Tep4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tep4
Entrez Gene ID	35248
Full Name	Thioester-containing protein 4
Synonyms	CG10363,CT29102,Dmel\CG10363,TEP IV,TEP4,TEP4mel,TEPIV,Tep IV,TepIV,dTEPIV,tep4,tepIV
General protein information	Preferred Names Thioester-containing protein 4 Names CG10363 gene product from transcript CG10363-RD CG10363-PA CG10363-PB CG10363-PC CG10363-PD TEP Tep4-PA Tep4-PB Tep4-PC Tep4-PD thioester containing protein IV thiolester containing protein IV
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37F1-37F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_078879.3	NP_523603.2	Thioester-containing protein 4, isoform A
NM_001273662.1	NP_001260591.1	Thioester-containing protein 4, isoform D
NM_001273660.1	NP_001260589.1	Thioester-containing protein 4, isoform B
NM_001273661.1	NP_001260590.1	Thioester-containing protein 4, isoform C

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mug1	NP_032671.2
Drosophila melanogaster (fruit fly)	Tep4	NP_001260590.1
Mus musculus (house mouse)	Mug2	NP_032672.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these findings generate novel insights into the immune role of TEP4 as a regulator and effector of the Drosophla melanogaster antibacterial immune response
Title: Thioester-Containing Protein-4 Regulates the Drosophila Immune Signaling and Function against the Pathogen Photorhabdus.

The following Tep4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tep4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14993	NM_078879.3 Latest version!	Drosophila melanogaster Thioester-containing protein 4 (Tep4), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa16894	NM_001273662.1 Latest version!	Drosophila melanogaster Thioester-containing protein 4 (Tep4), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa16895	NM_001273660.1 Latest version!	Drosophila melanogaster Thioester-containing protein 4 (Tep4), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa16896	NM_001273661.1 Latest version!	Drosophila melanogaster Thioester-containing protein 4 (Tep4), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tep4 ( NM_078879.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523603.2 Drosophila melanogaster Thioester-containing protein 4 (Tep4), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14993
Clone ID Related Accession (Same CDS sequence)	NM_078879.3
Accession Version	NM_078879.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Thioester-containing protein 4, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078879.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCGCG CAGACGCTTT TGCTGTCTCG CTTTGTGTGA TTTTGGCCTT GCTGCAGACG 
ATGGAACCGG TAAAAGCCGA GGGCAAGTAC ACCATTGTGG GTCCCGGAAC CATCCACTCC 
CACCGTGACT ACAATGTGGC CGTGGCTGTC CATCAGACCA AGGAGCCAGT CACCCTGAAA 
