ap cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ap
Entrez Gene ID	35509
Full Name	apterous
Synonyms	AP,Ap,Apt,CG8376,Dmel\CG8376,LIM,S-2a,Xa,blt,neuro,trw
General protein information	Preferred Names apterous Names CG8376 gene product from transcript CG8376-RA APTEROUS CG8376-PA CG8376-PB CG8376-PC CG8376-PE ap-PA ap-PB ap-PC ap-PE apteroys neuromuscular no-wings tornwing xasta
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 41F-41F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_165445.4	NP_724428.1	apterous, isoform A
NM_078897.4	NP_523621.2	apterous, isoform B
NM_001169588.2	NP_001163059.1	apterous, isoform E
NM_001169587.2	NP_001163058.1	apterous, isoform C

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Lhx2	NP_001100041.1
Pan troglodytes (chimpanzee)	LHX2	XP_528427.2
Drosophila melanogaster (fruit fly)	ap	NP_001163058.1
Xenopus tropicalis (tropical clawed frog)	lhx2	XP_002938795.1
	LHX2	XP_001083598.2
Bos taurus (cattle)	LHX2	NP_001178104.1
Mus musculus (house mouse)	Lhx2	NP_034840.1
Danio rerio (zebrafish)	lhx2b	NP_001035099.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008980	XP_552873.2
Gallus gallus (chicken)	LHX2	NP_990220.1
Homo sapiens (human)	LHX2	NP_004780.3
Canis lupus familiaris (dog)	LHX2	XP_005625444.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nplp1 neuropeptide neurons in the developing Drosophila nerve cord belong to two sub-classes; Tv1 and dAp neurons, generated by two distinct progenitors. Nplp1 neurons are specified by spatial cues
Title: Neuronal cell fate specification by the molecular convergence of different spatio-temporal cues on a common initiator terminal selector gene.

Ap role in the neuronal cell fate specification
Title: Neuronal Cell Fate Specification by the Convergence of Different Spatiotemporal Cues on a Common Terminal Selector Cascade.

our results indicate that ap is regulated in time and space by a three-step mechanism that generates a lineage compartment by integrating input from separatecis-regulatory modules for the initiation, refinement and maintenance of its expression
Title: Establishment of a Developmental Compartment Requires Interactions between Three Synergistic Cis-regulatory Modules.

The AP and NAT activities in ap56f females were significantly lower than in Canton S females in the norm
Title: [Effect of the apterous56f mutation on N-acetyltransferase and alkaline phosphatase activities in Drosophila melanogaster females].

A new instance of transvection at the Drosophila apterous (ap) locus, is reported.
Title: Enhancer blocking and transvection at the Drosophila apterous locus.

