lok cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	lok
Entrez Gene ID	35288
Full Name	loki
Synonyms	10895,38B.4,CG10895,CHK-2,CHK2,Chk2,DmCHk2,DmChk2,Dmchk2,Dmel\CG10895,Dmnk,Dmnk-L,Dmnk-S,Dmnk/DChk2,LOKI/CHK2,MNK,chk,chk2,chk2/lok,mnk
General protein information	Preferred Names loki Names CG10895 gene product from transcript CG10895-RA CG10895-PA CG10895-PB CG10895-PC CG10895-PD checkpoint kinase 2 drosophila maternal nuclear kinase localized ovarian kinase lok-PA lok-PB lok-PC lok-PD maternal nuclear kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38B2-38B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259171.2	NP_001246100.1	loki, isoform D
NM_057870.4	NP_477218.1	loki, isoform B
NM_165318.3	NP_724241.1	loki, isoform C
NM_057871.4	NP_477219.1	loki, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHEK2	NP_001005735.1
Rattus norvegicus (Norway rat)	Chek2	NP_446129.1
Saccharomyces cerevisiae (baker's yeast)	RAD53	NP_015172.1
Bos taurus (cattle)	CHEK2	NP_001029703.1
Gallus gallus (chicken)	CHEK2	NP_001073576.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009784	XP_318879.3
Caenorhabditis elegans (roundworm)	CELE_T08D2.7	NP_508060.1
Caenorhabditis elegans (roundworm)	chk-2	NP_001024271.1
Kluyveromyces lactis	KLLA0F11143g	XP_455587.1
	CHEK2	XP_001101658.1
Mus musculus (house mouse)	Chek2	NP_057890.1
Danio rerio (zebrafish)	chek2	NP_956339.1
Pan troglodytes (chimpanzee)	CHEK2	XP_003317205.1
Canis lupus familiaris (dog)	CHEK2	XP_005636447.1
Drosophila melanogaster (fruit fly)	lok	NP_724241.1
Eremothecium gossypii	AGOS_ACR142W	NP_983544.2
Xenopus tropicalis (tropical clawed frog)	chek2	NP_001119996.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study demonstrates, that Lok kinase activation is required for the DNA damage-mediated disruption of adult stem cell self-renewal and lineage differentiation, and might also offer novel insight into how DNA damage causes tissue aging and cancer formation.
Title: DNA damage-induced Lok/CHK2 activation compromises germline stem cell self-renewal and lineage differentiation.

FHA domain in Mnk plays dual functions in mediating embryonic DNA damage responses by means of its phosphopeptide-binding ability: activating Mnk in nucleus upon DNA damage &recruiting Mnk to multiple subcellular structures independently of DNA damage
Title: The FHA domain determines Drosophila Chk2/Mnk localization to key mitotic structures and is essential for early embryonic DNA damage responses.

nuclear retention involves Chk2-mediated inactivation of the Drosophila stem loop binding protein (SLBP), the levels of which are specifically depleted in damaged nuclei following Chk2 phosphorylation, an event that contributes to nuclear fallout.
Title: Developmentally regulated elimination of damaged nuclei involves a Chk2-dependent mechanism of mRNA nuclear retention.

We conclude that the primary responsibility for maintaining genome integrity in the male germline lies with Chk2, and that P53 is required to reconstitute the germline when cells are eliminated owing to unrepaired DNA damage.
Title: Chk2 and p53 regulate the transmission of healed chromosomes in the Drosophila male germline.

MOF controls Checkpoint protein2 and regulates genomic stability during early embryogenesis.
Title: Drosophila MOF controls Checkpoint protein2 and regulates genomic stability during early embryogenesis.

