Fs(2)Ket cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fs(2)Ket
Entrez Gene ID	35336
Full Name	Female sterile (2) Ketel
Synonyms	38E.11,CG2637,Dmel\CG2637,Fs(2)Hon,Fs(2)Sz6,Fs(2)Sz7,Fs(2)ket,Hon,IMPbeta1,Impbeta,Ket,Ketel,Kpnb1,MRE26,dKap-beta,fs(2)Ket,fs(2)lo-B,fs(2)lto2,fs(2)ltoHD43,imp-beta,impbeta,ketel,kettle
General protein information	Preferred Names Female sterile (2) Ketel Names CG2637 gene product from transcript CG2637-RB CG2637-PA CG2637-PB CG2637-PC CG2637-PD CG2637-PE DImportin-beta Fs(2)Ket-PA Fs(2)Ket-PB Fs(2)Ket-PC Fs(2)Ket-PD Fs(2)Ket-PE Importin-beta female sterile (2) ketel female sterile(2)lto2 fs(2) ketel hont importin beta importin beta1 ketel mRNA-like ncRNA in embryogenesis 26
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38E5-38E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_058148.5	NP_477496.1	female sterile (2) ketel, isoform A
NM_001259182.2	NP_001246111.1	female sterile (2) ketel, isoform E
NM_001259179.2	NP_001246108.1	female sterile (2) ketel, isoform D
NM_001259180.2	NP_001246109.1	female sterile (2) ketel, isoform B
NM_001259181.2	NP_001246110.1	female sterile (2) ketel, isoform C

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	KPNB1	XP_004950071.1
Caenorhabditis elegans (roundworm)	imb-1	NP_491477.2
Kluyveromyces lactis	KLLA0B05665g	XP_451788.1
Homo sapiens (human)	KPNB1	NP_002256.2
Bos taurus (cattle)	KPNB1	NP_001180082.1
Mus musculus (house mouse)	Kpnb1	NP_032405.3
Drosophila melanogaster (fruit fly)	Fs(2)Ket	NP_001246109.1
Neurospora crassa	NCU02011	XP_964876.1
Oryza sativa (rice)	Os12g0568800	NP_001067068.1
Saccharomyces cerevisiae (baker's yeast)	KAP95	NP_013451.1
Schizosaccharomyces pombe (fission yeast)	kap95	NP_594233.1
Pan troglodytes (chimpanzee)	KPNB1	XP_003315429.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009921	XP_319040.3
Arabidopsis thaliana (thale cress)	AT5G53480	NP_200160.1
Xenopus tropicalis (tropical clawed frog)	kpnb1	XP_002940197.1
	KPNB1	XP_001082833.2
Canis lupus familiaris (dog)	KPNB1	XP_005624657.1
Rattus norvegicus (Norway rat)	Kpnb1	NP_058759.1
Danio rerio (zebrafish)	kpnb1	NP_001032791.2
Eremothecium gossypii	AGOS_ACL187W	NP_983217.2
Magnaporthe oryzae (rice blast fungus)	MGG_03668	XP_003716218.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nup358 facilitates JH-induced Met nuclear transport in a manner dependent on importin beta and Hsp83.
Title: Nucleoporin Nup358 facilitates nuclear import of Methoprene-tolerant (Met) in an importin ?- and Hsp83-dependent manner.

The regulation of Ketel gene expression depends largely on a DRE motif and action of the DREF, CFDD, CF2-II and BEAF transcription factors.
Title: The DRE motif is a key component in the expression regulation of the importin-beta encoding Ketel gene in Drosophila.

Data show that the P446L mutant of Drosophila importin beta interferes with nuclear envelope formation during mitosis-to-interphase transition.
Title: The importin-beta P446L dominant-negative mutant protein loses RanGTP binding ability and blocks the formation of intact nuclear envelope.

The following Fs(2)Ket gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fs(2)Ket cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15055	NM_001259179.2 Latest version!	Drosophila melanogaster female sterile (2) ketel, transcript variant D (Fs(2)Ket), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa15055	NM_001259181.2 Latest version!	Drosophila melanogaster female sterile (2) ketel, transcript variant C (Fs(2)Ket), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa15055	NM_001259182.2 Latest version!	Drosophila melanogaster female sterile (2) ketel, transcript variant E (Fs(2)Ket), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa15055	NM_001259180.2 Latest version!	Drosophila melanogaster female sterile (2) ketel, transcript variant B (Fs(2)Ket), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa15055	NM_058148.5 Latest version!	Drosophila melanogaster female sterile (2) ketel, transcript variant A (Fs(2)Ket), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fs(2)Ket ( NM_058148.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477496.1 Drosophila melanogaster female sterile (2) ketel, transcript variant A (Fs(2)Ket), mRNA.

Price & Availability↑

CloneID	OFa15055
Clone ID Related Accession (Same CDS sequence)	NM_058148.5 , NM_001259182.2 , NM_001259179.2 , NM_001259180.2 , NM_001259181.2
Accession Version	NM_058148.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	female sterile (2) ketel, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_058148.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTTCCG ATATCGCCAT GCAATTGATT GCCATTTTGG AAAAGACGGT GTCGCCAGAC 
AAAAATGAGC TGCTATCGGC GAAGAACTTT CTGGAGCAGG CGGCGGCCAG CAACCTGCCC 
GAGTTTCTCA AGGCGCTGTC AGAGATACTG GTGAACACGG CCAACAGTGC GGTGGCGCGA 
ATGGCAGCCG GTCTCCAGCT GAAGAACCAC CTGACCAGCA AGGACGAGAA GGTCAGCCAA 
CAGTACCAGG ATCGCTGGCA TCAGTTTCCC AGCGAGATCC GCGAGTTGAT CAAGAATAAC 
ATCCTGGCTG CTTTGGGTAC CGAGAACACC CGACCCTCCT GCGCCGCCCA GTGCGTGGCC 
TATGTGGCCG TGATTGAGCT GCCGATAAAC CGCTGGCCCA TGCTCATCCA GACACTGGTG 
AACAAGGTGG TCAGCGAAGG ATCCAGCGAG ATGCATCGCG AGTCGGCGCT GGAGGCCATT 
GGCTACATTT GCCAGGACAT TCGTTTCGGC GTCATGGAGA ACCAGTCGAA CGATGTGCTG 
ACTGCTATTA TTCACGGCAT GAGAAAGGTA GAGCCGAGCA ATCATGTACG CTTGGCGGCC 
ACCACGGCCC TTCACAACTC GTTGGAGTTC ACCAAATCGA ACTTTGAGAA AGACATGGAG 
CGCAACTTCA TCATGGAGGT GGTGTGCGAG GCCACTCAGT GCCAGGACTC GCAAATCTGC 
GTTGCCGCTC TGCAGTGCCT GGTGAAGATT ATGACCCTTT ATTACCAGTA CATGGAGCCC 
TACATGGCAC AGGCTCTTTT CCCTATTACT TTGGCGGCCA TGAAGTCTGA TAACGATGCT 
GTAGCGCTGC AGGGCATAGA GTTCTGGTCG AACGTCTGCG ATGAGGAGAT AGATCTGGCC 
ATCGAAAGCC AGGAGGCCAC TGATCAGGGC CGCGCTCCGC AGAGAGTGTC CAAGCACTAT 
GCTCGAGGTG CTCTGCAGTT CCTGACGCCT GTGCTCGTCG AGAAATTAAC CAAGCAGGAC 
GAGTGCGATG ACGAGGACAC CTGGAGCCCG GCGAAGGCCG CCTCCGTTTG CCTCATGGTG 
CTGGCCACAT GTTGCGAGGA CGAGATCGTG CCACATGTGC TTCCCTTCAT CAAGGAGAAC 
ATCGAGTCAC CTAATTGGCG ATTCCGCGAC GCAGCGGTAA TGACTTTCGG CTCCGTGCTG 
AATGGATTGG AGACCAATAC CTTAAAGCCG CTCGTGGAGC AAGCCATGCC CACGCTCATT 
CGACTGATGT ACGACTCCAG CGTTATCGTG CGCGATACCA TCGCCTGGAC CTTCGGACGT 
ATTTGCGATA TAATTCCCGA GGCGGCTATC AACGAGACAT ATCTTCAAAC GCTTTTGGAG 
TGCTTCGTGA AGAGCCTAAA GTCAGAGCCG CGTGTAGCAG CCAACGTGTG TTGGGCCTTC 
ATTGGACTTT CCGATGCAGC TTGGGAGGCA GCGGTCACCA ACGACGGAGA GACTCCGGAG 
ACGTACGCCC TGTCCCCGTA CTTTGAGTAC ATCATTACCC AGTTGCTGGA GACCACTGAT 
CGATCGGACG GAGCTCAAGC CAATTTGCGA TGTGCTGCTT ATCAGGCACT TATGGATATG 
ATCAAGAACT CTCCCTTGGA TTGCTACTTG GTGGTCCAGC GCACCACGCT GGTGATTTTG 
GAGCGCTTGA ACCAGGTGAT GCAGATGGAA ACGCAGATTA ACAATCACAG CGATCGTCAT 
CAGTTCAACG ATCTACAATC GCTTCTGTGC GCCACTCTGC AAAGCGTTTT GAGAAAGGTC 
CACGAGCAGG ATGCGCCTCA AATTTCCGAT GCCATTATGA CCGCTCTGCT GACCATGTTC 
AATTCAAGTG CTGGAAAGTC GGGTGTAGTG CAGGAGGAGG CCTTCCTTGC CGTTTCGACA 
TTGGTTGAGC TTTTGGGAGC TCAGTTCGCC AAGTACATGC CCGCTTTCAA GGACTTCCTC 
GTCATGGGTC TGAAGAACTT CCAGGAGTAC CAGGTCTGCT GCGCAGCTGT TGGTCTCACC 
GGCGACATTT TCCGCGCCCT TAAGGACCTA ATGGTGCCCT ATTCCAACGA AATTATGACC 
GTATTGATAA ACAATCTAAC GGAGCCGACT ATCCATCGAA CCGTCAAGCC CCAGGTTCTG 
TCTGCTTTCG GAGATATTGC GTTGAGCATT GGCAACCACT TCCTGCCATA TTTGAGCATG 
GTCCTAGATA TGTTGCGCGT TGCGTCCAAT CTGCAGACGG ACGCCAACAA CTTCGATATG 
AACGAATACA TCAACGAGCT GCGTGAAAGC ATCCTTGAAG CCTACACTGG CATCATCCAG 
GGCCTTAAGG GCGTTGACCA GACTGCCCAT ACAGACGTTA TGCACATGGA GCCCCACCTG 
ATGCACATTA TTTCGTTCAT CAAGCGCATC GCTCAGGAGG GCGACGTGTC CGACTCAATG 
TTGGCCAGTG CCGCTGGCTT CATTGGCGAT CTGTGCACTT CGTTTGGTCC GCGGCTATAC 
CCGCTGCTGG ATGATGCCAT CATTACCCAA TTCCTTGCCG AGGGCAAGCG CTCCAAGGCG 
CAGCGCACCA AGATGCTGTG CACCTGGGCC GTCAAAGAGA TAAAAAAAAT CAATACCCAG 
GTCATCACGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477496.1
CDS	301..2955
Translation	MTSDIAMQLIAILEKTVSPDKNELLSAKNFLEQAAASNLPEFLKALSEILVNTANSAVARMAAGLQLKNHLTSKDEKVSQQYQDRWHQFPSEIRELIKNNILAALGTENTRPSCAAQCVAYVAVIELPINRWPMLIQTLVNKVVSEGSSEMHRESALEAIGYICQDIRFGVMENQSNDVLTAIIHGMRKVEPSNHVRLAATTALHNSLEFTKSNFEKDMERNFIMEVVCEATQCQDSQICVAALQCLVKIMTLYYQYMEPYMAQALFPITLAAMKSDNDAVALQGIEFWSNVCDEEIDLAIESQEATDQGRAPQRVSKHYARGALQFLTPVLVEKLTKQDECDDEDTWSPAKAASVCLMVLATCCEDEIVPHVLPFIKENIESPNWRFRDAAVMTFGSVLNGLETNTLKPLVEQAMPTLIRLMYDSSVIVRDTIAWTFGRICDIIPEAAINETYLQTLLECFVKSLKSEPRVAANVCWAFIGLSDAAWEAAVTNDGETPETYALSPYFEYIITQLLETTDRSDGAQANLRCAAYQALMDMIKNSPLDCYLVVQRTTLVILERLNQVMQMETQINNHSDRHQFNDLQSLLCATLQSVLRKVHEQDAPQISDAIMTALLTMFNSSAGKSGVVQEEAFLAVSTLVELLGAQFAKYMPAFKDFLVMGLKNFQEYQVCCAAVGLTGDIFRALKDLMVPYSNEIMTVLINNLTEPTIHRTVKPQVLSAFGDIALSIGNHFLPYLSMVLDMLRVASNLQTDANNFDMNEYINELRESILEAYTGIIQGLKGVDQTAHTDVMHMEPHLMHIISFIKRIAQEGDVSDSMLASAAGFIGDLCTSFGPRLYPLLDDAIITQFLAEGKRSKAQRTKMLCTWAVKEIKKINTQVITQ

