Hakai cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hakai
Entrez Gene ID	35256
Full Name	CG10263 gene product from transcript CG10263-RE
Synonyms	CG10263,Dmel\CG10263,hakai
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37F2-37F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_165312.2	NP_724217.1	hakai, isoform C
NM_176061.3	NP_788075.2	hakai, isoform E
NM_001273664.1	NP_001260593.1	hakai, isoform F
NM_136149.3	NP_609993.1	hakai, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hakai gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hakai cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14998	NM_001273664.1 Latest version!	Drosophila melanogaster hakai, transcript variant F (Hakai), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa16897	NM_136149.3 Latest version!	Drosophila melanogaster hakai, transcript variant A (Hakai), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa16899	NM_165312.2 Latest version!	Drosophila melanogaster hakai, transcript variant C (Hakai), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa16936	NM_176061.3 Latest version!	Drosophila melanogaster hakai, transcript variant E (Hakai), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hakai ( NM_001273664.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260593.1 Drosophila melanogaster hakai, transcript variant F (Hakai), mRNA.

Price & Availability↑

CloneID	OFa14998
Clone ID Related Accession (Same CDS sequence)	NM_001273664.1
Accession Version	NM_001273664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hakai, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACCG AGGAAGTGAA GCGCGGCCGC GGACGAGGGC GTGGCACCAG GGCTCGCGGG 
CGCGGACGTG GCCGAGGACG CGGTCGTGGC AAGAAAATCG ACGACAGCAG CATCGCCGAC 
GCCGCAGCCC TGGCGGCCAG CAGCTGTGCA GCCCTGGAGG ACAGTCCGGG AAGACTGGAC 
GCCTCCGAAG ATTCGGTCAT GCAGGAGCTC GACAAGGACG GCGAACTGGA AACGCCCGGC 
GCTCTGGAGG AGCCCCTGCC GCACGGTGCG CTAGGAGCGG TCGCCGCCAG CGGGAACATG 
ACTCCAGCGA CGCAACAGCC ACAGGTCCTG CAGCAAGTGC CACCGCCCGT GATGAGCCAA 
ACAACTATCT CGCTATCTCT CGCCCGCGCA GTCGACATGG AGGCGGACAT ATCGCAACTG 
GAGGCGCCCA CTTTCACCAC GCTTTCGCGC GGACCGCCGG AGCCGATGCT GCGCCTTAAG 
TGGAACCACA AGGTGAGCCT GATCGGCGAG AAGGTGCTGA ACCCGATGAT CCACTGCTGC 
GATCAGTGCG ACAAGCCGAT CCTCGTCTAC GGCCGCATGA TACCCTGCAA GCATGTATTT 
TGCCTCAAGT GCGCCCGCGC CGAGCCCATC AAGAGTTGTC CCCGCTGCAC GGACAAGGTG 
TTGCGCGTGG AGCAGAGTGG CCTGGGCACC GTGTTCATGT GCACGCACGG CGGCAGTCGC 
TACGGCAGCT CCGGCTGCCG TAGAACATAC CTTTCGCAAC GCGACCTGCA GGCGCACATC 
AATCACAGAC ATGTGGCGCC GCAGCCGCCA CCGTTGCAGC CACAGCCGCA GCTGTCAGCC 
ATGGCGGAGC AGCCAAAAAT GACGGATCTG GGCGGCGTCG GTCTGGGCCT GGAGTTGCAC 
AAGCAGCGCA AGCTCTCCGA GTCGTCTGTC CCCATATCCG TTTCCGCCTC CATTGCGTCC 
CGTCCCGTCC TGTCGCGGCT GCCACTGACC GGCGGTGTCG GTAACATCGG TAGTATTGGT 
AGTATACCGC CACCAGGATC CGCCGCCGCC GCCCAAAACG CCATCCACGG CGGCCACTCG 
ACGCTCACGC TGGCCAATTT GACCAGGATC AACAATGCCA ATGCGCAGGA ATGTCACCAG 
GGCAAGGCCT CGCTGCACCA CACGCTCAAG AAGGGCACGC CCCACCAGTC CGAGTCGGTG 
GCGGATGCCT CCTACTACAG CAGCGTGCTC GCCTCCTTTG GCAGTGCGGC CGGGAATCCG 
GGTTCTGGGC CACCCGGCGG TGGGGCAACA GCAGCAGCAC AGCCCGCCAA TCCGAGTGGC 
AGCCATTCCG CTGTTGGCCC AGGTGCCCTG ATTGGAGGCT CCACCGACGC GCCAACTGGT 
GGCTCGAGTG GCAACTGGCA GCAGTCGCAA TACTACAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260593.1
CDS	105..1526
Translation	MDTEEVKRGRGRGRGTRARGRGRGRGRGRGKKIDDSSIADAAALAASSCAALEDSPGRLDASEDSVMQELDKDGELETPGALEEPLPHGALGAVAASGNMTPATQQPQVLQQVPPPVMSQTTISLSLARAVDMEADISQLEAPTFTTLSRGPPEPMLRLKWNHKVSLIGEKVLNPMIHCCDQCDKPILVYGRMIPCKHVFCLKCARAEPIKSCPRCTDKVLRVEQSGLGTVFMCTHGGSRYGSSGCRRTYLSQRDLQAHINHRHVAPQPPPLQPQPQLSAMAEQPKMTDLGGVGLGLELHKQRKLSESSVPISVSASIASRPVLSRLPLTGGVGNIGSIGSIPPPGSAAAAQNAIHGGHSTLTLANLTRINNANAQECHQGKASLHHTLKKGTPHQSESVADASYYSSVLASFGSAAGNPGSGPPGGGATAAAQPANPSGSHSAVGPGALIGGSTDAPTGGSSGNWQQSQYYR

