IKKepsilon cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	IKKepsilon
Entrez Gene ID	35329
Full Name	I-kappaB kinase epsilon
Synonyms	38D.31,APTX7,CG2615,DIK2,Dik2,DmIKKepsilon,DmIKKepsilon/dIK2,Dmel\CG2615,Dmik2,IK2,IKK,Ik2,Ik2/DmIKK,dIKK,dik2,ik2,l(2)38Ea
General protein information	Preferred Names I-kappaB kinase epsilon Names CG2615 gene product from transcript CG2615-RC CG2615-PB CG2615-PC IKK-related kinase IKKepsilon-PB IKKepsilon-PC IkappaB kinase-like 2 apoptoxin 7 ik2 lethal (2) 38Ea
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38D4-38D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_136204.3	NP_610048.2	I-kappaB kinase epsilon, isoform C
NM_165337.2	NP_724278.1	I-kappaB kinase epsilon, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TBK1	XP_538266.3
Mus musculus (house mouse)	Tbk1	NP_062760.3
Drosophila melanogaster (fruit fly)	ik2	NP_610048.2
Bos taurus (cattle)	TBK1	NP_001179684.1
Rattus norvegicus (Norway rat)	Tbk1	NP_001100256.1
Xenopus tropicalis (tropical clawed frog)	tbk1	NP_001135652.1
Pan troglodytes (chimpanzee)	TBK1	XP_509194.2
	TBK1	XP_001116924.1
Gallus gallus (chicken)	TBK1	NP_001186487.1
Danio rerio (zebrafish)	tbk1	NP_001038213.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009565	XP_318573.3
Homo sapiens (human)	TBK1	NP_037386.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings reveal a novel regulatory mechanism for dendrite pruning achieved by temporal activation of Ik2 kinase and dynein-mediated redistribution of Ik2/Spn-F complex in neurons
Title: Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons.

In glioma cell lines, the expression of IKKepsilon is suppressed by miR-98.
Title: Overexpression of miR-98 inhibits cell invasion in glioma cell lines via downregulation of IKK?.

These results suggest that polarized transport and selective retention regulate the distal tip localization of the Spn-F-IKKepsilon complex during bristle cell elongation.
Title: A transport and retention mechanism for the sustained distal localization of Spn-F-IKK? during Drosophila bristle elongation.

Spn-F mediates the interaction between Ik2 and Jvl to control Ik2 activity.
Title: Drosophila oocyte polarity and cytoskeleton organization require regulation of Ik2 activity by Spn-F and Javelin-like.

IKKepsilon: a kinase at the intersection of signaling and membrane traffic.
Title: IKKepsilon: a kinase at the intersection of signaling and membrane traffic.

