
Accession Version	NM_057293.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	maleless, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057293.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATATAA AATCTTTTTT GTACCAATTT TGTGCTAAGT CGCAAATAGA ACCAAAGTTT 
GATATTCGCC AAACGGGTCC CAAAAATCGC CAGCGGTTCC TTTGCGAGGT TCGTGTAGAG 
CCAAATACCT ATATTGGTGT GGGTAATTCC ACTAACAAAA AGGATGCCGA GAAAAATGCG 
TGCCGTGATT TTGTCAACTA CCTAGTGCGC GTGGGCAAAC TGAACACCAA TGATGTTCCA 
GCGGATGCTG GTGCTTCGGG GGGCGGACCT AGGACTGGTC TGGAGGGTGC GGGCATGGCA 
GGGGGCAGTG GACAGCAGAA GCGAGTGTTT GATGGTCAGT CGGGTCCACA GGATCTGGGT 
GAGGCCTATC GCCCTCTAAA CCACGATGGC GGTGACGGCG GTAACCGCTA CTCAGTAATT 
GATCGCATCC AGGAGCAGAG GGACATGAAC GAAGCGGAGG CTTTCGATGT CAACGCTGCC 
ATCCACGGTA ACTGGACCAT AGAGAATGCC AAAGAGCGAC TGAACATTTA CAAGCAGACG 
AACAACATTC GCGACGATTA CAAATATACA CCAGTGGGCC CGGAACACGC TAGGAGCTTT 
TTGGCCGAAT TGTCCATTTA TGTGCCGGCG CTCAATCGCA CGGTGACGGC CCGTGAGTCG 
GGTTCAAACA AGAAGTCTGC CAGCAAGTCA TGCGCCCTGT CCCTGGTGCG CCAACTTTTC 
CATTTAAATG TGATAGAGCC CTTTAGTGGA ACTCTGAAGA AAAAAAAGGA TGAGCAGCTG 
AAGCCATATC CGGTGAAGCT TTCGCCAAAT CTGATAAACA AAATTGATGA GGTGATTAAG 
GGTTTGGACC TTCCTGTCGT AAACCCGCGC AATATCAAAA TCGAACTGGA CGGTCCTCCG 
ATTCCTCTCA TTGTGAACTT AAGCCGTATA GATTCCTCGC AACAGGATGG CGAAAAACGT 
CAGGAAAGCT CCGTGATTCC GTGGGCTCCT CCTCAGGCAA ACTGGAATAC GTGGCATGCC 
TGCAACATCG ACGAAGGCGA ACTGGCAACC ACCTCTATTG ATGACCTTTC GATGGACTAC 
GAACGCTCGC TGCGTGATCG CCGCCAAAAC GACAACGAGT ACCGACAATT CCTCGAGTTC 
CGCGAAAAGC TGCCCATTGC AGCGATGCGG TCGGAAATTT TAACCGCAAT TAATGACAAT 
CCAGTGGTAA TCATTCGTGG AAACACAGGG TGCGGAAAGA CTACCCAGAT TGCCCAATAC 
ATTCTTGATG ACTACATCTG CTCCGGCCAG GGCGGGTACG CTAATATTTA TGTGACCCAG 
CCTCGTCGCA TTTCAGCCAT TTCTGTGGCC GAGCGCGTAG CTCGCGAGCG GTGCGAGCAA 
TTGGGCGACA CTGTAGGCTA CTCGGTGCGA TTTGAGTCTG TGTTTCCACG ACCCTACGGC 
GCAATCCTCT TTTGCACCGT GGGTGTGTTG CTGCGCAAGC TGGAGGCTGG CTTACGAGGA 
GTGTCGCATA TTATTGTGGA CGAAATCCAC GAGAGAGACG TGAACAGCGA CTTTTTGCTG 
GTCATCCTTC GCGACATGGT CGACACATAT CCGGACCTGC ATGTTATTCT TATGTCGGCC 
ACTATTGATA CAACGAAATT TTCCAAATAT TTTGGCATCT GTCCCGTATT GGAAGTACCC 
GGAAGAGCAT TTCCCGTGCA GCAATTTTTC TTGGAGGACA TTATTCAGAT GACAGACTTT 
