Cul2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cul2
Entrez Gene ID	35420
Full Name	Cullin 2
Synonyms	CG1512,CUL2,Cul-2,Cullin2,DMcul-2,Dmel\CG1512,cul-2,cul2,dCul2,l(2)02074
General protein information	Preferred Names Cullin 2 Names CG1512 gene product from transcript CG1512-RA CG1512-PA CG1512-PB Cul2-PA Cul2-PB Cullin-2 cul-2 cullin 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 39E3-39E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_165390.2	NP_724352.1	cullin 2, isoform A
NM_136273.3	NP_610117.1	cullin 2, isoform B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CUL2	NP_001070377.1
Homo sapiens (human)	CUL2	NP_001185707.1
Canis lupus familiaris (dog)	CUL2	XP_005616994.1
Rattus norvegicus (Norway rat)	Cul2	NP_001101887.1
Gallus gallus (chicken)	CUL2	NP_001026141.1
	CUL2	XP_001087735.2
Danio rerio (zebrafish)	cul2	XP_002666627.1
Drosophila melanogaster (fruit fly)	Cul-2	NP_724352.1
Pan troglodytes (chimpanzee)	CUL2	XP_003312567.1
Mus musculus (house mouse)	Cul2	NP_083678.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003605	XP_313365.4
Caenorhabditis elegans (roundworm)	cul-2	NP_499825.3
Xenopus tropicalis (tropical clawed frog)	cul2	XP_002935209.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

contributes mechanistic insight to a Jak/STAT regulatory genetic circuit, and suggests that Socs36E regulates Jak/STAT signaling via a Cul2-dependent mechanism, as well as by a Cullin-independent manner, in vivo
Title: Socs36E limits STAT signaling via Cullin2 and a SOCS-box independent mechanism in the Drosophila egg chamber.

Cullin-2 protein present in the somatic cells is involved in a non cell-autonomous regulation of the extent of Dpp signaling and thus controls the differentiation of GSCs to cystoblasts
Title: Reduction of Cullin-2 in somatic cells disrupts differentiation of germline stem cells in the Drosophila ovary.

cullin-5 and cullin-2 play a role in the development of neuromuscular junction and the female germ line of Drosophila
Title: Cullin-5 and cullin-2 play a role in the development of neuromuscular junction and the female germ line of Drosophila.

The following Cul2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cul2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15138	NM_136273.3 Latest version!	Drosophila melanogaster cullin 2, transcript variant B (Cul2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15138	NM_165390.2 Latest version!	Drosophila melanogaster cullin 2, transcript variant A (Cul2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cul2 ( NM_165390.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724352.1 Drosophila melanogaster cullin 2, transcript variant A (Cul2), mRNA.