The following IKKepsilon gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IKKepsilon cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15048	NM_136204.3 Latest version!	Drosophila melanogaster I-kappaB kinase epsilon (IKKepsilon), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15048	NM_165337.2 Latest version!	Drosophila melanogaster I-kappaB kinase epsilon (IKKepsilon), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IKKepsilon ( NM_136204.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610048.2 Drosophila melanogaster I-kappaB kinase epsilon (IKKepsilon), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa15048
Clone ID Related Accession (Same CDS sequence)	NM_136204.3 , NM_165337.2
Accession Version	NM_136204.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	I-kappaB kinase epsilon, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_136204.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCTTCC TGCGCGGTTC CGTGAGCTAT GTGTGGTGCA CCACCAGCGT CCTGGGAAAG 
GGAGCCACCG GTTCCGTGTT CCAGGGGGTC AACAAGATCA CCGGCGAATC GGTGGCGGTG 
AAGACCTTTA ATCCCTACAG TCACATGCGA CCGGCTGATG TGCAGATGCG GGAGTTCGAG 
GCCCTGAAAA AGGTCAACCA CGAGAATATA GTAAAGCTGT TGGCGATCGA GGAGGATCAA 
GAGGGGCGTG GTAAGGTGAT CGTGATGGAG CTCTGCACAG GCGGAAGTCT CTTTAACATC 
CTGGACGATC CTGAGAACTC GTACGGTCTG CCGGAACACG AGTTCCTGCT GGTCTTGGAA 
CACTTGTGCG CCGGAATGAA GCACTTGCGG GATAACAAGC TGGTGCATCG CGATCTGAAG 
CCCGGAAACA TAATGAAGTT CATCTCGGAG GACGGGCAAA CCATATACAA GCTTACTGAT 
TTCGGTGCTG CTAGAGAACT GGAGGATAAT CAGCCGTTTG CCTCTCTATA CGGCACAGAA 
GAGTATCTTC ATCCCGATCT CTACGAGCGC GCTGTGCTGA GGAAGTCAAT CCAGCGATCG 
TTCACCGCCA ATGTGGATTT GTGGTCGATT GGGGTCACGC TCTACCATGT GGCCACCGGA 
AATCTGCCTT TTAGACCTTT TGGTGGAAGG AAAAACCGAG AGACCATGCA CCAAATCACT 
ACCAAAAAGG CTTCTGGGGT GATTTCCGGC ACTCAGCTGT CCGAGAATGG ACCAATTGAA 
TGGTCCACCA CGTTGCCACC GCACGCCCAT CTCTCGCAGG GACTGAAAAC CCTGGTGACG 
CCTCTTCTAG CTGGACTTCT CGAGGAGAAT AGGGAAAAGA CCTGGTCTTT CGATCGTTTC 
TTCCACGAGG TGACGCTCAT CCTCCGCAAG CGTGTCATTC ATGTGTTCTT CACCAATCGG 
ACTAGTTCGG TGGAAGTGTT CCTAGAGCCA GACGAGCAAA TTGACAACTT CCGAGAGCGT 
