Nipped-A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nipped-A
Entrez Gene ID	35483
Full Name	CG33554 gene product from transcript CG33554-RE
Synonyms	41Ah,CG10549,CG2905,CG33554,Dmel\CG33554,GroupV,Nip-A,NipA,Nipped A,Nipped[A],TRA1,TRRAP,Tra-1,Tra1,dTRRAP,dTra1,dmTRRAP,l(2)41Ah,l(2)C123,l(2)EMS34-12,tra1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 41E1-41E3\|2-55.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103722.3	NP_001097192.2	Nipped-A, isoform E
NM_001316406.1	NP_001303335.1	Nipped-A, isoform F

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	trrap	XP_001919276.4
Drosophila melanogaster (fruit fly)	Nipped-A	NP_001097192.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005533	XP_556172.2
Kluyveromyces lactis	KLLA0F22066g	XP_456065.1
Neurospora crassa	NCU01379	XP_960936.2
Arabidopsis thaliana (thale cress)	AT4G36080	NP_680770.2
	TRRAP	XP_001093586.2
Bos taurus (cattle)	TRRAP	NP_001179066.1
Mus musculus (house mouse)	Trrap	NP_001074831.1
Xenopus tropicalis (tropical clawed frog)	trrap	XP_004918032.1
Homo sapiens (human)	TRRAP	NP_003487.1
Canis lupus familiaris (dog)	TRRAP	XP_005621163.1
Saccharomyces cerevisiae (baker's yeast)	TRA1	NP_011967.1
Magnaporthe oryzae (rice blast fungus)	MGG_09523	XP_003712218.1
Eremothecium gossypii	AGOS_AER393C	NP_985248.2
Schizosaccharomyces pombe (fission yeast)	tra1	NP_595777.1
Arabidopsis thaliana (thale cress)	AT2G17930	NP_179383.3
Pan troglodytes (chimpanzee)	TRRAP	XP_001136582.1
Rattus norvegicus (Norway rat)	Trrap	NP_001099377.2
Gallus gallus (chicken)	TRRAP	XP_004945166.1
Caenorhabditis elegans (roundworm)	trr-1	NP_001022032.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nipped-A seems to be a superordinate regulator of intestinal stem cell (ISC) proliferation, possibly by coordinating different processes including modifying the chromatin landscape of ISCs and progenitors
Title: Nipped-A regulates intestinal stem cell proliferation in

Nipped-A facilitates assembly of the Notch activator complex and target gene transcription
Title: Nipped-A, the Tra1/TRRAP subunit of the Drosophila SAGA and Tip60 complexes, has multiple roles in Notch signaling during wing development.

The following Nipped-A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nipped-A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12382	NM_001103722.3 Latest version!	Drosophila melanogaster Nipped-A, transcript variant E (Nipped-A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa41281	NM_001316406.1 Latest version!	Drosophila melanogaster Nipped-A, transcript variant F (Nipped-A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nipped-A ( NM_001103722.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097192.2 Drosophila melanogaster Nipped-A, transcript variant E (Nipped-A), mRNA.

Price & Availability↑

CloneID	OFa12382
Clone ID Related Accession (Same CDS sequence)	NM_001103722.3
Accession Version	NM_001103722.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nipped-A, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001103722.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGTTA TTGAAAATGT ACCGGTGAAT ACATTTCGGA ATTATTTAAA TATTTTAAAT 
GATTCCTCTT CTAAAGACGA ACTTAAGCTG AAAGCTACTC AAGAGCTAAG TGAACACTTT 
GAAATGATAA TGCAAAGTCC GGCATATCCG TCGTTCTTAG ATAATTCTTT AAAAATATTT 
ATGCGGATAC TTCAGGATGG CGAACCTCAG TTCATTCAAG AAAATACAAT GCAACACATA 
CGTAAACTGA TTTTAGAGAT GATTCATCGA TTGCCTATAA CAGAAAGCTT AAGGCAACAT 
GTAAAGACAA TAATAACAAT GATGCTTAAA ATACTTAAAA CTGACAACGA AGAAAATGTT 
CTTGTATGTC TTCGCATTAT AATTGAACTG CATAAGCATT TTCGTCCATC ATTTAATTCT 
GAAATTCAAC TATTTTTGGG ATTTGTTAAG GAAATCTATA CAAATCTTCC TAACCATCTT 
ACATCTATAT TTGAAACATC GAATGATGTT TGGGTTACCG ATTTAAAGGA CTTAAATTTG 
GAAGTACTTT TATCCGAATC ATATTCTGTT AGAACTATCC ATGTCGAAAA GGCACTTGAT 
TCAAATTCTC AACAACAAAT TTATAATCTC CTTCCTCGTG GAATATTATC TTTGAAAGTT 
TTACAAGAGC TCCCAATAAT AGTAGTACTA ATGTACCAAA TATATAAAAA CGCAGTACAT 
CAAGAAGTTT CTGAATTCAT TCCTCTTATA TTGACTACTA TTAATTTGCA ACCAACAGTA 
ACACGAAGAA ATTCTCCTCA GAAAGAAATA TATGTCGAAT TCATGGGTGC ACAAATTAAA 
ACTCTGTCAT TTTTGGCGTA CATTGTCCGA ATATTTCAGG AAGTGGTAAT AGCTAGTTCA 
TTATCTGTAA CCAGCGGAAT GCTCAACCTT ATGAAAAATT GTCCCAAGGA AGCCGCTCAT 
CTCAGAAAAG AACTTTTAAT TGCTGCCCGT CATATATTTG CTACAGATTT ACGTCAAAAA 
TTTATTCCAT CAATCGAACA ATTGTTTGAT GAGGATTTGC TTATTGGAAA AGGAGTTACA 
TTGGATTCTA TTCGGCCATT GGCGTATTCA ACTTTAGCTG ACCTAGCTCA CCACGTTCGC 
CAAAGCCTAA ATATAGACGT TTTAATAAAG GCAGTGAATC TCTTTTCAAA AAATGTTCAT 
GATGAATCAC TTGCTGTGGG AATACAAACT ATGTCATGCA AGCTTTTGTT AAATCTAGTT 
GATTGTTTAC GTCATCACAG TGAAACAGAA CCACAAAGAT CAAAAGCTCT ACTCTCAAAA 
CTGTTAAAAG TGTTTGTAAA AAAATTTGAA ACTATAGCTA AAATTCAGCT TCCATTAATA 
ATTCAAAAGT GCAAAGGGCA CGCATTTTCT GGAGCATTGG TAAATTCATC TGGCAACGCT 
TCCTTATCAC ACATTAACGC GCCAGATCTA AAAGACGACA TATCCAACAT CCAAGTATCT 
GCATCTGGAT CACAATGGAT ATATTCCGTC AATGTCGCTG AATTTCGAAG CTTAGTAAAA 
ACCCTTGTTG GTGGAGTAAA AACAATAACT TGGGGTTTTT TTAACTCAAA GTTTCAGCTA 
ACTGATACCA AACTGGCCAA CCATGAAAAA ATTTTTGGAC CTGAAATCGT ATGTTCTTAC 
ATCGACTTGG TTTACTATGC GATGGAGGCT CTAGATATAT ACACTATAAA CGTTAATCCA 
AATCAACAGA GAACGTCTGG GTTGATTTCC AGAAGTAAAG AAGAAAAAGA AGTCTTGGAG 
CATTTTAGTG GCATATTTTT AATGATGCAC TCCCAAAACT TTCAAGAAAT ATTTTCTACT 
ACGATTAATT TTTTGGTGGA AAGGATCTAC AAAAATCAGT CCTTACAAGT GATAGCGAAC 
TCCTTTTTAG CAAATCCTAC TACTTCACCT CTGTTTGCCA CTGTTCTAGT GGAATATCTT 
TTGAACAAAA TGGAAGAAAT GGGATCAAAC TTGGAACGCT CCAACTTGTA TCTACGATTA 
TTCAAGTTGG TATTCGGCAG CGTAAGTCTT TTTCCAGTCG AAAATGAACA GATGTTGCGT 
CCACATCTTC ATAAAATAGT CAATAGATCC ATGGAATTGG CTTTGATATC GGAGGAGCCG 
TATAACTATT TTTTACTTCT GAGGGCATTG TTTAGATCGA TTGGAGGAGG AAGCCATGAC 
CTATTATACC AAGAGTTTCT GCCACTTTTA CCAAACTTAT TAGAAGGATT AAATCGCCTA 
CAGAGTGGAT TTCATAAGCA GCACATGCGA GATCTTTTCG TGGAACTTTG CCTTACAGTT 
CCGGTCCGCT TGTCATCTTT ACTGCCCTAT TTACCAATGT TGATGGACCC ATTAGTTTCT 
GCGTTAAACG GATCTCCAAC ATTAATAAGT CAGGGCCTGC GAACCTTAGA GCTTTGCGTT 
GACAATTTGC AGCCAGACTT TTTATATGAT CATATACAAC CAGTAAGAGC TGCTTTAATG 
CAAGCATTGT GGAAAACTTT AAGAAATCAG GATAACGCCG CTTTGGTTGC CTTTCGCGTT 
CTAGGAAAAT TTGGTGGCGG CAATCGAAAA ATGATGGTGG AGCCACAAGC TTTGTCGTAT 
ATTATAAACG ACAAGCCCAC CATTTCCATT GTAACTTATT TTCAGGAATA CGAAACACCA 
ATAGATTTTC CTGTTGATGA AGCGATTAAG TCCGCCTTTA GAGCATTGGG ATCAAATTCT 
ACTGACCAGT TTTATAGGAG GCAAAGCTGG GAAGTAATCA GGTGCTTCTT AGCTGCTTTT 
ATTAGTTTGG ATGATGAAAA ACATATGCTG CTGAAACTTT TTACACATGT AGATTTTGTT 
GAAAATAAAA TTATGAATTG GTCTACGTTT CAACATAAAG CCGGGAACGA AACTGTACGA 
GAAACTCATC AGACAGCCCT CATAGGTATG TTGGTTGCAT CAGCAACAAA GGACTTGCGT 
GATTCAGTGT GCCCAGTAAT GGCCGCTGTT GTGAGGCATT ATACTATGGT AGCTATTGCA 
CAGCAGGCCG GACCTTTTCC ACAAAAAGGC TACCAAGCCA CTCATGGAAT TGACCCCATG 
ATATTAATAG ATGCTTTAGC CTCTTGCATG GGTCATGAAG AAAAGGAGTT ATGCAAACCC 
GGAATAGCTT GTATGGGTAT TATACTTGAC ACTGCAACAA ATATTATGGG TAATAAAGAC 
AGAGCGTGTA AATTGCCCAT AATACAGTAT CTAGCAGAAA AAATGGTATC CTTATGCTAT 
GATCGGCCAT GGTACTCAAA AGTAGGCGGT TGCCAAGCAA TTCAATTTTT ATGCAAACAT 
ATGTCTTTGC GAGCCCTTTT TCAAAATCTC TTTAATTTTT TAAAAGCTTT CATGTTTGTA 
TTAATGGACC TAGAGGGAGA CGTTTCTAAT GGGGCTATTG AAATAACAAA AAGTTATATG 
AAAAGCATGT TGGAAATTTG TTTAACGCCA ATAAATGAAT GTTATAAGAA TATAGATCTA 
AAGGACTTAC AAGCCAAAGC CACTTATGAA GTTATTCACG AATTAGTGCG GCACATCACA 
AGCCCTAATA CCATTGTTAG AGAAGAATCT ATGGTACTAC TTAAACATAT TGGTACAATA 
CAATCCAAAA CCGTTTCTGA AGTTATGGAT CCTCATAAAG ACGTTCTCGC TGACATTATA 
CCGCCAAAGA AGCATTTATT GAGACATCAA CCCGCAAATG CGCAAATCGG ACTTATGGAT 
GGCAATACAT TCTGCACAAC TTTGGAGCCA AGACTTTTTA CAATTGATTT GACGAATACC 
TACCACAAAT TATTTTTTCA CGAGTTACTT ACACTAAGTG AAGCCGAAGA TGCCACTTTA 
GCAAAGTTGG ACTGTTACAA AAACGTTCCG AATCTTATAC CACTTAGGAC TAGTGCGTTG 
CGAGCTTTAG CAGCATGCCA CTATATAAGT GATATCGGAT ACAAAGAAAA AATAATAAAT 
ATAATTTTTA AAGTAATGGA AAGTGATAAA TCAGAACTTC AGACAACTGC GTTTCATTGC 
ATGAAGCATT TTATAACTGG AGTTACTCTG GAAAAAGAAA AGGTACAATC TGCAATGCGC 
CCACTGCTAT TAAAATTGGG CGACCACAGA AATTTATCGA TACCAGCAAT AAAACGCTTA 
TCTTACTTTA CTCAGATCTT TCCTCAAATG TTCAATGAGA AGTTAAGTGA GCAAATTTTG 
CAACATTGCT CTAAAATTAT GGAAATATTT GTTAGTGAAT ACAAATCCAC AAGTCCAAAC 
GTTAATTTTT TTGCATCATC GAAAGGTGGA GAATATGAAC AGAAAATTGT CATACTAATC 
GAAATGTTTT TCTATATATC AGCATCAGTA AAATACATTG AAAAGCTGTG CCAACTTGTT 
TTAAAAACGG AAAAAAACTT AATGATTGAG GCATCGAGTC CATATCGAGA AGCCCTAATT 
AAGTTTCTTC AACGATTTCC AACAGAAACT GTGGATTTAT TTTTAACGGA ATCTCTTATG 
