Not3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Not3
Entrez Gene ID	35501
Full Name	CCR4-NOT transcription complex subunit 3
Synonyms	CG8426,CNOT3,Dmel\CG8426,NOT3,NOT3.5,NOT3/5,l(2)NC136,not3
General protein information	Preferred Names CCR4-NOT transcription complex subunit 3 Names CG8426 gene product from transcript CG8426-RB CG8426-PA CG8426-PB Not3-PA Not3-PB lethal (2) NC136 lethal(2)NC136
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 41F6-41F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273783.1	NP_001260712.1	CCR4-NOT transcription complex subunit 3, isoform B
NM_136332.3	NP_610176.1	CCR4-NOT transcription complex subunit 3, isoform A

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	ntl-3	NP_001076652.1
Drosophila melanogaster (fruit fly)	l(2)NC136	NP_001260712.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The crystal structure of the NED central region of Nanos bound to the NOT module reveals an unanticipated bipartite binding interface that contacts NOT1 and NOT3 and is distinct from the NOT1 interacting motif of vertebrate Nanos.
Title: Distinct modes of recruitment of the CCR4-NOT complex by Drosophila and vertebrate Nanos.

The following Not3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Not3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08997	NM_001273783.1 Latest version!	Drosophila melanogaster CCR4-NOT transcription complex subunit 3 (Not3), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08997	NM_136332.3 Latest version!	Drosophila melanogaster CCR4-NOT transcription complex subunit 3 (Not3), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Not3 ( NM_001273783.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260712.1 Drosophila melanogaster CCR4-NOT transcription complex subunit 3 (Not3), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08997
Clone ID Related Accession (Same CDS sequence)	NM_001273783.1 , NM_136332.3
Accession Version	NM_001273783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	CCR4-NOT transcription complex subunit 3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCGA CGAGAAAATT GCAAGGCGAA ATTGATCGAT GTTTAAAAAA GGTAGCAGAG 
GGCGTTGAAA CGTTTGAGGA TATTTGGAAA AAAGTACACA ATGCGACAAA CACCAATCAG 
AAGCAAAAAC ATCTGCAGGA GAAGTATGAG GCAGACCTCA AAAAGGAAAT CAAGAAATTG 
CAACGACTTC GCGACCAAAT TAAATCATGG ATTGCATCAG CTGAAATTAA GGATAAGAGC 