ATTTTCCTGC AAACAGAGGT GCCGCTGGAG AAGCAGATCC TTTTGTTCAA CAACGAGCAT 
CTGGAGAAGA AGGTTACTCC ACGAACGATA GCTAAAGCAT TCCCTGCCAC CACAACAGAT 
CAGCCAATAT TCCTCTACAG CAACGACGAC AACAATGTTC AGTTGCCCCA GCAATTAGAT 
CTCCCCAAGT TCCCAGTGTT CCCTCCCAAT GTTTCAGTGG AGAACGACGC CAGTCTGGCA 
AAGTCCGCTT GCAGCGTTGG CCATGAATGC AAAAGACGCG TGGACATCTT TACTTCCATG 
GATATCCTTA TTAAGAAGGG AGTGGAGCAC TTCATAGAGA TGCTAGTGAC CACAATAACT 
CTACTATTGA AAAAAACTGA GAGTTTTGAC AACTTGCTTT CCACTGTGAT TGATTATGCA 
GATGTGGTGC ACAGCATGGC CAGAGTGACT AAAGGAGATC AGGAGATAAA GACCCTGCTG 
ACTGCCTTGG AAAATGTCAA AAGCGACTTT GATGGAGCCG CGGATGTGAT CTCGCAGATG 
CACAAACATT TTGTGATAGA TGACGAACTT AACGATCAAT GGACCTCCTC CATGCACGGA 
AAGAAGTGTC CTTGCAAGAC CAGAGCCAGT GCCCAGGCCA AGTATCTGGT AGAAAGGCTG 
CGGGACTCCT GGCAGCACTT GCTCCGGGAT CGTGCAACGC GCACACTGAC CTACAACGAC 
GAACAGTTTC ATGCCCTGGA GAAGATTAAA GTGGATCACA ATGGCAAACG GATAAAGGCC 
TTGCTTTTGG ATAACGTAAA TCCGACAGTG GCACAAATCG CAGAGTGCCT GGCGGACTGG 
TATAAGTTGG CTCAGACCGT CTACCTTAAA ACTCAAATTT TGGAGAAGGA CGTGCGCGAT 
TGCGAAAGAA AGTTGAACGG AATACGCGAT GAGTTGTATC ACGTTAAGTC GGAGCTGAAG 
CTGGATGTGG ACACAAAGAC CATAAACAAC AATAATCAGC TGGCCAAGAT AGAGGAGCGG 
AATCGGCTAA GGGTCATGCA GCAGCAGCAA CAGGAAGTTA TGGCTGTCAT GAGAACGAAC 
AGCGATATAA TAAGTTTGCT TAGCAAACTG GGCATTACCA ACGGAAGTCT GGAAAGTAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610048.2
CDS	181..2343
Translation	MSFLRGSVSYVWCTTSVLGKGATGSVFQGVNKITGESVAVKTFNPYSHMRPADVQMREFEALKKVNHENIVKLLAIEEDQEGRGKVIVMELCTGGSLFNILDDPENSYGLPEHEFLLVLEHLCAGMKHLRDNKLVHRDLKPGNIMKFISEDGQTIYKLTDFGAARELEDNQPFASLYGTEEYLHPDLYERAVLRKSIQRSFTANVDLWSIGVTLYHVATGNLPFRPFGGRKNRETMHQITTKKASGVISGTQLSENGPIEWSTTLPPHAHLSQGLKTLVTPLLAGLLEENREKTWSFDRFFHEVTLILRKRVIHVFFTNRTSSVEVFLEPDEQIDNFRERIFLQTEVPLEKQILLFNNEHLEKKVTPRTIAKAFPATTTDQPIFLYSNDDNNVQLPQQLDLPKFPVFPPNVSVENDASLAKSACSVGHECKRRVDIFTSMDILIKKGVEHFIEMLVTTITLLLKKTESFDNLLSTVIDYADVVHSMARVTKGDQEIKTLLTALENVKSDFDGAADVISQMHKHFVIDDELNDQWTSSMHGKKCPCKTRASAQAKYLVERLRDSWQHLLRDRATRTLTYNDEQFHALEKIKVDHNGKRIKALLLDNVNPTVAQIAECLADWYKLAQTVYLKTQILEKDVRDCERKLNGIRDELYHVKSELKLDVDTKTINNNNQLAKIEERNRLRVMQQQQQEVMAVMRTNSDIISLLSKLGITNGSLESS