Target ORF information:

RefSeq Version	NM_001273664.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hakai, transcript variant F (Hakai), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273664.1

ORF Insert Sequence:

ATGGACACCG AGGAAGTGAA GCGCGGCCGC GGACGAGGGC GTGGCACCAG GGCTCGCGGG 
CGCGGACGTG GCCGAGGACG CGGTCGTGGC AAGAAAATCG ACGACAGCAG CATCGCCGAC 
GCCGCAGCCC TGGCGGCCAG CAGCTGTGCA GCCCTGGAGG ACAGTCCGGG AAGACTGGAC 
GCCTCCGAAG ATTCGGTCAT GCAGGAGCTC GACAAGGACG GCGAACTGGA AACGCCCGGC 
GCTCTGGAGG AGCCCCTGCC GCACGGTGCG CTAGGAGCGG TCGCCGCCAG CGGGAACATG 
ACTCCAGCGA CGCAACAGCC ACAGGTCCTG CAGCAAGTGC CACCGCCCGT GATGAGCCAA 
ACAACTATCT CGCTATCTCT CGCCCGCGCA GTCGACATGG AGGCGGACAT ATCGCAACTG 
GAGGCGCCCA CTTTCACCAC GCTTTCGCGC GGACCGCCGG AGCCGATGCT GCGCCTTAAG 
TGGAACCACA AGGTGAGCCT GATCGGCGAG AAGGTGCTGA ACCCGATGAT CCACTGCTGC 
GATCAGTGCG ACAAGCCGAT CCTCGTCTAC GGCCGCATGA TACCCTGCAA GCATGTATTT 
TGCCTCAAGT GCGCCCGCGC CGAGCCCATC AAGAGTTGTC CCCGCTGCAC GGACAAGGTG 
TTGCGCGTGG AGCAGAGTGG CCTGGGCACC GTGTTCATGT GCACGCACGG CGGCAGTCGC 
TACGGCAGCT CCGGCTGCCG TAGAACATAC CTTTCGCAAC GCGACCTGCA GGCGCACATC 
AATCACAGAC ATGTGGCGCC GCAGCCGCCA CCGTTGCAGC CACAGCCGCA GCTGTCAGCC 
ATGGCGGAGC AGCCAAAAAT GACGGATCTG GGCGGCGTCG GTCTGGGCCT GGAGTTGCAC 
AAGCAGCGCA AGCTCTCCGA GTCGTCTGTC CCCATATCCG TTTCCGCCTC CATTGCGTCC 
CGTCCCGTCC TGTCGCGGCT GCCACTGACC GGCGGTGTCG GTAACATCGG TAGTATTGGT 
AGTATACCGC CACCAGGATC CGCCGCCGCC GCCCAAAACG CCATCCACGG CGGCCACTCG 
ACGCTCACGC TGGCCAATTT GACCAGGATC AACAATGCCA ATGCGCAGGA ATGTCACCAG 
GGCAAGGCCT CGCTGCACCA CACGCTCAAG AAGGGCACGC CCCACCAGTC CGAGTCGGTG 
GCGGATGCCT CCTACTACAG CAGCGTGCTC GCCTCCTTTG GCAGTGCGGC CGGGAATCCG 
GGTTCTGGGC CACCCGGCGG TGGGGCAACA GCAGCAGCAC AGCCCGCCAA TCCGAGTGGC 
AGCCATTCCG CTGTTGGCCC AGGTGCCCTG ATTGGAGGCT CCACCGACGC GCCAACTGGT 
GGCTCGAGTG GCAACTGGCA GCAGTCGCAA TACTACAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hakai ( NM_136149.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609993.1 Drosophila melanogaster hakai, transcript variant A (Hakai), mRNA.

