RtGEF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RtGEF
Entrez Gene ID	35306
Full Name	Rho-type guanine nucleotide exchange factor
Synonyms	38C.40,ARHGEF7,CG10043,Dmel\CG10043,DrhoGEF,DrtGEF,Pix,Rho/RacGEF,RhoGEF,beta-PIX,betaPIX,dPix,dpix,pix,rhoGEF,rtGEF
General protein information	Preferred Names Rho-type guanine nucleotide exchange factor Names CG10043 gene product from transcript CG10043-RD CG10043-PA CG10043-PB CG10043-PD CG10043-PE CG10043-PF CG10043-PG CG10043-PH PAK-interacting exchange factor RtGEF-PA RtGEF-PB RtGEF-PD RtGEF-PE RtGEF-PF RtGEF-PG RtGEF-PH drosophila rho-type guanine exchange factor rho-type guanine exchange factor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38C5-38C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_057826.5	NP_477174.1	Rho-type guanine nucleotide exchange factor, isoform A
NM_001273694.1	NP_001260623.1	Rho-type guanine nucleotide exchange factor, isoform H
NM_001103715.4	NP_001097185.2	Rho-type guanine nucleotide exchange factor, isoform D
NM_165327.3	NP_724258.1	Rho-type guanine nucleotide exchange factor, isoform B
NM_001273693.1	NP_001260622.1	Rho-type guanine nucleotide exchange factor, isoform G
NM_001169550.2	NP_001163021.1	Rho-type guanine nucleotide exchange factor, isoform E
NM_001103714.3	NP_001097184.2	Rho-type guanine nucleotide exchange factor, isoform F

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	rtGEF	NP_724258.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007877	XP_317615.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pix and Git regulate Hippo-pathway-dependent tissue growth.
Title: The GTPase regulatory proteins Pix and Git control tissue growth via the Hippo pathway.

A novel RhoGEF(Pbl)-dependent input promotes the simultaneous association of anillin with the plasma membrane, septins, and microtubules, independently of F-actin.
Title: Rho-dependent control of anillin behavior during cytokinesis.

The following RtGEF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RtGEF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15028	NM_001273694.1 Latest version!	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa16898	NM_001103714.3 Latest version!	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa15027	NM_001169550.2 Latest version!	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa15029	NM_165327.3 Latest version!	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa15029	NM_057826.5 Latest version!	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa16939	NM_001103715.4 Latest version!	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa16944	NM_001273693.1 Latest version!	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RtGEF ( NM_001273694.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260623.1 Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa15028
Clone ID Related Accession (Same CDS sequence)	NM_001273694.1
Accession Version	NM_001273694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho-type guanine nucleotide exchange factor, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
CTGAGTCAAC ATTTGGATTC ACGGGGCTAC TGCACGCGAT TCTCGCTGTG TGCCTATTAC 
TCCAGCCCCC CGTGCCATGT GCGACCTCTC CGTGTGACTC TTCCCCCAAG CAATTACCCA 
GCAACAGCTC CGTACGCCAA TCTTAGTGCT CACTTTGCCA GGCTTGTTAA AGGCGGCGGA 
CTGAGGAGTG CTATCGTAAA GATGCTTCTG TATCCGCAGG CTCGCCAGAG CATCGATCTC 
AAAAGGATTG CGTTGCGCAA AAGGCGTTGT CACAAGGCAT CTGCAAAGTT AAAAGATCTC 
AATACCAATC AGGATTCGGG GCAGTCCGAG TTGGAACGAC AGGATGCAAT TGAACTACCA 
ACGGACTCGG AGAGCTATGA CGATGACTTT GAAGATGATT TTTTGCATTC CTGCGACTCG 
GATCCGTTTG AATATGTGCA GTTTTACCAA AACAAGCGGA ACGATTCCAT GTGCAACTCC 
ACAGGCACAT TCGTGGACCA TGGCACGGGT GCCAGGAGGC ACTGTTCCTC TATCAACCTC 
ATTAAATTGG ATTCCGCGGA CACGGACGAA GTTCTAGCGC TCAACGAGTT AAAAAAGGAG 
TCTCTTGTTA TAGGATCCAG AGCACTAAGA GCTTTGGCTC GGAAGTCCAC GACTCGAAAT 
TCCAGTGTGC ACACATCCAC GGCAACTTTG GAGCTAGGTG TGGGTGGCAG CATCACAAAC 
TGTGTTGAGG AAGAGCCAAT TCTTAAGGTC AAGCCATCGT TCTCCCTGCA ACAACAAAGT 
TCCGATGCGA GTTCCATTTT TGCAGCTAGA TTGGGCGGTG CATTCACCGC CTGCGAGAAT 
CTGGCCAGTA TGCCTGATGA TCTCAGCAGG GAGTCGAGTA TCCAAGAGCC ACCCACTCCT 
CTGCCAGCTT CACCGACAGA ACGGCACAGC ATGCCGACCA TATTTGTGGG CAATCGGTTC 
AACCACAGCA AAAATACAGA GGTATATGTA CCGACGTGGC GAGATCGCCA GGAAATGCAG 
AATCAGAGTG TGGATGCGAA GCAGGATGAA GAGTTGCACT CCAGTTCCAT TGATCTACCC 
GCCGCCTGTC TATCTGCTCC GGATAAACTG CAAGCAGAAC TACTCTACAA TTACGACGAG 
ATCCTAGAAA AACCTCTACA GCTGCATCGG GAGCTAACGC CCTTTCCGGG CCATAATCTT 
AACTCGGATA AGCGAGTTTC CCACAAAAGC GACAGTCCGT CGACAGGAAA TGCAAAGACA 
GATCCAAATC TTGCCACAAG AAGTAGTTCC ACCACCGAAC TCTGCATCGA TACCACTTCA 
AAAAAGCGCA CAACCCCGTC AGAGAGAAGC CGGGATTCCA TTAGGCGCTG CATTAGCTAT 
CAGTTCCTGC AGATGTCCAA TCGACCGCCC CCTCCACCAC CGCCACGCAG GGATCCGGAT 
CTACACTTAG ACACCAAATG CCGCTGCTGC GAAAACTCCC AGTGTCCCAG TCCGCGATCG 
AGTGACAGCG GAATGGCTGG CAGTTGCACT ATTACATCAC CGGATCCGCC CAATCCGGAA 
TCATACTTTC CCATGGAGGC CGCAGGCCAC GATATGCTGG ATAATGTGGA GCCAGAGAGG 
TTTGATGTGT GCGGAATGTT CAGAGAGAAG TTCCTCACTC CGGAGGCTAC TCAGGATGTT 
GTTGATCTAT CAGAGGAGCA GCCTCAACTA TCAGATGAAC CCACTACCCC TACCAATCGC 
AAAGAGGAAC CTACTTGCAT CACCTCTGCT CAAGTGCAAG TGAACACAAG GAGTATTTTT 
CTGCCCTCCA GCTCGAGCAT GGATGAGACT AACAGGAATG TCCCAGCCAA CAATATCCTA 
TTTAGCTCGA GTTCCGCGGA TCAGTTGGAA CCGCAGGCCA CCTTTCGCTC GGGGATGTAT 
GCGCACTGGT GGAAGAAAGA GCGCCTTCCG CCGGAGGTGG TGCGTGGCAT AGCCCACGCC 
TACAATAAGA GCTTGCCCTC CAAGGACTCA AAAGATTCGG GATCGGTGTG CTCCAGCTGC 
TTCTGTTCCC TGGGAGCTAG CGGTTACAGC GAGGGCGCAC TCTACTGCTC GGTGTGCCAA 
AACTGTGCGG ACTACTACAA CGGCAGTGTC ACCAGCACAA CCAACACGAC GACCACCACA 
TCATCCGCCA GTTGCCCACT GTGCAGTGAG GACGAGGGCA TGATTGCCCC CACACACGAC 
TCGTCCTCGC TCGACTGTCC CATATGTAAT GGCCGCATTG CTTCCGGCGC CGAAGAAGGC 
AAGCAATCCG AAATGGATCG ACGGCCAGGT AATCTAAGGA TTCCTGTCGA ATGGTATCCG 
GTTTACCTGG CTGGCGGCGT TTGTTCCGTG CACGTGTTAC GTTTGATTGT TTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260623.1
CDS	509..4105
Translation	MDQPLVVQAEYSFMGSNNDELCFQKGDVITVTQREDGGWWEGTLNDKTGWFPSNYVNECKVQLPLTETIRPPEEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQILSQDEYAQLMCNFVEIVRTHEDLLIQIEECNDRVGKLFLTSAPLMKKVHQAYCAAHPKAIVILDKYKDELEKYMERQGAATPGLLVLTTGLSKPFRRLDKYSAMLQELERHMESSHPDRGDTQRSVAVYKDIAATCSATRRQKELELQVLTGPVRGWQGQELSTLGDIIHMGSVAVGADHRDRYFVLFPQTLLFLSVSQRMSAFIYEGKLPLTGIIVNRLEDTDALKNAFEISSPLIDRIVAVCQGPNEANKWVELLNANNPSLPMGIKRQLSNLSNSSLGHLNAAHLSQHLDSRGYCTRFSLCAYYSSPPCHVRPLRVTLPPSNYPATAPYANLSAHFARLVKGGGLRSAIVKMLLYPQARQSIDLKRIALRKRRCHKASAKLKDLNTNQDSGQSELERQDAIELPTDSESYDDDFEDDFLHSCDSDPFEYVQFYQNKRNDSMCNSTGTFVDHGTGARRHCSSINLIKLDSADTDEVLALNELKKESLVIGSRALRALARKSTTRNSSVHTSTATLELGVGGSITNCVEEEPILKVKPSFSLQQQSSDASSIFAARLGGAFTACENLASMPDDLSRESSIQEPPTPLPASPTERHSMPTIFVGNRFNHSKNTEVYVPTWRDRQEMQNQSVDAKQDEELHSSSIDLPAACLSAPDKLQAELLYNYDEILEKPLQLHRELTPFPGHNLNSDKRVSHKSDSPSTGNAKTDPNLATRSSSTTELCIDTTSKKRTTPSERSRDSIRRCISYQFLQMSNRPPPPPPPRRDPDLHLDTKCRCCENSQCPSPRSSDSGMAGSCTITSPDPPNPESYFPMEAAGHDMLDNVEPERFDVCGMFREKFLTPEATQDVVDLSEEQPQLSDEPTTPTNRKEEPTCITSAQVQVNTRSIFLPSSSSMDETNRNVPANNILFSSSSADQLEPQATFRSGMYAHWWKKERLPPEVVRGIAHAYNKSLPSKDSKDSGSVCSSCFCSLGASGYSEGALYCSVCQNCADYYNGSVTSTTNTTTTTSSASCPLCSEDEGMIAPTHDSSSLDCPICNGRIASGAEEGKQSEMDRRPGNLRIPVEWYPVYLAGGVCSVHVLRLIVL

