step cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	step
Entrez Gene ID	35425
Full Name	steppke
Synonyms	CG11628,CG11633,CYH1,Dmel\CG11628,GPH,GRP1,GRP1/cytohesin 1,Grp1,Step,cytohesin/GRP1,l(2)SH0323,l(2)SH2 0323,l(2)k08110,stepk
General protein information	Preferred Names steppke Names CG11628 gene product from transcript CG11628-RE CG11628-PA CG11628-PC CG11628-PD CG11628-PE general receptor for phosphoinositides 1 general receptor for phosphoinositides-1 lethal (2) SH0323 lethal (2) k08110 step-PA step-PC step-PD step-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 40A1-40A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273749.1	NP_001260678.1	steppke, isoform E
NM_001169570.2	NP_001163041.1	steppke, isoform D
NM_001042910.3	NP_001036375.2	steppke, isoform C
NM_136276.4	NP_610120.1	steppke, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cyth1	NP_001106170.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008737	XP_314852.3
Xenopus tropicalis (tropical clawed frog)	cyth2	NP_001005039.1
Homo sapiens (human)	CYTH1	NP_004753.1
Gallus gallus (chicken)	CYTH1	NP_001026276.1
Danio rerio (zebrafish)	cyth1a	NP_999847.1
Canis lupus familiaris (dog)	CYTH1	XP_848819.1
Drosophila melanogaster (fruit fly)	step	NP_610120.1
	CYTH1	XP_001108434.1
Bos taurus (cattle)	CYTH1	XP_002696202.3
Rattus norvegicus (Norway rat)	Cyth1	NP_446362.1
Pan troglodytes (chimpanzee)	CYTH1	XP_511712.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It is proposed that a global distribution of Step normally sets an inhibitory threshold for Rho1 activity to restrict early cell formation to the posterior.
Title: Germ Cell Segregation from the Drosophila Soma Is Controlled by an Inhibitory Threshold Set by the Arf-GEF Steppke.

Steppke controls initial plasma membrane furrow ingression during the syncytial nuclear divisions and cellularization of the Drosophila embryo. Acting at the tips of ingressing furrows, Steppke promotes local endocytic events through its Arf-GEF activity.
Title: An Arf-GEF regulates antagonism between endocytosis and the cytoskeleton for Drosophila blastoderm development.

Step may be part of a larger signaling scaffold coordinating receptor tyrosine kinase-dependent MAPK activation
Title: The Drosophila Arf GEF Steppke controls MAPK activation in EGFR signaling.

identification of a new component of insulin signalling in Drosophila, the steppke gene (step)
Title: The cytohesin Steppke is essential for insulin signalling in Drosophila.

The following step gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the step cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15148	NM_136276.4 Latest version!	Drosophila melanogaster steppke (step), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa15147	NM_001169570.2 Latest version!	Drosophila melanogaster steppke (step), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15147	NM_001273749.1 Latest version!	Drosophila melanogaster steppke (step), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15147	NM_001042910.3 Latest version!	Drosophila melanogaster steppke (step), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

step ( NM_136276.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610120.1 Drosophila melanogaster steppke (step), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15148
Clone ID Related Accession (Same CDS sequence)	NM_136276.4
Accession Version	NM_136276.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	steppke, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_136276.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTAGCG CAATGGACAA TTTCGACAAC AGATCGTACG GTGCCATATC AAAGGGGCGC 
AGTGAATTCT CAATGCCAGA ACTTACACCG GAGCAGCAAA AACTGCTGAT TGAGCTGCGA 
CGCAAGAAGC AGGAACTTTT ACTCGAAATA CAGCAAATTA AAGATGAGTT GTGCGAGGTT 
GTATCGGAAA TGGAGGCGCT GGATGTGCCC GAAGACTGCA AGCATTCCAA TAAAGATAAA 
CAGATGTCCA TTGGCCGTAA AAAGTTTAAC ATGGACCCAA AAAAAGGCAT TGAGTATCTC 
GTTGAAAACA GGCTGCTACG TCATGATCCC CAGGACGTTG CCCACTTTCT CTATAAGGGA 
GAGGGACTAA ATAAAACAGC CATTGGCGAT TACCTCGGCG AGAAAAACGA TTTTAATGAG 
GATGTGCTTA AGGCTTTTGT GGCGCTTCAC GATTTCACCA ATCTCATTCT CGTACAAGCC 
CTCAGACAAT TTCTGTGGTC GTTTCGCCTG CCCGGCGAGG CACAAAAGAT CGATCGCATG 
ATGGAGACCT TCGCACAGCG CTACTGTCAA CTAAATCCAG ATATATTCAC CAACACGGAC 
ACATGCTATG TGCTCAGCTT CGCAATTATA ATGCTAAATA CCTCACTACA CAATCCATCT 
GTTAAAGACA AACCCACCGT TGATCAATTC ATATCGATGA ATCGTGGCAT CAACAACGGC 
GGAGATTTGC CCCGTGGCCT GCTGGAATCC CTTTACGAGT CAATTCGAAC AGAGCCATTT 
AAAATACCAC AGGATGATGG AAATGATTTG ATGCACACCT TCTTCAATCC CGACAAGGAG 
GGCTGGCTGT GGAAGCAAGG CGGCAGATAC AAATCGTGGA AACGACGTTG GTTCATTTTG 
AACGATAATT GCTTATACTA TTTTGAATAC ACAACGGATA AGGAACCACG CGGAATTATA 
CCGCTGGAGA ATATATCGGT GCGCGAGATT CACGATCGCA GCAAACCGCA TTGCTTTGAG 
TTGTTTGCGA CGGGCGGTGC TGATATAATC AAGGCATGCA AGACTGACTC TGAGGGCAAA 
GTGGTAGAGG GCAAGCATAC GGTGTACCGC ATGTCCGCTG CCACGGAGGA AGATCAGCAG 
GAGTGGATCA AGCGTTTGAC GCAGTCCATA AGCCATAATC CGTTTTATGA CATCCTGGTA 
CAAAGAAAGA AAAAGGCACT CAGCAAGAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610120.1
CDS	225..1457
Translation	MISAMDNFDNRSYGAISKGRSEFSMPELTPEQQKLLIELRRKKQELLLEIQQIKDELCEVVSEMEALDVPEDCKHSNKDKQMSIGRKKFNMDPKKGIEYLVENRLLRHDPQDVAHFLYKGEGLNKTAIGDYLGEKNDFNEDVLKAFVALHDFTNLILVQALRQFLWSFRLPGEAQKIDRMMETFAQRYCQLNPDIFTNTDTCYVLSFAIIMLNTSLHNPSVKDKPTVDQFISMNRGINNGGDLPRGLLESLYESIRTEPFKIPQDDGNDLMHTFFNPDKEGWLWKQGGRYKSWKRRWFILNDNCLYYFEYTTDKEPRGIIPLENISVREIHDRSKPHCFELFATGGADIIKACKTDSEGKVVEGKHTVYRMSAATEEDQQEWIKRLTQSISHNPFYDILVQRKKKALSKS