The following lok gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lok cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15023	NM_001259171.2 Latest version!	Drosophila melanogaster loki (lok), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa16926	NM_057871.4 Latest version!	Drosophila melanogaster loki (lok), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa16927	NM_057870.4 Latest version!	Drosophila melanogaster loki (lok), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa16927	NM_165318.3 Latest version!	Drosophila melanogaster loki (lok), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lok ( NM_001259171.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246100.1 Drosophila melanogaster loki (lok), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa15023
Clone ID Related Accession (Same CDS sequence)	NM_001259171.2
Accession Version	NM_001259171.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	loki, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001259171.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACGCG ACACACAAGG AACGCAGGGA ACCCAGAGTC AGGCCTCCAA TATCTGGACG 
CAGGTGGAGA GCCAGCCGAT GGAGAAGATA GTCTGGGGTC GATTGTACGG CAAGAACATT 
AAGATCAAAT CGCTGGATCT CAACAACGAC GAGTTCACCG CAGGACGAGG GGAGGCCAAC 
GACTTGATCC TCACGCTCAA CGATTTGCCC GAGAAAATCC TGACGCGCAT TTCCAAAGTG 
CATTTTATCA TAAAGCGGGC AAACTGCGAA CTTACCAATC CAGTTTATAT TCAGGATCTC 
TCACGCAACG GGACGTTTGT GAATAACGAG AAAATTGGCA CAAACAGGAT GCGAATCCTA 
AAGAATGACG ACGTAATTTC CCTGTCCCAC CCAACGTACA AAGCCTTTGT ATTCAAGGAT 
CTCAGCCCGA ATGAGTCGAT CGGGCTTCCC GAGGAGATTA ACAAGACGTA TTATGTAAAT 
CGCAAGCTGG GATCTGGAGC GTACGGACTG GTGCGTTTGG TCTATGACAC CCGCACCTGC 
CAGCAGTTTG CGATGAAAAT CGTTAAGAAG AATATGCTCT CTGGGGCGCG ACCCAGTACC 
AATTTCAGTG ATCCCGACCG GGTGCTGAAC GAAGCCAAGA TTATGAAGAA TCTTTCACAT 
CCGTGCGTGG TTCGCATGCA CGACATCGTG GACAAGCCGG ATTCTGTGTA TATGGTGCTG 
GAGTTTATGC GCGGCGGCGA CCTGCTTAAC CGGATCATCA GCAACAAACT ACTTAGCGAG 
GACATCTCGA AGCTTTACTT TTACCAAATG TGCCATGCCG TCAAGTACCT ACACGATCGG 
GGTAAAACAA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246100.1
CDS	381..1235
Translation	MARDTQGTQGTQSQASNIWTQVESQPMEKIVWGRLYGKNIKIKSLDLNNDEFTAGRGEANDLILTLNDLPEKILTRISKVHFIIKRANCELTNPVYIQDLSRNGTFVNNEKIGTNRMRILKNDDVISLSHPTYKAFVFKDLSPNESIGLPEEINKTYYVNRKLGSGAYGLVRLVYDTRTCQQFAMKIVKKNMLSGARPSTNFSDPDRVLNEAKIMKNLSHPCVVRMHDIVDKPDSVYMVLEFMRGGDLLNRIISNKLLSEDISKLYFYQMCHAVKYLHDRGKTN

Target ORF information:

RefSeq Version	NM_001259171.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster loki (lok), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259171.2

ORF Insert Sequence:

ATGGCACGCG ACACACAAGG AACGCAGGGA ACCCAGAGTC AGGCCTCCAA TATCTGGACG 
CAGGTGGAGA GCCAGCCGAT GGAGAAGATA GTCTGGGGTC GATTGTACGG CAAGAACATT 
AAGATCAAAT CGCTGGATCT CAACAACGAC GAGTTCACCG CAGGACGAGG GGAGGCCAAC 
GACTTGATCC TCACGCTCAA CGATTTGCCC GAGAAAATCC TGACGCGCAT TTCCAAAGTG 
CATTTTATCA TAAAGCGGGC AAACTGCGAA CTTACCAATC CAGTTTATAT TCAGGATCTC 
TCACGCAACG GGACGTTTGT GAATAACGAG AAAATTGGCA CAAACAGGAT GCGAATCCTA 
AAGAATGACG ACGTAATTTC CCTGTCCCAC CCAACGTACA AAGCCTTTGT ATTCAAGGAT 
CTCAGCCCGA ATGAGTCGAT CGGGCTTCCC GAGGAGATTA ACAAGACGTA TTATGTAAAT 
CGCAAGCTGG GATCTGGAGC GTACGGACTG GTGCGTTTGG TCTATGACAC CCGCACCTGC 
CAGCAGTTTG CGATGAAAAT CGTTAAGAAG AATATGCTCT CTGGGGCGCG ACCCAGTACC 
AATTTCAGTG ATCCCGACCG GGTGCTGAAC GAAGCCAAGA TTATGAAGAA TCTTTCACAT 
CCGTGCGTGG TTCGCATGCA CGACATCGTG GACAAGCCGG ATTCTGTGTA TATGGTGCTG 
GAGTTTATGC GCGGCGGCGA CCTGCTTAAC CGGATCATCA GCAACAAACT ACTTAGCGAG 
GACATCTCGA AGCTTTACTT TTACCAAATG TGCCATGCCG TCAAGTACCT ACACGATCGG 
GGTAAAACAA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lok ( NM_057871.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477219.1 Drosophila melanogaster loki (lok), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16926
Clone ID Related Accession (Same CDS sequence)	NM_057871.4
Accession Version	NM_057871.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	loki, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057871.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACGCG ACACACAAGG AACGCAGGGA ACCCAGAGTC AGGCCTCCAA TATCTGGACG 
CAGGTGGAGA GCCAGCCGAT GGAGAAGATA GTCTGGGGTC GATTGTACGG CAAGAACATT 
AAGATCAAAT CGCTGGGTAC GAGTTCAAAA TACCGAATCA TTTATACGCA TTCGTCCTTT 
TCTGTTGATC TCAACAACGA CGAGTTCACC GCAGGACGAG GGGAGGCCAA CGACTTGATC 
CTCACGCTCA ACGATTTGCC CGAGAAAATC CTGACGCGCA TTTCCAAAGT GCATTTTATC 
ATAAAGCGGG CAAACTGCGA ACTTACCAAT CCAGTTTATA TTCAGGATCT CTCACGCAAC 
GGGACGTTTG TGAATAACGA GAAAATTGGC ACAAACAGGA TGCGAATCCT AAAGAATGAC 
GACGTAATTT CCCTGTCCCA CCCAACGTAC AAAGCCTTTG TATTCAAGGA TCTCAGCCCG 
AATGAGTCGA TCGGGCTTCC CGAGGAGATT AACAAGACGT ATTATGTAAA TCGCAAGCTG 
GGATCTGGAG CGTACGGACT GGTGCGTTTG GTCTATGACA CCCGCACCTG CCAGCAGTTT 
GCGATGAAAA TCGTTAAGAA GAATATGCTC TCTGGGGCGC GACCCAGTAC CAATTTCAGT 
GATCCCGACC GGGTGCTGAA CGAAGCCAAG ATTATGAAGA ATCTTTCACA TCCGTGCGTG 
GTTCGCATGC ACGACATCGT GGACAAGCCG GATTCTGTGT ATATGGTGCT GGAGTTTATG 
CGCGGCGGCG ACCTGCTTAA CCGGATCATC AGCAACAAAC TACTTAGCGA GGACATCTCG 
AAGCTTTACT TTTACCAAAT GTGCCATGCC GTCAAGTACC TACACGATCG GGGCATCACC 
CATCGTGACC TTAAGCCTGA CAATGTTTTG CTGGAGACCA ATGACGAGGA GACCCTACTG 
AAGGTCTCCG ATTTCGGGCT GAGTAAGTTT GTGCAGAAGG ACTCGGTTAT GCGCACGCTC 
TGTGGCACAC CTCTCTACGT GGCCCCTGAG GTGCTGATTA CCGGCGGCCG AGAAGCCTAC 
ACCAAGAAAG TGGACATTTG GAGCTTGGGA GTGGTGCTAT TTACTTGCCT GAGTGGCACT 
CTGCCCTTTT CGGACGAGTA CGGCACCCCG GCGGCCCAGC AAATAAAGAA GGGCAGATTC 
GCGTATGGAC ACCCTTCTTG GAAGAGTGTC TCGCAGCGCG CCAAGCTGCT GATCAACCAA 
ATGCTCATTG TGGATCCCGA GAGGCGACCC TCAATCGACG ACGTTTTGCA GAGCAGCTGG 
CTGCGCGATG CGCCCATGCT GCAGAAGGCG AAGAGGCTGA TGAAGCTCGA TGGCATGGAA 
ATCGAGGAGG AGAACTTCCT TGAGCCACCC ACCAAGCGAT CGCGACGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477219.1
CDS	395..1825
Translation	MARDTQGTQGTQSQASNIWTQVESQPMEKIVWGRLYGKNIKIKSLGTSSKYRIIYTHSSFSVDLNNDEFTAGRGEANDLILTLNDLPEKILTRISKVHFIIKRANCELTNPVYIQDLSRNGTFVNNEKIGTNRMRILKNDDVISLSHPTYKAFVFKDLSPNESIGLPEEINKTYYVNRKLGSGAYGLVRLVYDTRTCQQFAMKIVKKNMLSGARPSTNFSDPDRVLNEAKIMKNLSHPCVVRMHDIVDKPDSVYMVLEFMRGGDLLNRIISNKLLSEDISKLYFYQMCHAVKYLHDRGITHRDLKPDNVLLETNDEETLLKVSDFGLSKFVQKDSVMRTLCGTPLYVAPEVLITGGREAYTKKVDIWSLGVVLFTCLSGTLPFSDEYGTPAAQQIKKGRFAYGHPSWKSVSQRAKLLINQMLIVDPERRPSIDDVLQSSWLRDAPMLQKAKRLMKLDGMEIEEENFLEPPTKRSRR