ATAGATCCGC AATGGAATCG TCTTTTTATA TACTTGTTGA AACACGAAAC GGGAGTATCA 
TTTCGAGCAG TTATTAAAAG TAGTCGGTAT AATAATTTAA TACATTATTT AAATACACAC 
ACAGAATTTC CAGAAGCACT CAAATATGAA ATTCAGCACC AAGCCGTTTT AATAATTTTT 
ACATTAATGG AATCAGACGA TCAATGGATA CCAACACGAC AAGATATTGT TGACGCCCTT 
AAAAATTGTT GGCAGAATTA TTTAAGTACA TTAAGCTCTG AAGACGTTCT GTGTGACTTA 
TGGCATTTGA TTGGAAAAAT ACTTTTGCAT TATTTTTCTA ACAATACAAA TGACATTGAA 
CTGCTGTTTC AATTATTACG TGCTTTATGC TTCCGATTTA TACCGGATGT CTATTTCCTG 
CGGGACTTCT TGCAACACAC AGTAGCACAA AGTTTTACGG TTAACTGGAA ACGAAACGCA 
TTCTTTTACT TTGTTGAAAA CTTTAATAAC AGCTTTCTTT CAGAAGAGCT AAAGGCAAAA 
ATAATAACAG CAGTCATAAT ACCCTGTTTT GCTGTAAGTT TCGATAAAGG TGAAGGAAAT 
AAACTAATTG GGGCTCCTCC GACCCCGTAT CAAGAAGATG AAAAAAATAT TGTGTCCGTT 
TTTATTAATA AAGTGTTTGA CCCAGATAAA CAGTATGATG ATGCAGTAAG AATTGCTCTA 
TTACAACTAG CATGCTTATT GGTAGAACGT GCATCTCAGC ACATACATGA CGGAGATGCT 
AATAATAAGC GCCAAGGCAA TAAGCTTAGA CGACTCATGA CCTTTGCCTG GCCCTGTTTG 
CTGAGTAAAA GTTCAGTGGA TCCTACTGCA CGATACCACG GTCACTTATT ACTTTCGCAT 
ATAATCGCAA GATTGGCCAT TCACAAGAAA ATAGTTTTAC AAGTATTTCA TTCCTTGTTG 
AAAGGACACG CATTAGAGGC CAGATCTATT GTTAAACAGG CACTTGATGT TCTAACGCCA 
GCTATGCCGT TGCGAATGGA GGATGGAAAT ACTATGCTAA CACACTGGAC AAAAAAAATT 
ATAGTTGAAG AAGGTCACGC TATGCAGCAG TTATTTCATA TTTTGCAGCT CATTATTCGT 
CATTATAAAG TGTATTTTCC GGTTCGACAT CAACTAGTGC AACACTTGAT TAACTATATG 
CAGCGCCTTG GGTTTCCTCC TACTGCATCT ATTGAACATA AAAAACTTGC TGTGGACTTA 
GCAGAAGTTA TTATAAAATG GGAACTTCAT CGTATTAAGG ATGATCGTGA AACTAAAACA 
GATGGAACCG AAGAGGAGTT AATACAGGAA AGCTCAGTAA AACGATCAGG GATCGATTTA 
GTAGAAACTC GAAAAAAGTC ATTCGATATT ATAAGAGAAA CAACAGTCCA AGGAGTGGGA 
TCACATACCA AGCCTGATGA CATACTGCGA TCAATAGATA AATCCTATTG TGATACGGTT 
TTAAATTTCT TGATACGTTT AGCATGTCAG GTTAATGACC CGCAAGCCCC AATACTTTCG 
CCAGGAGAAA GTTTGTCTCG AAGGTGCGTT ATGCTATTAA AAATGGCGAT GCGTCCCGAG 
ATCTGGCCTC AACCATTTGA TATTAAGCTT AATTGGCTTG ATAAAGTTCT TGCTACAGTG 
GAAACCCCTC ATCACAACTT AAACAACATA TGTACTGGAA TAGATTTTTT AACATTTTTA 
ACTACTATAC TAAGTCCAGA TCAGTTGGTG TCAATTATAC GACCGGTTCA ACGAGGCCTG 
TCCTTGTGTA TAATTCATCA AAATACACGA ATTGTGCGGT TAATGCATAT GTTTTTGACG 
CGAATAATGG CAATTTTTCC ACCTGACACC CAACACAAGC ATGAAGATCT TGATTTGTTA 
TATACCGCTG TTAGTAAAAT GATCGCTGAA AACCTAACAA GTTACGAAAA GAGTCCACAA 
CCGAATGCTT CTTCGCTTTT TGGTACCTTA ATGATTTTGA AAGCGTGCAC TACTAATAAT 
GCAAGTTATA TTGATCGAAT TTTAGTCCAG TTTATAAGGG TTCTGAATCA TCTTACAAGG 
GATCATATTA ACACCATTGG TGGTAACACT GTTATTAGCC AATCTCCTGA TTCAAATGCT 
TTACCTTTAG AACTCTTAGT TCTCTCTTTG GAATTAATCA AGAATAGGAT CTTTGTAATG 
AGTGTGGAAA TCAGAAAACT TTTCATAGGC ACCATTTTGG TTAGTCTTAT AGAAAAGAGC 
ACGGAGGTAA AAATTATAAA ATGTATAATT AAAATGCTGG ATGAATGGAT TAAAACGAAA 
GAGCCAAATG TTATGACACA AGTTCCTTCT ATTCGCGAAA AGTCGGCCTT GCTGGTAAAG 
TTAATGCAAA ACGTTGAAAA GAAATTTACT GATGAAATAG AGCTTAATAT ACAATTCTTG 
GAAATCATAA ATTTTATATA TAGAGATGAA ATTCTTAAAC AAACTGAGTT AACAAACAAA 
CTAGAGGGAG CTTTTTTAAA CGGATTACGT TTTCAAAATC CAAACGTACG CTCGAAATTT 
TTTGAGATTT TAGACTCATC AATGCGGCGT AGACTTCACG ATCGATTGTT ATATATAATT 
TGTTCCCAAG CTTGGGACAC AATTGGTTCC CATTATTGGA TAAAACAATG CATTGAATTG 
CTTATTTTAA CAGCCAATAC AATGATGCAA ATTCAATGTT CAAATGAACA ATTTAAAATA 
CCCAGCATTA CTTCAGTCAT TCCAGTGAAT TCATCAGAAA CACAGGAAAA TTCCTTTGTA 
TCCTTCTTAT CCTCTCATTC CGAATCTTTT GACATTATAC AAACTGTTGA TGATAAAGAC 
GACGTGTATG ATATCGATTT AAATGCTGAT CGCAAAGAAG ATTGTCAACA AATACTACCA 
AATCGACGTG TTACTCTTGT TGAACTAGTT TACAAGCAAG CTGAATTTTT AGAAGCAAAC 
CGAAATATTA GGACCGACCA GATGCTTGTC GCCACATCTC AGCTATGTCA TATTGATACA 
CAGTTAGCTC AAAGCGTATG GTTATCTATG TTTCCACGTA TTTGGAGTAT ATTCACTGAA 
GATCAAAGGT GTAATATCAC AAAAGAACTG ATTCCCTTTT TATCGTCTGG AACTAACGTT 
AATCAAAAAG ACTGCCATCC AAGTACATTA AATACTTTTG TAGAGAGTTT AACTAAATGT 
GCGCCACCCA TATATATTCC ACCTAATTTA TTAGCATACC TAGGCAAATC TCATAACCTA 
TGGCATAGAG CTATACTTGT TTTAGAAGAT ATGGCCGTTA ATCAATCAAT GCAATCCAAG 
GATATTGATG GCGGTGAAAA TCAATTCTCT GACTTGGATG TACAACAATC AAATAATATA 
TTTGATTCAC TTTCAAAAAT GTATTCTTCG ATGCATGAGG AAGATCTTTG GGCTGGCCTA 
TGGCTTAAAT TTGCACACTA CCCGGAAACA AATATAGCTG TTTCGTATGA GCAAATGGGA 
TTTTTCGAAG AAGCCCAAGG TGCCTATGAT CTAGCAATGA CCAAATTTAA ACAAGATCTA 
AGTAATGGTG TAGTTAATAC ATATGTTAAT AGTGAATTAT TATTGTGGGA AAATCACTGG 
ATGCGATGTG CTAAAGAATT GAACCAATGG GACATTTTAC TGGACTATGC CCAAACTAAT 
AAGGACAAAA ATATGTTTTT GATTCTGGAA AGTTCGTGGC GCGTACCTGA TTGGAATTTG 
ATGAAAATCG CACTGGCTAA AACAGAACAA TGCTATTTAA AACACTACGG CTTTAAAATC 
AACCTTTACA AAGGGTATTT GAGTATTCTC CACCAAGAAG AAAGGCAAAC AGGCAATATC 
GAACGATATG TTGAAATTGC ATCCAGCTTA TGCATTCGTG AATGGCGTCG ATTGCCGAAC 
ATAGTTTCAC ATATTCATTT GCCATATCTT CAAGCATCAC AACAAATTAT GGAGCTTCAT 
GAAGCAAGTC AAATCCATCA GGGACTTGCT CAATCGCGCA ACAATTCACT TCACGATATG 
AAAGCTATCG TGAAAACTTG GCGTAATCGT TTACCTATTA TTTCTGATGA CTTATCGCAT 
TGGAGTGACA TATTTACATG GAGACAACAT CACTACCAAA TAATAACACA ACACCTAGAA 
CAACAATCGG ATCAAGGAAG TACAATGCTA GGAGTTCACG CATCAGCACA AGCTATAATT 
TCTTTTGGAA AAATAGCTCG GAAACACAAT TTGACTGGTG TTTGTCAGGA GACGTTGTCC 
AGGATATATA CAATTCCGTC TGTTCCTATC GTGGATTGTT TTCAGAAAAT TCGGCAGCAA 
GTAAAATGCT ACCTGCAAAT GCCCTCAACA TCTGGAAAAA ATGAAATTAA TGAAGCCTTG 
GAAGTAATTG AGTCCACGAA TTTAAAATAC TTCACTGGTG AAATGAATGC TGAATTTTAC 
GCTCTAAAGG GGCTATTATT AGCACAAATT GGAAGATCAG AAGAGGCTGG AAAATCATTT 
AGTGTTGCTG CTCAGCTTCA TGATGGTCTT ACCAAAGCCT GGGCAATGTG GGGTGACTAT 
ATGGAACAAA TATTTTTAAA AGAAAGGAAA ATCACATTAG CCGTCGATGC TTTAATTTGT 
TATTTACAAG CAAGCAGAAA TCAAATTGAA AGCAAAACCC GAAAATATAT TGCAAAAGTT 
TTGTGGTTTC TGTCTTATGA TAATAATACT AAAATCCTCA TAAGCACTTT AGAAAAGCAT 
GTGGCAGGCA TTCCACCCTC TTATTGGCTA CCATGGATTC CTCAGTTGCT CTGTTGCTTA 
GAACAGTTCG AAGGGGATGT TATATTAAAT CTCTTAAGCC AAATTGGACG CCTTTATCCT 
CAAGCGGTAT ATTTCCCGAT TCGGACTTTA TATTTGACTT TAAAAATCGA ACAACGCGAA 
AAACATAAAA CTGCTGAACA GGCTGTAAAA AGTTCATGCT CGAACATCGA TGGAACTACT 
TTAAGCTTTG GAAGGGGAGC AAGTCACGGA AACATTCCAT CAATAAATCC CATTAAAGCA 
ACTCCGCCCA TGTGGCGCTG CTCTAAGGTG ATGCAATTAC AGAGAGAAGT ACATCCAACA 
ATATTAAGTT CATTGGAAGG AATTGTAGAC CAAATGGTTT GGTTTAGAGA AAGCTGGACA 
GAGGAAGTTC TTCGACAACT ACGCCAAGGC CTAATTAAAT GCTATGCCAT AGCCTTTGAA 
AAAAGGGATA CTGTTCAACA TTCTACCATA ACACCTCACA CGTTGCATTT TGTCAAAAAG 
CTGGGTTCTA CGTTTGGCAT TGGAATAGAA AATGTTCCGG GATCAGTAAC CTCCTCAATT 
TCTAATTCAG CAGCCTCGGA GTCTCTTGCT CGACGCGCCC AAGTTACTTT TCAAGATCCA 
GTATTTCAAA AAATGAAGGA GCAATTCACT AATGACTTCG ATTTTTCAAA ACCTGGTGCC 
ATGAAATTGC ACAACTTGAT ATCAAAATTA AAAACATGGA TAAAAGTCCT GGAGACTAAA 
GTTAAAAAAT TACCCACGTC CTTTTTGATA GAAGACAAGT GTAGGTTTTT ATCAAACTTT 
AGTCAGAAGA CAGCTGAGGT TGAACTTCCT GGAGAATTGT TAATTCCCTT ATCATCTCAT 
TATTATGTAA GAATCGCAAG ATTCATGCCG CGTGTGGAAA TTGTACAAAA AAATAACACA 
GCAGCGCGTA GGTTATATAT AAGAGGTACT AACGGGAAAA TCTATCCGTA CCTTGTAGTT 
CTTGATTCAG GTTTGGGAGA TGCTCGCCGA GAGGAAAGAG TTTTGCAGTT AAAACGCATG 
TTGAATTACT ATTTAGAAAA ACAAAAAGAG ACAAGTCGAA GATTTCTTAA CATAACGGTA 
CCAAGGGTTG TCCCGATATC GCCCCAAATG AGATTGGCAG AAGATAACCC AAACAGTATT 
TCATTGTTAA AAATATTTAA AAAATGCTGT CAAAGTATGC AGGTTGACTA CGACATGCCA 
ATAGTTAAGT ATTATGACCG TCTTTCTGAA GTACAGGCAA GAGGCACTCC AACTACACAT 
ACCCTATTGA GAGAAATATT CTCTGAAATT CAATGGACTA TGGTCCCAAA AACATTACTA 
AAGCATTGGG CTTTGAAAAC ATTCTTGGCG GCTACTGACT TTTGGCATTT CCGAAAAATG 
CTTACCCTGC AGTTGGCTTT GGCATTTTTA TGCGAACACG CTTTGAATCT TACTCGACTG 
AATGCGGATA TGATGTACCT TCATCAAGAC TCAGGACTTA TGAACATATC TTATTTTAAG 
TTTGATGTAA ATGATGATAA GTGCCAGCTT AATCAACACC GACCTGTACC ATTTCGCCTG 
ACTCCGAATG TTGGTGAATT CATAACACAT TTTGGAATAA CTGGACCTTT ATCTGCAGCA 
ATTGTGGCAA CGGCTCGGTG TTTTATTCAA CCAAATTACA AATTAAGCTC AATATTACAA 
ACCATTTTAA GAGATGAAAT AATAGCCCTG CAAAAAAAAG GATTCAGAGA ATGTAAACTA 
ATCGAAGGCT CTGAAGACCG TTATTCCGAT GGAAATTGTA TGGAGCACTC AGTAAACATT 
GTGAATTCAG CGGTGGATAT CATAATGACG CGTTTTAATA AAATATCTTA TTTTGATAGC 
ATTGAAAATA AGAAGATTTC CGTGCTCGTT CAATCGGCAA CTAACATTGA TAATCTTTGT 
CGTATGGATC CTGCTTGGCA TCCCTGGCTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097192.2
CDS	58..11430
