barr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	barr
Entrez Gene ID	35287
Full Name	barren
Synonyms	38B.3,Barr,Barren,CAP-H,CG10726,CG14684,Cap-H,CapH,Dmel\CG10726,dCap-H
General protein information	Preferred Names barren Names CG10726 gene product from transcript CG10726-RC CG10726-PA CG10726-PB CG10726-PC barr-PA barr-PB barr-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38B1-38B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057758.5	NP_477106.1	barren, isoform A
NM_165317.3	NP_724240.1	barren, isoform B
NM_001299165.1	NP_001286094.1	barren, isoform C

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ncaph	NP_659067.2
Gallus gallus (chicken)	NCAPH	NP_001244261.1
Drosophila melanogaster (fruit fly)	barr	NP_477106.1
Canis lupus familiaris (dog)	NCAPH	XP_532948.4
Bos taurus (cattle)	NCAPH	NP_001029385.1
Pan troglodytes (chimpanzee)	NCAPH	XP_001148661.1
Danio rerio (zebrafish)	ncaph	NP_001073665.1
Xenopus tropicalis (tropical clawed frog)	ncaph	NP_001007981.2
Homo sapiens (human)	NCAPH	NP_056156.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011508	XP_557414.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

analysis of Barren/CAP-H-depleted cells showed that mitotic chromosomes are able to condense but fail to resolve sister chromatids
Title: The condensin I subunit Barren/CAP-H is essential for the structural integrity of centromeric heterochromatin during mitosis.

The following barr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the barr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16925	NM_057758.5 Latest version!	Drosophila melanogaster barren (barr), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16925	NM_165317.3 Latest version!	Drosophila melanogaster barren (barr), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16925	NM_001299165.1 Latest version!	Drosophila melanogaster barren (barr), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