Price & Availability↑

CloneID	OFa15138
Clone ID Related Accession (Same CDS sequence)	NM_165390.2 , NM_136273.3
Accession Version	NM_165390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cullin 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165390.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTCA AGCCCAAAAT AGTGGAGTTT GTCGATGTTT GGCCGCGCCT GCGCTGCATC 
GCCGAGTCGG TGATAACGCT CACCAAAGTG GAGCGCTCGG TGTGGAATAC TAGCTTCAGC 
GACGTTTACA CACTGTGCGT GGCGCAGCCG GAACCGATGG CTGATCGTCT TTATGGCGAA 
ACAAAACACT TTCTTGAGCA GCACGTCCAG GAGATGCTGG CCAAAAAGGT GCTGATCGAG 
GGAGAATGCT CCCATTCCAA TGGTGGCCCA GACCTTCTCC AGCGTTACTA CATCACCTGG 
ATGGAATATA GCCAGGGTAT CAAGTATCTG CACCAATTGT ACATCTACCT GAATCAACAA 
CACATCAAGA AACAAAAGAT TACCGATACG GAATCGTTCT ACGGTAATCT GTCAAGCGAT 
GCTGCGGAGC AGATGGAGAT TGGCGAGCTA GGTCTAGACA TTTGGCGCCT GTATATGATT 
GAGTACTTGT CCAGCGAACT GGTCCGTCAC ATACTCGAGG GCATTGCCGC AGACAGAGCT 
AGCAATGGCA CCTTGGATCA CCATCGCGTG CAAATTATCA ACGGAGTGAT ACACAGCTTC 
GTGGAGGTAC AGGACTATAA GAAAACAGGC TCGCTTAAGC TCTACCAGGA ACTCTTTGAA 
GGCCCCATGC TGGAGGCCAG TGGCGCCTAC TACACAGACG AGGCTAACAA GTTGCTCCAT 
CGGTGCTCAG TGTCGGAGTA CATGCAGGAG GTTATCCGGA TACTGGAATA TGAAAGCCGG 
AGAGCCCAAA AGTTTTTACA CGTCAGTTCC CTGCCAAAAC TACGTAAAGA GTGCGAGGAG 
AAGTTTATTA ACGATCGTTT GGGCTTCATC TACTCGGAGT GCCGCGAAAT GGTTTCAGAG 
GAGCGGCGCC AAGACTTACG CAACATGTAC GTCGTACTAA AGCCCATACC CGATAATCTC 
AAGTCTGAGC TCATAACCAC CTTCCTCGAT CACATCAAAA GTGAAGGTCT GCAGACCGTA 
TCTGCGCTGA AGGGCGAAAA CATCCATATT GCGTTCGTAG AGAATATGTT GAAGGTACAT 
CACAAGTACC AAGAGCTGAT CGCAGACGTC TTCGAAAACG ATTCTCTATT CCTGAGCGCA 
TTAGACAAGG CGTGCGCCAG CGTTATCAAT CGCCGGCCAA CCGAGCGGCA GCCTTGCCGT 
AGTGCCGAGT ATGTGGCGAA ATACTGTGAT ACGCTACTGA AAAAGTCTAA GACCTGCGAG 
GCAGAGATCG ATCAAAAGCT TACTAACAAC ATAACCATAT TTAAATATAT CGAGGATAAG 
GATGTGTATC AAAAGTTTTA TAGCCGACTG TTAGCCAAGC GCCTAATCCA CGAGCAAAGT 