The following ap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa08998	NM_001169588.2 Latest version!	Drosophila melanogaster apterous (ap), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08998	NM_078897.4 Latest version!	Drosophila melanogaster apterous (ap), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08999	NM_001169587.2 Latest version!	Drosophila melanogaster apterous (ap), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa12403	NM_165445.4 Latest version!	Drosophila melanogaster apterous (ap), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ap ( NM_078897.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523621.2 Drosophila melanogaster apterous (ap), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08998
Clone ID Related Accession (Same CDS sequence)	NM_078897.4 , NM_001169588.2
Accession Version	NM_078897.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	apterous, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_078897.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCGCCA GAAATCTTGT TTTTCACGTC AACTGCTTCT GCTGCACTGT CTGCCACACG 
CCACTGACAA AGGGAGACCA GTACGGCATC ATCGACGCCC TCATCTACTG CAGGACCCAC 
TACAGCATAG CCAGGGAGGG GGATACCGCC TCATCCAGTA TGAGCGCCAC CTACCCGTAC 
AGCGCCCAGT TCGGCTCACC CCACAACGAC TCCTCGAGCC CGCACTCGGA CCCTAGTCGG 
AGCATTGTTC CTACGGGCAT CTTTGTGCCC GCGTCCCACG TAATCAACGG ACTGCCGCAG 
CCAGCTCGTC AAAAGGGCAG GCCCCGCAAG CGCAAGCCCA AGGACATCGA GGCGTTCACC 
GCTAACATAG ATCTCAACAC TGAGTACGTG GACTTTGGCC GAGGCTCGCA CCTAAGCTCC 
TCGTCGCGCA CCAAACGAAT GCGAACCTCG TTTAAGCATC ACCAGCTGCG TACCATGAAG 
TCCTACTTTG CCATTAATCA TAACCCTGAT GCAAAGGATC TAAAACAATT GTCACAGAAA 
ACTGGTTTAC CAAAGAGGGT CCTACAGGTC TGGTTTCAAA ATGCAAGGGC CAAATGGCGC 
CGCATGATGA TGAAGCAGGA TGGCAGCGGC CTGCTGGAGA AGGGCGAAGG CGCTCTGGAC 
CTCGACAGCA TCTCCGTGCA CAGTCCTACG TCGTTTATAT TGGGAGGACC GAACAGCACA 
CCGCCACTTA ACTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523621.2
CDS	196..936
Translation	MRARNLVFHVNCFCCTVCHTPLTKGDQYGIIDALIYCRTHYSIAREGDTASSSMSATYPYSAQFGSPHNDSSSPHSDPSRSIVPTGIFVPASHVINGLPQPARQKGRPRKRKPKDIEAFTANIDLNTEYVDFGRGSHLSSSSRTKRMRTSFKHHQLRTMKSYFAINHNPDAKDLKQLSQKTGLPKRVLQVWFQNARAKWRRMMMKQDGSGLLEKGEGALDLDSISVHSPTSFILGGPNSTPPLNLD

Target ORF information:

RefSeq Version	NM_078897.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster apterous (ap), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078897.4

ORF Insert Sequence:

ATGCGCGCCA GAAATCTTGT TTTTCACGTC AACTGCTTCT GCTGCACTGT CTGCCACACG 