TCCCTTCTTG AGAATCGACG TCTCATTGAA ACACAAATGG AACGCTTTAA GGTGGTTGAG 
CGCGAGACGA AAACAAAAGC CTACTCCAAG GAAGGCTTGG GGGCTGCACA AAAGATGGAT 
CCTGCCCAGC GTATTAAAGA CGACGCACGC AATTGGTTAA CCAGCTCAAT AAGTTCCCTT 
CAAATACAAA TTGACCAGTA TGAGAGCGAG ATCGAATCGT TGTTGGCAGG TAAAAAGAAA 
CGTTTAGATC GAGACAAGCA GGAGCGAATG GATGACCTAC GAGGAAAGTT GGACCGCCAT 
AAGTTCCACA TAACAAAGCT GGAAACTCTG CTTAGGCTGC TTGACAATGA CGGCGTCGAA 
GCAGAGCAGG TAAACAAGAT CAAAGACGAT GTCGAATACT ACATTGATTC ATCGCAAGAG 
CCAGACTTTG AGGAAAATGA GTTCATATAC GATGATATAA TTGGTCTTGA TGAAGTGGAG 
CTAAGTGGCA CGGCTACCAC GGACAGCAAC AACAGCAACG AGACAAGCGG TTCTCCAAGC 
AGTGTCACAT CCGGAGGCAG TCCATCACAG TCCCCTGTCA CGGTTCAACA AATTTTAAAC 
ACTTCGTCGC AGGGGGCAGC TAGTAGTGGT AGCAGCGCAG CTAGCGCAGC ACTGTTTCAG 
CAGCAGCTGA CAGCGGCACA ATCGAATGGC AACAATGTGG GCTACGCGAG CGACACAAGT 
GCAGCTTCTT CGTCTGCTAC AACATCTACA GATCCTGCAG GCGGCACAGT TGCTGTAAAT 
TGTGTTGGAG GGCTAGCCGA TAAACGCAAC AAGAGCAGCG AAAGCAATGC CCTAAAATTG 
AAACCACAAC CTCATCAACT TATAAAACCT ACCCCTGTAC GAGCCACGGC CAAGCTGCCT 
CTGAGCAGTG ATACCCAAGT CAATAAGATT GTCAGTTCAA CTCCCAGCAA AAATCAGCAA 
CAGCTGCCTA CAGCAGCTTC AATCGTTGCA ACCTCCGCTA TGCAATCGCA AAGCAGTATC 
GGAAGTTGCA GCTCCACTGG AGGAACGGGA GCATCTCAAT CAGCTTCTAG TGGTAACAAT 
CCTGGTAACA ATCCTGCGGT TCAGCCAAAT GCTCCAACGC CTGGTCAAAG CGGCATTGCC 
GCTGCAGCAG CTTCGACTAA TGTTGTTTCG GCGACGATTG TGAGCAGCGC CAATGTTCAA 
GGCCAATCTG TGATCCAACC AACACCAACA ATTGCATTTG CTGCAGTAGC AAAACACAAT 
ACATCACTCC TGGAAAATGG TCCTGTTTTG CAACAGCAAC TAGCAGTAAC TCCTACTGTG 
GCAGCTATCG TAGGAGCTGG AACGCAGGCG CAGCAAAAAC ATGTGCCACC ATTATCCAAT 
CTTCAAACAA ATTCCCCTCA CATACAAAAT GGTCTTCCTG TCTCGGACAG TACTAATGAC 
AACAGCTGCA ATGTCGTCGA TACGATTTCT CTAAAAACAA TGGCACAGGA TGCTATTAAT 
CGATCTGCGA TCGATCCTAA TTCACTGAAT CAGCAGCAAA CAAGCAGCAT CGATCTGAGA 
CAGCCGCAGT CGCAGAAATC ACTTCTCCAA CATTTCAACT CTGAAACCAA CACGAATCAA 
CAGCAATTGA CATCGCAGCA GCAGCAACAG CTTCAGAATA ACTCCCTTGC TGCGACCACA 
GGTTCTAACA ACGGAACTTC AACTGGTAGT GGTCTCATGA ATGTAGCAAA CGCTACTGGT 
CAGCCTATCA GCGGCAATGC CAAAACGCAT GCGTGCCAAC CCCAACAGAT GGCCACGACG 
GAAGCGCACA TTCCCACACT GCTTGGTGTG ACACCACTTG GGCCAACACC ACTTCAAAAG 
GAGCACCAAA TGCAGTTCCA AATGATGGAA GCTGCCTACT ATCACCTGCC ACAGCCTATG 
GACACGGAAA AGCTACAAAC GTACTTTCAT CGCGCACCAG TGCTCACGCC ATCGCACTAT 
CCCCAGGCAC AGATGCCAAT ATATGATACA GTTGAGTTTT ATCAACGACT CTCAACGGAG 
ACCCTTTTTT TTGTGTTCTA CTATATGGAG GGCTCCAAGG CCCAGTATCT AGCGGCCAAG 