GTGGGCATCA CTGGACCCTC CTACAACAAA ACTGAGACCG TGGAACTAGC CACTGCCGGT 
GAGTTCAAGC AGATCACCTT CAAACTGCCC CCATTGGAGG CGGGTGAATA TAATCTGACG 
GCGGAGGGCG TCAAGGGACT GGAGTTCAAG AACTCCACCA AGCTGAACTG GGAGAATTTC 
AAGCCATACA TTAAAATCCA GACCGATAAG GGAAAGTACA AGCCCGGTGA TACTATCAAC 
TATCGTGTGA TCTTCCTGGA CGAGAACCTG CGGCCGGATA CCGCTAAGGA TGAAGTGGTG 
GTTTGGTTTG AGGACTCCAA AAGGAACCGC ATCAAGCAGG AGAAACACAT AAAGACCACC 
GGTGGCGTCT ACACTGGCAA ATTCGAGCTC TCCGAGTTCG CCACATTGGG CTCCTGGTCC 
CTTCATGTTC AGAATGGAGA TCAGCACCAC GACGGAGGTA TTTACTTTGG GGGTCGTAAA 
CAATTTGGAG GATTTGGTCA TCGCTGGCAT CGCTCGGATG AGCTCGTCAA CTTTGAGGTG 
GAGAAGTACG TGCTGCCCAA GTACTCCGTC AAAATGGATG CCACGCAGCA GGTTTCAGTG 
CGAGATGGCG AGTTCAATGT CGTCCTGAAG GCCAACTACA CCTATGGTAA GCCCGTGAAT 
GGTAAAGTTC TTGTCAATGT CCATCTGGAC TCGACGAGCA GTTGGGAGAA TGTGGACGGC 
AAGACAGTGC AAACGGACTA CCCCGGACAT TCTGTTGTTG GCACCGCCGA CATGGTCGGA 
GGCAAGGCCA AGTTGACCAT GGATCTCAAG GACTTCGCCA GTTATCTGCC GCACAAGACC 
TCTTCTTCAT ATGCCCAGAT TACGGCTACC GTGGAGGAAG ACTTCACCGG CGTGAAGCTC 
AATGAAACAG GCGGTGTGCA GCTGTACCCC TATCGGTATG AGATGTCCTG CACTGACTAC 
TCCAGTTGCT TCTCCTTCAA GCCCGACAAG GAACATGAAC TTAACTTCAA GATTACATAT 
GTCGACGGCT CACTAATTAC AGACACCAAG TCTGTTGTGA AGGCCAAGTT CACTGAAGGA 
ATTCGACGCA ATTATGCTTT CTATGCCTTT GGAACAGATC ATCAAGAACC GGAATTGCCA 
ACGATTGAGA AGAAAACGTT TGTGTTTGAA AGCCATTTGA ATGCGTCTGG TGTGGCGCCT 
TTCAAGGTGG TTCTTCCCGA TTTGCCGGAT ATCGCCAACT TTACTCGTTA CTATAGCATT 
GAGCTGGAGT TCGTCGATGA AAAACGAGAT CTGTACACCA CTTATCCCTA CAGGGAACCA 
AAACAGATTG AAAATCCTAG CTCTGAAGAG GAAAAGGAGT GGTTCAGGGC AGAAGTTCAG 
AGGCCCAAGG ACGTCTGGAA CCTCAAGATC GGGCAGGAGT ATCAAGTAAT CCTCAACTCC 
AGTCGTCCGC TCAAATACTT TGTGTACAAC ATTGTGGGAC GTGGAAACAT ATTGGAAACG 
AAACGTGTGG ACCTCGCGGA ACCCCAAACG ACCGTCAACG TAACCATTAA GCCCACTTTC 
CTCACTACTC CCTATGGTCG GGTGTACTTC TACTACGTGG ATGAGACAGG AGAGTTCCGA 
TATACCGAGG AAACATTCAG TGTGGAGGTC GAGCTGCAGA ACCAGATCGA AATCAAGGCT 
CCCGCGGAAG TTAAACCGGG TGCCGACGTT GCACTGGAGA TCAAGACGTC GCCAAAGTCA 
TTCGTTGGAT TGTTGGCCGT CGATCAGAGT GTCCTTCTGC TGGGCAGCAA CAACGATTTG 
AACAAGGAAT CCTTCAACTG GCGCCTAAAT GGCTATGACA CCTCAACTCC CTGGCAGGGT 
GGATACTCCT ACTACCCCGG AGAGCGAACT GGAGTCGTGA CTATGACCAA TGCCTATTTC 
TTCTATAATC GCACCGCCCC AGACTACAAT ATTCAAGGAT TTGGTGGAAG CTCTTTCGCC 
ATGAGAAAGA CTACAGTGGC ACATGATAGT CATGTATTCC ATTCCGGTGC TGGCGGACCA 
ACTCAGGCCG TGGGATTCTC CGCCGAAAGT GCGTCTGCAT CTGCAGCACC AGTAGTGCGA 
AAGAATTTCG CAGAGACCTG GATCTTTGCC GATATCGAGA GCACCGAGGA GGAGGTGTTC 
AAGTGGGTAA AGACCATACC CGATACGATC ACCAACTGGG TGGTCACCGG CTTTTCACTG 
CATCCCCAGA AGGGATTAGG TGTCACCAAT GATCAGACAA ATATCAAGAC ATTCCAGCCG 
TTCTTTGTCT CCGTTCGATT GCCATACTCC GTGAAGCGAG GTGAGGTGAT CAATGTACCG 
GCACTGGTCT TTAACTACCT GCCCAAGACA CTGGACGTGG AACTGACCCT GGACAACGAG 
GATCAAGAGT ATGACTTCGT GGATGCCTCG AATGAGGTTA TTGGTGACCA GAAGCGTACG 