GTGCCTTCGG CGGAGTCGCG CCGAAAGCGC AAGGAAGTGG AGGACGAGGA GCAATTGCTT 
TCCGAGGACA AGGACGAGGC GGAAATCAAC TATAACAAGG TGTGCGAGGA CAAGTATTCA 
CAAAAGACTC GAAATGCTAT GGCCATGCTA TCCGAATCGG ACGTTAGCTT TGAGCTGCTG 
GAGGCATTAT TGATGCATAT TAAGTCAAAA AATATTCCCG GTGCTATCTT AGTATTCCTG 
CCCGGTTGGA ACCTGATCTT TGCGCTTATG AAGTTTCTGC AAAATACAAA CATTTTTGGT 
GATACATCCC AATACCAAAT TTTGCCGTGC CACTCGCAGA TTCCACGAGA CGAGCAACGC 
AAGGTTTTCG AGCCTGTTCC AGAAGGCGTA ACCAAGATTA TACTTTCTAC CAACATTGCA 
GAGACTTCTA TAACTATTGA CGACATTGTT TTCGTAATTG ATATATGTAA GGCGCGCATG 
AAGCTCTTTA CTTCACATAA CAACCTAACC AGCTACGCCA CCGTTTGGGC AAGCAAAACC 
AATTTGGAGC AGCGCAAGGG CCGAGCTGGA CGTGTCCGAC CAGGCTTCTG CTTCACACTC 
TGTTCGCGTG CCCGCTTCCA AGCGCTAGAG GACAATCTTA CGCCGGAGAT GTTCCGCACA 
CCGTTGCACG AGATGGCGTT GACCATTAAA TTACTCAGAC TGGGCTCCAT TCACCACTTT 
TTGTCAAAGG CATTGGAGCC ACCTCCGGTA GACGCAGTAA TCGAAGCTGA GGTGTTGCTT 
CGCGAGATGC GCTGCCTTGA CGCTAACGAT GAACTTACTC CATTGGGGCG CCTGCTGGCA 
CGCCTCCCAA TTGAACCAAG ACTTGGAAAG ATGATGGTCC TCGGCGCAGT ATTTGGCTGC 
GCCGATCTAA TGGCCATTAT GGCTTCGTAC TCTAGCACCT TCTCAGAGGT GTTTAGTCTT 
GATATAGGTC AACGTCGCCT GGCAAATCAT CAGAAAGCGT TGAGTGGTAC CAAATGTTCC 
GACCACGTGG CAATGATTGT AGCTTCACAA ATGTGGCGGC GCGAGAAACA ACGCGGTGAG 
CATATGGAAG CTCGGTTCTG TGACTGGAAA GGACTGCAAA TGAGTACTAT GAACGTTATC 
TGGGATGCCA AGCAGCAGTT ACTGGATCTC CTTCAGCAGG CAGGATTCCC AGAAGAGTGT 
ATGATATCGC ACGAGGTAGA CGAAAGGATT GATGGGGATG ACCCCGTGCT GGATGTGTCC 
CTGGCGCTGC TGTGCTTGGG TCTCTACCCC AACATTTGCG TGCACAAGGA GAAGCGCAAA 
GTGCTGACTA CAGAGTCTAA AGCAGCGTTA CTGCACAAAA CCTCGGTGAA TTGCAGCAAC 
TTGGCCGTCA CATTTCCGTA TCCGTTCTTC GTTTTCGGCG AGAAGATTCG CACGCGAGCT 
GTTTCCTGCA AGCAACTATC TATGGTCTCG CCCTTGCAGG TGATATTGTT TGGATCGCGC 
AAGATTGACT TGGCCGCCAA CAATATAGTG CGCGTTGACA ACTGGCTTAA CTTCGACATA 
GAGCCGGAAC TTGCGGCCAA GATTGGTGCC CTTAAGCCGG CCCTGGAGGA TCTTATCACG 
GTGGCGTGCG ACAACCCTTC CGACATACTT CGCCTTGAGG AGCCCTATGC ACAGCTGGTC 
AAAGTGGTCA AAGACCTTTG CGTTAAGAGC GCCGGAGACT TCGGACTGCA GCGTGAATCA 
GGAATTCTGC CGCACCAATC ACGTCAGTTC AGTGACGGCG GAGGACCGCC CAAACGAGGT 
CGCTTTGAGA CAGGACGCTT TACTAACAGC AGCTTTGGTC GCCGTGGAAA CGGCAGAACT 