Target ORF information:

RefSeq Version	NM_001273694.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273694.1

ORF Insert Sequence:

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
CTGAGTCAAC ATTTGGATTC ACGGGGCTAC TGCACGCGAT TCTCGCTGTG TGCCTATTAC 
TCCAGCCCCC CGTGCCATGT GCGACCTCTC CGTGTGACTC TTCCCCCAAG CAATTACCCA 
GCAACAGCTC CGTACGCCAA TCTTAGTGCT CACTTTGCCA GGCTTGTTAA AGGCGGCGGA 
CTGAGGAGTG CTATCGTAAA GATGCTTCTG TATCCGCAGG CTCGCCAGAG CATCGATCTC 
AAAAGGATTG CGTTGCGCAA AAGGCGTTGT CACAAGGCAT CTGCAAAGTT AAAAGATCTC 
AATACCAATC AGGATTCGGG GCAGTCCGAG TTGGAACGAC AGGATGCAAT TGAACTACCA 
ACGGACTCGG AGAGCTATGA CGATGACTTT GAAGATGATT TTTTGCATTC CTGCGACTCG 
GATCCGTTTG AATATGTGCA GTTTTACCAA AACAAGCGGA ACGATTCCAT GTGCAACTCC 
ACAGGCACAT TCGTGGACCA TGGCACGGGT GCCAGGAGGC ACTGTTCCTC TATCAACCTC 
ATTAAATTGG ATTCCGCGGA CACGGACGAA GTTCTAGCGC TCAACGAGTT AAAAAAGGAG 
TCTCTTGTTA TAGGATCCAG AGCACTAAGA GCTTTGGCTC GGAAGTCCAC GACTCGAAAT 
TCCAGTGTGC ACACATCCAC GGCAACTTTG GAGCTAGGTG TGGGTGGCAG CATCACAAAC 
TGTGTTGAGG AAGAGCCAAT TCTTAAGGTC AAGCCATCGT TCTCCCTGCA ACAACAAAGT 
TCCGATGCGA GTTCCATTTT TGCAGCTAGA TTGGGCGGTG CATTCACCGC CTGCGAGAAT 
CTGGCCAGTA TGCCTGATGA TCTCAGCAGG GAGTCGAGTA TCCAAGAGCC ACCCACTCCT 
CTGCCAGCTT CACCGACAGA ACGGCACAGC ATGCCGACCA TATTTGTGGG CAATCGGTTC 
AACCACAGCA AAAATACAGA GGTATATGTA CCGACGTGGC GAGATCGCCA GGAAATGCAG 
AATCAGAGTG TGGATGCGAA GCAGGATGAA GAGTTGCACT CCAGTTCCAT TGATCTACCC 
GCCGCCTGTC TATCTGCTCC GGATAAACTG CAAGCAGAAC TACTCTACAA TTACGACGAG 
ATCCTAGAAA AACCTCTACA GCTGCATCGG GAGCTAACGC CCTTTCCGGG CCATAATCTT 
AACTCGGATA AGCGAGTTTC CCACAAAAGC GACAGTCCGT CGACAGGAAA TGCAAAGACA 
GATCCAAATC TTGCCACAAG AAGTAGTTCC ACCACCGAAC TCTGCATCGA TACCACTTCA 
AAAAAGCGCA CAACCCCGTC AGAGAGAAGC CGGGATTCCA TTAGGCGCTG CATTAGCTAT 
CAGTTCCTGC AGATGTCCAA TCGACCGCCC CCTCCACCAC CGCCACGCAG GGATCCGGAT 
CTACACTTAG ACACCAAATG CCGCTGCTGC GAAAACTCCC AGTGTCCCAG TCCGCGATCG 
AGTGACAGCG GAATGGCTGG CAGTTGCACT ATTACATCAC CGGATCCGCC CAATCCGGAA 
TCATACTTTC CCATGGAGGC CGCAGGCCAC GATATGCTGG ATAATGTGGA GCCAGAGAGG 
TTTGATGTGT GCGGAATGTT CAGAGAGAAG TTCCTCACTC CGGAGGCTAC TCAGGATGTT 
GTTGATCTAT CAGAGGAGCA GCCTCAACTA TCAGATGAAC CCACTACCCC TACCAATCGC 
AAAGAGGAAC CTACTTGCAT CACCTCTGCT CAAGTGCAAG TGAACACAAG GAGTATTTTT 
CTGCCCTCCA GCTCGAGCAT GGATGAGACT AACAGGAATG TCCCAGCCAA CAATATCCTA 
TTTAGCTCGA GTTCCGCGGA TCAGTTGGAA CCGCAGGCCA CCTTTCGCTC GGGGATGTAT 
GCGCACTGGT GGAAGAAAGA GCGCCTTCCG CCGGAGGTGG TGCGTGGCAT AGCCCACGCC 
TACAATAAGA GCTTGCCCTC CAAGGACTCA AAAGATTCGG GATCGGTGTG CTCCAGCTGC 
TTCTGTTCCC TGGGAGCTAG CGGTTACAGC GAGGGCGCAC TCTACTGCTC GGTGTGCCAA 
AACTGTGCGG ACTACTACAA CGGCAGTGTC ACCAGCACAA CCAACACGAC GACCACCACA 
TCATCCGCCA GTTGCCCACT GTGCAGTGAG GACGAGGGCA TGATTGCCCC CACACACGAC 
TCGTCCTCGC TCGACTGTCC CATATGTAAT GGCCGCATTG CTTCCGGCGC CGAAGAAGGC 
AAGCAATCCG AAATGGATCG ACGGCCAGGT AATCTAAGGA TTCCTGTCGA ATGGTATCCG 
GTTTACCTGG CTGGCGGCGT TTGTTCCGTG CACGTGTTAC GTTTGATTGT TTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RtGEF ( NM_001103714.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097184.2 Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa16898
Clone ID Related Accession (Same CDS sequence)	NM_001103714.3
Accession Version	NM_001103714.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho-type guanine nucleotide exchange factor, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001103714.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
CTGAGTCAAC ATTTGGATTC ACGGGGCTAC TGCACGCGAT TCTCGCTGTG TGCCTATTAC 
TCCAGCCCCC CGTGCCATGT GCGACCTCTC CGTGTGACTC TTCCCCCAAG CAATTACCCA 
GCAACAGCTC CGTACGCCAA TCTTAGTGCT CACTTTGCCA GGCTTGTTAA AGGCGGCGGA 
CTGAGGAGTG CTATCGTAAA GATGCTTCTG TATCCGCAGG CTCGCCAGAG CATCGATCTC 
AAAAGGATTG CGTTGCGCAA AAGGCGTTGT CACAAGGCAT CTGCAAAGTT AAAAGATCTC 
AATACCAATC AGGATTCGGG GCAGTCCGAG TTGGAACGAC AGGATGCAAT TGAACTACCA 
ACGGACTCGG AGAGCTATGA CGATGACTTT GAAGATGATT TTTTGCATTC CTGCGACTCG 
GATCCGTTTG AATATGTGCA GTTTTACCAA AACAAGCGGA ACGATTCCAT GTGCAACTCC 
ACAGGCACAT TCGTGGACCA TGGCACGGGT GCCAGGAGGC ACTGTTCCTC TATCAACCTC 