Target ORF information:

RefSeq Version	NM_136276.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster steppke (step), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136276.4

ORF Insert Sequence:

ATGATTAGCG CAATGGACAA TTTCGACAAC AGATCGTACG GTGCCATATC AAAGGGGCGC 
AGTGAATTCT CAATGCCAGA ACTTACACCG GAGCAGCAAA AACTGCTGAT TGAGCTGCGA 
CGCAAGAAGC AGGAACTTTT ACTCGAAATA CAGCAAATTA AAGATGAGTT GTGCGAGGTT 
GTATCGGAAA TGGAGGCGCT GGATGTGCCC GAAGACTGCA AGCATTCCAA TAAAGATAAA 
CAGATGTCCA TTGGCCGTAA AAAGTTTAAC ATGGACCCAA AAAAAGGCAT TGAGTATCTC 
GTTGAAAACA GGCTGCTACG TCATGATCCC CAGGACGTTG CCCACTTTCT CTATAAGGGA 
GAGGGACTAA ATAAAACAGC CATTGGCGAT TACCTCGGCG AGAAAAACGA TTTTAATGAG 
GATGTGCTTA AGGCTTTTGT GGCGCTTCAC GATTTCACCA ATCTCATTCT CGTACAAGCC 
CTCAGACAAT TTCTGTGGTC GTTTCGCCTG CCCGGCGAGG CACAAAAGAT CGATCGCATG 
ATGGAGACCT TCGCACAGCG CTACTGTCAA CTAAATCCAG ATATATTCAC CAACACGGAC 
ACATGCTATG TGCTCAGCTT CGCAATTATA ATGCTAAATA CCTCACTACA CAATCCATCT 
GTTAAAGACA AACCCACCGT TGATCAATTC ATATCGATGA ATCGTGGCAT CAACAACGGC 
GGAGATTTGC CCCGTGGCCT GCTGGAATCC CTTTACGAGT CAATTCGAAC AGAGCCATTT 
AAAATACCAC AGGATGATGG AAATGATTTG ATGCACACCT TCTTCAATCC CGACAAGGAG 
GGCTGGCTGT GGAAGCAAGG CGGCAGATAC AAATCGTGGA AACGACGTTG GTTCATTTTG 
AACGATAATT GCTTATACTA TTTTGAATAC ACAACGGATA AGGAACCACG CGGAATTATA 
CCGCTGGAGA ATATATCGGT GCGCGAGATT CACGATCGCA GCAAACCGCA TTGCTTTGAG 
TTGTTTGCGA CGGGCGGTGC TGATATAATC AAGGCATGCA AGACTGACTC TGAGGGCAAA 
GTGGTAGAGG GCAAGCATAC GGTGTACCGC ATGTCCGCTG CCACGGAGGA AGATCAGCAG 
GAGTGGATCA AGCGTTTGAC GCAGTCCATA AGCCATAATC CGTTTTATGA CATCCTGGTA 
CAAAGAAAGA AAAAGGCACT CAGCAAGAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