Target ORF information:

RefSeq Version	NM_058148.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster female sterile (2) ketel, transcript variant A (Fs(2)Ket), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058148.5

ORF Insert Sequence:

ATGACTTCCG ATATCGCCAT GCAATTGATT GCCATTTTGG AAAAGACGGT GTCGCCAGAC 
AAAAATGAGC TGCTATCGGC GAAGAACTTT CTGGAGCAGG CGGCGGCCAG CAACCTGCCC 
GAGTTTCTCA AGGCGCTGTC AGAGATACTG GTGAACACGG CCAACAGTGC GGTGGCGCGA 
ATGGCAGCCG GTCTCCAGCT GAAGAACCAC CTGACCAGCA AGGACGAGAA GGTCAGCCAA 
CAGTACCAGG ATCGCTGGCA TCAGTTTCCC AGCGAGATCC GCGAGTTGAT CAAGAATAAC 
ATCCTGGCTG CTTTGGGTAC CGAGAACACC CGACCCTCCT GCGCCGCCCA GTGCGTGGCC 
TATGTGGCCG TGATTGAGCT GCCGATAAAC CGCTGGCCCA TGCTCATCCA GACACTGGTG 
AACAAGGTGG TCAGCGAAGG ATCCAGCGAG ATGCATCGCG AGTCGGCGCT GGAGGCCATT 
GGCTACATTT GCCAGGACAT TCGTTTCGGC GTCATGGAGA ACCAGTCGAA CGATGTGCTG 
ACTGCTATTA TTCACGGCAT GAGAAAGGTA GAGCCGAGCA ATCATGTACG CTTGGCGGCC 
ACCACGGCCC TTCACAACTC GTTGGAGTTC ACCAAATCGA ACTTTGAGAA AGACATGGAG 
CGCAACTTCA TCATGGAGGT GGTGTGCGAG GCCACTCAGT GCCAGGACTC GCAAATCTGC 
GTTGCCGCTC TGCAGTGCCT GGTGAAGATT ATGACCCTTT ATTACCAGTA CATGGAGCCC 
TACATGGCAC AGGCTCTTTT CCCTATTACT TTGGCGGCCA TGAAGTCTGA TAACGATGCT 
GTAGCGCTGC AGGGCATAGA GTTCTGGTCG AACGTCTGCG ATGAGGAGAT AGATCTGGCC 
ATCGAAAGCC AGGAGGCCAC TGATCAGGGC CGCGCTCCGC AGAGAGTGTC CAAGCACTAT 
GCTCGAGGTG CTCTGCAGTT CCTGACGCCT GTGCTCGTCG AGAAATTAAC CAAGCAGGAC 
GAGTGCGATG ACGAGGACAC CTGGAGCCCG GCGAAGGCCG CCTCCGTTTG CCTCATGGTG 
CTGGCCACAT GTTGCGAGGA CGAGATCGTG CCACATGTGC TTCCCTTCAT CAAGGAGAAC 
ATCGAGTCAC CTAATTGGCG ATTCCGCGAC GCAGCGGTAA TGACTTTCGG CTCCGTGCTG 
AATGGATTGG AGACCAATAC CTTAAAGCCG CTCGTGGAGC AAGCCATGCC CACGCTCATT 
CGACTGATGT ACGACTCCAG CGTTATCGTG CGCGATACCA TCGCCTGGAC CTTCGGACGT 
ATTTGCGATA TAATTCCCGA GGCGGCTATC AACGAGACAT ATCTTCAAAC GCTTTTGGAG 
TGCTTCGTGA AGAGCCTAAA GTCAGAGCCG CGTGTAGCAG CCAACGTGTG TTGGGCCTTC 
ATTGGACTTT CCGATGCAGC TTGGGAGGCA GCGGTCACCA ACGACGGAGA GACTCCGGAG 
ACGTACGCCC TGTCCCCGTA CTTTGAGTAC ATCATTACCC AGTTGCTGGA GACCACTGAT 
CGATCGGACG GAGCTCAAGC CAATTTGCGA TGTGCTGCTT ATCAGGCACT TATGGATATG 
ATCAAGAACT CTCCCTTGGA TTGCTACTTG GTGGTCCAGC GCACCACGCT GGTGATTTTG 
GAGCGCTTGA ACCAGGTGAT GCAGATGGAA ACGCAGATTA ACAATCACAG CGATCGTCAT 
CAGTTCAACG ATCTACAATC GCTTCTGTGC GCCACTCTGC AAAGCGTTTT GAGAAAGGTC 
CACGAGCAGG ATGCGCCTCA AATTTCCGAT GCCATTATGA CCGCTCTGCT GACCATGTTC 
AATTCAAGTG CTGGAAAGTC GGGTGTAGTG CAGGAGGAGG CCTTCCTTGC CGTTTCGACA 
TTGGTTGAGC TTTTGGGAGC TCAGTTCGCC AAGTACATGC CCGCTTTCAA GGACTTCCTC 
GTCATGGGTC TGAAGAACTT CCAGGAGTAC CAGGTCTGCT GCGCAGCTGT TGGTCTCACC 
GGCGACATTT TCCGCGCCCT TAAGGACCTA ATGGTGCCCT ATTCCAACGA AATTATGACC 
GTATTGATAA ACAATCTAAC GGAGCCGACT ATCCATCGAA CCGTCAAGCC CCAGGTTCTG 
TCTGCTTTCG GAGATATTGC GTTGAGCATT GGCAACCACT TCCTGCCATA TTTGAGCATG 
GTCCTAGATA TGTTGCGCGT TGCGTCCAAT CTGCAGACGG ACGCCAACAA CTTCGATATG 
AACGAATACA TCAACGAGCT GCGTGAAAGC ATCCTTGAAG CCTACACTGG CATCATCCAG 
GGCCTTAAGG GCGTTGACCA GACTGCCCAT ACAGACGTTA TGCACATGGA GCCCCACCTG 
ATGCACATTA TTTCGTTCAT CAAGCGCATC GCTCAGGAGG GCGACGTGTC CGACTCAATG 
TTGGCCAGTG CCGCTGGCTT CATTGGCGAT CTGTGCACTT CGTTTGGTCC GCGGCTATAC 
CCGCTGCTGG ATGATGCCAT CATTACCCAA TTCCTTGCCG AGGGCAAGCG CTCCAAGGCG 
CAGCGCACCA AGATGCTGTG CACCTGGGCC GTCAAAGAGA TAAAAAAAAT CAATACCCAG 
GTCATCACGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fs(2)Ket ( NM_001259182.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246111.1 Drosophila melanogaster female sterile (2) ketel, transcript variant E (Fs(2)Ket), mRNA.