CAGAACATCA GAGTGGGCGC CAACGAAGCG GCAGGAGCCT CATTTTTGAT CCGACCCAAG 
GTCATTGGAA ATATTTTGCT GAAATTCAAG GCCATCTCAC CGCTGGCCGG AGATGCTATA 
CACAAGCCGC TAAAGGTAGT GCCCGAAGGA ATTACCCAGT ACCAGAACAG GGCATTCTTT 
ATCAATCTTA AGGATACGGG TGAGTTTAAG AACACCTTCG AGCTGGAAGT GCCGGAGGAT 
GTGGTCCCCG ACTCCGAGCG TGTGGAATTC GGATTGGTGG GTGATCTCTT GGGACCAGTG 
GTCAAGAACC TTGAGAACCT GTTGCGATTG CCCAGCGGTT GCGGTGAGCA GACCATGTCC 
AAATTGGTGC CCAACTATCT GGTGAGGGAT TATCTGAAGA GCATCAAGAA ACTCACACCT 
GCATTGGACA CTCGCATCAA GAGGAATCTG CAGGACGGAT ACCAACATAT GTTGCACTAT 
CGCCACGACG ACGGCAGCTT CAGTTCCTTT GGGCCCACCA AATGGAGGCA GGAGGATCCA 
GTGCGCAATG GTTCCACCTG GCTGACGGCC TATGTGCTGC GCTCGTTTAG CAAGATCAAG 
GATATAATTG ACTTGGATGA GCAGATCCTG GCCAAGGGCT ACGAATTCCT GTTAACTCGA 
CAGGCAGAAA ATGGTAGCTT CACGGAACAC GGAGAGTATT TCTATAGCTC CCAGCGATCT 
CTATTGACAT TGACCGCAAA TTCCTTGCTC GCCCTGCTGG AGGAGGAGAA ACCGAACCAG 
GCTGCCATTG ACAAGGCGGT TGCCTATCTG AGCGCCAATA CTGCCGAGTC CATTGAGCTC 
CTGCCCAAAT CCATTGCCAT TTATGCCCTG CAAAAGGCCA AGGCGCCGGA AGCTGCCAAG 
CAGGTGGCCA GTCTTAAGTC CCTGGCCAAA CACGAGGACG ATCGCACCTG GTGGACCGAG 
GATCTGGATA AGCTGCGTGC CTCCAAGAAC TGCGGACGCT GGTGGTGTTG GATCTGGAGT 
CAGGATGTGG AGATCACATC ATATGCGCTG CTTTCCCTTC TGGATTCAGA CCAGGAGACA 
GCCGACTCTG TTCTGAACAC CGTTCGCTGG TTGATCGCCC AGCGTAATGG CTTCGGAGGA 
TTTGCCTCCA GCCAGGACAC GGTTGTCGGC CTCACCGCCC TCATTAAGTT CGCTGAGAAG 
TCGGGCTACG AGGCGGCCAA GTGGGAGGTG ACCGTTTCCA ACAAGGGAAA GCGTGAAAAG 
ACCGAGAAGC TCAACACATC GGAGGAAAAT GACCTGCTCC TCCAGACAGT CGAGTTCCCA 
CAGGGCACCA AGTCGCTGGA GTTTGAAGCC AAGGGAACTG GAGCTGCTAT GGTCCAGATA 
AGCTACCAAT ACAACTTGGT GGAGAAGGAG CCGAAGCCGA GCTTTAAGAT TCAGACCACC 
GTTTTGCCGG AATCGTCGCC AGCCAATTTG GAACTAAGCG TCTGCGTGGA CTACGTGGAG 
GAGGGCGAGT CCAAGGAATC CAACATGGCC ATTCTGGAGG TATCCCTGCC GTCTGGTTAT 
ACTGCTGATG AGGATAGCTT CGCCGACATC CGGAACATCG AACGTGTGCG GTTGGTGGAA 
ACCAAGAACG GTGACTCCGT GGTGGTCATA TACTTCGAGA ATCTGGCCAA GAACGAAGAA 
AAATGCATTC GCATAGAGGC GTACAGAACA CATGCGGTGG CCAACCAGAA GCCATCATCG 
GTGGTCCTCT ACGATTACTA TGACACGAAC AAGAAGGCCA CCGAATACTA CTCCATCAAG 
TCGAAGCTCT GCGACATTTG CGAGGGTGAC GACTGCAAGA GCAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523603.2
CDS	154..4644
Translation	MRRADAFAVSLCVILALLQTMEPVKAEGKYTIVGPGTIHSHRDYNVAVAVHQTKEPVTLKVGITGPSYNKTETVELATAGEFKQITFKLPPLEAGEYNLTAEGVKGLEFKNSTKLNWENFKPYIKIQTDKGKYKPGDTINYRVIFLDENLRPDTAKDEVVVWFEDSKRNRIKQEKHIKTTGGVYTGKFELSEFATLGSWSLHVQNGDQHHDGGIYFGGRKQFGGFGHRWHRSDELVNFEVEKYVLPKYSVKMDATQQVSVRDGEFNVVLKANYTYGKPVNGKVLVNVHLDSTSSWENVDGKTVQTDYPGHSVVGTADMVGGKAKLTMDLKDFASYLPHKTSSSYAQITATVEEDFTGVKLNETGGVQLYPYRYEMSCTDYSSCFSFKPDKEHELNFKITYVDGSLITDTKSVVKAKFTEGIRRNYAFYAFGTDHQEPELPTIEKKTFVFESHLNASGVAPFKVVLPDLPDIANFTRYYSIELEFVDEKRDLYTTYPYREPKQIENPSSEEEKEWFRAEVQRPKDVWNLKIGQEYQVILNSSRPLKYFVYNIVGRGNILETKRVDLAEPQTTVNVTIKPTFLTTPYGRVYFYYVDETGEFRYTEETFSVEVELQNQIEIKAPAEVKPGADVALEIKTSPKSFVGLLAVDQSVLLLGSNNDLNKESFNWRLNGYDTSTPWQGGYSYYPGERTGVVTMTNAYFFYNRTAPDYNIQGFGGSSFAMRKTTVAHDSHVFHSGAGGPTQAVGFSAESASASAAPVVRKNFAETWIFADIESTEEEVFKWVKTIPDTITNWVVTGFSLHPQKGLGVTNDQTNIKTFQPFFVSVRLPYSVKRGEVINVPALVFNYLPKTLDVELTLDNEDQEYDFVDASNEVIGDQKRTQNIRVGANEAAGASFLIRPKVIGNILLKFKAISPLAGDAIHKPLKVVPEGITQYQNRAFFINLKDTGEFKNTFELEVPEDVVPDSERVEFGLVGDLLGPVVKNLENLLRLPSGCGEQTMSKLVPNYLVRDYLKSIKKLTPALDTRIKRNLQDGYQHMLHYRHDDGSFSSFGPTKWRQEDPVRNGSTWLTAYVLRSFSKIKDIIDLDEQILAKGYEFLLTRQAENGSFTEHGEYFYSSQRSLLTLTANSLLALLEEEKPNQAAIDKAVAYLSANTAESIELLPKSIAIYALQKAKAPEAAKQVASLKSLAKHEDDRTWWTEDLDKLRASKNCGRWWCWIWSQDVEITSYALLSLLDSDQETADSVLNTVRWLIAQRNGFGGFASSQDTVVGLTALIKFAEKSGYEAAKWEVTVSNKGKREKTEKLNTSEENDLLLQTVEFPQGTKSLEFEAKGTGAAMVQISYQYNLVEKEPKPSFKIQTTVLPESSPANLELSVCVDYVEEGESKESNMAILEVSLPSGYTADEDSFADIRNIERVRLVETKNGDSVVVIYFENLAKNEEKCIRIEAYRTHAVANQKPSSVVLYDYYDTNKKATEYYSIKSKLCDICEGDDCKSKC

Target ORF information:

RefSeq Version	NM_078879.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Thioester-containing protein 4 (Tep4), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078879.3

ORF Insert Sequence:

ATGCGTCGCG CAGACGCTTT TGCTGTCTCG CTTTGTGTGA TTTTGGCCTT GCTGCAGACG 
ATGGAACCGG TAAAAGCCGA GGGCAAGTAC ACCATTGTGG GTCCCGGAAC CATCCACTCC 
CACCGTGACT ACAATGTGGC CGTGGCTGTC CATCAGACCA AGGAGCCAGT CACCCTGAAA 
GTGGGCATCA CTGGACCCTC CTACAACAAA ACTGAGACCG TGGAACTAGC CACTGCCGGT 
GAGTTCAAGC AGATCACCTT CAAACTGCCC CCATTGGAGG CGGGTGAATA TAATCTGACG 
GCGGAGGGCG TCAAGGGACT GGAGTTCAAG AACTCCACCA AGCTGAACTG GGAGAATTTC 
AAGCCATACA TTAAAATCCA GACCGATAAG GGAAAGTACA AGCCCGGTGA TACTATCAAC 
TATCGTGTGA TCTTCCTGGA CGAGAACCTG CGGCCGGATA CCGCTAAGGA TGAAGTGGTG 
GTTTGGTTTG AGGACTCCAA AAGGAACCGC ATCAAGCAGG AGAAACACAT AAAGACCACC 
GGTGGCGTCT ACACTGGCAA ATTCGAGCTC TCCGAGTTCG CCACATTGGG CTCCTGGTCC 
CTTCATGTTC AGAATGGAGA TCAGCACCAC GACGGAGGTA TTTACTTTGG GGGTCGTAAA 
CAATTTGGAG GATTTGGTCA TCGCTGGCAT CGCTCGGATG AGCTCGTCAA CTTTGAGGTG 
GAGAAGTACG TGCTGCCCAA GTACTCCGTC AAAATGGATG CCACGCAGCA GGTTTCAGTG 
CGAGATGGCG AGTTCAATGT CGTCCTGAAG GCCAACTACA CCTATGGTAA GCCCGTGAAT 
GGTAAAGTTC TTGTCAATGT CCATCTGGAC TCGACGAGCA GTTGGGAGAA TGTGGACGGC 
AAGACAGTGC AAACGGACTA CCCCGGACAT TCTGTTGTTG GCACCGCCGA CATGGTCGGA 
GGCAAGGCCA AGTTGACCAT GGATCTCAAG GACTTCGCCA GTTATCTGCC GCACAAGACC 
TCTTCTTCAT ATGCCCAGAT TACGGCTACC GTGGAGGAAG ACTTCACCGG CGTGAAGCTC 
AATGAAACAG GCGGTGTGCA GCTGTACCCC TATCGGTATG AGATGTCCTG CACTGACTAC 
TCCAGTTGCT TCTCCTTCAA GCCCGACAAG GAACATGAAC TTAACTTCAA GATTACATAT 
GTCGACGGCT CACTAATTAC AGACACCAAG TCTGTTGTGA AGGCCAAGTT CACTGAAGGA 
ATTCGACGCA ATTATGCTTT CTATGCCTTT GGAACAGATC ATCAAGAACC GGAATTGCCA 
ACGATTGAGA AGAAAACGTT TGTGTTTGAA AGCCATTTGA ATGCGTCTGG TGTGGCGCCT 
TTCAAGGTGG TTCTTCCCGA TTTGCCGGAT ATCGCCAACT TTACTCGTTA CTATAGCATT 
GAGCTGGAGT TCGTCGATGA AAAACGAGAT CTGTACACCA CTTATCCCTA CAGGGAACCA 