Price & Availability↑

CloneID	OFa16897
Clone ID Related Accession (Same CDS sequence)	NM_136149.3
Accession Version	NM_136149.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hakai, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_136149.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACCG AGGAAGTGAA GCGCGGCCGC GGACGAGGGC GTGGCACCAG GGCTCGCGGG 
CGCGGACGTG GCCGAGGACG CGGTCGTGGC AAGAAAATCG ACGACAGCAG CATCGCCGAC 
GCCGCAGCCC TGGCGGCCAG CAGCTGTGCA GCCCTGGAGG ACAGTCCGGG AAGACTGGAC 
GCCTCCGAAG ATTCGGTCAT GCAGGAGCTC GACAAGGACG GCGAACTGGA AACGCCCGGC 
GCTCTGGAGG AGCCCCTGCC GCACGGTGCG CTAGGAGCGG TCGCCGCCAG CGGGAACATG 
ACTCCAGCGA CGCAACAGCC ACAGGTCCTG CAGCAAGTGC CACCGCCCGT GATGAGCCAA 
ACAATCGACA TGGAGGCGGA CATATCGCAA CTGGAGGCGC CCACTTTCAC CACGCTTTCG 
CGCGGACCGC CGGAGCCGAT GCTGCGCCTT AAGTGGAACC ACAAGGTGAG CCTGATCGGC 
GAGAAGGTGC TGAACCCGAT GATCCACTGC TGCGATCAGT GCGACAAGCC GATCCTCGTC 
TACGGCCGCA TGATACCCTG CAAGCATGTA TTTTGCCTCA AGTGCGCCCG CGCCGAGCCC 
ATCAAGAGTT GTCCCCGCTG CACGGACAAG GTGTTGCGCG TGGAGCAGAG TGGCCTGGGC 
ACCGTGTTCA TGTGCACGCA CGGCGGCAGT CGCTACGGCA GCTCCGGCTG CCGTAGAACA 
TACCTTTCGC AACGCGACCT GCAGGCGCAC ATCAATCACA GACATGTGGC GCCGCAGCCG 
CCACCGTTGC AGCCACAGCC GCAGCTGTCA GCCATGGCGG AGCAGCCAAA AATGACGGAT 
CTGGGCGGCG TCGGTCTGGG CCTGGAGTTG CACAAGCAGC GCAAGGCGAG TGGTAGCTAT 
GGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609993.1
CDS	105..1013
Translation	MDTEEVKRGRGRGRGTRARGRGRGRGRGRGKKIDDSSIADAAALAASSCAALEDSPGRLDASEDSVMQELDKDGELETPGALEEPLPHGALGAVAASGNMTPATQQPQVLQQVPPPVMSQTIDMEADISQLEAPTFTTLSRGPPEPMLRLKWNHKVSLIGEKVLNPMIHCCDQCDKPILVYGRMIPCKHVFCLKCARAEPIKSCPRCTDKVLRVEQSGLGTVFMCTHGGSRYGSSGCRRTYLSQRDLQAHINHRHVAPQPPPLQPQPQLSAMAEQPKMTDLGGVGLGLELHKQRKASGSYGS