Target ORF information:

RefSeq Version	NM_057871.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster loki (lok), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057871.4

ORF Insert Sequence:

ATGGCACGCG ACACACAAGG AACGCAGGGA ACCCAGAGTC AGGCCTCCAA TATCTGGACG 
CAGGTGGAGA GCCAGCCGAT GGAGAAGATA GTCTGGGGTC GATTGTACGG CAAGAACATT 
AAGATCAAAT CGCTGGGTAC GAGTTCAAAA TACCGAATCA TTTATACGCA TTCGTCCTTT 
TCTGTTGATC TCAACAACGA CGAGTTCACC GCAGGACGAG GGGAGGCCAA CGACTTGATC 
CTCACGCTCA ACGATTTGCC CGAGAAAATC CTGACGCGCA TTTCCAAAGT GCATTTTATC 
ATAAAGCGGG CAAACTGCGA ACTTACCAAT CCAGTTTATA TTCAGGATCT CTCACGCAAC 
GGGACGTTTG TGAATAACGA GAAAATTGGC ACAAACAGGA TGCGAATCCT AAAGAATGAC 
GACGTAATTT CCCTGTCCCA CCCAACGTAC AAAGCCTTTG TATTCAAGGA TCTCAGCCCG 
AATGAGTCGA TCGGGCTTCC CGAGGAGATT AACAAGACGT ATTATGTAAA TCGCAAGCTG 
GGATCTGGAG CGTACGGACT GGTGCGTTTG GTCTATGACA CCCGCACCTG CCAGCAGTTT 
GCGATGAAAA TCGTTAAGAA GAATATGCTC TCTGGGGCGC GACCCAGTAC CAATTTCAGT 
GATCCCGACC GGGTGCTGAA CGAAGCCAAG ATTATGAAGA ATCTTTCACA TCCGTGCGTG 
GTTCGCATGC ACGACATCGT GGACAAGCCG GATTCTGTGT ATATGGTGCT GGAGTTTATG 
CGCGGCGGCG ACCTGCTTAA CCGGATCATC AGCAACAAAC TACTTAGCGA GGACATCTCG 
AAGCTTTACT TTTACCAAAT GTGCCATGCC GTCAAGTACC TACACGATCG GGGCATCACC 
CATCGTGACC TTAAGCCTGA CAATGTTTTG CTGGAGACCA ATGACGAGGA GACCCTACTG 
AAGGTCTCCG ATTTCGGGCT GAGTAAGTTT GTGCAGAAGG ACTCGGTTAT GCGCACGCTC 
TGTGGCACAC CTCTCTACGT GGCCCCTGAG GTGCTGATTA CCGGCGGCCG AGAAGCCTAC 
ACCAAGAAAG TGGACATTTG GAGCTTGGGA GTGGTGCTAT TTACTTGCCT GAGTGGCACT 
CTGCCCTTTT CGGACGAGTA CGGCACCCCG GCGGCCCAGC AAATAAAGAA GGGCAGATTC 
GCGTATGGAC ACCCTTCTTG GAAGAGTGTC TCGCAGCGCG CCAAGCTGCT GATCAACCAA 
ATGCTCATTG TGGATCCCGA GAGGCGACCC TCAATCGACG ACGTTTTGCA GAGCAGCTGG 
CTGCGCGATG CGCCCATGCT GCAGAAGGCG AAGAGGCTGA TGAAGCTCGA TGGCATGGAA 
ATCGAGGAGG AGAACTTCCT TGAGCCACCC ACCAAGCGAT CGCGACGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lok ( NM_057870.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477218.1 Drosophila melanogaster loki (lok), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16927
Clone ID Related Accession (Same CDS sequence)	NM_057870.4 , NM_165318.3