AAACAGATTG AAAATCCTAG CTCTGAAGAG GAAAAGGAGT GGTTCAGGGC AGAAGTTCAG 
AGGCCCAAGG ACGTCTGGAA CCTCAAGATC GGGCAGGAGT ATCAAGTAAT CCTCAACTCC 
AGTCGTCCGC TCAAATACTT TGTGTACAAC ATTGTGGGAC GTGGAAACAT ATTGGAAACG 
AAACGTGTGG ACCTCGCGGA ACCCCAAACG ACCGTCAACG TAACCATTAA GCCCACTTTC 
CTCACTACTC CCTATGGTCG GGTGTACTTC TACTACGTGG ATGAGACAGG AGAGTTCCGA 
TATACCGAGG AAACATTCAG TGTGGAGGTC GAGCTGCAGA ACCAGATCGA AATCAAGGCT 
CCCGCGGAAG TTAAACCGGG TGCCGACGTT GCACTGGAGA TCAAGACGTC GCCAAAGTCA 
TTCGTTGGAT TGTTGGCCGT CGATCAGAGT GTCCTTCTGC TGGGCAGCAA CAACGATTTG 
AACAAGGAAT CCTTCAACTG GCGCCTAAAT GGCTATGACA CCTCAACTCC CTGGCAGGGT 
GGATACTCCT ACTACCCCGG AGAGCGAACT GGAGTCGTGA CTATGACCAA TGCCTATTTC 
TTCTATAATC GCACCGCCCC AGACTACAAT ATTCAAGGAT TTGGTGGAAG CTCTTTCGCC 
ATGAGAAAGA CTACAGTGGC ACATGATAGT CATGTATTCC ATTCCGGTGC TGGCGGACCA 
ACTCAGGCCG TGGGATTCTC CGCCGAAAGT GCGTCTGCAT CTGCAGCACC AGTAGTGCGA 
AAGAATTTCG CAGAGACCTG GATCTTTGCC GATATCGAGA GCACCGAGGA GGAGGTGTTC 
AAGTGGGTAA AGACCATACC CGATACGATC ACCAACTGGG TGGTCACCGG CTTTTCACTG 
CATCCCCAGA AGGGATTAGG TGTCACCAAT GATCAGACAA ATATCAAGAC ATTCCAGCCG 
TTCTTTGTCT CCGTTCGATT GCCATACTCC GTGAAGCGAG GTGAGGTGAT CAATGTACCG 
GCACTGGTCT TTAACTACCT GCCCAAGACA CTGGACGTGG AACTGACCCT GGACAACGAG 
GATCAAGAGT ATGACTTCGT GGATGCCTCG AATGAGGTTA TTGGTGACCA GAAGCGTACG 
CAGAACATCA GAGTGGGCGC CAACGAAGCG GCAGGAGCCT CATTTTTGAT CCGACCCAAG 
GTCATTGGAA ATATTTTGCT GAAATTCAAG GCCATCTCAC CGCTGGCCGG AGATGCTATA 
CACAAGCCGC TAAAGGTAGT GCCCGAAGGA ATTACCCAGT ACCAGAACAG GGCATTCTTT 
ATCAATCTTA AGGATACGGG TGAGTTTAAG AACACCTTCG AGCTGGAAGT GCCGGAGGAT 
GTGGTCCCCG ACTCCGAGCG TGTGGAATTC GGATTGGTGG GTGATCTCTT GGGACCAGTG 
GTCAAGAACC TTGAGAACCT GTTGCGATTG CCCAGCGGTT GCGGTGAGCA GACCATGTCC 
AAATTGGTGC CCAACTATCT GGTGAGGGAT TATCTGAAGA GCATCAAGAA ACTCACACCT 
GCATTGGACA CTCGCATCAA GAGGAATCTG CAGGACGGAT ACCAACATAT GTTGCACTAT 
CGCCACGACG ACGGCAGCTT CAGTTCCTTT GGGCCCACCA AATGGAGGCA GGAGGATCCA 
GTGCGCAATG GTTCCACCTG GCTGACGGCC TATGTGCTGC GCTCGTTTAG CAAGATCAAG 
GATATAATTG ACTTGGATGA GCAGATCCTG GCCAAGGGCT ACGAATTCCT GTTAACTCGA 
CAGGCAGAAA ATGGTAGCTT CACGGAACAC GGAGAGTATT TCTATAGCTC CCAGCGATCT 
CTATTGACAT TGACCGCAAA TTCCTTGCTC GCCCTGCTGG AGGAGGAGAA ACCGAACCAG 
GCTGCCATTG ACAAGGCGGT TGCCTATCTG AGCGCCAATA CTGCCGAGTC CATTGAGCTC 
CTGCCCAAAT CCATTGCCAT TTATGCCCTG CAAAAGGCCA AGGCGCCGGA AGCTGCCAAG 
CAGGTGGCCA GTCTTAAGTC CCTGGCCAAA CACGAGGACG ATCGCACCTG GTGGACCGAG 
GATCTGGATA AGCTGCGTGC CTCCAAGAAC TGCGGACGCT GGTGGTGTTG GATCTGGAGT 
CAGGATGTGG AGATCACATC ATATGCGCTG CTTTCCCTTC TGGATTCAGA CCAGGAGACA 
GCCGACTCTG TTCTGAACAC CGTTCGCTGG TTGATCGCCC AGCGTAATGG CTTCGGAGGA 
TTTGCCTCCA GCCAGGACAC GGTTGTCGGC CTCACCGCCC TCATTAAGTT CGCTGAGAAG 
TCGGGCTACG AGGCGGCCAA GTGGGAGGTG ACCGTTTCCA ACAAGGGAAA GCGTGAAAAG 
ACCGAGAAGC TCAACACATC GGAGGAAAAT GACCTGCTCC TCCAGACAGT CGAGTTCCCA 
CAGGGCACCA AGTCGCTGGA GTTTGAAGCC AAGGGAACTG GAGCTGCTAT GGTCCAGATA 
