Cdc23 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdc23
Entrez Gene ID	35315
Full Name	Cell division cycle 23
Synonyms	38C.54,APC8,Apc8,CDC23,CG2508,CG2508-PA,Dmel\CG2508,cdc23
General protein information	Preferred Names Cell division cycle 23 Names CG2508 gene product from transcript CG2508-RA CG2508-PA CG2508-PB Cdc23-PA Cdc23-PB cdc23 cell division cycle 23 cell division cycle 23 ortholog
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38C7-38C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_136192.3	NP_610036.2	cell division cycle 23, isoform A
NM_001273700.1	NP_001260629.1	cell division cycle 23, isoform B

Pathways from BioSystems

Homologies

	CDC23	XP_001112573.1
Rattus norvegicus (Norway rat)	Cdc23	NP_001094129.1
Drosophila melanogaster (fruit fly)	CG31687	NP_001163022.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009880	XP_319000.3
Saccharomyces cerevisiae (baker's yeast)	CDC23	NP_012036.1
Schizosaccharomyces pombe (fission yeast)	cut23	NP_593300.1
Bos taurus (cattle)	CDC23	NP_001073735.1
Gallus gallus (chicken)	CDC23	XP_003642151.1
Danio rerio (zebrafish)	cdc23	NP_957227.1
Kluyveromyces lactis	KLLA0A05258g	XP_451232.1
Magnaporthe oryzae (rice blast fungus)	MGG_08648	XP_003711081.1
Oryza sativa (rice)	Os02g0656300	NP_001047624.1
Homo sapiens (human)	CDC23	NP_004652.2
Neurospora crassa	NCU01174	XP_961540.2
Pan troglodytes (chimpanzee)	CDC23	XP_517953.2
Canis lupus familiaris (dog)	CDC23	XP_538647.2
Mus musculus (house mouse)	Cdc23	NP_848124.1
Drosophila melanogaster (fruit fly)	cdc23	NP_001260629.1
Eremothecium gossypii	AGOS_ABR206W	NP_983155.2
Arabidopsis thaliana (thale cress)	APC8	NP_190398.1
Xenopus tropicalis (tropical clawed frog)	cdc23	NP_001016759.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Apc6/Cdc16 and Apc8/Cdc23 silencing induces developmental delay and causes different pupal lethality
Title: Structurally related TPR subunits contribute differently to the function of the anaphase-promoting complex in Drosophila melanogaster.

The following Cdc23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdc23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15037	NM_136192.3 Latest version!	Drosophila melanogaster cell division cycle 23, transcript variant A (Cdc23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15037	NM_001273700.1 Latest version!	Drosophila melanogaster cell division cycle 23, transcript variant B (Cdc23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdc23 ( NM_136192.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610036.2 Drosophila melanogaster cell division cycle 23, transcript variant A (Cdc23), mRNA.

