E2f2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	E2f2
Entrez Gene ID	35381
Full Name	E2F transcription factor 2
Synonyms	CG1071,Dmel\CG1071,E2F,E2F2,E2f,dE2F-2,dE2F2,dE2f2,de2f2,e2f2
General protein information	Preferred Names E2F transcription factor 2 Names CG1071 gene product from transcript CG1071-RB CG1071-PA CG1071-PB E2f2-PA E2f2-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 39A5-39A6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299186.1	NP_001286115.1	E2F transcription factor 2, isoform B
NM_058007.5	NP_477355.1	E2F transcription factor 2, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	E2F5	XP_001169141.1
Danio rerio (zebrafish)	e2f5	NP_001184229.1
Arabidopsis thaliana (thale cress)	E2F1	NP_001031921.3
Rattus norvegicus (Norway rat)	E2f5	XP_574892.1
Drosophila melanogaster (fruit fly)	E2f2	NP_477355.1
Bos taurus (cattle)	E2F5	XP_002692876.1
Mus musculus (house mouse)	E2f5	NP_031918.2
Gallus gallus (chicken)	E2F5	NP_001026113.1
Xenopus tropicalis (tropical clawed frog)	e2f5	NP_001120880.1
Homo sapiens (human)	E2F5	NP_001942.2
	E2F5	XP_001094919.1
Canis lupus familiaris (dog)	E2F5	XP_005638211.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

E2F directly regulates the expression of late myogenic genes, with E2f1 providing the major contribution compared with E2f2.
Title: E2F function in muscle growth is necessary and sufficient for viability in Drosophila.

The dE2F2 protein repress the expression of buffy, the anti-apoptotic member of the Bcl-2 family and activate the expression of an RNA-binding protein that destabilizes diap1 in Drosophila.
Title: The pro-apoptotic activity of Drosophila Rbf1 involves dE2F2-dependent downregulation of diap1 and buffy mRNA.

Heterozygous mutation of mip120 or E2f2 suppressed the binucleate cell phenotype in the peripodial epithelium of Myb-null wing discs.
Title: The complex containing Drosophila Myb and RB/E2F2 regulates cytokinesis in a histone H2Av-dependent manner.

dE2F2, as well as the net E2F activity, which can be depleted by mutating the common cofactor, dDp, is inhibitory for p53-independent apoptosis.
Title: E2F1 and E2F2 have opposite effects on radiation-induced p53-independent apoptosis in Drosophila.

Combined inactivation of dE2F1 and dE2F2 had no significant effect on cell division of Hippo pathway mutant cells.
Title: Context-dependent requirement for dE2F during oncogenic proliferation.