CCACTGACAA AGGGAGACCA GTACGGCATC ATCGACGCCC TCATCTACTG CAGGACCCAC 
TACAGCATAG CCAGGGAGGG GGATACCGCC TCATCCAGTA TGAGCGCCAC CTACCCGTAC 
AGCGCCCAGT TCGGCTCACC CCACAACGAC TCCTCGAGCC CGCACTCGGA CCCTAGTCGG 
AGCATTGTTC CTACGGGCAT CTTTGTGCCC GCGTCCCACG TAATCAACGG ACTGCCGCAG 
CCAGCTCGTC AAAAGGGCAG GCCCCGCAAG CGCAAGCCCA AGGACATCGA GGCGTTCACC 
GCTAACATAG ATCTCAACAC TGAGTACGTG GACTTTGGCC GAGGCTCGCA CCTAAGCTCC 
TCGTCGCGCA CCAAACGAAT GCGAACCTCG TTTAAGCATC ACCAGCTGCG TACCATGAAG 
TCCTACTTTG CCATTAATCA TAACCCTGAT GCAAAGGATC TAAAACAATT GTCACAGAAA 
ACTGGTTTAC CAAAGAGGGT CCTACAGGTC TGGTTTCAAA ATGCAAGGGC CAAATGGCGC 
CGCATGATGA TGAAGCAGGA TGGCAGCGGC CTGCTGGAGA AGGGCGAAGG CGCTCTGGAC 
CTCGACAGCA TCTCCGTGCA CAGTCCTACG TCGTTTATAT TGGGAGGACC GAACAGCACA 
CCGCCACTTA ACTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ap ( NM_001169588.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163059.1 Drosophila melanogaster apterous (ap), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa08998
Clone ID Related Accession (Same CDS sequence)	NM_078897.4 , NM_001169588.2
Accession Version	NM_001169588.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	apterous, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169588.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCGCCA GAAATCTTGT TTTTCACGTC AACTGCTTCT GCTGCACTGT CTGCCACACG 
CCACTGACAA AGGGAGACCA GTACGGCATC ATCGACGCCC TCATCTACTG CAGGACCCAC 
TACAGCATAG CCAGGGAGGG GGATACCGCC TCATCCAGTA TGAGCGCCAC CTACCCGTAC 
AGCGCCCAGT TCGGCTCACC CCACAACGAC TCCTCGAGCC CGCACTCGGA CCCTAGTCGG 
AGCATTGTTC CTACGGGCAT CTTTGTGCCC GCGTCCCACG TAATCAACGG ACTGCCGCAG 
CCAGCTCGTC AAAAGGGCAG GCCCCGCAAG CGCAAGCCCA AGGACATCGA GGCGTTCACC 
GCTAACATAG ATCTCAACAC TGAGTACGTG GACTTTGGCC GAGGCTCGCA CCTAAGCTCC 
TCGTCGCGCA CCAAACGAAT GCGAACCTCG TTTAAGCATC ACCAGCTGCG TACCATGAAG 
TCCTACTTTG CCATTAATCA TAACCCTGAT GCAAAGGATC TAAAACAATT GTCACAGAAA 
ACTGGTTTAC CAAAGAGGGT CCTACAGGTC TGGTTTCAAA ATGCAAGGGC CAAATGGCGC 
CGCATGATGA TGAAGCAGGA TGGCAGCGGC CTGCTGGAGA AGGGCGAAGG CGCTCTGGAC 
CTCGACAGCA TCTCCGTGCA CAGTCCTACG TCGTTTATAT TGGGAGGACC GAACAGCACA 
CCGCCACTTA ACTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163059.1
CDS	700..1440
Translation	MRARNLVFHVNCFCCTVCHTPLTKGDQYGIIDALIYCRTHYSIAREGDTASSSMSATYPYSAQFGSPHNDSSSPHSDPSRSIVPTGIFVPASHVINGLPQPARQKGRPRKRKPKDIEAFTANIDLNTEYVDFGRGSHLSSSSRTKRMRTSFKHHQLRTMKSYFAINHNPDAKDLKQLSQKTGLPKRVLQVWFQNARAKWRRMMMKQDGSGLLEKGEGALDLDSISVHSPTSFILGGPNSTPPLNLD