Price & Availability↑

CloneID	OFa15037
Clone ID Related Accession (Same CDS sequence)	NM_136192.3 , NM_001273700.1
Accession Version	NM_136192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cell division cycle 23, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_136192.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGATGC AGAAGTTCTT CAGCGTGCTG CTGCCGGATG TGAAGCGGGA GCTGCGCCGC 
GGGATCATCG AGTGCTCCAA GCGGGGCCTC CTGCACAGCA CCAAGTGGCT GGCGGAAATG 
CATCACGGAC TGGCCGATGT GCACATAGAC AATGAGGCGC CTGATGAGGA TCGCACATTC 
AGCGAGTGTC AGCTGGAGGG GATTGCGCCG GAGGAGTACA GCGACTACTT CCTGGCCAAG 
AGCTACTACG ACGTGAGGGA GTACGACAGG GCGGCGCATG CGGTCAGGAA CTGCGAGTCC 
AGTGTTCCGC GCTTCCTGCA CTTCTATTCC AGCTACATGG CCAGGGAAAA GCGGCGACTG 
GACTCGACCA CGGACCAGGC GAATCTGCAC GAACCGAATC AGATGCGCGA TCTCGCCGAC 
CTGCTGGCTA CGTTGCGCAT GGAATACGGC AAAAGTCGGC TCGATGGCTA CGGAATATAC 
TTATACGGAG TAGTCCTGAA AGCACTGAAT CTCAACCAGG CCGCCGAGCA AATGCTGGTC 
CAGGCCATCA GACTGGTACC CATGCTGTGG AGCGCCTATC TGGAGCTCTC GCCCCTCATC 
ATGGAGAAGA AGAAACTGCT GAGCCTGCAG CTGGGTGGTC ACTGGATGCG GCACTTCTTC 
ATGGCGCACA CTTACCTGGA GCTGTACCTC AACGACGACG GCCTGAAGAT CTACGAGGAC 
CTGCAGGCAT CGGGTTTCAG CAAGAGCATC TACTTGATTG CCCAGATGGC TCTGGTCTAT 
CACAATAAGC GGGATGTGGA CAAGGCGATT GAGCTGTACC AGGCGCTTCT CGAGAGCGAT 
CCCTACCGCC TGGACAATGT GGACACCTAT TCCAATCTGC TGTTCGTCAA GGAGATGAAA 
ACCGAAATGG CACAGTTGGC CCACAAGGCT GTCAGCATCA ACAAGTACCG CCCGGAGACG 
TGTTGTGTAA TAGGCAACTA CTACAGTATC CGCTGTGATC ACCAGGTGGC CATTTCCTAC 
TTTCAACGCG CCCTGAAACT GAATCCCAAG TATCTGGCGG CCTGGACCTT GATGGGACAC 
GAGTTCATGG AGCTGAAAAA CACAAATGCA GCCATACAGA GCTACCGAAA GGCAGTGGAG 
GTCAACAAGC GGGACTATCG CGCCTGGTAT GGACTGGGCC AGGCCTACGA GATCATTAAA 
ATGCACTACT ACAGCCTGTA CTACTTTAAA ATCGCCCACC AACTGCGTCC CTACGACTCT 
CGAATGCTGG TTGCCTTGGG CGAGACCTAT GAGAAGCTGG ACAAGTGCGA AAATGCTGTG 
AAGTGCTACT GGAAGGCCAT CGATGTGGGT GACATAGAGG GCATAGCGAT GTACAAACTG 
GCCAATCTGC ACGAGAAGCT CGGCGACCAC GAGACGGCGG TCCATTGCTA CATCATGTAC 
TGCGAGGATG AGCGGGCGGC CACCGATAAG CAGAGCCTCT ACCAAGGGTT CATCACGCTG 
GCCAATTATT ACGAGAAGAA GGGCGAGTAC GAACGAGCCG CCTATTACGC TTACAAATGC 
CTAGACTCAG AGGATCGCAA AATGGAGGCA AAGGCGCTGC TGAAGACGAT TGACTGGAAG 
CGCAATGCTG AGGGACAGAA GAAGGTAAAA ACTTCGACGG CCGTGGCGAA TGCAGATACC 
AGTTCCGAGG ACGAGATGGA GTGGGAGCTC CAGGATGTGC GAGTTCGAGT TCCCATCACA 
TCCATAGCCA CCACGACTAC CTCCACGACC ACCGTAGCTG CGGAGAGCGC CTCGAGTAGC 
TCCCTGATCA GCTTGCGTCC GGACCGAAAC CTGATTCAGG GCATGCGTCG ATCCCAGTCG 
AGCAGGACTA GCTTAACCGC GCCTGCCAGC GGCACCACCA CCACGCCATC GGCAACTGCT 
GTCACAATGT CCACCGAGGA AACGCCTGGC ACATCCGGTG GCCCTTCGAA TCCGCCCGAG 
CAGGCGCCCT CGGATGATAA CAGCTCCATG GAAATATCCA GCGTATCCAT CGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610036.2
CDS	77..2113
Translation	MAMQKFFSVLLPDVKRELRRGIIECSKRGLLHSTKWLAEMHHGLADVHIDNEAPDEDRTFSECQLEGIAPEEYSDYFLAKSYYDVREYDRAAHAVRNCESSVPRFLHFYSSYMAREKRRLDSTTDQANLHEPNQMRDLADLLATLRMEYGKSRLDGYGIYLYGVVLKALNLNQAAEQMLVQAIRLVPMLWSAYLELSPLIMEKKKLLSLQLGGHWMRHFFMAHTYLELYLNDDGLKIYEDLQASGFSKSIYLIAQMALVYHNKRDVDKAIELYQALLESDPYRLDNVDTYSNLLFVKEMKTEMAQLAHKAVSINKYRPETCCVIGNYYSIRCDHQVAISYFQRALKLNPKYLAAWTLMGHEFMELKNTNAAIQSYRKAVEVNKRDYRAWYGLGQAYEIIKMHYYSLYYFKIAHQLRPYDSRMLVALGETYEKLDKCENAVKCYWKAIDVGDIEGIAMYKLANLHEKLGDHETAVHCYIMYCEDERAATDKQSLYQGFITLANYYEKKGEYERAAYYAYKCLDSEDRKMEAKALLKTIDWKRNAEGQKKVKTSTAVANADTSSEDEMEWELQDVRVRVPITSIATTTTSTTTVAAESASSSSLISLRPDRNLIQGMRRSQSSRTSLTAPASGTTTTPSATAVTMSTEETPGTSGGPSNPPEQAPSDDNSSMEISSVSID