CAGAGCATGG ATGCGGAGGA GGGAATGATC AATCGTTTGA AGCAAGCCTG CGGTTATGAA 
TTTACCAATA AACTTCATCG CATGTTTACG GATATATCGG TATCAGTAGA TCTGAACAAC 
AAGTTTAATA CTCACCTTAA GGATAGCAAT GTGGACTTAG GCATCAACCT TGCCATTAAG 
GTACTGCAGG CGGGCGCGTG GCCCTTGGGA TCTACTCAAG TTATACCATT TGCAGTACCA 
CAGGAATTTG AAAAATCTAT TAAAATGTTC GAGGATTACT ATCATAAATT ATTTAGTGGC 
CGGAAGTTGA CTTGGCTACA TCACATGTGT CATGGAGAAT TAAAGTTGAG CCATTTGAAA 
AAATCTTATA TCGTGACTAT GCAAACATAC CAGATGGCGA TAATACTGCT GTTCGAGACT 
TGTGATAGTC TGAGTTGTCG GGAGATCCAG AACACCTTAC AGCTAAACGA CGAAACGTTT 
CAAAAGCACA TGCAGCCTAT TATCGAGTCA AAGCTTTTAA ATGCCAGCTC AGAGAATCTC 
GCCGGTGAGA CTCGAATAGA ATTAAATTTG GATTACACAA ATAAGCGTAC AAAGTTTAAA 
ATAAGTTCGG CTTTGCAAAA AGAAACGCCA CAGGAGGTTG AGCACACAAT TAACTCGGTA 
GATGAGGACC GAAAACTCTT TCTACAAGCC GCCATTGTAA GGATTATGAA AGCGCGTAAA 
GTCTTAAAGC ACAATGCCTT AATACAAGAG GTTCTCTCTT TATCGAAGGT TAGCTTTACT 
CCCAACATAG CAATGATCAA GAAATGCGTG GAGTCATTAA TTGACAAACA ATATATTGAG 
CGAACGGCGA ACTCGGGCGA TGAATATAGC TACATGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724352.1
CDS	287..2548
Translation	MSLKPKIVEFVDVWPRLRCIAESVITLTKVERSVWNTSFSDVYTLCVAQPEPMADRLYGETKHFLEQHVQEMLAKKVLIEGECSHSNGGPDLLQRYYITWMEYSQGIKYLHQLYIYLNQQHIKKQKITDTESFYGNLSSDAAEQMEIGELGLDIWRLYMIEYLSSELVRHILEGIAADRASNGTLDHHRVQIINGVIHSFVEVQDYKKTGSLKLYQELFEGPMLEASGAYYTDEANKLLHRCSVSEYMQEVIRILEYESRRAQKFLHVSSLPKLRKECEEKFINDRLGFIYSECREMVSEERRQDLRNMYVVLKPIPDNLKSELITTFLDHIKSEGLQTVSALKGENIHIAFVENMLKVHHKYQELIADVFENDSLFLSALDKACASVINRRPTERQPCRSAEYVAKYCDTLLKKSKTCEAEIDQKLTNNITIFKYIEDKDVYQKFYSRLLAKRLIHEQSQSMDAEEGMINRLKQACGYEFTNKLHRMFTDISVSVDLNNKFNTHLKDSNVDLGINLAIKVLQAGAWPLGSTQVIPFAVPQEFEKSIKMFEDYYHKLFSGRKLTWLHHMCHGELKLSHLKKSYIVTMQTYQMAIILLFETCDSLSCREIQNTLQLNDETFQKHMQPIIESKLLNASSENLAGETRIELNLDYTNKRTKFKISSALQKETPQEVEHTINSVDEDRKLFLQAAIVRIMKARKVLKHNALIQEVLSLSKVSFTPNIAMIKKCVESLIDKQYIERTANSGDEYSYMA

