Nhe2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Nhe2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nhe2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa16966 NM_001144376.2
Latest version!
Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa16968 NM_001144378.2
Latest version!
Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa16961 NM_001273732.1
Latest version!
Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
OFa16965 NM_001259187.2
Latest version!
Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
OFa15096 NM_136244.5
Latest version!
Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa15096 NM_165355.4
Latest version!
Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa16959 NM_001273733.1
Latest version!
Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa16960 NM_001144377.3
Latest version!
Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa16962 NM_001259188.2
Latest version!
Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa16963 NM_001144379.3
Latest version!
Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa16964 NM_001144375.3
Latest version!
Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa16969 NM_001273731.1
Latest version!
Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa16966
    Clone ID Related Accession (Same CDS sequence) NM_001144376.2
    Accession Version NM_001144376.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Na[+]/H[+] hydrogen exchanger 2, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001144376.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGTATT TTTACATCTA AGTTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137848.1
    CDS536..2554
    Translation

    Target ORF information:

    RefSeq Version NM_001144376.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144376.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGTATT TTTACATCTA AGTTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16968
    Clone ID Related Accession (Same CDS sequence) NM_001144378.2
    Accession Version NM_001144378.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2172bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Na[+]/H[+] hydrogen exchanger 2, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001144378.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGAAAT TAATGAATTG CATTTATTGG ACCACATTCC CATGCAAAGC
    TATGAGCGAG CATTGAACAC GAATAGAGGG CCGAGTGCAT CAAATGCAGA TGGAAAGAGC
    CGAAGGCAAC GTCGTTACTC AAGAATATCT TCTAGAATGA ACCCTTTCAG AAACATGAAT
    GATAAGATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137850.1
    CDS536..2707
    Translation

    Target ORF information:

    RefSeq Version NM_001144378.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144378.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGAAAT TAATGAATTG CATTTATTGG ACCACATTCC CATGCAAAGC
    TATGAGCGAG CATTGAACAC GAATAGAGGG CCGAGTGCAT CAAATGCAGA TGGAAAGAGC
    CGAAGGCAAC GTCGTTACTC AAGAATATCT TCTAGAATGA ACCCTTTCAG AAACATGAAT
    GATAAGATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16961
    Clone ID Related Accession (Same CDS sequence) NM_001273732.1
    Accession Version NM_001273732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Na[+]/H[+] hydrogen exchanger 2, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGGTGGGCA
    CCGCCAACCA TATACACCAC CTGCCCCAGT CTGACAAATC TAGACAATAC CTGTTCCCGT
    AATCTGGACA TGGCTGAGCT GGATTATAAT CCATCCAAGA AGGATCTAAC CGATGCCAGG
    ATCCATCATC TTTTGGCCGA AGAACTGAAG CCTTATAGAA GGCACCGTCG TCTTAGTTAT
    AGCCGACACG CAGTAGATGA CAGAGATTTG TCAACCCAGG TCAATTACAA GATGCAAATG
    AACTTCAGGC GGATGTTTAA TGATCGAAAA CATCACAAAC GCAGCAAACG TGGTGCCAGC
    AACAAGGAGG CCAAGGAGAA CGTTAAGCAG AATCATGTCT CGTTCCATGA CTTTCAACAG
    AACGGCACCA CTAAGCAACT CACCAATGAC TATATTAACA ATGTGCTTAA TGAAACAGCC
    GAGGAGTGCC AACAGAATCC CAACGAGATC AATGTTGTTG GCCCGAGCGA CGATTGGGAT
    GATGGCCTGA CCTTCACCGC CAAATCATCA CTGGCCGAAC ATCCCATTCC CGAGGAGGAT
    CGTAATCTGT CCCGCGAATC CGACGGAGAA AGGCGTGTGG CCACACCCAC CGCCACGGAA
    TCCCAATTGC CGTGGAAAAG ACAGGGTGAC GAATGCACGG ATGCAGTGCA GCAGAACGAG
    TTTCCCGCTT GGGCCTCCAA CAAGGAGTAC TTGGCTTACA ATTCCCCCAG TGCAACATTC
    CTAGGTGGTA TAAACAAGCC TAAACAGCCC AAGTCCGTCA TAGGTCTCTT CCGCCGTGAG
    AGTTCCAGCT CGAAGGCCGG GAGCGTTGGC GTCGGCAGCA CAGGAGCCAT GGACTCCGCC
    GCCAGTGGCT CCGAAACGAT GGTGGTGTCC ATGTCCAGCC AACCGCCCAA CGCTCCATCG
    ACGTCCATCC ACAATCCGCG GCTGGACAAG CGCTCCCAGT CGATCTCCTC AAGCTCGCTG
    GGCGCCGGAG CCCATCAGCT AGGTCCGGAG GGTCATTCCG GCCCATTTCC GGTTACGGCC
    AGTCATCGGC GGAACGTGCG CAGGGGCTCC ATGCTGGAGC TAAGCGGACT CATTGCAACT
    GGACGAAGGC CCAGTAGAAT TTTGCAATTT AGTCCGGGAG CAACTAACTT ACTAGGGTCA
    GCCATGATTC CAAGTCCTTC TCCTCCACCT CCTACTACTT CAACAACAAC AACAACAACA
    ACAACAACTA CAGTAAAACC AACAAGCACA TCAACCACCA AGAACAACAA AACCAACAAT
    TCAACAGATA CAACATCAGC AAGCGCCACA AACTCAACGC CAACCTCCCC GAATTCGGAG
    GACAGCGCCA CATATACGTA CCATACGAAC GGCGTCTCAA TTGAATCTGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260661.1
    CDS530..4063
    Translation

    Target ORF information:

    RefSeq Version NM_001273732.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGGTGGGCA
    CCGCCAACCA TATACACCAC CTGCCCCAGT CTGACAAATC TAGACAATAC CTGTTCCCGT
    AATCTGGACA TGGCTGAGCT GGATTATAAT CCATCCAAGA AGGATCTAAC CGATGCCAGG
    ATCCATCATC TTTTGGCCGA AGAACTGAAG CCTTATAGAA GGCACCGTCG TCTTAGTTAT
    AGCCGACACG CAGTAGATGA CAGAGATTTG TCAACCCAGG TCAATTACAA GATGCAAATG
    AACTTCAGGC GGATGTTTAA TGATCGAAAA CATCACAAAC GCAGCAAACG TGGTGCCAGC
    AACAAGGAGG CCAAGGAGAA CGTTAAGCAG AATCATGTCT CGTTCCATGA CTTTCAACAG
    AACGGCACCA CTAAGCAACT CACCAATGAC TATATTAACA ATGTGCTTAA TGAAACAGCC
    GAGGAGTGCC AACAGAATCC CAACGAGATC AATGTTGTTG GCCCGAGCGA CGATTGGGAT
    GATGGCCTGA CCTTCACCGC CAAATCATCA CTGGCCGAAC ATCCCATTCC CGAGGAGGAT
    CGTAATCTGT CCCGCGAATC CGACGGAGAA AGGCGTGTGG CCACACCCAC CGCCACGGAA
    TCCCAATTGC CGTGGAAAAG ACAGGGTGAC GAATGCACGG ATGCAGTGCA GCAGAACGAG
    TTTCCCGCTT GGGCCTCCAA CAAGGAGTAC TTGGCTTACA ATTCCCCCAG TGCAACATTC
    CTAGGTGGTA TAAACAAGCC TAAACAGCCC AAGTCCGTCA TAGGTCTCTT CCGCCGTGAG
    AGTTCCAGCT CGAAGGCCGG GAGCGTTGGC GTCGGCAGCA CAGGAGCCAT GGACTCCGCC
    GCCAGTGGCT CCGAAACGAT GGTGGTGTCC ATGTCCAGCC AACCGCCCAA CGCTCCATCG
    ACGTCCATCC ACAATCCGCG GCTGGACAAG CGCTCCCAGT CGATCTCCTC AAGCTCGCTG
    GGCGCCGGAG CCCATCAGCT AGGTCCGGAG GGTCATTCCG GCCCATTTCC GGTTACGGCC
    AGTCATCGGC GGAACGTGCG CAGGGGCTCC ATGCTGGAGC TAAGCGGACT CATTGCAACT
    GGACGAAGGC CCAGTAGAAT TTTGCAATTT AGTCCGGGAG CAACTAACTT ACTAGGGTCA
    GCCATGATTC CAAGTCCTTC TCCTCCACCT CCTACTACTT CAACAACAAC AACAACAACA
    ACAACAACTA CAGTAAAACC AACAAGCACA TCAACCACCA AGAACAACAA AACCAACAAT
    TCAACAGATA CAACATCAGC AAGCGCCACA AACTCAACGC CAACCTCCCC GAATTCGGAG
    GACAGCGCCA CATATACGTA CCATACGAAC GGCGTCTCAA TTGAATCTGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16965
    Clone ID Related Accession (Same CDS sequence) NM_001259187.2
    Accession Version NM_001259187.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Na[+]/H[+] hydrogen exchanger 2, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001259187.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGCCCCAGT
    CTGACAAATC TAGACAATAC CTGTTCCCGT AATCTGGACA TGGCTGAGCT GGATTATAAT
    CCATCCAAGA AGGATCTAAC CGATGCCAGG ATCCATCATC TTTTGGCCGA AGAACTGAAG
    CCTTATAGAA GGCACCGTCG TCTTAGTTAT AGCCGACACG CAGTAGATGA CAGAGATTTG
    TCAACCCAGG TCAATTACAA GATGCAAATG AACTTCAGGC GGATGTTTAA TGATCGAAAA
    CATCACAAAC GCAGCAAACG TGGTGCCAGC AACAAGGAGG CCAAGGAGAA CGTTAAGCAG
    AATCATGTCT CGTTCCATGA CTTTCAACAG AACGGCACCA CTAAGCAACT CACCAATGCC
    GAGGAGTGCC AACAGAATCC CAACGAGATC AATGTTGTTG GCCCGAGCGA CGATTGGGAT
    GATGGCCTGA CCTTCACCGC CAAATCATCA CCTGACTCGG ATCGTGCCAA TAACAATTCT
    CTGATAGCCC ACATCCAAAA CCTGCCGGGT TTCGATGCCT CCAAGGCCCG TATCGTCGTC
    CAGCACTATG CGCCCAAGGC CGACGACAAT CCGGACACAG ATATAGAGTC CCCGGAGCAG
    GCCATTGGGC CCACGGCCGC CGAATTGATA TTGCCGTGGC GGCGGGATCG TTCATACCAG
    AGCATTGTGG CCGAACATCC CATTCCCGAG GAGGATCGTA ATCTGTCCCG CGAATCCGAC
    GGAGAAAGGC GTGTGGCCAC ACCCACCGCC ACGGAATCCC AATTGCCGTG GAAAAGACAG
    GGTGACGAAT GCACGGATGC AGTGCAGCAG AACGAGTTTC CCGCTTGGGC CTCCAACAAG
    GAGTACTTGG CTTACAATTC CCCCAGTGCA ACATTCCTAG GTGGTATAAA CAAGCCTAAA
    CAGCCCAAGT CCGTCATAGG TCTCTTCCGC CGTGAGAGTT CCAGCTCGAA GGCCGGGAGC
    GTTGGCGTCG GCAGCACAGG AGCCATGGAC TCCGCCGCCA GTGGCTCCGA AACGATGGTG
    GTGTCCATGT CCAGCCAACC GCCCAACGCT CCATCGACGT CCATCCACAA TCCGCGGCTG
    GACAAGCGCT CCCAGTCGAT CTCCTCAAGC TCGCTGGGCG CCGGAGCCCA TCAGCTAGGT
    CCGGAGGGTC ATTCCGGCCC ATTTCCGGTT ACGGCCAGTC ATCGGCGGAA CGTGCGCAGG
    GGCTCCATGC TGGAGCTAAG CGGACTCATT GCAACTGGAC GAAGGCCCAG TAGAATTTTG
    CAATTTAGTC CGGGAGCAAC TAACTTACTA GGGTCAGCCA TGATTCCAAG TCCTTCTCCT
    CCACCTCCTA CTACTTCAAC AACAACAACA ACAACAACAA CAACTACAGT AAAACCAACA
    AGCACATCAA CCACCAAGAA CAACAAAACC AACAATTCAA CAGATACAAC ATCAGCAAGC
    GCCACAAACT CAACGCCAAC CTCCCCGAAT TCGGAGGACA GCGCCACATA TACGTACCAT
    ACGAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246116.1
    CDS536..4204
    Translation