GCCTTAAAAA AGCAGAGCTG GCGTTTCCAC ACAAAGTATA TGATGTGGTT TCAAAGGCAC 
GAGGAGCCGA AGATTATCAA CGATGATTAC GAACAGGGTA CGTACATCTA TTTTGATTAT 
GAAAAGTGGA GTCAGCGAAA GAAGGAGGGA TTCACCTTTG AATACAAGTA CTTAGAGGAC 
AAGGAGCTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260712.1
CDS	580..3114
Translation	MAATRKLQGEIDRCLKKVAEGVETFEDIWKKVHNATNTNQKQKHLQEKYEADLKKEIKKLQRLRDQIKSWIASAEIKDKSSLLENRRLIETQMERFKVVERETKTKAYSKEGLGAAQKMDPAQRIKDDARNWLTSSISSLQIQIDQYESEIESLLAGKKKRLDRDKQERMDDLRGKLDRHKFHITKLETLLRLLDNDGVEAEQVNKIKDDVEYYIDSSQEPDFEENEFIYDDIIGLDEVELSGTATTDSNNSNETSGSPSSVTSGGSPSQSPVTVQQILNTSSQGAASSGSSAASAALFQQQLTAAQSNGNNVGYASDTSAASSSATTSTDPAGGTVAVNCVGGLADKRNKSSESNALKLKPQPHQLIKPTPVRATAKLPLSSDTQVNKIVSSTPSKNQQQLPTAASIVATSAMQSQSSIGSCSSTGGTGASQSASSGNNPGNNPAVQPNAPTPGQSGIAAAAASTNVVSATIVSSANVQGQSVIQPTPTIAFAAVAKHNTSLLENGPVLQQQLAVTPTVAAIVGAGTQAQQKHVPPLSNLQTNSPHIQNGLPVSDSTNDNSCNVVDTISLKTMAQDAINRSAIDPNSLNQQQTSSIDLRQPQSQKSLLQHFNSETNTNQQQLTSQQQQQLQNNSLAATTGSNNGTSTGSGLMNVANATGQPISGNAKTHACQPQQMATTEAHIPTLLGVTPLGPTPLQKEHQMQFQMMEAAYYHLPQPMDTEKLQTYFHRAPVLTPSHYPQAQMPIYDTVEFYQRLSTETLFFVFYYMEGSKAQYLAAKALKKQSWRFHTKYMMWFQRHEEPKIINDDYEQGTYIYFDYEKWSQRKKEGFTFEYKYLEDKELN

Target ORF information:

RefSeq Version	NM_001273783.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster CCR4-NOT transcription complex subunit 3 (Not3), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273783.1

ORF Insert Sequence:

ATGGCTGCGA CGAGAAAATT GCAAGGCGAA ATTGATCGAT GTTTAAAAAA GGTAGCAGAG 
GGCGTTGAAA CGTTTGAGGA TATTTGGAAA AAAGTACACA ATGCGACAAA CACCAATCAG 
AAGCAAAAAC ATCTGCAGGA GAAGTATGAG GCAGACCTCA AAAAGGAAAT CAAGAAATTG 
CAACGACTTC GCGACCAAAT TAAATCATGG ATTGCATCAG CTGAAATTAA GGATAAGAGC 
TCCCTTCTTG AGAATCGACG TCTCATTGAA ACACAAATGG AACGCTTTAA GGTGGTTGAG 
CGCGAGACGA AAACAAAAGC CTACTCCAAG GAAGGCTTGG GGGCTGCACA AAAGATGGAT 
CCTGCCCAGC GTATTAAAGA CGACGCACGC AATTGGTTAA CCAGCTCAAT AAGTTCCCTT 
CAAATACAAA TTGACCAGTA TGAGAGCGAG ATCGAATCGT TGTTGGCAGG TAAAAAGAAA 
CGTTTAGATC GAGACAAGCA GGAGCGAATG GATGACCTAC GAGGAAAGTT GGACCGCCAT 
AAGTTCCACA TAACAAAGCT GGAAACTCTG CTTAGGCTGC TTGACAATGA CGGCGTCGAA 
GCAGAGCAGG TAAACAAGAT CAAAGACGAT GTCGAATACT ACATTGATTC ATCGCAAGAG 
CCAGACTTTG AGGAAAATGA GTTCATATAC GATGATATAA TTGGTCTTGA TGAAGTGGAG 
CTAAGTGGCA CGGCTACCAC GGACAGCAAC AACAGCAACG AGACAAGCGG TTCTCCAAGC 
AGTGTCACAT CCGGAGGCAG TCCATCACAG TCCCCTGTCA CGGTTCAACA AATTTTAAAC 