Target ORF information:

RefSeq Version	NM_001169588.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster apterous (ap), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169588.2

ORF Insert Sequence:

ATGCGCGCCA GAAATCTTGT TTTTCACGTC AACTGCTTCT GCTGCACTGT CTGCCACACG 
CCACTGACAA AGGGAGACCA GTACGGCATC ATCGACGCCC TCATCTACTG CAGGACCCAC 
TACAGCATAG CCAGGGAGGG GGATACCGCC TCATCCAGTA TGAGCGCCAC CTACCCGTAC 
AGCGCCCAGT TCGGCTCACC CCACAACGAC TCCTCGAGCC CGCACTCGGA CCCTAGTCGG 
AGCATTGTTC CTACGGGCAT CTTTGTGCCC GCGTCCCACG TAATCAACGG ACTGCCGCAG 
CCAGCTCGTC AAAAGGGCAG GCCCCGCAAG CGCAAGCCCA AGGACATCGA GGCGTTCACC 
GCTAACATAG ATCTCAACAC TGAGTACGTG GACTTTGGCC GAGGCTCGCA CCTAAGCTCC 
TCGTCGCGCA CCAAACGAAT GCGAACCTCG TTTAAGCATC ACCAGCTGCG TACCATGAAG 
TCCTACTTTG CCATTAATCA TAACCCTGAT GCAAAGGATC TAAAACAATT GTCACAGAAA 
ACTGGTTTAC CAAAGAGGGT CCTACAGGTC TGGTTTCAAA ATGCAAGGGC CAAATGGCGC 
CGCATGATGA TGAAGCAGGA TGGCAGCGGC CTGCTGGAGA AGGGCGAAGG CGCTCTGGAC 
CTCGACAGCA TCTCCGTGCA CAGTCCTACG TCGTTTATAT TGGGAGGACC GAACAGCACA 
CCGCCACTTA ACTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ap ( NM_001169587.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163058.1 Drosophila melanogaster apterous (ap), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa08999
Clone ID Related Accession (Same CDS sequence)	NM_001169587.2
Accession Version	NM_001169587.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	apterous, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169587.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGTCT GCACCGAGGA GCGCCCTGTG ATGCATTGGC AGAGCGCAAG ATTTCTTGGG 
CCCGGCGCAA GGGAGAAAAG TCCAACACCA CCTGTAGCAC ATCAAGGGAG CAATCAATGT 
GGCAGTGCTG CAGGTGCAAA TAACAATCAC CCATTGTTCC GCGCGTGCTC CTCATCCTCG 
TGTCCAGATA TTTGTGATCA TAGTACAAAG CCATTTGGCA ACGCTTACGG CACCGAGTCA 
TTTAGAAGCT ACGAAACAGC CGATCGTGCT ACCTTTGAGG ACTCAGCCGC CAAATTCTCC 
ATCAGCCGCA GTCGAACAGA CTGCACGGAG GTCAGCGACG AGACGACGTC GGGCATATCA 
TTCAAGACCG AACCCTTCGG ACCGCCCAGC AGTCCCGAGT CCACAAGCGA TAGCAAAATA 
ACGCGCAACC TCGACGACTG CTCCGGCTGC GGACGTCAGA TACAGGATCG CTTCTACCTC 
TCCGCTGTGG AAAAACGGTG GCATGCAAGT TGCCTACAGT GCTACGCCTG TCGGCAGCCG 
CTGGAACGGG AATCCTCATG CTACTCACGT GACGGCAACA TTTATTGCAA AAACGATTAT 
TATAGTTTTT TTGGTACTCG CCGATGCTCG CGCTGCCTGG CCTCCATCAG CTCCAACGAG 
CTGGTCATGC GCGCCAGAAA TCTTGTTTTT CACGTCAACT GCTTCTGCTG CACTGTCTGC 
CACACGCCAC TGACAAAGGG AGACCAGTAC GGCATCATCG ACGCCCTCAT CTACTGCAGG 
ACCCACTACA GCATAGCCAG GGAGGGGGAT ACCGCCTCAT CCAGTATGAG CGCCACCTAC 
CCGTACAGCG CCCAGTTCGG CTCACCCCAC AACGACTCCT CGAGCCCGCA CTCGGACCCT 
AGTCGGAGCA TTGTTCCTAC GGGCATCTTT GTGCCCGCGT CCCACGTAAT CAACGGACTG 
CCGCAGCCAG CTCGTCAAAA GGGCAGGCCC CGCAAGCGCA AGCCCAAGGA CATCGAGGCG 
TTCACCGCTA ACATAGATCT CAACACTGAG TACGTGGACT TTGGCCGAGG CTCGCACCTA 
AGCTCCTCGT CGCGCACCAA ACGAATGCGA ACCTCGTTTA AGCATCACCA GCTGCGTACC 
ATGAAGTCCT ACTTTGCCAT TAATCATAAC CCTGATGCAA AGGATCTAAA ACAATTGTCA 
CAGAAAACTG GTTTACCAAA GAGGGTCCTA CAGGTCTGGT TTCAAAATGC AAGGGCCAAA 
TGGCGCCGCA TGATGATGAA GCAGGATGGC AGCGGCCTGC TGGAGAAGGG CGAAGGCGCT 
CTGGACCTCG ACAGCATCTC CGTGCACAGT CCTACGTCGT TTATATTGGG AGGACCGAAC 
AGCACACCGC CACTTAACTT GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163058.1
CDS	974..2380
Translation	MGVCTEERPVMHWQSARFLGPGAREKSPTPPVAHQGSNQCGSAAGANNNHPLFRACSSSSCPDICDHSTKPFGNAYGTESFRSYETADRATFEDSAAKFSISRSRTDCTEVSDETTSGISFKTEPFGPPSSPESTSDSKITRNLDDCSGCGRQIQDRFYLSAVEKRWHASCLQCYACRQPLERESSCYSRDGNIYCKNDYYSFFGTRRCSRCLASISSNELVMRARNLVFHVNCFCCTVCHTPLTKGDQYGIIDALIYCRTHYSIAREGDTASSSMSATYPYSAQFGSPHNDSSSPHSDPSRSIVPTGIFVPASHVINGLPQPARQKGRPRKRKPKDIEAFTANIDLNTEYVDFGRGSHLSSSSRTKRMRTSFKHHQLRTMKSYFAINHNPDAKDLKQLSQKTGLPKRVLQVWFQNARAKWRRMMMKQDGSGLLEKGEGALDLDSISVHSPTSFILGGPNSTPPLNLD