    Target ORF information:

    RefSeq Version NM_001259187.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259187.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGCCCCAGT
    CTGACAAATC TAGACAATAC CTGTTCCCGT AATCTGGACA TGGCTGAGCT GGATTATAAT
    CCATCCAAGA AGGATCTAAC CGATGCCAGG ATCCATCATC TTTTGGCCGA AGAACTGAAG
    CCTTATAGAA GGCACCGTCG TCTTAGTTAT AGCCGACACG CAGTAGATGA CAGAGATTTG
    TCAACCCAGG TCAATTACAA GATGCAAATG AACTTCAGGC GGATGTTTAA TGATCGAAAA
    CATCACAAAC GCAGCAAACG TGGTGCCAGC AACAAGGAGG CCAAGGAGAA CGTTAAGCAG
    AATCATGTCT CGTTCCATGA CTTTCAACAG AACGGCACCA CTAAGCAACT CACCAATGCC
    GAGGAGTGCC AACAGAATCC CAACGAGATC AATGTTGTTG GCCCGAGCGA CGATTGGGAT
    GATGGCCTGA CCTTCACCGC CAAATCATCA CCTGACTCGG ATCGTGCCAA TAACAATTCT
    CTGATAGCCC ACATCCAAAA CCTGCCGGGT TTCGATGCCT CCAAGGCCCG TATCGTCGTC
    CAGCACTATG CGCCCAAGGC CGACGACAAT CCGGACACAG ATATAGAGTC CCCGGAGCAG
    GCCATTGGGC CCACGGCCGC CGAATTGATA TTGCCGTGGC GGCGGGATCG TTCATACCAG
    AGCATTGTGG CCGAACATCC CATTCCCGAG GAGGATCGTA ATCTGTCCCG CGAATCCGAC
    GGAGAAAGGC GTGTGGCCAC ACCCACCGCC ACGGAATCCC AATTGCCGTG GAAAAGACAG
    GGTGACGAAT GCACGGATGC AGTGCAGCAG AACGAGTTTC CCGCTTGGGC CTCCAACAAG
    GAGTACTTGG CTTACAATTC CCCCAGTGCA ACATTCCTAG GTGGTATAAA CAAGCCTAAA
    CAGCCCAAGT CCGTCATAGG TCTCTTCCGC CGTGAGAGTT CCAGCTCGAA GGCCGGGAGC
    GTTGGCGTCG GCAGCACAGG AGCCATGGAC TCCGCCGCCA GTGGCTCCGA AACGATGGTG
    GTGTCCATGT CCAGCCAACC GCCCAACGCT CCATCGACGT CCATCCACAA TCCGCGGCTG
    GACAAGCGCT CCCAGTCGAT CTCCTCAAGC TCGCTGGGCG CCGGAGCCCA TCAGCTAGGT
    CCGGAGGGTC ATTCCGGCCC ATTTCCGGTT ACGGCCAGTC ATCGGCGGAA CGTGCGCAGG
    GGCTCCATGC TGGAGCTAAG CGGACTCATT GCAACTGGAC GAAGGCCCAG TAGAATTTTG
    CAATTTAGTC CGGGAGCAAC TAACTTACTA GGGTCAGCCA TGATTCCAAG TCCTTCTCCT
    CCACCTCCTA CTACTTCAAC AACAACAACA ACAACAACAA CAACTACAGT AAAACCAACA
    AGCACATCAA CCACCAAGAA CAACAAAACC AACAATTCAA CAGATACAAC ATCAGCAAGC
    GCCACAAACT CAACGCCAAC CTCCCCGAAT TCGGAGGACA GCGCCACATA TACGTACCAT
    ACGAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15096
    Clone ID Related Accession (Same CDS sequence) NM_165355.4 , NM_136244.5
    Accession Version NM_165355.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Na[+]/H[+] hydrogen exchanger 2, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165355.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGCCCCAGT
    CTGACAAATC TAGACAATAC CTGTTCCCGT AATCTGGACA TGGCTGAGCT GGATTATAAT
    CCATCCAAGA AGGATCTAAC CGATGCCAGG ATCCATCATC TTTTGGCCGA AGAACTGAAG
    CCTTATAGAA GGCACCGTCG TCTTAGTTAT AGCCGACACG CAGTAGATGA CAGAGATTTG
    TCAACCCAGG TCAATTACAA GATGCAAATG AACTTCAGGC GGATGTTTAA TGATCGAAAA
    CATCACAAAC GCAGCAAACG TGGTGCCAGC AACAAGGAGG CCAAGGAGAA CGTTAAGCAG
    AATCATGTCT CGTTCCATGA CTTTCAACAG AACGGCACCA CTAAGCAACT CACCAATGCC
    GAGGAGTGCC AACAGAATCC CAACGAGATC AATGTTGTTG GCCCGAGCGA CGATTGGGAT
    GATGGCCTGA CCTTCACCGC CAAATCATCA CCTGACTCGG ATCGTGCCAA TAACAATTCT
    CTGATAGCCC ACATCCAAAA CCTGCCGGGT TTCGATGCCT CCAAGGCCCG TATCGTCGTC
    CAGCACTATG CGCCCAAGGC CGACGACAAT CCGGACACAG ATATAGAGTC CCCGGAGCAG
    GCCATTGGGC CCACGGCCGC CGAATTGATA TTGCCGTGGC GGCGGGATCG TTCATACCAG
    AGCATTGTGG CCGAACATCC CATTCCCGAG GAGGATCGTA ATCTGTCCCG CGAATCCGAC
    GGAGAAAGGC GTGTGGCCAC ACCCACCGCC ACGGAATCCC AATTGCCGTG GAAAAGACAG
    GGTGACGAAT GCACGGATGC AGTGCAGCAG AACGAGTTTC CCGCTTGGGC CTCCAACAAG
    GAGTACTTGG CTTACAATTC CCCCAGTGCA ACATTCCTAG GTGGTATAAA CAAGCCTAAA
    CAGCCCAAGT CCGTCATAGG TCTCTTCCGC CGTGAGAGTT CCAGCTCGAA GGCCGGGAGC
    GTTGGCGTCG GCAGCACAGG AGCCATGGAC TCCGCCGCCA GTGGCTCCGA AACGATGGTG
    GTGTCCATGT CCAGCCAACC GCCCAACGCT CCATCGACGT CCATCCACAA TCCGCGGCTG
    GACAAGCGCT CCCAGTCGAT CTCCTCAAGC TCGCTGGGCG CCGGAGCCCA TCAGCTAGGT
    CCGGAGGGTC ATTCCGGCCC ATTTCCGGTT ACGGCCAGTC ATCGGCGGAA CGTGCGCAGG
    GGCTCCATGC TGGAGCTAAG CGGAGACACA ATACCGGAGG AGTCGTCGTA CCAGCACGGA
    CACTCCAAGT CCTTGTGCGA GCCGGCGGAC TCCGATGAAT GGGAGGGAGC ACCGTTGTCC
    GCAGCCGGCG GAGCAAACAG CGAGCTTTTG ATGCGAATGA GCGGCAGGGA ACCACTCCTG
    CCACGCCCAT CCAACACGCC TCGTGCCCAG ATTCGGCGCA TGAACGCCGG GGCGGTGGGC
    GGTGCAGGCG TGAGCCAGGC GGGCCGTAAG AACCAGGTGA CCAAGGCACT GTTGGACTAC
    GAGGATTCCG AAACCGACTC CGATGAGAAC GATGATGATG AGGACGAGGA CTTCGATTCC
    TACGATGACG AGAACATTGT GGTCACCACC TTTACAACAC CGGCCACGGG CAGAAGACCG
    GGATCTAGTC CAGGATCCGG GTCAGAAGCC AACACCGCCA CTACCACCAC GACAAGCATT
    CGGCTGACCC GCAACAACGA CGAAAGCATC ATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724311.1
    CDS530..4405
    Translation

    Target ORF information:

    RefSeq Version NM_165355.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165355.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGCCCCAGT
    CTGACAAATC TAGACAATAC CTGTTCCCGT AATCTGGACA TGGCTGAGCT GGATTATAAT
    CCATCCAAGA AGGATCTAAC CGATGCCAGG ATCCATCATC TTTTGGCCGA AGAACTGAAG
    CCTTATAGAA GGCACCGTCG TCTTAGTTAT AGCCGACACG CAGTAGATGA CAGAGATTTG
    TCAACCCAGG TCAATTACAA GATGCAAATG AACTTCAGGC GGATGTTTAA TGATCGAAAA
    CATCACAAAC GCAGCAAACG TGGTGCCAGC AACAAGGAGG CCAAGGAGAA CGTTAAGCAG
    AATCATGTCT CGTTCCATGA CTTTCAACAG AACGGCACCA CTAAGCAACT CACCAATGCC
    GAGGAGTGCC AACAGAATCC CAACGAGATC AATGTTGTTG GCCCGAGCGA CGATTGGGAT
    GATGGCCTGA CCTTCACCGC CAAATCATCA CCTGACTCGG ATCGTGCCAA TAACAATTCT
    CTGATAGCCC ACATCCAAAA CCTGCCGGGT TTCGATGCCT CCAAGGCCCG TATCGTCGTC
    CAGCACTATG CGCCCAAGGC CGACGACAAT CCGGACACAG ATATAGAGTC CCCGGAGCAG
    GCCATTGGGC CCACGGCCGC CGAATTGATA TTGCCGTGGC GGCGGGATCG TTCATACCAG
    AGCATTGTGG CCGAACATCC CATTCCCGAG GAGGATCGTA ATCTGTCCCG CGAATCCGAC
    GGAGAAAGGC GTGTGGCCAC ACCCACCGCC ACGGAATCCC AATTGCCGTG GAAAAGACAG
    GGTGACGAAT GCACGGATGC AGTGCAGCAG AACGAGTTTC CCGCTTGGGC CTCCAACAAG
    GAGTACTTGG CTTACAATTC CCCCAGTGCA ACATTCCTAG GTGGTATAAA CAAGCCTAAA
    CAGCCCAAGT CCGTCATAGG TCTCTTCCGC CGTGAGAGTT CCAGCTCGAA GGCCGGGAGC
    GTTGGCGTCG GCAGCACAGG AGCCATGGAC TCCGCCGCCA GTGGCTCCGA AACGATGGTG
    GTGTCCATGT CCAGCCAACC GCCCAACGCT CCATCGACGT CCATCCACAA TCCGCGGCTG
    GACAAGCGCT CCCAGTCGAT CTCCTCAAGC TCGCTGGGCG CCGGAGCCCA TCAGCTAGGT
    CCGGAGGGTC ATTCCGGCCC ATTTCCGGTT ACGGCCAGTC ATCGGCGGAA CGTGCGCAGG
    GGCTCCATGC TGGAGCTAAG CGGAGACACA ATACCGGAGG AGTCGTCGTA CCAGCACGGA
    CACTCCAAGT CCTTGTGCGA GCCGGCGGAC TCCGATGAAT GGGAGGGAGC ACCGTTGTCC
    GCAGCCGGCG GAGCAAACAG CGAGCTTTTG ATGCGAATGA GCGGCAGGGA ACCACTCCTG
    CCACGCCCAT CCAACACGCC TCGTGCCCAG ATTCGGCGCA TGAACGCCGG GGCGGTGGGC
    GGTGCAGGCG TGAGCCAGGC GGGCCGTAAG AACCAGGTGA CCAAGGCACT GTTGGACTAC
    GAGGATTCCG AAACCGACTC CGATGAGAAC GATGATGATG AGGACGAGGA CTTCGATTCC
    TACGATGACG AGAACATTGT GGTCACCACC TTTACAACAC CGGCCACGGG CAGAAGACCG
    GGATCTAGTC CAGGATCCGG GTCAGAAGCC AACACCGCCA CTACCACCAC GACAAGCATT
    CGGCTGACCC GCAACAACGA CGAAAGCATC ATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15096
    Clone ID Related Accession (Same CDS sequence) NM_165355.4 , NM_136244.5
    Accession Version NM_136244.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Na[+]/H[+] hydrogen exchanger 2, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_136244.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGCCCCAGT
    CTGACAAATC TAGACAATAC CTGTTCCCGT AATCTGGACA TGGCTGAGCT GGATTATAAT
    CCATCCAAGA AGGATCTAAC CGATGCCAGG ATCCATCATC TTTTGGCCGA AGAACTGAAG
    CCTTATAGAA GGCACCGTCG TCTTAGTTAT AGCCGACACG CAGTAGATGA CAGAGATTTG
    TCAACCCAGG TCAATTACAA GATGCAAATG AACTTCAGGC GGATGTTTAA TGATCGAAAA
    CATCACAAAC GCAGCAAACG TGGTGCCAGC AACAAGGAGG CCAAGGAGAA CGTTAAGCAG
    AATCATGTCT CGTTCCATGA CTTTCAACAG AACGGCACCA CTAAGCAACT CACCAATGCC
    GAGGAGTGCC AACAGAATCC CAACGAGATC AATGTTGTTG GCCCGAGCGA CGATTGGGAT
    GATGGCCTGA CCTTCACCGC CAAATCATCA CCTGACTCGG ATCGTGCCAA TAACAATTCT
    CTGATAGCCC ACATCCAAAA CCTGCCGGGT TTCGATGCCT CCAAGGCCCG TATCGTCGTC
    CAGCACTATG CGCCCAAGGC CGACGACAAT CCGGACACAG ATATAGAGTC CCCGGAGCAG
    GCCATTGGGC CCACGGCCGC CGAATTGATA TTGCCGTGGC GGCGGGATCG TTCATACCAG
    AGCATTGTGG CCGAACATCC CATTCCCGAG GAGGATCGTA ATCTGTCCCG CGAATCCGAC
    GGAGAAAGGC GTGTGGCCAC ACCCACCGCC ACGGAATCCC AATTGCCGTG GAAAAGACAG
    GGTGACGAAT GCACGGATGC AGTGCAGCAG AACGAGTTTC CCGCTTGGGC CTCCAACAAG
    GAGTACTTGG CTTACAATTC CCCCAGTGCA ACATTCCTAG GTGGTATAAA CAAGCCTAAA
    CAGCCCAAGT CCGTCATAGG TCTCTTCCGC CGTGAGAGTT CCAGCTCGAA GGCCGGGAGC
    GTTGGCGTCG GCAGCACAGG AGCCATGGAC TCCGCCGCCA GTGGCTCCGA AACGATGGTG
    GTGTCCATGT CCAGCCAACC GCCCAACGCT CCATCGACGT CCATCCACAA TCCGCGGCTG
    GACAAGCGCT CCCAGTCGAT CTCCTCAAGC TCGCTGGGCG CCGGAGCCCA TCAGCTAGGT
    CCGGAGGGTC ATTCCGGCCC ATTTCCGGTT ACGGCCAGTC ATCGGCGGAA CGTGCGCAGG
    GGCTCCATGC TGGAGCTAAG CGGAGACACA ATACCGGAGG AGTCGTCGTA CCAGCACGGA
    CACTCCAAGT CCTTGTGCGA GCCGGCGGAC TCCGATGAAT GGGAGGGAGC ACCGTTGTCC
    GCAGCCGGCG GAGCAAACAG CGAGCTTTTG ATGCGAATGA GCGGCAGGGA ACCACTCCTG
    CCACGCCCAT CCAACACGCC TCGTGCCCAG ATTCGGCGCA TGAACGCCGG GGCGGTGGGC
    GGTGCAGGCG TGAGCCAGGC GGGCCGTAAG AACCAGGTGA CCAAGGCACT GTTGGACTAC
    GAGGATTCCG AAACCGACTC CGATGAGAAC GATGATGATG AGGACGAGGA CTTCGATTCC
    TACGATGACG AGAACATTGT GGTCACCACC TTTACAACAC CGGCCACGGG CAGAAGACCG
    GGATCTAGTC CAGGATCCGG GTCAGAAGCC AACACCGCCA CTACCACCAC GACAAGCATT
    CGGCTGACCC GCAACAACGA CGAAAGCATC ATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_610088.2
    CDS536..4411
    Translation

    Target ORF information:

    RefSeq Version NM_136244.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_136244.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGCCCCAGT
    CTGACAAATC TAGACAATAC CTGTTCCCGT AATCTGGACA TGGCTGAGCT GGATTATAAT
    CCATCCAAGA AGGATCTAAC CGATGCCAGG ATCCATCATC TTTTGGCCGA AGAACTGAAG
    CCTTATAGAA GGCACCGTCG TCTTAGTTAT AGCCGACACG CAGTAGATGA CAGAGATTTG
    TCAACCCAGG TCAATTACAA GATGCAAATG AACTTCAGGC GGATGTTTAA TGATCGAAAA
    CATCACAAAC GCAGCAAACG TGGTGCCAGC AACAAGGAGG CCAAGGAGAA CGTTAAGCAG
    AATCATGTCT CGTTCCATGA CTTTCAACAG AACGGCACCA CTAAGCAACT CACCAATGCC
    GAGGAGTGCC AACAGAATCC CAACGAGATC AATGTTGTTG GCCCGAGCGA CGATTGGGAT
    GATGGCCTGA CCTTCACCGC CAAATCATCA CCTGACTCGG ATCGTGCCAA TAACAATTCT
    CTGATAGCCC ACATCCAAAA CCTGCCGGGT TTCGATGCCT CCAAGGCCCG TATCGTCGTC
    CAGCACTATG CGCCCAAGGC CGACGACAAT CCGGACACAG ATATAGAGTC CCCGGAGCAG
    GCCATTGGGC CCACGGCCGC CGAATTGATA TTGCCGTGGC GGCGGGATCG TTCATACCAG
    AGCATTGTGG CCGAACATCC CATTCCCGAG GAGGATCGTA ATCTGTCCCG CGAATCCGAC
    GGAGAAAGGC GTGTGGCCAC ACCCACCGCC ACGGAATCCC AATTGCCGTG GAAAAGACAG
    GGTGACGAAT GCACGGATGC AGTGCAGCAG AACGAGTTTC CCGCTTGGGC CTCCAACAAG
    GAGTACTTGG CTTACAATTC CCCCAGTGCA ACATTCCTAG GTGGTATAAA CAAGCCTAAA
    CAGCCCAAGT CCGTCATAGG TCTCTTCCGC CGTGAGAGTT CCAGCTCGAA GGCCGGGAGC
    GTTGGCGTCG GCAGCACAGG AGCCATGGAC TCCGCCGCCA GTGGCTCCGA AACGATGGTG
    GTGTCCATGT CCAGCCAACC GCCCAACGCT CCATCGACGT CCATCCACAA TCCGCGGCTG
    GACAAGCGCT CCCAGTCGAT CTCCTCAAGC TCGCTGGGCG CCGGAGCCCA TCAGCTAGGT
    CCGGAGGGTC ATTCCGGCCC ATTTCCGGTT ACGGCCAGTC ATCGGCGGAA CGTGCGCAGG
    GGCTCCATGC TGGAGCTAAG CGGAGACACA ATACCGGAGG AGTCGTCGTA CCAGCACGGA
    CACTCCAAGT CCTTGTGCGA GCCGGCGGAC TCCGATGAAT GGGAGGGAGC ACCGTTGTCC
    GCAGCCGGCG GAGCAAACAG CGAGCTTTTG ATGCGAATGA GCGGCAGGGA ACCACTCCTG
    CCACGCCCAT CCAACACGCC TCGTGCCCAG ATTCGGCGCA TGAACGCCGG GGCGGTGGGC
    GGTGCAGGCG TGAGCCAGGC GGGCCGTAAG AACCAGGTGA CCAAGGCACT GTTGGACTAC
    GAGGATTCCG AAACCGACTC CGATGAGAAC GATGATGATG AGGACGAGGA CTTCGATTCC
    TACGATGACG AGAACATTGT GGTCACCACC TTTACAACAC CGGCCACGGG CAGAAGACCG
    GGATCTAGTC CAGGATCCGG GTCAGAAGCC AACACCGCCA CTACCACCAC GACAAGCATT
    CGGCTGACCC GCAACAACGA CGAAAGCATC ATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16959
    Clone ID Related Accession (Same CDS sequence) NM_001273733.1
    Accession Version NM_001273733.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Na[+]/H[+] hydrogen exchanger 2, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGGTGGGCA
    CCGCCAACCA TATACACCAC CTGCCCCAGT CTGACAAATC TAGACAATAC CTGTTCCCGT
    AATCTGGACA TGGCTGAGCT GGATTATAAT CCATCCAAGA AGGATCTAAC CGATGCCAGG
    ATCCATCATC TTTTGGCCGA AGAACTGAAG CCTTATAGAA GGCACCGTCG TCTTAGTTAT
    AGCCGACACG CAGTAGATGA CAGAGATTTG TCAACCCAGG TCAATTACAA GATGCAAATG
    AACTTCAGGC GGATGTTTAA TGATCGAAAA CATCACAAAC GCAGCAAACG TGGTGCCAGC
    AACAAGGAGG CCAAGGAGAA CGTTAAGCAG AATCATGTCT CGTTCCATGA CTTTCAACAG
    AACGGCACCA CTAAGCAACT CACCAATGGT ACGGAATCGA GTTCAAACCA TTCACGAAAA
    AAATCTTACA GAAAAAGACA TACTCACAAT TCACTTAACA AATATCGTAG ATTAGAAATA
    GACTATATTA ACAATGTGCT TAATGAAACA GCCGAGGAGT GCCAACAGAA TCCCAACGAG
    ATCAATGTTG TTGGCCCGAG CGACGATTGG GATGATGGCC TGACCTTCAC CGCCAAATCA
    TCACTGGCCG AACATCCCAT TCCCGAGGAG GATCGTAATC TGTCCCGCGA ATCCGACGGA
    GAAAGGCGTG TGGCCACACC CACCGCCACG GAATCCCAAT TGCCGTGGAA AAGACAGGGT
    GACGAATGCA CGGATGCAGT GCAGCAGAAC GAGTTTCCCG CTTGGGCCTC CAACAAGGAG
    TACTTGGCTT ACAATTCCCC CAGTGCAACA TTCCTAGGTC TCTTCCGCCG TGAGAGTTCC
    AGCTCGAAGG CCGGGAGCGT TGGCGTCGGC AGCACAGGAG CCATGGACTC CGCCGCCAGT
    GGCTCCGAAA CGATGGTGGT GTCCATGTCC AGCCAACCGC CCAACGCTCC ATCGACGTCC
    ATCCACAATC CGCGGCTGGA CAAGCGCTCC CAGTCGATCT CCTCAAGCTC GCTGGGCGCC
    GGAGCCCATC AGCTAGGTCC GGAGGGTCAT TCCGGCCCAT TTCCGGTTAC GGCCAGTCAT
    CGGCGGAACG TGCGCAGGGG CTCCATGCTG GAGCTAAGCG GAGACACAAT ACCGGAGGAG
    TCGTCGTACC AGCACGGACA CTCCAAGTCC TTGTGCGAGC CGGCGGACTC CGATGAATGG
    GAGGGAGCAC CGTTGTCCGC AGCCGGCGGA GCAAACAGCG AGCTTTTGAT GCGAATGAGC
    GGCAGGGAAC CACTCCTGCC ACGCCCATCC AACACGCCTC GTGCCCAGAT TCGGCGCATG
    AACGCCGGGG CGGTGGGCGG TGCAGGCGTG AGCCAGGCGG GCCGTAAGAA CCAGGTGACC
    AAGGCACTGT TGGACTACGA GGATTCCGAA ACCGACTCCG ATGAGAACGA TGATGATGAG
    GACGAGGACT TCGATTCCTA CGATGACGAG AACATTGTGG TCACCACCTT TACAACACCG
    GCCACGGGCA GAAGACCGGG ATCTAGTCCA GGATCCGGGT CAGAAGCCAA CACCGCCACT
    ACCACCACGA CAAGCATTCG GCTGACCCGC AACAACGACG AAAGCATCAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260662.1
    CDS530..4303
    Translation

    Target ORF information:

    RefSeq Version NM_001273733.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGGTGGGCA
    CCGCCAACCA TATACACCAC CTGCCCCAGT CTGACAAATC TAGACAATAC CTGTTCCCGT
    AATCTGGACA TGGCTGAGCT GGATTATAAT CCATCCAAGA AGGATCTAAC CGATGCCAGG
    ATCCATCATC TTTTGGCCGA AGAACTGAAG CCTTATAGAA GGCACCGTCG TCTTAGTTAT
    AGCCGACACG CAGTAGATGA CAGAGATTTG TCAACCCAGG TCAATTACAA GATGCAAATG
    AACTTCAGGC GGATGTTTAA TGATCGAAAA CATCACAAAC GCAGCAAACG TGGTGCCAGC
    AACAAGGAGG CCAAGGAGAA CGTTAAGCAG AATCATGTCT CGTTCCATGA CTTTCAACAG
    AACGGCACCA CTAAGCAACT CACCAATGGT ACGGAATCGA GTTCAAACCA TTCACGAAAA
    AAATCTTACA GAAAAAGACA TACTCACAAT TCACTTAACA AATATCGTAG ATTAGAAATA
    GACTATATTA ACAATGTGCT TAATGAAACA GCCGAGGAGT GCCAACAGAA TCCCAACGAG
    ATCAATGTTG TTGGCCCGAG CGACGATTGG GATGATGGCC TGACCTTCAC CGCCAAATCA
    TCACTGGCCG AACATCCCAT TCCCGAGGAG GATCGTAATC TGTCCCGCGA ATCCGACGGA
    GAAAGGCGTG TGGCCACACC CACCGCCACG GAATCCCAAT TGCCGTGGAA AAGACAGGGT
    GACGAATGCA CGGATGCAGT GCAGCAGAAC GAGTTTCCCG CTTGGGCCTC CAACAAGGAG
    TACTTGGCTT ACAATTCCCC CAGTGCAACA TTCCTAGGTC TCTTCCGCCG TGAGAGTTCC
    AGCTCGAAGG CCGGGAGCGT TGGCGTCGGC AGCACAGGAG CCATGGACTC CGCCGCCAGT
    GGCTCCGAAA CGATGGTGGT GTCCATGTCC AGCCAACCGC CCAACGCTCC ATCGACGTCC
    ATCCACAATC CGCGGCTGGA CAAGCGCTCC CAGTCGATCT CCTCAAGCTC GCTGGGCGCC
    GGAGCCCATC AGCTAGGTCC GGAGGGTCAT TCCGGCCCAT TTCCGGTTAC GGCCAGTCAT
    CGGCGGAACG TGCGCAGGGG CTCCATGCTG GAGCTAAGCG GAGACACAAT ACCGGAGGAG
    TCGTCGTACC AGCACGGACA CTCCAAGTCC TTGTGCGAGC CGGCGGACTC CGATGAATGG
    GAGGGAGCAC CGTTGTCCGC AGCCGGCGGA GCAAACAGCG AGCTTTTGAT GCGAATGAGC
    GGCAGGGAAC CACTCCTGCC ACGCCCATCC AACACGCCTC GTGCCCAGAT TCGGCGCATG
    AACGCCGGGG CGGTGGGCGG TGCAGGCGTG AGCCAGGCGG GCCGTAAGAA CCAGGTGACC
    AAGGCACTGT TGGACTACGA GGATTCCGAA ACCGACTCCG ATGAGAACGA TGATGATGAG
    GACGAGGACT TCGATTCCTA CGATGACGAG AACATTGTGG TCACCACCTT TACAACACCG
    GCCACGGGCA GAAGACCGGG ATCTAGTCCA GGATCCGGGT CAGAAGCCAA CACCGCCACT
    ACCACCACGA CAAGCATTCG GCTGACCCGC AACAACGACG AAAGCATCAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16960
    Clone ID Related Accession (Same CDS sequence) NM_001144377.3
    Accession Version NM_001144377.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Na[+]/H[+] hydrogen exchanger 2, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001144377.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGGTGGGCA
    CCGCCAACCA TATACACCAC CTGCCCCAGT CTGACAAATC TAGACAATAC CTGTTCCCGT
    AATCTGGACA TGGCTGAGCT GGATTATAAT CCATCCAAGA AGGATCTAAC CGATGCCAGG
    ATCCATCATC TTTTGGCCGA AGAACTGAAG CCTTATAGAA GGCACCGTCG TCTTAGTTAT
    AGCCGACACG CAGTAGATGA CAGAGATTTG TCAACCCAGG TCAATTACAA GATGCAAATG
    AACTTCAGGC GGATGTTTAA TGATCGAAAA CATCACAAAC GCAGCAAACG TGGTGCCAGC
    AACAAGGAGG CCAAGGAGAA CGTTAAGCAG AATCATGTCT CGTTCCATGA CTTTCAACAG
    AACGGCACCA CTAAGCAACT CACCAATGCC GAGGAGTGCC AACAGAATCC CAACGAGATC
    AATGTTGTTG GCCCGAGCGA CGATTGGGAT GATGGCCTGA CCTTCACCGC CAAATCATCA
    CTGGCCGAAC ATCCCATTCC CGAGGAGGAT CGTAATCTGT CCCGCGAATC CGACGGAGAA
    AGGCGTGTGG CCACACCCAC CGCCACGGAA TCCCAATTGC CGTGGAAAAG ACAGGGTGAC
    GAATGCACGG ATGCAGTGCA GCAGAACGAG TTTCCCGCTT GGGCCTCCAA CAAGGAGTAC
    TTGGCTTACA ATTCCCCCAG TGCAACATTC CTAGGTGGTA TAAACAAGCC TAAACAGCCC
    AAGTCCGTCA TAGGTCTCTT CCGCCGTGAG AGTTCCAGCT CGAAGGCCGG GAGCGTTGGC
    GTCGGCAGCA CAGGAGCCAT GGACTCCGCC GCCAGTGGCT CCGAAACGAT GGTGGTGTCC
    ATGTCCAGCC AACCGCCCAA CGCTCCATCG ACGTCCATCC ACAATCCGCG GCTGGACAAG
    CGCTCCCAGT CGATCTCCTC AAGCTCGCTG GGCGCCGGAG CCCATCAGCT AGGTCCGGAG
    GGTCATTCCG GCCCATTTCC GGTTACGGCC AGTCATCGGC GGAACGTGCG CAGGGGCTCC
    ATGCTGGAGC TAAGCGGAGA CACAATACCG GAGGAGTCGT CGTACCAGCA CGGACACTCC
    AAGTCCTTGT GCGAGCCGGC GGACTCCGAT GAATGGGAGG GAGCACCGTT GTCCGCAGCC
    GGCGGAGCAA ACAGCGAGCT TTTGATGCGA ATGAGCGGCA GGGAACCACT CCTGCCACGC
    CCATCCAACA CGCCTCGTGC CCAGATTCGG CGCATGAACG CCGGGGCGGT GGGCGGTGCA
    GGCGTGAGCC AGGCGGGCCG TAAGAACCAG GTGACCAAGG CACTGTTGGA CTACGAGGAT
    TCCGAAACCG ACTCCGATGA GAACGATGAT GATGAGGACG AGGACTTCGA TTCCTACGAT
    GACGAGAACA TTGTGGTCAC CACCTTTACA ACACCGGCCA CGGGCAGAAG ACCGGGATCT
    AGTCCAGGAT CCGGGTCAGA AGCCAACACC GCCACTACCA CCACGACAAG CATTCGGCTG
    ACCCGCAACA ACGACGAAAG CATCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137849.1
    CDS530..4219
    Translation