ATTAAATTGG ATTCCGCGGA CACGGACGAA GTTCTAGCGC TCAACGAGTT AAAAAAGGAG 
TCTCTTGTTA TAGGATCCAG AGCACTAAGA GCTTTGGCTC GGAAGTCCAC GACTCGAAAT 
TCCAGTGTGC ACACATCCAC GGCAACTTTG GAGCTAGGTG TGGGTGGCAG CATCACAAAC 
TGTGTTGAGG AAGAGCCAAT TCTTAAGGTC AAGCCATCGT TCTCCCTGCA ACAACAAAGT 
TCCGATGCGA GTTCCATTTT TGCAGCTAGA TTGGGCGGTG CATTCACCGC CTGCGAGAAT 
CTGGCCAGTA TGCCTGATGA TCTCAGCAGG GAGTCGAGTA TCCAAGAGCC ACCCACTCCT 
CTGCCAGCTT CACCGACAGA ACGGCACAGC ATGCCGACCA TATTTGTGGG CAATCGGTTC 
AACCACAGCA AAAATACAGA GGTATATGTA CCGACGTGGC GAGATCGCCA GGAAATGCAG 
AATCAGAGTG TGGATGCGAA GCAGGATGAA GAGTTGCACT CCAGTTCCAT TGATCTACCC 
GCCGCCTGTC TATCTGCTCC GGATAAACTG CAAGCAGAAC TACTCTACAA TTACGACGAG 
ATCCTAGAAA AACCTCTACA GCTGCATCGG GAGCTAACGC CCTTTCCGGG CCATAATCTT 
AACTCGGATA AGCGAGTTTC CCACAAAAGC GACAGTCCGT CGACAGGAAA TGCAAAGACA 
GATCCAAATC TTGCCACAAG AAGTAGTTCC ACCACCGAAC TCTGCATCGA TACCACTTCA 
AAAAAGCGCA CAACCCCGTC AGAGAGAAGC CGGGATTCCA TTAGGCGCTG CATTAGCTAT 
CAGTTCCTGC AGATGTCCAA TCGACCGCCC CCTCCACCAC CGCCACGCAG GGATCCGGAT 
CTACACTTAG ACACCAAATG CCGCTGCTGC GAAAACTCCC AGTGTCCCAG TCCGCGATCG 
AGTGACAGCG GAATGGCTGG CAGTTGCACT ATTACATCAC CGGATCCGCC CAATCCGGAA 
TCATACTTTC CCATGGAGGC CGCAGGCCAC GATATGCTGG ATAATGTGGA GCCAGAGAGG 
TTTGATGTGT GCGGAATGTT CAGAGAGAAG TTCCTCACTC CGGAGGCTAC TCAGGATGTT 
GTTGATCTAT CAGAGGAGCA GCCTCAACTA TCAGATGAAC CCACTACCCC TACCAATCGC 
AAAGAGGAAC CTACTTGCAT CACCTCTGCT CAAGTGCAAG TGAACACAAG GAGTATTTTT 
CTGCCCTCCA GCTCGAGCAT GGATGAGACT AACAGGAATG TCCCAGCCAA CAATATCCTA 
TTTAGCTCGA GTTCCGCGGA TCAGTTGGAA CCGCAGGCCA CCTTTCGCTC GGGGATGTAT 
GCGCACTGGT GGAAGAAAGA GCGCCTTCCG CCGGAGGTGG TGCGTGGCAT AGCCCACGCC 
TACAATAAGA GCTTGCCCTC CAAGGACTCA AAAGATTCGG GATCGGTGTG CTCCAGCTGC 
TTCTGTTCCC TGGGAGCTAG CGGTTACAGC GAGGGCGCAC TCTACTGCTC GGTGTGCCAA 
AACTGTGCGG ACTACTACAA CGGCAGTGTC ACCAGCACAA CCAACACGAC GACCACCACA 
TCATCCGCCA GTTGCCCACT GTGCAGTGAG GACGAGGGCA TGATTGCCCC CACACACGAC 
TCGTCCTCGC TCGACTGTCC CATATGTAAT GGCCGCATTG CTTCCGGCGC CGAAGAAGGC 
AAGCAATCCG AAATGGATCG ACGGCCAGAT GTCGCCGCCA TCAAGCCACA TACTCCTACC 
GCCCGGCGGC CGTCCGCCAG CCACTCCCAG CAGCATCCCG GCCGGCAGCA GCAGCGGAGG 
GAGCCACAGC CACCAGGGGT CTCCGGCCAC CAACTCGATG TCGCATCACA AACGGAGCCA 
GTCGTTCAAC CACCATCTCA GCTACAATCA GTACACGCCC ACTCAGCCTC CACCACATCA 
TCTGCATCCA CCACAACCAC CAAGTCTGCC AAGTCATCTG GGGGCATCCG GCCCCAGATC 
AAGTTCAGCC CAGACACCAA ACAGCAAGAC AGCAGCTCCA CTCCAGGCGT CATCCATGGA 
CGCCAGTCCA GCAATTCAGG CAGCGGCAGC AGCAGCGGCG GCAGCGGTGG GAATGGGGGT 
GCCAAGCGCA AGGAAAGGAA GCACAAAGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097184.2
CDS	149..4081
Translation	MDQPLVVQAEYSFMGSNNDELCFQKGDVITVTQREDGGWWEGTLNDKTGWFPSNYVNECKVQLPLTETIRPPEEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQILSQDEYAQLMCNFVEIVRTHEDLLIQIEECNDRVGKLFLTSAPLMKKVHQAYCAAHPKAIVILDKYKDELEKYMERQGAATPGLLVLTTGLSKPFRRLDKYSAMLQELERHMESSHPDRGDTQRSVAVYKDIAATCSATRRQKELELQVLTGPVRGWQGQELSTLGDIIHMGSVAVGADHRDRYFVLFPQTLLFLSVSQRMSAFIYEGKLPLTGIIVNRLEDTDALKNAFEISSPLIDRIVAVCQGPNEANKWVELLNANNPSLPMGIKRQLSNLSNSSLGHLNAAHLSQHLDSRGYCTRFSLCAYYSSPPCHVRPLRVTLPPSNYPATAPYANLSAHFARLVKGGGLRSAIVKMLLYPQARQSIDLKRIALRKRRCHKASAKLKDLNTNQDSGQSELERQDAIELPTDSESYDDDFEDDFLHSCDSDPFEYVQFYQNKRNDSMCNSTGTFVDHGTGARRHCSSINLIKLDSADTDEVLALNELKKESLVIGSRALRALARKSTTRNSSVHTSTATLELGVGGSITNCVEEEPILKVKPSFSLQQQSSDASSIFAARLGGAFTACENLASMPDDLSRESSIQEPPTPLPASPTERHSMPTIFVGNRFNHSKNTEVYVPTWRDRQEMQNQSVDAKQDEELHSSSIDLPAACLSAPDKLQAELLYNYDEILEKPLQLHRELTPFPGHNLNSDKRVSHKSDSPSTGNAKTDPNLATRSSSTTELCIDTTSKKRTTPSERSRDSIRRCISYQFLQMSNRPPPPPPPRRDPDLHLDTKCRCCENSQCPSPRSSDSGMAGSCTITSPDPPNPESYFPMEAAGHDMLDNVEPERFDVCGMFREKFLTPEATQDVVDLSEEQPQLSDEPTTPTNRKEEPTCITSAQVQVNTRSIFLPSSSSMDETNRNVPANNILFSSSSADQLEPQATFRSGMYAHWWKKERLPPEVVRGIAHAYNKSLPSKDSKDSGSVCSSCFCSLGASGYSEGALYCSVCQNCADYYNGSVTSTTNTTTTTSSASCPLCSEDEGMIAPTHDSSSLDCPICNGRIASGAEEGKQSEMDRRPDVAAIKPHTPTARRPSASHSQQHPGRQQQRREPQPPGVSGHQLDVASQTEPVVQPPSQLQSVHAHSASTTSSASTTTTKSAKSSGGIRPQIKFSPDTKQQDSSSTPGVIHGRQSSNSGSGSSSGGSGGNGGAKRKERKHKG

Target ORF information:

RefSeq Version	NM_001103714.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103714.3

ORF Insert Sequence:

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
CTGAGTCAAC ATTTGGATTC ACGGGGCTAC TGCACGCGAT TCTCGCTGTG TGCCTATTAC 
TCCAGCCCCC CGTGCCATGT GCGACCTCTC CGTGTGACTC TTCCCCCAAG CAATTACCCA 
GCAACAGCTC CGTACGCCAA TCTTAGTGCT CACTTTGCCA GGCTTGTTAA AGGCGGCGGA 
CTGAGGAGTG CTATCGTAAA GATGCTTCTG TATCCGCAGG CTCGCCAGAG CATCGATCTC 
AAAAGGATTG CGTTGCGCAA AAGGCGTTGT CACAAGGCAT CTGCAAAGTT AAAAGATCTC 
AATACCAATC AGGATTCGGG GCAGTCCGAG TTGGAACGAC AGGATGCAAT TGAACTACCA 
ACGGACTCGG AGAGCTATGA CGATGACTTT GAAGATGATT TTTTGCATTC CTGCGACTCG 
GATCCGTTTG AATATGTGCA GTTTTACCAA AACAAGCGGA ACGATTCCAT GTGCAACTCC 
ACAGGCACAT TCGTGGACCA TGGCACGGGT GCCAGGAGGC ACTGTTCCTC TATCAACCTC 
ATTAAATTGG ATTCCGCGGA CACGGACGAA GTTCTAGCGC TCAACGAGTT AAAAAAGGAG 
TCTCTTGTTA TAGGATCCAG AGCACTAAGA GCTTTGGCTC GGAAGTCCAC GACTCGAAAT 
TCCAGTGTGC ACACATCCAC GGCAACTTTG GAGCTAGGTG TGGGTGGCAG CATCACAAAC 
TGTGTTGAGG AAGAGCCAAT TCTTAAGGTC AAGCCATCGT TCTCCCTGCA ACAACAAAGT 
TCCGATGCGA GTTCCATTTT TGCAGCTAGA TTGGGCGGTG CATTCACCGC CTGCGAGAAT 
CTGGCCAGTA TGCCTGATGA TCTCAGCAGG GAGTCGAGTA TCCAAGAGCC ACCCACTCCT 
CTGCCAGCTT CACCGACAGA ACGGCACAGC ATGCCGACCA TATTTGTGGG CAATCGGTTC 
AACCACAGCA AAAATACAGA GGTATATGTA CCGACGTGGC GAGATCGCCA GGAAATGCAG 
AATCAGAGTG TGGATGCGAA GCAGGATGAA GAGTTGCACT CCAGTTCCAT TGATCTACCC 
GCCGCCTGTC TATCTGCTCC GGATAAACTG CAAGCAGAAC TACTCTACAA TTACGACGAG 
ATCCTAGAAA AACCTCTACA GCTGCATCGG GAGCTAACGC CCTTTCCGGG CCATAATCTT 
AACTCGGATA AGCGAGTTTC CCACAAAAGC GACAGTCCGT CGACAGGAAA TGCAAAGACA 
GATCCAAATC TTGCCACAAG AAGTAGTTCC ACCACCGAAC TCTGCATCGA TACCACTTCA 
AAAAAGCGCA CAACCCCGTC AGAGAGAAGC CGGGATTCCA TTAGGCGCTG CATTAGCTAT 
CAGTTCCTGC AGATGTCCAA TCGACCGCCC CCTCCACCAC CGCCACGCAG GGATCCGGAT 
CTACACTTAG ACACCAAATG CCGCTGCTGC GAAAACTCCC AGTGTCCCAG TCCGCGATCG 
AGTGACAGCG GAATGGCTGG CAGTTGCACT ATTACATCAC CGGATCCGCC CAATCCGGAA 
TCATACTTTC CCATGGAGGC CGCAGGCCAC GATATGCTGG ATAATGTGGA GCCAGAGAGG 
TTTGATGTGT GCGGAATGTT CAGAGAGAAG TTCCTCACTC CGGAGGCTAC TCAGGATGTT 
GTTGATCTAT CAGAGGAGCA GCCTCAACTA TCAGATGAAC CCACTACCCC TACCAATCGC 
AAAGAGGAAC CTACTTGCAT CACCTCTGCT CAAGTGCAAG TGAACACAAG GAGTATTTTT 
CTGCCCTCCA GCTCGAGCAT GGATGAGACT AACAGGAATG TCCCAGCCAA CAATATCCTA 
TTTAGCTCGA GTTCCGCGGA TCAGTTGGAA CCGCAGGCCA CCTTTCGCTC GGGGATGTAT 
GCGCACTGGT GGAAGAAAGA GCGCCTTCCG CCGGAGGTGG TGCGTGGCAT AGCCCACGCC 
TACAATAAGA GCTTGCCCTC CAAGGACTCA AAAGATTCGG GATCGGTGTG CTCCAGCTGC 
TTCTGTTCCC TGGGAGCTAG CGGTTACAGC GAGGGCGCAC TCTACTGCTC GGTGTGCCAA 
AACTGTGCGG ACTACTACAA CGGCAGTGTC ACCAGCACAA CCAACACGAC GACCACCACA 
TCATCCGCCA GTTGCCCACT GTGCAGTGAG GACGAGGGCA TGATTGCCCC CACACACGAC 
TCGTCCTCGC TCGACTGTCC CATATGTAAT GGCCGCATTG CTTCCGGCGC CGAAGAAGGC 
AAGCAATCCG AAATGGATCG ACGGCCAGAT GTCGCCGCCA TCAAGCCACA TACTCCTACC 
GCCCGGCGGC CGTCCGCCAG CCACTCCCAG CAGCATCCCG GCCGGCAGCA GCAGCGGAGG 
GAGCCACAGC CACCAGGGGT CTCCGGCCAC CAACTCGATG TCGCATCACA AACGGAGCCA 
GTCGTTCAAC CACCATCTCA GCTACAATCA GTACACGCCC ACTCAGCCTC CACCACATCA 
TCTGCATCCA CCACAACCAC CAAGTCTGCC AAGTCATCTG GGGGCATCCG GCCCCAGATC 
AAGTTCAGCC CAGACACCAA ACAGCAAGAC AGCAGCTCCA CTCCAGGCGT CATCCATGGA 
CGCCAGTCCA GCAATTCAGG CAGCGGCAGC AGCAGCGGCG GCAGCGGTGG GAATGGGGGT 
GCCAAGCGCA AGGAAAGGAA GCACAAAGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RtGEF ( NM_001169550.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163021.1 Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant E, mRNA.

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CloneID	OFa15027
Clone ID Related Accession (Same CDS sequence)	NM_001169550.2
Accession Version	NM_001169550.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho-type guanine nucleotide exchange factor, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001169550.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
ATGTCGCCGC CATCAAGCCA CATACTCCTA CCGCCCGGCG GCCGTCCGCC AGCCACTCCC 
AGCAGCATCC CGGCCGGCAG CAGCAGCGGA GGGAGCCACA GCCACCAGGG GTCTCCGGCC 
ACCAACTCGA TGTCGCATCA CAAACGGAGC CAGTCGTTCA ACCACCATCT CAGCTACAAT 
CAGTACACGC CCACTCAGCC TCCACCACAT CATCTGCATC CACCACAACC ACCAAGTCTG 
CCAAGTCATC TGGGGGCATC CGGCCCCAGA TCAAGTTCAG CCCAGACACC AAACAGCAAG 
ACAGCAGCTC CACTCCAGGC GTCATCCATG GACGCCAGTC CAGCAATTCA GGCAGCGGCA 
GCAGCAGCGG CGGCAGCGGT GGGAATGGGG GTGCCAAGCG CAAGGAAAGG AAGCACAAAG 
GCTAACTGGA CCATCAGCTG CCTGCGACCC ACGCCGCCGT TGCGACCCAG TTTATTGAAT 
GCCACCAGCG GATCGGGAAG TGGCAGCGGT GGCAGCGGTG GCGGCGGCAG CAGCTCCAGC 
AATGCCCTGG CCAGCTACTG CAGCGGAAGG AAGAACCAGC CCACCTATGA GGAGGACGCC 
CTGGTGCTCC GGGTTTTCGA GGCCTACTGT GCCGCCTATC AGAACAATGC CAGGAACACC 
ATCCACTCGG GTAAGTGGCG AAGCCCATCC CAGATGTGCT TCATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163021.1
CDS	509..2416
Translation	MDQPLVVQAEYSFMGSNNDELCFQKGDVITVTQREDGGWWEGTLNDKTGWFPSNYVNECKVQLPLTETIRPPEEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQILSQDEYAQLMCNFVEIVRTHEDLLIQIEECNDRVGKLFLTSAPLMKKVHQAYCAAHPKAIVILDKYKDELEKYMERQGAATPGLLVLTTGLSKPFRRLDKYSAMLQELERHMESSHPDRGDTQRSVAVYKDIAATCSATRRQKELELQVLTGPVRGWQGQELSTLGDIIHMGSVAVGADHRDRYFVLFPQTLLFLSVSQRMSAFIYEGKLPLTGIIVNRLEDTDALKNAFEISSPLIDRIVAVCQGPNEANKWVELLNANNPSLPMGIKRQLSNLSNSSLGHLNAAHMSPPSSHILLPPGGRPPATPSSIPAGSSSGGSHSHQGSPATNSMSHHKRSQSFNHHLSYNQYTPTQPPPHHLHPPQPPSLPSHLGASGPRSSSAQTPNSKTAAPLQASSMDASPAIQAAAAAAAAAVGMGVPSARKGSTKANWTISCLRPTPPLRPSLLNATSGSGSGSGGSGGGGSSSSNALASYCSGRKNQPTYEEDALVLRVFEAYCAAYQNNARNTIHSGKWRSPSQMCFI

Target ORF information:

RefSeq Version	NM_001169550.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169550.2

ORF Insert Sequence:

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
ATGTCGCCGC CATCAAGCCA CATACTCCTA CCGCCCGGCG GCCGTCCGCC AGCCACTCCC 
AGCAGCATCC CGGCCGGCAG CAGCAGCGGA GGGAGCCACA GCCACCAGGG GTCTCCGGCC 
ACCAACTCGA TGTCGCATCA CAAACGGAGC CAGTCGTTCA ACCACCATCT CAGCTACAAT 
CAGTACACGC CCACTCAGCC TCCACCACAT CATCTGCATC CACCACAACC ACCAAGTCTG 
CCAAGTCATC TGGGGGCATC CGGCCCCAGA TCAAGTTCAG CCCAGACACC AAACAGCAAG 
ACAGCAGCTC CACTCCAGGC GTCATCCATG GACGCCAGTC CAGCAATTCA GGCAGCGGCA 
GCAGCAGCGG CGGCAGCGGT GGGAATGGGG GTGCCAAGCG CAAGGAAAGG AAGCACAAAG 
GCTAACTGGA CCATCAGCTG CCTGCGACCC ACGCCGCCGT TGCGACCCAG TTTATTGAAT 
GCCACCAGCG GATCGGGAAG TGGCAGCGGT GGCAGCGGTG GCGGCGGCAG CAGCTCCAGC 
AATGCCCTGG CCAGCTACTG CAGCGGAAGG AAGAACCAGC CCACCTATGA GGAGGACGCC 
CTGGTGCTCC GGGTTTTCGA GGCCTACTGT GCCGCCTATC AGAACAATGC CAGGAACACC 
ATCCACTCGG GTAAGTGGCG AAGCCCATCC CAGATGTGCT TCATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RtGEF ( NM_057826.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477174.1 Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant A, mRNA.