Target ORF information:

RefSeq Version	NM_136192.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cell division cycle 23, transcript variant A (Cdc23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136192.3

ORF Insert Sequence:

ATGGCGATGC AGAAGTTCTT CAGCGTGCTG CTGCCGGATG TGAAGCGGGA GCTGCGCCGC 
GGGATCATCG AGTGCTCCAA GCGGGGCCTC CTGCACAGCA CCAAGTGGCT GGCGGAAATG 
CATCACGGAC TGGCCGATGT GCACATAGAC AATGAGGCGC CTGATGAGGA TCGCACATTC 
AGCGAGTGTC AGCTGGAGGG GATTGCGCCG GAGGAGTACA GCGACTACTT CCTGGCCAAG 
AGCTACTACG ACGTGAGGGA GTACGACAGG GCGGCGCATG CGGTCAGGAA CTGCGAGTCC 
AGTGTTCCGC GCTTCCTGCA CTTCTATTCC AGCTACATGG CCAGGGAAAA GCGGCGACTG 
GACTCGACCA CGGACCAGGC GAATCTGCAC GAACCGAATC AGATGCGCGA TCTCGCCGAC 
CTGCTGGCTA CGTTGCGCAT GGAATACGGC AAAAGTCGGC TCGATGGCTA CGGAATATAC 
TTATACGGAG TAGTCCTGAA AGCACTGAAT CTCAACCAGG CCGCCGAGCA AATGCTGGTC 
CAGGCCATCA GACTGGTACC CATGCTGTGG AGCGCCTATC TGGAGCTCTC GCCCCTCATC 
ATGGAGAAGA AGAAACTGCT GAGCCTGCAG CTGGGTGGTC ACTGGATGCG GCACTTCTTC 
ATGGCGCACA CTTACCTGGA GCTGTACCTC AACGACGACG GCCTGAAGAT CTACGAGGAC 
CTGCAGGCAT CGGGTTTCAG CAAGAGCATC TACTTGATTG CCCAGATGGC TCTGGTCTAT 
CACAATAAGC GGGATGTGGA CAAGGCGATT GAGCTGTACC AGGCGCTTCT CGAGAGCGAT 
CCCTACCGCC TGGACAATGT GGACACCTAT TCCAATCTGC TGTTCGTCAA GGAGATGAAA 
ACCGAAATGG CACAGTTGGC CCACAAGGCT GTCAGCATCA ACAAGTACCG CCCGGAGACG 
TGTTGTGTAA TAGGCAACTA CTACAGTATC CGCTGTGATC ACCAGGTGGC CATTTCCTAC 
TTTCAACGCG CCCTGAAACT GAATCCCAAG TATCTGGCGG CCTGGACCTT GATGGGACAC 
GAGTTCATGG AGCTGAAAAA CACAAATGCA GCCATACAGA GCTACCGAAA GGCAGTGGAG 
GTCAACAAGC GGGACTATCG CGCCTGGTAT GGACTGGGCC AGGCCTACGA GATCATTAAA 
ATGCACTACT ACAGCCTGTA CTACTTTAAA ATCGCCCACC AACTGCGTCC CTACGACTCT 
CGAATGCTGG TTGCCTTGGG CGAGACCTAT GAGAAGCTGG ACAAGTGCGA AAATGCTGTG 
AAGTGCTACT GGAAGGCCAT CGATGTGGGT GACATAGAGG GCATAGCGAT GTACAAACTG 
GCCAATCTGC ACGAGAAGCT CGGCGACCAC GAGACGGCGG TCCATTGCTA CATCATGTAC 
TGCGAGGATG AGCGGGCGGC CACCGATAAG CAGAGCCTCT ACCAAGGGTT CATCACGCTG 
GCCAATTATT ACGAGAAGAA GGGCGAGTAC GAACGAGCCG CCTATTACGC TTACAAATGC 
CTAGACTCAG AGGATCGCAA AATGGAGGCA AAGGCGCTGC TGAAGACGAT TGACTGGAAG 
CGCAATGCTG AGGGACAGAA GAAGGTAAAA ACTTCGACGG CCGTGGCGAA TGCAGATACC 
AGTTCCGAGG ACGAGATGGA GTGGGAGCTC CAGGATGTGC GAGTTCGAGT TCCCATCACA 
TCCATAGCCA CCACGACTAC CTCCACGACC ACCGTAGCTG CGGAGAGCGC CTCGAGTAGC 
TCCCTGATCA GCTTGCGTCC GGACCGAAAC CTGATTCAGG GCATGCGTCG ATCCCAGTCG 
AGCAGGACTA GCTTAACCGC GCCTGCCAGC GGCACCACCA CCACGCCATC GGCAACTGCT 
GTCACAATGT CCACCGAGGA AACGCCTGGC ACATCCGGTG GCCCTTCGAA TCCGCCCGAG 
CAGGCGCCCT CGGATGATAA CAGCTCCATG GAAATATCCA GCGTATCCAT CGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc23 ( NM_001273700.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260629.1 Drosophila melanogaster cell division cycle 23, transcript variant B (Cdc23), mRNA.