step ( NM_001273749.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260678.1 Drosophila melanogaster steppke (step), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa15147
Clone ID Related Accession (Same CDS sequence)	NM_001273749.1 , NM_001169570.2 , NM_001042910.3
Accession Version	NM_001273749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	steppke, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCCC TCCATCAGAA GCGTCCCAGC ATAAGTAACT GGTTCTCCTC GCTTCGTCGG 
CAGCCCAAAA ACAAGAAGTG CTCCCCCGGC TTGGGAGGAA GTGAAAGTGT TGGCAGAAAG 
CAGCCATTGC AGAGGAGCTG CCTTGACCTA TCCACGACGC GAGTTGGTTG CGATGGTTGT 
GTAAGTTTGG AGTTCTTTCA ATCGGAATCC CCTGAAAGTC GGCGAAGCAA CAGCACTTTC 
TATGTCAACA CCCAAAATGT ATCGTCTGCT GTTTTTAAAG ACAATCGTCA GCCTCTATTG 
GAAAGGGGCA GCGGTAGTGA TAGTAGCCCT AGCAGCGTTT GCGTTCGATG CTCATGCAAT 
TCGATAACGA AAAGCGGGGA CAGAAATGAA ACACCTACAA TGGCCACGCC TAAGTCGGAC 
ATTAAGATCG CGCTGAAACC TCCACCCAAG CCCCGCTCCC CTTCCATGGC TCCCTACACT 
AGTGTGGCTA CATATAACAT CACGAAATTA GCGCCGGACA GCCCACCCCC CGCCAGCCCC 
ACATTAAGCA CTCAAACCGT AAATTGCAGC GATGTAACCA CGAAGGTCAC AGAGGTACAG 
CGCCTACCCT GCGATGAACC CAACACTGAA CTCATAAGCA AGCGAACGGA GTACAAGCAC 
ACAACCATCA CAACAACGAC AAGAACATTA ATTGTGTCGC GACCCGTGGA ACTGCTTTTA 
AATGAAAATG GCGAAATAAT CGCAAGGCGA TCCCCCCGCA AATCGCGCTC TAATTCCCTG 
GGCCGCATGC TTGACGTGCA GAATGAAATT GGAACTGGCG GGTTGTTTAT GGAAGCGGAT 
CCTTGTTCGA AAAACAGTTT ACCGCCTCCA CCCTATATCG ACGATATGCG CTTCATTGAT 
GACAGCTCCA ACTCACAAAG TGAGTCCGAG CGGAACTCTA ACATACACAA AACCAACAAA 
TATACTTCAA AGAATAATAA CAACGACAGC AAGCGGGAGA GCCAGCAATC GAATCTGTGC 
GAGAGTTGCC GCATGCTTCT CGATCGAAAT TACATAAATA CTGAACAGAG TGACAATCTA 
GTCATCCTCA GGCGACCATC AAAGCAAATT AAAGATGAGT TGTGCGAGGT TGTATCGGAA 
ATGGAGGCGC TGGATGTGCC CGAAGACTGC AAGCATTCCA ATAAAGATAA ACAGATGTCC 
ATTGGCCGTA AAAAGTTTAA CATGGACCCA AAAAAAGGCA TTGAGTATCT CGTTGAAAAC 
AGGCTGCTAC GTCATGATCC CCAGGACGTT GCCCACTTTC TCTATAAGGG AGAGGGACTA 
AATAAAACAG CCATTGGCGA TTACCTCGGC GAGAAAAACG ATTTTAATGA GGATGTGCTT 
AAGGCTTTTG TGGCGCTTCA CGATTTCACC AATCTCATTC TCGTACAAGC CCTCAGACAA 
TTTCTGTGGT CGTTTCGCCT GCCCGGCGAG GCACAAAAGA TCGATCGCAT GATGGAGACC 
TTCGCACAGC GCTACTGTCA ACTAAATCCA GATATATTCA CCAACACGGA CACATGCTAT 
GTGCTCAGCT TCGCAATTAT AATGCTAAAT ACCTCACTAC ACAATCCATC TGTTAAAGAC 
AAACCCACCG TTGATCAATT CATATCGATG AATCGTGGCA TCAACAACGG CGGAGATTTG 
CCCCGTGGCC TGCTGGAATC CCTTTACGAG TCAATTCGAA CAGAGCCATT TAAAATACCA 
CAGGATGATG GAAATGATTT GATGCACACC TTCTTCAATC CCGACAAGGA GGGCTGGCTG 
TGGAAGCAAG GCGGCAGATA CAAATCGTGG AAACGACGTT GGTTCATTTT GAACGATAAT 
TGCTTATACT ATTTTGAATA CACAACGGAT AAGGAACCAC GCGGAATTAT ACCGCTGGAG 
AATATATCGG TGCGCGAGAT TCACGATCGC AGCAAACCGC ATTGCTTTGA GTTGTTTGCG 
ACGGGCGGTG CTGATATAAT CAAGGCATGC AAGACTGACT CTGAGGGCAA AGTGGTAGAG 
GGCAAGCATA CGGTGTACCG CATGTCCGCT GCCACGGAGG AAGATCAGCA GGAGTGGATC 
AAGCGTTTGA CGCAGTCCAT AAGCCATAAT CCGTTTTATG ACATCCTGGT ACAAAGAAAG 
AAAAAGGCAC TCAGCAAGAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260678.1
CDS	1051..3234
Translation	MASLHQKRPSISNWFSSLRRQPKNKKCSPGLGGSESVGRKQPLQRSCLDLSTTRVGCDGCVSLEFFQSESPESRRSNSTFYVNTQNVSSAVFKDNRQPLLERGSGSDSSPSSVCVRCSCNSITKSGDRNETPTMATPKSDIKIALKPPPKPRSPSMAPYTSVATYNITKLAPDSPPPASPTLSTQTVNCSDVTTKVTEVQRLPCDEPNTELISKRTEYKHTTITTTTRTLIVSRPVELLLNENGEIIARRSPRKSRSNSLGRMLDVQNEIGTGGLFMEADPCSKNSLPPPPYIDDMRFIDDSSNSQSESERNSNIHKTNKYTSKNNNNDSKRESQQSNLCESCRMLLDRNYINTEQSDNLVILRRPSKQIKDELCEVVSEMEALDVPEDCKHSNKDKQMSIGRKKFNMDPKKGIEYLVENRLLRHDPQDVAHFLYKGEGLNKTAIGDYLGEKNDFNEDVLKAFVALHDFTNLILVQALRQFLWSFRLPGEAQKIDRMMETFAQRYCQLNPDIFTNTDTCYVLSFAIIMLNTSLHNPSVKDKPTVDQFISMNRGINNGGDLPRGLLESLYESIRTEPFKIPQDDGNDLMHTFFNPDKEGWLWKQGGRYKSWKRRWFILNDNCLYYFEYTTDKEPRGIIPLENISVREIHDRSKPHCFELFATGGADIIKACKTDSEGKVVEGKHTVYRMSAATEEDQQEWIKRLTQSISHNPFYDILVQRKKKALSKS

Target ORF information:

RefSeq Version	NM_001273749.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster steppke (step), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273749.1

ORF Insert Sequence:

ATGGCATCCC TCCATCAGAA GCGTCCCAGC ATAAGTAACT GGTTCTCCTC GCTTCGTCGG 
CAGCCCAAAA ACAAGAAGTG CTCCCCCGGC TTGGGAGGAA GTGAAAGTGT TGGCAGAAAG 
CAGCCATTGC AGAGGAGCTG CCTTGACCTA TCCACGACGC GAGTTGGTTG CGATGGTTGT 
GTAAGTTTGG AGTTCTTTCA ATCGGAATCC CCTGAAAGTC GGCGAAGCAA CAGCACTTTC 
TATGTCAACA CCCAAAATGT ATCGTCTGCT GTTTTTAAAG ACAATCGTCA GCCTCTATTG 
GAAAGGGGCA GCGGTAGTGA TAGTAGCCCT AGCAGCGTTT GCGTTCGATG CTCATGCAAT 
TCGATAACGA AAAGCGGGGA CAGAAATGAA ACACCTACAA TGGCCACGCC TAAGTCGGAC 
ATTAAGATCG CGCTGAAACC TCCACCCAAG CCCCGCTCCC CTTCCATGGC TCCCTACACT 
AGTGTGGCTA CATATAACAT CACGAAATTA GCGCCGGACA GCCCACCCCC CGCCAGCCCC 
ACATTAAGCA CTCAAACCGT AAATTGCAGC GATGTAACCA CGAAGGTCAC AGAGGTACAG 
CGCCTACCCT GCGATGAACC CAACACTGAA CTCATAAGCA AGCGAACGGA GTACAAGCAC 
ACAACCATCA CAACAACGAC AAGAACATTA ATTGTGTCGC GACCCGTGGA ACTGCTTTTA 
AATGAAAATG GCGAAATAAT CGCAAGGCGA TCCCCCCGCA AATCGCGCTC TAATTCCCTG 
GGCCGCATGC TTGACGTGCA GAATGAAATT GGAACTGGCG GGTTGTTTAT GGAAGCGGAT 
CCTTGTTCGA AAAACAGTTT ACCGCCTCCA CCCTATATCG ACGATATGCG CTTCATTGAT 
GACAGCTCCA ACTCACAAAG TGAGTCCGAG CGGAACTCTA ACATACACAA AACCAACAAA 
TATACTTCAA AGAATAATAA CAACGACAGC AAGCGGGAGA GCCAGCAATC GAATCTGTGC 
GAGAGTTGCC GCATGCTTCT CGATCGAAAT TACATAAATA CTGAACAGAG TGACAATCTA 
GTCATCCTCA GGCGACCATC AAAGCAAATT AAAGATGAGT TGTGCGAGGT TGTATCGGAA 
ATGGAGGCGC TGGATGTGCC CGAAGACTGC AAGCATTCCA ATAAAGATAA ACAGATGTCC 
ATTGGCCGTA AAAAGTTTAA CATGGACCCA AAAAAAGGCA TTGAGTATCT CGTTGAAAAC 
AGGCTGCTAC GTCATGATCC CCAGGACGTT GCCCACTTTC TCTATAAGGG AGAGGGACTA 
AATAAAACAG CCATTGGCGA TTACCTCGGC GAGAAAAACG ATTTTAATGA GGATGTGCTT 
AAGGCTTTTG TGGCGCTTCA CGATTTCACC AATCTCATTC TCGTACAAGC CCTCAGACAA 
TTTCTGTGGT CGTTTCGCCT GCCCGGCGAG GCACAAAAGA TCGATCGCAT GATGGAGACC 
TTCGCACAGC GCTACTGTCA ACTAAATCCA GATATATTCA CCAACACGGA CACATGCTAT 
GTGCTCAGCT TCGCAATTAT AATGCTAAAT ACCTCACTAC ACAATCCATC TGTTAAAGAC 
AAACCCACCG TTGATCAATT CATATCGATG AATCGTGGCA TCAACAACGG CGGAGATTTG 
CCCCGTGGCC TGCTGGAATC CCTTTACGAG TCAATTCGAA CAGAGCCATT TAAAATACCA 
CAGGATGATG GAAATGATTT GATGCACACC TTCTTCAATC CCGACAAGGA GGGCTGGCTG 
TGGAAGCAAG GCGGCAGATA CAAATCGTGG AAACGACGTT GGTTCATTTT GAACGATAAT 
TGCTTATACT ATTTTGAATA CACAACGGAT AAGGAACCAC GCGGAATTAT ACCGCTGGAG 
AATATATCGG TGCGCGAGAT TCACGATCGC AGCAAACCGC ATTGCTTTGA GTTGTTTGCG 
ACGGGCGGTG CTGATATAAT CAAGGCATGC AAGACTGACT CTGAGGGCAA AGTGGTAGAG 
GGCAAGCATA CGGTGTACCG CATGTCCGCT GCCACGGAGG AAGATCAGCA GGAGTGGATC 
AAGCGTTTGA CGCAGTCCAT AAGCCATAAT CCGTTTTATG ACATCCTGGT ACAAAGAAAG 
AAAAAGGCAC TCAGCAAGAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