barr ( NM_057758.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477106.1 Drosophila melanogaster barren (barr), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16925
Clone ID Related Accession (Same CDS sequence)	NM_057758.5 , NM_165317.3 , NM_001299165.1
Accession Version	NM_057758.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	barren, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057758.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTCTGC CCCGCTTAGA AACTCCGCTC AGGCGGTCCG CAGTAGGCAG TTACCAGGAG 
GGGGTGTCCC GCATGCTAAC CCCCTTTAAC GACGACGAGG CGGAAAGGCG GGAAGCCCGA 
CGTCGCACGC TCCTTCAGCA GCATCACCGG TCGAGCACTC TGGAGTCCAT CGAGGACAAT 
GAGACCATCA AAAATTGCTT GGAGCTCTAC AATGGCAACA AGGTGAGCAA GGACAACGCC 
TGGAACCTGA TGCTCATCGA TTCGTTGGCC AATTTGCTGG ATCACCATCA CAAGCGCATG 
AGCAACTTCA AGATGGCTGG TTCCTCTTTA GAAGCCTCTT CCAAGGTTTA CGGCCTGCGT 
GTGGACTCCA TCTACTTGGA CGCCATGCGC ATTTCAGCTG GCTTGAGTGC ACGAACTCTT 
ACGGATAAGC AAATAAATGC TGCCGAGGAT GATGATGGCC CCCAAGGTGA ACAGGCTACT 
GGCGAGGGTC AGGACTCAGC GCAACAGGCA GCAAAAGAAG CAGCACCGAA GCCCAAACGG 
CAAAAGAAAC CCATATCCAC CGTGACCAAA AACAGAGAAA CGCTGAACAG CCGGCTGGAT 
ACGGCTCCCT TGCAGGATCC TGTGTTTGGC AAGCTGAACT CCACTGTGGG CTCTATAAAT 
GCTTCCAATC GTCTGATGCA CAACATTCTT CCTAGTTTCG ACTCTGAATT GCGCTTGCGT 
ACCACCTACA ATTTTTGGAA CAGCGAAGAG TCCACCGAGG AGGTCCAGGA CCACACTACA 
CTCAATGCAG AAATGGAACA ATGGCCAGCC ACATCACTTA TGAGCACCAA TCTTATGCGC 
AAACTGCTGC CACATGCTGA AAGGTCAAAT CTGCGACCGC TGCATACGGG CTACATCATC 
ACAAGTGCTC CCAATCCCAA GTCGGCAAAT GAAAAGGCCG CCGAGGTAGT GCAGGATGAA 
GATCATGACG AGGGTCTGGA TAATGCCGAC GATATGTGTG TAAATGAGAT TTCCATGGCC 
TTTGACATCA ATGCCGAGTG TGAGCCCATG CCGGACCTGG ATGGACCACC GCCTCTGGTG 
CTGGAGGTGG ACTCCAATGA GCTGGAAGAG CTCACTGCCG AGGAGCAAAT GGTGATCAAT 
AATTGCCGTC GGCTACGTAA GCAGACAGAG TTCATCGAGG ATTTGCGACC GGTAGACGGG 
AATTCCAAGC TAGAGTACTC CTACAGGCCG ATGGACCAGA TCTCCCAGTT TTGGGCCGGC 
CCCTCGCATT GGAAGTTTAA GCGGACTCGC CCGCGCAGCA CTTTCTCGCA GACCAATGGA 
CAGGTGGACA CTCAGCCTAT TCGCACTCAA CGAGCGAAGA AGTCAGCGCA TCTGAATGCC 
AACCGAAGAG CCAAGGCTTT GGACTATGGC AATGTGACGG AAAACTTCTT CCAACAGCTG 
GACACCACCA TAAGGCAGCG CAAGGCGAAT TTCCAAAAGA AGTGGGATCC CCGAAAGCTG 
ATATTGCCCA CCAAGTTCGA ATTGGACCCG GATCTTTTCT TCAAGTACGA ATCCGCTCCC 
AGCATAAAGT TGTCGAAACG CGCAGGTGAA CCTGATTCGG ATGAGGGTGG AGATCTTGGC 
ATCGATATGG ATGCGGATAT GCACCACGAT GATGACAATG ACCAGGAGCT ATTCAACAAC 
GAGCATTTTA CCGACGCCGT TCCCGCCAAC GTGAGCGTTA TTGCCGCGAT TGCTGCGGAG 
CAAGCGGCCG AGGCAAGCAT GATGAACGTG TCCGCGGGTG AGATTGGCCT CACCCAGATG 
AACGCCACTT GCAACAACAC AGTCTTCGAG ATCGGCACCG AGTTCGAGGG TGCGCCGTCG 
CAGGTTGCCA AAGTGATTGT TCCATTCGCG AAGAGAGCCA AGGTTATAGA CATGAAGAAT 
CTGAAAAAGT CCTGCAACTC TCTAATACAG AAACAATTGC TAAACGCTGT GCCGGAGGAG 
ACGATACCCT CGCATCCCAA GAAAAAGGGC GAACACTATT CAAAGGGTTT CGCGAGTTTT 
CAGCAGGTGT ATCAGAAGCT GCCCGACCTT TTGACCACCA AAATGTCAGA CTCGCTGTCC 
CCGTCGGTGG CATTCTATGC GGTTCTGCAT TTGGCCAACG ATTTGAAACT GCGGCTAATT 
CCGCAGGAGG ACTTGGAGGA CTTTCAAATT CGCCAGGTGT TGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477106.1
CDS	151..2358
Translation	MTLPRLETPLRRSAVGSYQEGVSRMLTPFNDDEAERREARRRTLLQQHHRSSTLESIEDNETIKNCLELYNGNKVSKDNAWNLMLIDSLANLLDHHHKRMSNFKMAGSSLEASSKVYGLRVDSIYLDAMRISAGLSARTLTDKQINAAEDDDGPQGEQATGEGQDSAQQAAKEAAPKPKRQKKPISTVTKNRETLNSRLDTAPLQDPVFGKLNSTVGSINASNRLMHNILPSFDSELRLRTTYNFWNSEESTEEVQDHTTLNAEMEQWPATSLMSTNLMRKLLPHAERSNLRPLHTGYIITSAPNPKSANEKAAEVVQDEDHDEGLDNADDMCVNEISMAFDINAECEPMPDLDGPPPLVLEVDSNELEELTAEEQMVINNCRRLRKQTEFIEDLRPVDGNSKLEYSYRPMDQISQFWAGPSHWKFKRTRPRSTFSQTNGQVDTQPIRTQRAKKSAHLNANRRAKALDYGNVTENFFQQLDTTIRQRKANFQKKWDPRKLILPTKFELDPDLFFKYESAPSIKLSKRAGEPDSDEGGDLGIDMDADMHHDDDNDQELFNNEHFTDAVPANVSVIAAIAAEQAAEASMMNVSAGEIGLTQMNATCNNTVFEIGTEFEGAPSQVAKVIVPFAKRAKVIDMKNLKKSCNSLIQKQLLNAVPEETIPSHPKKKGEHYSKGFASFQQVYQKLPDLLTTKMSDSLSPSVAFYAVLHLANDLKLRLIPQEDLEDFQIRQVLD

Target ORF information:

RefSeq Version	NM_057758.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster barren (barr), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057758.5

ORF Insert Sequence:

ATGACTCTGC CCCGCTTAGA AACTCCGCTC AGGCGGTCCG CAGTAGGCAG TTACCAGGAG 
GGGGTGTCCC GCATGCTAAC CCCCTTTAAC GACGACGAGG CGGAAAGGCG GGAAGCCCGA 
CGTCGCACGC TCCTTCAGCA GCATCACCGG TCGAGCACTC TGGAGTCCAT CGAGGACAAT 
GAGACCATCA AAAATTGCTT GGAGCTCTAC AATGGCAACA AGGTGAGCAA GGACAACGCC 
TGGAACCTGA TGCTCATCGA TTCGTTGGCC AATTTGCTGG ATCACCATCA CAAGCGCATG 
AGCAACTTCA AGATGGCTGG TTCCTCTTTA GAAGCCTCTT CCAAGGTTTA CGGCCTGCGT 
GTGGACTCCA TCTACTTGGA CGCCATGCGC ATTTCAGCTG GCTTGAGTGC ACGAACTCTT 
ACGGATAAGC AAATAAATGC TGCCGAGGAT GATGATGGCC CCCAAGGTGA ACAGGCTACT 
GGCGAGGGTC AGGACTCAGC GCAACAGGCA GCAAAAGAAG CAGCACCGAA GCCCAAACGG 
CAAAAGAAAC CCATATCCAC CGTGACCAAA AACAGAGAAA CGCTGAACAG CCGGCTGGAT 
ACGGCTCCCT TGCAGGATCC TGTGTTTGGC AAGCTGAACT CCACTGTGGG CTCTATAAAT 
GCTTCCAATC GTCTGATGCA CAACATTCTT CCTAGTTTCG ACTCTGAATT GCGCTTGCGT 
ACCACCTACA ATTTTTGGAA CAGCGAAGAG TCCACCGAGG AGGTCCAGGA CCACACTACA 
CTCAATGCAG AAATGGAACA ATGGCCAGCC ACATCACTTA TGAGCACCAA TCTTATGCGC 
AAACTGCTGC CACATGCTGA AAGGTCAAAT CTGCGACCGC TGCATACGGG CTACATCATC 
ACAAGTGCTC CCAATCCCAA GTCGGCAAAT GAAAAGGCCG CCGAGGTAGT GCAGGATGAA 
GATCATGACG AGGGTCTGGA TAATGCCGAC GATATGTGTG TAAATGAGAT TTCCATGGCC 
TTTGACATCA ATGCCGAGTG TGAGCCCATG CCGGACCTGG ATGGACCACC GCCTCTGGTG 
CTGGAGGTGG ACTCCAATGA GCTGGAAGAG CTCACTGCCG AGGAGCAAAT GGTGATCAAT 
AATTGCCGTC GGCTACGTAA GCAGACAGAG TTCATCGAGG ATTTGCGACC GGTAGACGGG 
AATTCCAAGC TAGAGTACTC CTACAGGCCG ATGGACCAGA TCTCCCAGTT TTGGGCCGGC 
CCCTCGCATT GGAAGTTTAA GCGGACTCGC CCGCGCAGCA CTTTCTCGCA GACCAATGGA 
CAGGTGGACA CTCAGCCTAT TCGCACTCAA CGAGCGAAGA AGTCAGCGCA TCTGAATGCC 
AACCGAAGAG CCAAGGCTTT GGACTATGGC AATGTGACGG AAAACTTCTT CCAACAGCTG 
GACACCACCA TAAGGCAGCG CAAGGCGAAT TTCCAAAAGA AGTGGGATCC CCGAAAGCTG 
ATATTGCCCA CCAAGTTCGA ATTGGACCCG GATCTTTTCT TCAAGTACGA ATCCGCTCCC 
AGCATAAAGT TGTCGAAACG CGCAGGTGAA CCTGATTCGG ATGAGGGTGG AGATCTTGGC 
ATCGATATGG ATGCGGATAT GCACCACGAT GATGACAATG ACCAGGAGCT ATTCAACAAC 
GAGCATTTTA CCGACGCCGT TCCCGCCAAC GTGAGCGTTA TTGCCGCGAT TGCTGCGGAG 
CAAGCGGCCG AGGCAAGCAT GATGAACGTG TCCGCGGGTG AGATTGGCCT CACCCAGATG 
AACGCCACTT GCAACAACAC AGTCTTCGAG ATCGGCACCG AGTTCGAGGG TGCGCCGTCG 
CAGGTTGCCA AAGTGATTGT TCCATTCGCG AAGAGAGCCA AGGTTATAGA CATGAAGAAT 
CTGAAAAAGT CCTGCAACTC TCTAATACAG AAACAATTGC TAAACGCTGT GCCGGAGGAG 
ACGATACCCT CGCATCCCAA GAAAAAGGGC GAACACTATT CAAAGGGTTT CGCGAGTTTT 
CAGCAGGTGT ATCAGAAGCT GCCCGACCTT TTGACCACCA AAATGTCAGA CTCGCTGTCC 
CCGTCGGTGG CATTCTATGC GGTTCTGCAT TTGGCCAACG ATTTGAAACT GCGGCTAATT 
CCGCAGGAGG ACTTGGAGGA CTTTCAAATT CGCCAGGTGT TGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