Accession Version	NM_057870.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	loki, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057870.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACGCG ACACACAAGG AACGCAGGGA ACCCAGAGTC AGGCCTCCAA TATCTGGACG 
CAGGTGGAGA GCCAGCCGAT GGAGAAGATA GTCTGGGGTC GATTGTACGG CAAGAACATT 
AAGATCAAAT CGCTGGATCT CAACAACGAC GAGTTCACCG CAGGACGAGG GGAGGCCAAC 
GACTTGATCC TCACGCTCAA CGATTTGCCC GAGAAAATCC TGACGCGCAT TTCCAAAGTG 
CATTTTATCA TAAAGCGGGC AAACTGCGAA CTTACCAATC CAGTTTATAT TCAGGATCTC 
TCACGCAACG GGACGTTTGT GAATAACGAG AAAATTGGCA CAAACAGGAT GCGAATCCTA 
AAGAATGACG ACGTAATTTC CCTGTCCCAC CCAACGTACA AAGCCTTTGT ATTCAAGGAT 
CTCAGCCCGA ATGAGTCGAT CGGGCTTCCC GAGGAGATTA ACAAGACGTA TTATGTAAAT 
CGCAAGCTGG GATCTGGAGC GTACGGACTG GTGCGTTTGG TCTATGACAC CCGCACCTGC 
CAGCAGTTTG CGATGAAAAT CGTTAAGAAG AATATGCTCT CTGGGGCGCG ACCCAGTACC 
AATTTCAGTG ATCCCGACCG GGTGCTGAAC GAAGCCAAGA TTATGAAGAA TCTTTCACAT 
CCGTGCGTGG TTCGCATGCA CGACATCGTG GACAAGCCGG ATTCTGTGTA TATGGTGCTG 
GAGTTTATGC GCGGCGGCGA CCTGCTTAAC CGGATCATCA GCAACAAACT ACTTAGCGAG 
GACATCTCGA AGCTTTACTT TTACCAAATG TGCCATGCCG TCAAGTACCT ACACGATCGG 
GGCATCACCC ATCGTGACCT TAAGCCTGAC AATGTTTTGC TGGAGACCAA TGACGAGGAG 
ACCCTACTGA AGGTCTCCGA TTTCGGGCTG AGTAAGTTTG TGCAGAAGGA CTCGGTTATG 
CGCACGCTCT GTGGCACACC TCTCTACGTG GCCCCTGAGG TGCTGATTAC CGGCGGCCGA 
GAAGCCTACA CCAAGAAAGT GGACATTTGG AGCTTGGGAG TGGTGCTATT TACTTGCCTG 
AGTGGCACTC TGCCCTTTTC GGACGAGTAC GGCACCCCGG CGGCCCAGCA AATAAAGAAG 
GGCAGATTCG CGTATGGACA CCCTTCTTGG AAGAGTGTCT CGCAGCGCGC CAAGCTGCTG 
ATCAACCAAA TGCTCATTGT GGATCCCGAG AGGCGACCCT CAATCGACGA CGTTTTGCAG 
AGCAGCTGGC TGCGCGATGC GCCCATGCTG CAGAAGGCGA AGAGGCTGAT GAAGCTCGAT 
GGCATGGAAA TCGAGGAGGA GAACTTCCTT GAGCCACCCA CCAAGCGATC GCGACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477218.1
CDS	320..1699
Translation	MARDTQGTQGTQSQASNIWTQVESQPMEKIVWGRLYGKNIKIKSLDLNNDEFTAGRGEANDLILTLNDLPEKILTRISKVHFIIKRANCELTNPVYIQDLSRNGTFVNNEKIGTNRMRILKNDDVISLSHPTYKAFVFKDLSPNESIGLPEEINKTYYVNRKLGSGAYGLVRLVYDTRTCQQFAMKIVKKNMLSGARPSTNFSDPDRVLNEAKIMKNLSHPCVVRMHDIVDKPDSVYMVLEFMRGGDLLNRIISNKLLSEDISKLYFYQMCHAVKYLHDRGITHRDLKPDNVLLETNDEETLLKVSDFGLSKFVQKDSVMRTLCGTPLYVAPEVLITGGREAYTKKVDIWSLGVVLFTCLSGTLPFSDEYGTPAAQQIKKGRFAYGHPSWKSVSQRAKLLINQMLIVDPERRPSIDDVLQSSWLRDAPMLQKAKRLMKLDGMEIEEENFLEPPTKRSRR