TTTGGAGGAG GATATGGAAA TAATGGAGGA GGATATGGAA ATAATGGAGG AGGATATGGA 
AATATTGGAG GAGGATATGG AAATAATGCA GGAGGTTATG GAAATAATGG TGGATATGGA 
AATAATGGCG GAGGTTATAG AAATAATGGT GGAGGATATG GAAACAATGG CGGAGGATAT 
GGGAACAAGA GAGGTGGATT TGGAGACTCA TTTGAAAGCA ATAGAGGATC AGGCGGCGGC 
TTTAGAAATG GCGACCAAGG CGGCCGCTGG GGTAACTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476641.1
CDS	85..3966
Translation	MDIKSFLYQFCAKSQIEPKFDIRQTGPKNRQRFLCEVRVEPNTYIGVGNSTNKKDAEKNACRDFVNYLVRVGKLNTNDVPADAGASGGGPRTGLEGAGMAGGSGQQKRVFDGQSGPQDLGEAYRPLNHDGGDGGNRYSVIDRIQEQRDMNEAEAFDVNAAIHGNWTIENAKERLNIYKQTNNIRDDYKYTPVGPEHARSFLAELSIYVPALNRTVTARESGSNKKSASKSCALSLVRQLFHLNVIEPFSGTLKKKKDEQLKPYPVKLSPNLINKIDEVIKGLDLPVVNPRNIKIELDGPPIPLIVNLSRIDSSQQDGEKRQESSVIPWAPPQANWNTWHACNIDEGELATTSIDDLSMDYERSLRDRRQNDNEYRQFLEFREKLPIAAMRSEILTAINDNPVVIIRGNTGCGKTTQIAQYILDDYICSGQGGYANIYVTQPRRISAISVAERVARERCEQLGDTVGYSVRFESVFPRPYGAILFCTVGVLLRKLEAGLRGVSHIIVDEIHERDVNSDFLLVILRDMVDTYPDLHVILMSATIDTTKFSKYFGICPVLEVPGRAFPVQQFFLEDIIQMTDFVPSAESRRKRKEVEDEEQLLSEDKDEAEINYNKVCEDKYSQKTRNAMAMLSESDVSFELLEALLMHIKSKNIPGAILVFLPGWNLIFALMKFLQNTNIFGDTSQYQILPCHSQIPRDEQRKVFEPVPEGVTKIILSTNIAETSITIDDIVFVIDICKARMKLFTSHNNLTSYATVWASKTNLEQRKGRAGRVRPGFCFTLCSRARFQALEDNLTPEMFRTPLHEMALTIKLLRLGSIHHFLSKALEPPPVDAVIEAEVLLREMRCLDANDELTPLGRLLARLPIEPRLGKMMVLGAVFGCADLMAIMASYSSTFSEVFSLDIGQRRLANHQKALSGTKCSDHVAMIVASQMWRREKQRGEHMEARFCDWKGLQMSTMNVIWDAKQQLLDLLQQAGFPEECMISHEVDERIDGDDPVLDVSLALLCLGLYPNICVHKEKRKVLTTESKAALLHKTSVNCSNLAVTFPYPFFVFGEKIRTRAVSCKQLSMVSPLQVILFGSRKIDLAANNIVRVDNWLNFDIEPELAAKIGALKPALEDLITVACDNPSDILRLEEPYAQLVKVVKDLCVKSAGDFGLQRESGILPHQSRQFSDGGGPPKRGRFETGRFTNSSFGRRGNGRTFGGGYGNNGGGYGNNGGGYGNIGGGYGNNAGGYGNNGGYGNNGGGYRNNGGGYGNNGGGYGNKRGGFGDSFESNRGSGGGFRNGDQGGRWGNF

Target ORF information:

RefSeq Version	NM_057293.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster maleless (mle), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057293.4

ORF Insert Sequence:

ATGGATATAA AATCTTTTTT GTACCAATTT TGTGCTAAGT CGCAAATAGA ACCAAAGTTT 
GATATTCGCC AAACGGGTCC CAAAAATCGC CAGCGGTTCC TTTGCGAGGT TCGTGTAGAG 
CCAAATACCT ATATTGGTGT GGGTAATTCC ACTAACAAAA AGGATGCCGA GAAAAATGCG 
TGCCGTGATT TTGTCAACTA CCTAGTGCGC GTGGGCAAAC TGAACACCAA TGATGTTCCA 