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CloneID	OFa15029
Clone ID Related Accession (Same CDS sequence)	NM_057826.5 , NM_165327.3
Accession Version	NM_057826.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho-type guanine nucleotide exchange factor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057826.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
ATGTCGCCGC CATCAAGCCA CATACTCCTA CCGCCCGGCG GCCGTCCGCC AGCCACTCCC 
AGCAGCATCC CGGCCGGCAG CAGCAGCGGA GGGAGCCACA GCCACCAGGG GTCTCCGGCC 
ACCAACTCGA TGTCGCATCA CAAACGGAGC CAGTCGTTCA ACCACCATCT CAGCTACAAT 
CAGTACACGC CCACTCAGCC TCCACCACAT CATCTGCATC CACCACAACC ACCAAGTCTG 
CCAAGTCATC TGGGGGCATC CGGCCCCAGA TCAAGTTCAG CCCAGACACC AAACAGCAAG 
ACAGCAGCTC CACTCCAGGC GTCATCCATG GACGCCAGTC CAGCAATTCA GGCAGCGGCA 
GCAGCAGCGG CGGCAGCGGT GGGAATGGGG GTGCCAAGCG CAAGGAAAGG AAGCACAAAG 
GCTAACTGGA CCATCAGCTG CCTGCGACCC ACGCCGCCGT TGCGACCCAG TTTATTGAAT 
GCCACCAGCG GATCGGGAAG TGGCAGCGGT GGCAGCGGTG GCGGCGGCAG CAGCTCCAGC 
AATGCCCTGG CCAGCTACTG CAGCGGAAGG AAGAACCAGC CCACCTATGA GGAGGACGCC 
CTGGTGCTCC GGGTTTTCGA GGCCTACTGT GCCGCCTATC AGAACAATGC CAGGAACACC 
ATCCACTCGG GCCTTGAAAA CGAGGACATG ACCCCCACCC TGCGCCAACT GTGGACCGCC 
ATCCGCCAGA TGCAGCAGGA CATGTCCCAG ATCAAACTGC AGATCAATGA GGAGCGGGCC 
CTGCGAGCCG ATCTCCAACA GCTGCTCATG CAGCACTTGG AGACGAGCAG TGTGAGCAGC 
GGGGCCAACA CCCCCAAGTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477174.1
CDS	509..2572
Translation	MDQPLVVQAEYSFMGSNNDELCFQKGDVITVTQREDGGWWEGTLNDKTGWFPSNYVNECKVQLPLTETIRPPEEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQILSQDEYAQLMCNFVEIVRTHEDLLIQIEECNDRVGKLFLTSAPLMKKVHQAYCAAHPKAIVILDKYKDELEKYMERQGAATPGLLVLTTGLSKPFRRLDKYSAMLQELERHMESSHPDRGDTQRSVAVYKDIAATCSATRRQKELELQVLTGPVRGWQGQELSTLGDIIHMGSVAVGADHRDRYFVLFPQTLLFLSVSQRMSAFIYEGKLPLTGIIVNRLEDTDALKNAFEISSPLIDRIVAVCQGPNEANKWVELLNANNPSLPMGIKRQLSNLSNSSLGHLNAAHMSPPSSHILLPPGGRPPATPSSIPAGSSSGGSHSHQGSPATNSMSHHKRSQSFNHHLSYNQYTPTQPPPHHLHPPQPPSLPSHLGASGPRSSSAQTPNSKTAAPLQASSMDASPAIQAAAAAAAAAVGMGVPSARKGSTKANWTISCLRPTPPLRPSLLNATSGSGSGSGGSGGGGSSSSNALASYCSGRKNQPTYEEDALVLRVFEAYCAAYQNNARNTIHSGLENEDMTPTLRQLWTAIRQMQQDMSQIKLQINEERALRADLQQLLMQHLETSSVSSGANTPKC

Target ORF information:

RefSeq Version	NM_057826.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057826.5

ORF Insert Sequence:

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
ATGTCGCCGC CATCAAGCCA CATACTCCTA CCGCCCGGCG GCCGTCCGCC AGCCACTCCC 
AGCAGCATCC CGGCCGGCAG CAGCAGCGGA GGGAGCCACA GCCACCAGGG GTCTCCGGCC 
ACCAACTCGA TGTCGCATCA CAAACGGAGC CAGTCGTTCA ACCACCATCT CAGCTACAAT 
CAGTACACGC CCACTCAGCC TCCACCACAT CATCTGCATC CACCACAACC ACCAAGTCTG 
CCAAGTCATC TGGGGGCATC CGGCCCCAGA TCAAGTTCAG CCCAGACACC AAACAGCAAG 
ACAGCAGCTC CACTCCAGGC GTCATCCATG GACGCCAGTC CAGCAATTCA GGCAGCGGCA 
GCAGCAGCGG CGGCAGCGGT GGGAATGGGG GTGCCAAGCG CAAGGAAAGG AAGCACAAAG 
GCTAACTGGA CCATCAGCTG CCTGCGACCC ACGCCGCCGT TGCGACCCAG TTTATTGAAT 
GCCACCAGCG GATCGGGAAG TGGCAGCGGT GGCAGCGGTG GCGGCGGCAG CAGCTCCAGC 
AATGCCCTGG CCAGCTACTG CAGCGGAAGG AAGAACCAGC CCACCTATGA GGAGGACGCC 
CTGGTGCTCC GGGTTTTCGA GGCCTACTGT GCCGCCTATC AGAACAATGC CAGGAACACC 
ATCCACTCGG GCCTTGAAAA CGAGGACATG ACCCCCACCC TGCGCCAACT GTGGACCGCC 
ATCCGCCAGA TGCAGCAGGA CATGTCCCAG ATCAAACTGC AGATCAATGA GGAGCGGGCC 
CTGCGAGCCG ATCTCCAACA GCTGCTCATG CAGCACTTGG AGACGAGCAG TGTGAGCAGC 
GGGGCCAACA CCCCCAAGTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RtGEF ( NM_165327.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724258.1 Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant B, mRNA.