ACTTCGTCGC AGGGGGCAGC TAGTAGTGGT AGCAGCGCAG CTAGCGCAGC ACTGTTTCAG 
CAGCAGCTGA CAGCGGCACA ATCGAATGGC AACAATGTGG GCTACGCGAG CGACACAAGT 
GCAGCTTCTT CGTCTGCTAC AACATCTACA GATCCTGCAG GCGGCACAGT TGCTGTAAAT 
TGTGTTGGAG GGCTAGCCGA TAAACGCAAC AAGAGCAGCG AAAGCAATGC CCTAAAATTG 
AAACCACAAC CTCATCAACT TATAAAACCT ACCCCTGTAC GAGCCACGGC CAAGCTGCCT 
CTGAGCAGTG ATACCCAAGT CAATAAGATT GTCAGTTCAA CTCCCAGCAA AAATCAGCAA 
CAGCTGCCTA CAGCAGCTTC AATCGTTGCA ACCTCCGCTA TGCAATCGCA AAGCAGTATC 
GGAAGTTGCA GCTCCACTGG AGGAACGGGA GCATCTCAAT CAGCTTCTAG TGGTAACAAT 
CCTGGTAACA ATCCTGCGGT TCAGCCAAAT GCTCCAACGC CTGGTCAAAG CGGCATTGCC 
GCTGCAGCAG CTTCGACTAA TGTTGTTTCG GCGACGATTG TGAGCAGCGC CAATGTTCAA 
GGCCAATCTG TGATCCAACC AACACCAACA ATTGCATTTG CTGCAGTAGC AAAACACAAT 
ACATCACTCC TGGAAAATGG TCCTGTTTTG CAACAGCAAC TAGCAGTAAC TCCTACTGTG 
GCAGCTATCG TAGGAGCTGG AACGCAGGCG CAGCAAAAAC ATGTGCCACC ATTATCCAAT 
CTTCAAACAA ATTCCCCTCA CATACAAAAT GGTCTTCCTG TCTCGGACAG TACTAATGAC 
AACAGCTGCA ATGTCGTCGA TACGATTTCT CTAAAAACAA TGGCACAGGA TGCTATTAAT 
CGATCTGCGA TCGATCCTAA TTCACTGAAT CAGCAGCAAA CAAGCAGCAT CGATCTGAGA 
CAGCCGCAGT CGCAGAAATC ACTTCTCCAA CATTTCAACT CTGAAACCAA CACGAATCAA 
CAGCAATTGA CATCGCAGCA GCAGCAACAG CTTCAGAATA ACTCCCTTGC TGCGACCACA 
GGTTCTAACA ACGGAACTTC AACTGGTAGT GGTCTCATGA ATGTAGCAAA CGCTACTGGT 
CAGCCTATCA GCGGCAATGC CAAAACGCAT GCGTGCCAAC CCCAACAGAT GGCCACGACG 
GAAGCGCACA TTCCCACACT GCTTGGTGTG ACACCACTTG GGCCAACACC ACTTCAAAAG 
GAGCACCAAA TGCAGTTCCA AATGATGGAA GCTGCCTACT ATCACCTGCC ACAGCCTATG 
GACACGGAAA AGCTACAAAC GTACTTTCAT CGCGCACCAG TGCTCACGCC ATCGCACTAT 
CCCCAGGCAC AGATGCCAAT ATATGATACA GTTGAGTTTT ATCAACGACT CTCAACGGAG 
ACCCTTTTTT TTGTGTTCTA CTATATGGAG GGCTCCAAGG CCCAGTATCT AGCGGCCAAG 
GCCTTAAAAA AGCAGAGCTG GCGTTTCCAC ACAAAGTATA TGATGTGGTT TCAAAGGCAC 
GAGGAGCCGA AGATTATCAA CGATGATTAC GAACAGGGTA CGTACATCTA TTTTGATTAT 
GAAAAGTGGA GTCAGCGAAA GAAGGAGGGA TTCACCTTTG AATACAAGTA CTTAGAGGAC 
AAGGAGCTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Not3 ( NM_136332.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610176.1 Drosophila melanogaster CCR4-NOT transcription complex subunit 3 (Not3), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa08997
Clone ID Related Accession (Same CDS sequence)	NM_001273783.1 , NM_136332.3
Accession Version	NM_136332.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	CCR4-NOT transcription complex subunit 3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136332.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCGA CGAGAAAATT GCAAGGCGAA ATTGATCGAT GTTTAAAAAA GGTAGCAGAG 