Target ORF information:

RefSeq Version	NM_165390.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cullin 2, transcript variant A (Cul2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165390.2

ORF Insert Sequence:

ATGTCGCTCA AGCCCAAAAT AGTGGAGTTT GTCGATGTTT GGCCGCGCCT GCGCTGCATC 
GCCGAGTCGG TGATAACGCT CACCAAAGTG GAGCGCTCGG TGTGGAATAC TAGCTTCAGC 
GACGTTTACA CACTGTGCGT GGCGCAGCCG GAACCGATGG CTGATCGTCT TTATGGCGAA 
ACAAAACACT TTCTTGAGCA GCACGTCCAG GAGATGCTGG CCAAAAAGGT GCTGATCGAG 
GGAGAATGCT CCCATTCCAA TGGTGGCCCA GACCTTCTCC AGCGTTACTA CATCACCTGG 
ATGGAATATA GCCAGGGTAT CAAGTATCTG CACCAATTGT ACATCTACCT GAATCAACAA 
CACATCAAGA AACAAAAGAT TACCGATACG GAATCGTTCT ACGGTAATCT GTCAAGCGAT 
GCTGCGGAGC AGATGGAGAT TGGCGAGCTA GGTCTAGACA TTTGGCGCCT GTATATGATT 
GAGTACTTGT CCAGCGAACT GGTCCGTCAC ATACTCGAGG GCATTGCCGC AGACAGAGCT 
AGCAATGGCA CCTTGGATCA CCATCGCGTG CAAATTATCA ACGGAGTGAT ACACAGCTTC 
GTGGAGGTAC AGGACTATAA GAAAACAGGC TCGCTTAAGC TCTACCAGGA ACTCTTTGAA 
GGCCCCATGC TGGAGGCCAG TGGCGCCTAC TACACAGACG AGGCTAACAA GTTGCTCCAT 
CGGTGCTCAG TGTCGGAGTA CATGCAGGAG GTTATCCGGA TACTGGAATA TGAAAGCCGG 
AGAGCCCAAA AGTTTTTACA CGTCAGTTCC CTGCCAAAAC TACGTAAAGA GTGCGAGGAG 
AAGTTTATTA ACGATCGTTT GGGCTTCATC TACTCGGAGT GCCGCGAAAT GGTTTCAGAG 
GAGCGGCGCC AAGACTTACG CAACATGTAC GTCGTACTAA AGCCCATACC CGATAATCTC 
AAGTCTGAGC TCATAACCAC CTTCCTCGAT CACATCAAAA GTGAAGGTCT GCAGACCGTA 
TCTGCGCTGA AGGGCGAAAA CATCCATATT GCGTTCGTAG AGAATATGTT GAAGGTACAT 
CACAAGTACC AAGAGCTGAT CGCAGACGTC TTCGAAAACG ATTCTCTATT CCTGAGCGCA 
TTAGACAAGG CGTGCGCCAG CGTTATCAAT CGCCGGCCAA CCGAGCGGCA GCCTTGCCGT 
AGTGCCGAGT ATGTGGCGAA ATACTGTGAT ACGCTACTGA AAAAGTCTAA GACCTGCGAG 
GCAGAGATCG ATCAAAAGCT TACTAACAAC ATAACCATAT TTAAATATAT CGAGGATAAG 
GATGTGTATC AAAAGTTTTA TAGCCGACTG TTAGCCAAGC GCCTAATCCA CGAGCAAAGT 
CAGAGCATGG ATGCGGAGGA GGGAATGATC AATCGTTTGA AGCAAGCCTG CGGTTATGAA 
TTTACCAATA AACTTCATCG CATGTTTACG GATATATCGG TATCAGTAGA TCTGAACAAC 
AAGTTTAATA CTCACCTTAA GGATAGCAAT GTGGACTTAG GCATCAACCT TGCCATTAAG 
GTACTGCAGG CGGGCGCGTG GCCCTTGGGA TCTACTCAAG TTATACCATT TGCAGTACCA 
CAGGAATTTG AAAAATCTAT TAAAATGTTC GAGGATTACT ATCATAAATT ATTTAGTGGC 
CGGAAGTTGA CTTGGCTACA TCACATGTGT CATGGAGAAT TAAAGTTGAG CCATTTGAAA 
AAATCTTATA TCGTGACTAT GCAAACATAC CAGATGGCGA TAATACTGCT GTTCGAGACT 
TGTGATAGTC TGAGTTGTCG GGAGATCCAG AACACCTTAC AGCTAAACGA CGAAACGTTT 
CAAAAGCACA TGCAGCCTAT TATCGAGTCA AAGCTTTTAA ATGCCAGCTC AGAGAATCTC 
GCCGGTGAGA CTCGAATAGA ATTAAATTTG GATTACACAA ATAAGCGTAC AAAGTTTAAA 
ATAAGTTCGG CTTTGCAAAA AGAAACGCCA CAGGAGGTTG AGCACACAAT TAACTCGGTA 
GATGAGGACC GAAAACTCTT TCTACAAGCC GCCATTGTAA GGATTATGAA AGCGCGTAAA 
GTCTTAAAGC ACAATGCCTT AATACAAGAG GTTCTCTCTT TATCGAAGGT TAGCTTTACT 
CCCAACATAG CAATGATCAA GAAATGCGTG GAGTCATTAA TTGACAAACA ATATATTGAG 
CGAACGGCGA ACTCGGGCGA TGAATATAGC TACATGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul2 ( NM_136273.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610117.1 Drosophila melanogaster cullin 2, transcript variant B (Cul2), mRNA.