    Target ORF information:

    RefSeq Version NM_001144377.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144377.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGGTGGGCA
    CCGCCAACCA TATACACCAC CTGCCCCAGT CTGACAAATC TAGACAATAC CTGTTCCCGT
    AATCTGGACA TGGCTGAGCT GGATTATAAT CCATCCAAGA AGGATCTAAC CGATGCCAGG
    ATCCATCATC TTTTGGCCGA AGAACTGAAG CCTTATAGAA GGCACCGTCG TCTTAGTTAT
    AGCCGACACG CAGTAGATGA CAGAGATTTG TCAACCCAGG TCAATTACAA GATGCAAATG
    AACTTCAGGC GGATGTTTAA TGATCGAAAA CATCACAAAC GCAGCAAACG TGGTGCCAGC
    AACAAGGAGG CCAAGGAGAA CGTTAAGCAG AATCATGTCT CGTTCCATGA CTTTCAACAG
    AACGGCACCA CTAAGCAACT CACCAATGCC GAGGAGTGCC AACAGAATCC CAACGAGATC
    AATGTTGTTG GCCCGAGCGA CGATTGGGAT GATGGCCTGA CCTTCACCGC CAAATCATCA
    CTGGCCGAAC ATCCCATTCC CGAGGAGGAT CGTAATCTGT CCCGCGAATC CGACGGAGAA
    AGGCGTGTGG CCACACCCAC CGCCACGGAA TCCCAATTGC CGTGGAAAAG ACAGGGTGAC
    GAATGCACGG ATGCAGTGCA GCAGAACGAG TTTCCCGCTT GGGCCTCCAA CAAGGAGTAC
    TTGGCTTACA ATTCCCCCAG TGCAACATTC CTAGGTGGTA TAAACAAGCC TAAACAGCCC
    AAGTCCGTCA TAGGTCTCTT CCGCCGTGAG AGTTCCAGCT CGAAGGCCGG GAGCGTTGGC
    GTCGGCAGCA CAGGAGCCAT GGACTCCGCC GCCAGTGGCT CCGAAACGAT GGTGGTGTCC
    ATGTCCAGCC AACCGCCCAA CGCTCCATCG ACGTCCATCC ACAATCCGCG GCTGGACAAG
    CGCTCCCAGT CGATCTCCTC AAGCTCGCTG GGCGCCGGAG CCCATCAGCT AGGTCCGGAG
    GGTCATTCCG GCCCATTTCC GGTTACGGCC AGTCATCGGC GGAACGTGCG CAGGGGCTCC
    ATGCTGGAGC TAAGCGGAGA CACAATACCG GAGGAGTCGT CGTACCAGCA CGGACACTCC
    AAGTCCTTGT GCGAGCCGGC GGACTCCGAT GAATGGGAGG GAGCACCGTT GTCCGCAGCC
    GGCGGAGCAA ACAGCGAGCT TTTGATGCGA ATGAGCGGCA GGGAACCACT CCTGCCACGC
    CCATCCAACA CGCCTCGTGC CCAGATTCGG CGCATGAACG CCGGGGCGGT GGGCGGTGCA
    GGCGTGAGCC AGGCGGGCCG TAAGAACCAG GTGACCAAGG CACTGTTGGA CTACGAGGAT
    TCCGAAACCG ACTCCGATGA GAACGATGAT GATGAGGACG AGGACTTCGA TTCCTACGAT
    GACGAGAACA TTGTGGTCAC CACCTTTACA ACACCGGCCA CGGGCAGAAG ACCGGGATCT
    AGTCCAGGAT CCGGGTCAGA AGCCAACACC GCCACTACCA CCACGACAAG CATTCGGCTG
    ACCCGCAACA ACGACGAAAG CATCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16962
    Clone ID Related Accession (Same CDS sequence) NM_001259188.2
    Accession Version NM_001259188.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Na[+]/H[+] hydrogen exchanger 2, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001259188.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGCCCCAGT
    CTGACAAATC TAGACAATAC CTGTTCCCGT AATCTGGACA TGGCTGAGCT GGATTATAAT
    CCATCCAAGA AGGATCTAAC CGATGCCAGG ATCCATCATC TTTTGGCCGA AGAACTGAAG
    CCTTATAGAA GGCACCGTCG TCTTAGTTAT AGCCGACACG CAGTAGATGA CAGAGATTTG
    TCAACCCAGG TCAATTACAA GATGCAAATG AACTTCAGGC GGATGTTTAA TGATCGAAAA
    CATCACAAAC GCAGCAAACG TGGTGCCAGC AACAAGGAGG CCAAGGAGAA CGTTAAGCAG
    AATCATGTCT CGTTCCATGA CTTTCAACAG AACGGCACCA CTAAGCAACT CACCAATGGT
    ACGGAATCGA GTTCAAACCA TTCACGAAAA AAATCTTACA GAAAAAGACA TACTCACAAT
    TCACTTAACA AATATCGTAG ATTAGAAATA GCCGAGGAGT GCCAACAGAA TCCCAACGAG
    ATCAATGTTG TTGGCCCGAG CGACGATTGG GATGATGGCC TGACCTTCAC CGCCAAATCA
    TCACCTGACT CGGATCGTGC CAATAACAAT TCTCTGATAG CCCACATCCA AAACCTGCCG
    GGTTTCGATG CCTCCAAGGC CCGTATCGTC GTCCAGCACT ATGCGCCCAA GGCCGACGAC
    AATCCGGACA CAGATATAGA GTCCCCGGAG CAGGCCATTG GGCCCACGGC CGCCGAATTG
    ATATTGCCGT GGCGGCGGGA TCGTTCATAC CAGAGCATTG TGGCCGAACA TCCCATTCCC
    GAGGAGGATC GTAATCTGTC CCGCGAATCC GACGGAGAAA GGCGTGTGGC CACACCCACC
    GCCACGGAAT CCCAATTGCC GTGGAAAAGA CAGGGTGACG AATGCACGGA TGCAGTGCAG
    CAGAACGAGT TTCCCGCTTG GGCCTCCAAC AAGGAGTACT TGGCTTACAA TTCCCCCAGT
    GCAACATTCC TAGGTGGTAT AAACAAGCCT AAACAGCCCA AGTCCGTCAT AGGTCTCTTC
    CGCCGTGAGA GTTCCAGCTC GAAGGCCGGG AGCGTTGGCG TCGGCAGCAC AGGAGCCATG
    GACTCCGCCG CCAGTGGCTC CGAAACGATG GTGGTGTCCA TGTCCAGCCA ACCGCCCAAC
    GCTCCATCGA CGTCCATCCA CAATCCGCGG CTGGACAAGC GCTCCCAGTC GATCTCCTCA
    AGCTCGCTGG GCGCCGGAGC CCATCAGCTA GGTCCGGAGG GTCATTCCGG CCCATTTCCG
    GTTACGGCCA GTCATCGGCG GAACGTGCGC AGGGGCTCCA TGCTGGAGCT AAGCGGCGGC
    GTCTCAATTG AATCTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246117.1
    CDS536..4036
    Translation

    Target ORF information:

    RefSeq Version NM_001259188.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259188.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGCCCCAGT
    CTGACAAATC TAGACAATAC CTGTTCCCGT AATCTGGACA TGGCTGAGCT GGATTATAAT
    CCATCCAAGA AGGATCTAAC CGATGCCAGG ATCCATCATC TTTTGGCCGA AGAACTGAAG
    CCTTATAGAA GGCACCGTCG TCTTAGTTAT AGCCGACACG CAGTAGATGA CAGAGATTTG
    TCAACCCAGG TCAATTACAA GATGCAAATG AACTTCAGGC GGATGTTTAA TGATCGAAAA
    CATCACAAAC GCAGCAAACG TGGTGCCAGC AACAAGGAGG CCAAGGAGAA CGTTAAGCAG
    AATCATGTCT CGTTCCATGA CTTTCAACAG AACGGCACCA CTAAGCAACT CACCAATGGT
    ACGGAATCGA GTTCAAACCA TTCACGAAAA AAATCTTACA GAAAAAGACA TACTCACAAT
    TCACTTAACA AATATCGTAG ATTAGAAATA GCCGAGGAGT GCCAACAGAA TCCCAACGAG
    ATCAATGTTG TTGGCCCGAG CGACGATTGG GATGATGGCC TGACCTTCAC CGCCAAATCA
    TCACCTGACT CGGATCGTGC CAATAACAAT TCTCTGATAG CCCACATCCA AAACCTGCCG
    GGTTTCGATG CCTCCAAGGC CCGTATCGTC GTCCAGCACT ATGCGCCCAA GGCCGACGAC
    AATCCGGACA CAGATATAGA GTCCCCGGAG CAGGCCATTG GGCCCACGGC CGCCGAATTG
    ATATTGCCGT GGCGGCGGGA TCGTTCATAC CAGAGCATTG TGGCCGAACA TCCCATTCCC
    GAGGAGGATC GTAATCTGTC CCGCGAATCC GACGGAGAAA GGCGTGTGGC CACACCCACC
    GCCACGGAAT CCCAATTGCC GTGGAAAAGA CAGGGTGACG AATGCACGGA TGCAGTGCAG
    CAGAACGAGT TTCCCGCTTG GGCCTCCAAC AAGGAGTACT TGGCTTACAA TTCCCCCAGT
    GCAACATTCC TAGGTGGTAT AAACAAGCCT AAACAGCCCA AGTCCGTCAT AGGTCTCTTC
    CGCCGTGAGA GTTCCAGCTC GAAGGCCGGG AGCGTTGGCG TCGGCAGCAC AGGAGCCATG
    GACTCCGCCG CCAGTGGCTC CGAAACGATG GTGGTGTCCA TGTCCAGCCA ACCGCCCAAC
    GCTCCATCGA CGTCCATCCA CAATCCGCGG CTGGACAAGC GCTCCCAGTC GATCTCCTCA
    AGCTCGCTGG GCGCCGGAGC CCATCAGCTA GGTCCGGAGG GTCATTCCGG CCCATTTCCG
    GTTACGGCCA GTCATCGGCG GAACGTGCGC AGGGGCTCCA TGCTGGAGCT AAGCGGCGGC
    GTCTCAATTG AATCTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16963
    Clone ID Related Accession (Same CDS sequence) NM_001144379.3
    Accession Version NM_001144379.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Na[+]/H[+] hydrogen exchanger 2, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001144379.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGCCCCAGT
    CTGACAAATC TAGACAATAC CTGTTCCCGT AATCTGGACA TGGCTGAGCT GGATTATAAT
    CCATCCAAGA AGGATCTAAC CGATGCCAGG ATCCATCATC TTTTGGCCGA AGAACTGAAG
    CCTTATAGAA GGCACCGTCG TCTTAGTTAT AGCCGACACG CAGTAGATGA CAGAGATTTG
    TCAACCCAGG TCAATTACAA GATGCAAATG AACTTCAGGC GGATGTTTAA TGATCGAAAA
    CATCACAAAC GCAGCAAACG TGGTGCCAGC AACAAGGAGG CCAAGGAGAA CGTTAAGCAG
    AATCATGTCT CGTTCCATGA CTTTCAACAG AACGGCACCA CTAAGCAACT CACCAATGGT
    ACGGAATCGA GTTCAAACCA TTCACGAAAA AAATCTTACA GAAAAAGACA TACTCACAAT
    TCACTTAACA AATATCGTAG ATTAGAAATA GCCGAGGAGT GCCAACAGAA TCCCAACGAG
    ATCAATGTTG TTGGCCCGAG CGACGATTGG GATGATGGCC TGACCTTCAC CGCCAAATCA
    TCACCTGACT CGGATCGTGC CAATAACAAT TCTCTGATAG CCCACATCCA AAACCTGCCG
    GGTTTCGATG CCTCCAAGGC CCGTATCGTC GTCCAGCACT ATGCGCCCAA GGCCGACGAC
    AATCCGGACA CAGATATAGA GTCCCCGGAG CAGGCCATTG GGCCCACGGC CGCCGAATTG
    ATATTGCCGT GGCGGCGGGA TCGTTCATAC CAGAGCATTG TGGCCGAACA TCCCATTCCC
    GAGGAGGATC GTAATCTGTC CCGCGAATCC GACGGAGAAA GGCGTGTGGC CACACCCACC
    GCCACGGAAT CCCAATTGCC GTGGAAAAGA CAGGGTGACG AATGCACGGA TGCAGTGCAG
    CAGAACGAGT TTCCCGCTTG GGCCTCCAAC AAGGAGTACT TGGCTTACAA TTCCCCCAGT
    GCAACATTCC TAGGTGGTAT AAACAAGCCT AAACAGCCCA AGTCCGTCAT AGGTCTCTTC
    CGCCGTGAGA GTTCCAGCTC GAAGGCCGGG AGCGTTGGCG TCGGCAGCAC AGGAGCCATG
    GACTCCGCCG CCAGTGGCTC CGAAACGATG GTGGTGTCCA TGTCCAGCCA ACCGCCCAAC
    GCTCCATCGA CGTCCATCCA CAATCCGCGG CTGGACAAGC GCTCCCAGTC GATCTCCTCA
    AGCTCGCTGG GCGCCGGAGC CCATCAGCTA GGTCCGGAGG GTCATTCCGG CCCATTTCCG
    GTTACGGCCA GTCATCGGCG GAACGTGCGC AGGGGCTCCA TGCTGGAGCT AAGCGGAGAC
    ACAATACCGG AGGAGTCGTC GTACCAGCAC GGACACTCCA AGTCCTTGTG CGAGCCGGCG
    GACTCCGATG AATGGGAGGG AGCACCGTTG TCCGCAGCCG GCGGAGCAAA CAGCGAGCTT
    TTGATGCGAA TGAGCGGCAG GGAACCACTC CTGCCACGCC CATCCAACAC GCCTCGTGCC
    CAGATTCGGC GCATGAACGC CGGGGCGGTG GGCGGTGCAG GCGTGAGCCA GGCGGGCCGT
    AAGAACCAGG TGACCAAGGC ACTGTTGGAC TACGAGGATT CCGAAACCGA CTCCGATGAG
    AACGATGATG ATGAGGACGA GGACTTCGAT TCCTACGATG ACGAGAACAT TGTGGTCACC
    ACCTTTACAA CACCGGCCAC GGGCAGAAGA CCGGGATCTA GTCCAGGATC CGGGTCAGAA
    GCCAACACCG CCACTACCAC CACGACAAGC ATTCGGCTGA CCCGCAACAA CGACGAAAGC
    ATCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137851.1
    CDS536..4504
    Translation

    Target ORF information:

    RefSeq Version NM_001144379.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144379.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGCCCCAGT
    CTGACAAATC TAGACAATAC CTGTTCCCGT AATCTGGACA TGGCTGAGCT GGATTATAAT
    CCATCCAAGA AGGATCTAAC CGATGCCAGG ATCCATCATC TTTTGGCCGA AGAACTGAAG
    CCTTATAGAA GGCACCGTCG TCTTAGTTAT AGCCGACACG CAGTAGATGA CAGAGATTTG
    TCAACCCAGG TCAATTACAA GATGCAAATG AACTTCAGGC GGATGTTTAA TGATCGAAAA
    CATCACAAAC GCAGCAAACG TGGTGCCAGC AACAAGGAGG CCAAGGAGAA CGTTAAGCAG
    AATCATGTCT CGTTCCATGA CTTTCAACAG AACGGCACCA CTAAGCAACT CACCAATGGT
    ACGGAATCGA GTTCAAACCA TTCACGAAAA AAATCTTACA GAAAAAGACA TACTCACAAT
    TCACTTAACA AATATCGTAG ATTAGAAATA GCCGAGGAGT GCCAACAGAA TCCCAACGAG
    ATCAATGTTG TTGGCCCGAG CGACGATTGG GATGATGGCC TGACCTTCAC CGCCAAATCA
    TCACCTGACT CGGATCGTGC CAATAACAAT TCTCTGATAG CCCACATCCA AAACCTGCCG
    GGTTTCGATG CCTCCAAGGC CCGTATCGTC GTCCAGCACT ATGCGCCCAA GGCCGACGAC
    AATCCGGACA CAGATATAGA GTCCCCGGAG CAGGCCATTG GGCCCACGGC CGCCGAATTG
    ATATTGCCGT GGCGGCGGGA TCGTTCATAC CAGAGCATTG TGGCCGAACA TCCCATTCCC
    GAGGAGGATC GTAATCTGTC CCGCGAATCC GACGGAGAAA GGCGTGTGGC CACACCCACC
    GCCACGGAAT CCCAATTGCC GTGGAAAAGA CAGGGTGACG AATGCACGGA TGCAGTGCAG
    CAGAACGAGT TTCCCGCTTG GGCCTCCAAC AAGGAGTACT TGGCTTACAA TTCCCCCAGT
    GCAACATTCC TAGGTGGTAT AAACAAGCCT AAACAGCCCA AGTCCGTCAT AGGTCTCTTC
    CGCCGTGAGA GTTCCAGCTC GAAGGCCGGG AGCGTTGGCG TCGGCAGCAC AGGAGCCATG
    GACTCCGCCG CCAGTGGCTC CGAAACGATG GTGGTGTCCA TGTCCAGCCA ACCGCCCAAC
    GCTCCATCGA CGTCCATCCA CAATCCGCGG CTGGACAAGC GCTCCCAGTC GATCTCCTCA
    AGCTCGCTGG GCGCCGGAGC CCATCAGCTA GGTCCGGAGG GTCATTCCGG CCCATTTCCG
    GTTACGGCCA GTCATCGGCG GAACGTGCGC AGGGGCTCCA TGCTGGAGCT AAGCGGAGAC
    ACAATACCGG AGGAGTCGTC GTACCAGCAC GGACACTCCA AGTCCTTGTG CGAGCCGGCG
    GACTCCGATG AATGGGAGGG AGCACCGTTG TCCGCAGCCG GCGGAGCAAA CAGCGAGCTT
    TTGATGCGAA TGAGCGGCAG GGAACCACTC CTGCCACGCC CATCCAACAC GCCTCGTGCC
    CAGATTCGGC GCATGAACGC CGGGGCGGTG GGCGGTGCAG GCGTGAGCCA GGCGGGCCGT
    AAGAACCAGG TGACCAAGGC ACTGTTGGAC TACGAGGATT CCGAAACCGA CTCCGATGAG
    AACGATGATG ATGAGGACGA GGACTTCGAT TCCTACGATG ACGAGAACAT TGTGGTCACC
    ACCTTTACAA CACCGGCCAC GGGCAGAAGA CCGGGATCTA GTCCAGGATC CGGGTCAGAA
    GCCAACACCG CCACTACCAC CACGACAAGC ATTCGGCTGA CCCGCAACAA CGACGAAAGC
    ATCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16964
    Clone ID Related Accession (Same CDS sequence) NM_001144375.3
    Accession Version NM_001144375.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Na[+]/H[+] hydrogen exchanger 2, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001144375.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGCCCCAGT
    CTGACAAATC TAGACAATAC CTGTTCCCGT AATCTGGACA TGGCTGAGCT GGATTATAAT
    CCATCCAAGA AGGATCTAAC CGATGCCAGG ATCCATCATC TTTTGGCCGA AGAACTGAAG
    CCTTATAGAA GGCACCGTCG TCTTAGTTAT AGCCGACACG CAGTAGATGA CAGAGATTTG
    TCAACCCAGG TCAATTACAA GATGCAAATG AACTTCAGGC GGATGTTTAA TGATCGAAAA
    CATCACAAAC GCAGCAAACG TGGTGCCAGC AACAAGGAGG CCAAGGAGAA CGTTAAGCAG
    AATCATGTCT CGTTCCATGA CTTTCAACAG AACGGCACCA CTAAGCAACT CACCAATGCC
    GAGGAGTGCC AACAGAATCC CAACGAGATC AATGTTGTTG GCCCGAGCGA CGATTGGGAT
    GATGGCCTGA CCTTCACCGC CAAATCATCA CTGGCCGAAC ATCCCATTCC CGAGGAGGAT
    CGTAATCTGT CCCGCGAATC CGACGGAGAA AGGCGTGTGG CCACACCCAC CGCCACGGAA
    TCCCAATTGC CGTGGAAAAG ACAGGGTGAC GAATGCACGG ATGCAGTGCA GCAGAACGAG
    TTTCCCGCTT GGGCCTCCAA CAAGGAGTAC TTGGCTTACA ATTCCCCCAG TGCAACATTC
    CTAGGTGGTA TAAACAAGCC TAAACAGCCC AAGTCCGTCA TAGGTCTCTT CCGCCGTGAG
    AGTTCCAGCT CGAAGGCCGG GAGCGTTGGC GTCGGCAGCA CAGGAGCCAT GGACTCCGCC
    GCCAGTGGCT CCGAAACGAT GGTGGTGTCC ATGTCCAGCC AACCGCCCAA CGCTCCATCG
    ACGTCCATCC ACAATCCGCG GCTGGACAAG CGCTCCCAGT CGATCTCCTC AAGCTCGCTG
    GGCGCCGGAG CCCATCAGCT AGGTCCGGAG GGTCATTCCG GCCCATTTCC GGTTACGGCC
    AGTCATCGGC GGAACGTGCG CAGGGGCTCC ATGCTGGAGC TAAGCGGAGA CACAATACCG
    GAGGAGTCGT CGTACCAGCA CGGACACTCC AAGTCCTTGT GCGAGCCGGC GGACTCCGAT
    GAATGGGAGG GAGCACCGTT GTCCGCAGCC GGCGGAGCAA ACAGCGAGCT TTTGATGCGA
    ATGAGCGGCA GGGAACCACT CCTGCCACGC CCATCCAACA CGCCTCGTGC CCAGATTCGG
    CGCATGAACG CCGGGGCGGT GGGCGGTGCA GGCGTGAGCC AGGCGGGCCG TAAGAACCAG
    GTGACCAAGG CACTGTTGGA CTACGAGGAT TCCGAAACCG ACTCCGATGA GAACGATGAT
    GATGAGGACG AGGACTTCGA TTCCTACGAT GACGAGAACA TTGTGGTCAC CACCTTTACA
    ACACCGGCCA CGGGCAGAAG ACCGGGATCT AGTCCAGGAT CCGGGTCAGA AGCCAACACC
    GCCACTACCA CCACGACAAG CATTCGGCTG ACCCGCAACA ACGACGAAAG CATCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137847.1
    CDS536..4195
    Translation