Target ORF information:

RefSeq Version	NM_136204.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster I-kappaB kinase epsilon (IKKepsilon), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136204.3

ORF Insert Sequence:

ATGTCCTTCC TGCGCGGTTC CGTGAGCTAT GTGTGGTGCA CCACCAGCGT CCTGGGAAAG 
GGAGCCACCG GTTCCGTGTT CCAGGGGGTC AACAAGATCA CCGGCGAATC GGTGGCGGTG 
AAGACCTTTA ATCCCTACAG TCACATGCGA CCGGCTGATG TGCAGATGCG GGAGTTCGAG 
GCCCTGAAAA AGGTCAACCA CGAGAATATA GTAAAGCTGT TGGCGATCGA GGAGGATCAA 
GAGGGGCGTG GTAAGGTGAT CGTGATGGAG CTCTGCACAG GCGGAAGTCT CTTTAACATC 
CTGGACGATC CTGAGAACTC GTACGGTCTG CCGGAACACG AGTTCCTGCT GGTCTTGGAA 
CACTTGTGCG CCGGAATGAA GCACTTGCGG GATAACAAGC TGGTGCATCG CGATCTGAAG 
CCCGGAAACA TAATGAAGTT CATCTCGGAG GACGGGCAAA CCATATACAA GCTTACTGAT 
TTCGGTGCTG CTAGAGAACT GGAGGATAAT CAGCCGTTTG CCTCTCTATA CGGCACAGAA 
GAGTATCTTC ATCCCGATCT CTACGAGCGC GCTGTGCTGA GGAAGTCAAT CCAGCGATCG 
TTCACCGCCA ATGTGGATTT GTGGTCGATT GGGGTCACGC TCTACCATGT GGCCACCGGA 
AATCTGCCTT TTAGACCTTT TGGTGGAAGG AAAAACCGAG AGACCATGCA CCAAATCACT 
ACCAAAAAGG CTTCTGGGGT GATTTCCGGC ACTCAGCTGT CCGAGAATGG ACCAATTGAA 
TGGTCCACCA CGTTGCCACC GCACGCCCAT CTCTCGCAGG GACTGAAAAC CCTGGTGACG 
CCTCTTCTAG CTGGACTTCT CGAGGAGAAT AGGGAAAAGA CCTGGTCTTT CGATCGTTTC 
TTCCACGAGG TGACGCTCAT CCTCCGCAAG CGTGTCATTC ATGTGTTCTT CACCAATCGG 
ACTAGTTCGG TGGAAGTGTT CCTAGAGCCA GACGAGCAAA TTGACAACTT CCGAGAGCGT 
ATTTTCCTGC AAACAGAGGT GCCGCTGGAG AAGCAGATCC TTTTGTTCAA CAACGAGCAT 
CTGGAGAAGA AGGTTACTCC ACGAACGATA GCTAAAGCAT TCCCTGCCAC CACAACAGAT 
CAGCCAATAT TCCTCTACAG CAACGACGAC AACAATGTTC AGTTGCCCCA GCAATTAGAT 
CTCCCCAAGT TCCCAGTGTT CCCTCCCAAT GTTTCAGTGG AGAACGACGC CAGTCTGGCA 
AAGTCCGCTT GCAGCGTTGG CCATGAATGC AAAAGACGCG TGGACATCTT TACTTCCATG 
GATATCCTTA TTAAGAAGGG AGTGGAGCAC TTCATAGAGA TGCTAGTGAC CACAATAACT 
CTACTATTGA AAAAAACTGA GAGTTTTGAC AACTTGCTTT CCACTGTGAT TGATTATGCA 
GATGTGGTGC ACAGCATGGC CAGAGTGACT AAAGGAGATC AGGAGATAAA GACCCTGCTG 
ACTGCCTTGG AAAATGTCAA AAGCGACTTT GATGGAGCCG CGGATGTGAT CTCGCAGATG 
CACAAACATT TTGTGATAGA TGACGAACTT AACGATCAAT GGACCTCCTC CATGCACGGA 
AAGAAGTGTC CTTGCAAGAC CAGAGCCAGT GCCCAGGCCA AGTATCTGGT AGAAAGGCTG 