barr ( NM_165317.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724240.1 Drosophila melanogaster barren (barr), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16925
Clone ID Related Accession (Same CDS sequence)	NM_057758.5 , NM_165317.3 , NM_001299165.1
Accession Version	NM_165317.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	barren, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165317.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTCTGC CCCGCTTAGA AACTCCGCTC AGGCGGTCCG CAGTAGGCAG TTACCAGGAG 
GGGGTGTCCC GCATGCTAAC CCCCTTTAAC GACGACGAGG CGGAAAGGCG GGAAGCCCGA 
CGTCGCACGC TCCTTCAGCA GCATCACCGG TCGAGCACTC TGGAGTCCAT CGAGGACAAT 
GAGACCATCA AAAATTGCTT GGAGCTCTAC AATGGCAACA AGGTGAGCAA GGACAACGCC 
TGGAACCTGA TGCTCATCGA TTCGTTGGCC AATTTGCTGG ATCACCATCA CAAGCGCATG 
AGCAACTTCA AGATGGCTGG TTCCTCTTTA GAAGCCTCTT CCAAGGTTTA CGGCCTGCGT 
GTGGACTCCA TCTACTTGGA CGCCATGCGC ATTTCAGCTG GCTTGAGTGC ACGAACTCTT 
ACGGATAAGC AAATAAATGC TGCCGAGGAT GATGATGGCC CCCAAGGTGA ACAGGCTACT 
GGCGAGGGTC AGGACTCAGC GCAACAGGCA GCAAAAGAAG CAGCACCGAA GCCCAAACGG 
CAAAAGAAAC CCATATCCAC CGTGACCAAA AACAGAGAAA CGCTGAACAG CCGGCTGGAT 
ACGGCTCCCT TGCAGGATCC TGTGTTTGGC AAGCTGAACT CCACTGTGGG CTCTATAAAT 
GCTTCCAATC GTCTGATGCA CAACATTCTT CCTAGTTTCG ACTCTGAATT GCGCTTGCGT 
ACCACCTACA ATTTTTGGAA CAGCGAAGAG TCCACCGAGG AGGTCCAGGA CCACACTACA 
CTCAATGCAG AAATGGAACA ATGGCCAGCC ACATCACTTA TGAGCACCAA TCTTATGCGC 
AAACTGCTGC CACATGCTGA AAGGTCAAAT CTGCGACCGC TGCATACGGG CTACATCATC 
ACAAGTGCTC CCAATCCCAA GTCGGCAAAT GAAAAGGCCG CCGAGGTAGT GCAGGATGAA 
GATCATGACG AGGGTCTGGA TAATGCCGAC GATATGTGTG TAAATGAGAT TTCCATGGCC 
TTTGACATCA ATGCCGAGTG TGAGCCCATG CCGGACCTGG ATGGACCACC GCCTCTGGTG 
CTGGAGGTGG ACTCCAATGA GCTGGAAGAG CTCACTGCCG AGGAGCAAAT GGTGATCAAT 
AATTGCCGTC GGCTACGTAA GCAGACAGAG TTCATCGAGG ATTTGCGACC GGTAGACGGG 
AATTCCAAGC TAGAGTACTC CTACAGGCCG ATGGACCAGA TCTCCCAGTT TTGGGCCGGC 
CCCTCGCATT GGAAGTTTAA GCGGACTCGC CCGCGCAGCA CTTTCTCGCA GACCAATGGA 
CAGGTGGACA CTCAGCCTAT TCGCACTCAA CGAGCGAAGA AGTCAGCGCA TCTGAATGCC 
AACCGAAGAG CCAAGGCTTT GGACTATGGC AATGTGACGG AAAACTTCTT CCAACAGCTG 
GACACCACCA TAAGGCAGCG CAAGGCGAAT TTCCAAAAGA AGTGGGATCC CCGAAAGCTG 
ATATTGCCCA CCAAGTTCGA ATTGGACCCG GATCTTTTCT TCAAGTACGA ATCCGCTCCC 
AGCATAAAGT TGTCGAAACG CGCAGGTGAA CCTGATTCGG ATGAGGGTGG AGATCTTGGC 
ATCGATATGG ATGCGGATAT GCACCACGAT GATGACAATG ACCAGGAGCT ATTCAACAAC 
GAGCATTTTA CCGACGCCGT TCCCGCCAAC GTGAGCGTTA TTGCCGCGAT TGCTGCGGAG 
CAAGCGGCCG AGGCAAGCAT GATGAACGTG TCCGCGGGTG AGATTGGCCT CACCCAGATG 
AACGCCACTT GCAACAACAC AGTCTTCGAG ATCGGCACCG AGTTCGAGGG TGCGCCGTCG 
CAGGTTGCCA AAGTGATTGT TCCATTCGCG AAGAGAGCCA AGGTTATAGA CATGAAGAAT 
CTGAAAAAGT CCTGCAACTC TCTAATACAG AAACAATTGC TAAACGCTGT GCCGGAGGAG 
ACGATACCCT CGCATCCCAA GAAAAAGGGC GAACACTATT CAAAGGGTTT CGCGAGTTTT 
CAGCAGGTGT ATCAGAAGCT GCCCGACCTT TTGACCACCA AAATGTCAGA CTCGCTGTCC 
CCGTCGGTGG CATTCTATGC GGTTCTGCAT TTGGCCAACG ATTTGAAACT GCGGCTAATT 
CCGCAGGAGG ACTTGGAGGA CTTTCAAATT CGCCAGGTGT TGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724240.1
CDS	255..2462
Translation	MTLPRLETPLRRSAVGSYQEGVSRMLTPFNDDEAERREARRRTLLQQHHRSSTLESIEDNETIKNCLELYNGNKVSKDNAWNLMLIDSLANLLDHHHKRMSNFKMAGSSLEASSKVYGLRVDSIYLDAMRISAGLSARTLTDKQINAAEDDDGPQGEQATGEGQDSAQQAAKEAAPKPKRQKKPISTVTKNRETLNSRLDTAPLQDPVFGKLNSTVGSINASNRLMHNILPSFDSELRLRTTYNFWNSEESTEEVQDHTTLNAEMEQWPATSLMSTNLMRKLLPHAERSNLRPLHTGYIITSAPNPKSANEKAAEVVQDEDHDEGLDNADDMCVNEISMAFDINAECEPMPDLDGPPPLVLEVDSNELEELTAEEQMVINNCRRLRKQTEFIEDLRPVDGNSKLEYSYRPMDQISQFWAGPSHWKFKRTRPRSTFSQTNGQVDTQPIRTQRAKKSAHLNANRRAKALDYGNVTENFFQQLDTTIRQRKANFQKKWDPRKLILPTKFELDPDLFFKYESAPSIKLSKRAGEPDSDEGGDLGIDMDADMHHDDDNDQELFNNEHFTDAVPANVSVIAAIAAEQAAEASMMNVSAGEIGLTQMNATCNNTVFEIGTEFEGAPSQVAKVIVPFAKRAKVIDMKNLKKSCNSLIQKQLLNAVPEETIPSHPKKKGEHYSKGFASFQQVYQKLPDLLTTKMSDSLSPSVAFYAVLHLANDLKLRLIPQEDLEDFQIRQVLD