Target ORF information:

RefSeq Version	NM_057870.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster loki (lok), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057870.4

ORF Insert Sequence:

ATGGCACGCG ACACACAAGG AACGCAGGGA ACCCAGAGTC AGGCCTCCAA TATCTGGACG 
CAGGTGGAGA GCCAGCCGAT GGAGAAGATA GTCTGGGGTC GATTGTACGG CAAGAACATT 
AAGATCAAAT CGCTGGATCT CAACAACGAC GAGTTCACCG CAGGACGAGG GGAGGCCAAC 
GACTTGATCC TCACGCTCAA CGATTTGCCC GAGAAAATCC TGACGCGCAT TTCCAAAGTG 
CATTTTATCA TAAAGCGGGC AAACTGCGAA CTTACCAATC CAGTTTATAT TCAGGATCTC 
TCACGCAACG GGACGTTTGT GAATAACGAG AAAATTGGCA CAAACAGGAT GCGAATCCTA 
AAGAATGACG ACGTAATTTC CCTGTCCCAC CCAACGTACA AAGCCTTTGT ATTCAAGGAT 
CTCAGCCCGA ATGAGTCGAT CGGGCTTCCC GAGGAGATTA ACAAGACGTA TTATGTAAAT 
CGCAAGCTGG GATCTGGAGC GTACGGACTG GTGCGTTTGG TCTATGACAC CCGCACCTGC 
CAGCAGTTTG CGATGAAAAT CGTTAAGAAG AATATGCTCT CTGGGGCGCG ACCCAGTACC 
AATTTCAGTG ATCCCGACCG GGTGCTGAAC GAAGCCAAGA TTATGAAGAA TCTTTCACAT 
CCGTGCGTGG TTCGCATGCA CGACATCGTG GACAAGCCGG ATTCTGTGTA TATGGTGCTG 
GAGTTTATGC GCGGCGGCGA CCTGCTTAAC CGGATCATCA GCAACAAACT ACTTAGCGAG 
GACATCTCGA AGCTTTACTT TTACCAAATG TGCCATGCCG TCAAGTACCT ACACGATCGG 
GGCATCACCC ATCGTGACCT TAAGCCTGAC AATGTTTTGC TGGAGACCAA TGACGAGGAG 
ACCCTACTGA AGGTCTCCGA TTTCGGGCTG AGTAAGTTTG TGCAGAAGGA CTCGGTTATG 
CGCACGCTCT GTGGCACACC TCTCTACGTG GCCCCTGAGG TGCTGATTAC CGGCGGCCGA 
GAAGCCTACA CCAAGAAAGT GGACATTTGG AGCTTGGGAG TGGTGCTATT TACTTGCCTG 
AGTGGCACTC TGCCCTTTTC GGACGAGTAC GGCACCCCGG CGGCCCAGCA AATAAAGAAG 
GGCAGATTCG CGTATGGACA CCCTTCTTGG AAGAGTGTCT CGCAGCGCGC CAAGCTGCTG 
ATCAACCAAA TGCTCATTGT GGATCCCGAG AGGCGACCCT CAATCGACGA CGTTTTGCAG 
AGCAGCTGGC TGCGCGATGC GCCCATGCTG CAGAAGGCGA AGAGGCTGAT GAAGCTCGAT 
GGCATGGAAA TCGAGGAGGA GAACTTCCTT GAGCCACCCA CCAAGCGATC GCGACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lok ( NM_165318.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724241.1 Drosophila melanogaster loki (lok), transcript variant C, mRNA.