GGCGTTGAAA CGTTTGAGGA TATTTGGAAA AAAGTACACA ATGCGACAAA CACCAATCAG 
AAGCAAAAAC ATCTGCAGGA GAAGTATGAG GCAGACCTCA AAAAGGAAAT CAAGAAATTG 
CAACGACTTC GCGACCAAAT TAAATCATGG ATTGCATCAG CTGAAATTAA GGATAAGAGC 
TCCCTTCTTG AGAATCGACG TCTCATTGAA ACACAAATGG AACGCTTTAA GGTGGTTGAG 
CGCGAGACGA AAACAAAAGC CTACTCCAAG GAAGGCTTGG GGGCTGCACA AAAGATGGAT 
CCTGCCCAGC GTATTAAAGA CGACGCACGC AATTGGTTAA CCAGCTCAAT AAGTTCCCTT 
CAAATACAAA TTGACCAGTA TGAGAGCGAG ATCGAATCGT TGTTGGCAGG TAAAAAGAAA 
CGTTTAGATC GAGACAAGCA GGAGCGAATG GATGACCTAC GAGGAAAGTT GGACCGCCAT 
AAGTTCCACA TAACAAAGCT GGAAACTCTG CTTAGGCTGC TTGACAATGA CGGCGTCGAA 
GCAGAGCAGG TAAACAAGAT CAAAGACGAT GTCGAATACT ACATTGATTC ATCGCAAGAG 
CCAGACTTTG AGGAAAATGA GTTCATATAC GATGATATAA TTGGTCTTGA TGAAGTGGAG 
CTAAGTGGCA CGGCTACCAC GGACAGCAAC AACAGCAACG AGACAAGCGG TTCTCCAAGC 
AGTGTCACAT CCGGAGGCAG TCCATCACAG TCCCCTGTCA CGGTTCAACA AATTTTAAAC 
ACTTCGTCGC AGGGGGCAGC TAGTAGTGGT AGCAGCGCAG CTAGCGCAGC ACTGTTTCAG 
CAGCAGCTGA CAGCGGCACA ATCGAATGGC AACAATGTGG GCTACGCGAG CGACACAAGT 
GCAGCTTCTT CGTCTGCTAC AACATCTACA GATCCTGCAG GCGGCACAGT TGCTGTAAAT 
TGTGTTGGAG GGCTAGCCGA TAAACGCAAC AAGAGCAGCG AAAGCAATGC CCTAAAATTG 
AAACCACAAC CTCATCAACT TATAAAACCT ACCCCTGTAC GAGCCACGGC CAAGCTGCCT 
CTGAGCAGTG ATACCCAAGT CAATAAGATT GTCAGTTCAA CTCCCAGCAA AAATCAGCAA 
CAGCTGCCTA CAGCAGCTTC AATCGTTGCA ACCTCCGCTA TGCAATCGCA AAGCAGTATC 
GGAAGTTGCA GCTCCACTGG AGGAACGGGA GCATCTCAAT CAGCTTCTAG TGGTAACAAT 
CCTGGTAACA ATCCTGCGGT TCAGCCAAAT GCTCCAACGC CTGGTCAAAG CGGCATTGCC 
GCTGCAGCAG CTTCGACTAA TGTTGTTTCG GCGACGATTG TGAGCAGCGC CAATGTTCAA 
GGCCAATCTG TGATCCAACC AACACCAACA ATTGCATTTG CTGCAGTAGC AAAACACAAT 
ACATCACTCC TGGAAAATGG TCCTGTTTTG CAACAGCAAC TAGCAGTAAC TCCTACTGTG 
GCAGCTATCG TAGGAGCTGG AACGCAGGCG CAGCAAAAAC ATGTGCCACC ATTATCCAAT 
CTTCAAACAA ATTCCCCTCA CATACAAAAT GGTCTTCCTG TCTCGGACAG TACTAATGAC 
AACAGCTGCA ATGTCGTCGA TACGATTTCT CTAAAAACAA TGGCACAGGA TGCTATTAAT 
CGATCTGCGA TCGATCCTAA TTCACTGAAT CAGCAGCAAA CAAGCAGCAT CGATCTGAGA 
CAGCCGCAGT CGCAGAAATC ACTTCTCCAA CATTTCAACT CTGAAACCAA CACGAATCAA 
CAGCAATTGA CATCGCAGCA GCAGCAACAG CTTCAGAATA ACTCCCTTGC TGCGACCACA 
GGTTCTAACA ACGGAACTTC AACTGGTAGT GGTCTCATGA ATGTAGCAAA CGCTACTGGT 
CAGCCTATCA GCGGCAATGC CAAAACGCAT GCGTGCCAAC CCCAACAGAT GGCCACGACG 