Price & Availability↑

CloneID	OFa15055
Clone ID Related Accession (Same CDS sequence)	NM_058148.5 , NM_001259182.2 , NM_001259179.2 , NM_001259180.2 , NM_001259181.2
Accession Version	NM_001259182.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	female sterile (2) ketel, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259182.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTTCCG ATATCGCCAT GCAATTGATT GCCATTTTGG AAAAGACGGT GTCGCCAGAC 
AAAAATGAGC TGCTATCGGC GAAGAACTTT CTGGAGCAGG CGGCGGCCAG CAACCTGCCC 
GAGTTTCTCA AGGCGCTGTC AGAGATACTG GTGAACACGG CCAACAGTGC GGTGGCGCGA 
ATGGCAGCCG GTCTCCAGCT GAAGAACCAC CTGACCAGCA AGGACGAGAA GGTCAGCCAA 
CAGTACCAGG ATCGCTGGCA TCAGTTTCCC AGCGAGATCC GCGAGTTGAT CAAGAATAAC 
ATCCTGGCTG CTTTGGGTAC CGAGAACACC CGACCCTCCT GCGCCGCCCA GTGCGTGGCC 
TATGTGGCCG TGATTGAGCT GCCGATAAAC CGCTGGCCCA TGCTCATCCA GACACTGGTG 
AACAAGGTGG TCAGCGAAGG ATCCAGCGAG ATGCATCGCG AGTCGGCGCT GGAGGCCATT 
GGCTACATTT GCCAGGACAT TCGTTTCGGC GTCATGGAGA ACCAGTCGAA CGATGTGCTG 
ACTGCTATTA TTCACGGCAT GAGAAAGGTA GAGCCGAGCA ATCATGTACG CTTGGCGGCC 
ACCACGGCCC TTCACAACTC GTTGGAGTTC ACCAAATCGA ACTTTGAGAA AGACATGGAG 
CGCAACTTCA TCATGGAGGT GGTGTGCGAG GCCACTCAGT GCCAGGACTC GCAAATCTGC 
GTTGCCGCTC TGCAGTGCCT GGTGAAGATT ATGACCCTTT ATTACCAGTA CATGGAGCCC 
TACATGGCAC AGGCTCTTTT CCCTATTACT TTGGCGGCCA TGAAGTCTGA TAACGATGCT 
GTAGCGCTGC AGGGCATAGA GTTCTGGTCG AACGTCTGCG ATGAGGAGAT AGATCTGGCC 
ATCGAAAGCC AGGAGGCCAC TGATCAGGGC CGCGCTCCGC AGAGAGTGTC CAAGCACTAT 
GCTCGAGGTG CTCTGCAGTT CCTGACGCCT GTGCTCGTCG AGAAATTAAC CAAGCAGGAC 
GAGTGCGATG ACGAGGACAC CTGGAGCCCG GCGAAGGCCG CCTCCGTTTG CCTCATGGTG 
CTGGCCACAT GTTGCGAGGA CGAGATCGTG CCACATGTGC TTCCCTTCAT CAAGGAGAAC 
ATCGAGTCAC CTAATTGGCG ATTCCGCGAC GCAGCGGTAA TGACTTTCGG CTCCGTGCTG 
AATGGATTGG AGACCAATAC CTTAAAGCCG CTCGTGGAGC AAGCCATGCC CACGCTCATT 
CGACTGATGT ACGACTCCAG CGTTATCGTG CGCGATACCA TCGCCTGGAC CTTCGGACGT 
ATTTGCGATA TAATTCCCGA GGCGGCTATC AACGAGACAT ATCTTCAAAC GCTTTTGGAG 
TGCTTCGTGA AGAGCCTAAA GTCAGAGCCG CGTGTAGCAG CCAACGTGTG TTGGGCCTTC 
ATTGGACTTT CCGATGCAGC TTGGGAGGCA GCGGTCACCA ACGACGGAGA GACTCCGGAG 
ACGTACGCCC TGTCCCCGTA CTTTGAGTAC ATCATTACCC AGTTGCTGGA GACCACTGAT 
CGATCGGACG GAGCTCAAGC CAATTTGCGA TGTGCTGCTT ATCAGGCACT TATGGATATG 
ATCAAGAACT CTCCCTTGGA TTGCTACTTG GTGGTCCAGC GCACCACGCT GGTGATTTTG 
GAGCGCTTGA ACCAGGTGAT GCAGATGGAA ACGCAGATTA ACAATCACAG CGATCGTCAT 
CAGTTCAACG ATCTACAATC GCTTCTGTGC GCCACTCTGC AAAGCGTTTT GAGAAAGGTC 
CACGAGCAGG ATGCGCCTCA AATTTCCGAT GCCATTATGA CCGCTCTGCT GACCATGTTC 
AATTCAAGTG CTGGAAAGTC GGGTGTAGTG CAGGAGGAGG CCTTCCTTGC CGTTTCGACA 
TTGGTTGAGC TTTTGGGAGC TCAGTTCGCC AAGTACATGC CCGCTTTCAA GGACTTCCTC 
GTCATGGGTC TGAAGAACTT CCAGGAGTAC CAGGTCTGCT GCGCAGCTGT TGGTCTCACC 
GGCGACATTT TCCGCGCCCT TAAGGACCTA ATGGTGCCCT ATTCCAACGA AATTATGACC 
GTATTGATAA ACAATCTAAC GGAGCCGACT ATCCATCGAA CCGTCAAGCC CCAGGTTCTG 
TCTGCTTTCG GAGATATTGC GTTGAGCATT GGCAACCACT TCCTGCCATA TTTGAGCATG 
GTCCTAGATA TGTTGCGCGT TGCGTCCAAT CTGCAGACGG ACGCCAACAA CTTCGATATG 
AACGAATACA TCAACGAGCT GCGTGAAAGC ATCCTTGAAG CCTACACTGG CATCATCCAG 
GGCCTTAAGG GCGTTGACCA GACTGCCCAT ACAGACGTTA TGCACATGGA GCCCCACCTG 
ATGCACATTA TTTCGTTCAT CAAGCGCATC GCTCAGGAGG GCGACGTGTC CGACTCAATG 
TTGGCCAGTG CCGCTGGCTT CATTGGCGAT CTGTGCACTT CGTTTGGTCC GCGGCTATAC 
CCGCTGCTGG ATGATGCCAT CATTACCCAA TTCCTTGCCG AGGGCAAGCG CTCCAAGGCG 
CAGCGCACCA AGATGCTGTG CACCTGGGCC GTCAAAGAGA TAAAAAAAAT CAATACCCAG 
GTCATCACGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246111.1
CDS	301..2955
Translation	MTSDIAMQLIAILEKTVSPDKNELLSAKNFLEQAAASNLPEFLKALSEILVNTANSAVARMAAGLQLKNHLTSKDEKVSQQYQDRWHQFPSEIRELIKNNILAALGTENTRPSCAAQCVAYVAVIELPINRWPMLIQTLVNKVVSEGSSEMHRESALEAIGYICQDIRFGVMENQSNDVLTAIIHGMRKVEPSNHVRLAATTALHNSLEFTKSNFEKDMERNFIMEVVCEATQCQDSQICVAALQCLVKIMTLYYQYMEPYMAQALFPITLAAMKSDNDAVALQGIEFWSNVCDEEIDLAIESQEATDQGRAPQRVSKHYARGALQFLTPVLVEKLTKQDECDDEDTWSPAKAASVCLMVLATCCEDEIVPHVLPFIKENIESPNWRFRDAAVMTFGSVLNGLETNTLKPLVEQAMPTLIRLMYDSSVIVRDTIAWTFGRICDIIPEAAINETYLQTLLECFVKSLKSEPRVAANVCWAFIGLSDAAWEAAVTNDGETPETYALSPYFEYIITQLLETTDRSDGAQANLRCAAYQALMDMIKNSPLDCYLVVQRTTLVILERLNQVMQMETQINNHSDRHQFNDLQSLLCATLQSVLRKVHEQDAPQISDAIMTALLTMFNSSAGKSGVVQEEAFLAVSTLVELLGAQFAKYMPAFKDFLVMGLKNFQEYQVCCAAVGLTGDIFRALKDLMVPYSNEIMTVLINNLTEPTIHRTVKPQVLSAFGDIALSIGNHFLPYLSMVLDMLRVASNLQTDANNFDMNEYINELRESILEAYTGIIQGLKGVDQTAHTDVMHMEPHLMHIISFIKRIAQEGDVSDSMLASAAGFIGDLCTSFGPRLYPLLDDAIITQFLAEGKRSKAQRTKMLCTWAVKEIKKINTQVITQ

Target ORF information:

RefSeq Version	NM_001259182.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster female sterile (2) ketel, transcript variant E (Fs(2)Ket), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259182.2

ORF Insert Sequence:

ATGACTTCCG ATATCGCCAT GCAATTGATT GCCATTTTGG AAAAGACGGT GTCGCCAGAC 
AAAAATGAGC TGCTATCGGC GAAGAACTTT CTGGAGCAGG CGGCGGCCAG CAACCTGCCC 
GAGTTTCTCA AGGCGCTGTC AGAGATACTG GTGAACACGG CCAACAGTGC GGTGGCGCGA 
ATGGCAGCCG GTCTCCAGCT GAAGAACCAC CTGACCAGCA AGGACGAGAA GGTCAGCCAA 
CAGTACCAGG ATCGCTGGCA TCAGTTTCCC AGCGAGATCC GCGAGTTGAT CAAGAATAAC 
ATCCTGGCTG CTTTGGGTAC CGAGAACACC CGACCCTCCT GCGCCGCCCA GTGCGTGGCC 
TATGTGGCCG TGATTGAGCT GCCGATAAAC CGCTGGCCCA TGCTCATCCA GACACTGGTG 
AACAAGGTGG TCAGCGAAGG ATCCAGCGAG ATGCATCGCG AGTCGGCGCT GGAGGCCATT 
GGCTACATTT GCCAGGACAT TCGTTTCGGC GTCATGGAGA ACCAGTCGAA CGATGTGCTG 
ACTGCTATTA TTCACGGCAT GAGAAAGGTA GAGCCGAGCA ATCATGTACG CTTGGCGGCC 
ACCACGGCCC TTCACAACTC GTTGGAGTTC ACCAAATCGA ACTTTGAGAA AGACATGGAG 
CGCAACTTCA TCATGGAGGT GGTGTGCGAG GCCACTCAGT GCCAGGACTC GCAAATCTGC 
GTTGCCGCTC TGCAGTGCCT GGTGAAGATT ATGACCCTTT ATTACCAGTA CATGGAGCCC 
TACATGGCAC AGGCTCTTTT CCCTATTACT TTGGCGGCCA TGAAGTCTGA TAACGATGCT 
GTAGCGCTGC AGGGCATAGA GTTCTGGTCG AACGTCTGCG ATGAGGAGAT AGATCTGGCC 
ATCGAAAGCC AGGAGGCCAC TGATCAGGGC CGCGCTCCGC AGAGAGTGTC CAAGCACTAT 
GCTCGAGGTG CTCTGCAGTT CCTGACGCCT GTGCTCGTCG AGAAATTAAC CAAGCAGGAC 
GAGTGCGATG ACGAGGACAC CTGGAGCCCG GCGAAGGCCG CCTCCGTTTG CCTCATGGTG 
CTGGCCACAT GTTGCGAGGA CGAGATCGTG CCACATGTGC TTCCCTTCAT CAAGGAGAAC 
ATCGAGTCAC CTAATTGGCG ATTCCGCGAC GCAGCGGTAA TGACTTTCGG CTCCGTGCTG 
AATGGATTGG AGACCAATAC CTTAAAGCCG CTCGTGGAGC AAGCCATGCC CACGCTCATT 
CGACTGATGT ACGACTCCAG CGTTATCGTG CGCGATACCA TCGCCTGGAC CTTCGGACGT 
ATTTGCGATA TAATTCCCGA GGCGGCTATC AACGAGACAT ATCTTCAAAC GCTTTTGGAG 
TGCTTCGTGA AGAGCCTAAA GTCAGAGCCG CGTGTAGCAG CCAACGTGTG TTGGGCCTTC 
ATTGGACTTT CCGATGCAGC TTGGGAGGCA GCGGTCACCA ACGACGGAGA GACTCCGGAG 
ACGTACGCCC TGTCCCCGTA CTTTGAGTAC ATCATTACCC AGTTGCTGGA GACCACTGAT 
CGATCGGACG GAGCTCAAGC CAATTTGCGA TGTGCTGCTT ATCAGGCACT TATGGATATG 
ATCAAGAACT CTCCCTTGGA TTGCTACTTG GTGGTCCAGC GCACCACGCT GGTGATTTTG 
GAGCGCTTGA ACCAGGTGAT GCAGATGGAA ACGCAGATTA ACAATCACAG CGATCGTCAT 
CAGTTCAACG ATCTACAATC GCTTCTGTGC GCCACTCTGC AAAGCGTTTT GAGAAAGGTC 
CACGAGCAGG ATGCGCCTCA AATTTCCGAT GCCATTATGA CCGCTCTGCT GACCATGTTC 
AATTCAAGTG CTGGAAAGTC GGGTGTAGTG CAGGAGGAGG CCTTCCTTGC CGTTTCGACA 
TTGGTTGAGC TTTTGGGAGC TCAGTTCGCC AAGTACATGC CCGCTTTCAA GGACTTCCTC 
GTCATGGGTC TGAAGAACTT CCAGGAGTAC CAGGTCTGCT GCGCAGCTGT TGGTCTCACC 
GGCGACATTT TCCGCGCCCT TAAGGACCTA ATGGTGCCCT ATTCCAACGA AATTATGACC 
GTATTGATAA ACAATCTAAC GGAGCCGACT ATCCATCGAA CCGTCAAGCC CCAGGTTCTG 
TCTGCTTTCG GAGATATTGC GTTGAGCATT GGCAACCACT TCCTGCCATA TTTGAGCATG 
GTCCTAGATA TGTTGCGCGT TGCGTCCAAT CTGCAGACGG ACGCCAACAA CTTCGATATG 
AACGAATACA TCAACGAGCT GCGTGAAAGC ATCCTTGAAG CCTACACTGG CATCATCCAG 
GGCCTTAAGG GCGTTGACCA GACTGCCCAT ACAGACGTTA TGCACATGGA GCCCCACCTG 
ATGCACATTA TTTCGTTCAT CAAGCGCATC GCTCAGGAGG GCGACGTGTC CGACTCAATG 
TTGGCCAGTG CCGCTGGCTT CATTGGCGAT CTGTGCACTT CGTTTGGTCC GCGGCTATAC 
CCGCTGCTGG ATGATGCCAT CATTACCCAA TTCCTTGCCG AGGGCAAGCG CTCCAAGGCG 
CAGCGCACCA AGATGCTGTG CACCTGGGCC GTCAAAGAGA TAAAAAAAAT CAATACCCAG 
GTCATCACGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fs(2)Ket ( NM_001259179.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246108.1 Drosophila melanogaster female sterile (2) ketel, transcript variant D (Fs(2)Ket), mRNA.

Price & Availability↑

CloneID	OFa15055
Clone ID Related Accession (Same CDS sequence)	NM_058148.5 , NM_001259182.2 , NM_001259179.2 , NM_001259180.2 , NM_001259181.2
Accession Version	NM_001259179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	female sterile (2) ketel, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259179.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTTCCG ATATCGCCAT GCAATTGATT GCCATTTTGG AAAAGACGGT GTCGCCAGAC 
AAAAATGAGC TGCTATCGGC GAAGAACTTT CTGGAGCAGG CGGCGGCCAG CAACCTGCCC 
GAGTTTCTCA AGGCGCTGTC AGAGATACTG GTGAACACGG CCAACAGTGC GGTGGCGCGA 
ATGGCAGCCG GTCTCCAGCT GAAGAACCAC CTGACCAGCA AGGACGAGAA GGTCAGCCAA 
CAGTACCAGG ATCGCTGGCA TCAGTTTCCC AGCGAGATCC GCGAGTTGAT CAAGAATAAC 
ATCCTGGCTG CTTTGGGTAC CGAGAACACC CGACCCTCCT GCGCCGCCCA GTGCGTGGCC 
TATGTGGCCG TGATTGAGCT GCCGATAAAC CGCTGGCCCA TGCTCATCCA GACACTGGTG 
AACAAGGTGG TCAGCGAAGG ATCCAGCGAG ATGCATCGCG AGTCGGCGCT GGAGGCCATT 
GGCTACATTT GCCAGGACAT TCGTTTCGGC GTCATGGAGA ACCAGTCGAA CGATGTGCTG 
ACTGCTATTA TTCACGGCAT GAGAAAGGTA GAGCCGAGCA ATCATGTACG CTTGGCGGCC 
ACCACGGCCC TTCACAACTC GTTGGAGTTC ACCAAATCGA ACTTTGAGAA AGACATGGAG 
CGCAACTTCA TCATGGAGGT GGTGTGCGAG GCCACTCAGT GCCAGGACTC GCAAATCTGC 
GTTGCCGCTC TGCAGTGCCT GGTGAAGATT ATGACCCTTT ATTACCAGTA CATGGAGCCC 
TACATGGCAC AGGCTCTTTT CCCTATTACT TTGGCGGCCA TGAAGTCTGA TAACGATGCT 
GTAGCGCTGC AGGGCATAGA GTTCTGGTCG AACGTCTGCG ATGAGGAGAT AGATCTGGCC 
ATCGAAAGCC AGGAGGCCAC TGATCAGGGC CGCGCTCCGC AGAGAGTGTC CAAGCACTAT 
GCTCGAGGTG CTCTGCAGTT CCTGACGCCT GTGCTCGTCG AGAAATTAAC CAAGCAGGAC 
GAGTGCGATG ACGAGGACAC CTGGAGCCCG GCGAAGGCCG CCTCCGTTTG CCTCATGGTG 
CTGGCCACAT GTTGCGAGGA CGAGATCGTG CCACATGTGC TTCCCTTCAT CAAGGAGAAC 
ATCGAGTCAC CTAATTGGCG ATTCCGCGAC GCAGCGGTAA TGACTTTCGG CTCCGTGCTG 
AATGGATTGG AGACCAATAC CTTAAAGCCG CTCGTGGAGC AAGCCATGCC CACGCTCATT 
CGACTGATGT ACGACTCCAG CGTTATCGTG CGCGATACCA TCGCCTGGAC CTTCGGACGT 
ATTTGCGATA TAATTCCCGA GGCGGCTATC AACGAGACAT ATCTTCAAAC GCTTTTGGAG 
TGCTTCGTGA AGAGCCTAAA GTCAGAGCCG CGTGTAGCAG CCAACGTGTG TTGGGCCTTC 
ATTGGACTTT CCGATGCAGC TTGGGAGGCA GCGGTCACCA ACGACGGAGA GACTCCGGAG 
ACGTACGCCC TGTCCCCGTA CTTTGAGTAC ATCATTACCC AGTTGCTGGA GACCACTGAT 
CGATCGGACG GAGCTCAAGC CAATTTGCGA TGTGCTGCTT ATCAGGCACT TATGGATATG 
ATCAAGAACT CTCCCTTGGA TTGCTACTTG GTGGTCCAGC GCACCACGCT GGTGATTTTG 
GAGCGCTTGA ACCAGGTGAT GCAGATGGAA ACGCAGATTA ACAATCACAG CGATCGTCAT 
CAGTTCAACG ATCTACAATC GCTTCTGTGC GCCACTCTGC AAAGCGTTTT GAGAAAGGTC 
CACGAGCAGG ATGCGCCTCA AATTTCCGAT GCCATTATGA CCGCTCTGCT GACCATGTTC 
AATTCAAGTG CTGGAAAGTC GGGTGTAGTG CAGGAGGAGG CCTTCCTTGC CGTTTCGACA 
TTGGTTGAGC TTTTGGGAGC TCAGTTCGCC AAGTACATGC CCGCTTTCAA GGACTTCCTC 
GTCATGGGTC TGAAGAACTT CCAGGAGTAC CAGGTCTGCT GCGCAGCTGT TGGTCTCACC 
GGCGACATTT TCCGCGCCCT TAAGGACCTA ATGGTGCCCT ATTCCAACGA AATTATGACC 
GTATTGATAA ACAATCTAAC GGAGCCGACT ATCCATCGAA CCGTCAAGCC CCAGGTTCTG 
TCTGCTTTCG GAGATATTGC GTTGAGCATT GGCAACCACT TCCTGCCATA TTTGAGCATG 
GTCCTAGATA TGTTGCGCGT TGCGTCCAAT CTGCAGACGG ACGCCAACAA CTTCGATATG 
AACGAATACA TCAACGAGCT GCGTGAAAGC ATCCTTGAAG CCTACACTGG CATCATCCAG 
GGCCTTAAGG GCGTTGACCA GACTGCCCAT ACAGACGTTA TGCACATGGA GCCCCACCTG 
ATGCACATTA TTTCGTTCAT CAAGCGCATC GCTCAGGAGG GCGACGTGTC CGACTCAATG 
TTGGCCAGTG CCGCTGGCTT CATTGGCGAT CTGTGCACTT CGTTTGGTCC GCGGCTATAC 
CCGCTGCTGG ATGATGCCAT CATTACCCAA TTCCTTGCCG AGGGCAAGCG CTCCAAGGCG 
CAGCGCACCA AGATGCTGTG CACCTGGGCC GTCAAAGAGA TAAAAAAAAT CAATACCCAG 
GTCATCACGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246108.1
CDS	301..2955
Translation	MTSDIAMQLIAILEKTVSPDKNELLSAKNFLEQAAASNLPEFLKALSEILVNTANSAVARMAAGLQLKNHLTSKDEKVSQQYQDRWHQFPSEIRELIKNNILAALGTENTRPSCAAQCVAYVAVIELPINRWPMLIQTLVNKVVSEGSSEMHRESALEAIGYICQDIRFGVMENQSNDVLTAIIHGMRKVEPSNHVRLAATTALHNSLEFTKSNFEKDMERNFIMEVVCEATQCQDSQICVAALQCLVKIMTLYYQYMEPYMAQALFPITLAAMKSDNDAVALQGIEFWSNVCDEEIDLAIESQEATDQGRAPQRVSKHYARGALQFLTPVLVEKLTKQDECDDEDTWSPAKAASVCLMVLATCCEDEIVPHVLPFIKENIESPNWRFRDAAVMTFGSVLNGLETNTLKPLVEQAMPTLIRLMYDSSVIVRDTIAWTFGRICDIIPEAAINETYLQTLLECFVKSLKSEPRVAANVCWAFIGLSDAAWEAAVTNDGETPETYALSPYFEYIITQLLETTDRSDGAQANLRCAAYQALMDMIKNSPLDCYLVVQRTTLVILERLNQVMQMETQINNHSDRHQFNDLQSLLCATLQSVLRKVHEQDAPQISDAIMTALLTMFNSSAGKSGVVQEEAFLAVSTLVELLGAQFAKYMPAFKDFLVMGLKNFQEYQVCCAAVGLTGDIFRALKDLMVPYSNEIMTVLINNLTEPTIHRTVKPQVLSAFGDIALSIGNHFLPYLSMVLDMLRVASNLQTDANNFDMNEYINELRESILEAYTGIIQGLKGVDQTAHTDVMHMEPHLMHIISFIKRIAQEGDVSDSMLASAAGFIGDLCTSFGPRLYPLLDDAIITQFLAEGKRSKAQRTKMLCTWAVKEIKKINTQVITQ