CGGGACTCCT GGCAGCACTT GCTCCGGGAT CGTGCAACGC GCACACTGAC CTACAACGAC 
GAACAGTTTC ATGCCCTGGA GAAGATTAAA GTGGATCACA ATGGCAAACG GATAAAGGCC 
TTGCTTTTGG ATAACGTAAA TCCGACAGTG GCACAAATCG CAGAGTGCCT GGCGGACTGG 
TATAAGTTGG CTCAGACCGT CTACCTTAAA ACTCAAATTT TGGAGAAGGA CGTGCGCGAT 
TGCGAAAGAA AGTTGAACGG AATACGCGAT GAGTTGTATC ACGTTAAGTC GGAGCTGAAG 
CTGGATGTGG ACACAAAGAC CATAAACAAC AATAATCAGC TGGCCAAGAT AGAGGAGCGG 
AATCGGCTAA GGGTCATGCA GCAGCAGCAA CAGGAAGTTA TGGCTGTCAT GAGAACGAAC 
AGCGATATAA TAAGTTTGCT TAGCAAACTG GGCATTACCA ACGGAAGTCT GGAAAGTAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IKKepsilon ( NM_165337.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724278.1 Drosophila melanogaster I-kappaB kinase epsilon (IKKepsilon), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15048
Clone ID Related Accession (Same CDS sequence)	NM_136204.3 , NM_165337.2
Accession Version	NM_165337.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	I-kappaB kinase epsilon, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165337.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCTTCC TGCGCGGTTC CGTGAGCTAT GTGTGGTGCA CCACCAGCGT CCTGGGAAAG 
GGAGCCACCG GTTCCGTGTT CCAGGGGGTC AACAAGATCA CCGGCGAATC GGTGGCGGTG 
AAGACCTTTA ATCCCTACAG TCACATGCGA CCGGCTGATG TGCAGATGCG GGAGTTCGAG 
GCCCTGAAAA AGGTCAACCA CGAGAATATA GTAAAGCTGT TGGCGATCGA GGAGGATCAA 
GAGGGGCGTG GTAAGGTGAT CGTGATGGAG CTCTGCACAG GCGGAAGTCT CTTTAACATC 
CTGGACGATC CTGAGAACTC GTACGGTCTG CCGGAACACG AGTTCCTGCT GGTCTTGGAA 
CACTTGTGCG CCGGAATGAA GCACTTGCGG GATAACAAGC TGGTGCATCG CGATCTGAAG 
CCCGGAAACA TAATGAAGTT CATCTCGGAG GACGGGCAAA CCATATACAA GCTTACTGAT 
TTCGGTGCTG CTAGAGAACT GGAGGATAAT CAGCCGTTTG CCTCTCTATA CGGCACAGAA 
GAGTATCTTC ATCCCGATCT CTACGAGCGC GCTGTGCTGA GGAAGTCAAT CCAGCGATCG 
TTCACCGCCA ATGTGGATTT GTGGTCGATT GGGGTCACGC TCTACCATGT GGCCACCGGA 
AATCTGCCTT TTAGACCTTT TGGTGGAAGG AAAAACCGAG AGACCATGCA CCAAATCACT 
ACCAAAAAGG CTTCTGGGGT GATTTCCGGC ACTCAGCTGT CCGAGAATGG ACCAATTGAA 
TGGTCCACCA CGTTGCCACC GCACGCCCAT CTCTCGCAGG GACTGAAAAC CCTGGTGACG 
CCTCTTCTAG CTGGACTTCT CGAGGAGAAT AGGGAAAAGA CCTGGTCTTT CGATCGTTTC 
TTCCACGAGG TGACGCTCAT CCTCCGCAAG CGTGTCATTC ATGTGTTCTT CACCAATCGG 