    Target ORF information:

    RefSeq Version NM_001144375.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144375.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGCCCCAGT
    CTGACAAATC TAGACAATAC CTGTTCCCGT AATCTGGACA TGGCTGAGCT GGATTATAAT
    CCATCCAAGA AGGATCTAAC CGATGCCAGG ATCCATCATC TTTTGGCCGA AGAACTGAAG
    CCTTATAGAA GGCACCGTCG TCTTAGTTAT AGCCGACACG CAGTAGATGA CAGAGATTTG
    TCAACCCAGG TCAATTACAA GATGCAAATG AACTTCAGGC GGATGTTTAA TGATCGAAAA
    CATCACAAAC GCAGCAAACG TGGTGCCAGC AACAAGGAGG CCAAGGAGAA CGTTAAGCAG
    AATCATGTCT CGTTCCATGA CTTTCAACAG AACGGCACCA CTAAGCAACT CACCAATGCC
    GAGGAGTGCC AACAGAATCC CAACGAGATC AATGTTGTTG GCCCGAGCGA CGATTGGGAT
    GATGGCCTGA CCTTCACCGC CAAATCATCA CTGGCCGAAC ATCCCATTCC CGAGGAGGAT
    CGTAATCTGT CCCGCGAATC CGACGGAGAA AGGCGTGTGG CCACACCCAC CGCCACGGAA
    TCCCAATTGC CGTGGAAAAG ACAGGGTGAC GAATGCACGG ATGCAGTGCA GCAGAACGAG
    TTTCCCGCTT GGGCCTCCAA CAAGGAGTAC TTGGCTTACA ATTCCCCCAG TGCAACATTC
    CTAGGTGGTA TAAACAAGCC TAAACAGCCC AAGTCCGTCA TAGGTCTCTT CCGCCGTGAG
    AGTTCCAGCT CGAAGGCCGG GAGCGTTGGC GTCGGCAGCA CAGGAGCCAT GGACTCCGCC
    GCCAGTGGCT CCGAAACGAT GGTGGTGTCC ATGTCCAGCC AACCGCCCAA CGCTCCATCG
    ACGTCCATCC ACAATCCGCG GCTGGACAAG CGCTCCCAGT CGATCTCCTC AAGCTCGCTG
    GGCGCCGGAG CCCATCAGCT AGGTCCGGAG GGTCATTCCG GCCCATTTCC GGTTACGGCC
    AGTCATCGGC GGAACGTGCG CAGGGGCTCC ATGCTGGAGC TAAGCGGAGA CACAATACCG
    GAGGAGTCGT CGTACCAGCA CGGACACTCC AAGTCCTTGT GCGAGCCGGC GGACTCCGAT
    GAATGGGAGG GAGCACCGTT GTCCGCAGCC GGCGGAGCAA ACAGCGAGCT TTTGATGCGA
    ATGAGCGGCA GGGAACCACT CCTGCCACGC CCATCCAACA CGCCTCGTGC CCAGATTCGG
    CGCATGAACG CCGGGGCGGT GGGCGGTGCA GGCGTGAGCC AGGCGGGCCG TAAGAACCAG
    GTGACCAAGG CACTGTTGGA CTACGAGGAT TCCGAAACCG ACTCCGATGA GAACGATGAT
    GATGAGGACG AGGACTTCGA TTCCTACGAT GACGAGAACA TTGTGGTCAC CACCTTTACA
    ACACCGGCCA CGGGCAGAAG ACCGGGATCT AGTCCAGGAT CCGGGTCAGA AGCCAACACC
    GCCACTACCA CCACGACAAG CATTCGGCTG ACCCGCAACA ACGACGAAAG CATCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16969
    Clone ID Related Accession (Same CDS sequence) NM_001273731.1
    Accession Version NM_001273731.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Na[+]/H[+] hydrogen exchanger 2, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGGTGGGCA
    CCGCCAACCA TATACACCAC CTGCCCCAGT CTGACAAATC TAGACAATAC CTGTTCCCGT
    AATCTGGACA TGGCTGAGCT GGATTATAAT CCATCCAAGA AGGATCTAAC CGATGCCAGG
    ATCCATCATC TTTTGGCCGA AGAACTGAAG CCTTATAGAA GGGCCTCAAT ACAAATGCAC
    CGTCGTCTTA GTTATAGCCG ACACGCAGTA GATGACAGAG ATTTGTCAAC CCAGGTCAAT
    TACAAGATGC AAATGAACTT CAGGCGGATG TTTAATGATC GAAAACATCA CAAACGCAGC
    AAACGTGGTG CCAGCAACAA GGCCAAGGAG AACGTTAAGC AGAATCATGT CTCGTTCCAT
    GACTTTCAAC AGAACGGCAC CACTAAGCAA CTCACCAATG CCGAGGAGTG CCAACAGAAT
    CCCAACGAGA TCAATGTTGT TGGCCCGAGC GACGATTGGG ATGATGGCCT GACCTTCACC
    GCCAAATCAT CACCTGACTC GGATCGTGCC AATAACAATT CTCTGATAGC CCACATCCAA
    AACCTGCCGG GTTTCGATGC CTCCAAGGCC CGTATCGTCG TCCAGCACTA TGCGCCCAAG
    GCCGACGACA ATCCGGACAC AGATATAGAG TCCCCGGAGC AGGCCATTGG GCCCACGGCC
    GCCGAATTGA TATTGCCGTG GCGGCGGGAT CGTTCATACC AGAGCATTGT GGCCGAACAT
    CCCATTCCCG AGGAGGATCG TAATCTGTCC CGCGAATCCG ACGGAGAAAG GCGTGTGGCC
    ACACCCACCG CCACGGAATC CCAATTGCCG TGGAAAAGAC AGGGTGACGA ATGCACGGAT
    GCAGTGCAGC AGAACGAGTT TCCCGCTTGG GCCTCCAACA AGGAGTACTT GGCTTACAAT
    TCCCCCAGTG CAACATTCCT AGGTGGTATA AACAAGCCTA AACAGCCCAA GTCCGTCATA
    GGTCTCTTCC GCCGTGAGAG TTCCAGCTCG AAGGCCGGGA GCGTTGGCGT CGGCAGCACA
    GGAGCCATGG ACTCCGCCGC CAGTGGCTCC GAAACGATGG TGGTGTCCAT GTCCAGCCAA
    CCGCCCAACG CTCCATCGAC GTCCATCCAC AATCCGCGGC TGGACAAGCG CTCCCAGTCG
    ATCTCCTCAA GCTCGCTGGG CGCCGGAGCC CATCAGCTAG GTCCGGAGGG TCATTCCGGC
    CCATTTCCGG TTACGGCCAG TCATCGGCGG AACGTGCGCA GGGGCTCCAT GCTGGAGCTA
    AGCGGAGACA CAATACCGGA GGAGTCGTCG TACCAGCACG GACACTCCAA GTCCTTGTGC
    GAGCCGGCGG ACTCCGATGA ATGGGAGGGA GCACCGTTGT CCGCAGCCGG CGGAGCAAAC
    AGCGAGCTTT TGATGCGAAT GAGCGGCAGG GAACCACTCC TGCCACGCCC ATCCAACACG
    CCTCGTGCCC AGATTCGGCG CATGAACGCC GGGGCGGTGG GCGGTGCAGG CGTGAGCCAG
    GCGGGCCGTA AGAACCAGGT GACCAAGGCA CTGTTGGACT ACGAGGATTC CGAAACCGAC
    TCCGATGAGA ACGATGATGA TGAGGACGAG GACTTCGATT CCTACGATGA CGAGAACATT
    GTGGTCACCA CCTTTACAAC ACCGGCCACG GGCAGAAGAC CGGGATCTAG TCCAGGATCC
    GGGTCAGAAG CCAACACCGC CACTACCACC ACGACAAGCA TTCGGCTGAC CCGCAACAAC
    GACGAAAGCA TCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260660.1
    CDS132..4049
    Translation