Target ORF information:

RefSeq Version	NM_001169587.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster apterous (ap), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169587.2

ORF Insert Sequence:

ATGGGCGTCT GCACCGAGGA GCGCCCTGTG ATGCATTGGC AGAGCGCAAG ATTTCTTGGG 
CCCGGCGCAA GGGAGAAAAG TCCAACACCA CCTGTAGCAC ATCAAGGGAG CAATCAATGT 
GGCAGTGCTG CAGGTGCAAA TAACAATCAC CCATTGTTCC GCGCGTGCTC CTCATCCTCG 
TGTCCAGATA TTTGTGATCA TAGTACAAAG CCATTTGGCA ACGCTTACGG CACCGAGTCA 
TTTAGAAGCT ACGAAACAGC CGATCGTGCT ACCTTTGAGG ACTCAGCCGC CAAATTCTCC 
ATCAGCCGCA GTCGAACAGA CTGCACGGAG GTCAGCGACG AGACGACGTC GGGCATATCA 
TTCAAGACCG AACCCTTCGG ACCGCCCAGC AGTCCCGAGT CCACAAGCGA TAGCAAAATA 
ACGCGCAACC TCGACGACTG CTCCGGCTGC GGACGTCAGA TACAGGATCG CTTCTACCTC 
TCCGCTGTGG AAAAACGGTG GCATGCAAGT TGCCTACAGT GCTACGCCTG TCGGCAGCCG 
CTGGAACGGG AATCCTCATG CTACTCACGT GACGGCAACA TTTATTGCAA AAACGATTAT 
TATAGTTTTT TTGGTACTCG CCGATGCTCG CGCTGCCTGG CCTCCATCAG CTCCAACGAG 
CTGGTCATGC GCGCCAGAAA TCTTGTTTTT CACGTCAACT GCTTCTGCTG CACTGTCTGC 
CACACGCCAC TGACAAAGGG AGACCAGTAC GGCATCATCG ACGCCCTCAT CTACTGCAGG 
ACCCACTACA GCATAGCCAG GGAGGGGGAT ACCGCCTCAT CCAGTATGAG CGCCACCTAC 
CCGTACAGCG CCCAGTTCGG CTCACCCCAC AACGACTCCT CGAGCCCGCA CTCGGACCCT 
AGTCGGAGCA TTGTTCCTAC GGGCATCTTT GTGCCCGCGT CCCACGTAAT CAACGGACTG 
CCGCAGCCAG CTCGTCAAAA GGGCAGGCCC CGCAAGCGCA AGCCCAAGGA CATCGAGGCG 
TTCACCGCTA ACATAGATCT CAACACTGAG TACGTGGACT TTGGCCGAGG CTCGCACCTA 
AGCTCCTCGT CGCGCACCAA ACGAATGCGA ACCTCGTTTA AGCATCACCA GCTGCGTACC 
ATGAAGTCCT ACTTTGCCAT TAATCATAAC CCTGATGCAA AGGATCTAAA ACAATTGTCA 
CAGAAAACTG GTTTACCAAA GAGGGTCCTA CAGGTCTGGT TTCAAAATGC AAGGGCCAAA 
TGGCGCCGCA TGATGATGAA GCAGGATGGC AGCGGCCTGC TGGAGAAGGG CGAAGGCGCT 
CTGGACCTCG ACAGCATCTC CGTGCACAGT CCTACGTCGT TTATATTGGG AGGACCGAAC 
AGCACACCGC CACTTAACTT GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ap ( NM_165445.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724428.1 Drosophila melanogaster apterous (ap), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12403
Clone ID Related Accession (Same CDS sequence)	NM_165445.4