Translation	MSVIENVPVNTFRNYLNILNDSSSKDELKLKATQELSEHFEMIMQSPAYPSFLDNSLKIFMRILQDGEPQFIQENTMQHIRKLILEMIHRLPITESLRQHVKTIITMMLKILKTDNEENVLVCLRIIIELHKHFRPSFNSEIQLFLGFVKEIYTNLPNHLTSIFETSNDVWVTDLKDLNLEVLLSESYSVRTIHVEKALDSNSQQQIYNLLPRGILSLKVLQELPIIVVLMYQIYKNAVHQEVSEFIPLILTTINLQPTVTRRNSPQKEIYVEFMGAQIKTLSFLAYIVRIFQEVVIASSLSVTSGMLNLMKNCPKEAAHLRKELLIAARHIFATDLRQKFIPSIEQLFDEDLLIGKGVTLDSIRPLAYSTLADLAHHVRQSLNIDVLIKAVNLFSKNVHDESLAVGIQTMSCKLLLNLVDCLRHHSETEPQRSKALLSKLLKVFVKKFETIAKIQLPLIIQKCKGHAFSGALVNSSGNASLSHINAPDLKDDISNIQVSASGSQWIYSVNVAEFRSLVKTLVGGVKTITWGFFNSKFQLTDTKLANHEKIFGPEIVCSYIDLVYYAMEALDIYTINVNPNQQRTSGLISRSKEEKEVLEHFSGIFLMMHSQNFQEIFSTTINFLVERIYKNQSLQVIANSFLANPTTSPLFATVLVEYLLNKMEEMGSNLERSNLYLRLFKLVFGSVSLFPVENEQMLRPHLHKIVNRSMELALISEEPYNYFLLLRALFRSIGGGSHDLLYQEFLPLLPNLLEGLNRLQSGFHKQHMRDLFVELCLTVPVRLSSLLPYLPMLMDPLVSALNGSPTLISQGLRTLELCVDNLQPDFLYDHIQPVRAALMQALWKTLRNQDNAALVAFRVLGKFGGGNRKMMVEPQALSYIINDKPTISIVTYFQEYETPIDFPVDEAIKSAFRALGSNSTDQFYRRQSWEVIRCFLAAFISLDDEKHMLLKLFTHVDFVENKIMNWSTFQHKAGNETVRETHQTALIGMLVASATKDLRDSVCPVMAAVVRHYTMVAIAQQAGPFPQKGYQATHGIDPMILIDALASCMGHEEKELCKPGIACMGIILDTATNIMGNKDRACKLPIIQYLAEKMVSLCYDRPWYSKVGGCQAIQFLCKHMSLRALFQNLFNFLKAFMFVLMDLEGDVSNGAIEITKSYMKSMLEICLTPINECYKNIDLKDLQAKATYEVIHELVRHITSPNTIVREESMVLLKHIGTIQSKTVSEVMDPHKDVLADIIPPKKHLLRHQPANAQIGLMDGNTFCTTLEPRLFTIDLTNTYHKLFFHELLTLSEAEDATLAKLDCYKNVPNLIPLRTSALRALAACHYISDIGYKEKIINIIFKVMESDKSELQTTAFHCMKHFITGVTLEKEKVQSAMRPLLLKLGDHRNLSIPAIKRLSYFTQIFPQMFNEKLSEQILQHCSKIMEIFVSEYKSTSPNVNFFASSKGGEYEQKIVILIEMFFYISASVKYIEKLCQLVLKTEKNLMIEASSPYREALIKFLQRFPTETVDLFLTESLMIDPQWNRLFIYLLKHETGVSFRAVIKSSRYNNLIHYLNTHTEFPEALKYEIQHQAVLIIFTLMESDDQWIPTRQDIVDALKNCWQNYLSTLSSEDVLCDLWHLIGKILLHYFSNNTNDIELLFQLLRALCFRFIPDVYFLRDFLQHTVAQSFTVNWKRNAFFYFVENFNNSFLSEELKAKIITAVIIPCFAVSFDKGEGNKLIGAPPTPYQEDEKNIVSVFINKVFDPDKQYDDAVRIALLQLACLLVERASQHIHDGDANNKRQGNKLRRLMTFAWPCLLSKSSVDPTARYHGHLLLSHIIARLAIHKKIVLQVFHSLLKGHALEARSIVKQALDVLTPAMPLRMEDGNTMLTHWTKKIIVEEGHAMQQLFHILQLIIRHYKVYFPVRHQLVQHLINYMQRLGFPPTASIEHKKLAVDLAEVIIKWELHRIKDDRETKTDGTEEELIQESSVKRSGIDLVETRKKSFDIIRETTVQGVGSHTKPDDILRSIDKSYCDTVLNFLIRLACQVNDPQAPILSPGESLSRRCVMLLKMAMRPEIWPQPFDIKLNWLDKVLATVETPHHNLNNICTGIDFLTFLTTILSPDQLVSIIRPVQRGLSLCIIHQNTRIVRLMHMFLTRIMAIFPPDTQHKHEDLDLLYTAVSKMIAENLTSYEKSPQPNASSLFGTLMILKACTTNNASYIDRILVQFIRVLNHLTRDHINTIGGNTVISQSPDSNALPLELLVLSLELIKNRIFVMSVEIRKLFIGTILVSLIEKSTEVKIIKCIIKMLDEWIKTKEPNVMTQVPSIREKSALLVKLMQNVEKKFTDEIELNIQFLEIINFIYRDEILKQTELTNKLEGAFLNGLRFQNPNVRSKFFEILDSSMRRRLHDRLLYIICSQAWDTIGSHYWIKQCIELLILTANTMMQIQCSNEQFKIPSITSVIPVNSSETQENSFVSFLSSHSESFDIIQTVDDKDDVYDIDLNADRKEDCQQILPNRRVTLVELVYKQAEFLEANRNIRTDQMLVATSQLCHIDTQLAQSVWLSMFPRIWSIFTEDQRCNITKELIPFLSSGTNVNQKDCHPSTLNTFVESLTKCAPPIYIPPNLLAYLGKSHNLWHRAILVLEDMAVNQSMQSKDIDGGENQFSDLDVQQSNNIFDSLSKMYSSMHEEDLWAGLWLKFAHYPETNIAVSYEQMGFFEEAQGAYDLAMTKFKQDLSNGVVNTYVNSELLLWENHWMRCAKELNQWDILLDYAQTNKDKNMFLILESSWRVPDWNLMKIALAKTEQCYLKHYGFKINLYKGYLSILHQEERQTGNIERYVEIASSLCIREWRRLPNIVSHIHLPYLQASQQIMELHEASQIHQGLAQSRNNSLHDMKAIVKTWRNRLPIISDDLSHWSDIFTWRQHHYQIITQHLEQQSDQGSTMLGVHASAQAIISFGKIARKHNLTGVCQETLSRIYTIPSVPIVDCFQKIRQQVKCYLQMPSTSGKNEINEALEVIESTNLKYFTGEMNAEFYALKGLLLAQIGRSEEAGKSFSVAAQLHDGLTKAWAMWGDYMEQIFLKERKITLAVDALICYLQASRNQIESKTRKYIAKVLWFLSYDNNTKILISTLEKHVAGIPPSYWLPWIPQLLCCLEQFEGDVILNLLSQIGRLYPQAVYFPIRTLYLTLKIEQREKHKTAEQAVKSSCSNIDGTTLSFGRGASHGNIPSINPIKATPPMWRCSKVMQLQREVHPTILSSLEGIVDQMVWFRESWTEEVLRQLRQGLIKCYAIAFEKRDTVQHSTITPHTLHFVKKLGSTFGIGIENVPGSVTSSISNSAASESLARRAQVTFQDPVFQKMKEQFTNDFDFSKPGAMKLHNLISKLKTWIKVLETKVKKLPTSFLIEDKCRFLSNFSQKTAEVELPGELLIPLSSHYYVRIARFMPRVEIVQKNNTAARRLYIRGTNGKIYPYLVVLDSGLGDARREERVLQLKRMLNYYLEKQKETSRRFLNITVPRVVPISPQMRLAEDNPNSISLLKIFKKCCQSMQVDYDMPIVKYYDRLSEVQARGTPTTHTLLREIFSEIQWTMVPKTLLKHWALKTFLAATDFWHFRKMLTLQLALAFLCEHALNLTRLNADMMYLHQDSGLMNISYFKFDVNDDKCQLNQHRPVPFRLTPNVGEFITHFGITGPLSAAIVATARCFIQPNYKLSSILQTILRDEIIALQKKGFRECKLIEGSEDRYSDGNCMEHSVNIVNSAVDIIMTRFNKISYFDSIENKKISVLVQSATNIDNLCRMDPAWHPWL

Target ORF information:

RefSeq Version	NM_001103722.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nipped-A, transcript variant E (Nipped-A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103722.3

ORF Insert Sequence:

ATGTCGGTTA TTGAAAATGT ACCGGTGAAT ACATTTCGGA ATTATTTAAA TATTTTAAAT 
GATTCCTCTT CTAAAGACGA ACTTAAGCTG AAAGCTACTC AAGAGCTAAG TGAACACTTT 
GAAATGATAA TGCAAAGTCC GGCATATCCG TCGTTCTTAG ATAATTCTTT AAAAATATTT 
ATGCGGATAC TTCAGGATGG CGAACCTCAG TTCATTCAAG AAAATACAAT GCAACACATA 
CGTAAACTGA TTTTAGAGAT GATTCATCGA TTGCCTATAA CAGAAAGCTT AAGGCAACAT 
GTAAAGACAA TAATAACAAT GATGCTTAAA ATACTTAAAA CTGACAACGA AGAAAATGTT 
CTTGTATGTC TTCGCATTAT AATTGAACTG CATAAGCATT TTCGTCCATC ATTTAATTCT 
GAAATTCAAC TATTTTTGGG ATTTGTTAAG GAAATCTATA CAAATCTTCC TAACCATCTT 
ACATCTATAT TTGAAACATC GAATGATGTT TGGGTTACCG ATTTAAAGGA CTTAAATTTG 
GAAGTACTTT TATCCGAATC ATATTCTGTT AGAACTATCC ATGTCGAAAA GGCACTTGAT 
TCAAATTCTC AACAACAAAT TTATAATCTC CTTCCTCGTG GAATATTATC TTTGAAAGTT 
TTACAAGAGC TCCCAATAAT AGTAGTACTA ATGTACCAAA TATATAAAAA CGCAGTACAT 
CAAGAAGTTT CTGAATTCAT TCCTCTTATA TTGACTACTA TTAATTTGCA ACCAACAGTA 
ACACGAAGAA ATTCTCCTCA GAAAGAAATA TATGTCGAAT TCATGGGTGC ACAAATTAAA 
ACTCTGTCAT TTTTGGCGTA CATTGTCCGA ATATTTCAGG AAGTGGTAAT AGCTAGTTCA 
TTATCTGTAA CCAGCGGAAT GCTCAACCTT ATGAAAAATT GTCCCAAGGA AGCCGCTCAT 
CTCAGAAAAG AACTTTTAAT TGCTGCCCGT CATATATTTG CTACAGATTT ACGTCAAAAA 
TTTATTCCAT CAATCGAACA ATTGTTTGAT GAGGATTTGC TTATTGGAAA AGGAGTTACA 
TTGGATTCTA TTCGGCCATT GGCGTATTCA ACTTTAGCTG ACCTAGCTCA CCACGTTCGC 
CAAAGCCTAA ATATAGACGT TTTAATAAAG GCAGTGAATC TCTTTTCAAA AAATGTTCAT 
GATGAATCAC TTGCTGTGGG AATACAAACT ATGTCATGCA AGCTTTTGTT AAATCTAGTT 
GATTGTTTAC GTCATCACAG TGAAACAGAA CCACAAAGAT CAAAAGCTCT ACTCTCAAAA 
CTGTTAAAAG TGTTTGTAAA AAAATTTGAA ACTATAGCTA AAATTCAGCT TCCATTAATA 
ATTCAAAAGT GCAAAGGGCA CGCATTTTCT GGAGCATTGG TAAATTCATC TGGCAACGCT 
TCCTTATCAC ACATTAACGC GCCAGATCTA AAAGACGACA TATCCAACAT CCAAGTATCT 
GCATCTGGAT CACAATGGAT ATATTCCGTC AATGTCGCTG AATTTCGAAG CTTAGTAAAA 
ACCCTTGTTG GTGGAGTAAA AACAATAACT TGGGGTTTTT TTAACTCAAA GTTTCAGCTA 
ACTGATACCA AACTGGCCAA CCATGAAAAA ATTTTTGGAC CTGAAATCGT ATGTTCTTAC 
ATCGACTTGG TTTACTATGC GATGGAGGCT CTAGATATAT ACACTATAAA CGTTAATCCA 
AATCAACAGA GAACGTCTGG GTTGATTTCC AGAAGTAAAG AAGAAAAAGA AGTCTTGGAG 
CATTTTAGTG GCATATTTTT AATGATGCAC TCCCAAAACT TTCAAGAAAT ATTTTCTACT 
ACGATTAATT TTTTGGTGGA AAGGATCTAC AAAAATCAGT CCTTACAAGT GATAGCGAAC 
TCCTTTTTAG CAAATCCTAC TACTTCACCT CTGTTTGCCA CTGTTCTAGT GGAATATCTT 
TTGAACAAAA TGGAAGAAAT GGGATCAAAC TTGGAACGCT CCAACTTGTA TCTACGATTA 
TTCAAGTTGG TATTCGGCAG CGTAAGTCTT TTTCCAGTCG AAAATGAACA GATGTTGCGT 
CCACATCTTC ATAAAATAGT CAATAGATCC ATGGAATTGG CTTTGATATC GGAGGAGCCG 
TATAACTATT TTTTACTTCT GAGGGCATTG TTTAGATCGA TTGGAGGAGG AAGCCATGAC 
CTATTATACC AAGAGTTTCT GCCACTTTTA CCAAACTTAT TAGAAGGATT AAATCGCCTA 
CAGAGTGGAT TTCATAAGCA GCACATGCGA GATCTTTTCG TGGAACTTTG CCTTACAGTT 
CCGGTCCGCT TGTCATCTTT ACTGCCCTAT TTACCAATGT TGATGGACCC ATTAGTTTCT 
GCGTTAAACG GATCTCCAAC ATTAATAAGT CAGGGCCTGC GAACCTTAGA GCTTTGCGTT 
GACAATTTGC AGCCAGACTT TTTATATGAT CATATACAAC CAGTAAGAGC TGCTTTAATG 
CAAGCATTGT GGAAAACTTT AAGAAATCAG GATAACGCCG CTTTGGTTGC CTTTCGCGTT 
CTAGGAAAAT TTGGTGGCGG CAATCGAAAA ATGATGGTGG AGCCACAAGC TTTGTCGTAT 
ATTATAAACG ACAAGCCCAC CATTTCCATT GTAACTTATT TTCAGGAATA CGAAACACCA 
ATAGATTTTC CTGTTGATGA AGCGATTAAG TCCGCCTTTA GAGCATTGGG ATCAAATTCT 
ACTGACCAGT TTTATAGGAG GCAAAGCTGG GAAGTAATCA GGTGCTTCTT AGCTGCTTTT 
ATTAGTTTGG ATGATGAAAA ACATATGCTG CTGAAACTTT TTACACATGT AGATTTTGTT 
GAAAATAAAA TTATGAATTG GTCTACGTTT CAACATAAAG CCGGGAACGA AACTGTACGA 
GAAACTCATC AGACAGCCCT CATAGGTATG TTGGTTGCAT CAGCAACAAA GGACTTGCGT 
GATTCAGTGT GCCCAGTAAT GGCCGCTGTT GTGAGGCATT ATACTATGGT AGCTATTGCA 
CAGCAGGCCG GACCTTTTCC ACAAAAAGGC TACCAAGCCA CTCATGGAAT TGACCCCATG 
ATATTAATAG ATGCTTTAGC CTCTTGCATG GGTCATGAAG AAAAGGAGTT ATGCAAACCC 
GGAATAGCTT GTATGGGTAT TATACTTGAC ACTGCAACAA ATATTATGGG TAATAAAGAC 
AGAGCGTGTA AATTGCCCAT AATACAGTAT CTAGCAGAAA AAATGGTATC CTTATGCTAT 
GATCGGCCAT GGTACTCAAA AGTAGGCGGT TGCCAAGCAA TTCAATTTTT ATGCAAACAT 
ATGTCTTTGC GAGCCCTTTT TCAAAATCTC TTTAATTTTT TAAAAGCTTT CATGTTTGTA 
TTAATGGACC TAGAGGGAGA CGTTTCTAAT GGGGCTATTG AAATAACAAA AAGTTATATG 
AAAAGCATGT TGGAAATTTG TTTAACGCCA ATAAATGAAT GTTATAAGAA TATAGATCTA 
AAGGACTTAC AAGCCAAAGC CACTTATGAA GTTATTCACG AATTAGTGCG GCACATCACA 
AGCCCTAATA CCATTGTTAG AGAAGAATCT ATGGTACTAC TTAAACATAT TGGTACAATA 
CAATCCAAAA CCGTTTCTGA AGTTATGGAT CCTCATAAAG ACGTTCTCGC TGACATTATA 
CCGCCAAAGA AGCATTTATT GAGACATCAA CCCGCAAATG CGCAAATCGG ACTTATGGAT 
GGCAATACAT TCTGCACAAC TTTGGAGCCA AGACTTTTTA CAATTGATTT GACGAATACC 
TACCACAAAT TATTTTTTCA CGAGTTACTT ACACTAAGTG AAGCCGAAGA TGCCACTTTA 
GCAAAGTTGG ACTGTTACAA AAACGTTCCG AATCTTATAC CACTTAGGAC TAGTGCGTTG 
CGAGCTTTAG CAGCATGCCA CTATATAAGT GATATCGGAT ACAAAGAAAA AATAATAAAT 
ATAATTTTTA AAGTAATGGA AAGTGATAAA TCAGAACTTC AGACAACTGC GTTTCATTGC 
ATGAAGCATT TTATAACTGG AGTTACTCTG GAAAAAGAAA AGGTACAATC TGCAATGCGC 
CCACTGCTAT TAAAATTGGG CGACCACAGA AATTTATCGA TACCAGCAAT AAAACGCTTA 