Price & Availability↑

CloneID	OFa15037
Clone ID Related Accession (Same CDS sequence)	NM_136192.3 , NM_001273700.1
Accession Version	NM_001273700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cell division cycle 23, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGATGC AGAAGTTCTT CAGCGTGCTG CTGCCGGATG TGAAGCGGGA GCTGCGCCGC 
GGGATCATCG AGTGCTCCAA GCGGGGCCTC CTGCACAGCA CCAAGTGGCT GGCGGAAATG 
CATCACGGAC TGGCCGATGT GCACATAGAC AATGAGGCGC CTGATGAGGA TCGCACATTC 
AGCGAGTGTC AGCTGGAGGG GATTGCGCCG GAGGAGTACA GCGACTACTT CCTGGCCAAG 
AGCTACTACG ACGTGAGGGA GTACGACAGG GCGGCGCATG CGGTCAGGAA CTGCGAGTCC 
AGTGTTCCGC GCTTCCTGCA CTTCTATTCC AGCTACATGG CCAGGGAAAA GCGGCGACTG 
GACTCGACCA CGGACCAGGC GAATCTGCAC GAACCGAATC AGATGCGCGA TCTCGCCGAC 
CTGCTGGCTA CGTTGCGCAT GGAATACGGC AAAAGTCGGC TCGATGGCTA CGGAATATAC 
TTATACGGAG TAGTCCTGAA AGCACTGAAT CTCAACCAGG CCGCCGAGCA AATGCTGGTC 
CAGGCCATCA GACTGGTACC CATGCTGTGG AGCGCCTATC TGGAGCTCTC GCCCCTCATC 
ATGGAGAAGA AGAAACTGCT GAGCCTGCAG CTGGGTGGTC ACTGGATGCG GCACTTCTTC 
ATGGCGCACA CTTACCTGGA GCTGTACCTC AACGACGACG GCCTGAAGAT CTACGAGGAC 
CTGCAGGCAT CGGGTTTCAG CAAGAGCATC TACTTGATTG CCCAGATGGC TCTGGTCTAT 
CACAATAAGC GGGATGTGGA CAAGGCGATT GAGCTGTACC AGGCGCTTCT CGAGAGCGAT 
CCCTACCGCC TGGACAATGT GGACACCTAT TCCAATCTGC TGTTCGTCAA GGAGATGAAA 
ACCGAAATGG CACAGTTGGC CCACAAGGCT GTCAGCATCA ACAAGTACCG CCCGGAGACG 
TGTTGTGTAA TAGGCAACTA CTACAGTATC CGCTGTGATC ACCAGGTGGC CATTTCCTAC 
TTTCAACGCG CCCTGAAACT GAATCCCAAG TATCTGGCGG CCTGGACCTT GATGGGACAC 
GAGTTCATGG AGCTGAAAAA CACAAATGCA GCCATACAGA GCTACCGAAA GGCAGTGGAG 
GTCAACAAGC GGGACTATCG CGCCTGGTAT GGACTGGGCC AGGCCTACGA GATCATTAAA 
ATGCACTACT ACAGCCTGTA CTACTTTAAA ATCGCCCACC AACTGCGTCC CTACGACTCT 
CGAATGCTGG TTGCCTTGGG CGAGACCTAT GAGAAGCTGG ACAAGTGCGA AAATGCTGTG 