Accession Version	NM_165445.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	apterous, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165445.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGTCT GCACCGAGGA GCGCCCTGTG ATGCATTGGC AGCAGAGCGC AAGATTTCTT 
GGGCCCGGCG CAAGGGAGAA AAGTCCAACA CCACCTGTAG CACATCAAGG GAGCAATCAA 
TGTGGCAGTG CTGCAGGTGC AAATAACAAT CACCCATTGT TCCGCGCGTG CTCCTCATCC 
TCGTGTCCAG ATATTTGTGA TCATAGTACA AAGCCATTTG GCAACGCTTA CGGCACCGAG 
TCATTTAGAA GCTACGAAAC AGCCGATCGT GCTACCTTTG AGGACTCAGC CGCCAAATTC 
TCCATCAGCC GCAGTCGAAC AGACTGCACG GAGGTCAGCG ACGAGACGAC GTCGGGCATA 
TCATTCAAGA CCGAACCCTT CGGACCGCCC AGCAGTCCCG AGTCCACAAG CGATAGCAAA 
ATAACGCGCA ACCTCGACGA CTGCTCCGGC TGCGGACGTC AGATACAGGA TCGCTTCTAC 
CTCTCCGCTG TGGAAAAACG GTGGCATGCA AGTTGCCTAC AGTGCTACGC CTGTCGGCAG 
CCGCTGGAAC GGGAATCCTC ATGCTACTCA CGTGACGGCA ACATTTATTG CAAAAACGAT 
TATTATAGTT TTTTTGGTAC TCGCCGATGC TCGCGCTGCC TGGCCTCCAT CAGCTCCAAC 
GAGCTGGTCA TGCGCGCCAG AAATCTTGTT TTTCACGTCA ACTGCTTCTG CTGCACTGTC 
TGCCACACGC CACTGACAAA GGGAGACCAG TACGGCATCA TCGACGCCCT CATCTACTGC 
AGGACCCACT ACAGCATAGC CAGGGAGGGG GATACCGCCT CATCCAGTAT GAGCGCCACC 
TACCCGTACA GCGCCCAGTT CGGCTCACCC CACAACGACT CCTCGAGCCC GCACTCGGAC 
CCTAGTCGGA GCATTGTTCC TACGGGCATC TTTGTGCCCG CGTCCCACGT AATCAACGGA 
CTGCCGCAGC CAGCTCGTCA AAAGGGCAGG CCCCGCAAGC GCAAGCCCAA GGACATCGAG 
GCGTTCACCG CTAACATAGA TCTCAACACT GAGTACGTGG ACTTTGGCCG AGGCTCGCAC 
CTAAGCTCCT CGTCGCGCAC CAAACGAATG CGAACCTCGT TTAAGCATCA CCAGCTGCGT 
ACCATGAAGT CCTACTTTGC CATTAATCAT AACCCTGATG CAAAGGATCT AAAACAATTG 
TCACAGAAAA CTGGTTTACC AAAGAGGGTC CTACAGGTCT GGTTTCAAAA TGCAAGGGCC 
AAATGGCGCC GCATGATGAT GAAGCAGGAT GGCAGCGGCC TGCTGGAGAA GGGCGAAGGC 
GCTCTGGACC TCGACAGCAT CTCCGTGCAC AGTCCTACGT CGTTTATATT GGGAGGACCG 
AACAGCACAC CGCCACTTAA CTTGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724428.1
CDS	974..2383
Translation	MGVCTEERPVMHWQQSARFLGPGAREKSPTPPVAHQGSNQCGSAAGANNNHPLFRACSSSSCPDICDHSTKPFGNAYGTESFRSYETADRATFEDSAAKFSISRSRTDCTEVSDETTSGISFKTEPFGPPSSPESTSDSKITRNLDDCSGCGRQIQDRFYLSAVEKRWHASCLQCYACRQPLERESSCYSRDGNIYCKNDYYSFFGTRRCSRCLASISSNELVMRARNLVFHVNCFCCTVCHTPLTKGDQYGIIDALIYCRTHYSIAREGDTASSSMSATYPYSAQFGSPHNDSSSPHSDPSRSIVPTGIFVPASHVINGLPQPARQKGRPRKRKPKDIEAFTANIDLNTEYVDFGRGSHLSSSSRTKRMRTSFKHHQLRTMKSYFAINHNPDAKDLKQLSQKTGLPKRVLQVWFQNARAKWRRMMMKQDGSGLLEKGEGALDLDSISVHSPTSFILGGPNSTPPLNLD