TCTTACTTTA CTCAGATCTT TCCTCAAATG TTCAATGAGA AGTTAAGTGA GCAAATTTTG 
CAACATTGCT CTAAAATTAT GGAAATATTT GTTAGTGAAT ACAAATCCAC AAGTCCAAAC 
GTTAATTTTT TTGCATCATC GAAAGGTGGA GAATATGAAC AGAAAATTGT CATACTAATC 
GAAATGTTTT TCTATATATC AGCATCAGTA AAATACATTG AAAAGCTGTG CCAACTTGTT 
TTAAAAACGG AAAAAAACTT AATGATTGAG GCATCGAGTC CATATCGAGA AGCCCTAATT 
AAGTTTCTTC AACGATTTCC AACAGAAACT GTGGATTTAT TTTTAACGGA ATCTCTTATG 
ATAGATCCGC AATGGAATCG TCTTTTTATA TACTTGTTGA AACACGAAAC GGGAGTATCA 
TTTCGAGCAG TTATTAAAAG TAGTCGGTAT AATAATTTAA TACATTATTT AAATACACAC 
ACAGAATTTC CAGAAGCACT CAAATATGAA ATTCAGCACC AAGCCGTTTT AATAATTTTT 
ACATTAATGG AATCAGACGA TCAATGGATA CCAACACGAC AAGATATTGT TGACGCCCTT 
AAAAATTGTT GGCAGAATTA TTTAAGTACA TTAAGCTCTG AAGACGTTCT GTGTGACTTA 
TGGCATTTGA TTGGAAAAAT ACTTTTGCAT TATTTTTCTA ACAATACAAA TGACATTGAA 
CTGCTGTTTC AATTATTACG TGCTTTATGC TTCCGATTTA TACCGGATGT CTATTTCCTG 
CGGGACTTCT TGCAACACAC AGTAGCACAA AGTTTTACGG TTAACTGGAA ACGAAACGCA 
TTCTTTTACT TTGTTGAAAA CTTTAATAAC AGCTTTCTTT CAGAAGAGCT AAAGGCAAAA 
ATAATAACAG CAGTCATAAT ACCCTGTTTT GCTGTAAGTT TCGATAAAGG TGAAGGAAAT 
AAACTAATTG GGGCTCCTCC GACCCCGTAT CAAGAAGATG AAAAAAATAT TGTGTCCGTT 
TTTATTAATA AAGTGTTTGA CCCAGATAAA CAGTATGATG ATGCAGTAAG AATTGCTCTA 
TTACAACTAG CATGCTTATT GGTAGAACGT GCATCTCAGC ACATACATGA CGGAGATGCT 
AATAATAAGC GCCAAGGCAA TAAGCTTAGA CGACTCATGA CCTTTGCCTG GCCCTGTTTG 
CTGAGTAAAA GTTCAGTGGA TCCTACTGCA CGATACCACG GTCACTTATT ACTTTCGCAT 
ATAATCGCAA GATTGGCCAT TCACAAGAAA ATAGTTTTAC AAGTATTTCA TTCCTTGTTG 
AAAGGACACG CATTAGAGGC CAGATCTATT GTTAAACAGG CACTTGATGT TCTAACGCCA 
GCTATGCCGT TGCGAATGGA GGATGGAAAT ACTATGCTAA CACACTGGAC AAAAAAAATT 
ATAGTTGAAG AAGGTCACGC TATGCAGCAG TTATTTCATA TTTTGCAGCT CATTATTCGT 
CATTATAAAG TGTATTTTCC GGTTCGACAT CAACTAGTGC AACACTTGAT TAACTATATG 
CAGCGCCTTG GGTTTCCTCC TACTGCATCT ATTGAACATA AAAAACTTGC TGTGGACTTA 
GCAGAAGTTA TTATAAAATG GGAACTTCAT CGTATTAAGG ATGATCGTGA AACTAAAACA 
GATGGAACCG AAGAGGAGTT AATACAGGAA AGCTCAGTAA AACGATCAGG GATCGATTTA 
GTAGAAACTC GAAAAAAGTC ATTCGATATT ATAAGAGAAA CAACAGTCCA AGGAGTGGGA 
TCACATACCA AGCCTGATGA CATACTGCGA TCAATAGATA AATCCTATTG TGATACGGTT 
TTAAATTTCT TGATACGTTT AGCATGTCAG GTTAATGACC CGCAAGCCCC AATACTTTCG 
CCAGGAGAAA GTTTGTCTCG AAGGTGCGTT ATGCTATTAA AAATGGCGAT GCGTCCCGAG 
ATCTGGCCTC AACCATTTGA TATTAAGCTT AATTGGCTTG ATAAAGTTCT TGCTACAGTG 
GAAACCCCTC ATCACAACTT AAACAACATA TGTACTGGAA TAGATTTTTT AACATTTTTA 
ACTACTATAC TAAGTCCAGA TCAGTTGGTG TCAATTATAC GACCGGTTCA ACGAGGCCTG 
TCCTTGTGTA TAATTCATCA AAATACACGA ATTGTGCGGT TAATGCATAT GTTTTTGACG 
CGAATAATGG CAATTTTTCC ACCTGACACC CAACACAAGC ATGAAGATCT TGATTTGTTA 
TATACCGCTG TTAGTAAAAT GATCGCTGAA AACCTAACAA GTTACGAAAA GAGTCCACAA 
CCGAATGCTT CTTCGCTTTT TGGTACCTTA ATGATTTTGA AAGCGTGCAC TACTAATAAT 
GCAAGTTATA TTGATCGAAT TTTAGTCCAG TTTATAAGGG TTCTGAATCA TCTTACAAGG 
GATCATATTA ACACCATTGG TGGTAACACT GTTATTAGCC AATCTCCTGA TTCAAATGCT 
TTACCTTTAG AACTCTTAGT TCTCTCTTTG GAATTAATCA AGAATAGGAT CTTTGTAATG 
AGTGTGGAAA TCAGAAAACT TTTCATAGGC ACCATTTTGG TTAGTCTTAT AGAAAAGAGC 
ACGGAGGTAA AAATTATAAA ATGTATAATT AAAATGCTGG ATGAATGGAT TAAAACGAAA 
GAGCCAAATG TTATGACACA AGTTCCTTCT ATTCGCGAAA AGTCGGCCTT GCTGGTAAAG 
TTAATGCAAA ACGTTGAAAA GAAATTTACT GATGAAATAG AGCTTAATAT ACAATTCTTG 
GAAATCATAA ATTTTATATA TAGAGATGAA ATTCTTAAAC AAACTGAGTT AACAAACAAA 
CTAGAGGGAG CTTTTTTAAA CGGATTACGT TTTCAAAATC CAAACGTACG CTCGAAATTT 
TTTGAGATTT TAGACTCATC AATGCGGCGT AGACTTCACG ATCGATTGTT ATATATAATT 
TGTTCCCAAG CTTGGGACAC AATTGGTTCC CATTATTGGA TAAAACAATG CATTGAATTG 
CTTATTTTAA CAGCCAATAC AATGATGCAA ATTCAATGTT CAAATGAACA ATTTAAAATA 
CCCAGCATTA CTTCAGTCAT TCCAGTGAAT TCATCAGAAA CACAGGAAAA TTCCTTTGTA 
TCCTTCTTAT CCTCTCATTC CGAATCTTTT GACATTATAC AAACTGTTGA TGATAAAGAC 
GACGTGTATG ATATCGATTT AAATGCTGAT CGCAAAGAAG ATTGTCAACA AATACTACCA 
AATCGACGTG TTACTCTTGT TGAACTAGTT TACAAGCAAG CTGAATTTTT AGAAGCAAAC 
CGAAATATTA GGACCGACCA GATGCTTGTC GCCACATCTC AGCTATGTCA TATTGATACA 
CAGTTAGCTC AAAGCGTATG GTTATCTATG TTTCCACGTA TTTGGAGTAT ATTCACTGAA 
GATCAAAGGT GTAATATCAC AAAAGAACTG ATTCCCTTTT TATCGTCTGG AACTAACGTT 
AATCAAAAAG ACTGCCATCC AAGTACATTA AATACTTTTG TAGAGAGTTT AACTAAATGT 
GCGCCACCCA TATATATTCC ACCTAATTTA TTAGCATACC TAGGCAAATC TCATAACCTA 
TGGCATAGAG CTATACTTGT TTTAGAAGAT ATGGCCGTTA ATCAATCAAT GCAATCCAAG 
GATATTGATG GCGGTGAAAA TCAATTCTCT GACTTGGATG TACAACAATC AAATAATATA 
TTTGATTCAC TTTCAAAAAT GTATTCTTCG ATGCATGAGG AAGATCTTTG GGCTGGCCTA 
TGGCTTAAAT TTGCACACTA CCCGGAAACA AATATAGCTG TTTCGTATGA GCAAATGGGA 
TTTTTCGAAG AAGCCCAAGG TGCCTATGAT CTAGCAATGA CCAAATTTAA ACAAGATCTA 
AGTAATGGTG TAGTTAATAC ATATGTTAAT AGTGAATTAT TATTGTGGGA AAATCACTGG 
ATGCGATGTG CTAAAGAATT GAACCAATGG GACATTTTAC TGGACTATGC CCAAACTAAT 
AAGGACAAAA ATATGTTTTT GATTCTGGAA AGTTCGTGGC GCGTACCTGA TTGGAATTTG 
ATGAAAATCG CACTGGCTAA AACAGAACAA TGCTATTTAA AACACTACGG CTTTAAAATC 
AACCTTTACA AAGGGTATTT GAGTATTCTC CACCAAGAAG AAAGGCAAAC AGGCAATATC 
GAACGATATG TTGAAATTGC ATCCAGCTTA TGCATTCGTG AATGGCGTCG ATTGCCGAAC 
ATAGTTTCAC ATATTCATTT GCCATATCTT CAAGCATCAC AACAAATTAT GGAGCTTCAT 
GAAGCAAGTC AAATCCATCA GGGACTTGCT CAATCGCGCA ACAATTCACT TCACGATATG 
AAAGCTATCG TGAAAACTTG GCGTAATCGT TTACCTATTA TTTCTGATGA CTTATCGCAT 
TGGAGTGACA TATTTACATG GAGACAACAT CACTACCAAA TAATAACACA ACACCTAGAA 
CAACAATCGG ATCAAGGAAG TACAATGCTA GGAGTTCACG CATCAGCACA AGCTATAATT 
TCTTTTGGAA AAATAGCTCG GAAACACAAT TTGACTGGTG TTTGTCAGGA GACGTTGTCC 
AGGATATATA CAATTCCGTC TGTTCCTATC GTGGATTGTT TTCAGAAAAT TCGGCAGCAA 
GTAAAATGCT ACCTGCAAAT GCCCTCAACA TCTGGAAAAA ATGAAATTAA TGAAGCCTTG 
GAAGTAATTG AGTCCACGAA TTTAAAATAC TTCACTGGTG AAATGAATGC TGAATTTTAC 
GCTCTAAAGG GGCTATTATT AGCACAAATT GGAAGATCAG AAGAGGCTGG AAAATCATTT 
AGTGTTGCTG CTCAGCTTCA TGATGGTCTT ACCAAAGCCT GGGCAATGTG GGGTGACTAT 
ATGGAACAAA TATTTTTAAA AGAAAGGAAA ATCACATTAG CCGTCGATGC TTTAATTTGT 
TATTTACAAG CAAGCAGAAA TCAAATTGAA AGCAAAACCC GAAAATATAT TGCAAAAGTT 
TTGTGGTTTC TGTCTTATGA TAATAATACT AAAATCCTCA TAAGCACTTT AGAAAAGCAT 
GTGGCAGGCA TTCCACCCTC TTATTGGCTA CCATGGATTC CTCAGTTGCT CTGTTGCTTA 
GAACAGTTCG AAGGGGATGT TATATTAAAT CTCTTAAGCC AAATTGGACG CCTTTATCCT 
CAAGCGGTAT ATTTCCCGAT TCGGACTTTA TATTTGACTT TAAAAATCGA ACAACGCGAA 
AAACATAAAA CTGCTGAACA GGCTGTAAAA AGTTCATGCT CGAACATCGA TGGAACTACT 
TTAAGCTTTG GAAGGGGAGC AAGTCACGGA AACATTCCAT CAATAAATCC CATTAAAGCA 
ACTCCGCCCA TGTGGCGCTG CTCTAAGGTG ATGCAATTAC AGAGAGAAGT ACATCCAACA 
ATATTAAGTT CATTGGAAGG AATTGTAGAC CAAATGGTTT GGTTTAGAGA AAGCTGGACA 
GAGGAAGTTC TTCGACAACT ACGCCAAGGC CTAATTAAAT GCTATGCCAT AGCCTTTGAA 
AAAAGGGATA CTGTTCAACA TTCTACCATA ACACCTCACA CGTTGCATTT TGTCAAAAAG 
CTGGGTTCTA CGTTTGGCAT TGGAATAGAA AATGTTCCGG GATCAGTAAC CTCCTCAATT 
TCTAATTCAG CAGCCTCGGA GTCTCTTGCT CGACGCGCCC AAGTTACTTT TCAAGATCCA 
GTATTTCAAA AAATGAAGGA GCAATTCACT AATGACTTCG ATTTTTCAAA ACCTGGTGCC 
ATGAAATTGC ACAACTTGAT ATCAAAATTA AAAACATGGA TAAAAGTCCT GGAGACTAAA 
GTTAAAAAAT TACCCACGTC CTTTTTGATA GAAGACAAGT GTAGGTTTTT ATCAAACTTT 
AGTCAGAAGA CAGCTGAGGT TGAACTTCCT GGAGAATTGT TAATTCCCTT ATCATCTCAT 
TATTATGTAA GAATCGCAAG ATTCATGCCG CGTGTGGAAA TTGTACAAAA AAATAACACA 
GCAGCGCGTA GGTTATATAT AAGAGGTACT AACGGGAAAA TCTATCCGTA CCTTGTAGTT 
CTTGATTCAG GTTTGGGAGA TGCTCGCCGA GAGGAAAGAG TTTTGCAGTT AAAACGCATG 
TTGAATTACT ATTTAGAAAA ACAAAAAGAG ACAAGTCGAA GATTTCTTAA CATAACGGTA 
CCAAGGGTTG TCCCGATATC GCCCCAAATG AGATTGGCAG AAGATAACCC AAACAGTATT 
TCATTGTTAA AAATATTTAA AAAATGCTGT CAAAGTATGC AGGTTGACTA CGACATGCCA 
ATAGTTAAGT ATTATGACCG TCTTTCTGAA GTACAGGCAA GAGGCACTCC AACTACACAT 
ACCCTATTGA GAGAAATATT CTCTGAAATT CAATGGACTA TGGTCCCAAA AACATTACTA 
AAGCATTGGG CTTTGAAAAC ATTCTTGGCG GCTACTGACT TTTGGCATTT CCGAAAAATG 
CTTACCCTGC AGTTGGCTTT GGCATTTTTA TGCGAACACG CTTTGAATCT TACTCGACTG 
AATGCGGATA TGATGTACCT TCATCAAGAC TCAGGACTTA TGAACATATC TTATTTTAAG 
TTTGATGTAA ATGATGATAA GTGCCAGCTT AATCAACACC GACCTGTACC ATTTCGCCTG 
ACTCCGAATG TTGGTGAATT CATAACACAT TTTGGAATAA CTGGACCTTT ATCTGCAGCA 
ATTGTGGCAA CGGCTCGGTG TTTTATTCAA CCAAATTACA AATTAAGCTC AATATTACAA 
ACCATTTTAA GAGATGAAAT AATAGCCCTG CAAAAAAAAG GATTCAGAGA ATGTAAACTA 
ATCGAAGGCT CTGAAGACCG TTATTCCGAT GGAAATTGTA TGGAGCACTC AGTAAACATT 
GTGAATTCAG CGGTGGATAT CATAATGACG CGTTTTAATA AAATATCTTA TTTTGATAGC 
ATTGAAAATA AGAAGATTTC CGTGCTCGTT CAATCGGCAA CTAACATTGA TAATCTTTGT 
CGTATGGATC CTGCTTGGCA TCCCTGGCTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nipped-A ( NM_001316406.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303335.1 Drosophila melanogaster Nipped-A, transcript variant F (Nipped-A), mRNA.