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CloneID	OFa16927
Clone ID Related Accession (Same CDS sequence)	NM_057870.4 , NM_165318.3
Accession Version	NM_165318.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	loki, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165318.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACGCG ACACACAAGG AACGCAGGGA ACCCAGAGTC AGGCCTCCAA TATCTGGACG 
CAGGTGGAGA GCCAGCCGAT GGAGAAGATA GTCTGGGGTC GATTGTACGG CAAGAACATT 
AAGATCAAAT CGCTGGATCT CAACAACGAC GAGTTCACCG CAGGACGAGG GGAGGCCAAC 
GACTTGATCC TCACGCTCAA CGATTTGCCC GAGAAAATCC TGACGCGCAT TTCCAAAGTG 
CATTTTATCA TAAAGCGGGC AAACTGCGAA CTTACCAATC CAGTTTATAT TCAGGATCTC 
TCACGCAACG GGACGTTTGT GAATAACGAG AAAATTGGCA CAAACAGGAT GCGAATCCTA 
AAGAATGACG ACGTAATTTC CCTGTCCCAC CCAACGTACA AAGCCTTTGT ATTCAAGGAT 
CTCAGCCCGA ATGAGTCGAT CGGGCTTCCC GAGGAGATTA ACAAGACGTA TTATGTAAAT 
CGCAAGCTGG GATCTGGAGC GTACGGACTG GTGCGTTTGG TCTATGACAC CCGCACCTGC 
CAGCAGTTTG CGATGAAAAT CGTTAAGAAG AATATGCTCT CTGGGGCGCG ACCCAGTACC 
AATTTCAGTG ATCCCGACCG GGTGCTGAAC GAAGCCAAGA TTATGAAGAA TCTTTCACAT 
CCGTGCGTGG TTCGCATGCA CGACATCGTG GACAAGCCGG ATTCTGTGTA TATGGTGCTG 
GAGTTTATGC GCGGCGGCGA CCTGCTTAAC CGGATCATCA GCAACAAACT ACTTAGCGAG 
GACATCTCGA AGCTTTACTT TTACCAAATG TGCCATGCCG TCAAGTACCT ACACGATCGG 
GGCATCACCC ATCGTGACCT TAAGCCTGAC AATGTTTTGC TGGAGACCAA TGACGAGGAG 
ACCCTACTGA AGGTCTCCGA TTTCGGGCTG AGTAAGTTTG TGCAGAAGGA CTCGGTTATG 
CGCACGCTCT GTGGCACACC TCTCTACGTG GCCCCTGAGG TGCTGATTAC CGGCGGCCGA 
GAAGCCTACA CCAAGAAAGT GGACATTTGG AGCTTGGGAG TGGTGCTATT TACTTGCCTG 
AGTGGCACTC TGCCCTTTTC GGACGAGTAC GGCACCCCGG CGGCCCAGCA AATAAAGAAG 
GGCAGATTCG CGTATGGACA CCCTTCTTGG AAGAGTGTCT CGCAGCGCGC CAAGCTGCTG 
ATCAACCAAA TGCTCATTGT GGATCCCGAG AGGCGACCCT CAATCGACGA CGTTTTGCAG 
AGCAGCTGGC TGCGCGATGC GCCCATGCTG CAGAAGGCGA AGAGGCTGAT GAAGCTCGAT 
GGCATGGAAA TCGAGGAGGA GAACTTCCTT GAGCCACCCA CCAAGCGATC GCGACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724241.1
CDS	381..1760
Translation	MARDTQGTQGTQSQASNIWTQVESQPMEKIVWGRLYGKNIKIKSLDLNNDEFTAGRGEANDLILTLNDLPEKILTRISKVHFIIKRANCELTNPVYIQDLSRNGTFVNNEKIGTNRMRILKNDDVISLSHPTYKAFVFKDLSPNESIGLPEEINKTYYVNRKLGSGAYGLVRLVYDTRTCQQFAMKIVKKNMLSGARPSTNFSDPDRVLNEAKIMKNLSHPCVVRMHDIVDKPDSVYMVLEFMRGGDLLNRIISNKLLSEDISKLYFYQMCHAVKYLHDRGITHRDLKPDNVLLETNDEETLLKVSDFGLSKFVQKDSVMRTLCGTPLYVAPEVLITGGREAYTKKVDIWSLGVVLFTCLSGTLPFSDEYGTPAAQQIKKGRFAYGHPSWKSVSQRAKLLINQMLIVDPERRPSIDDVLQSSWLRDAPMLQKAKRLMKLDGMEIEEENFLEPPTKRSRR