Target ORF information:

RefSeq Version	NM_001259179.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster female sterile (2) ketel, transcript variant D (Fs(2)Ket), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259179.2

ORF Insert Sequence:

ATGACTTCCG ATATCGCCAT GCAATTGATT GCCATTTTGG AAAAGACGGT GTCGCCAGAC 
AAAAATGAGC TGCTATCGGC GAAGAACTTT CTGGAGCAGG CGGCGGCCAG CAACCTGCCC 
GAGTTTCTCA AGGCGCTGTC AGAGATACTG GTGAACACGG CCAACAGTGC GGTGGCGCGA 
ATGGCAGCCG GTCTCCAGCT GAAGAACCAC CTGACCAGCA AGGACGAGAA GGTCAGCCAA 
CAGTACCAGG ATCGCTGGCA TCAGTTTCCC AGCGAGATCC GCGAGTTGAT CAAGAATAAC 
ATCCTGGCTG CTTTGGGTAC CGAGAACACC CGACCCTCCT GCGCCGCCCA GTGCGTGGCC 
TATGTGGCCG TGATTGAGCT GCCGATAAAC CGCTGGCCCA TGCTCATCCA GACACTGGTG 
AACAAGGTGG TCAGCGAAGG ATCCAGCGAG ATGCATCGCG AGTCGGCGCT GGAGGCCATT 
GGCTACATTT GCCAGGACAT TCGTTTCGGC GTCATGGAGA ACCAGTCGAA CGATGTGCTG 
ACTGCTATTA TTCACGGCAT GAGAAAGGTA GAGCCGAGCA ATCATGTACG CTTGGCGGCC 
ACCACGGCCC TTCACAACTC GTTGGAGTTC ACCAAATCGA ACTTTGAGAA AGACATGGAG 
CGCAACTTCA TCATGGAGGT GGTGTGCGAG GCCACTCAGT GCCAGGACTC GCAAATCTGC 
GTTGCCGCTC TGCAGTGCCT GGTGAAGATT ATGACCCTTT ATTACCAGTA CATGGAGCCC 
TACATGGCAC AGGCTCTTTT CCCTATTACT TTGGCGGCCA TGAAGTCTGA TAACGATGCT 
GTAGCGCTGC AGGGCATAGA GTTCTGGTCG AACGTCTGCG ATGAGGAGAT AGATCTGGCC 
ATCGAAAGCC AGGAGGCCAC TGATCAGGGC CGCGCTCCGC AGAGAGTGTC CAAGCACTAT 
GCTCGAGGTG CTCTGCAGTT CCTGACGCCT GTGCTCGTCG AGAAATTAAC CAAGCAGGAC 
GAGTGCGATG ACGAGGACAC CTGGAGCCCG GCGAAGGCCG CCTCCGTTTG CCTCATGGTG 
CTGGCCACAT GTTGCGAGGA CGAGATCGTG CCACATGTGC TTCCCTTCAT CAAGGAGAAC 
ATCGAGTCAC CTAATTGGCG ATTCCGCGAC GCAGCGGTAA TGACTTTCGG CTCCGTGCTG 
AATGGATTGG AGACCAATAC CTTAAAGCCG CTCGTGGAGC AAGCCATGCC CACGCTCATT 
CGACTGATGT ACGACTCCAG CGTTATCGTG CGCGATACCA TCGCCTGGAC CTTCGGACGT 
ATTTGCGATA TAATTCCCGA GGCGGCTATC AACGAGACAT ATCTTCAAAC GCTTTTGGAG 
TGCTTCGTGA AGAGCCTAAA GTCAGAGCCG CGTGTAGCAG CCAACGTGTG TTGGGCCTTC 
ATTGGACTTT CCGATGCAGC TTGGGAGGCA GCGGTCACCA ACGACGGAGA GACTCCGGAG 
ACGTACGCCC TGTCCCCGTA CTTTGAGTAC ATCATTACCC AGTTGCTGGA GACCACTGAT 
CGATCGGACG GAGCTCAAGC CAATTTGCGA TGTGCTGCTT ATCAGGCACT TATGGATATG 
ATCAAGAACT CTCCCTTGGA TTGCTACTTG GTGGTCCAGC GCACCACGCT GGTGATTTTG 
GAGCGCTTGA ACCAGGTGAT GCAGATGGAA ACGCAGATTA ACAATCACAG CGATCGTCAT 
CAGTTCAACG ATCTACAATC GCTTCTGTGC GCCACTCTGC AAAGCGTTTT GAGAAAGGTC 
CACGAGCAGG ATGCGCCTCA AATTTCCGAT GCCATTATGA CCGCTCTGCT GACCATGTTC 
AATTCAAGTG CTGGAAAGTC GGGTGTAGTG CAGGAGGAGG CCTTCCTTGC CGTTTCGACA 
TTGGTTGAGC TTTTGGGAGC TCAGTTCGCC AAGTACATGC CCGCTTTCAA GGACTTCCTC 
GTCATGGGTC TGAAGAACTT CCAGGAGTAC CAGGTCTGCT GCGCAGCTGT TGGTCTCACC 
GGCGACATTT TCCGCGCCCT TAAGGACCTA ATGGTGCCCT ATTCCAACGA AATTATGACC 
GTATTGATAA ACAATCTAAC GGAGCCGACT ATCCATCGAA CCGTCAAGCC CCAGGTTCTG 
TCTGCTTTCG GAGATATTGC GTTGAGCATT GGCAACCACT TCCTGCCATA TTTGAGCATG 
GTCCTAGATA TGTTGCGCGT TGCGTCCAAT CTGCAGACGG ACGCCAACAA CTTCGATATG 
AACGAATACA TCAACGAGCT GCGTGAAAGC ATCCTTGAAG CCTACACTGG CATCATCCAG 
GGCCTTAAGG GCGTTGACCA GACTGCCCAT ACAGACGTTA TGCACATGGA GCCCCACCTG 
ATGCACATTA TTTCGTTCAT CAAGCGCATC GCTCAGGAGG GCGACGTGTC CGACTCAATG 
TTGGCCAGTG CCGCTGGCTT CATTGGCGAT CTGTGCACTT CGTTTGGTCC GCGGCTATAC 
CCGCTGCTGG ATGATGCCAT CATTACCCAA TTCCTTGCCG AGGGCAAGCG CTCCAAGGCG 
CAGCGCACCA AGATGCTGTG CACCTGGGCC GTCAAAGAGA TAAAAAAAAT CAATACCCAG 
GTCATCACGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fs(2)Ket ( NM_001259180.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246109.1 Drosophila melanogaster female sterile (2) ketel, transcript variant B (Fs(2)Ket), mRNA.