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CloneID	OFa41281
Clone ID Related Accession (Same CDS sequence)	NM_001316406.1
Accession Version	NM_001316406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nipped-A, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGTTA TTGAAAATGT ACCGGTGAAT ACATTTCGGA ATTATTTAAA TATTTTAAAT 
GATTCCTCTT CTAAAGACGA ACTTAAGCTG AAAGCTACTC AAGAGCTAAG TGAACACTTT 
GAAATGATAA TGCAAAGTCC GGCATATCCG TCGTTCTTAG ATAATTCTTT AAAAATATTT 
ATGCGGATAC TTCAGGATGG CGAACCTCAG TTCATTCAAG AAAATACAAT GCAACACATA 
CGTAAACTGA TTTTAGAGAT GATTCATCGA TTGCCTATAA CAGAAAGCTT AAGGCAACAT 
GTAAAGACAA TAATAACAAT GATGCTTAAA ATACTTAAAA CTGACAACGA AGAAAATGTT 
CTTGTATGTC TTCGCATTAT AATTGAACTG CATAAGCATT TTCGTCCATC ATTTAATTCT 
GAAATTCAAC TATTTTTGGG ATTTGTTAAG GAAATCTATA CAAATCTTCC TAACCATCTT 
ACATCTATAT TTGAAACATC GAATGATGTT TGGGTTACCG ATTTAAAGGA CTTAAATTTG 
GAAGTACTTT TATCCGAATC ATATTCTGTT AGAACTATCC ATGTCGAAAA GGCACTTGAT 
TCAAATTCTC AACAACAAAT TTATAATCTC CTTCCTCGTG GAATATTATC TTTGAAAGTT 
TTACAAGAGC TCCCAATAAT AGTAGTACTA ATGTACCAAA TATATAAAAA CGCAGTACAT 
CAAGAAGTTT CTGAATTCAT TCCTCTTATA TTGACTACTA TTAATTTGCA ACCAACAGTA 
ACACGAAGAA ATTCTCCTCA GAAAGAAATA TATGTCGAAT TCATGGGTGC ACAAATTAAA 
ACTCTGTCAT TTTTGGCGTA CATTGTCCGA ATATTTCAGG AAGTGGTAAT AGCTAGTTCA 
TTATCTGTAA CCAGCGGAAT GCTCAACCTT ATGAAAAATT GTCCCAAGGA AGCCGCTCAT 
CTCAGAAAAG AACTTTTAAT TGCTGCCCGT CATATATTTG CTACAGATTT ACGTCAAAAA 
TTTATTCCAT CAATCGAACA ATTGTTTGAT GAGGATTTGC TTATTGGAAA AGGAGTTACA 
TTGGATTCTA TTCGGCCATT GGCGTATTCA ACTTTAGCTG ACCTAGCTCA CCACGTTCGC 
CAAAGCCTAA ATATAGACGT TTTAATAAAG GCAGTGAATC TCTTTTCAAA AAATGTTCAT 
GATGAATCAC TTGCTGTGGG AATACAAACT ATGTCATGCA AGCTTTTGTT AAATCTAGTT 
GATTGTTTAC GTCATCACAG TGAAACAGAA CCACAAAGAT CAAAAGCTCT ACTCTCAAAA 
CTGTTAAAAG TGTTTGTAAA AAAATTTGAA ACTATAGCTA AAATTCAGCT TCCATTAATA 
ATTCAAAAGT GCAAAGGGCA CGCATTTTCT GGAGCATTGG TAAATTCATC TGGCAACGCT 
TCCTTATCAC ACATTAACGC GCCAGATCTA AAAGACGACA TATCCAACAT CCAAGTATCT 
GCATCTGGAT CACAATGGAT ATATTCCGTC AATGTCGCTG AATTTCGAAG CTTAGTAAAA 
ACCCTTGTTG GTGGAGTAAA AACAATAACT TGGGGTTTTT TTAACTCAAA GTTTCAGCTA 
ACTGATACCA AACTGGCCAA CCATGAAAAA ATTTTTGGAC CTGAAATCGT ATGTTCTTAC 
ATCGACTTGG TTTACTATGC GATGGAGGCT CTAGATATAT ACACTATAAA CGTTAATCCA 
AATCAACAGA GAACGTCTGG GTTGATTTCC AGAAGTAAAG AAGAAAAAGA AGTCTTGGAG 
CATTTTAGTG GCATATTTTT AATGATGCAC TCCCAAAACT TTCAAGAAAT ATTTTCTACT 
ACGATTAATT TTTTGGTGGA AAGGATCTAC AAAAATCAGT CCTTACAAGT GATAGCGAAC 
TCCTTTTTAG CAAATCCTAC TACTTCACCT CTGTTTGCCA CTGTTCTAGT GGAATATCTT 
TTGAACAAAA TGGAAGAAAT GGGATCAAAC TTGGAACGCT CCAACTTGTA TCTACGATTA 
TTCAAGTTGG TATTCGGCAG CGTAAGTCTT TTTCCAGTCG AAAATGAACA GATGTTGCGT 
CCACATCTTC ATAAAATAGT CAATAGATCC ATGGAATTGG CTTTGATATC GGAGGAGCCG 
TATAACTATT TTTTACTTCT GAGGGCATTG TTTAGATCGA TTGGAGGAGG AAGCCATGAC 
CTATTATACC AAGAGTTTCT GCCACTTTTA CCAAACTTAT TAGAAGGATT AAATCGCCTA 
CAGAGTGGAT TTCATAAGCA GCACATGCGA GATCTTTTCG TGGAACTTTG CCTTACAGTT 
CCGGTCCGCT TGTCATCTTT ACTGCCCTAT TTACCAATGT TGATGGACCC ATTAGTTTCT 
GCGTTAAACG GATCTCCAAC ATTAATAAGT CAGGGCCTGC GAACCTTAGA GCTTTGCGTT 
GACAATTTGC AGCCAGACTT TTTATATGAT CATATACAAC CAGTAAGAGC TGCTTTAATG 
CAAGCATTGT GGAAAACTTT AAGAAATCAG GATAACGCCG CTTTGGTTGC CTTTCGCGTT 
CTAGGAAAAT TTGGTGGCGG CAATCGAAAA ATGATGGTGG AGCCACAAGC TTTGTCGTAT 
ATTATAAACG ACAAGCCCAC CATTTCCATT GTAACTTATT TTCAGGAATA CGAAACACCA 
ATAGATTTTC CTGTTGATGA AGCGATTAAG TCCGCCTTTA GAGCATTGGG ATCAAATTCT 
ACTGACCAGT TTTATAGGAG GCAAAGCTGG GAAGTAATCA GGTGCTTCTT AGCTGCTTTT 
ATTAGTTTGG ATGATGAAAA ACATATGCTG CTGAAACTTT TTACACATGT AGATTTTGTT 
GAAAATAAAA TTATGAATTG GTCTACGTTT CAACATAAAG CCGGGAACGA AACTGTACGA 
GAAACTCATC AGACAGCCCT CATAGGTATG TTGGTTGCAT CAGCAACAAA GGACTTGCGT 
GATTCAGTGT GCCCAGTAAT GGCCGCTGTT GTGAGGCATT ATACTATGGT AGCTATTGCA 
CAGCAGGCCG GACCTTTTCC ACAAAAAGGC TACCAAGCCA CTCATGGAAT TGACCCCATG 
ATATTAATAG ATGCTTTAGC CTCTTGCATG GGTCATGAAG AAAAGGAGTT ATGCAAACCC 
GGAATAGCTT GTATGGGTAT TATACTTGAC ACTGCAACAA ATATTATGGG TAATAAAGAC 
AGAGCGTGTA AATTGCCCAT AATACAGTAT CTAGCAGAAA AAATGGTATC CTTATGCTAT 
GATCGGCCAT GGTACTCAAA AGTAGGCGGT TGCCAAGCAA TTCAATTTTT ATGCAAACAT 
ATGTCTTTGC GAGCCCTTTT TCAAAATCTC TTTAATTTTT TAAAAGCTTT CATGTTTGTA 
TTAATGGACC TAGAGGGAGA CGTTTCTAAT GGGGCTATTG AAATAACAAA AAGTTATATG 
AAAAGCATGT TGGAAATTTG TTTAACGCCA ATAAATGAAT GTTATAAGAA TATAGATCTA 
AAGGACTTAC AAGCCAAAGC CACTTATGAA GTTATTCACG AATTAGTGCG GCACATCACA 
AGCCCTAATA CCATTGTTAG AGAAGAATCT ATGGTACTAC TTAAACATAT TGGTACAATA 
CAATCCAAAA CCGTTTCTGA AGTTATGGAT CCTCATAAAG ACGTTCTCGC TGACATTATA 
CCGCCAAAGA AGCATTTATT GAGACATCAA CCCGCAAATG CGCAAATCGG ACTTATGGAT 
GGCAATACAT TCTGCACAAC TTTGGAGCCA AGACTTTTTA CAATTGATTT GACGAATACC 
TACCACAAAT TATTTTTTCA CGAGTTACTT ACACTAAGTG AAGCCGAAGA TGCCACTTTA 
GCAAAGTTGG ACTGTTACAA AAACGTTCCG AATCTTATAC CACTTAGGAC TAGTGCGTTG 
CGAGCTTTAG CAGCATGCCA CTATATAAGT GATATCGGAT ACAAAGAAAA AATAATAAAT 
ATAATTTTTA AAGTAATGGA AAGTGATAAA TCAGAACTTC AGACAACTGC GTTTCATTGC 
ATGAAGCATT TTATAACTGG AGTTACTCTG GAAAAAGAAA AGGTACAATC TGCAATGCGC 
CCACTGCTAT TAAAATTGGG CGACCACAGA AATTTATCGA TACCAGCAAT AAAACGCTTA 
TCTTACTTTA CTCAGATCTT TCCTCAAATG TTCAATGAGA AGTTAAGTGA GCAAATTTTG 
CAACATTGCT CTAAAATTAT GGAAATATTT GTTAGTGAAT ACAAATCCAC AAGTCCAAAC 
GTTAATTTTT TTGCATCATC GAAAGGTGGA GAATATGAAC AGAAAATTGT CATACTAATC 
GAAATGTTTT TCTATATATC AGCATCAGTA AAATACATTG AAAAGCTGTG CCAACTTGTT 
TTAAAAACGG AAAAAAACTT AATGATTGAG GCATCGAGTC CATATCGAGA AGCCCTAATT 
AAGTTTCTTC AACGATTTCC AACAGAAACT GTGGATTTAT TTTTAACGGA ATCTCTTATG 
ATAGATCCGC AATGGAATCG TCTTTTTATA TACTTGTTGA AACACGAAAC GGGAGTATCA 
TTTCGAGCAG TTATTAAAAG TAGTCGGTAT AATAATTTAA TACATTATTT AAATACACAC 
ACAGAATTTC CAGAAGCACT CAAATATGAA ATTCAGCACC AAGCCGTTTT AATAATTTTT 
ACATTAATGG AATCAGACGA TCAATGGATA CCAACACGAC AAGATATTGT TGACGCCCTT 
AAAAATTGTT GGCAGAATTA TTTAAGTACA TTAAGCTCTG AAGACGTTCT GTGTGACTTA 
TGGCATTTGA TTGGAAAAAT ACTTTTGCAT TATTTTTCTA ACAATACAAA TGACATTGAA 
CTGCTGTTTC AATTATTACG TGCTTTATGC TTCCGATTTA TACCGGATGT CTATTTCCTG 
CGGGACTTCT TGCAACACAC AGTAGCACAA AGTTTTACGG TTAACTGGAA ACGAAACGCA 
TTCTTTTACT TTGTTGAAAA CTTTAATAAC AGCTTTCTTT CAGAAGAGCT AAAGGCAAAA 
ATAATAACAG CAGTCATAAT ACCCTGTTTT GCTGTAAGTT TCGATAAAGG TGAAGGAAAT 
AAACTAATTG GGGCTCCTCC GACCCCGTAT CAAGAAGATG AAAAAAATAT TGTGTCCGTT 
TTTATTAATA AAGTGTTTGA CCCAGATAAA CAGTATGATG ATGCAGTAAG AATTGCTCTA 
TTACAACTAG CATGCTTATT GGTAGAACGT GCATCTCAGC ACATACATGA CGGAGATGCT 
AATAATAAGC GCCAAGGCAA TAAGCTTAGA CGACTCATGA CCTTTGCCTG GCCCTGTTTG 
CTGAGTAAAA GTTCAGTGGA TCCTACTGCA CGATACCACG GTCACTTATT ACTTTCGCAT 
ATAATCGCAA GATTGGCCAT TCACAAGAAA ATAGTTTTAC AAGTATTTCA TTCCTTGTTG 
AAAGGACACG CATTAGAGGC CAGATCTATT GTTAAACAGG CACTTGATGT TCTAACGCCA 
GCTATGCCGT TGCGAATGGA GGATGGAAAT ACTATGCTAA CACACTGGAC AAAAAAAATT 
ATAGTTGAAG AAGGTCACGC TATGCAGCAG TTATTTCATA TTTTGCAGCT CATTATTCGT 
CATTATAAAG TGTATTTTCC GGTTCGACAT CAACTAGTGC AACACTTGAT TAACTATATG 
CAGCGCCTTG GGTTTCCTCC TACTGCATCT ATTGAACATA AAAAACTTGC TGTGGACTTA 
GCAGAAGTTA TTATAAAATG GGAACTTCAT CGTATTAAGG ATGATCGTGA AACTAAAACA 
GATGGAACCG AAGAGGAGTT AATACAGGAA AGCTCAGTAA AACGATCAGG GATCGATTTA 
GTAGAAACTC GAAAAAAGTC ATTCGATATT ATAAGAGAAA CAACAGTCCA AGGAAAGTGC 
GTTATGCTAT TAAAAATGGC GATGCGTCCC GAGATCTGGC CTCAACCATT TGATATTAAG 
CTTAATTGGC TTGATAAAGT TCTTGCTACA GTGGAAACCC CTCATCACAA CTTAAACAAC 
ATATGTACTG GAATAGATTT TTTAACATTT TTAACTACTA TACTAAGTCC AGATCAGTTG 
GTGTCAATTA TACGACCGGT TCAACGAGGC CTGTCCTTGT GTATAATTCA TCAAAATACA 
CGAATTGTGC GGTTAATGCA TATGTTTTTG ACGCGAATAA TGGCAATTTT TCCACCTGAC 
ACCCAACACA AGCATGAAGA TCTTGATTTG TTATATACCG CTGTTAGTAA AATGATCGCT 