Price & Availability↑

CloneID	OFa15138
Clone ID Related Accession (Same CDS sequence)	NM_165390.2 , NM_136273.3
Accession Version	NM_136273.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cullin 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_136273.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTCA AGCCCAAAAT AGTGGAGTTT GTCGATGTTT GGCCGCGCCT GCGCTGCATC 
GCCGAGTCGG TGATAACGCT CACCAAAGTG GAGCGCTCGG TGTGGAATAC TAGCTTCAGC 
GACGTTTACA CACTGTGCGT GGCGCAGCCG GAACCGATGG CTGATCGTCT TTATGGCGAA 
ACAAAACACT TTCTTGAGCA GCACGTCCAG GAGATGCTGG CCAAAAAGGT GCTGATCGAG 
GGAGAATGCT CCCATTCCAA TGGTGGCCCA GACCTTCTCC AGCGTTACTA CATCACCTGG 
ATGGAATATA GCCAGGGTAT CAAGTATCTG CACCAATTGT ACATCTACCT GAATCAACAA 
CACATCAAGA AACAAAAGAT TACCGATACG GAATCGTTCT ACGGTAATCT GTCAAGCGAT 
GCTGCGGAGC AGATGGAGAT TGGCGAGCTA GGTCTAGACA TTTGGCGCCT GTATATGATT 
GAGTACTTGT CCAGCGAACT GGTCCGTCAC ATACTCGAGG GCATTGCCGC AGACAGAGCT 
AGCAATGGCA CCTTGGATCA CCATCGCGTG CAAATTATCA ACGGAGTGAT ACACAGCTTC 
GTGGAGGTAC AGGACTATAA GAAAACAGGC TCGCTTAAGC TCTACCAGGA ACTCTTTGAA 
GGCCCCATGC TGGAGGCCAG TGGCGCCTAC TACACAGACG AGGCTAACAA GTTGCTCCAT 
CGGTGCTCAG TGTCGGAGTA CATGCAGGAG GTTATCCGGA TACTGGAATA TGAAAGCCGG 
AGAGCCCAAA AGTTTTTACA CGTCAGTTCC CTGCCAAAAC TACGTAAAGA GTGCGAGGAG 
AAGTTTATTA ACGATCGTTT GGGCTTCATC TACTCGGAGT GCCGCGAAAT GGTTTCAGAG 
GAGCGGCGCC AAGACTTACG CAACATGTAC GTCGTACTAA AGCCCATACC CGATAATCTC 
AAGTCTGAGC TCATAACCAC CTTCCTCGAT CACATCAAAA GTGAAGGTCT GCAGACCGTA 
TCTGCGCTGA AGGGCGAAAA CATCCATATT GCGTTCGTAG AGAATATGTT GAAGGTACAT 
CACAAGTACC AAGAGCTGAT CGCAGACGTC TTCGAAAACG ATTCTCTATT CCTGAGCGCA 
TTAGACAAGG CGTGCGCCAG CGTTATCAAT CGCCGGCCAA CCGAGCGGCA GCCTTGCCGT 
AGTGCCGAGT ATGTGGCGAA ATACTGTGAT ACGCTACTGA AAAAGTCTAA GACCTGCGAG 
GCAGAGATCG ATCAAAAGCT TACTAACAAC ATAACCATAT TTAAATATAT CGAGGATAAG 
GATGTGTATC AAAAGTTTTA TAGCCGACTG TTAGCCAAGC GCCTAATCCA CGAGCAAAGT 