Target ORF information:

RefSeq Version	NM_136149.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hakai, transcript variant A (Hakai), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136149.3

ORF Insert Sequence:

ATGGACACCG AGGAAGTGAA GCGCGGCCGC GGACGAGGGC GTGGCACCAG GGCTCGCGGG 
CGCGGACGTG GCCGAGGACG CGGTCGTGGC AAGAAAATCG ACGACAGCAG CATCGCCGAC 
GCCGCAGCCC TGGCGGCCAG CAGCTGTGCA GCCCTGGAGG ACAGTCCGGG AAGACTGGAC 
GCCTCCGAAG ATTCGGTCAT GCAGGAGCTC GACAAGGACG GCGAACTGGA AACGCCCGGC 
GCTCTGGAGG AGCCCCTGCC GCACGGTGCG CTAGGAGCGG TCGCCGCCAG CGGGAACATG 
ACTCCAGCGA CGCAACAGCC ACAGGTCCTG CAGCAAGTGC CACCGCCCGT GATGAGCCAA 
ACAATCGACA TGGAGGCGGA CATATCGCAA CTGGAGGCGC CCACTTTCAC CACGCTTTCG 
CGCGGACCGC CGGAGCCGAT GCTGCGCCTT AAGTGGAACC ACAAGGTGAG CCTGATCGGC 
GAGAAGGTGC TGAACCCGAT GATCCACTGC TGCGATCAGT GCGACAAGCC GATCCTCGTC 
TACGGCCGCA TGATACCCTG CAAGCATGTA TTTTGCCTCA AGTGCGCCCG CGCCGAGCCC 
ATCAAGAGTT GTCCCCGCTG CACGGACAAG GTGTTGCGCG TGGAGCAGAG TGGCCTGGGC 
ACCGTGTTCA TGTGCACGCA CGGCGGCAGT CGCTACGGCA GCTCCGGCTG CCGTAGAACA 
TACCTTTCGC AACGCGACCT GCAGGCGCAC ATCAATCACA GACATGTGGC GCCGCAGCCG 
CCACCGTTGC AGCCACAGCC GCAGCTGTCA GCCATGGCGG AGCAGCCAAA AATGACGGAT 
CTGGGCGGCG TCGGTCTGGG CCTGGAGTTG CACAAGCAGC GCAAGGCGAG TGGTAGCTAT 
GGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hakai ( NM_165312.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724217.1 Drosophila melanogaster hakai, transcript variant C (Hakai), mRNA.