step ( NM_001169570.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163041.1 Drosophila melanogaster steppke (step), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa15147
Clone ID Related Accession (Same CDS sequence)	NM_001273749.1 , NM_001169570.2 , NM_001042910.3
Accession Version	NM_001169570.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	steppke, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001169570.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCCC TCCATCAGAA GCGTCCCAGC ATAAGTAACT GGTTCTCCTC GCTTCGTCGG 
CAGCCCAAAA ACAAGAAGTG CTCCCCCGGC TTGGGAGGAA GTGAAAGTGT TGGCAGAAAG 
CAGCCATTGC AGAGGAGCTG CCTTGACCTA TCCACGACGC GAGTTGGTTG CGATGGTTGT 
GTAAGTTTGG AGTTCTTTCA ATCGGAATCC CCTGAAAGTC GGCGAAGCAA CAGCACTTTC 
TATGTCAACA CCCAAAATGT ATCGTCTGCT GTTTTTAAAG ACAATCGTCA GCCTCTATTG 
GAAAGGGGCA GCGGTAGTGA TAGTAGCCCT AGCAGCGTTT GCGTTCGATG CTCATGCAAT 
TCGATAACGA AAAGCGGGGA CAGAAATGAA ACACCTACAA TGGCCACGCC TAAGTCGGAC 
ATTAAGATCG CGCTGAAACC TCCACCCAAG CCCCGCTCCC CTTCCATGGC TCCCTACACT 
AGTGTGGCTA CATATAACAT CACGAAATTA GCGCCGGACA GCCCACCCCC CGCCAGCCCC 
ACATTAAGCA CTCAAACCGT AAATTGCAGC GATGTAACCA CGAAGGTCAC AGAGGTACAG 
CGCCTACCCT GCGATGAACC CAACACTGAA CTCATAAGCA AGCGAACGGA GTACAAGCAC 
ACAACCATCA CAACAACGAC AAGAACATTA ATTGTGTCGC GACCCGTGGA ACTGCTTTTA 
AATGAAAATG GCGAAATAAT CGCAAGGCGA TCCCCCCGCA AATCGCGCTC TAATTCCCTG 
GGCCGCATGC TTGACGTGCA GAATGAAATT GGAACTGGCG GGTTGTTTAT GGAAGCGGAT 
CCTTGTTCGA AAAACAGTTT ACCGCCTCCA CCCTATATCG ACGATATGCG CTTCATTGAT 
GACAGCTCCA ACTCACAAAG TGAGTCCGAG CGGAACTCTA ACATACACAA AACCAACAAA 
TATACTTCAA AGAATAATAA CAACGACAGC AAGCGGGAGA GCCAGCAATC GAATCTGTGC 
GAGAGTTGCC GCATGCTTCT CGATCGAAAT TACATAAATA CTGAACAGAG TGACAATCTA 
GTCATCCTCA GGCGACCATC AAAGCAAATT AAAGATGAGT TGTGCGAGGT TGTATCGGAA 
ATGGAGGCGC TGGATGTGCC CGAAGACTGC AAGCATTCCA ATAAAGATAA ACAGATGTCC 
ATTGGCCGTA AAAAGTTTAA CATGGACCCA AAAAAAGGCA TTGAGTATCT CGTTGAAAAC 
AGGCTGCTAC GTCATGATCC CCAGGACGTT GCCCACTTTC TCTATAAGGG AGAGGGACTA 
AATAAAACAG CCATTGGCGA TTACCTCGGC GAGAAAAACG ATTTTAATGA GGATGTGCTT 
AAGGCTTTTG TGGCGCTTCA CGATTTCACC AATCTCATTC TCGTACAAGC CCTCAGACAA 
TTTCTGTGGT CGTTTCGCCT GCCCGGCGAG GCACAAAAGA TCGATCGCAT GATGGAGACC 
TTCGCACAGC GCTACTGTCA ACTAAATCCA GATATATTCA CCAACACGGA CACATGCTAT 
GTGCTCAGCT TCGCAATTAT AATGCTAAAT ACCTCACTAC ACAATCCATC TGTTAAAGAC 
AAACCCACCG TTGATCAATT CATATCGATG AATCGTGGCA TCAACAACGG CGGAGATTTG 
CCCCGTGGCC TGCTGGAATC CCTTTACGAG TCAATTCGAA CAGAGCCATT TAAAATACCA 
CAGGATGATG GAAATGATTT GATGCACACC TTCTTCAATC CCGACAAGGA GGGCTGGCTG 
TGGAAGCAAG GCGGCAGATA CAAATCGTGG AAACGACGTT GGTTCATTTT GAACGATAAT 
TGCTTATACT ATTTTGAATA CACAACGGAT AAGGAACCAC GCGGAATTAT ACCGCTGGAG 
AATATATCGG TGCGCGAGAT TCACGATCGC AGCAAACCGC ATTGCTTTGA GTTGTTTGCG 
ACGGGCGGTG CTGATATAAT CAAGGCATGC AAGACTGACT CTGAGGGCAA AGTGGTAGAG 
GGCAAGCATA CGGTGTACCG CATGTCCGCT GCCACGGAGG AAGATCAGCA GGAGTGGATC 
AAGCGTTTGA CGCAGTCCAT AAGCCATAAT CCGTTTTATG ACATCCTGGT ACAAAGAAAG 
AAAAAGGCAC TCAGCAAGAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163041.1
CDS	583..2766
Translation	MASLHQKRPSISNWFSSLRRQPKNKKCSPGLGGSESVGRKQPLQRSCLDLSTTRVGCDGCVSLEFFQSESPESRRSNSTFYVNTQNVSSAVFKDNRQPLLERGSGSDSSPSSVCVRCSCNSITKSGDRNETPTMATPKSDIKIALKPPPKPRSPSMAPYTSVATYNITKLAPDSPPPASPTLSTQTVNCSDVTTKVTEVQRLPCDEPNTELISKRTEYKHTTITTTTRTLIVSRPVELLLNENGEIIARRSPRKSRSNSLGRMLDVQNEIGTGGLFMEADPCSKNSLPPPPYIDDMRFIDDSSNSQSESERNSNIHKTNKYTSKNNNNDSKRESQQSNLCESCRMLLDRNYINTEQSDNLVILRRPSKQIKDELCEVVSEMEALDVPEDCKHSNKDKQMSIGRKKFNMDPKKGIEYLVENRLLRHDPQDVAHFLYKGEGLNKTAIGDYLGEKNDFNEDVLKAFVALHDFTNLILVQALRQFLWSFRLPGEAQKIDRMMETFAQRYCQLNPDIFTNTDTCYVLSFAIIMLNTSLHNPSVKDKPTVDQFISMNRGINNGGDLPRGLLESLYESIRTEPFKIPQDDGNDLMHTFFNPDKEGWLWKQGGRYKSWKRRWFILNDNCLYYFEYTTDKEPRGIIPLENISVREIHDRSKPHCFELFATGGADIIKACKTDSEGKVVEGKHTVYRMSAATEEDQQEWIKRLTQSISHNPFYDILVQRKKKALSKS