ACTAGTTCGG TGGAAGTGTT CCTAGAGCCA GACGAGCAAA TTGACAACTT CCGAGAGCGT 
ATTTTCCTGC AAACAGAGGT GCCGCTGGAG AAGCAGATCC TTTTGTTCAA CAACGAGCAT 
CTGGAGAAGA AGGTTACTCC ACGAACGATA GCTAAAGCAT TCCCTGCCAC CACAACAGAT 
CAGCCAATAT TCCTCTACAG CAACGACGAC AACAATGTTC AGTTGCCCCA GCAATTAGAT 
CTCCCCAAGT TCCCAGTGTT CCCTCCCAAT GTTTCAGTGG AGAACGACGC CAGTCTGGCA 
AAGTCCGCTT GCAGCGTTGG CCATGAATGC AAAAGACGCG TGGACATCTT TACTTCCATG 
GATATCCTTA TTAAGAAGGG AGTGGAGCAC TTCATAGAGA TGCTAGTGAC CACAATAACT 
CTACTATTGA AAAAAACTGA GAGTTTTGAC AACTTGCTTT CCACTGTGAT TGATTATGCA 
GATGTGGTGC ACAGCATGGC CAGAGTGACT AAAGGAGATC AGGAGATAAA GACCCTGCTG 
ACTGCCTTGG AAAATGTCAA AAGCGACTTT GATGGAGCCG CGGATGTGAT CTCGCAGATG 
CACAAACATT TTGTGATAGA TGACGAACTT AACGATCAAT GGACCTCCTC CATGCACGGA 
AAGAAGTGTC CTTGCAAGAC CAGAGCCAGT GCCCAGGCCA AGTATCTGGT AGAAAGGCTG 
CGGGACTCCT GGCAGCACTT GCTCCGGGAT CGTGCAACGC GCACACTGAC CTACAACGAC 
GAACAGTTTC ATGCCCTGGA GAAGATTAAA GTGGATCACA ATGGCAAACG GATAAAGGCC 
TTGCTTTTGG ATAACGTAAA TCCGACAGTG GCACAAATCG CAGAGTGCCT GGCGGACTGG 
TATAAGTTGG CTCAGACCGT CTACCTTAAA ACTCAAATTT TGGAGAAGGA CGTGCGCGAT 
TGCGAAAGAA AGTTGAACGG AATACGCGAT GAGTTGTATC ACGTTAAGTC GGAGCTGAAG 
CTGGATGTGG ACACAAAGAC CATAAACAAC AATAATCAGC TGGCCAAGAT AGAGGAGCGG 
AATCGGCTAA GGGTCATGCA GCAGCAGCAA CAGGAAGTTA TGGCTGTCAT GAGAACGAAC 
AGCGATATAA TAAGTTTGCT TAGCAAACTG GGCATTACCA ACGGAAGTCT GGAAAGTAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724278.1
CDS	91..2253
Translation	MSFLRGSVSYVWCTTSVLGKGATGSVFQGVNKITGESVAVKTFNPYSHMRPADVQMREFEALKKVNHENIVKLLAIEEDQEGRGKVIVMELCTGGSLFNILDDPENSYGLPEHEFLLVLEHLCAGMKHLRDNKLVHRDLKPGNIMKFISEDGQTIYKLTDFGAARELEDNQPFASLYGTEEYLHPDLYERAVLRKSIQRSFTANVDLWSIGVTLYHVATGNLPFRPFGGRKNRETMHQITTKKASGVISGTQLSENGPIEWSTTLPPHAHLSQGLKTLVTPLLAGLLEENREKTWSFDRFFHEVTLILRKRVIHVFFTNRTSSVEVFLEPDEQIDNFRERIFLQTEVPLEKQILLFNNEHLEKKVTPRTIAKAFPATTTDQPIFLYSNDDNNVQLPQQLDLPKFPVFPPNVSVENDASLAKSACSVGHECKRRVDIFTSMDILIKKGVEHFIEMLVTTITLLLKKTESFDNLLSTVIDYADVVHSMARVTKGDQEIKTLLTALENVKSDFDGAADVISQMHKHFVIDDELNDQWTSSMHGKKCPCKTRASAQAKYLVERLRDSWQHLLRDRATRTLTYNDEQFHALEKIKVDHNGKRIKALLLDNVNPTVAQIAECLADWYKLAQTVYLKTQILEKDVRDCERKLNGIRDELYHVKSELKLDVDTKTINNNNQLAKIEERNRLRVMQQQQQEVMAVMRTNSDIISLLSKLGITNGSLESS