Target ORF information:

RefSeq Version	NM_165317.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster barren (barr), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165317.3

ORF Insert Sequence:

ATGACTCTGC CCCGCTTAGA AACTCCGCTC AGGCGGTCCG CAGTAGGCAG TTACCAGGAG 
GGGGTGTCCC GCATGCTAAC CCCCTTTAAC GACGACGAGG CGGAAAGGCG GGAAGCCCGA 
CGTCGCACGC TCCTTCAGCA GCATCACCGG TCGAGCACTC TGGAGTCCAT CGAGGACAAT 
GAGACCATCA AAAATTGCTT GGAGCTCTAC AATGGCAACA AGGTGAGCAA GGACAACGCC 
TGGAACCTGA TGCTCATCGA TTCGTTGGCC AATTTGCTGG ATCACCATCA CAAGCGCATG 
AGCAACTTCA AGATGGCTGG TTCCTCTTTA GAAGCCTCTT CCAAGGTTTA CGGCCTGCGT 
GTGGACTCCA TCTACTTGGA CGCCATGCGC ATTTCAGCTG GCTTGAGTGC ACGAACTCTT 
ACGGATAAGC AAATAAATGC TGCCGAGGAT GATGATGGCC CCCAAGGTGA ACAGGCTACT 
GGCGAGGGTC AGGACTCAGC GCAACAGGCA GCAAAAGAAG CAGCACCGAA GCCCAAACGG 
CAAAAGAAAC CCATATCCAC CGTGACCAAA AACAGAGAAA CGCTGAACAG CCGGCTGGAT 
ACGGCTCCCT TGCAGGATCC TGTGTTTGGC AAGCTGAACT CCACTGTGGG CTCTATAAAT 
GCTTCCAATC GTCTGATGCA CAACATTCTT CCTAGTTTCG ACTCTGAATT GCGCTTGCGT 
ACCACCTACA ATTTTTGGAA CAGCGAAGAG TCCACCGAGG AGGTCCAGGA CCACACTACA 
CTCAATGCAG AAATGGAACA ATGGCCAGCC ACATCACTTA TGAGCACCAA TCTTATGCGC 
AAACTGCTGC CACATGCTGA AAGGTCAAAT CTGCGACCGC TGCATACGGG CTACATCATC 
ACAAGTGCTC CCAATCCCAA GTCGGCAAAT GAAAAGGCCG CCGAGGTAGT GCAGGATGAA 
GATCATGACG AGGGTCTGGA TAATGCCGAC GATATGTGTG TAAATGAGAT TTCCATGGCC 
TTTGACATCA ATGCCGAGTG TGAGCCCATG CCGGACCTGG ATGGACCACC GCCTCTGGTG 
CTGGAGGTGG ACTCCAATGA GCTGGAAGAG CTCACTGCCG AGGAGCAAAT GGTGATCAAT 
AATTGCCGTC GGCTACGTAA GCAGACAGAG TTCATCGAGG ATTTGCGACC GGTAGACGGG 
AATTCCAAGC TAGAGTACTC CTACAGGCCG ATGGACCAGA TCTCCCAGTT TTGGGCCGGC 
CCCTCGCATT GGAAGTTTAA GCGGACTCGC CCGCGCAGCA CTTTCTCGCA GACCAATGGA 
CAGGTGGACA CTCAGCCTAT TCGCACTCAA CGAGCGAAGA AGTCAGCGCA TCTGAATGCC 
AACCGAAGAG CCAAGGCTTT GGACTATGGC AATGTGACGG AAAACTTCTT CCAACAGCTG 
GACACCACCA TAAGGCAGCG CAAGGCGAAT TTCCAAAAGA AGTGGGATCC CCGAAAGCTG 
ATATTGCCCA CCAAGTTCGA ATTGGACCCG GATCTTTTCT TCAAGTACGA ATCCGCTCCC 
AGCATAAAGT TGTCGAAACG CGCAGGTGAA CCTGATTCGG ATGAGGGTGG AGATCTTGGC 
ATCGATATGG ATGCGGATAT GCACCACGAT GATGACAATG ACCAGGAGCT ATTCAACAAC 
GAGCATTTTA CCGACGCCGT TCCCGCCAAC GTGAGCGTTA TTGCCGCGAT TGCTGCGGAG 
CAAGCGGCCG AGGCAAGCAT GATGAACGTG TCCGCGGGTG AGATTGGCCT CACCCAGATG 
AACGCCACTT GCAACAACAC AGTCTTCGAG ATCGGCACCG AGTTCGAGGG TGCGCCGTCG 
CAGGTTGCCA AAGTGATTGT TCCATTCGCG AAGAGAGCCA AGGTTATAGA CATGAAGAAT 
CTGAAAAAGT CCTGCAACTC TCTAATACAG AAACAATTGC TAAACGCTGT GCCGGAGGAG 
ACGATACCCT CGCATCCCAA GAAAAAGGGC GAACACTATT CAAAGGGTTT CGCGAGTTTT 
CAGCAGGTGT ATCAGAAGCT GCCCGACCTT TTGACCACCA AAATGTCAGA CTCGCTGTCC 
CCGTCGGTGG CATTCTATGC GGTTCTGCAT TTGGCCAACG ATTTGAAACT GCGGCTAATT 
CCGCAGGAGG ACTTGGAGGA CTTTCAAATT CGCCAGGTGT TGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