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CloneID	OFa15029
Clone ID Related Accession (Same CDS sequence)	NM_057826.5 , NM_165327.3
Accession Version	NM_165327.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho-type guanine nucleotide exchange factor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165327.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
ATGTCGCCGC CATCAAGCCA CATACTCCTA CCGCCCGGCG GCCGTCCGCC AGCCACTCCC 
AGCAGCATCC CGGCCGGCAG CAGCAGCGGA GGGAGCCACA GCCACCAGGG GTCTCCGGCC 
ACCAACTCGA TGTCGCATCA CAAACGGAGC CAGTCGTTCA ACCACCATCT CAGCTACAAT 
CAGTACACGC CCACTCAGCC TCCACCACAT CATCTGCATC CACCACAACC ACCAAGTCTG 
CCAAGTCATC TGGGGGCATC CGGCCCCAGA TCAAGTTCAG CCCAGACACC AAACAGCAAG 
ACAGCAGCTC CACTCCAGGC GTCATCCATG GACGCCAGTC CAGCAATTCA GGCAGCGGCA 
GCAGCAGCGG CGGCAGCGGT GGGAATGGGG GTGCCAAGCG CAAGGAAAGG AAGCACAAAG 
GCTAACTGGA CCATCAGCTG CCTGCGACCC ACGCCGCCGT TGCGACCCAG TTTATTGAAT 
GCCACCAGCG GATCGGGAAG TGGCAGCGGT GGCAGCGGTG GCGGCGGCAG CAGCTCCAGC 
AATGCCCTGG CCAGCTACTG CAGCGGAAGG AAGAACCAGC CCACCTATGA GGAGGACGCC 
CTGGTGCTCC GGGTTTTCGA GGCCTACTGT GCCGCCTATC AGAACAATGC CAGGAACACC 
ATCCACTCGG GCCTTGAAAA CGAGGACATG ACCCCCACCC TGCGCCAACT GTGGACCGCC 
ATCCGCCAGA TGCAGCAGGA CATGTCCCAG ATCAAACTGC AGATCAATGA GGAGCGGGCC 
CTGCGAGCCG ATCTCCAACA GCTGCTCATG CAGCACTTGG AGACGAGCAG TGTGAGCAGC 
GGGGCCAACA CCCCCAAGTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724258.1
CDS	149..2212
Translation	MDQPLVVQAEYSFMGSNNDELCFQKGDVITVTQREDGGWWEGTLNDKTGWFPSNYVNECKVQLPLTETIRPPEEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQILSQDEYAQLMCNFVEIVRTHEDLLIQIEECNDRVGKLFLTSAPLMKKVHQAYCAAHPKAIVILDKYKDELEKYMERQGAATPGLLVLTTGLSKPFRRLDKYSAMLQELERHMESSHPDRGDTQRSVAVYKDIAATCSATRRQKELELQVLTGPVRGWQGQELSTLGDIIHMGSVAVGADHRDRYFVLFPQTLLFLSVSQRMSAFIYEGKLPLTGIIVNRLEDTDALKNAFEISSPLIDRIVAVCQGPNEANKWVELLNANNPSLPMGIKRQLSNLSNSSLGHLNAAHMSPPSSHILLPPGGRPPATPSSIPAGSSSGGSHSHQGSPATNSMSHHKRSQSFNHHLSYNQYTPTQPPPHHLHPPQPPSLPSHLGASGPRSSSAQTPNSKTAAPLQASSMDASPAIQAAAAAAAAAVGMGVPSARKGSTKANWTISCLRPTPPLRPSLLNATSGSGSGSGGSGGGGSSSSNALASYCSGRKNQPTYEEDALVLRVFEAYCAAYQNNARNTIHSGLENEDMTPTLRQLWTAIRQMQQDMSQIKLQINEERALRADLQQLLMQHLETSSVSSGANTPKC

Target ORF information:

RefSeq Version	NM_165327.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165327.3

ORF Insert Sequence:

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
ATGTCGCCGC CATCAAGCCA CATACTCCTA CCGCCCGGCG GCCGTCCGCC AGCCACTCCC 
AGCAGCATCC CGGCCGGCAG CAGCAGCGGA GGGAGCCACA GCCACCAGGG GTCTCCGGCC 
ACCAACTCGA TGTCGCATCA CAAACGGAGC CAGTCGTTCA ACCACCATCT CAGCTACAAT 
CAGTACACGC CCACTCAGCC TCCACCACAT CATCTGCATC CACCACAACC ACCAAGTCTG 
CCAAGTCATC TGGGGGCATC CGGCCCCAGA TCAAGTTCAG CCCAGACACC AAACAGCAAG 
ACAGCAGCTC CACTCCAGGC GTCATCCATG GACGCCAGTC CAGCAATTCA GGCAGCGGCA 
GCAGCAGCGG CGGCAGCGGT GGGAATGGGG GTGCCAAGCG CAAGGAAAGG AAGCACAAAG 
GCTAACTGGA CCATCAGCTG CCTGCGACCC ACGCCGCCGT TGCGACCCAG TTTATTGAAT 
GCCACCAGCG GATCGGGAAG TGGCAGCGGT GGCAGCGGTG GCGGCGGCAG CAGCTCCAGC 
AATGCCCTGG CCAGCTACTG CAGCGGAAGG AAGAACCAGC CCACCTATGA GGAGGACGCC 
CTGGTGCTCC GGGTTTTCGA GGCCTACTGT GCCGCCTATC AGAACAATGC CAGGAACACC 
ATCCACTCGG GCCTTGAAAA CGAGGACATG ACCCCCACCC TGCGCCAACT GTGGACCGCC 
ATCCGCCAGA TGCAGCAGGA CATGTCCCAG ATCAAACTGC AGATCAATGA GGAGCGGGCC 
CTGCGAGCCG ATCTCCAACA GCTGCTCATG CAGCACTTGG AGACGAGCAG TGTGAGCAGC 
GGGGCCAACA CCCCCAAGTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RtGEF ( NM_001103715.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097185.2 Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant D, mRNA.

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CloneID	OFa16939
Clone ID Related Accession (Same CDS sequence)	NM_001103715.4
Accession Version	NM_001103715.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho-type guanine nucleotide exchange factor, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001103715.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
ATGTCGCCGC CATCAAGCCA CATACTCCTA CCGCCCGGCG GCCGTCCGCC AGCCACTCCC 
AGCAGCATCC CGGCCGGCAG CAGCAGCGGA GGGAGCCACA GCCACCAGGG GTCTCCGGCC 
ACCAACTCGA TGTCGCATCA CAAACGGAGC CAGTCGTTCA ACCACCATCT CAGCTACAAT 
CAGTACACGC CCACTCAGCC TCCACCACAT CATCTGCATC CACCACAACC ACCAAGTCTG 
CCAAGTCATC TGGGGGCATC CGGCCCCAGA TCAAGTTCAG CCCAGACACC AAACAGCAAG 
ACAGCAGCTC CACTCCAGGC GTCATCCATG GACGCCAGTC CAGCAATTCA GGCAGCGGCA 
GCAGCAGCGG CGGCAGCGGT GGGAATGGGG GTGCCAAGCG CAAGGAAAGG AAGCACAAAG 
GCTAACTGGA CCATCAGCTG CCTGCGACCC ACGCCGCCGT TGCGACCCAG TTTATTGAAT 
GCCACCAGCG GATCGGGAAG TGGCAGCGGT GGCAGCGGTG GCGGCGGCAG CAGCTCCAGC 
AATGCCCTGG CCAGCTACTG CAGCGGAAGG AAGAACCAGC CCACCTATGA GGAGGACGCC 
CTGGTGCTCC GGGTTTTCGA GGCCTACTGT GCCGCCTATC AGAACAATGC CAGGAACACC 
ATCCACTCGG CCTTGCAACC CTGGACCCCG TGTCTGCCCA TCCGCGGTGG CAAGCTGAAA 
ACGAGCAAAA CCTTCTCGAC CTCCAATCCA CAGCTGCCTT TCATAGCCAA TGTCGGCAAC 
TCAGCCTACC TCAATCAGCA CCAGCAGCAG CATCTGCAGC AGCAGCACCT GCAGCAGCAA 
CACTTGCAGC AGCAACATCT TGCCCAGCAG CAACCAGGTC GTCAGCACTC CGCCGGCAGT 
TTGAACTCCT CATTTATTTG GCGCGAGGCG AGCCCGAACA ACTTCAACCT ATACTCCAGT 
TCGGTGTCCT CCTCGCTGAA TGCGACACCT GCCCAACGCT ACTCCTTATC CGGAACTGCG 
GGAGGATCGA CGTCCATCAA GGACTACCAG AGAGCGCTCT CCGAGGAGAG GGCAACCAGA 
AAGTCTCTGA ATCGCCTTAA AAGTGGCTTT GGTTCGGGCT ACGACAATGT GTGTACATCA 
CCTCTGCTGC CGAGGAAAAA CAAACCGGCA CCAAAGTTGG TGGCCCAGCA GTCGTATCAG 
AGATCCCCGG GAAACGCCAC AGCCACCACT ATAGTGAAGC CGAATCCGCC AGGACACCCA 
GGACTTTACG ATCGCCGCTT GAACCGCTCC TTTGAGACCT CCACTTCGCA CCGCTATGCC 
GGGTATGGAG CGGGCTACTT GATGAATTGC GGCGGAGCCT TGCGGACCAG TACGCTCCAG 
AGGAGCACTC CGCAACTGGC CGGTGGTGAA AGATCCGATG ACAGCGATGT GGAGCGGGAA 
CTGCTGCGGG GTAATGGAGC TGGGCCCACG GCCAGTTTGG AACTTAATCC CGATGGCAGC 
AAACGGCAGT CGGGCAGCTG GTGTTGCGGC CTTGAAAACG AGGACATGAC CCCCACCCTG 
CGCCAACTGT GGACCGCCAT CCGCCAGATG CAGCAGGACA TGTCCCAGAT CAAACTGCAG 
ATCAATGAGG AGCGGGCCCT GCGAGCCGAT CTCCAACAGC TGCTCATGCA GCACTTGGAG 
ACGAGCAGTG TGAGCAGCGG GGCCAACACC CCCAAGTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097185.2
CDS	509..3430
Translation	MDQPLVVQAEYSFMGSNNDELCFQKGDVITVTQREDGGWWEGTLNDKTGWFPSNYVNECKVQLPLTETIRPPEEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQILSQDEYAQLMCNFVEIVRTHEDLLIQIEECNDRVGKLFLTSAPLMKKVHQAYCAAHPKAIVILDKYKDELEKYMERQGAATPGLLVLTTGLSKPFRRLDKYSAMLQELERHMESSHPDRGDTQRSVAVYKDIAATCSATRRQKELELQVLTGPVRGWQGQELSTLGDIIHMGSVAVGADHRDRYFVLFPQTLLFLSVSQRMSAFIYEGKLPLTGIIVNRLEDTDALKNAFEISSPLIDRIVAVCQGPNEANKWVELLNANNPSLPMGIKRQLSNLSNSSLGHLNAAHMSPPSSHILLPPGGRPPATPSSIPAGSSSGGSHSHQGSPATNSMSHHKRSQSFNHHLSYNQYTPTQPPPHHLHPPQPPSLPSHLGASGPRSSSAQTPNSKTAAPLQASSMDASPAIQAAAAAAAAAVGMGVPSARKGSTKANWTISCLRPTPPLRPSLLNATSGSGSGSGGSGGGGSSSSNALASYCSGRKNQPTYEEDALVLRVFEAYCAAYQNNARNTIHSALQPWTPCLPIRGGKLKTSKTFSTSNPQLPFIANVGNSAYLNQHQQQHLQQQHLQQQHLQQQHLAQQQPGRQHSAGSLNSSFIWREASPNNFNLYSSSVSSSLNATPAQRYSLSGTAGGSTSIKDYQRALSEERATRKSLNRLKSGFGSGYDNVCTSPLLPRKNKPAPKLVAQQSYQRSPGNATATTIVKPNPPGHPGLYDRRLNRSFETSTSHRYAGYGAGYLMNCGGALRTSTLQRSTPQLAGGERSDDSDVERELLRGNGAGPTASLELNPDGSKRQSGSWCCGLENEDMTPTLRQLWTAIRQMQQDMSQIKLQINEERALRADLQQLLMQHLETSSVSSGANTPKC