AAGTGCTACT GGAAGGCCAT CGATGTGGGT GACATAGAGG GCATAGCGAT GTACAAACTG 
GCCAATCTGC ACGAGAAGCT CGGCGACCAC GAGACGGCGG TCCATTGCTA CATCATGTAC 
TGCGAGGATG AGCGGGCGGC CACCGATAAG CAGAGCCTCT ACCAAGGGTT CATCACGCTG 
GCCAATTATT ACGAGAAGAA GGGCGAGTAC GAACGAGCCG CCTATTACGC TTACAAATGC 
CTAGACTCAG AGGATCGCAA AATGGAGGCA AAGGCGCTGC TGAAGACGAT TGACTGGAAG 
CGCAATGCTG AGGGACAGAA GAAGGTAAAA ACTTCGACGG CCGTGGCGAA TGCAGATACC 
AGTTCCGAGG ACGAGATGGA GTGGGAGCTC CAGGATGTGC GAGTTCGAGT TCCCATCACA 
TCCATAGCCA CCACGACTAC CTCCACGACC ACCGTAGCTG CGGAGAGCGC CTCGAGTAGC 
TCCCTGATCA GCTTGCGTCC GGACCGAAAC CTGATTCAGG GCATGCGTCG ATCCCAGTCG 
AGCAGGACTA GCTTAACCGC GCCTGCCAGC GGCACCACCA CCACGCCATC GGCAACTGCT 
GTCACAATGT CCACCGAGGA AACGCCTGGC ACATCCGGTG GCCCTTCGAA TCCGCCCGAG 
CAGGCGCCCT CGGATGATAA CAGCTCCATG GAAATATCCA GCGTATCCAT CGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260629.1
CDS	77..2113
Translation	MAMQKFFSVLLPDVKRELRRGIIECSKRGLLHSTKWLAEMHHGLADVHIDNEAPDEDRTFSECQLEGIAPEEYSDYFLAKSYYDVREYDRAAHAVRNCESSVPRFLHFYSSYMAREKRRLDSTTDQANLHEPNQMRDLADLLATLRMEYGKSRLDGYGIYLYGVVLKALNLNQAAEQMLVQAIRLVPMLWSAYLELSPLIMEKKKLLSLQLGGHWMRHFFMAHTYLELYLNDDGLKIYEDLQASGFSKSIYLIAQMALVYHNKRDVDKAIELYQALLESDPYRLDNVDTYSNLLFVKEMKTEMAQLAHKAVSINKYRPETCCVIGNYYSIRCDHQVAISYFQRALKLNPKYLAAWTLMGHEFMELKNTNAAIQSYRKAVEVNKRDYRAWYGLGQAYEIIKMHYYSLYYFKIAHQLRPYDSRMLVALGETYEKLDKCENAVKCYWKAIDVGDIEGIAMYKLANLHEKLGDHETAVHCYIMYCEDERAATDKQSLYQGFITLANYYEKKGEYERAAYYAYKCLDSEDRKMEAKALLKTIDWKRNAEGQKKVKTSTAVANADTSSEDEMEWELQDVRVRVPITSIATTTTSTTTVAAESASSSSLISLRPDRNLIQGMRRSQSSRTSLTAPASGTTTTPSATAVTMSTEETPGTSGGPSNPPEQAPSDDNSSMEISSVSID

Target ORF information:

RefSeq Version	NM_001273700.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cell division cycle 23, transcript variant B (Cdc23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273700.1

ORF Insert Sequence:

ATGGCGATGC AGAAGTTCTT CAGCGTGCTG CTGCCGGATG TGAAGCGGGA GCTGCGCCGC 
GGGATCATCG AGTGCTCCAA GCGGGGCCTC CTGCACAGCA CCAAGTGGCT GGCGGAAATG 
CATCACGGAC TGGCCGATGT GCACATAGAC AATGAGGCGC CTGATGAGGA TCGCACATTC 
AGCGAGTGTC AGCTGGAGGG GATTGCGCCG GAGGAGTACA GCGACTACTT CCTGGCCAAG 
AGCTACTACG ACGTGAGGGA GTACGACAGG GCGGCGCATG CGGTCAGGAA CTGCGAGTCC 
AGTGTTCCGC GCTTCCTGCA CTTCTATTCC AGCTACATGG CCAGGGAAAA GCGGCGACTG 
GACTCGACCA CGGACCAGGC GAATCTGCAC GAACCGAATC AGATGCGCGA TCTCGCCGAC 
CTGCTGGCTA CGTTGCGCAT GGAATACGGC AAAAGTCGGC TCGATGGCTA CGGAATATAC 
TTATACGGAG TAGTCCTGAA AGCACTGAAT CTCAACCAGG CCGCCGAGCA AATGCTGGTC 
CAGGCCATCA GACTGGTACC CATGCTGTGG AGCGCCTATC TGGAGCTCTC GCCCCTCATC 
ATGGAGAAGA AGAAACTGCT GAGCCTGCAG CTGGGTGGTC ACTGGATGCG GCACTTCTTC 
ATGGCGCACA CTTACCTGGA GCTGTACCTC AACGACGACG GCCTGAAGAT CTACGAGGAC 
CTGCAGGCAT CGGGTTTCAG CAAGAGCATC TACTTGATTG CCCAGATGGC TCTGGTCTAT 
CACAATAAGC GGGATGTGGA CAAGGCGATT GAGCTGTACC AGGCGCTTCT CGAGAGCGAT 
CCCTACCGCC TGGACAATGT GGACACCTAT TCCAATCTGC TGTTCGTCAA GGAGATGAAA 
ACCGAAATGG CACAGTTGGC CCACAAGGCT GTCAGCATCA ACAAGTACCG CCCGGAGACG 
TGTTGTGTAA TAGGCAACTA CTACAGTATC CGCTGTGATC ACCAGGTGGC CATTTCCTAC 
TTTCAACGCG CCCTGAAACT GAATCCCAAG TATCTGGCGG CCTGGACCTT GATGGGACAC 
GAGTTCATGG AGCTGAAAAA CACAAATGCA GCCATACAGA GCTACCGAAA GGCAGTGGAG 
GTCAACAAGC GGGACTATCG CGCCTGGTAT GGACTGGGCC AGGCCTACGA GATCATTAAA 
ATGCACTACT ACAGCCTGTA CTACTTTAAA ATCGCCCACC AACTGCGTCC CTACGACTCT 
CGAATGCTGG TTGCCTTGGG CGAGACCTAT GAGAAGCTGG ACAAGTGCGA AAATGCTGTG 
AAGTGCTACT GGAAGGCCAT CGATGTGGGT GACATAGAGG GCATAGCGAT GTACAAACTG 
GCCAATCTGC ACGAGAAGCT CGGCGACCAC GAGACGGCGG TCCATTGCTA CATCATGTAC 
TGCGAGGATG AGCGGGCGGC CACCGATAAG CAGAGCCTCT ACCAAGGGTT CATCACGCTG 
GCCAATTATT ACGAGAAGAA GGGCGAGTAC GAACGAGCCG CCTATTACGC TTACAAATGC 
CTAGACTCAG AGGATCGCAA AATGGAGGCA AAGGCGCTGC TGAAGACGAT TGACTGGAAG 
CGCAATGCTG AGGGACAGAA GAAGGTAAAA ACTTCGACGG CCGTGGCGAA TGCAGATACC 
AGTTCCGAGG ACGAGATGGA GTGGGAGCTC CAGGATGTGC GAGTTCGAGT TCCCATCACA 
TCCATAGCCA CCACGACTAC CTCCACGACC ACCGTAGCTG CGGAGAGCGC CTCGAGTAGC 
TCCCTGATCA GCTTGCGTCC GGACCGAAAC CTGATTCAGG GCATGCGTCG ATCCCAGTCG 
AGCAGGACTA GCTTAACCGC GCCTGCCAGC GGCACCACCA CCACGCCATC GGCAACTGCT 
GTCACAATGT CCACCGAGGA AACGCCTGGC ACATCCGGTG GCCCTTCGAA TCCGCCCGAG 
CAGGCGCCCT CGGATGATAA CAGCTCCATG GAAATATCCA GCGTATCCAT CGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
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Combined evidence annotation of transposable elements in genome sequences.
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Cdc23 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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