Target ORF information:

RefSeq Version	NM_001169570.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster steppke (step), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169570.2

ORF Insert Sequence:

ATGGCATCCC TCCATCAGAA GCGTCCCAGC ATAAGTAACT GGTTCTCCTC GCTTCGTCGG 
CAGCCCAAAA ACAAGAAGTG CTCCCCCGGC TTGGGAGGAA GTGAAAGTGT TGGCAGAAAG 
CAGCCATTGC AGAGGAGCTG CCTTGACCTA TCCACGACGC GAGTTGGTTG CGATGGTTGT 
GTAAGTTTGG AGTTCTTTCA ATCGGAATCC CCTGAAAGTC GGCGAAGCAA CAGCACTTTC 
TATGTCAACA CCCAAAATGT ATCGTCTGCT GTTTTTAAAG ACAATCGTCA GCCTCTATTG 
GAAAGGGGCA GCGGTAGTGA TAGTAGCCCT AGCAGCGTTT GCGTTCGATG CTCATGCAAT 
TCGATAACGA AAAGCGGGGA CAGAAATGAA ACACCTACAA TGGCCACGCC TAAGTCGGAC 
ATTAAGATCG CGCTGAAACC TCCACCCAAG CCCCGCTCCC CTTCCATGGC TCCCTACACT 
AGTGTGGCTA CATATAACAT CACGAAATTA GCGCCGGACA GCCCACCCCC CGCCAGCCCC 
ACATTAAGCA CTCAAACCGT AAATTGCAGC GATGTAACCA CGAAGGTCAC AGAGGTACAG 
CGCCTACCCT GCGATGAACC CAACACTGAA CTCATAAGCA AGCGAACGGA GTACAAGCAC 
ACAACCATCA CAACAACGAC AAGAACATTA ATTGTGTCGC GACCCGTGGA ACTGCTTTTA 
AATGAAAATG GCGAAATAAT CGCAAGGCGA TCCCCCCGCA AATCGCGCTC TAATTCCCTG 
GGCCGCATGC TTGACGTGCA GAATGAAATT GGAACTGGCG GGTTGTTTAT GGAAGCGGAT 
CCTTGTTCGA AAAACAGTTT ACCGCCTCCA CCCTATATCG ACGATATGCG CTTCATTGAT 
GACAGCTCCA ACTCACAAAG TGAGTCCGAG CGGAACTCTA ACATACACAA AACCAACAAA 
TATACTTCAA AGAATAATAA CAACGACAGC AAGCGGGAGA GCCAGCAATC GAATCTGTGC 
GAGAGTTGCC GCATGCTTCT CGATCGAAAT TACATAAATA CTGAACAGAG TGACAATCTA 
GTCATCCTCA GGCGACCATC AAAGCAAATT AAAGATGAGT TGTGCGAGGT TGTATCGGAA 
ATGGAGGCGC TGGATGTGCC CGAAGACTGC AAGCATTCCA ATAAAGATAA ACAGATGTCC 
ATTGGCCGTA AAAAGTTTAA CATGGACCCA AAAAAAGGCA TTGAGTATCT CGTTGAAAAC 
AGGCTGCTAC GTCATGATCC CCAGGACGTT GCCCACTTTC TCTATAAGGG AGAGGGACTA 
AATAAAACAG CCATTGGCGA TTACCTCGGC GAGAAAAACG ATTTTAATGA GGATGTGCTT 
AAGGCTTTTG TGGCGCTTCA CGATTTCACC AATCTCATTC TCGTACAAGC CCTCAGACAA 
TTTCTGTGGT CGTTTCGCCT GCCCGGCGAG GCACAAAAGA TCGATCGCAT GATGGAGACC 
TTCGCACAGC GCTACTGTCA ACTAAATCCA GATATATTCA CCAACACGGA CACATGCTAT 
GTGCTCAGCT TCGCAATTAT AATGCTAAAT ACCTCACTAC ACAATCCATC TGTTAAAGAC 
AAACCCACCG TTGATCAATT CATATCGATG AATCGTGGCA TCAACAACGG CGGAGATTTG 
CCCCGTGGCC TGCTGGAATC CCTTTACGAG TCAATTCGAA CAGAGCCATT TAAAATACCA 
CAGGATGATG GAAATGATTT GATGCACACC TTCTTCAATC CCGACAAGGA GGGCTGGCTG 
TGGAAGCAAG GCGGCAGATA CAAATCGTGG AAACGACGTT GGTTCATTTT GAACGATAAT 
TGCTTATACT ATTTTGAATA CACAACGGAT AAGGAACCAC GCGGAATTAT ACCGCTGGAG 
AATATATCGG TGCGCGAGAT TCACGATCGC AGCAAACCGC ATTGCTTTGA GTTGTTTGCG 
ACGGGCGGTG CTGATATAAT CAAGGCATGC AAGACTGACT CTGAGGGCAA AGTGGTAGAG 
GGCAAGCATA CGGTGTACCG CATGTCCGCT GCCACGGAGG AAGATCAGCA GGAGTGGATC 
AAGCGTTTGA CGCAGTCCAT AAGCCATAAT CCGTTTTATG ACATCCTGGT ACAAAGAAAG 
AAAAAGGCAC TCAGCAAGAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

step ( NM_001042910.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036375.2 Drosophila melanogaster steppke (step), transcript variant C, mRNA.