GAAAACCTAA CAAGTTACGA AAAGAGTCCA CAACCGAATG CTTCTTCGCT TTTTGGTACC 
TTAATGATTT TGAAAGCGTG CACTACTAAT AATGCAAGTT ATATTGATCG AATTTTAGTC 
CAGTTTATAA GGGTTCTGAA TCATCTTACA AGGGATCATA TTAACACCAT TGGTGGTAAC 
ACTGTTATTA GCCAATCTCC TGATTCAAAT GCTTTACCTT TAGAACTCTT AGTTCTCTCT 
TTGGAATTAA TCAAGAATAG GATCTTTGTA ATGAGTGTGG AAATCAGAAA ACTTTTCATA 
GGCACCATTT TGGTTAGTCT TATAGAAAAG AGCACGGAGG TAAAAATTAT AAAATGTATA 
ATTAAAATGC TGGATGAATG GATTAAAACG AAAGAGCCAA ATGTTATGAC ACAAGTTCCT 
TCTATTCGCG AAAAGTCGGC CTTGCTGGTA AAGTTAATGC AAAACGTTGA AAAGAAATTT 
ACTGATGAAA TAGAGCTTAA TATACAATTC TTGGAAATCA TAAATTTTAT ATATAGAGAT 
GAAATTCTTA AACAAACTGA GTTAACAAAC AAACTAGAGG GAGCTTTTTT AAACGGATTA 
CGTTTTCAAA ATCCAAACGT ACGCTCGAAA TTTTTTGAGA TTTTAGACTC ATCAATGCGG 
CGTAGACTTC ACGATCGATT GTTATATATA ATTTGTTCCC AAGCTTGGGA CACAATTGGT 
TCCCATTATT GGATAAAACA ATGCATTGAA TTGCTTATTT TAACAGCCAA TACAATGATG 
CAAATTCAAT GTTCAAATGA ACAATTTAAA ATACCCAGCA TTACTTCAGT CATTCCAGTG 
AATTCATCAG AAACACAGGA AAATTCCTTT GTATCCTTCT TATCCTCTCA TTCCGAATCT 
TTTGACATTA TACAAACTGT TGATGATAAA GACGACGTGT ATGATATCGA TTTAAATGCT 
GATCGCAAAG AAGATTGTCA ACAAATACTA CCAAATCGAC GTGTTACTCT TGTTGAACTA 
GTTTACAAGC AAGCTGAATT TTTAGAAGCA AACCGAAATA TTAGGACCGA CCAGATGCTT 
GTCGCCACAT CTCAGCTATG TCATATTGAT ACACAGTTAG CTCAAAGCGT ATGGTTATCT 
ATGTTTCCAC GTATTTGGAG TATATTCACT GAAGATCAAA GGTGTAATAT CACAAAAGAA 
CTGATTCCCT TTTTATCGTC TGGAACTAAC GTTAATCAAA AAGACTGCCA TCCAAGTACA 
TTAAATACTT TTGTAGAGAG TTTAACTAAA TGTGCGCCAC CCATATATAT TCCACCTAAT 
TTATTAGCAT ACCTAGGCAA ATCTCATAAC CTATGGCATA GAGCTATACT TGTTTTAGAA 
GATATGGCCG TTAATCAATC AATGCAATCC AAGGATATTG ATGGCGGTGA AAATCAATTC 
TCTGACTTGG ATGTACAACA ATCAAATAAT ATATTTGATT CACTTTCAAA AATGTATTCT 
TCGATGCATG AGGAAGATCT TTGGGCTGGC CTATGGCTTA AATTTGCACA CTACCCGGAA 
ACAAATATAG CTGTTTCGTA TGAGCAAATG GGATTTTTCG AAGAAGCCCA AGGTGCCTAT 
GATCTAGCAA TGACCAAATT TAAACAAGAT CTAAGTAATG GTGTAGTTAA TACATATGTT 
AATAGTGAAT TATTATTGTG GGAAAATCAC TGGATGCGAT GTGCTAAAGA ATTGAACCAA 
TGGGACATTT TACTGGACTA TGCCCAAACT AATAAGGACA AAAATATGTT TTTGATTCTG 
GAAAGTTCGT GGCGCGTACC TGATTGGAAT TTGATGAAAA TCGCACTGGC TAAAACAGAA 
CAATGCTATT TAAAACACTA CGGCTTTAAA ATCAACCTTT ACAAAGGGTA TTTGAGTATT 
CTCCACCAAG AAGAAAGGCA AACAGGCAAT ATCGAACGAT ATGTTGAAAT TGCATCCAGC 
TTATGCATTC GTGAATGGCG TCGATTGCCG AACATAGTTT CACATATTCA TTTGCCATAT 
CTTCAAGCAT CACAACAAAT TATGGAGCTT CATGAAGCAA GTCAAATCCA TCAGGGACTT 
GCTCAATCGC GCAACAATTC ACTTCACGAT ATGAAAGCTA TCGTGAAAAC TTGGCGTAAT 
CGTTTACCTA TTATTTCTGA TGACTTATCG CATTGGAGTG ACATATTTAC ATGGAGACAA 
CATCACTACC AAATAATAAC ACAACACCTA GAACAACAAT CGGATCAAGG AAGTACAATG 
CTAGGAGTTC ACGCATCAGC ACAAGCTATA ATTTCTTTTG GAAAAATAGC TCGGAAACAC 
AATTTGACTG GTGTTTGTCA GGAGACGTTG TCCAGGATAT ATACAATTCC GTCTGTTCCT 
ATCGTGGATT GTTTTCAGAA AATTCGGCAG CAAGTAAAAT GCTACCTGCA AATGCCCTCA 
ACATCTGGAA AAAATGAAAT TAATGAAGCC TTGGAAGTAA TTGAGTCCAC GAATTTAAAA 
TACTTCACTG GTGAAATGAA TGCTGAATTT TACGCTCTAA AGGGGCTATT ATTAGCACAA 
ATTGGAAGAT CAGAAGAGGC TGGAAAATCA TTTAGTGTTG CTGCTCAGCT TCATGATGGT 
CTTACCAAAG CCTGGGCAAT GTGGGGTGAC TATATGGAAC AAATATTTTT AAAAGAAAGG 
AAAATCACAT TAGCCGTCGA TGCTTTAATT TGTTATTTAC AAGCAAGCAG AAATCAAATT 
GAAAGCAAAA CCCGAAAATA TATTGCAAAA GTTTTGTGGT TTCTGTCTTA TGATAATAAT 
ACTAAAATCC TCATAAGCAC TTTAGAAAAG CATGTGGCAG GCATTCCACC CTCTTATTGG 
CTACCATGGA TTCCTCAGTT GCTCTGTTGC TTAGAACAGT TCGAAGGGGA TGTTATATTA 
AATCTCTTAA GCCAAATTGG ACGCCTTTAT CCTCAAGCGG TATATTTCCC GATTCGGACT 
TTATATTTGA CTTTAAAAAT CGAACAACGC GAAAAACATA AAACTGCTGA ACAGGCTGTA 
AAAAGTTCAT GCTCGAACAT CGATGGAACT ACTTTAAGCT TTGGAAGGGG AGCAAGTCAC 
GGAAACATTC CATCAATAAA TCCCATTAAA GCAACTCCGC CCATGTGGCG CTGCTCTAAG 
GTGATGCAAT TACAGAGAGA AGTACATCCA ACAATATTAA GTTCATTGGA AGGAATTGTA 
GACCAAATGG TTTGGTTTAG AGAAAGCTGG ACAGAGGAAG TTCTTCGACA ACTACGCCAA 
GGCCTAATTA AATGCTATGC CATAGCCTTT GAAAAAAGGG ATACTGTTCA ACATTCTACC 
ATAACACCTC ACACGTTGCA TTTTGTCAAA AAGCTGGGTT CTACGTTTGG CATTGGAATA 
GAAAATGTTC CGGGATCAGT AACCTCCTCA ATTTCTAATT CAGCAGCCTC GGAGTCTCTT 
GCTCGACGCG CCCAAGTTAC TTTTCAAGAT CCAGTATTTC AAAAAATGAA GGAGCAATTC 
ACTAATGACT TCGATTTTTC AAAACCTGGT GCCATGAAAT TGCACAACTT GATATCAAAA 
TTAAAAACAT GGATAAAAGT CCTGGAGACT AAAGTTAAAA AATTACCCAC GTCCTTTTTG 
ATAGAAGACA AGTGTAGGTT TTTATCAAAC TTTAGTCAGA AGACAGCTGA GGTTGAACTT 
CCTGGAGAAT TGTTAATTCC CTTATCATCT CATTATTATG TAAGAATCGC AAGATTCATG 
CCGCGTGTGG AAATTGTACA AAAAAATAAC ACAGCAGCGC GTAGGTTATA TATAAGAGGT 
ACTAACGGGA AAATCTATCC GTACCTTGTA GTTCTTGATT CAGGTTTGGG AGATGCTCGC 
CGAGAGGAAA GAGTTTTGCA GTTAAAACGC ATGTTGAATT ACTATTTAGA AAAACAAAAA 
GAGACAAGTC GAAGATTTCT TAACATAACG GTACCAAGGG TTGTCCCGAT ATCGCCCCAA 
ATGAGATTGG CAGAAGATAA CCCAAACAGT ATTTCATTGT TAAAAATATT TAAAAAATGC 
TGTCAAAGTA TGCAGGTTGA CTACGACATG CCAATAGTTA AGTATTATGA CCGTCTTTCT 
GAAGTACAGG CAAGAGGCAC TCCAACTACA CATACCCTAT TGAGAGAAAT ATTCTCTGAA 
ATTCAATGGA CTATGGTCCC AAAAACATTA CTAAAGCATT GGGCTTTGAA AACATTCTTG 
GCGGCTACTG ACTTTTGGCA TTTCCGAAAA ATGCTTACCC TGCAGTTGGC TTTGGCATTT 
TTATGCGAAC ACGCTTTGAA TCTTACTCGA CTGAATGCGG ATATGATGTA CCTTCATCAA 
GACTCAGGAC TTATGAACAT ATCTTATTTT AAGTTTGATG TAAATGATGA TAAGTGCCAG 
CTTAATCAAC ACCGACCTGT ACCATTTCGC CTGACTCCGA ATGTTGGTGA ATTCATAACA 
CATTTTGGAA TAACTGGACC TTTATCTGCA GCAATTGTGG CAACGGCTCG GTGTTTTATT 
CAACCAAATT ACAAATTAAG CTCAATATTA CAAACCATTT TAAGAGATGA AATAATAGCC 
CTGCAAAAAA AAGGATTCAG AGAATGTAAA CTAATCGAAG GCTCTGAAGA CCGTTATTCC 
GATGGAAATT GTATGGAGCA CTCAGTAAAC ATTGTGAATT CAGCGGTGGA TATCATAATG 
ACGCGTTTTA ATAAAATATC TTATTTTGAT AGCATTGAAA ATAAGAAGAT TTCCGTGCTC 
GTTCAATCGG CAACTAACAT TGATAATCTT TGTCGTATGG ATCCTGCTTG GCATCCCTGG 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303335.1
CDS	58..11283
Translation	MSVIENVPVNTFRNYLNILNDSSSKDELKLKATQELSEHFEMIMQSPAYPSFLDNSLKIFMRILQDGEPQFIQENTMQHIRKLILEMIHRLPITESLRQHVKTIITMMLKILKTDNEENVLVCLRIIIELHKHFRPSFNSEIQLFLGFVKEIYTNLPNHLTSIFETSNDVWVTDLKDLNLEVLLSESYSVRTIHVEKALDSNSQQQIYNLLPRGILSLKVLQELPIIVVLMYQIYKNAVHQEVSEFIPLILTTINLQPTVTRRNSPQKEIYVEFMGAQIKTLSFLAYIVRIFQEVVIASSLSVTSGMLNLMKNCPKEAAHLRKELLIAARHIFATDLRQKFIPSIEQLFDEDLLIGKGVTLDSIRPLAYSTLADLAHHVRQSLNIDVLIKAVNLFSKNVHDESLAVGIQTMSCKLLLNLVDCLRHHSETEPQRSKALLSKLLKVFVKKFETIAKIQLPLIIQKCKGHAFSGALVNSSGNASLSHINAPDLKDDISNIQVSASGSQWIYSVNVAEFRSLVKTLVGGVKTITWGFFNSKFQLTDTKLANHEKIFGPEIVCSYIDLVYYAMEALDIYTINVNPNQQRTSGLISRSKEEKEVLEHFSGIFLMMHSQNFQEIFSTTINFLVERIYKNQSLQVIANSFLANPTTSPLFATVLVEYLLNKMEEMGSNLERSNLYLRLFKLVFGSVSLFPVENEQMLRPHLHKIVNRSMELALISEEPYNYFLLLRALFRSIGGGSHDLLYQEFLPLLPNLLEGLNRLQSGFHKQHMRDLFVELCLTVPVRLSSLLPYLPMLMDPLVSALNGSPTLISQGLRTLELCVDNLQPDFLYDHIQPVRAALMQALWKTLRNQDNAALVAFRVLGKFGGGNRKMMVEPQALSYIINDKPTISIVTYFQEYETPIDFPVDEAIKSAFRALGSNSTDQFYRRQSWEVIRCFLAAFISLDDEKHMLLKLFTHVDFVENKIMNWSTFQHKAGNETVRETHQTALIGMLVASATKDLRDSVCPVMAAVVRHYTMVAIAQQAGPFPQKGYQATHGIDPMILIDALASCMGHEEKELCKPGIACMGIILDTATNIMGNKDRACKLPIIQYLAEKMVSLCYDRPWYSKVGGCQAIQFLCKHMSLRALFQNLFNFLKAFMFVLMDLEGDVSNGAIEITKSYMKSMLEICLTPINECYKNIDLKDLQAKATYEVIHELVRHITSPNTIVREESMVLLKHIGTIQSKTVSEVMDPHKDVLADIIPPKKHLLRHQPANAQIGLMDGNTFCTTLEPRLFTIDLTNTYHKLFFHELLTLSEAEDATLAKLDCYKNVPNLIPLRTSALRALAACHYISDIGYKEKIINIIFKVMESDKSELQTTAFHCMKHFITGVTLEKEKVQSAMRPLLLKLGDHRNLSIPAIKRLSYFTQIFPQMFNEKLSEQILQHCSKIMEIFVSEYKSTSPNVNFFASSKGGEYEQKIVILIEMFFYISASVKYIEKLCQLVLKTEKNLMIEASSPYREALIKFLQRFPTETVDLFLTESLMIDPQWNRLFIYLLKHETGVSFRAVIKSSRYNNLIHYLNTHTEFPEALKYEIQHQAVLIIFTLMESDDQWIPTRQDIVDALKNCWQNYLSTLSSEDVLCDLWHLIGKILLHYFSNNTNDIELLFQLLRALCFRFIPDVYFLRDFLQHTVAQSFTVNWKRNAFFYFVENFNNSFLSEELKAKIITAVIIPCFAVSFDKGEGNKLIGAPPTPYQEDEKNIVSVFINKVFDPDKQYDDAVRIALLQLACLLVERASQHIHDGDANNKRQGNKLRRLMTFAWPCLLSKSSVDPTARYHGHLLLSHIIARLAIHKKIVLQVFHSLLKGHALEARSIVKQALDVLTPAMPLRMEDGNTMLTHWTKKIIVEEGHAMQQLFHILQLIIRHYKVYFPVRHQLVQHLINYMQRLGFPPTASIEHKKLAVDLAEVIIKWELHRIKDDRETKTDGTEEELIQESSVKRSGIDLVETRKKSFDIIRETTVQGKCVMLLKMAMRPEIWPQPFDIKLNWLDKVLATVETPHHNLNNICTGIDFLTFLTTILSPDQLVSIIRPVQRGLSLCIIHQNTRIVRLMHMFLTRIMAIFPPDTQHKHEDLDLLYTAVSKMIAENLTSYEKSPQPNASSLFGTLMILKACTTNNASYIDRILVQFIRVLNHLTRDHINTIGGNTVISQSPDSNALPLELLVLSLELIKNRIFVMSVEIRKLFIGTILVSLIEKSTEVKIIKCIIKMLDEWIKTKEPNVMTQVPSIREKSALLVKLMQNVEKKFTDEIELNIQFLEIINFIYRDEILKQTELTNKLEGAFLNGLRFQNPNVRSKFFEILDSSMRRRLHDRLLYIICSQAWDTIGSHYWIKQCIELLILTANTMMQIQCSNEQFKIPSITSVIPVNSSETQENSFVSFLSSHSESFDIIQTVDDKDDVYDIDLNADRKEDCQQILPNRRVTLVELVYKQAEFLEANRNIRTDQMLVATSQLCHIDTQLAQSVWLSMFPRIWSIFTEDQRCNITKELIPFLSSGTNVNQKDCHPSTLNTFVESLTKCAPPIYIPPNLLAYLGKSHNLWHRAILVLEDMAVNQSMQSKDIDGGENQFSDLDVQQSNNIFDSLSKMYSSMHEEDLWAGLWLKFAHYPETNIAVSYEQMGFFEEAQGAYDLAMTKFKQDLSNGVVNTYVNSELLLWENHWMRCAKELNQWDILLDYAQTNKDKNMFLILESSWRVPDWNLMKIALAKTEQCYLKHYGFKINLYKGYLSILHQEERQTGNIERYVEIASSLCIREWRRLPNIVSHIHLPYLQASQQIMELHEASQIHQGLAQSRNNSLHDMKAIVKTWRNRLPIISDDLSHWSDIFTWRQHHYQIITQHLEQQSDQGSTMLGVHASAQAIISFGKIARKHNLTGVCQETLSRIYTIPSVPIVDCFQKIRQQVKCYLQMPSTSGKNEINEALEVIESTNLKYFTGEMNAEFYALKGLLLAQIGRSEEAGKSFSVAAQLHDGLTKAWAMWGDYMEQIFLKERKITLAVDALICYLQASRNQIESKTRKYIAKVLWFLSYDNNTKILISTLEKHVAGIPPSYWLPWIPQLLCCLEQFEGDVILNLLSQIGRLYPQAVYFPIRTLYLTLKIEQREKHKTAEQAVKSSCSNIDGTTLSFGRGASHGNIPSINPIKATPPMWRCSKVMQLQREVHPTILSSLEGIVDQMVWFRESWTEEVLRQLRQGLIKCYAIAFEKRDTVQHSTITPHTLHFVKKLGSTFGIGIENVPGSVTSSISNSAASESLARRAQVTFQDPVFQKMKEQFTNDFDFSKPGAMKLHNLISKLKTWIKVLETKVKKLPTSFLIEDKCRFLSNFSQKTAEVELPGELLIPLSSHYYVRIARFMPRVEIVQKNNTAARRLYIRGTNGKIYPYLVVLDSGLGDARREERVLQLKRMLNYYLEKQKETSRRFLNITVPRVVPISPQMRLAEDNPNSISLLKIFKKCCQSMQVDYDMPIVKYYDRLSEVQARGTPTTHTLLREIFSEIQWTMVPKTLLKHWALKTFLAATDFWHFRKMLTLQLALAFLCEHALNLTRLNADMMYLHQDSGLMNISYFKFDVNDDKCQLNQHRPVPFRLTPNVGEFITHFGITGPLSAAIVATARCFIQPNYKLSSILQTILRDEIIALQKKGFRECKLIEGSEDRYSDGNCMEHSVNIVNSAVDIIMTRFNKISYFDSIENKKISVLVQSATNIDNLCRMDPAWHPWL

Target ORF information:

RefSeq Version	NM_001316406.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nipped-A, transcript variant F (Nipped-A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316406.1

ORF Insert Sequence:

ATGTCGGTTA TTGAAAATGT ACCGGTGAAT ACATTTCGGA ATTATTTAAA TATTTTAAAT 
GATTCCTCTT CTAAAGACGA ACTTAAGCTG AAAGCTACTC AAGAGCTAAG TGAACACTTT 
GAAATGATAA TGCAAAGTCC GGCATATCCG TCGTTCTTAG ATAATTCTTT AAAAATATTT 
ATGCGGATAC TTCAGGATGG CGAACCTCAG TTCATTCAAG AAAATACAAT GCAACACATA 
CGTAAACTGA TTTTAGAGAT GATTCATCGA TTGCCTATAA CAGAAAGCTT AAGGCAACAT 
GTAAAGACAA TAATAACAAT GATGCTTAAA ATACTTAAAA CTGACAACGA AGAAAATGTT 
CTTGTATGTC TTCGCATTAT AATTGAACTG CATAAGCATT TTCGTCCATC ATTTAATTCT 
GAAATTCAAC TATTTTTGGG ATTTGTTAAG GAAATCTATA CAAATCTTCC TAACCATCTT 
ACATCTATAT TTGAAACATC GAATGATGTT TGGGTTACCG ATTTAAAGGA CTTAAATTTG 
GAAGTACTTT TATCCGAATC ATATTCTGTT AGAACTATCC ATGTCGAAAA GGCACTTGAT 
TCAAATTCTC AACAACAAAT TTATAATCTC CTTCCTCGTG GAATATTATC TTTGAAAGTT 
TTACAAGAGC TCCCAATAAT AGTAGTACTA ATGTACCAAA TATATAAAAA CGCAGTACAT 
CAAGAAGTTT CTGAATTCAT TCCTCTTATA TTGACTACTA TTAATTTGCA ACCAACAGTA 
ACACGAAGAA ATTCTCCTCA GAAAGAAATA TATGTCGAAT TCATGGGTGC ACAAATTAAA 
ACTCTGTCAT TTTTGGCGTA CATTGTCCGA ATATTTCAGG AAGTGGTAAT AGCTAGTTCA 
TTATCTGTAA CCAGCGGAAT GCTCAACCTT ATGAAAAATT GTCCCAAGGA AGCCGCTCAT 
CTCAGAAAAG AACTTTTAAT TGCTGCCCGT CATATATTTG CTACAGATTT ACGTCAAAAA 
TTTATTCCAT CAATCGAACA ATTGTTTGAT GAGGATTTGC TTATTGGAAA AGGAGTTACA 
TTGGATTCTA TTCGGCCATT GGCGTATTCA ACTTTAGCTG ACCTAGCTCA CCACGTTCGC 
CAAAGCCTAA ATATAGACGT TTTAATAAAG GCAGTGAATC TCTTTTCAAA AAATGTTCAT 
GATGAATCAC TTGCTGTGGG AATACAAACT ATGTCATGCA AGCTTTTGTT AAATCTAGTT 
GATTGTTTAC GTCATCACAG TGAAACAGAA CCACAAAGAT CAAAAGCTCT ACTCTCAAAA 
CTGTTAAAAG TGTTTGTAAA AAAATTTGAA ACTATAGCTA AAATTCAGCT TCCATTAATA 
ATTCAAAAGT GCAAAGGGCA CGCATTTTCT GGAGCATTGG TAAATTCATC TGGCAACGCT 
TCCTTATCAC ACATTAACGC GCCAGATCTA AAAGACGACA TATCCAACAT CCAAGTATCT 
GCATCTGGAT CACAATGGAT ATATTCCGTC AATGTCGCTG AATTTCGAAG CTTAGTAAAA 
ACCCTTGTTG GTGGAGTAAA AACAATAACT TGGGGTTTTT TTAACTCAAA GTTTCAGCTA 
ACTGATACCA AACTGGCCAA CCATGAAAAA ATTTTTGGAC CTGAAATCGT ATGTTCTTAC 
ATCGACTTGG TTTACTATGC GATGGAGGCT CTAGATATAT ACACTATAAA CGTTAATCCA 
AATCAACAGA GAACGTCTGG GTTGATTTCC AGAAGTAAAG AAGAAAAAGA AGTCTTGGAG 
CATTTTAGTG GCATATTTTT AATGATGCAC TCCCAAAACT TTCAAGAAAT ATTTTCTACT 
ACGATTAATT TTTTGGTGGA AAGGATCTAC AAAAATCAGT CCTTACAAGT GATAGCGAAC 
TCCTTTTTAG CAAATCCTAC TACTTCACCT CTGTTTGCCA CTGTTCTAGT GGAATATCTT 
TTGAACAAAA TGGAAGAAAT GGGATCAAAC TTGGAACGCT CCAACTTGTA TCTACGATTA 
TTCAAGTTGG TATTCGGCAG CGTAAGTCTT TTTCCAGTCG AAAATGAACA GATGTTGCGT 
CCACATCTTC ATAAAATAGT CAATAGATCC ATGGAATTGG CTTTGATATC GGAGGAGCCG 
TATAACTATT TTTTACTTCT GAGGGCATTG TTTAGATCGA TTGGAGGAGG AAGCCATGAC 
CTATTATACC AAGAGTTTCT GCCACTTTTA CCAAACTTAT TAGAAGGATT AAATCGCCTA 
CAGAGTGGAT TTCATAAGCA GCACATGCGA GATCTTTTCG TGGAACTTTG CCTTACAGTT 
CCGGTCCGCT TGTCATCTTT ACTGCCCTAT TTACCAATGT TGATGGACCC ATTAGTTTCT 
GCGTTAAACG GATCTCCAAC ATTAATAAGT CAGGGCCTGC GAACCTTAGA GCTTTGCGTT 
GACAATTTGC AGCCAGACTT TTTATATGAT CATATACAAC CAGTAAGAGC TGCTTTAATG 
CAAGCATTGT GGAAAACTTT AAGAAATCAG GATAACGCCG CTTTGGTTGC CTTTCGCGTT 
CTAGGAAAAT TTGGTGGCGG CAATCGAAAA ATGATGGTGG AGCCACAAGC TTTGTCGTAT 
ATTATAAACG ACAAGCCCAC CATTTCCATT GTAACTTATT TTCAGGAATA CGAAACACCA 
ATAGATTTTC CTGTTGATGA AGCGATTAAG TCCGCCTTTA GAGCATTGGG ATCAAATTCT 
ACTGACCAGT TTTATAGGAG GCAAAGCTGG GAAGTAATCA GGTGCTTCTT AGCTGCTTTT 
ATTAGTTTGG ATGATGAAAA ACATATGCTG CTGAAACTTT TTACACATGT AGATTTTGTT 
GAAAATAAAA TTATGAATTG GTCTACGTTT CAACATAAAG CCGGGAACGA AACTGTACGA 
GAAACTCATC AGACAGCCCT CATAGGTATG TTGGTTGCAT CAGCAACAAA GGACTTGCGT 
GATTCAGTGT GCCCAGTAAT GGCCGCTGTT GTGAGGCATT ATACTATGGT AGCTATTGCA 
CAGCAGGCCG GACCTTTTCC ACAAAAAGGC TACCAAGCCA CTCATGGAAT TGACCCCATG 
ATATTAATAG ATGCTTTAGC CTCTTGCATG GGTCATGAAG AAAAGGAGTT ATGCAAACCC 
GGAATAGCTT GTATGGGTAT TATACTTGAC ACTGCAACAA ATATTATGGG TAATAAAGAC 
AGAGCGTGTA AATTGCCCAT AATACAGTAT CTAGCAGAAA AAATGGTATC CTTATGCTAT 
GATCGGCCAT GGTACTCAAA AGTAGGCGGT TGCCAAGCAA TTCAATTTTT ATGCAAACAT 
ATGTCTTTGC GAGCCCTTTT TCAAAATCTC TTTAATTTTT TAAAAGCTTT CATGTTTGTA 
TTAATGGACC TAGAGGGAGA CGTTTCTAAT GGGGCTATTG AAATAACAAA AAGTTATATG 
AAAAGCATGT TGGAAATTTG TTTAACGCCA ATAAATGAAT GTTATAAGAA TATAGATCTA 
AAGGACTTAC AAGCCAAAGC CACTTATGAA GTTATTCACG AATTAGTGCG GCACATCACA 
AGCCCTAATA CCATTGTTAG AGAAGAATCT ATGGTACTAC TTAAACATAT TGGTACAATA 
CAATCCAAAA CCGTTTCTGA AGTTATGGAT CCTCATAAAG ACGTTCTCGC TGACATTATA 
CCGCCAAAGA AGCATTTATT GAGACATCAA CCCGCAAATG CGCAAATCGG ACTTATGGAT 
GGCAATACAT TCTGCACAAC TTTGGAGCCA AGACTTTTTA CAATTGATTT GACGAATACC 
TACCACAAAT TATTTTTTCA CGAGTTACTT ACACTAAGTG AAGCCGAAGA TGCCACTTTA 
GCAAAGTTGG ACTGTTACAA AAACGTTCCG AATCTTATAC CACTTAGGAC TAGTGCGTTG 
CGAGCTTTAG CAGCATGCCA CTATATAAGT GATATCGGAT ACAAAGAAAA AATAATAAAT 
ATAATTTTTA AAGTAATGGA AAGTGATAAA TCAGAACTTC AGACAACTGC GTTTCATTGC 
ATGAAGCATT TTATAACTGG AGTTACTCTG GAAAAAGAAA AGGTACAATC TGCAATGCGC 
CCACTGCTAT TAAAATTGGG CGACCACAGA AATTTATCGA TACCAGCAAT AAAACGCTTA 
TCTTACTTTA CTCAGATCTT TCCTCAAATG TTCAATGAGA AGTTAAGTGA GCAAATTTTG 
CAACATTGCT CTAAAATTAT GGAAATATTT GTTAGTGAAT ACAAATCCAC AAGTCCAAAC 
GTTAATTTTT TTGCATCATC GAAAGGTGGA GAATATGAAC AGAAAATTGT CATACTAATC 
GAAATGTTTT TCTATATATC AGCATCAGTA AAATACATTG AAAAGCTGTG CCAACTTGTT 
TTAAAAACGG AAAAAAACTT AATGATTGAG GCATCGAGTC CATATCGAGA AGCCCTAATT 
AAGTTTCTTC AACGATTTCC AACAGAAACT GTGGATTTAT TTTTAACGGA ATCTCTTATG 
ATAGATCCGC AATGGAATCG TCTTTTTATA TACTTGTTGA AACACGAAAC GGGAGTATCA 
TTTCGAGCAG TTATTAAAAG TAGTCGGTAT AATAATTTAA TACATTATTT AAATACACAC 
ACAGAATTTC CAGAAGCACT CAAATATGAA ATTCAGCACC AAGCCGTTTT AATAATTTTT 