AGCTACCAAT ACAACTTGGT GGAGAAGGAG CCGAAGCCGA GCTTTAAGAT TCAGACCACC 
GTTTTGCCGG AATCGTCGCC AGCCAATTTG GAACTAAGCG TCTGCGTGGA CTACGTGGAG 
GAGGGCGAGT CCAAGGAATC CAACATGGCC ATTCTGGAGG TATCCCTGCC GTCTGGTTAT 
ACTGCTGATG AGGATAGCTT CGCCGACATC CGGAACATCG AACGTGTGCG GTTGGTGGAA 
ACCAAGAACG GTGACTCCGT GGTGGTCATA TACTTCGAGA ATCTGGCCAA GAACGAAGAA 
AAATGCATTC GCATAGAGGC GTACAGAACA CATGCGGTGG CCAACCAGAA GCCATCATCG 
GTGGTCCTCT ACGATTACTA TGACACGAAC AAGAAGGCCA CCGAATACTA CTCCATCAAG 
TCGAAGCTCT GCGACATTTG CGAGGGTGAC GACTGCAAGA GCAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tep4 ( NM_001273662.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260591.1 Drosophila melanogaster Thioester-containing protein 4 (Tep4), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa16894
Clone ID Related Accession (Same CDS sequence)	NM_001273662.1
Accession Version	NM_001273662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Thioester-containing protein 4, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACCGG TAAAAGCCGA GGGCAAGTAC ACCATTGTGG GTCCCGGAAC CATCCACTCC 
CACCGTGACT ACAATGTGGC CGTGGCTGTC CATCAGACCA AGGAGCCAGT CACCCTGAAA 
GTGGGCATCA CTGGACCCTC CTACAACAAA ACTGAGACCG TGGAACTAGC CACTGCCGGT 
GAGTTCAAGC AGATCACCTT CAAACTGCCC CCATTGGAGG CGGGTGAATA TAATCTGACG 
GCGGAGGGCG TCAAGGGACT GGAGTTCAAG AACTCCACCA AGCTGAACTG GGAGAATTTC 
AAGCCATACA TTAAAATCCA GACCGATAAG GGAAAGTACA AGCCCGGTGA TACTATCAAC 
TATCGTGTGA TCTTCCTGGA CGAGAACCTG CGGCCGGATA CCGCTAAGGA TGAAGTGGTG 
GTTTGGTTTG AGGACTCCAA AAGGAACCGC ATCAAGCAGG AGAAACACAT AAAGACCACC 
GGTGGCGTCT ACACTGGCAA ATTCGAGCTC TCCGAGTTCG CCACATTGGG CTCCTGGTCC 
CTTCATGTTC AGAATGGAGA TCAGCACCAC GACGGAGGTA TTTACTTTGG GGGTCGTAAA 
CAATTTGGAG GATTTGGTCA TCGCTGGCAT CGCTCGGATG AGCTCGTCAA CTTTGAGGTG 
GAGAAGTACG TGCTGCCCAA GTACTCCGTC AAAATGGATG CCACGCAGCA GGTTTCAGTG 
CGAGATGGCG AGTTCAATGT CGTCCTGAAG GCCAACTACA CCTATGGTAA GCCCGTGAAT 
GGTAAAGTTC TTGTCAATGT CCATCTGGAC TCGACGAGCA GTTGGGAGAA TGTGGACGGC 
AAGACAGTGC AAACGGACTA CCCCGGACAT TCTGTTGTTG GCACCGCCGA CATGGTCGGA 
GGCAAGGCCA AGTTGACCAT GGATCTCAAG GACTTCGCCA GTTATCTGCC GCACAAGACC 
TCTTCTTCAT ATGCCCAGAT TACGGCTACC GTGGAGGAAG ACTTCACCGG CGTGAAGCTC 
AATGAAACAG GCGGTGTGCA GCTGTACCCC TATCGGTATG AGATGTCCTG CACTGACTAC 
TCCAGTTGCT TCTCCTTCAA GCCCGACAAG GAACATGAAC TTAACTTCAA GATTACATAT 
GTCGACGGCT CACTAATTAC AGACACCAAG TCTGTTGTGA AGGCCAAGTT CACTGAAGGA 
ATTCGACGCA ATTATGCTTT CTATGCCTTT GGAACAGATC ATCAAGAACC GGAATTGCCA 
ACGATTGAGA AGAAAACGTT TGTGTTTGAA AGCCATTTGA ATGCGTCTGG TGTGGCGCCT 
TTCAAGGTGG TTCTTCCCGA TTTGCCGGAT ATCGCCAACT TTACTCGTTA CTATAGCATT 
GAGCTGGAGT TCGTCGATGA AAAACGAGAT CTGTACACCA CTTATCCCTA CAGGGAACCA 
AAACAGATTG AAAATCCTAG CTCTGAAGAG GAAAAGGAGT GGTTCAGGGC AGAAGTTCAG 
AGGCCCAAGG ACGTCTGGAA CCTCAAGATC GGGCAGGAGT ATCAAGTAAT CCTCAACTCC 
AGTCGTCCGC TCAAATACTT TGTGTACAAC ATTGTGGGAC GTGGAAACAT ATTGGAAACG 