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CloneID	OFa15147
Clone ID Related Accession (Same CDS sequence)	NM_001273749.1 , NM_001169570.2 , NM_001042910.3
Accession Version	NM_001042910.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	steppke, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001042910.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCCC TCCATCAGAA GCGTCCCAGC ATAAGTAACT GGTTCTCCTC GCTTCGTCGG 
CAGCCCAAAA ACAAGAAGTG CTCCCCCGGC TTGGGAGGAA GTGAAAGTGT TGGCAGAAAG 
CAGCCATTGC AGAGGAGCTG CCTTGACCTA TCCACGACGC GAGTTGGTTG CGATGGTTGT 
GTAAGTTTGG AGTTCTTTCA ATCGGAATCC CCTGAAAGTC GGCGAAGCAA CAGCACTTTC 
TATGTCAACA CCCAAAATGT ATCGTCTGCT GTTTTTAAAG ACAATCGTCA GCCTCTATTG 
GAAAGGGGCA GCGGTAGTGA TAGTAGCCCT AGCAGCGTTT GCGTTCGATG CTCATGCAAT 
TCGATAACGA AAAGCGGGGA CAGAAATGAA ACACCTACAA TGGCCACGCC TAAGTCGGAC 
ATTAAGATCG CGCTGAAACC TCCACCCAAG CCCCGCTCCC CTTCCATGGC TCCCTACACT 
AGTGTGGCTA CATATAACAT CACGAAATTA GCGCCGGACA GCCCACCCCC CGCCAGCCCC 
ACATTAAGCA CTCAAACCGT AAATTGCAGC GATGTAACCA CGAAGGTCAC AGAGGTACAG 
CGCCTACCCT GCGATGAACC CAACACTGAA CTCATAAGCA AGCGAACGGA GTACAAGCAC 
ACAACCATCA CAACAACGAC AAGAACATTA ATTGTGTCGC GACCCGTGGA ACTGCTTTTA 
AATGAAAATG GCGAAATAAT CGCAAGGCGA TCCCCCCGCA AATCGCGCTC TAATTCCCTG 
GGCCGCATGC TTGACGTGCA GAATGAAATT GGAACTGGCG GGTTGTTTAT GGAAGCGGAT 
CCTTGTTCGA AAAACAGTTT ACCGCCTCCA CCCTATATCG ACGATATGCG CTTCATTGAT 
GACAGCTCCA ACTCACAAAG TGAGTCCGAG CGGAACTCTA ACATACACAA AACCAACAAA 
TATACTTCAA AGAATAATAA CAACGACAGC AAGCGGGAGA GCCAGCAATC GAATCTGTGC 
GAGAGTTGCC GCATGCTTCT CGATCGAAAT TACATAAATA CTGAACAGAG TGACAATCTA 
GTCATCCTCA GGCGACCATC AAAGCAAATT AAAGATGAGT TGTGCGAGGT TGTATCGGAA 
ATGGAGGCGC TGGATGTGCC CGAAGACTGC AAGCATTCCA ATAAAGATAA ACAGATGTCC 
ATTGGCCGTA AAAAGTTTAA CATGGACCCA AAAAAAGGCA TTGAGTATCT CGTTGAAAAC 
AGGCTGCTAC GTCATGATCC CCAGGACGTT GCCCACTTTC TCTATAAGGG AGAGGGACTA 
AATAAAACAG CCATTGGCGA TTACCTCGGC GAGAAAAACG ATTTTAATGA GGATGTGCTT 
AAGGCTTTTG TGGCGCTTCA CGATTTCACC AATCTCATTC TCGTACAAGC CCTCAGACAA 
TTTCTGTGGT CGTTTCGCCT GCCCGGCGAG GCACAAAAGA TCGATCGCAT GATGGAGACC 
TTCGCACAGC GCTACTGTCA ACTAAATCCA GATATATTCA CCAACACGGA CACATGCTAT 
GTGCTCAGCT TCGCAATTAT AATGCTAAAT ACCTCACTAC ACAATCCATC TGTTAAAGAC 
AAACCCACCG TTGATCAATT CATATCGATG AATCGTGGCA TCAACAACGG CGGAGATTTG 
CCCCGTGGCC TGCTGGAATC CCTTTACGAG TCAATTCGAA CAGAGCCATT TAAAATACCA 
CAGGATGATG GAAATGATTT GATGCACACC TTCTTCAATC CCGACAAGGA GGGCTGGCTG 
TGGAAGCAAG GCGGCAGATA CAAATCGTGG AAACGACGTT GGTTCATTTT GAACGATAAT 
TGCTTATACT ATTTTGAATA CACAACGGAT AAGGAACCAC GCGGAATTAT ACCGCTGGAG 
AATATATCGG TGCGCGAGAT TCACGATCGC AGCAAACCGC ATTGCTTTGA GTTGTTTGCG 
ACGGGCGGTG CTGATATAAT CAAGGCATGC AAGACTGACT CTGAGGGCAA AGTGGTAGAG 
GGCAAGCATA CGGTGTACCG CATGTCCGCT GCCACGGAGG AAGATCAGCA GGAGTGGATC 
AAGCGTTTGA CGCAGTCCAT AAGCCATAAT CCGTTTTATG ACATCCTGGT ACAAAGAAAG 
AAAAAGGCAC TCAGCAAGAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036375.2
CDS	322..2505
Translation	MASLHQKRPSISNWFSSLRRQPKNKKCSPGLGGSESVGRKQPLQRSCLDLSTTRVGCDGCVSLEFFQSESPESRRSNSTFYVNTQNVSSAVFKDNRQPLLERGSGSDSSPSSVCVRCSCNSITKSGDRNETPTMATPKSDIKIALKPPPKPRSPSMAPYTSVATYNITKLAPDSPPPASPTLSTQTVNCSDVTTKVTEVQRLPCDEPNTELISKRTEYKHTTITTTTRTLIVSRPVELLLNENGEIIARRSPRKSRSNSLGRMLDVQNEIGTGGLFMEADPCSKNSLPPPPYIDDMRFIDDSSNSQSESERNSNIHKTNKYTSKNNNNDSKRESQQSNLCESCRMLLDRNYINTEQSDNLVILRRPSKQIKDELCEVVSEMEALDVPEDCKHSNKDKQMSIGRKKFNMDPKKGIEYLVENRLLRHDPQDVAHFLYKGEGLNKTAIGDYLGEKNDFNEDVLKAFVALHDFTNLILVQALRQFLWSFRLPGEAQKIDRMMETFAQRYCQLNPDIFTNTDTCYVLSFAIIMLNTSLHNPSVKDKPTVDQFISMNRGINNGGDLPRGLLESLYESIRTEPFKIPQDDGNDLMHTFFNPDKEGWLWKQGGRYKSWKRRWFILNDNCLYYFEYTTDKEPRGIIPLENISVREIHDRSKPHCFELFATGGADIIKACKTDSEGKVVEGKHTVYRMSAATEEDQQEWIKRLTQSISHNPFYDILVQRKKKALSKS