    Target ORF information:

    RefSeq Version NM_001273731.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 2 (Nhe2), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273731.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGCATCC GCACGGAGCA GGATTACGAC AGTGCCACTC CGGCGCTGGC ACAGCAAATG 
    AATTTGGCCC GAAGAGCCTG CTGGAGGATC AAATCCTACA GCTCGGAATC CCTCTTCAAG
    ACCTATGCTT CTGTAATAAC AGATACTAGT GCAAATGAAA TCGATGCAGA AGCACCTCCA
    CCACCGAGAG ATAAAACCAA AACGAGAATA GAGCCACAGA TAGCGCCCGC AAAACGCAAC
    TCATCCTGCA CATCATCCGA CTGGCGGGGG ATGTTCAGCA AAAGGACGCT GCTCATCTGC
    GCCCTGGCAC TGATCCTTGG AAGTGCCCAG GCGCGGCCCA ACACGAGTGC TGTGGGAGTG
    GCTCCGAGAA AGGTCAGCCA GGACATTGTG GATGCGGTCA CCCAGCTAAA TCTGGGCCAG
    TCGGCGCCCA TTGATGCGGT GGATGTGGGG CTGGATCCAA CGCCATCGGC GAGAGTGCCA
    CGTCCCGAAC CATTGAAATC CGGCGACGAG AATGCGAAGG GCGACGAGGG ACACAAGATG
    GAGCGGTATC CCCTCTCCAG TGTGGACTTC GCTCGGGTAA AGACGCCGTT CATCATTGGA
    ATCTGGATCC TGTCGGCCAG TATAGCTAAA ATCGGTTTCC ATATGACGCC CAAACTGCAC
    CTAATATTTC CGGAATCGTG CCTGCTGATT GTCGTGGGCG TGGTCATTGG CGTGGTGCTC
    TATTTCTGCA CCGATGTCGC CGTCTCCCCG CTGACTCCCA ATACCTTCTT CTTCTACATG
    CTTCCACCGA TTATCCTGGA CGCGGGCTAC TTTATGCCCA ACAGATTGTT CTTCGACAAC
    CTGGGCACCA TCCTGCTGAT GGCGGTGGTC GGAACCATCT TCAACATAGC TACCATCGGT
    GGCTCCTTGT ACGCCTGCGG AAAGATGGGA ATTTACGGGG AAAGCGAGAC TCCGGGTCTA
    ATGGACGTGT TTCTATTTGC CTCACTGATA TCTGCAGTGG ATCCGGTGGC CGTATTGGCC
    GTATTCGAGG AGATACACGT GAACGAGATC CTATACATTG TCGTCTTTGG CGAGTCCTTG
    CTGAACGATG CCGTCACGGT TGTGATGTAC CACATGATGG AGTCCTACAA TGAGATTGGC
    TTGGACAAAA TCATCGCCCA GGACATTGCC AGCGGTGTGG GTTCCTTCTT CGTGGTAGCG
    CTCGGTGGCA CTGCCATAGG CATCATCTGG GGCTTTCTCA CGGGCTTGGT GACCCGATTC
    ACCGATCACG TGCGTGTCAT AGAACCCATT TTCATATTTG TGATGGCGTA CTTGGCCTAT
    CTCAATGCGG AAATATTCCA CATGAGCGGC ATTTTAGCCA TCACTTTCTG TGGTATCACA
    ATGAAAAACT ATGTGGAATC TAATATTTCA CAAAAGTCGC ATACGACTGT CAAATATGCC
    TTAAAGATGT TGTCCAGCTC GGCGGAGACC ATTATATTTA TGTTCCTAGG CGTGGCCACT
    GTGAACAACA TGCACGTATG GAATACGTGG TTTGTAGTGC TGACCATTGC CTTCTGTTCA
    GTATTTCGTG TCATTGGCGT CATTTTGCTA TCGGCCCTTG CCAATCGCTT CCGCTTGCAC
    AAATTGTCCC GGGTGGATCA GTTTGTGATG TCCTACGGAG GATTGCGTGG TGCTGTGGCC
    TTTGCCCTGG TGCTGTTGGT GGACGAGAAT GTGGTTAAGC AGAAGAACAT GTTTGTTACC
    ACCACGATAG CTGTGATTTA CTTTACTGTC TTCCTGCAAG GAATCACTAT AAAGCCGCTG
    GTGAAAATCC TTAATGTCAA GCGCGCTAAT AAACGCAAGC CAACCATGAA TGAGCGCATT
    CATGAACGGT TCATGGATCA CTTGATGGCT GGAATTGAGG ATATAGTGGG CAAGACGGGC
    AACTACAACG TGCGTGACAA GTTCAAGCGT TTCGACAATC GCTTTATTCG CCCGCTGCTG
    ATCAGAGATC TTAAGGGAGC TGAGCCGAAG ATCATCGAGA CGTACTCCAA ACTGACGATG
    CGCGATGCTA TGGAAGTTAT GAGGCGAAAT CCATCCACTA TTGGCCAGAT GACGGGAACC
    GAGTCGATGA GCGCCCTTTT CCGGAATTAT ACCAATAACT ATATTGGCGG CAGGTGGGCA
    CCGCCAACCA TATACACCAC CTGCCCCAGT CTGACAAATC TAGACAATAC CTGTTCCCGT
    AATCTGGACA TGGCTGAGCT GGATTATAAT CCATCCAAGA AGGATCTAAC CGATGCCAGG
    ATCCATCATC TTTTGGCCGA AGAACTGAAG CCTTATAGAA GGGCCTCAAT ACAAATGCAC
    CGTCGTCTTA GTTATAGCCG ACACGCAGTA GATGACAGAG ATTTGTCAAC CCAGGTCAAT
    TACAAGATGC AAATGAACTT CAGGCGGATG TTTAATGATC GAAAACATCA CAAACGCAGC
    AAACGTGGTG CCAGCAACAA GGCCAAGGAG AACGTTAAGC AGAATCATGT CTCGTTCCAT
    GACTTTCAAC AGAACGGCAC CACTAAGCAA CTCACCAATG CCGAGGAGTG CCAACAGAAT
    CCCAACGAGA TCAATGTTGT TGGCCCGAGC GACGATTGGG ATGATGGCCT GACCTTCACC
    GCCAAATCAT CACCTGACTC GGATCGTGCC AATAACAATT CTCTGATAGC CCACATCCAA
    AACCTGCCGG GTTTCGATGC CTCCAAGGCC CGTATCGTCG TCCAGCACTA TGCGCCCAAG
    GCCGACGACA ATCCGGACAC AGATATAGAG TCCCCGGAGC AGGCCATTGG GCCCACGGCC
    GCCGAATTGA TATTGCCGTG GCGGCGGGAT CGTTCATACC AGAGCATTGT GGCCGAACAT
    CCCATTCCCG AGGAGGATCG TAATCTGTCC CGCGAATCCG ACGGAGAAAG GCGTGTGGCC
    ACACCCACCG CCACGGAATC CCAATTGCCG TGGAAAAGAC AGGGTGACGA ATGCACGGAT
    GCAGTGCAGC AGAACGAGTT TCCCGCTTGG GCCTCCAACA AGGAGTACTT GGCTTACAAT
    TCCCCCAGTG CAACATTCCT AGGTGGTATA AACAAGCCTA AACAGCCCAA GTCCGTCATA
    GGTCTCTTCC GCCGTGAGAG TTCCAGCTCG AAGGCCGGGA GCGTTGGCGT CGGCAGCACA
    GGAGCCATGG ACTCCGCCGC CAGTGGCTCC GAAACGATGG TGGTGTCCAT GTCCAGCCAA
    CCGCCCAACG CTCCATCGAC GTCCATCCAC AATCCGCGGC TGGACAAGCG CTCCCAGTCG
    ATCTCCTCAA GCTCGCTGGG CGCCGGAGCC CATCAGCTAG GTCCGGAGGG TCATTCCGGC
    CCATTTCCGG TTACGGCCAG TCATCGGCGG AACGTGCGCA GGGGCTCCAT GCTGGAGCTA
    AGCGGAGACA CAATACCGGA GGAGTCGTCG TACCAGCACG GACACTCCAA GTCCTTGTGC
    GAGCCGGCGG ACTCCGATGA ATGGGAGGGA GCACCGTTGT CCGCAGCCGG CGGAGCAAAC
    AGCGAGCTTT TGATGCGAAT GAGCGGCAGG GAACCACTCC TGCCACGCCC ATCCAACACG
    CCTCGTGCCC AGATTCGGCG CATGAACGCC GGGGCGGTGG GCGGTGCAGG CGTGAGCCAG
    GCGGGCCGTA AGAACCAGGT GACCAAGGCA CTGTTGGACT ACGAGGATTC CGAAACCGAC
    TCCGATGAGA ACGATGATGA TGAGGACGAG GACTTCGATT CCTACGATGA CGAGAACATT
    GTGGTCACCA CCTTTACAAC ACCGGCCACG GGCAGAAGAC CGGGATCTAG TCCAGGATCC
    GGGTCAGAAG CCAACACCGC CACTACCACC ACGACAAGCA TTCGGCTGAC CCGCAACAAC
    GACGAAAGCA TCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.