barr ( NM_001299165.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286094.1 Drosophila melanogaster barren (barr), transcript variant C, mRNA.

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CloneID	OFa16925
Clone ID Related Accession (Same CDS sequence)	NM_057758.5 , NM_165317.3 , NM_001299165.1
Accession Version	NM_001299165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	barren, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTCTGC CCCGCTTAGA AACTCCGCTC AGGCGGTCCG CAGTAGGCAG TTACCAGGAG 
GGGGTGTCCC GCATGCTAAC CCCCTTTAAC GACGACGAGG CGGAAAGGCG GGAAGCCCGA 
CGTCGCACGC TCCTTCAGCA GCATCACCGG TCGAGCACTC TGGAGTCCAT CGAGGACAAT 
GAGACCATCA AAAATTGCTT GGAGCTCTAC AATGGCAACA AGGTGAGCAA GGACAACGCC 
TGGAACCTGA TGCTCATCGA TTCGTTGGCC AATTTGCTGG ATCACCATCA CAAGCGCATG 
AGCAACTTCA AGATGGCTGG TTCCTCTTTA GAAGCCTCTT CCAAGGTTTA CGGCCTGCGT 
GTGGACTCCA TCTACTTGGA CGCCATGCGC ATTTCAGCTG GCTTGAGTGC ACGAACTCTT 
ACGGATAAGC AAATAAATGC TGCCGAGGAT GATGATGGCC CCCAAGGTGA ACAGGCTACT 
GGCGAGGGTC AGGACTCAGC GCAACAGGCA GCAAAAGAAG CAGCACCGAA GCCCAAACGG 
CAAAAGAAAC CCATATCCAC CGTGACCAAA AACAGAGAAA CGCTGAACAG CCGGCTGGAT 
ACGGCTCCCT TGCAGGATCC TGTGTTTGGC AAGCTGAACT CCACTGTGGG CTCTATAAAT 
GCTTCCAATC GTCTGATGCA CAACATTCTT CCTAGTTTCG ACTCTGAATT GCGCTTGCGT 
ACCACCTACA ATTTTTGGAA CAGCGAAGAG TCCACCGAGG AGGTCCAGGA CCACACTACA 
CTCAATGCAG AAATGGAACA ATGGCCAGCC ACATCACTTA TGAGCACCAA TCTTATGCGC 
AAACTGCTGC CACATGCTGA AAGGTCAAAT CTGCGACCGC TGCATACGGG CTACATCATC 
ACAAGTGCTC CCAATCCCAA GTCGGCAAAT GAAAAGGCCG CCGAGGTAGT GCAGGATGAA 
GATCATGACG AGGGTCTGGA TAATGCCGAC GATATGTGTG TAAATGAGAT TTCCATGGCC 
TTTGACATCA ATGCCGAGTG TGAGCCCATG CCGGACCTGG ATGGACCACC GCCTCTGGTG 
CTGGAGGTGG ACTCCAATGA GCTGGAAGAG CTCACTGCCG AGGAGCAAAT GGTGATCAAT 
AATTGCCGTC GGCTACGTAA GCAGACAGAG TTCATCGAGG ATTTGCGACC GGTAGACGGG 
AATTCCAAGC TAGAGTACTC CTACAGGCCG ATGGACCAGA TCTCCCAGTT TTGGGCCGGC 
CCCTCGCATT GGAAGTTTAA GCGGACTCGC CCGCGCAGCA CTTTCTCGCA GACCAATGGA 
CAGGTGGACA CTCAGCCTAT TCGCACTCAA CGAGCGAAGA AGTCAGCGCA TCTGAATGCC 