The following E2f2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E2f2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15101	NM_058007.5 Latest version!	Drosophila melanogaster E2F transcription factor 2 (E2f2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa15101	NM_001299186.1 Latest version!	Drosophila melanogaster E2F transcription factor 2 (E2f2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E2f2 ( NM_001299186.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286115.1 Drosophila melanogaster E2F transcription factor 2 (E2f2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15101
Clone ID Related Accession (Same CDS sequence)	NM_001299186.1 , NM_058007.5
Accession Version	NM_001299186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	E2F transcription factor 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACAAGC GCAAAACCGC GAGTATTGTT AAAAGAGACA GCTCCGCAGC GGGCACCACC 
TCCTCGGCTA TGATGATGAA GGTGGATTCG GCTGAGACTT CGGTCCGGTC GCAGAGCTAC 
GAGTCTACAC CCGTTAGCAT GGACACATCA CCGGATCCTC CAACGCCAAT CAAGTCTCCG 
TCGAATTCAC AATCGCAATC GCAGCCTGGA CAACAGCGCT CCGTGGGCTC ACTGGTCCTG 
CTCACACAGA AGTTTGTGGA TCTCGTGAAG GCCAACGAAG GATCCATCGA CCTGAAAGCG 
GCAACCAAAA TCTTGGACGT ACAGAAGCGC CGAATATACG ATATTACCAA TGTTTTAGAG 
GGCATTGGAC TAATTGATAA GGGCAGACAC TGCTCCCTAG TGCGCTGGCG CGGAGGGGGC 
TTTAACAATG CCAAGGACCA AGAGAACTAC GACCTGGCAC GTAGCCGGAC TAATCATTTG 
AAAATGTTGG AGGATGACCT AGACAGGCAA CTGGAGTATG CACAGCGCAA TCTGCGCTAC 
GTTATGCAGG ATCCCTCGAA TAGGTCGTAT GCATATGTGA CACGTGATGA TCTGCTGGAC 
ATCTTTGGAG ATGATTCCGT ATTCACAATA CCTAATTATG ACGAGGAAGT AGATATCAAG 
CGTAATCATT ACGAGCTGGC CGTGTCGCTG GACAATGGCA GCGCAATTGA CATTCGCCTG 
GTGACGAACC AAGGAAAGAG TACTACAAAT CCGCACGATG TGGATGGGTT CTTTGACTAT 
CACCGTCTGG ACACGCCCTC ACCCTCGACG TCGTCGCACT CCAGCGAGGA TGGTAACGCT 
CCAGCATGCG CGGGGAACGT GATCACCGAC GAGCACGGTT ACTCGTGCAA TCCCGGGATG 
AAAGATGAGA TGAAACTTTT GGAGAACGAG CTGACGGCCA AGATAATCTT CCAAAATTAT 
CTGTCCGGTC ATTCGCTGCG GCGATTTTAT CCCGATGATC CGAATCTAGA AAACCCGCCG 
CTGCTGCAGC TGAATCCTCC GCAGGAAGAC TTCAACTTTG CGTTAAAAAG CGACGAAGGT 
ATTTGCGAGC TGTTTGATGT TCAGTGCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286115.1
CDS	707..1819
Translation	MYKRKTASIVKRDSSAAGTTSSAMMMKVDSAETSVRSQSYESTPVSMDTSPDPPTPIKSPSNSQSQSQPGQQRSVGSLVLLTQKFVDLVKANEGSIDLKAATKILDVQKRRIYDITNVLEGIGLIDKGRHCSLVRWRGGGFNNAKDQENYDLARSRTNHLKMLEDDLDRQLEYAQRNLRYVMQDPSNRSYAYVTRDDLLDIFGDDSVFTIPNYDEEVDIKRNHYELAVSLDNGSAIDIRLVTNQGKSTTNPHDVDGFFDYHRLDTPSPSTSSHSSEDGNAPACAGNVITDEHGYSCNPGMKDEMKLLENELTAKIIFQNYLSGHSLRRFYPDDPNLENPPLLQLNPPQEDFNFALKSDEGICELFDVQCS

Target ORF information:

RefSeq Version	NM_001299186.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster E2F transcription factor 2 (E2f2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299186.1

ORF Insert Sequence:

ATGTACAAGC GCAAAACCGC GAGTATTGTT AAAAGAGACA GCTCCGCAGC GGGCACCACC 
TCCTCGGCTA TGATGATGAA GGTGGATTCG GCTGAGACTT CGGTCCGGTC GCAGAGCTAC 
GAGTCTACAC CCGTTAGCAT GGACACATCA CCGGATCCTC CAACGCCAAT CAAGTCTCCG 
TCGAATTCAC AATCGCAATC GCAGCCTGGA CAACAGCGCT CCGTGGGCTC ACTGGTCCTG 
CTCACACAGA AGTTTGTGGA TCTCGTGAAG GCCAACGAAG GATCCATCGA CCTGAAAGCG 
GCAACCAAAA TCTTGGACGT ACAGAAGCGC CGAATATACG ATATTACCAA TGTTTTAGAG 
GGCATTGGAC TAATTGATAA GGGCAGACAC TGCTCCCTAG TGCGCTGGCG CGGAGGGGGC 
TTTAACAATG CCAAGGACCA AGAGAACTAC GACCTGGCAC GTAGCCGGAC TAATCATTTG 
AAAATGTTGG AGGATGACCT AGACAGGCAA CTGGAGTATG CACAGCGCAA TCTGCGCTAC 
GTTATGCAGG ATCCCTCGAA TAGGTCGTAT GCATATGTGA CACGTGATGA TCTGCTGGAC 
ATCTTTGGAG ATGATTCCGT ATTCACAATA CCTAATTATG ACGAGGAAGT AGATATCAAG 
CGTAATCATT ACGAGCTGGC CGTGTCGCTG GACAATGGCA GCGCAATTGA CATTCGCCTG 
GTGACGAACC AAGGAAAGAG TACTACAAAT CCGCACGATG TGGATGGGTT CTTTGACTAT 
CACCGTCTGG ACACGCCCTC ACCCTCGACG TCGTCGCACT CCAGCGAGGA TGGTAACGCT 
CCAGCATGCG CGGGGAACGT GATCACCGAC GAGCACGGTT ACTCGTGCAA TCCCGGGATG 
AAAGATGAGA TGAAACTTTT GGAGAACGAG CTGACGGCCA AGATAATCTT CCAAAATTAT 
CTGTCCGGTC ATTCGCTGCG GCGATTTTAT CCCGATGATC CGAATCTAGA AAACCCGCCG 
CTGCTGCAGC TGAATCCTCC GCAGGAAGAC TTCAACTTTG CGTTAAAAAG CGACGAAGGT 
ATTTGCGAGC TGTTTGATGT TCAGTGCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E2f2 ( NM_058007.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477355.1 Drosophila melanogaster E2F transcription factor 2 (E2f2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15101
Clone ID Related Accession (Same CDS sequence)	NM_001299186.1 , NM_058007.5
Accession Version	NM_058007.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	E2F transcription factor 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_058007.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACAAGC GCAAAACCGC GAGTATTGTT AAAAGAGACA GCTCCGCAGC GGGCACCACC 
TCCTCGGCTA TGATGATGAA GGTGGATTCG GCTGAGACTT CGGTCCGGTC GCAGAGCTAC 
GAGTCTACAC CCGTTAGCAT GGACACATCA CCGGATCCTC CAACGCCAAT CAAGTCTCCG 
TCGAATTCAC AATCGCAATC GCAGCCTGGA CAACAGCGCT CCGTGGGCTC ACTGGTCCTG 
CTCACACAGA AGTTTGTGGA TCTCGTGAAG GCCAACGAAG GATCCATCGA CCTGAAAGCG 
GCAACCAAAA TCTTGGACGT ACAGAAGCGC CGAATATACG ATATTACCAA TGTTTTAGAG 
GGCATTGGAC TAATTGATAA GGGCAGACAC TGCTCCCTAG TGCGCTGGCG CGGAGGGGGC 
TTTAACAATG CCAAGGACCA AGAGAACTAC GACCTGGCAC GTAGCCGGAC TAATCATTTG 
AAAATGTTGG AGGATGACCT AGACAGGCAA CTGGAGTATG CACAGCGCAA TCTGCGCTAC 
GTTATGCAGG ATCCCTCGAA TAGGTCGTAT GCATATGTGA CACGTGATGA TCTGCTGGAC 
ATCTTTGGAG ATGATTCCGT ATTCACAATA CCTAATTATG ACGAGGAAGT AGATATCAAG 
CGTAATCATT ACGAGCTGGC CGTGTCGCTG GACAATGGCA GCGCAATTGA CATTCGCCTG 
GTGACGAACC AAGGAAAGAG TACTACAAAT CCGCACGATG TGGATGGGTT CTTTGACTAT 
CACCGTCTGG ACACGCCCTC ACCCTCGACG TCGTCGCACT CCAGCGAGGA TGGTAACGCT 
CCAGCATGCG CGGGGAACGT GATCACCGAC GAGCACGGTT ACTCGTGCAA TCCCGGGATG 
AAAGATGAGA TGAAACTTTT GGAGAACGAG CTGACGGCCA AGATAATCTT CCAAAATTAT 
CTGTCCGGTC ATTCGCTGCG GCGATTTTAT CCCGATGATC CGAATCTAGA AAACCCGCCG 
CTGCTGCAGC TGAATCCTCC GCAGGAAGAC TTCAACTTTG CGTTAAAAAG CGACGAAGGT 
ATTTGCGAGC TGTTTGATGT TCAGTGCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477355.1
CDS	190..1302
Translation	MYKRKTASIVKRDSSAAGTTSSAMMMKVDSAETSVRSQSYESTPVSMDTSPDPPTPIKSPSNSQSQSQPGQQRSVGSLVLLTQKFVDLVKANEGSIDLKAATKILDVQKRRIYDITNVLEGIGLIDKGRHCSLVRWRGGGFNNAKDQENYDLARSRTNHLKMLEDDLDRQLEYAQRNLRYVMQDPSNRSYAYVTRDDLLDIFGDDSVFTIPNYDEEVDIKRNHYELAVSLDNGSAIDIRLVTNQGKSTTNPHDVDGFFDYHRLDTPSPSTSSHSSEDGNAPACAGNVITDEHGYSCNPGMKDEMKLLENELTAKIIFQNYLSGHSLRRFYPDDPNLENPPLLQLNPPQEDFNFALKSDEGICELFDVQCS