Price & Availability↑

CloneID	OFa16899
Clone ID Related Accession (Same CDS sequence)	NM_165312.2
Accession Version	NM_165312.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hakai, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165312.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACCG AGGAAGTGAA GCGCGGCCGC GGACGAGGGC GTGGCACCAG GGCTCGCGGG 
CGCGGACGTG GCCGAGGACG CGGTCGTGGC AAGAAAATCG ACGACAGCAG CATCGCCGAC 
GCCGCAGCCC TGGCGGCCAG CAGCTGTGCA GCCCTGGAGG ACAGTCCGGG AAGACTGGAC 
GCCTCCGAAG ATTCGGTCAT GCAGGAGCTC GACAAGGACG GCGAACTGGA AACGCCCGGC 
GCTCTGGAGG AGCCCCTGCC GCACGGTGCG CTAGGAGCGG TCGCCGCCAG CGGGAACATG 
ACTCCAGCGA CGCAACAGCC ACAGGTCCTG CAGCAAGTGC CACCGCCCGT GATGAGCCAA 
ACAACTATCT CGCTATCTCT CGCCCGCGCA GTCGACATGG AGGCGGACAT ATCGCAACTG 
GAGGCGCCCA CTTTCACCAC GCTTTCGCGC GGACCGCCGG AGCCGATGCT GCGCCTTAAG 
TGGAACCACA AGGTGAGCCT GATCGGCGAG AAGGTGCTGA ACCCGATGAT CCACTGCTGC 
GATCAGTGCG ACAAGCCGAT CCTCGTCTAC GGCCGCATGA TACCCTGCAA GCATGTATTT 
TGCCTCAAGT GCGCCCGCGC CGAGCCCATC AAGAGTTGTC CCCGCTGCAC GGACAAGGTG 
TTGCGCGTGG AGCAGAGTGG CCTGGGCACC GTGTTCATGT GCACGCACGG CGGCAGTCGC 
TACGGCAGCT CCGGCTGCCG TAGAACATAC CTTTCGCAAC GCGACCTGCA GGCGCACATC 
AATCACAGAC ATGTGGCGCC GCAGCCGCCA CCGTTGCAGC CACAGCCGCA GCTGTCAGCC 
ATGGCGGAGC AGCCAAAAAT GACGGATCTG GGCGGCGTCG GTCTGGGCCT GGAGTTGCAC 
AAGCAGCGCA AGGCGAGTGG TAGCTATGGC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724217.1
CDS	105..1040
Translation	MDTEEVKRGRGRGRGTRARGRGRGRGRGRGKKIDDSSIADAAALAASSCAALEDSPGRLDASEDSVMQELDKDGELETPGALEEPLPHGALGAVAASGNMTPATQQPQVLQQVPPPVMSQTTISLSLARAVDMEADISQLEAPTFTTLSRGPPEPMLRLKWNHKVSLIGEKVLNPMIHCCDQCDKPILVYGRMIPCKHVFCLKCARAEPIKSCPRCTDKVLRVEQSGLGTVFMCTHGGSRYGSSGCRRTYLSQRDLQAHINHRHVAPQPPPLQPQPQLSAMAEQPKMTDLGGVGLGLELHKQRKASGSYGS

Target ORF information:

RefSeq Version	NM_165312.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hakai, transcript variant C (Hakai), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165312.2

ORF Insert Sequence:

ATGGACACCG AGGAAGTGAA GCGCGGCCGC GGACGAGGGC GTGGCACCAG GGCTCGCGGG 
CGCGGACGTG GCCGAGGACG CGGTCGTGGC AAGAAAATCG ACGACAGCAG CATCGCCGAC 
GCCGCAGCCC TGGCGGCCAG CAGCTGTGCA GCCCTGGAGG ACAGTCCGGG AAGACTGGAC 
GCCTCCGAAG ATTCGGTCAT GCAGGAGCTC GACAAGGACG GCGAACTGGA AACGCCCGGC 
GCTCTGGAGG AGCCCCTGCC GCACGGTGCG CTAGGAGCGG TCGCCGCCAG CGGGAACATG 
ACTCCAGCGA CGCAACAGCC ACAGGTCCTG CAGCAAGTGC CACCGCCCGT GATGAGCCAA 
ACAACTATCT CGCTATCTCT CGCCCGCGCA GTCGACATGG AGGCGGACAT ATCGCAACTG 
GAGGCGCCCA CTTTCACCAC GCTTTCGCGC GGACCGCCGG AGCCGATGCT GCGCCTTAAG 
TGGAACCACA AGGTGAGCCT GATCGGCGAG AAGGTGCTGA ACCCGATGAT CCACTGCTGC 
GATCAGTGCG ACAAGCCGAT CCTCGTCTAC GGCCGCATGA TACCCTGCAA GCATGTATTT 
TGCCTCAAGT GCGCCCGCGC CGAGCCCATC AAGAGTTGTC CCCGCTGCAC GGACAAGGTG 
TTGCGCGTGG AGCAGAGTGG CCTGGGCACC GTGTTCATGT GCACGCACGG CGGCAGTCGC 
TACGGCAGCT CCGGCTGCCG TAGAACATAC CTTTCGCAAC GCGACCTGCA GGCGCACATC 
AATCACAGAC ATGTGGCGCC GCAGCCGCCA CCGTTGCAGC CACAGCCGCA GCTGTCAGCC 
ATGGCGGAGC AGCCAAAAAT GACGGATCTG GGCGGCGTCG GTCTGGGCCT GGAGTTGCAC 
AAGCAGCGCA AGGCGAGTGG TAGCTATGGC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hakai ( NM_176061.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788075.2 Drosophila melanogaster hakai, transcript variant E (Hakai), mRNA.