CAGAGCATGG ATGCGGAGGA GGGAATGATC AATCGTTTGA AGCAAGCCTG CGGTTATGAA 
TTTACCAATA AACTTCATCG CATGTTTACG GATATATCGG TATCAGTAGA TCTGAACAAC 
AAGTTTAATA CTCACCTTAA GGATAGCAAT GTGGACTTAG GCATCAACCT TGCCATTAAG 
GTACTGCAGG CGGGCGCGTG GCCCTTGGGA TCTACTCAAG TTATACCATT TGCAGTACCA 
CAGGAATTTG AAAAATCTAT TAAAATGTTC GAGGATTACT ATCATAAATT ATTTAGTGGC 
CGGAAGTTGA CTTGGCTACA TCACATGTGT CATGGAGAAT TAAAGTTGAG CCATTTGAAA 
AAATCTTATA TCGTGACTAT GCAAACATAC CAGATGGCGA TAATACTGCT GTTCGAGACT 
TGTGATAGTC TGAGTTGTCG GGAGATCCAG AACACCTTAC AGCTAAACGA CGAAACGTTT 
CAAAAGCACA TGCAGCCTAT TATCGAGTCA AAGCTTTTAA ATGCCAGCTC AGAGAATCTC 
GCCGGTGAGA CTCGAATAGA ATTAAATTTG GATTACACAA ATAAGCGTAC AAAGTTTAAA 
ATAAGTTCGG CTTTGCAAAA AGAAACGCCA CAGGAGGTTG AGCACACAAT TAACTCGGTA 
GATGAGGACC GAAAACTCTT TCTACAAGCC GCCATTGTAA GGATTATGAA AGCGCGTAAA 
GTCTTAAAGC ACAATGCCTT AATACAAGAG GTTCTCTCTT TATCGAAGGT TAGCTTTACT 
CCCAACATAG CAATGATCAA GAAATGCGTG GAGTCATTAA TTGACAAACA ATATATTGAG 
CGAACGGCGA ACTCGGGCGA TGAATATAGC TACATGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610117.1
CDS	180..2441
Translation	MSLKPKIVEFVDVWPRLRCIAESVITLTKVERSVWNTSFSDVYTLCVAQPEPMADRLYGETKHFLEQHVQEMLAKKVLIEGECSHSNGGPDLLQRYYITWMEYSQGIKYLHQLYIYLNQQHIKKQKITDTESFYGNLSSDAAEQMEIGELGLDIWRLYMIEYLSSELVRHILEGIAADRASNGTLDHHRVQIINGVIHSFVEVQDYKKTGSLKLYQELFEGPMLEASGAYYTDEANKLLHRCSVSEYMQEVIRILEYESRRAQKFLHVSSLPKLRKECEEKFINDRLGFIYSECREMVSEERRQDLRNMYVVLKPIPDNLKSELITTFLDHIKSEGLQTVSALKGENIHIAFVENMLKVHHKYQELIADVFENDSLFLSALDKACASVINRRPTERQPCRSAEYVAKYCDTLLKKSKTCEAEIDQKLTNNITIFKYIEDKDVYQKFYSRLLAKRLIHEQSQSMDAEEGMINRLKQACGYEFTNKLHRMFTDISVSVDLNNKFNTHLKDSNVDLGINLAIKVLQAGAWPLGSTQVIPFAVPQEFEKSIKMFEDYYHKLFSGRKLTWLHHMCHGELKLSHLKKSYIVTMQTYQMAIILLFETCDSLSCREIQNTLQLNDETFQKHMQPIIESKLLNASSENLAGETRIELNLDYTNKRTKFKISSALQKETPQEVEHTINSVDEDRKLFLQAAIVRIMKARKVLKHNALIQEVLSLSKVSFTPNIAMIKKCVESLIDKQYIERTANSGDEYSYMA