AACCGAAGAG CCAAGGCTTT GGACTATGGC AATGTGACGG AAAACTTCTT CCAACAGCTG 
GACACCACCA TAAGGCAGCG CAAGGCGAAT TTCCAAAAGA AGTGGGATCC CCGAAAGCTG 
ATATTGCCCA CCAAGTTCGA ATTGGACCCG GATCTTTTCT TCAAGTACGA ATCCGCTCCC 
AGCATAAAGT TGTCGAAACG CGCAGGTGAA CCTGATTCGG ATGAGGGTGG AGATCTTGGC 
ATCGATATGG ATGCGGATAT GCACCACGAT GATGACAATG ACCAGGAGCT ATTCAACAAC 
GAGCATTTTA CCGACGCCGT TCCCGCCAAC GTGAGCGTTA TTGCCGCGAT TGCTGCGGAG 
CAAGCGGCCG AGGCAAGCAT GATGAACGTG TCCGCGGGTG AGATTGGCCT CACCCAGATG 
AACGCCACTT GCAACAACAC AGTCTTCGAG ATCGGCACCG AGTTCGAGGG TGCGCCGTCG 
CAGGTTGCCA AAGTGATTGT TCCATTCGCG AAGAGAGCCA AGGTTATAGA CATGAAGAAT 
CTGAAAAAGT CCTGCAACTC TCTAATACAG AAACAATTGC TAAACGCTGT GCCGGAGGAG 
ACGATACCCT CGCATCCCAA GAAAAAGGGC GAACACTATT CAAAGGGTTT CGCGAGTTTT 
CAGCAGGTGT ATCAGAAGCT GCCCGACCTT TTGACCACCA AAATGTCAGA CTCGCTGTCC 
CCGTCGGTGG CATTCTATGC GGTTCTGCAT TTGGCCAACG ATTTGAAACT GCGGCTAATT 
CCGCAGGAGG ACTTGGAGGA CTTTCAAATT CGCCAGGTGT TGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286094.1
CDS	479..2686
Translation	MTLPRLETPLRRSAVGSYQEGVSRMLTPFNDDEAERREARRRTLLQQHHRSSTLESIEDNETIKNCLELYNGNKVSKDNAWNLMLIDSLANLLDHHHKRMSNFKMAGSSLEASSKVYGLRVDSIYLDAMRISAGLSARTLTDKQINAAEDDDGPQGEQATGEGQDSAQQAAKEAAPKPKRQKKPISTVTKNRETLNSRLDTAPLQDPVFGKLNSTVGSINASNRLMHNILPSFDSELRLRTTYNFWNSEESTEEVQDHTTLNAEMEQWPATSLMSTNLMRKLLPHAERSNLRPLHTGYIITSAPNPKSANEKAAEVVQDEDHDEGLDNADDMCVNEISMAFDINAECEPMPDLDGPPPLVLEVDSNELEELTAEEQMVINNCRRLRKQTEFIEDLRPVDGNSKLEYSYRPMDQISQFWAGPSHWKFKRTRPRSTFSQTNGQVDTQPIRTQRAKKSAHLNANRRAKALDYGNVTENFFQQLDTTIRQRKANFQKKWDPRKLILPTKFELDPDLFFKYESAPSIKLSKRAGEPDSDEGGDLGIDMDADMHHDDDNDQELFNNEHFTDAVPANVSVIAAIAAEQAAEASMMNVSAGEIGLTQMNATCNNTVFEIGTEFEGAPSQVAKVIVPFAKRAKVIDMKNLKKSCNSLIQKQLLNAVPEETIPSHPKKKGEHYSKGFASFQQVYQKLPDLLTTKMSDSLSPSVAFYAVLHLANDLKLRLIPQEDLEDFQIRQVLD

Target ORF information:

RefSeq Version	NM_001299165.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster barren (barr), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299165.1

ORF Insert Sequence:

ATGACTCTGC CCCGCTTAGA AACTCCGCTC AGGCGGTCCG CAGTAGGCAG TTACCAGGAG 
GGGGTGTCCC GCATGCTAAC CCCCTTTAAC GACGACGAGG CGGAAAGGCG GGAAGCCCGA 
CGTCGCACGC TCCTTCAGCA GCATCACCGG TCGAGCACTC TGGAGTCCAT CGAGGACAAT 
GAGACCATCA AAAATTGCTT GGAGCTCTAC AATGGCAACA AGGTGAGCAA GGACAACGCC 
TGGAACCTGA TGCTCATCGA TTCGTTGGCC AATTTGCTGG ATCACCATCA CAAGCGCATG 
AGCAACTTCA AGATGGCTGG TTCCTCTTTA GAAGCCTCTT CCAAGGTTTA CGGCCTGCGT 
GTGGACTCCA TCTACTTGGA CGCCATGCGC ATTTCAGCTG GCTTGAGTGC ACGAACTCTT 
ACGGATAAGC AAATAAATGC TGCCGAGGAT GATGATGGCC CCCAAGGTGA ACAGGCTACT 
GGCGAGGGTC AGGACTCAGC GCAACAGGCA GCAAAAGAAG CAGCACCGAA GCCCAAACGG 
CAAAAGAAAC CCATATCCAC CGTGACCAAA AACAGAGAAA CGCTGAACAG CCGGCTGGAT 
ACGGCTCCCT TGCAGGATCC TGTGTTTGGC AAGCTGAACT CCACTGTGGG CTCTATAAAT 
GCTTCCAATC GTCTGATGCA CAACATTCTT CCTAGTTTCG ACTCTGAATT GCGCTTGCGT 
ACCACCTACA ATTTTTGGAA CAGCGAAGAG TCCACCGAGG AGGTCCAGGA CCACACTACA 
CTCAATGCAG AAATGGAACA ATGGCCAGCC ACATCACTTA TGAGCACCAA TCTTATGCGC 
AAACTGCTGC CACATGCTGA AAGGTCAAAT CTGCGACCGC TGCATACGGG CTACATCATC 
ACAAGTGCTC CCAATCCCAA GTCGGCAAAT GAAAAGGCCG CCGAGGTAGT GCAGGATGAA 
GATCATGACG AGGGTCTGGA TAATGCCGAC GATATGTGTG TAAATGAGAT TTCCATGGCC 
TTTGACATCA ATGCCGAGTG TGAGCCCATG CCGGACCTGG ATGGACCACC GCCTCTGGTG 
CTGGAGGTGG ACTCCAATGA GCTGGAAGAG CTCACTGCCG AGGAGCAAAT GGTGATCAAT 
AATTGCCGTC GGCTACGTAA GCAGACAGAG TTCATCGAGG ATTTGCGACC GGTAGACGGG 
AATTCCAAGC TAGAGTACTC CTACAGGCCG ATGGACCAGA TCTCCCAGTT TTGGGCCGGC 
CCCTCGCATT GGAAGTTTAA GCGGACTCGC CCGCGCAGCA CTTTCTCGCA GACCAATGGA 
CAGGTGGACA CTCAGCCTAT TCGCACTCAA CGAGCGAAGA AGTCAGCGCA TCTGAATGCC 
AACCGAAGAG CCAAGGCTTT GGACTATGGC AATGTGACGG AAAACTTCTT CCAACAGCTG 
GACACCACCA TAAGGCAGCG CAAGGCGAAT TTCCAAAAGA AGTGGGATCC CCGAAAGCTG 
ATATTGCCCA CCAAGTTCGA ATTGGACCCG GATCTTTTCT TCAAGTACGA ATCCGCTCCC 
AGCATAAAGT TGTCGAAACG CGCAGGTGAA CCTGATTCGG ATGAGGGTGG AGATCTTGGC 
ATCGATATGG ATGCGGATAT GCACCACGAT GATGACAATG ACCAGGAGCT ATTCAACAAC 
GAGCATTTTA CCGACGCCGT TCCCGCCAAC GTGAGCGTTA TTGCCGCGAT TGCTGCGGAG 
CAAGCGGCCG AGGCAAGCAT GATGAACGTG TCCGCGGGTG AGATTGGCCT CACCCAGATG 
AACGCCACTT GCAACAACAC AGTCTTCGAG ATCGGCACCG AGTTCGAGGG TGCGCCGTCG 
CAGGTTGCCA AAGTGATTGT TCCATTCGCG AAGAGAGCCA AGGTTATAGA CATGAAGAAT 
CTGAAAAAGT CCTGCAACTC TCTAATACAG AAACAATTGC TAAACGCTGT GCCGGAGGAG 
ACGATACCCT CGCATCCCAA GAAAAAGGGC GAACACTATT CAAAGGGTTT CGCGAGTTTT 
CAGCAGGTGT ATCAGAAGCT GCCCGACCTT TTGACCACCA AAATGTCAGA CTCGCTGTCC 
CCGTCGGTGG CATTCTATGC GGTTCTGCAT TTGGCCAACG ATTTGAAACT GCGGCTAATT 
CCGCAGGAGG ACTTGGAGGA CTTTCAAATT CGCCAGGTGT TGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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barr cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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