Target ORF information:

RefSeq Version	NM_001103715.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103715.4

ORF Insert Sequence:

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
ATGTCGCCGC CATCAAGCCA CATACTCCTA CCGCCCGGCG GCCGTCCGCC AGCCACTCCC 
AGCAGCATCC CGGCCGGCAG CAGCAGCGGA GGGAGCCACA GCCACCAGGG GTCTCCGGCC 
ACCAACTCGA TGTCGCATCA CAAACGGAGC CAGTCGTTCA ACCACCATCT CAGCTACAAT 
CAGTACACGC CCACTCAGCC TCCACCACAT CATCTGCATC CACCACAACC ACCAAGTCTG 
CCAAGTCATC TGGGGGCATC CGGCCCCAGA TCAAGTTCAG CCCAGACACC AAACAGCAAG 
ACAGCAGCTC CACTCCAGGC GTCATCCATG GACGCCAGTC CAGCAATTCA GGCAGCGGCA 
GCAGCAGCGG CGGCAGCGGT GGGAATGGGG GTGCCAAGCG CAAGGAAAGG AAGCACAAAG 
GCTAACTGGA CCATCAGCTG CCTGCGACCC ACGCCGCCGT TGCGACCCAG TTTATTGAAT 
GCCACCAGCG GATCGGGAAG TGGCAGCGGT GGCAGCGGTG GCGGCGGCAG CAGCTCCAGC 
AATGCCCTGG CCAGCTACTG CAGCGGAAGG AAGAACCAGC CCACCTATGA GGAGGACGCC 
CTGGTGCTCC GGGTTTTCGA GGCCTACTGT GCCGCCTATC AGAACAATGC CAGGAACACC 
ATCCACTCGG CCTTGCAACC CTGGACCCCG TGTCTGCCCA TCCGCGGTGG CAAGCTGAAA 
ACGAGCAAAA CCTTCTCGAC CTCCAATCCA CAGCTGCCTT TCATAGCCAA TGTCGGCAAC 
TCAGCCTACC TCAATCAGCA CCAGCAGCAG CATCTGCAGC AGCAGCACCT GCAGCAGCAA 
CACTTGCAGC AGCAACATCT TGCCCAGCAG CAACCAGGTC GTCAGCACTC CGCCGGCAGT 
TTGAACTCCT CATTTATTTG GCGCGAGGCG AGCCCGAACA ACTTCAACCT ATACTCCAGT 
TCGGTGTCCT CCTCGCTGAA TGCGACACCT GCCCAACGCT ACTCCTTATC CGGAACTGCG 
GGAGGATCGA CGTCCATCAA GGACTACCAG AGAGCGCTCT CCGAGGAGAG GGCAACCAGA 
AAGTCTCTGA ATCGCCTTAA AAGTGGCTTT GGTTCGGGCT ACGACAATGT GTGTACATCA 
CCTCTGCTGC CGAGGAAAAA CAAACCGGCA CCAAAGTTGG TGGCCCAGCA GTCGTATCAG 
AGATCCCCGG GAAACGCCAC AGCCACCACT ATAGTGAAGC CGAATCCGCC AGGACACCCA 
GGACTTTACG ATCGCCGCTT GAACCGCTCC TTTGAGACCT CCACTTCGCA CCGCTATGCC 
GGGTATGGAG CGGGCTACTT GATGAATTGC GGCGGAGCCT TGCGGACCAG TACGCTCCAG 
AGGAGCACTC CGCAACTGGC CGGTGGTGAA AGATCCGATG ACAGCGATGT GGAGCGGGAA 
CTGCTGCGGG GTAATGGAGC TGGGCCCACG GCCAGTTTGG AACTTAATCC CGATGGCAGC 
AAACGGCAGT CGGGCAGCTG GTGTTGCGGC CTTGAAAACG AGGACATGAC CCCCACCCTG 
CGCCAACTGT GGACCGCCAT CCGCCAGATG CAGCAGGACA TGTCCCAGAT CAAACTGCAG 
ATCAATGAGG AGCGGGCCCT GCGAGCCGAT CTCCAACAGC TGCTCATGCA GCACTTGGAG 
ACGAGCAGTG TGAGCAGCGG GGCCAACACC CCCAAGTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RtGEF ( NM_001273693.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260622.1 Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant G, mRNA.

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CloneID	OFa16944
Clone ID Related Accession (Same CDS sequence)	NM_001273693.1
Accession Version	NM_001273693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho-type guanine nucleotide exchange factor, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
ATGTCGCCGC CATCAAGCCA CATACTCCTA CCGCCCGGCG GCCGTCCGCC AGCCACTCCC 
AGCAGCATCC CGGCCGGCAG CAGCAGCGGA GGGAGCCACA GCCACCAGGG GTCTCCGGCC 
ACCAACTCGA TGTCGCATCA CAAACGGAGC CAGTCGTTCA ACCACCATCT CAGCTACAAT 
CAGTACACGC CCACTCAGCC TCCACCACAT CATCTGCATC CACCACAACC ACCAAGTCTG 
CCAAGTCATC TGGGGGCATC CGGCCCCAGA TCAAGTTCAG CCCAGACACC AAACAGCAAG 
ACAGCAGCTC CACTCCAGGC GTCATCCATG GACGCCAGTC CAGCAATTCA GGCAGCGGCA 
GCAGCAGCGG CGGCAGCGGT GGGAATGGGG GTGCCAAGCG CAAGGAAAGG AAGCACAAAG 
GCTAACTGGA CCATCAGCTG CCTGCGACCC ACGCCGCCGT TGCGACCCAG TTTATTGAAT 
GCCACCAGCG GATCGGGAAG TGGCAGCGGT GGCAGCGGTG GCGGCGGCAG CAGCTCCAGC 
AATGCCCTGG CCAGCTACTG CAGCGGAAGG AAGAACCAGC CCACCTATGA GGAGGACGCC 
CTGGTGCTCC GGGTTTTCGA GGCCTACTGT GCCGCCTATC AGAACAATGC CAGGAACACC 
ATCCACTCGG CCTTGCAACC CTGGACCCCG TGTCTGCCCA TCCGCGGTGG CAAGCTGAAA 
ACGAGCAAAA CCTTCTCGAC CTCCAATCCA CAGCTGCCTT TCATAGCCAA TGTCGGCAAC 
TCAGCCTACC TCAATCAGCA CCAGCAGCAG CATCTGCAGC AGCAGCACCT GCAGCAGCAA 
CACTTGCAGC AGCAACATCT TGCCCAGCAG CAACCAGGTC GTCAGCACTC CGCCGGCAGT 
TTGAACTCCT CATTTATTTG GCGCGAGGCG AGCCCGAACA ACTTCAACCT ATACTCCAGT 
TCGGTGTCCT CCTCGCTGAA TGCGACACCT GCCCAACGCT ACTCCTTATC CGGAACTGCG 
GGAGGATCGA CGTCCATCAA GGACTACCAG AGAGCGCTCT CCGAGGAGAG GGCAACCAGA 
AAGTCTCTGA ATCGCCTTAA AAGTGGCTTT GGTTCGGGCT ACGACAATGT GTGTACATCA 
CCTCTGCTGC CGAGGAAAAA CAAACCGGCA CCAAAGTTGG TGGCCCAGCA GTCGTATCAG 
AGATCCCCGG GAAACGCCAC AGCCACCACT ATAGTGAAGC CGAATCCGCC AGGACACCCA 
GGACTTTACG ATCGCCGCTT GAACCGCTCC TTTGAGACCT CCACTTCGCA CCGCTATGCC 
GGGTATGGAG CGGGCTACTT GATGAATTGC GGCGGAGCCT TGCGGACCAG TACGCTCCAG 
AGGAGCACTC CGCAACTGGC CGGTGGTGAA AGATCCGATG ACAGCGATGT GGAGCGGGAA 
CTGCTGCGGG GTAATGGAGC TGGGCCCACG GCCAGTTTGG AACTTAATCC CGATGGCAGC 
AAACGGCAGT CGGGCAGCTG GTGTTGCGGT AATTTTGTGA TGAAGCAGTG GCGCAAGATC 
AACCATGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260622.1
CDS	149..2920
Translation	MDQPLVVQAEYSFMGSNNDELCFQKGDVITVTQREDGGWWEGTLNDKTGWFPSNYVNECKVQLPLTETIRPPEEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQILSQDEYAQLMCNFVEIVRTHEDLLIQIEECNDRVGKLFLTSAPLMKKVHQAYCAAHPKAIVILDKYKDELEKYMERQGAATPGLLVLTTGLSKPFRRLDKYSAMLQELERHMESSHPDRGDTQRSVAVYKDIAATCSATRRQKELELQVLTGPVRGWQGQELSTLGDIIHMGSVAVGADHRDRYFVLFPQTLLFLSVSQRMSAFIYEGKLPLTGIIVNRLEDTDALKNAFEISSPLIDRIVAVCQGPNEANKWVELLNANNPSLPMGIKRQLSNLSNSSLGHLNAAHMSPPSSHILLPPGGRPPATPSSIPAGSSSGGSHSHQGSPATNSMSHHKRSQSFNHHLSYNQYTPTQPPPHHLHPPQPPSLPSHLGASGPRSSSAQTPNSKTAAPLQASSMDASPAIQAAAAAAAAAVGMGVPSARKGSTKANWTISCLRPTPPLRPSLLNATSGSGSGSGGSGGGGSSSSNALASYCSGRKNQPTYEEDALVLRVFEAYCAAYQNNARNTIHSALQPWTPCLPIRGGKLKTSKTFSTSNPQLPFIANVGNSAYLNQHQQQHLQQQHLQQQHLQQQHLAQQQPGRQHSAGSLNSSFIWREASPNNFNLYSSSVSSSLNATPAQRYSLSGTAGGSTSIKDYQRALSEERATRKSLNRLKSGFGSGYDNVCTSPLLPRKNKPAPKLVAQQSYQRSPGNATATTIVKPNPPGHPGLYDRRLNRSFETSTSHRYAGYGAGYLMNCGGALRTSTLQRSTPQLAGGERSDDSDVERELLRGNGAGPTASLELNPDGSKRQSGSWCCGNFVMKQWRKINHV