Target ORF information:

RefSeq Version	NM_136273.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cullin 2, transcript variant B (Cul2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136273.3

ORF Insert Sequence:

ATGTCGCTCA AGCCCAAAAT AGTGGAGTTT GTCGATGTTT GGCCGCGCCT GCGCTGCATC 
GCCGAGTCGG TGATAACGCT CACCAAAGTG GAGCGCTCGG TGTGGAATAC TAGCTTCAGC 
GACGTTTACA CACTGTGCGT GGCGCAGCCG GAACCGATGG CTGATCGTCT TTATGGCGAA 
ACAAAACACT TTCTTGAGCA GCACGTCCAG GAGATGCTGG CCAAAAAGGT GCTGATCGAG 
GGAGAATGCT CCCATTCCAA TGGTGGCCCA GACCTTCTCC AGCGTTACTA CATCACCTGG 
ATGGAATATA GCCAGGGTAT CAAGTATCTG CACCAATTGT ACATCTACCT GAATCAACAA 
CACATCAAGA AACAAAAGAT TACCGATACG GAATCGTTCT ACGGTAATCT GTCAAGCGAT 
GCTGCGGAGC AGATGGAGAT TGGCGAGCTA GGTCTAGACA TTTGGCGCCT GTATATGATT 
GAGTACTTGT CCAGCGAACT GGTCCGTCAC ATACTCGAGG GCATTGCCGC AGACAGAGCT 
AGCAATGGCA CCTTGGATCA CCATCGCGTG CAAATTATCA ACGGAGTGAT ACACAGCTTC 
GTGGAGGTAC AGGACTATAA GAAAACAGGC TCGCTTAAGC TCTACCAGGA ACTCTTTGAA 
GGCCCCATGC TGGAGGCCAG TGGCGCCTAC TACACAGACG AGGCTAACAA GTTGCTCCAT 
CGGTGCTCAG TGTCGGAGTA CATGCAGGAG GTTATCCGGA TACTGGAATA TGAAAGCCGG 
AGAGCCCAAA AGTTTTTACA CGTCAGTTCC CTGCCAAAAC TACGTAAAGA GTGCGAGGAG 
AAGTTTATTA ACGATCGTTT GGGCTTCATC TACTCGGAGT GCCGCGAAAT GGTTTCAGAG 
GAGCGGCGCC AAGACTTACG CAACATGTAC GTCGTACTAA AGCCCATACC CGATAATCTC 
AAGTCTGAGC TCATAACCAC CTTCCTCGAT CACATCAAAA GTGAAGGTCT GCAGACCGTA 
TCTGCGCTGA AGGGCGAAAA CATCCATATT GCGTTCGTAG AGAATATGTT GAAGGTACAT 
CACAAGTACC AAGAGCTGAT CGCAGACGTC TTCGAAAACG ATTCTCTATT CCTGAGCGCA 
TTAGACAAGG CGTGCGCCAG CGTTATCAAT CGCCGGCCAA CCGAGCGGCA GCCTTGCCGT 
AGTGCCGAGT ATGTGGCGAA ATACTGTGAT ACGCTACTGA AAAAGTCTAA GACCTGCGAG 
GCAGAGATCG ATCAAAAGCT TACTAACAAC ATAACCATAT TTAAATATAT CGAGGATAAG 
GATGTGTATC AAAAGTTTTA TAGCCGACTG TTAGCCAAGC GCCTAATCCA CGAGCAAAGT 
CAGAGCATGG ATGCGGAGGA GGGAATGATC AATCGTTTGA AGCAAGCCTG CGGTTATGAA 
TTTACCAATA AACTTCATCG CATGTTTACG GATATATCGG TATCAGTAGA TCTGAACAAC 
AAGTTTAATA CTCACCTTAA GGATAGCAAT GTGGACTTAG GCATCAACCT TGCCATTAAG 
GTACTGCAGG CGGGCGCGTG GCCCTTGGGA TCTACTCAAG TTATACCATT TGCAGTACCA 
CAGGAATTTG AAAAATCTAT TAAAATGTTC GAGGATTACT ATCATAAATT ATTTAGTGGC 
CGGAAGTTGA CTTGGCTACA TCACATGTGT CATGGAGAAT TAAAGTTGAG CCATTTGAAA 
AAATCTTATA TCGTGACTAT GCAAACATAC CAGATGGCGA TAATACTGCT GTTCGAGACT 
TGTGATAGTC TGAGTTGTCG GGAGATCCAG AACACCTTAC AGCTAAACGA CGAAACGTTT 
CAAAAGCACA TGCAGCCTAT TATCGAGTCA AAGCTTTTAA ATGCCAGCTC AGAGAATCTC 
GCCGGTGAGA CTCGAATAGA ATTAAATTTG GATTACACAA ATAAGCGTAC AAAGTTTAAA 
ATAAGTTCGG CTTTGCAAAA AGAAACGCCA CAGGAGGTTG AGCACACAAT TAACTCGGTA 
GATGAGGACC GAAAACTCTT TCTACAAGCC GCCATTGTAA GGATTATGAA AGCGCGTAAA 
GTCTTAAAGC ACAATGCCTT AATACAAGAG GTTCTCTCTT TATCGAAGGT TAGCTTTACT 
CCCAACATAG CAATGATCAA GAAATGCGTG GAGTCATTAA TTGACAAACA ATATATTGAG 
CGAACGGCGA ACTCGGGCGA TGAATATAGC TACATGGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cul2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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