GAAGCGCACA TTCCCACACT GCTTGGTGTG ACACCACTTG GGCCAACACC ACTTCAAAAG 
GAGCACCAAA TGCAGTTCCA AATGATGGAA GCTGCCTACT ATCACCTGCC ACAGCCTATG 
GACACGGAAA AGCTACAAAC GTACTTTCAT CGCGCACCAG TGCTCACGCC ATCGCACTAT 
CCCCAGGCAC AGATGCCAAT ATATGATACA GTTGAGTTTT ATCAACGACT CTCAACGGAG 
ACCCTTTTTT TTGTGTTCTA CTATATGGAG GGCTCCAAGG CCCAGTATCT AGCGGCCAAG 
GCCTTAAAAA AGCAGAGCTG GCGTTTCCAC ACAAAGTATA TGATGTGGTT TCAAAGGCAC 
GAGGAGCCGA AGATTATCAA CGATGATTAC GAACAGGGTA CGTACATCTA TTTTGATTAT 
GAAAAGTGGA GTCAGCGAAA GAAGGAGGGA TTCACCTTTG AATACAAGTA CTTAGAGGAC 
AAGGAGCTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610176.1
CDS	580..3114
Translation	MAATRKLQGEIDRCLKKVAEGVETFEDIWKKVHNATNTNQKQKHLQEKYEADLKKEIKKLQRLRDQIKSWIASAEIKDKSSLLENRRLIETQMERFKVVERETKTKAYSKEGLGAAQKMDPAQRIKDDARNWLTSSISSLQIQIDQYESEIESLLAGKKKRLDRDKQERMDDLRGKLDRHKFHITKLETLLRLLDNDGVEAEQVNKIKDDVEYYIDSSQEPDFEENEFIYDDIIGLDEVELSGTATTDSNNSNETSGSPSSVTSGGSPSQSPVTVQQILNTSSQGAASSGSSAASAALFQQQLTAAQSNGNNVGYASDTSAASSSATTSTDPAGGTVAVNCVGGLADKRNKSSESNALKLKPQPHQLIKPTPVRATAKLPLSSDTQVNKIVSSTPSKNQQQLPTAASIVATSAMQSQSSIGSCSSTGGTGASQSASSGNNPGNNPAVQPNAPTPGQSGIAAAAASTNVVSATIVSSANVQGQSVIQPTPTIAFAAVAKHNTSLLENGPVLQQQLAVTPTVAAIVGAGTQAQQKHVPPLSNLQTNSPHIQNGLPVSDSTNDNSCNVVDTISLKTMAQDAINRSAIDPNSLNQQQTSSIDLRQPQSQKSLLQHFNSETNTNQQQLTSQQQQQLQNNSLAATTGSNNGTSTGSGLMNVANATGQPISGNAKTHACQPQQMATTEAHIPTLLGVTPLGPTPLQKEHQMQFQMMEAAYYHLPQPMDTEKLQTYFHRAPVLTPSHYPQAQMPIYDTVEFYQRLSTETLFFVFYYMEGSKAQYLAAKALKKQSWRFHTKYMMWFQRHEEPKIINDDYEQGTYIYFDYEKWSQRKKEGFTFEYKYLEDKELN

Target ORF information:

RefSeq Version	NM_136332.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster CCR4-NOT transcription complex subunit 3 (Not3), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136332.3

ORF Insert Sequence:

ATGGCTGCGA CGAGAAAATT GCAAGGCGAA ATTGATCGAT GTTTAAAAAA GGTAGCAGAG 
GGCGTTGAAA CGTTTGAGGA TATTTGGAAA AAAGTACACA ATGCGACAAA CACCAATCAG 
AAGCAAAAAC ATCTGCAGGA GAAGTATGAG GCAGACCTCA AAAAGGAAAT CAAGAAATTG 
CAACGACTTC GCGACCAAAT TAAATCATGG ATTGCATCAG CTGAAATTAA GGATAAGAGC 
TCCCTTCTTG AGAATCGACG TCTCATTGAA ACACAAATGG AACGCTTTAA GGTGGTTGAG 
CGCGAGACGA AAACAAAAGC CTACTCCAAG GAAGGCTTGG GGGCTGCACA AAAGATGGAT 
CCTGCCCAGC GTATTAAAGA CGACGCACGC AATTGGTTAA CCAGCTCAAT AAGTTCCCTT 
CAAATACAAA TTGACCAGTA TGAGAGCGAG ATCGAATCGT TGTTGGCAGG TAAAAAGAAA 
CGTTTAGATC GAGACAAGCA GGAGCGAATG GATGACCTAC GAGGAAAGTT GGACCGCCAT 