Target ORF information:

RefSeq Version	NM_165318.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster loki (lok), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165318.3

ORF Insert Sequence:

ATGGCACGCG ACACACAAGG AACGCAGGGA ACCCAGAGTC AGGCCTCCAA TATCTGGACG 
CAGGTGGAGA GCCAGCCGAT GGAGAAGATA GTCTGGGGTC GATTGTACGG CAAGAACATT 
AAGATCAAAT CGCTGGATCT CAACAACGAC GAGTTCACCG CAGGACGAGG GGAGGCCAAC 
GACTTGATCC TCACGCTCAA CGATTTGCCC GAGAAAATCC TGACGCGCAT TTCCAAAGTG 
CATTTTATCA TAAAGCGGGC AAACTGCGAA CTTACCAATC CAGTTTATAT TCAGGATCTC 
TCACGCAACG GGACGTTTGT GAATAACGAG AAAATTGGCA CAAACAGGAT GCGAATCCTA 
AAGAATGACG ACGTAATTTC CCTGTCCCAC CCAACGTACA AAGCCTTTGT ATTCAAGGAT 
CTCAGCCCGA ATGAGTCGAT CGGGCTTCCC GAGGAGATTA ACAAGACGTA TTATGTAAAT 
CGCAAGCTGG GATCTGGAGC GTACGGACTG GTGCGTTTGG TCTATGACAC CCGCACCTGC 
CAGCAGTTTG CGATGAAAAT CGTTAAGAAG AATATGCTCT CTGGGGCGCG ACCCAGTACC 
AATTTCAGTG ATCCCGACCG GGTGCTGAAC GAAGCCAAGA TTATGAAGAA TCTTTCACAT 
CCGTGCGTGG TTCGCATGCA CGACATCGTG GACAAGCCGG ATTCTGTGTA TATGGTGCTG 
GAGTTTATGC GCGGCGGCGA CCTGCTTAAC CGGATCATCA GCAACAAACT ACTTAGCGAG 
GACATCTCGA AGCTTTACTT TTACCAAATG TGCCATGCCG TCAAGTACCT ACACGATCGG 
GGCATCACCC ATCGTGACCT TAAGCCTGAC AATGTTTTGC TGGAGACCAA TGACGAGGAG 
ACCCTACTGA AGGTCTCCGA TTTCGGGCTG AGTAAGTTTG TGCAGAAGGA CTCGGTTATG 
CGCACGCTCT GTGGCACACC TCTCTACGTG GCCCCTGAGG TGCTGATTAC CGGCGGCCGA 
GAAGCCTACA CCAAGAAAGT GGACATTTGG AGCTTGGGAG TGGTGCTATT TACTTGCCTG 
AGTGGCACTC TGCCCTTTTC GGACGAGTAC GGCACCCCGG CGGCCCAGCA AATAAAGAAG 
GGCAGATTCG CGTATGGACA CCCTTCTTGG AAGAGTGTCT CGCAGCGCGC CAAGCTGCTG 
ATCAACCAAA TGCTCATTGT GGATCCCGAG AGGCGACCCT CAATCGACGA CGTTTTGCAG 
AGCAGCTGGC TGCGCGATGC GCCCATGCTG CAGAAGGCGA AGAGGCTGAT GAAGCTCGAT 
GGCATGGAAA TCGAGGAGGA GAACTTCCTT GAGCCACCCA CCAAGCGATC GCGACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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lok cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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