Price & Availability↑

CloneID	OFa16936
Clone ID Related Accession (Same CDS sequence)	NM_176061.3
Accession Version	NM_176061.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hakai, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_176061.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACCG AGGAAGTGAA GCGCGGCCGC GGACGAGGGC GTGGCACCAG GGCTCGCGGG 
CGCGGACGTG GCCGAGGACG CGGTCGTGGC AAGAAAATCG ACGACAGCAG CATCGCCGAC 
GCCGCAGCCC TGGCGGCCAG CAGCTGTGCA GCCCTGGAGG ACAGTCCGGG AAGACTGGAC 
GCCTCCGAAG ATTCGGTCAT GCAGGAGCTC GACAAGGACG GCGAACTGGA AACGCCCGGC 
GCTCTGGAGG AGCCCCTGCC GCACGGTGCG CTAGGAGCGG TCGCCGCCAG CGGGAACATG 
ACTCCAGCGA CGCAACAGCC ACAGGTCCTG CAGCAAGTGC CACCGCCCGT GATGAGCCAA 
ACAATCGACA TGGAGGCGGA CATATCGCAA CTGGAGGCGC CCACTTTCAC CACGCTTTCG 
CGCGGACCGC CGGAGCCGAT GCTGCGCCTT AAGTGGAACC ACAAGGTGAG CCTGATCGGC 
GAGAAGGTGC TGAACCCGAT GATCCACTGC TGCGATCAGT GCGACAAGCC GATCCTCGTC 
TACGGCCGCA TGATACCCTG CAAGCATGTA TTTTGCCTCA AGTGCGCCCG CGCCGAGCCC 
ATCAAGAGTT GTCCCCGCTG CACGGACAAG GTGTTGCGCG TGGAGCAGAG TGGCCTGGGC 
ACCGTGTTCA TGTGCACGCA CGGCGGCAGT CGCTACGGCA GCTCCGGCTG CCGTAGAACA 
TACCTTTCGC AACGCGACCT GCAGGCGCAC ATCAATCACA GACATGTGGC GCCGCAGCCG 
CCACCGTTGC AGCCACAGCC GCAGCTGTCA GCCATGGCGG AGCAGCCAAA AATGACGGAT 
CTGGGCGGCG TCGGTCTGGG CCTGGAGTTG CACAAGCAGC GCAAGCTCTC CGAGTCGTCT 
GTCCCCATAT CCGTTTCCGC CTCCATTGCG TCCCGTCCCG TCCTGTCGCG GCTGCCACTG 
ACCGGCGGTG TCGGTAACAT CGGTAGTATT GGTAGTATAC CGCCACCAGG ATCCGCCGCC 
GCCGCCCAAA ACGCCATCCA CGGCGGCCAC TCGACGCTCA CGCTGGCCAA TTTGACCAGG 
ATCAACAATG CCAATGCGCA GGAATGTCAC CAGGGCAAGG CCTCGCTGCA CCACACGCTC 
AAGAAGGGCA CGCCCCACCA GTCCGAGTCG GTGGCGGATG CCTCCTACTA CAGCAGCGTG 
CTCGCCTCCT TTGGCAGTGC GGCCGGGAAT CCGGGTTCTG GGCCACCCGG CGGTGGGGCA 
ACAGCAGCAG CACAGCCCGC CAATCCGAGT GGCAGCCATT CCGCTGTTGG CCCAGGTGCC 
CTGATTGGAG GCTCCACCGA CGCGCCAACT GGTGGCTCGA GTGGCAACTG GCAGCAGTCG 
CAATACTACA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788075.2
CDS	105..1499
Translation	MDTEEVKRGRGRGRGTRARGRGRGRGRGRGKKIDDSSIADAAALAASSCAALEDSPGRLDASEDSVMQELDKDGELETPGALEEPLPHGALGAVAASGNMTPATQQPQVLQQVPPPVMSQTIDMEADISQLEAPTFTTLSRGPPEPMLRLKWNHKVSLIGEKVLNPMIHCCDQCDKPILVYGRMIPCKHVFCLKCARAEPIKSCPRCTDKVLRVEQSGLGTVFMCTHGGSRYGSSGCRRTYLSQRDLQAHINHRHVAPQPPPLQPQPQLSAMAEQPKMTDLGGVGLGLELHKQRKLSESSVPISVSASIASRPVLSRLPLTGGVGNIGSIGSIPPPGSAAAAQNAIHGGHSTLTLANLTRINNANAQECHQGKASLHHTLKKGTPHQSESVADASYYSSVLASFGSAAGNPGSGPPGGGATAAAQPANPSGSHSAVGPGALIGGSTDAPTGGSSGNWQQSQYYR