Price & Availability↑

CloneID	OFa15055
Clone ID Related Accession (Same CDS sequence)	NM_058148.5 , NM_001259182.2 , NM_001259179.2 , NM_001259180.2 , NM_001259181.2
Accession Version	NM_001259180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	female sterile (2) ketel, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259180.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTTCCG ATATCGCCAT GCAATTGATT GCCATTTTGG AAAAGACGGT GTCGCCAGAC 
AAAAATGAGC TGCTATCGGC GAAGAACTTT CTGGAGCAGG CGGCGGCCAG CAACCTGCCC 
GAGTTTCTCA AGGCGCTGTC AGAGATACTG GTGAACACGG CCAACAGTGC GGTGGCGCGA 
ATGGCAGCCG GTCTCCAGCT GAAGAACCAC CTGACCAGCA AGGACGAGAA GGTCAGCCAA 
CAGTACCAGG ATCGCTGGCA TCAGTTTCCC AGCGAGATCC GCGAGTTGAT CAAGAATAAC 
ATCCTGGCTG CTTTGGGTAC CGAGAACACC CGACCCTCCT GCGCCGCCCA GTGCGTGGCC 
TATGTGGCCG TGATTGAGCT GCCGATAAAC CGCTGGCCCA TGCTCATCCA GACACTGGTG 
AACAAGGTGG TCAGCGAAGG ATCCAGCGAG ATGCATCGCG AGTCGGCGCT GGAGGCCATT 
GGCTACATTT GCCAGGACAT TCGTTTCGGC GTCATGGAGA ACCAGTCGAA CGATGTGCTG 
ACTGCTATTA TTCACGGCAT GAGAAAGGTA GAGCCGAGCA ATCATGTACG CTTGGCGGCC 
ACCACGGCCC TTCACAACTC GTTGGAGTTC ACCAAATCGA ACTTTGAGAA AGACATGGAG 
CGCAACTTCA TCATGGAGGT GGTGTGCGAG GCCACTCAGT GCCAGGACTC GCAAATCTGC 
GTTGCCGCTC TGCAGTGCCT GGTGAAGATT ATGACCCTTT ATTACCAGTA CATGGAGCCC 
TACATGGCAC AGGCTCTTTT CCCTATTACT TTGGCGGCCA TGAAGTCTGA TAACGATGCT 
GTAGCGCTGC AGGGCATAGA GTTCTGGTCG AACGTCTGCG ATGAGGAGAT AGATCTGGCC 
ATCGAAAGCC AGGAGGCCAC TGATCAGGGC CGCGCTCCGC AGAGAGTGTC CAAGCACTAT 
GCTCGAGGTG CTCTGCAGTT CCTGACGCCT GTGCTCGTCG AGAAATTAAC CAAGCAGGAC 
GAGTGCGATG ACGAGGACAC CTGGAGCCCG GCGAAGGCCG CCTCCGTTTG CCTCATGGTG 
CTGGCCACAT GTTGCGAGGA CGAGATCGTG CCACATGTGC TTCCCTTCAT CAAGGAGAAC 
ATCGAGTCAC CTAATTGGCG ATTCCGCGAC GCAGCGGTAA TGACTTTCGG CTCCGTGCTG 
AATGGATTGG AGACCAATAC CTTAAAGCCG CTCGTGGAGC AAGCCATGCC CACGCTCATT 
CGACTGATGT ACGACTCCAG CGTTATCGTG CGCGATACCA TCGCCTGGAC CTTCGGACGT 
ATTTGCGATA TAATTCCCGA GGCGGCTATC AACGAGACAT ATCTTCAAAC GCTTTTGGAG 
TGCTTCGTGA AGAGCCTAAA GTCAGAGCCG CGTGTAGCAG CCAACGTGTG TTGGGCCTTC 
ATTGGACTTT CCGATGCAGC TTGGGAGGCA GCGGTCACCA ACGACGGAGA GACTCCGGAG 
ACGTACGCCC TGTCCCCGTA CTTTGAGTAC ATCATTACCC AGTTGCTGGA GACCACTGAT 
CGATCGGACG GAGCTCAAGC CAATTTGCGA TGTGCTGCTT ATCAGGCACT TATGGATATG 
ATCAAGAACT CTCCCTTGGA TTGCTACTTG GTGGTCCAGC GCACCACGCT GGTGATTTTG 
GAGCGCTTGA ACCAGGTGAT GCAGATGGAA ACGCAGATTA ACAATCACAG CGATCGTCAT 
CAGTTCAACG ATCTACAATC GCTTCTGTGC GCCACTCTGC AAAGCGTTTT GAGAAAGGTC 
CACGAGCAGG ATGCGCCTCA AATTTCCGAT GCCATTATGA CCGCTCTGCT GACCATGTTC 
AATTCAAGTG CTGGAAAGTC GGGTGTAGTG CAGGAGGAGG CCTTCCTTGC CGTTTCGACA 
TTGGTTGAGC TTTTGGGAGC TCAGTTCGCC AAGTACATGC CCGCTTTCAA GGACTTCCTC 
GTCATGGGTC TGAAGAACTT CCAGGAGTAC CAGGTCTGCT GCGCAGCTGT TGGTCTCACC 
GGCGACATTT TCCGCGCCCT TAAGGACCTA ATGGTGCCCT ATTCCAACGA AATTATGACC 
GTATTGATAA ACAATCTAAC GGAGCCGACT ATCCATCGAA CCGTCAAGCC CCAGGTTCTG 
TCTGCTTTCG GAGATATTGC GTTGAGCATT GGCAACCACT TCCTGCCATA TTTGAGCATG 
GTCCTAGATA TGTTGCGCGT TGCGTCCAAT CTGCAGACGG ACGCCAACAA CTTCGATATG 
AACGAATACA TCAACGAGCT GCGTGAAAGC ATCCTTGAAG CCTACACTGG CATCATCCAG 
GGCCTTAAGG GCGTTGACCA GACTGCCCAT ACAGACGTTA TGCACATGGA GCCCCACCTG 
ATGCACATTA TTTCGTTCAT CAAGCGCATC GCTCAGGAGG GCGACGTGTC CGACTCAATG 
TTGGCCAGTG CCGCTGGCTT CATTGGCGAT CTGTGCACTT CGTTTGGTCC GCGGCTATAC 
CCGCTGCTGG ATGATGCCAT CATTACCCAA TTCCTTGCCG AGGGCAAGCG CTCCAAGGCG 
CAGCGCACCA AGATGCTGTG CACCTGGGCC GTCAAAGAGA TAAAAAAAAT CAATACCCAG 
GTCATCACGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246109.1
CDS	483..3137
Translation	MTSDIAMQLIAILEKTVSPDKNELLSAKNFLEQAAASNLPEFLKALSEILVNTANSAVARMAAGLQLKNHLTSKDEKVSQQYQDRWHQFPSEIRELIKNNILAALGTENTRPSCAAQCVAYVAVIELPINRWPMLIQTLVNKVVSEGSSEMHRESALEAIGYICQDIRFGVMENQSNDVLTAIIHGMRKVEPSNHVRLAATTALHNSLEFTKSNFEKDMERNFIMEVVCEATQCQDSQICVAALQCLVKIMTLYYQYMEPYMAQALFPITLAAMKSDNDAVALQGIEFWSNVCDEEIDLAIESQEATDQGRAPQRVSKHYARGALQFLTPVLVEKLTKQDECDDEDTWSPAKAASVCLMVLATCCEDEIVPHVLPFIKENIESPNWRFRDAAVMTFGSVLNGLETNTLKPLVEQAMPTLIRLMYDSSVIVRDTIAWTFGRICDIIPEAAINETYLQTLLECFVKSLKSEPRVAANVCWAFIGLSDAAWEAAVTNDGETPETYALSPYFEYIITQLLETTDRSDGAQANLRCAAYQALMDMIKNSPLDCYLVVQRTTLVILERLNQVMQMETQINNHSDRHQFNDLQSLLCATLQSVLRKVHEQDAPQISDAIMTALLTMFNSSAGKSGVVQEEAFLAVSTLVELLGAQFAKYMPAFKDFLVMGLKNFQEYQVCCAAVGLTGDIFRALKDLMVPYSNEIMTVLINNLTEPTIHRTVKPQVLSAFGDIALSIGNHFLPYLSMVLDMLRVASNLQTDANNFDMNEYINELRESILEAYTGIIQGLKGVDQTAHTDVMHMEPHLMHIISFIKRIAQEGDVSDSMLASAAGFIGDLCTSFGPRLYPLLDDAIITQFLAEGKRSKAQRTKMLCTWAVKEIKKINTQVITQ

Target ORF information:

RefSeq Version	NM_001259180.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster female sterile (2) ketel, transcript variant B (Fs(2)Ket), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259180.2

ORF Insert Sequence:

ATGACTTCCG ATATCGCCAT GCAATTGATT GCCATTTTGG AAAAGACGGT GTCGCCAGAC 
AAAAATGAGC TGCTATCGGC GAAGAACTTT CTGGAGCAGG CGGCGGCCAG CAACCTGCCC 
GAGTTTCTCA AGGCGCTGTC AGAGATACTG GTGAACACGG CCAACAGTGC GGTGGCGCGA 
ATGGCAGCCG GTCTCCAGCT GAAGAACCAC CTGACCAGCA AGGACGAGAA GGTCAGCCAA 
CAGTACCAGG ATCGCTGGCA TCAGTTTCCC AGCGAGATCC GCGAGTTGAT CAAGAATAAC 
ATCCTGGCTG CTTTGGGTAC CGAGAACACC CGACCCTCCT GCGCCGCCCA GTGCGTGGCC 
TATGTGGCCG TGATTGAGCT GCCGATAAAC CGCTGGCCCA TGCTCATCCA GACACTGGTG 
AACAAGGTGG TCAGCGAAGG ATCCAGCGAG ATGCATCGCG AGTCGGCGCT GGAGGCCATT 
GGCTACATTT GCCAGGACAT TCGTTTCGGC GTCATGGAGA ACCAGTCGAA CGATGTGCTG 
ACTGCTATTA TTCACGGCAT GAGAAAGGTA GAGCCGAGCA ATCATGTACG CTTGGCGGCC 
ACCACGGCCC TTCACAACTC GTTGGAGTTC ACCAAATCGA ACTTTGAGAA AGACATGGAG 
CGCAACTTCA TCATGGAGGT GGTGTGCGAG GCCACTCAGT GCCAGGACTC GCAAATCTGC 
GTTGCCGCTC TGCAGTGCCT GGTGAAGATT ATGACCCTTT ATTACCAGTA CATGGAGCCC 
TACATGGCAC AGGCTCTTTT CCCTATTACT TTGGCGGCCA TGAAGTCTGA TAACGATGCT 
GTAGCGCTGC AGGGCATAGA GTTCTGGTCG AACGTCTGCG ATGAGGAGAT AGATCTGGCC 
ATCGAAAGCC AGGAGGCCAC TGATCAGGGC CGCGCTCCGC AGAGAGTGTC CAAGCACTAT 
GCTCGAGGTG CTCTGCAGTT CCTGACGCCT GTGCTCGTCG AGAAATTAAC CAAGCAGGAC 
GAGTGCGATG ACGAGGACAC CTGGAGCCCG GCGAAGGCCG CCTCCGTTTG CCTCATGGTG 
CTGGCCACAT GTTGCGAGGA CGAGATCGTG CCACATGTGC TTCCCTTCAT CAAGGAGAAC 
ATCGAGTCAC CTAATTGGCG ATTCCGCGAC GCAGCGGTAA TGACTTTCGG CTCCGTGCTG 
AATGGATTGG AGACCAATAC CTTAAAGCCG CTCGTGGAGC AAGCCATGCC CACGCTCATT 
CGACTGATGT ACGACTCCAG CGTTATCGTG CGCGATACCA TCGCCTGGAC CTTCGGACGT 
ATTTGCGATA TAATTCCCGA GGCGGCTATC AACGAGACAT ATCTTCAAAC GCTTTTGGAG 
TGCTTCGTGA AGAGCCTAAA GTCAGAGCCG CGTGTAGCAG CCAACGTGTG TTGGGCCTTC 
ATTGGACTTT CCGATGCAGC TTGGGAGGCA GCGGTCACCA ACGACGGAGA GACTCCGGAG 
ACGTACGCCC TGTCCCCGTA CTTTGAGTAC ATCATTACCC AGTTGCTGGA GACCACTGAT 
CGATCGGACG GAGCTCAAGC CAATTTGCGA TGTGCTGCTT ATCAGGCACT TATGGATATG 
ATCAAGAACT CTCCCTTGGA TTGCTACTTG GTGGTCCAGC GCACCACGCT GGTGATTTTG 
GAGCGCTTGA ACCAGGTGAT GCAGATGGAA ACGCAGATTA ACAATCACAG CGATCGTCAT 
CAGTTCAACG ATCTACAATC GCTTCTGTGC GCCACTCTGC AAAGCGTTTT GAGAAAGGTC 
CACGAGCAGG ATGCGCCTCA AATTTCCGAT GCCATTATGA CCGCTCTGCT GACCATGTTC 
AATTCAAGTG CTGGAAAGTC GGGTGTAGTG CAGGAGGAGG CCTTCCTTGC CGTTTCGACA 
TTGGTTGAGC TTTTGGGAGC TCAGTTCGCC AAGTACATGC CCGCTTTCAA GGACTTCCTC 
GTCATGGGTC TGAAGAACTT CCAGGAGTAC CAGGTCTGCT GCGCAGCTGT TGGTCTCACC 
GGCGACATTT TCCGCGCCCT TAAGGACCTA ATGGTGCCCT ATTCCAACGA AATTATGACC 
GTATTGATAA ACAATCTAAC GGAGCCGACT ATCCATCGAA CCGTCAAGCC CCAGGTTCTG 
TCTGCTTTCG GAGATATTGC GTTGAGCATT GGCAACCACT TCCTGCCATA TTTGAGCATG 
GTCCTAGATA TGTTGCGCGT TGCGTCCAAT CTGCAGACGG ACGCCAACAA CTTCGATATG 
AACGAATACA TCAACGAGCT GCGTGAAAGC ATCCTTGAAG CCTACACTGG CATCATCCAG 
GGCCTTAAGG GCGTTGACCA GACTGCCCAT ACAGACGTTA TGCACATGGA GCCCCACCTG 
ATGCACATTA TTTCGTTCAT CAAGCGCATC GCTCAGGAGG GCGACGTGTC CGACTCAATG 
TTGGCCAGTG CCGCTGGCTT CATTGGCGAT CTGTGCACTT CGTTTGGTCC GCGGCTATAC 
CCGCTGCTGG ATGATGCCAT CATTACCCAA TTCCTTGCCG AGGGCAAGCG CTCCAAGGCG 
CAGCGCACCA AGATGCTGTG CACCTGGGCC GTCAAAGAGA TAAAAAAAAT CAATACCCAG 
GTCATCACGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fs(2)Ket ( NM_001259181.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246110.1 Drosophila melanogaster female sterile (2) ketel, transcript variant C (Fs(2)Ket), mRNA.

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CloneID	OFa15055
Clone ID Related Accession (Same CDS sequence)	NM_058148.5 , NM_001259182.2 , NM_001259179.2 , NM_001259180.2 , NM_001259181.2
Accession Version	NM_001259181.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	female sterile (2) ketel, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259181.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTTCCG ATATCGCCAT GCAATTGATT GCCATTTTGG AAAAGACGGT GTCGCCAGAC 
AAAAATGAGC TGCTATCGGC GAAGAACTTT CTGGAGCAGG CGGCGGCCAG CAACCTGCCC 
GAGTTTCTCA AGGCGCTGTC AGAGATACTG GTGAACACGG CCAACAGTGC GGTGGCGCGA 
ATGGCAGCCG GTCTCCAGCT GAAGAACCAC CTGACCAGCA AGGACGAGAA GGTCAGCCAA 
CAGTACCAGG ATCGCTGGCA TCAGTTTCCC AGCGAGATCC GCGAGTTGAT CAAGAATAAC 
ATCCTGGCTG CTTTGGGTAC CGAGAACACC CGACCCTCCT GCGCCGCCCA GTGCGTGGCC 
TATGTGGCCG TGATTGAGCT GCCGATAAAC CGCTGGCCCA TGCTCATCCA GACACTGGTG 
AACAAGGTGG TCAGCGAAGG ATCCAGCGAG ATGCATCGCG AGTCGGCGCT GGAGGCCATT 
GGCTACATTT GCCAGGACAT TCGTTTCGGC GTCATGGAGA ACCAGTCGAA CGATGTGCTG 
ACTGCTATTA TTCACGGCAT GAGAAAGGTA GAGCCGAGCA ATCATGTACG CTTGGCGGCC 
ACCACGGCCC TTCACAACTC GTTGGAGTTC ACCAAATCGA ACTTTGAGAA AGACATGGAG 
CGCAACTTCA TCATGGAGGT GGTGTGCGAG GCCACTCAGT GCCAGGACTC GCAAATCTGC 
GTTGCCGCTC TGCAGTGCCT GGTGAAGATT ATGACCCTTT ATTACCAGTA CATGGAGCCC 
TACATGGCAC AGGCTCTTTT CCCTATTACT TTGGCGGCCA TGAAGTCTGA TAACGATGCT 
GTAGCGCTGC AGGGCATAGA GTTCTGGTCG AACGTCTGCG ATGAGGAGAT AGATCTGGCC 
ATCGAAAGCC AGGAGGCCAC TGATCAGGGC CGCGCTCCGC AGAGAGTGTC CAAGCACTAT 
GCTCGAGGTG CTCTGCAGTT CCTGACGCCT GTGCTCGTCG AGAAATTAAC CAAGCAGGAC 
GAGTGCGATG ACGAGGACAC CTGGAGCCCG GCGAAGGCCG CCTCCGTTTG CCTCATGGTG 
CTGGCCACAT GTTGCGAGGA CGAGATCGTG CCACATGTGC TTCCCTTCAT CAAGGAGAAC 
ATCGAGTCAC CTAATTGGCG ATTCCGCGAC GCAGCGGTAA TGACTTTCGG CTCCGTGCTG 
AATGGATTGG AGACCAATAC CTTAAAGCCG CTCGTGGAGC AAGCCATGCC CACGCTCATT 
CGACTGATGT ACGACTCCAG CGTTATCGTG CGCGATACCA TCGCCTGGAC CTTCGGACGT 
ATTTGCGATA TAATTCCCGA GGCGGCTATC AACGAGACAT ATCTTCAAAC GCTTTTGGAG 
TGCTTCGTGA AGAGCCTAAA GTCAGAGCCG CGTGTAGCAG CCAACGTGTG TTGGGCCTTC 
ATTGGACTTT CCGATGCAGC TTGGGAGGCA GCGGTCACCA ACGACGGAGA GACTCCGGAG 
ACGTACGCCC TGTCCCCGTA CTTTGAGTAC ATCATTACCC AGTTGCTGGA GACCACTGAT 
CGATCGGACG GAGCTCAAGC CAATTTGCGA TGTGCTGCTT ATCAGGCACT TATGGATATG 
ATCAAGAACT CTCCCTTGGA TTGCTACTTG GTGGTCCAGC GCACCACGCT GGTGATTTTG 
GAGCGCTTGA ACCAGGTGAT GCAGATGGAA ACGCAGATTA ACAATCACAG CGATCGTCAT 
CAGTTCAACG ATCTACAATC GCTTCTGTGC GCCACTCTGC AAAGCGTTTT GAGAAAGGTC 
CACGAGCAGG ATGCGCCTCA AATTTCCGAT GCCATTATGA CCGCTCTGCT GACCATGTTC 
AATTCAAGTG CTGGAAAGTC GGGTGTAGTG CAGGAGGAGG CCTTCCTTGC CGTTTCGACA 
TTGGTTGAGC TTTTGGGAGC TCAGTTCGCC AAGTACATGC CCGCTTTCAA GGACTTCCTC 
GTCATGGGTC TGAAGAACTT CCAGGAGTAC CAGGTCTGCT GCGCAGCTGT TGGTCTCACC 
GGCGACATTT TCCGCGCCCT TAAGGACCTA ATGGTGCCCT ATTCCAACGA AATTATGACC 
GTATTGATAA ACAATCTAAC GGAGCCGACT ATCCATCGAA CCGTCAAGCC CCAGGTTCTG 
TCTGCTTTCG GAGATATTGC GTTGAGCATT GGCAACCACT TCCTGCCATA TTTGAGCATG 
GTCCTAGATA TGTTGCGCGT TGCGTCCAAT CTGCAGACGG ACGCCAACAA CTTCGATATG 
AACGAATACA TCAACGAGCT GCGTGAAAGC ATCCTTGAAG CCTACACTGG CATCATCCAG 
GGCCTTAAGG GCGTTGACCA GACTGCCCAT ACAGACGTTA TGCACATGGA GCCCCACCTG 
ATGCACATTA TTTCGTTCAT CAAGCGCATC GCTCAGGAGG GCGACGTGTC CGACTCAATG 
TTGGCCAGTG CCGCTGGCTT CATTGGCGAT CTGTGCACTT CGTTTGGTCC GCGGCTATAC 
CCGCTGCTGG ATGATGCCAT CATTACCCAA TTCCTTGCCG AGGGCAAGCG CTCCAAGGCG 
CAGCGCACCA AGATGCTGTG CACCTGGGCC GTCAAAGAGA TAAAAAAAAT CAATACCCAG 
GTCATCACGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246110.1
CDS	301..2955
Translation	MTSDIAMQLIAILEKTVSPDKNELLSAKNFLEQAAASNLPEFLKALSEILVNTANSAVARMAAGLQLKNHLTSKDEKVSQQYQDRWHQFPSEIRELIKNNILAALGTENTRPSCAAQCVAYVAVIELPINRWPMLIQTLVNKVVSEGSSEMHRESALEAIGYICQDIRFGVMENQSNDVLTAIIHGMRKVEPSNHVRLAATTALHNSLEFTKSNFEKDMERNFIMEVVCEATQCQDSQICVAALQCLVKIMTLYYQYMEPYMAQALFPITLAAMKSDNDAVALQGIEFWSNVCDEEIDLAIESQEATDQGRAPQRVSKHYARGALQFLTPVLVEKLTKQDECDDEDTWSPAKAASVCLMVLATCCEDEIVPHVLPFIKENIESPNWRFRDAAVMTFGSVLNGLETNTLKPLVEQAMPTLIRLMYDSSVIVRDTIAWTFGRICDIIPEAAINETYLQTLLECFVKSLKSEPRVAANVCWAFIGLSDAAWEAAVTNDGETPETYALSPYFEYIITQLLETTDRSDGAQANLRCAAYQALMDMIKNSPLDCYLVVQRTTLVILERLNQVMQMETQINNHSDRHQFNDLQSLLCATLQSVLRKVHEQDAPQISDAIMTALLTMFNSSAGKSGVVQEEAFLAVSTLVELLGAQFAKYMPAFKDFLVMGLKNFQEYQVCCAAVGLTGDIFRALKDLMVPYSNEIMTVLINNLTEPTIHRTVKPQVLSAFGDIALSIGNHFLPYLSMVLDMLRVASNLQTDANNFDMNEYINELRESILEAYTGIIQGLKGVDQTAHTDVMHMEPHLMHIISFIKRIAQEGDVSDSMLASAAGFIGDLCTSFGPRLYPLLDDAIITQFLAEGKRSKAQRTKMLCTWAVKEIKKINTQVITQ