GCGGATGCTG GTGCTTCGGG GGGCGGACCT AGGACTGGTC TGGAGGGTGC GGGCATGGCA 
GGGGGCAGTG GACAGCAGAA GCGAGTGTTT GATGGTCAGT CGGGTCCACA GGATCTGGGT 
GAGGCCTATC GCCCTCTAAA CCACGATGGC GGTGACGGCG GTAACCGCTA CTCAGTAATT 
GATCGCATCC AGGAGCAGAG GGACATGAAC GAAGCGGAGG CTTTCGATGT CAACGCTGCC 
ATCCACGGTA ACTGGACCAT AGAGAATGCC AAAGAGCGAC TGAACATTTA CAAGCAGACG 
AACAACATTC GCGACGATTA CAAATATACA CCAGTGGGCC CGGAACACGC TAGGAGCTTT 
TTGGCCGAAT TGTCCATTTA TGTGCCGGCG CTCAATCGCA CGGTGACGGC CCGTGAGTCG 
GGTTCAAACA AGAAGTCTGC CAGCAAGTCA TGCGCCCTGT CCCTGGTGCG CCAACTTTTC 
CATTTAAATG TGATAGAGCC CTTTAGTGGA ACTCTGAAGA AAAAAAAGGA TGAGCAGCTG 
AAGCCATATC CGGTGAAGCT TTCGCCAAAT CTGATAAACA AAATTGATGA GGTGATTAAG 
GGTTTGGACC TTCCTGTCGT AAACCCGCGC AATATCAAAA TCGAACTGGA CGGTCCTCCG 
ATTCCTCTCA TTGTGAACTT AAGCCGTATA GATTCCTCGC AACAGGATGG CGAAAAACGT 
CAGGAAAGCT CCGTGATTCC GTGGGCTCCT CCTCAGGCAA ACTGGAATAC GTGGCATGCC 
TGCAACATCG ACGAAGGCGA ACTGGCAACC ACCTCTATTG ATGACCTTTC GATGGACTAC 
GAACGCTCGC TGCGTGATCG CCGCCAAAAC GACAACGAGT ACCGACAATT CCTCGAGTTC 
CGCGAAAAGC TGCCCATTGC AGCGATGCGG TCGGAAATTT TAACCGCAAT TAATGACAAT 
CCAGTGGTAA TCATTCGTGG AAACACAGGG TGCGGAAAGA CTACCCAGAT TGCCCAATAC 
ATTCTTGATG ACTACATCTG CTCCGGCCAG GGCGGGTACG CTAATATTTA TGTGACCCAG 
CCTCGTCGCA TTTCAGCCAT TTCTGTGGCC GAGCGCGTAG CTCGCGAGCG GTGCGAGCAA 
TTGGGCGACA CTGTAGGCTA CTCGGTGCGA TTTGAGTCTG TGTTTCCACG ACCCTACGGC 
GCAATCCTCT TTTGCACCGT GGGTGTGTTG CTGCGCAAGC TGGAGGCTGG CTTACGAGGA 
GTGTCGCATA TTATTGTGGA CGAAATCCAC GAGAGAGACG TGAACAGCGA CTTTTTGCTG 
GTCATCCTTC GCGACATGGT CGACACATAT CCGGACCTGC ATGTTATTCT TATGTCGGCC 
ACTATTGATA CAACGAAATT TTCCAAATAT TTTGGCATCT GTCCCGTATT GGAAGTACCC 
GGAAGAGCAT TTCCCGTGCA GCAATTTTTC TTGGAGGACA TTATTCAGAT GACAGACTTT 
GTGCCTTCGG CGGAGTCGCG CCGAAAGCGC AAGGAAGTGG AGGACGAGGA GCAATTGCTT 
TCCGAGGACA AGGACGAGGC GGAAATCAAC TATAACAAGG TGTGCGAGGA CAAGTATTCA 
CAAAAGACTC GAAATGCTAT GGCCATGCTA TCCGAATCGG ACGTTAGCTT TGAGCTGCTG 
GAGGCATTAT TGATGCATAT TAAGTCAAAA AATATTCCCG GTGCTATCTT AGTATTCCTG 
CCCGGTTGGA ACCTGATCTT TGCGCTTATG AAGTTTCTGC AAAATACAAA CATTTTTGGT 
GATACATCCC AATACCAAAT TTTGCCGTGC CACTCGCAGA TTCCACGAGA CGAGCAACGC 