AAACGTGTGG ACCTCGCGGA ACCCCAAACG ACCGTCAACG TAACCATTAA GCCCACTTTC 
CTCACTACTC CCTATGGTCG GGTGTACTTC TACTACGTGG ATGAGACAGG AGAGTTCCGA 
TATACCGAGG AAACATTCAG TGTGGAGGTC GAGCTGCAGA ACCAGATCGA AATCAAGGCT 
CCCGCGGAAG TTAAACCGGG TGCCGACGTT GCACTGGAGA TCAAGACGTC GCCAAAGTCA 
TTCGTTGGAT TGTTGGCCGT CGATCAGAGT GTCCTTCTGC TGGGCAGCAA CAACGATTTG 
AACAAGGAAT CCTTCAACTG GCGCCTAAAT GGCTATGACA CCTCAACTCC CTGGCAGGGT 
GGATACTCCT ACTACCCCGG AGAGCGAACT GGAGTCGTGA CTATGACCAA TGCCTATTTC 
TTCTATAATC GCACCGCCCC AGACTACAAT ATTCTTACAG AAGGATTTGG TGGAAGCTCT 
TTCGCCATGA GAAAGACTAC AGTGGCACAT GATAGTCATG TATTCCATTC CGGTGCTGGC 
GGACCAACTC AGGCCGTGGG ATTCTCCGCC GAAAGTGCGT CTGCATCTGC AGCACCAGTA 
GTGCGAAAGA ATTTCGCAGA GACCTGGATC TTTGCCGATA TCGAGAGCAC CGAGGAGGAG 
GTGTTCAAGT GGGTAAAGAC CATACCCGAT ACGATCACCA ACTGGGTGGT CACCGGCTTT 
TCACTGCATC CCCAGAAGGG ATTAGGTGTC ACCAATGATC AGACAAATAT CAAGACATTC 
CAGCCGTTCT TTGTCTCCGT TCGATTGCCA TACTCCGTGA AGCGAGGTGA GGTGATCAAT 
GTACCGGCAC TGGTCTTTAA CTACCTGCCC AAGACACTGG ACGTGGAACT GACCCTGGAC 
AACGAGGATC AAGAGTATGA CTTCGTGGAT GCCTCGAATG AGGTTATTGG TGACCAGAAG 
CGTACGCAGA ACATCAGAGT GGGCGCCAAC GAAGCGGCAG GAGCCTCATT TTTGATCCGA 
CCCAAGGTCA TTGGAAATAT TTTGCTGAAA TTCAAGGCCA TCTCACCGCT GGCCGGAGAT 
GCTATACACA AGCCGCTAAA GGTAGTGCCC GAAGGAATTA CCCAGTACCA GAACAGGGCA 
TTCTTTATCA ATCTTAAGGA TACGGGTGAG TTTAAGAACA CCTTCGAGCT GGAAGTGCCG 
GAGGATGTGG TCCCCGACTC CGAGCGTGTG GAATTCGGAT TGGTGGGTGA TCTCTTGGGA 
CCAGTGGTCA AGAACCTTGA GAACCTGTTG CGATTGCCCA GCGGTTGCGG TGAGCAGACC 
ATGTCCAAAT TGGTGCCCAA CTATCTGGTG AGGGATTATC TGAAGAGCAT CAAGAAACTC 
ACACCTGCAT TGGACACTCG CATCAAGAGG AATCTGCAGG ACGGATACCA ACATATGTTG 
CACTATCGCC ACGACGACGG CAGCTTCAGT TCCTTTGGGC CCACCAAATG GAGGCAGGAG 
GATCCAGTGC GCAATGGTTC CACCTGGCTG ACGGCCTATG TGCTGCGCTC GTTTAGCAAG 
ATCAAGGATA TAATTGACTT GGATGAGCAG ATCCTGGCCA AGGGCTACGA ATTCCTGTTA 
ACTCGACAGG CAGAAAATGG TAGCTTCACG GAACACGGAG AGTATTTCTA TAGCTCCCAG 
CGATCTCTAT TGACATTGAC CGCAAATTCC TTGCTCGCCC TGCTGGAGGA GGAGAAACCG 
AACCAGGCTG CCATTGACAA GGCGGTTGCC TATCTGAGCG CCAATACTGC CGAGTCCATT 
GAGCTCCTGC CCAAATCCAT TGCCATTTAT GCCCTGCAAA AGGCCAAGGC GCCGGAAGCT 
GCCAAGCAGG TGGCCAGTCT TAAGTCCCTG GCCAAACACG AGGACGATCG CACCTGGTGG 
ACCGAGGATC TGGATAAGCT GCGTGCCTCC AAGAACTGCG GACGCTGGTG GTGTTGGATC 
TGGAGTCAGG ATGTGGAGAT CACATCATAT GCGCTGCTTT CCCTTCTGGA TTCAGACCAG 
GAGACAGCCG ACTCTGTTCT GAACACCGTT CGCTGGTTGA TCGCCCAGCG TAATGGCTTC 
GGAGGATTTG CCTCCAGCCA GGACACGGTT GTCGGCCTCA CCGCCCTCAT TAAGTTCGCT 
GAGAAGTCGG GCTACGAGGC GGCCAAGTGG GAGGTGACCG TTTCCAACAA GGGAAAGCGT 
GAAAAGACCG AGAAGCTCAA CACATCGGAG GAAAATGACC TGCTCCTCCA GACAGTCGAG 
TTCCCACAGG GCACCAAGTC GCTGGAGTTT GAAGCCAAGG GAACTGGAGC TGCTATGGTC 
CAGATAAGCT ACCAATACAA CTTGGTGGAG AAGGAGCCGA AGCCGAGCTT TAAGATTCAG 
ACCACCGTTT TGCCGGAATC GTCGCCAGCC AATTTGGAAC TAAGCGTCTG CGTGGACTAC 