Target ORF information:

RefSeq Version	NM_001042910.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster steppke (step), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042910.3

ORF Insert Sequence:

ATGGCATCCC TCCATCAGAA GCGTCCCAGC ATAAGTAACT GGTTCTCCTC GCTTCGTCGG 
CAGCCCAAAA ACAAGAAGTG CTCCCCCGGC TTGGGAGGAA GTGAAAGTGT TGGCAGAAAG 
CAGCCATTGC AGAGGAGCTG CCTTGACCTA TCCACGACGC GAGTTGGTTG CGATGGTTGT 
GTAAGTTTGG AGTTCTTTCA ATCGGAATCC CCTGAAAGTC GGCGAAGCAA CAGCACTTTC 
TATGTCAACA CCCAAAATGT ATCGTCTGCT GTTTTTAAAG ACAATCGTCA GCCTCTATTG 
GAAAGGGGCA GCGGTAGTGA TAGTAGCCCT AGCAGCGTTT GCGTTCGATG CTCATGCAAT 
TCGATAACGA AAAGCGGGGA CAGAAATGAA ACACCTACAA TGGCCACGCC TAAGTCGGAC 
ATTAAGATCG CGCTGAAACC TCCACCCAAG CCCCGCTCCC CTTCCATGGC TCCCTACACT 
AGTGTGGCTA CATATAACAT CACGAAATTA GCGCCGGACA GCCCACCCCC CGCCAGCCCC 
ACATTAAGCA CTCAAACCGT AAATTGCAGC GATGTAACCA CGAAGGTCAC AGAGGTACAG 
CGCCTACCCT GCGATGAACC CAACACTGAA CTCATAAGCA AGCGAACGGA GTACAAGCAC 
ACAACCATCA CAACAACGAC AAGAACATTA ATTGTGTCGC GACCCGTGGA ACTGCTTTTA 
AATGAAAATG GCGAAATAAT CGCAAGGCGA TCCCCCCGCA AATCGCGCTC TAATTCCCTG 
GGCCGCATGC TTGACGTGCA GAATGAAATT GGAACTGGCG GGTTGTTTAT GGAAGCGGAT 
CCTTGTTCGA AAAACAGTTT ACCGCCTCCA CCCTATATCG ACGATATGCG CTTCATTGAT 
GACAGCTCCA ACTCACAAAG TGAGTCCGAG CGGAACTCTA ACATACACAA AACCAACAAA 
TATACTTCAA AGAATAATAA CAACGACAGC AAGCGGGAGA GCCAGCAATC GAATCTGTGC 
GAGAGTTGCC GCATGCTTCT CGATCGAAAT TACATAAATA CTGAACAGAG TGACAATCTA 
GTCATCCTCA GGCGACCATC AAAGCAAATT AAAGATGAGT TGTGCGAGGT TGTATCGGAA 
ATGGAGGCGC TGGATGTGCC CGAAGACTGC AAGCATTCCA ATAAAGATAA ACAGATGTCC 
ATTGGCCGTA AAAAGTTTAA CATGGACCCA AAAAAAGGCA TTGAGTATCT CGTTGAAAAC 
AGGCTGCTAC GTCATGATCC CCAGGACGTT GCCCACTTTC TCTATAAGGG AGAGGGACTA 
AATAAAACAG CCATTGGCGA TTACCTCGGC GAGAAAAACG ATTTTAATGA GGATGTGCTT 
AAGGCTTTTG TGGCGCTTCA CGATTTCACC AATCTCATTC TCGTACAAGC CCTCAGACAA 
TTTCTGTGGT CGTTTCGCCT GCCCGGCGAG GCACAAAAGA TCGATCGCAT GATGGAGACC 
TTCGCACAGC GCTACTGTCA ACTAAATCCA GATATATTCA CCAACACGGA CACATGCTAT 
GTGCTCAGCT TCGCAATTAT AATGCTAAAT ACCTCACTAC ACAATCCATC TGTTAAAGAC 
AAACCCACCG TTGATCAATT CATATCGATG AATCGTGGCA TCAACAACGG CGGAGATTTG 
CCCCGTGGCC TGCTGGAATC CCTTTACGAG TCAATTCGAA CAGAGCCATT TAAAATACCA 
CAGGATGATG GAAATGATTT GATGCACACC TTCTTCAATC CCGACAAGGA GGGCTGGCTG 
TGGAAGCAAG GCGGCAGATA CAAATCGTGG AAACGACGTT GGTTCATTTT GAACGATAAT 
TGCTTATACT ATTTTGAATA CACAACGGAT AAGGAACCAC GCGGAATTAT ACCGCTGGAG 
AATATATCGG TGCGCGAGAT TCACGATCGC AGCAAACCGC ATTGCTTTGA GTTGTTTGCG 
ACGGGCGGTG CTGATATAAT CAAGGCATGC AAGACTGACT CTGAGGGCAA AGTGGTAGAG 
GGCAAGCATA CGGTGTACCG CATGTCCGCT GCCACGGAGG AAGATCAGCA GGAGTGGATC 
AAGCGTTTGA CGCAGTCCAT AAGCCATAAT CCGTTTTATG ACATCCTGGT ACAAAGAAAG 
AAAAAGGCAC TCAGCAAGAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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step cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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