Target ORF information:

RefSeq Version	NM_176061.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hakai, transcript variant E (Hakai), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176061.3

ORF Insert Sequence:

ATGGACACCG AGGAAGTGAA GCGCGGCCGC GGACGAGGGC GTGGCACCAG GGCTCGCGGG 
CGCGGACGTG GCCGAGGACG CGGTCGTGGC AAGAAAATCG ACGACAGCAG CATCGCCGAC 
GCCGCAGCCC TGGCGGCCAG CAGCTGTGCA GCCCTGGAGG ACAGTCCGGG AAGACTGGAC 
GCCTCCGAAG ATTCGGTCAT GCAGGAGCTC GACAAGGACG GCGAACTGGA AACGCCCGGC 
GCTCTGGAGG AGCCCCTGCC GCACGGTGCG CTAGGAGCGG TCGCCGCCAG CGGGAACATG 
ACTCCAGCGA CGCAACAGCC ACAGGTCCTG CAGCAAGTGC CACCGCCCGT GATGAGCCAA 
ACAATCGACA TGGAGGCGGA CATATCGCAA CTGGAGGCGC CCACTTTCAC CACGCTTTCG 
CGCGGACCGC CGGAGCCGAT GCTGCGCCTT AAGTGGAACC ACAAGGTGAG CCTGATCGGC 
GAGAAGGTGC TGAACCCGAT GATCCACTGC TGCGATCAGT GCGACAAGCC GATCCTCGTC 
TACGGCCGCA TGATACCCTG CAAGCATGTA TTTTGCCTCA AGTGCGCCCG CGCCGAGCCC 
ATCAAGAGTT GTCCCCGCTG CACGGACAAG GTGTTGCGCG TGGAGCAGAG TGGCCTGGGC 
ACCGTGTTCA TGTGCACGCA CGGCGGCAGT CGCTACGGCA GCTCCGGCTG CCGTAGAACA 
TACCTTTCGC AACGCGACCT GCAGGCGCAC ATCAATCACA GACATGTGGC GCCGCAGCCG 
CCACCGTTGC AGCCACAGCC GCAGCTGTCA GCCATGGCGG AGCAGCCAAA AATGACGGAT 
CTGGGCGGCG TCGGTCTGGG CCTGGAGTTG CACAAGCAGC GCAAGCTCTC CGAGTCGTCT 
GTCCCCATAT CCGTTTCCGC CTCCATTGCG TCCCGTCCCG TCCTGTCGCG GCTGCCACTG 
ACCGGCGGTG TCGGTAACAT CGGTAGTATT GGTAGTATAC CGCCACCAGG ATCCGCCGCC 
GCCGCCCAAA ACGCCATCCA CGGCGGCCAC TCGACGCTCA CGCTGGCCAA TTTGACCAGG 
ATCAACAATG CCAATGCGCA GGAATGTCAC CAGGGCAAGG CCTCGCTGCA CCACACGCTC 
AAGAAGGGCA CGCCCCACCA GTCCGAGTCG GTGGCGGATG CCTCCTACTA CAGCAGCGTG 
CTCGCCTCCT TTGGCAGTGC GGCCGGGAAT CCGGGTTCTG GGCCACCCGG CGGTGGGGCA 
ACAGCAGCAG CACAGCCCGC CAATCCGAGT GGCAGCCATT CCGCTGTTGG CCCAGGTGCC 
CTGATTGGAG GCTCCACCGA CGCGCCAACT GGTGGCTCGA GTGGCAACTG GCAGCAGTCG 
CAATACTACA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hakai cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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