Target ORF information:

RefSeq Version	NM_001273693.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho-type guanine nucleotide exchange factor (RtGEF), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273693.1

ORF Insert Sequence:

ATGGATCAGC CACTGGTGGT GCAGGCGGAG TACTCCTTCA TGGGCAGCAA TAACGACGAG 
CTGTGCTTCC AGAAGGGCGA TGTCATCACG GTTACCCAGC GCGAGGATGG TGGCTGGTGG 
GAGGGAACGC TGAACGATAA GACTGGCTGG TTTCCCAGCA ACTATGTGAA TGAGTGCAAG 
GTGCAGCTGC CTCTAACGGA GACCATAAGG CCGCCGGAGG AGATCCAGGA GTATCGGTCT 
GTGGTGCTCA AGGATCTGCT TGACTCGGAG CGCGCCCATG TGGCCGAGCT GCAGGGTCTG 
CTCGAGAATT TCTTGGAGCC CATGCAACAG ACGCAAATAC TCAGTCAGGA TGAGTACGCC 
CAGCTGATGT GTAACTTTGT AGAGATTGTG CGTACGCACG AGGATTTGCT TATCCAGATC 
GAGGAGTGCA ACGATCGAGT GGGAAAACTA TTTCTCACCA GCGCCCCCTT GATGAAGAAG 
GTGCACCAGG CGTACTGCGC TGCCCATCCA AAGGCCATTG TCATACTGGA TAAGTACAAG 
GACGAGCTGG AAAAGTATAT GGAACGACAG GGCGCAGCCA CTCCTGGATT ACTTGTGCTC 
ACGACGGGTC TATCAAAGCC CTTCCGGCGA CTGGACAAAT ACTCGGCCAT GCTGCAGGAG 
CTGGAGCGGC ATATGGAGAG CAGTCATCCG GATCGCGGCG ACACCCAGCG GAGTGTTGCC 
GTGTACAAAG ACATAGCTGC CACCTGCTCG GCCACCCGTC GCCAAAAGGA GCTGGAGCTG 
CAAGTGCTCA CGGGGCCAGT GCGTGGATGG CAGGGCCAAG AGCTGAGCAC CCTCGGGGAC 
ATCATCCACA TGGGCAGCGT TGCAGTTGGA GCTGATCATC GCGACCGATA CTTTGTGCTC 
TTCCCTCAAA CATTGCTATT CCTTAGCGTT AGTCAGCGGA TGAGTGCATT CATCTATGAG 
GGCAAGCTAC CCTTAACTGG GATCATAGTG AATCGCCTGG AGGACACGGA TGCCCTGAAG 
AACGCCTTTG AGATAAGCAG TCCCTTGATC GATCGCATTG TAGCAGTTTG TCAGGGTCCG 
AATGAGGCCA ACAAGTGGGT GGAGCTGCTC AATGCCAATA ATCCCAGCTT GCCGATGGGC 
ATCAAACGGC AATTGAGTAA CTTGAGCAAC TCTTCGCTAG GACACCTAAA TGCCGCTCAT 
ATGTCGCCGC CATCAAGCCA CATACTCCTA CCGCCCGGCG GCCGTCCGCC AGCCACTCCC 
AGCAGCATCC CGGCCGGCAG CAGCAGCGGA GGGAGCCACA GCCACCAGGG GTCTCCGGCC 
ACCAACTCGA TGTCGCATCA CAAACGGAGC CAGTCGTTCA ACCACCATCT CAGCTACAAT 
CAGTACACGC CCACTCAGCC TCCACCACAT CATCTGCATC CACCACAACC ACCAAGTCTG 
CCAAGTCATC TGGGGGCATC CGGCCCCAGA TCAAGTTCAG CCCAGACACC AAACAGCAAG 
ACAGCAGCTC CACTCCAGGC GTCATCCATG GACGCCAGTC CAGCAATTCA GGCAGCGGCA 
GCAGCAGCGG CGGCAGCGGT GGGAATGGGG GTGCCAAGCG CAAGGAAAGG AAGCACAAAG 
GCTAACTGGA CCATCAGCTG CCTGCGACCC ACGCCGCCGT TGCGACCCAG TTTATTGAAT 
GCCACCAGCG GATCGGGAAG TGGCAGCGGT GGCAGCGGTG GCGGCGGCAG CAGCTCCAGC 
AATGCCCTGG CCAGCTACTG CAGCGGAAGG AAGAACCAGC CCACCTATGA GGAGGACGCC 
CTGGTGCTCC GGGTTTTCGA GGCCTACTGT GCCGCCTATC AGAACAATGC CAGGAACACC 
ATCCACTCGG CCTTGCAACC CTGGACCCCG TGTCTGCCCA TCCGCGGTGG CAAGCTGAAA 
ACGAGCAAAA CCTTCTCGAC CTCCAATCCA CAGCTGCCTT TCATAGCCAA TGTCGGCAAC 
TCAGCCTACC TCAATCAGCA CCAGCAGCAG CATCTGCAGC AGCAGCACCT GCAGCAGCAA 
CACTTGCAGC AGCAACATCT TGCCCAGCAG CAACCAGGTC GTCAGCACTC CGCCGGCAGT 
TTGAACTCCT CATTTATTTG GCGCGAGGCG AGCCCGAACA ACTTCAACCT ATACTCCAGT 
TCGGTGTCCT CCTCGCTGAA TGCGACACCT GCCCAACGCT ACTCCTTATC CGGAACTGCG 
GGAGGATCGA CGTCCATCAA GGACTACCAG AGAGCGCTCT CCGAGGAGAG GGCAACCAGA 
AAGTCTCTGA ATCGCCTTAA AAGTGGCTTT GGTTCGGGCT ACGACAATGT GTGTACATCA 
CCTCTGCTGC CGAGGAAAAA CAAACCGGCA CCAAAGTTGG TGGCCCAGCA GTCGTATCAG 
AGATCCCCGG GAAACGCCAC AGCCACCACT ATAGTGAAGC CGAATCCGCC AGGACACCCA 
GGACTTTACG ATCGCCGCTT GAACCGCTCC TTTGAGACCT CCACTTCGCA CCGCTATGCC 
GGGTATGGAG CGGGCTACTT GATGAATTGC GGCGGAGCCT TGCGGACCAG TACGCTCCAG 
AGGAGCACTC CGCAACTGGC CGGTGGTGAA AGATCCGATG ACAGCGATGT GGAGCGGGAA 
CTGCTGCGGG GTAATGGAGC TGGGCCCACG GCCAGTTTGG AACTTAATCC CGATGGCAGC 
AAACGGCAGT CGGGCAGCTG GTGTTGCGGT AATTTTGTGA TGAAGCAGTG GCGCAAGATC 
AACCATGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RtGEF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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