AAGTTCCACA TAACAAAGCT GGAAACTCTG CTTAGGCTGC TTGACAATGA CGGCGTCGAA 
GCAGAGCAGG TAAACAAGAT CAAAGACGAT GTCGAATACT ACATTGATTC ATCGCAAGAG 
CCAGACTTTG AGGAAAATGA GTTCATATAC GATGATATAA TTGGTCTTGA TGAAGTGGAG 
CTAAGTGGCA CGGCTACCAC GGACAGCAAC AACAGCAACG AGACAAGCGG TTCTCCAAGC 
AGTGTCACAT CCGGAGGCAG TCCATCACAG TCCCCTGTCA CGGTTCAACA AATTTTAAAC 
ACTTCGTCGC AGGGGGCAGC TAGTAGTGGT AGCAGCGCAG CTAGCGCAGC ACTGTTTCAG 
CAGCAGCTGA CAGCGGCACA ATCGAATGGC AACAATGTGG GCTACGCGAG CGACACAAGT 
GCAGCTTCTT CGTCTGCTAC AACATCTACA GATCCTGCAG GCGGCACAGT TGCTGTAAAT 
TGTGTTGGAG GGCTAGCCGA TAAACGCAAC AAGAGCAGCG AAAGCAATGC CCTAAAATTG 
AAACCACAAC CTCATCAACT TATAAAACCT ACCCCTGTAC GAGCCACGGC CAAGCTGCCT 
CTGAGCAGTG ATACCCAAGT CAATAAGATT GTCAGTTCAA CTCCCAGCAA AAATCAGCAA 
CAGCTGCCTA CAGCAGCTTC AATCGTTGCA ACCTCCGCTA TGCAATCGCA AAGCAGTATC 
GGAAGTTGCA GCTCCACTGG AGGAACGGGA GCATCTCAAT CAGCTTCTAG TGGTAACAAT 
CCTGGTAACA ATCCTGCGGT TCAGCCAAAT GCTCCAACGC CTGGTCAAAG CGGCATTGCC 
GCTGCAGCAG CTTCGACTAA TGTTGTTTCG GCGACGATTG TGAGCAGCGC CAATGTTCAA 
GGCCAATCTG TGATCCAACC AACACCAACA ATTGCATTTG CTGCAGTAGC AAAACACAAT 
ACATCACTCC TGGAAAATGG TCCTGTTTTG CAACAGCAAC TAGCAGTAAC TCCTACTGTG 
GCAGCTATCG TAGGAGCTGG AACGCAGGCG CAGCAAAAAC ATGTGCCACC ATTATCCAAT 
CTTCAAACAA ATTCCCCTCA CATACAAAAT GGTCTTCCTG TCTCGGACAG TACTAATGAC 
AACAGCTGCA ATGTCGTCGA TACGATTTCT CTAAAAACAA TGGCACAGGA TGCTATTAAT 
CGATCTGCGA TCGATCCTAA TTCACTGAAT CAGCAGCAAA CAAGCAGCAT CGATCTGAGA 
CAGCCGCAGT CGCAGAAATC ACTTCTCCAA CATTTCAACT CTGAAACCAA CACGAATCAA 
CAGCAATTGA CATCGCAGCA GCAGCAACAG CTTCAGAATA ACTCCCTTGC TGCGACCACA 
GGTTCTAACA ACGGAACTTC AACTGGTAGT GGTCTCATGA ATGTAGCAAA CGCTACTGGT 
CAGCCTATCA GCGGCAATGC CAAAACGCAT GCGTGCCAAC CCCAACAGAT GGCCACGACG 
GAAGCGCACA TTCCCACACT GCTTGGTGTG ACACCACTTG GGCCAACACC ACTTCAAAAG 
GAGCACCAAA TGCAGTTCCA AATGATGGAA GCTGCCTACT ATCACCTGCC ACAGCCTATG 
GACACGGAAA AGCTACAAAC GTACTTTCAT CGCGCACCAG TGCTCACGCC ATCGCACTAT 
CCCCAGGCAC AGATGCCAAT ATATGATACA GTTGAGTTTT ATCAACGACT CTCAACGGAG 
ACCCTTTTTT TTGTGTTCTA CTATATGGAG GGCTCCAAGG CCCAGTATCT AGCGGCCAAG 
GCCTTAAAAA AGCAGAGCTG GCGTTTCCAC ACAAAGTATA TGATGTGGTT TCAAAGGCAC 
GAGGAGCCGA AGATTATCAA CGATGATTAC GAACAGGGTA CGTACATCTA TTTTGATTAT 
GAAAAGTGGA GTCAGCGAAA GAAGGAGGGA TTCACCTTTG AATACAAGTA CTTAGAGGAC 
AAGGAGCTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Not3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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