ACATTAATGG AATCAGACGA TCAATGGATA CCAACACGAC AAGATATTGT TGACGCCCTT 
AAAAATTGTT GGCAGAATTA TTTAAGTACA TTAAGCTCTG AAGACGTTCT GTGTGACTTA 
TGGCATTTGA TTGGAAAAAT ACTTTTGCAT TATTTTTCTA ACAATACAAA TGACATTGAA 
CTGCTGTTTC AATTATTACG TGCTTTATGC TTCCGATTTA TACCGGATGT CTATTTCCTG 
CGGGACTTCT TGCAACACAC AGTAGCACAA AGTTTTACGG TTAACTGGAA ACGAAACGCA 
TTCTTTTACT TTGTTGAAAA CTTTAATAAC AGCTTTCTTT CAGAAGAGCT AAAGGCAAAA 
ATAATAACAG CAGTCATAAT ACCCTGTTTT GCTGTAAGTT TCGATAAAGG TGAAGGAAAT 
AAACTAATTG GGGCTCCTCC GACCCCGTAT CAAGAAGATG AAAAAAATAT TGTGTCCGTT 
TTTATTAATA AAGTGTTTGA CCCAGATAAA CAGTATGATG ATGCAGTAAG AATTGCTCTA 
TTACAACTAG CATGCTTATT GGTAGAACGT GCATCTCAGC ACATACATGA CGGAGATGCT 
AATAATAAGC GCCAAGGCAA TAAGCTTAGA CGACTCATGA CCTTTGCCTG GCCCTGTTTG 
CTGAGTAAAA GTTCAGTGGA TCCTACTGCA CGATACCACG GTCACTTATT ACTTTCGCAT 
ATAATCGCAA GATTGGCCAT TCACAAGAAA ATAGTTTTAC AAGTATTTCA TTCCTTGTTG 
AAAGGACACG CATTAGAGGC CAGATCTATT GTTAAACAGG CACTTGATGT TCTAACGCCA 
GCTATGCCGT TGCGAATGGA GGATGGAAAT ACTATGCTAA CACACTGGAC AAAAAAAATT 
ATAGTTGAAG AAGGTCACGC TATGCAGCAG TTATTTCATA TTTTGCAGCT CATTATTCGT 
CATTATAAAG TGTATTTTCC GGTTCGACAT CAACTAGTGC AACACTTGAT TAACTATATG 
CAGCGCCTTG GGTTTCCTCC TACTGCATCT ATTGAACATA AAAAACTTGC TGTGGACTTA 
GCAGAAGTTA TTATAAAATG GGAACTTCAT CGTATTAAGG ATGATCGTGA AACTAAAACA 
GATGGAACCG AAGAGGAGTT AATACAGGAA AGCTCAGTAA AACGATCAGG GATCGATTTA 
GTAGAAACTC GAAAAAAGTC ATTCGATATT ATAAGAGAAA CAACAGTCCA AGGAAAGTGC 
GTTATGCTAT TAAAAATGGC GATGCGTCCC GAGATCTGGC CTCAACCATT TGATATTAAG 
CTTAATTGGC TTGATAAAGT TCTTGCTACA GTGGAAACCC CTCATCACAA CTTAAACAAC 
ATATGTACTG GAATAGATTT TTTAACATTT TTAACTACTA TACTAAGTCC AGATCAGTTG 
GTGTCAATTA TACGACCGGT TCAACGAGGC CTGTCCTTGT GTATAATTCA TCAAAATACA 
CGAATTGTGC GGTTAATGCA TATGTTTTTG ACGCGAATAA TGGCAATTTT TCCACCTGAC 
ACCCAACACA AGCATGAAGA TCTTGATTTG TTATATACCG CTGTTAGTAA AATGATCGCT 
GAAAACCTAA CAAGTTACGA AAAGAGTCCA CAACCGAATG CTTCTTCGCT TTTTGGTACC 
TTAATGATTT TGAAAGCGTG CACTACTAAT AATGCAAGTT ATATTGATCG AATTTTAGTC 
CAGTTTATAA GGGTTCTGAA TCATCTTACA AGGGATCATA TTAACACCAT TGGTGGTAAC 
ACTGTTATTA GCCAATCTCC TGATTCAAAT GCTTTACCTT TAGAACTCTT AGTTCTCTCT 
TTGGAATTAA TCAAGAATAG GATCTTTGTA ATGAGTGTGG AAATCAGAAA ACTTTTCATA 
GGCACCATTT TGGTTAGTCT TATAGAAAAG AGCACGGAGG TAAAAATTAT AAAATGTATA 
ATTAAAATGC TGGATGAATG GATTAAAACG AAAGAGCCAA ATGTTATGAC ACAAGTTCCT 
TCTATTCGCG AAAAGTCGGC CTTGCTGGTA AAGTTAATGC AAAACGTTGA AAAGAAATTT 
ACTGATGAAA TAGAGCTTAA TATACAATTC TTGGAAATCA TAAATTTTAT ATATAGAGAT 
GAAATTCTTA AACAAACTGA GTTAACAAAC AAACTAGAGG GAGCTTTTTT AAACGGATTA 
CGTTTTCAAA ATCCAAACGT ACGCTCGAAA TTTTTTGAGA TTTTAGACTC ATCAATGCGG 
CGTAGACTTC ACGATCGATT GTTATATATA ATTTGTTCCC AAGCTTGGGA CACAATTGGT 
TCCCATTATT GGATAAAACA ATGCATTGAA TTGCTTATTT TAACAGCCAA TACAATGATG 
CAAATTCAAT GTTCAAATGA ACAATTTAAA ATACCCAGCA TTACTTCAGT CATTCCAGTG 
AATTCATCAG AAACACAGGA AAATTCCTTT GTATCCTTCT TATCCTCTCA TTCCGAATCT 
TTTGACATTA TACAAACTGT TGATGATAAA GACGACGTGT ATGATATCGA TTTAAATGCT 
GATCGCAAAG AAGATTGTCA ACAAATACTA CCAAATCGAC GTGTTACTCT TGTTGAACTA 
GTTTACAAGC AAGCTGAATT TTTAGAAGCA AACCGAAATA TTAGGACCGA CCAGATGCTT 
GTCGCCACAT CTCAGCTATG TCATATTGAT ACACAGTTAG CTCAAAGCGT ATGGTTATCT 
ATGTTTCCAC GTATTTGGAG TATATTCACT GAAGATCAAA GGTGTAATAT CACAAAAGAA 
CTGATTCCCT TTTTATCGTC TGGAACTAAC GTTAATCAAA AAGACTGCCA TCCAAGTACA 
TTAAATACTT TTGTAGAGAG TTTAACTAAA TGTGCGCCAC CCATATATAT TCCACCTAAT 
TTATTAGCAT ACCTAGGCAA ATCTCATAAC CTATGGCATA GAGCTATACT TGTTTTAGAA 
GATATGGCCG TTAATCAATC AATGCAATCC AAGGATATTG ATGGCGGTGA AAATCAATTC 
TCTGACTTGG ATGTACAACA ATCAAATAAT ATATTTGATT CACTTTCAAA AATGTATTCT 
TCGATGCATG AGGAAGATCT TTGGGCTGGC CTATGGCTTA AATTTGCACA CTACCCGGAA 
ACAAATATAG CTGTTTCGTA TGAGCAAATG GGATTTTTCG AAGAAGCCCA AGGTGCCTAT 
GATCTAGCAA TGACCAAATT TAAACAAGAT CTAAGTAATG GTGTAGTTAA TACATATGTT 
AATAGTGAAT TATTATTGTG GGAAAATCAC TGGATGCGAT GTGCTAAAGA ATTGAACCAA 
TGGGACATTT TACTGGACTA TGCCCAAACT AATAAGGACA AAAATATGTT TTTGATTCTG 
GAAAGTTCGT GGCGCGTACC TGATTGGAAT TTGATGAAAA TCGCACTGGC TAAAACAGAA 
CAATGCTATT TAAAACACTA CGGCTTTAAA ATCAACCTTT ACAAAGGGTA TTTGAGTATT 
CTCCACCAAG AAGAAAGGCA AACAGGCAAT ATCGAACGAT ATGTTGAAAT TGCATCCAGC 
TTATGCATTC GTGAATGGCG TCGATTGCCG AACATAGTTT CACATATTCA TTTGCCATAT 
CTTCAAGCAT CACAACAAAT TATGGAGCTT CATGAAGCAA GTCAAATCCA TCAGGGACTT 
GCTCAATCGC GCAACAATTC ACTTCACGAT ATGAAAGCTA TCGTGAAAAC TTGGCGTAAT 
CGTTTACCTA TTATTTCTGA TGACTTATCG CATTGGAGTG ACATATTTAC ATGGAGACAA 
CATCACTACC AAATAATAAC ACAACACCTA GAACAACAAT CGGATCAAGG AAGTACAATG 
CTAGGAGTTC ACGCATCAGC ACAAGCTATA ATTTCTTTTG GAAAAATAGC TCGGAAACAC 
AATTTGACTG GTGTTTGTCA GGAGACGTTG TCCAGGATAT ATACAATTCC GTCTGTTCCT 
ATCGTGGATT GTTTTCAGAA AATTCGGCAG CAAGTAAAAT GCTACCTGCA AATGCCCTCA 
ACATCTGGAA AAAATGAAAT TAATGAAGCC TTGGAAGTAA TTGAGTCCAC GAATTTAAAA 
TACTTCACTG GTGAAATGAA TGCTGAATTT TACGCTCTAA AGGGGCTATT ATTAGCACAA 
ATTGGAAGAT CAGAAGAGGC TGGAAAATCA TTTAGTGTTG CTGCTCAGCT TCATGATGGT 
CTTACCAAAG CCTGGGCAAT GTGGGGTGAC TATATGGAAC AAATATTTTT AAAAGAAAGG 
AAAATCACAT TAGCCGTCGA TGCTTTAATT TGTTATTTAC AAGCAAGCAG AAATCAAATT 
GAAAGCAAAA CCCGAAAATA TATTGCAAAA GTTTTGTGGT TTCTGTCTTA TGATAATAAT 
ACTAAAATCC TCATAAGCAC TTTAGAAAAG CATGTGGCAG GCATTCCACC CTCTTATTGG 
CTACCATGGA TTCCTCAGTT GCTCTGTTGC TTAGAACAGT TCGAAGGGGA TGTTATATTA 
AATCTCTTAA GCCAAATTGG ACGCCTTTAT CCTCAAGCGG TATATTTCCC GATTCGGACT 
TTATATTTGA CTTTAAAAAT CGAACAACGC GAAAAACATA AAACTGCTGA ACAGGCTGTA 
AAAAGTTCAT GCTCGAACAT CGATGGAACT ACTTTAAGCT TTGGAAGGGG AGCAAGTCAC 
GGAAACATTC CATCAATAAA TCCCATTAAA GCAACTCCGC CCATGTGGCG CTGCTCTAAG 
GTGATGCAAT TACAGAGAGA AGTACATCCA ACAATATTAA GTTCATTGGA AGGAATTGTA 
GACCAAATGG TTTGGTTTAG AGAAAGCTGG ACAGAGGAAG TTCTTCGACA ACTACGCCAA 
GGCCTAATTA AATGCTATGC CATAGCCTTT GAAAAAAGGG ATACTGTTCA ACATTCTACC 
ATAACACCTC ACACGTTGCA TTTTGTCAAA AAGCTGGGTT CTACGTTTGG CATTGGAATA 
GAAAATGTTC CGGGATCAGT AACCTCCTCA ATTTCTAATT CAGCAGCCTC GGAGTCTCTT 
GCTCGACGCG CCCAAGTTAC TTTTCAAGAT CCAGTATTTC AAAAAATGAA GGAGCAATTC 
ACTAATGACT TCGATTTTTC AAAACCTGGT GCCATGAAAT TGCACAACTT GATATCAAAA 
TTAAAAACAT GGATAAAAGT CCTGGAGACT AAAGTTAAAA AATTACCCAC GTCCTTTTTG 
ATAGAAGACA AGTGTAGGTT TTTATCAAAC TTTAGTCAGA AGACAGCTGA GGTTGAACTT 
CCTGGAGAAT TGTTAATTCC CTTATCATCT CATTATTATG TAAGAATCGC AAGATTCATG 
CCGCGTGTGG AAATTGTACA AAAAAATAAC ACAGCAGCGC GTAGGTTATA TATAAGAGGT 
ACTAACGGGA AAATCTATCC GTACCTTGTA GTTCTTGATT CAGGTTTGGG AGATGCTCGC 
CGAGAGGAAA GAGTTTTGCA GTTAAAACGC ATGTTGAATT ACTATTTAGA AAAACAAAAA 
GAGACAAGTC GAAGATTTCT TAACATAACG GTACCAAGGG TTGTCCCGAT ATCGCCCCAA 
ATGAGATTGG CAGAAGATAA CCCAAACAGT ATTTCATTGT TAAAAATATT TAAAAAATGC 
TGTCAAAGTA TGCAGGTTGA CTACGACATG CCAATAGTTA AGTATTATGA CCGTCTTTCT 
GAAGTACAGG CAAGAGGCAC TCCAACTACA CATACCCTAT TGAGAGAAAT ATTCTCTGAA 
ATTCAATGGA CTATGGTCCC AAAAACATTA CTAAAGCATT GGGCTTTGAA AACATTCTTG 
GCGGCTACTG ACTTTTGGCA TTTCCGAAAA ATGCTTACCC TGCAGTTGGC TTTGGCATTT 
TTATGCGAAC ACGCTTTGAA TCTTACTCGA CTGAATGCGG ATATGATGTA CCTTCATCAA 
GACTCAGGAC TTATGAACAT ATCTTATTTT AAGTTTGATG TAAATGATGA TAAGTGCCAG 
CTTAATCAAC ACCGACCTGT ACCATTTCGC CTGACTCCGA ATGTTGGTGA ATTCATAACA 
CATTTTGGAA TAACTGGACC TTTATCTGCA GCAATTGTGG CAACGGCTCG GTGTTTTATT 
CAACCAAATT ACAAATTAAG CTCAATATTA CAAACCATTT TAAGAGATGA AATAATAGCC 
CTGCAAAAAA AAGGATTCAG AGAATGTAAA CTAATCGAAG GCTCTGAAGA CCGTTATTCC 
GATGGAAATT GTATGGAGCA CTCAGTAAAC ATTGTGAATT CAGCGGTGGA TATCATAATG 
ACGCGTTTTA ATAAAATATC TTATTTTGAT AGCATTGAAA ATAAGAAGAT TTCCGTGCTC 
GTTCAATCGG CAACTAACAT TGATAATCTT TGTCGTATGG ATCCTGCTTG GCATCCCTGG 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nipped-A cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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