AAGGTTTTCG AGCCTGTTCC AGAAGGCGTA ACCAAGATTA TACTTTCTAC CAACATTGCA 
GAGACTTCTA TAACTATTGA CGACATTGTT TTCGTAATTG ATATATGTAA GGCGCGCATG 
AAGCTCTTTA CTTCACATAA CAACCTAACC AGCTACGCCA CCGTTTGGGC AAGCAAAACC 
AATTTGGAGC AGCGCAAGGG CCGAGCTGGA CGTGTCCGAC CAGGCTTCTG CTTCACACTC 
TGTTCGCGTG CCCGCTTCCA AGCGCTAGAG GACAATCTTA CGCCGGAGAT GTTCCGCACA 
CCGTTGCACG AGATGGCGTT GACCATTAAA TTACTCAGAC TGGGCTCCAT TCACCACTTT 
TTGTCAAAGG CATTGGAGCC ACCTCCGGTA GACGCAGTAA TCGAAGCTGA GGTGTTGCTT 
CGCGAGATGC GCTGCCTTGA CGCTAACGAT GAACTTACTC CATTGGGGCG CCTGCTGGCA 
CGCCTCCCAA TTGAACCAAG ACTTGGAAAG ATGATGGTCC TCGGCGCAGT ATTTGGCTGC 
GCCGATCTAA TGGCCATTAT GGCTTCGTAC TCTAGCACCT TCTCAGAGGT GTTTAGTCTT 
GATATAGGTC AACGTCGCCT GGCAAATCAT CAGAAAGCGT TGAGTGGTAC CAAATGTTCC 
GACCACGTGG CAATGATTGT AGCTTCACAA ATGTGGCGGC GCGAGAAACA ACGCGGTGAG 
CATATGGAAG CTCGGTTCTG TGACTGGAAA GGACTGCAAA TGAGTACTAT GAACGTTATC 
TGGGATGCCA AGCAGCAGTT ACTGGATCTC CTTCAGCAGG CAGGATTCCC AGAAGAGTGT 
ATGATATCGC ACGAGGTAGA CGAAAGGATT GATGGGGATG ACCCCGTGCT GGATGTGTCC 
CTGGCGCTGC TGTGCTTGGG TCTCTACCCC AACATTTGCG TGCACAAGGA GAAGCGCAAA 
GTGCTGACTA CAGAGTCTAA AGCAGCGTTA CTGCACAAAA CCTCGGTGAA TTGCAGCAAC 
TTGGCCGTCA CATTTCCGTA TCCGTTCTTC GTTTTCGGCG AGAAGATTCG CACGCGAGCT 
GTTTCCTGCA AGCAACTATC TATGGTCTCG CCCTTGCAGG TGATATTGTT TGGATCGCGC 
AAGATTGACT TGGCCGCCAA CAATATAGTG CGCGTTGACA ACTGGCTTAA CTTCGACATA 
GAGCCGGAAC TTGCGGCCAA GATTGGTGCC CTTAAGCCGG CCCTGGAGGA TCTTATCACG 
GTGGCGTGCG ACAACCCTTC CGACATACTT CGCCTTGAGG AGCCCTATGC ACAGCTGGTC 
AAAGTGGTCA AAGACCTTTG CGTTAAGAGC GCCGGAGACT TCGGACTGCA GCGTGAATCA 
GGAATTCTGC CGCACCAATC ACGTCAGTTC AGTGACGGCG GAGGACCGCC CAAACGAGGT 
CGCTTTGAGA CAGGACGCTT TACTAACAGC AGCTTTGGTC GCCGTGGAAA CGGCAGAACT 
TTTGGAGGAG GATATGGAAA TAATGGAGGA GGATATGGAA ATAATGGAGG AGGATATGGA 
AATATTGGAG GAGGATATGG AAATAATGCA GGAGGTTATG GAAATAATGG TGGATATGGA 
AATAATGGCG GAGGTTATAG AAATAATGGT GGAGGATATG GAAACAATGG CGGAGGATAT 
GGGAACAAGA GAGGTGGATT TGGAGACTCA TTTGAAAGCA ATAGAGGATC AGGCGGCGGC 
TTTAGAAATG GCGACCAAGG CGGCCGCTGG GGTAACTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mle cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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