Target ORF information:

RefSeq Version	NM_058007.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster E2F transcription factor 2 (E2f2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058007.5

ORF Insert Sequence:

ATGTACAAGC GCAAAACCGC GAGTATTGTT AAAAGAGACA GCTCCGCAGC GGGCACCACC 
TCCTCGGCTA TGATGATGAA GGTGGATTCG GCTGAGACTT CGGTCCGGTC GCAGAGCTAC 
GAGTCTACAC CCGTTAGCAT GGACACATCA CCGGATCCTC CAACGCCAAT CAAGTCTCCG 
TCGAATTCAC AATCGCAATC GCAGCCTGGA CAACAGCGCT CCGTGGGCTC ACTGGTCCTG 
CTCACACAGA AGTTTGTGGA TCTCGTGAAG GCCAACGAAG GATCCATCGA CCTGAAAGCG 
GCAACCAAAA TCTTGGACGT ACAGAAGCGC CGAATATACG ATATTACCAA TGTTTTAGAG 
GGCATTGGAC TAATTGATAA GGGCAGACAC TGCTCCCTAG TGCGCTGGCG CGGAGGGGGC 
TTTAACAATG CCAAGGACCA AGAGAACTAC GACCTGGCAC GTAGCCGGAC TAATCATTTG 
AAAATGTTGG AGGATGACCT AGACAGGCAA CTGGAGTATG CACAGCGCAA TCTGCGCTAC 
GTTATGCAGG ATCCCTCGAA TAGGTCGTAT GCATATGTGA CACGTGATGA TCTGCTGGAC 
ATCTTTGGAG ATGATTCCGT ATTCACAATA CCTAATTATG ACGAGGAAGT AGATATCAAG 
CGTAATCATT ACGAGCTGGC CGTGTCGCTG GACAATGGCA GCGCAATTGA CATTCGCCTG 
GTGACGAACC AAGGAAAGAG TACTACAAAT CCGCACGATG TGGATGGGTT CTTTGACTAT 
CACCGTCTGG ACACGCCCTC ACCCTCGACG TCGTCGCACT CCAGCGAGGA TGGTAACGCT 
CCAGCATGCG CGGGGAACGT GATCACCGAC GAGCACGGTT ACTCGTGCAA TCCCGGGATG 
AAAGATGAGA TGAAACTTTT GGAGAACGAG CTGACGGCCA AGATAATCTT CCAAAATTAT 
CTGTCCGGTC ATTCGCTGCG GCGATTTTAT CCCGATGATC CGAATCTAGA AAACCCGCCG 
CTGCTGCAGC TGAATCCTCC GCAGGAAGAC TTCAACTTTG CGTTAAAAAG CGACGAAGGT 
ATTTGCGAGC TGTTTGATGT TCAGTGCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

E2f2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報