Target ORF information:

RefSeq Version	NM_165337.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster I-kappaB kinase epsilon (IKKepsilon), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165337.2

ORF Insert Sequence:

ATGTCCTTCC TGCGCGGTTC CGTGAGCTAT GTGTGGTGCA CCACCAGCGT CCTGGGAAAG 
GGAGCCACCG GTTCCGTGTT CCAGGGGGTC AACAAGATCA CCGGCGAATC GGTGGCGGTG 
AAGACCTTTA ATCCCTACAG TCACATGCGA CCGGCTGATG TGCAGATGCG GGAGTTCGAG 
GCCCTGAAAA AGGTCAACCA CGAGAATATA GTAAAGCTGT TGGCGATCGA GGAGGATCAA 
GAGGGGCGTG GTAAGGTGAT CGTGATGGAG CTCTGCACAG GCGGAAGTCT CTTTAACATC 
CTGGACGATC CTGAGAACTC GTACGGTCTG CCGGAACACG AGTTCCTGCT GGTCTTGGAA 
CACTTGTGCG CCGGAATGAA GCACTTGCGG GATAACAAGC TGGTGCATCG CGATCTGAAG 
CCCGGAAACA TAATGAAGTT CATCTCGGAG GACGGGCAAA CCATATACAA GCTTACTGAT 
TTCGGTGCTG CTAGAGAACT GGAGGATAAT CAGCCGTTTG CCTCTCTATA CGGCACAGAA 
GAGTATCTTC ATCCCGATCT CTACGAGCGC GCTGTGCTGA GGAAGTCAAT CCAGCGATCG 
TTCACCGCCA ATGTGGATTT GTGGTCGATT GGGGTCACGC TCTACCATGT GGCCACCGGA 
AATCTGCCTT TTAGACCTTT TGGTGGAAGG AAAAACCGAG AGACCATGCA CCAAATCACT 
ACCAAAAAGG CTTCTGGGGT GATTTCCGGC ACTCAGCTGT CCGAGAATGG ACCAATTGAA 
TGGTCCACCA CGTTGCCACC GCACGCCCAT CTCTCGCAGG GACTGAAAAC CCTGGTGACG 
CCTCTTCTAG CTGGACTTCT CGAGGAGAAT AGGGAAAAGA CCTGGTCTTT CGATCGTTTC 
TTCCACGAGG TGACGCTCAT CCTCCGCAAG CGTGTCATTC ATGTGTTCTT CACCAATCGG 
ACTAGTTCGG TGGAAGTGTT CCTAGAGCCA GACGAGCAAA TTGACAACTT CCGAGAGCGT 
ATTTTCCTGC AAACAGAGGT GCCGCTGGAG AAGCAGATCC TTTTGTTCAA CAACGAGCAT 
CTGGAGAAGA AGGTTACTCC ACGAACGATA GCTAAAGCAT TCCCTGCCAC CACAACAGAT 
CAGCCAATAT TCCTCTACAG CAACGACGAC AACAATGTTC AGTTGCCCCA GCAATTAGAT 
CTCCCCAAGT TCCCAGTGTT CCCTCCCAAT GTTTCAGTGG AGAACGACGC CAGTCTGGCA 
AAGTCCGCTT GCAGCGTTGG CCATGAATGC AAAAGACGCG TGGACATCTT TACTTCCATG 
GATATCCTTA TTAAGAAGGG AGTGGAGCAC TTCATAGAGA TGCTAGTGAC CACAATAACT 
CTACTATTGA AAAAAACTGA GAGTTTTGAC AACTTGCTTT CCACTGTGAT TGATTATGCA 
GATGTGGTGC ACAGCATGGC CAGAGTGACT AAAGGAGATC AGGAGATAAA GACCCTGCTG 
ACTGCCTTGG AAAATGTCAA AAGCGACTTT GATGGAGCCG CGGATGTGAT CTCGCAGATG 
CACAAACATT TTGTGATAGA TGACGAACTT AACGATCAAT GGACCTCCTC CATGCACGGA 
AAGAAGTGTC CTTGCAAGAC CAGAGCCAGT GCCCAGGCCA AGTATCTGGT AGAAAGGCTG 
CGGGACTCCT GGCAGCACTT GCTCCGGGAT CGTGCAACGC GCACACTGAC CTACAACGAC 
GAACAGTTTC ATGCCCTGGA GAAGATTAAA GTGGATCACA ATGGCAAACG GATAAAGGCC 
TTGCTTTTGG ATAACGTAAA TCCGACAGTG GCACAAATCG CAGAGTGCCT GGCGGACTGG 
TATAAGTTGG CTCAGACCGT CTACCTTAAA ACTCAAATTT TGGAGAAGGA CGTGCGCGAT 
TGCGAAAGAA AGTTGAACGG AATACGCGAT GAGTTGTATC ACGTTAAGTC GGAGCTGAAG 
CTGGATGTGG ACACAAAGAC CATAAACAAC AATAATCAGC TGGCCAAGAT AGAGGAGCGG 
AATCGGCTAA GGGTCATGCA GCAGCAGCAA CAGGAAGTTA TGGCTGTCAT GAGAACGAAC 
AGCGATATAA TAAGTTTGCT TAGCAAACTG GGCATTACCA ACGGAAGTCT GGAAAGTAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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IKKepsilon cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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