Target ORF information:

RefSeq Version	NM_165445.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster apterous (ap), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165445.4

ORF Insert Sequence:

ATGGGCGTCT GCACCGAGGA GCGCCCTGTG ATGCATTGGC AGCAGAGCGC AAGATTTCTT 
GGGCCCGGCG CAAGGGAGAA AAGTCCAACA CCACCTGTAG CACATCAAGG GAGCAATCAA 
TGTGGCAGTG CTGCAGGTGC AAATAACAAT CACCCATTGT TCCGCGCGTG CTCCTCATCC 
TCGTGTCCAG ATATTTGTGA TCATAGTACA AAGCCATTTG GCAACGCTTA CGGCACCGAG 
TCATTTAGAA GCTACGAAAC AGCCGATCGT GCTACCTTTG AGGACTCAGC CGCCAAATTC 
TCCATCAGCC GCAGTCGAAC AGACTGCACG GAGGTCAGCG ACGAGACGAC GTCGGGCATA 
TCATTCAAGA CCGAACCCTT CGGACCGCCC AGCAGTCCCG AGTCCACAAG CGATAGCAAA 
ATAACGCGCA ACCTCGACGA CTGCTCCGGC TGCGGACGTC AGATACAGGA TCGCTTCTAC 
CTCTCCGCTG TGGAAAAACG GTGGCATGCA AGTTGCCTAC AGTGCTACGC CTGTCGGCAG 
CCGCTGGAAC GGGAATCCTC ATGCTACTCA CGTGACGGCA ACATTTATTG CAAAAACGAT 
TATTATAGTT TTTTTGGTAC TCGCCGATGC TCGCGCTGCC TGGCCTCCAT CAGCTCCAAC 
GAGCTGGTCA TGCGCGCCAG AAATCTTGTT TTTCACGTCA ACTGCTTCTG CTGCACTGTC 
TGCCACACGC CACTGACAAA GGGAGACCAG TACGGCATCA TCGACGCCCT CATCTACTGC 
AGGACCCACT ACAGCATAGC CAGGGAGGGG GATACCGCCT CATCCAGTAT GAGCGCCACC 
TACCCGTACA GCGCCCAGTT CGGCTCACCC CACAACGACT CCTCGAGCCC GCACTCGGAC 
CCTAGTCGGA GCATTGTTCC TACGGGCATC TTTGTGCCCG CGTCCCACGT AATCAACGGA 
CTGCCGCAGC CAGCTCGTCA AAAGGGCAGG CCCCGCAAGC GCAAGCCCAA GGACATCGAG 
GCGTTCACCG CTAACATAGA TCTCAACACT GAGTACGTGG ACTTTGGCCG AGGCTCGCAC 
CTAAGCTCCT CGTCGCGCAC CAAACGAATG CGAACCTCGT TTAAGCATCA CCAGCTGCGT 
ACCATGAAGT CCTACTTTGC CATTAATCAT AACCCTGATG CAAAGGATCT AAAACAATTG 
TCACAGAAAA CTGGTTTACC AAAGAGGGTC CTACAGGTCT GGTTTCAAAA TGCAAGGGCC 
AAATGGCGCC GCATGATGAT GAAGCAGGAT GGCAGCGGCC TGCTGGAGAA GGGCGAAGGC 
GCTCTGGACC TCGACAGCAT CTCCGTGCAC AGTCCTACGT CGTTTATATT GGGAGGACCG 
AACAGCACAC CGCCACTTAA CTTGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ap cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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