Target ORF information:

RefSeq Version	NM_001259181.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster female sterile (2) ketel, transcript variant C (Fs(2)Ket), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259181.2

ORF Insert Sequence:

ATGACTTCCG ATATCGCCAT GCAATTGATT GCCATTTTGG AAAAGACGGT GTCGCCAGAC 
AAAAATGAGC TGCTATCGGC GAAGAACTTT CTGGAGCAGG CGGCGGCCAG CAACCTGCCC 
GAGTTTCTCA AGGCGCTGTC AGAGATACTG GTGAACACGG CCAACAGTGC GGTGGCGCGA 
ATGGCAGCCG GTCTCCAGCT GAAGAACCAC CTGACCAGCA AGGACGAGAA GGTCAGCCAA 
CAGTACCAGG ATCGCTGGCA TCAGTTTCCC AGCGAGATCC GCGAGTTGAT CAAGAATAAC 
ATCCTGGCTG CTTTGGGTAC CGAGAACACC CGACCCTCCT GCGCCGCCCA GTGCGTGGCC 
TATGTGGCCG TGATTGAGCT GCCGATAAAC CGCTGGCCCA TGCTCATCCA GACACTGGTG 
AACAAGGTGG TCAGCGAAGG ATCCAGCGAG ATGCATCGCG AGTCGGCGCT GGAGGCCATT 
GGCTACATTT GCCAGGACAT TCGTTTCGGC GTCATGGAGA ACCAGTCGAA CGATGTGCTG 
ACTGCTATTA TTCACGGCAT GAGAAAGGTA GAGCCGAGCA ATCATGTACG CTTGGCGGCC 
ACCACGGCCC TTCACAACTC GTTGGAGTTC ACCAAATCGA ACTTTGAGAA AGACATGGAG 
CGCAACTTCA TCATGGAGGT GGTGTGCGAG GCCACTCAGT GCCAGGACTC GCAAATCTGC 
GTTGCCGCTC TGCAGTGCCT GGTGAAGATT ATGACCCTTT ATTACCAGTA CATGGAGCCC 
TACATGGCAC AGGCTCTTTT CCCTATTACT TTGGCGGCCA TGAAGTCTGA TAACGATGCT 
GTAGCGCTGC AGGGCATAGA GTTCTGGTCG AACGTCTGCG ATGAGGAGAT AGATCTGGCC 
ATCGAAAGCC AGGAGGCCAC TGATCAGGGC CGCGCTCCGC AGAGAGTGTC CAAGCACTAT 
GCTCGAGGTG CTCTGCAGTT CCTGACGCCT GTGCTCGTCG AGAAATTAAC CAAGCAGGAC 
GAGTGCGATG ACGAGGACAC CTGGAGCCCG GCGAAGGCCG CCTCCGTTTG CCTCATGGTG 
CTGGCCACAT GTTGCGAGGA CGAGATCGTG CCACATGTGC TTCCCTTCAT CAAGGAGAAC 
ATCGAGTCAC CTAATTGGCG ATTCCGCGAC GCAGCGGTAA TGACTTTCGG CTCCGTGCTG 
AATGGATTGG AGACCAATAC CTTAAAGCCG CTCGTGGAGC AAGCCATGCC CACGCTCATT 
CGACTGATGT ACGACTCCAG CGTTATCGTG CGCGATACCA TCGCCTGGAC CTTCGGACGT 
ATTTGCGATA TAATTCCCGA GGCGGCTATC AACGAGACAT ATCTTCAAAC GCTTTTGGAG 
TGCTTCGTGA AGAGCCTAAA GTCAGAGCCG CGTGTAGCAG CCAACGTGTG TTGGGCCTTC 
ATTGGACTTT CCGATGCAGC TTGGGAGGCA GCGGTCACCA ACGACGGAGA GACTCCGGAG 
ACGTACGCCC TGTCCCCGTA CTTTGAGTAC ATCATTACCC AGTTGCTGGA GACCACTGAT 
CGATCGGACG GAGCTCAAGC CAATTTGCGA TGTGCTGCTT ATCAGGCACT TATGGATATG 
ATCAAGAACT CTCCCTTGGA TTGCTACTTG GTGGTCCAGC GCACCACGCT GGTGATTTTG 
GAGCGCTTGA ACCAGGTGAT GCAGATGGAA ACGCAGATTA ACAATCACAG CGATCGTCAT 
CAGTTCAACG ATCTACAATC GCTTCTGTGC GCCACTCTGC AAAGCGTTTT GAGAAAGGTC 
CACGAGCAGG ATGCGCCTCA AATTTCCGAT GCCATTATGA CCGCTCTGCT GACCATGTTC 
AATTCAAGTG CTGGAAAGTC GGGTGTAGTG CAGGAGGAGG CCTTCCTTGC CGTTTCGACA 
TTGGTTGAGC TTTTGGGAGC TCAGTTCGCC AAGTACATGC CCGCTTTCAA GGACTTCCTC 
GTCATGGGTC TGAAGAACTT CCAGGAGTAC CAGGTCTGCT GCGCAGCTGT TGGTCTCACC 
GGCGACATTT TCCGCGCCCT TAAGGACCTA ATGGTGCCCT ATTCCAACGA AATTATGACC 
GTATTGATAA ACAATCTAAC GGAGCCGACT ATCCATCGAA CCGTCAAGCC CCAGGTTCTG 
TCTGCTTTCG GAGATATTGC GTTGAGCATT GGCAACCACT TCCTGCCATA TTTGAGCATG 
GTCCTAGATA TGTTGCGCGT TGCGTCCAAT CTGCAGACGG ACGCCAACAA CTTCGATATG 
AACGAATACA TCAACGAGCT GCGTGAAAGC ATCCTTGAAG CCTACACTGG CATCATCCAG 
GGCCTTAAGG GCGTTGACCA GACTGCCCAT ACAGACGTTA TGCACATGGA GCCCCACCTG 
ATGCACATTA TTTCGTTCAT CAAGCGCATC GCTCAGGAGG GCGACGTGTC CGACTCAATG 
TTGGCCAGTG CCGCTGGCTT CATTGGCGAT CTGTGCACTT CGTTTGGTCC GCGGCTATAC 
CCGCTGCTGG ATGATGCCAT CATTACCCAA TTCCTTGCCG AGGGCAAGCG CTCCAAGGCG 
CAGCGCACCA AGATGCTGTG CACCTGGGCC GTCAAAGAGA TAAAAAAAAT CAATACCCAG 
GTCATCACGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Fs(2)Ket cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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