GTGGAGGAGG GCGAGTCCAA GGAATCCAAC ATGGCCATTC TGGAGGTATC CCTGCCGTCT 
GGTTATACTG CTGATGAGGA TAGCTTCGCC GACATCCGGA ACATCGAACG TGTGCGGTTG 
GTGGAAACCA AGAACGGTGA CTCCGTGGTG GTCATATACT TCGAGAATCT GGCCAAGAAC 
GAAGAAAAAT GCATTCGCAT AGAGGCGTAC AGAACACATG CGGTGGCCAA CCAGAAGCCA 
TCATCGGTGG TCCTCTACGA TTACTATGAC ACGAACAAGA AGGCCACCGA ATACTACTCC 
ATCAAGTCGA AGCTCTGCGA CATTTGCGAG GGTGACGACT GCAAGAGCAA GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260591.1
CDS	237..4673
Translation	MEPVKAEGKYTIVGPGTIHSHRDYNVAVAVHQTKEPVTLKVGITGPSYNKTETVELATAGEFKQITFKLPPLEAGEYNLTAEGVKGLEFKNSTKLNWENFKPYIKIQTDKGKYKPGDTINYRVIFLDENLRPDTAKDEVVVWFEDSKRNRIKQEKHIKTTGGVYTGKFELSEFATLGSWSLHVQNGDQHHDGGIYFGGRKQFGGFGHRWHRSDELVNFEVEKYVLPKYSVKMDATQQVSVRDGEFNVVLKANYTYGKPVNGKVLVNVHLDSTSSWENVDGKTVQTDYPGHSVVGTADMVGGKAKLTMDLKDFASYLPHKTSSSYAQITATVEEDFTGVKLNETGGVQLYPYRYEMSCTDYSSCFSFKPDKEHELNFKITYVDGSLITDTKSVVKAKFTEGIRRNYAFYAFGTDHQEPELPTIEKKTFVFESHLNASGVAPFKVVLPDLPDIANFTRYYSIELEFVDEKRDLYTTYPYREPKQIENPSSEEEKEWFRAEVQRPKDVWNLKIGQEYQVILNSSRPLKYFVYNIVGRGNILETKRVDLAEPQTTVNVTIKPTFLTTPYGRVYFYYVDETGEFRYTEETFSVEVELQNQIEIKAPAEVKPGADVALEIKTSPKSFVGLLAVDQSVLLLGSNNDLNKESFNWRLNGYDTSTPWQGGYSYYPGERTGVVTMTNAYFFYNRTAPDYNILTEGFGGSSFAMRKTTVAHDSHVFHSGAGGPTQAVGFSAESASASAAPVVRKNFAETWIFADIESTEEEVFKWVKTIPDTITNWVVTGFSLHPQKGLGVTNDQTNIKTFQPFFVSVRLPYSVKRGEVINVPALVFNYLPKTLDVELTLDNEDQEYDFVDASNEVIGDQKRTQNIRVGANEAAGASFLIRPKVIGNILLKFKAISPLAGDAIHKPLKVVPEGITQYQNRAFFINLKDTGEFKNTFELEVPEDVVPDSERVEFGLVGDLLGPVVKNLENLLRLPSGCGEQTMSKLVPNYLVRDYLKSIKKLTPALDTRIKRNLQDGYQHMLHYRHDDGSFSSFGPTKWRQEDPVRNGSTWLTAYVLRSFSKIKDIIDLDEQILAKGYEFLLTRQAENGSFTEHGEYFYSSQRSLLTLTANSLLALLEEEKPNQAAIDKAVAYLSANTAESIELLPKSIAIYALQKAKAPEAAKQVASLKSLAKHEDDRTWWTEDLDKLRASKNCGRWWCWIWSQDVEITSYALLSLLDSDQETADSVLNTVRWLIAQRNGFGGFASSQDTVVGLTALIKFAEKSGYEAAKWEVTVSNKGKREKTEKLNTSEENDLLLQTVEFPQGTKSLEFEAKGTGAAMVQISYQYNLVEKEPKPSFKIQTTVLPESSPANLELSVCVDYVEEGESKESNMAILEVSLPSGYTADEDSFADIRNIERVRLVETKNGDSVVVIYFENLAKNEEKCIRIEAYRTHAVANQKPSSVVLYDYYDTNKKATEYYSIKSKLCDICEGDDCKSKC

Target ORF information:

RefSeq Version	NM_001273662.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Thioester-containing protein 4 (Tep4), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273662.1

ORF Insert Sequence:

ATGGAACCGG TAAAAGCCGA GGGCAAGTAC ACCATTGTGG GTCCCGGAAC CATCCACTCC 
CACCGTGACT ACAATGTGGC CGTGGCTGTC CATCAGACCA AGGAGCCAGT CACCCTGAAA 
GTGGGCATCA CTGGACCCTC CTACAACAAA ACTGAGACCG TGGAACTAGC CACTGCCGGT 
GAGTTCAAGC AGATCACCTT CAAACTGCCC CCATTGGAGG CGGGTGAATA TAATCTGACG 
GCGGAGGGCG TCAAGGGACT GGAGTTCAAG AACTCCACCA AGCTGAACTG GGAGAATTTC 
AAGCCATACA TTAAAATCCA GACCGATAAG GGAAAGTACA AGCCCGGTGA TACTATCAAC 
TATCGTGTGA TCTTCCTGGA CGAGAACCTG CGGCCGGATA CCGCTAAGGA TGAAGTGGTG 
GTTTGGTTTG AGGACTCCAA AAGGAACCGC ATCAAGCAGG AGAAACACAT AAAGACCACC 
GGTGGCGTCT ACACTGGCAA ATTCGAGCTC TCCGAGTTCG CCACATTGGG CTCCTGGTCC 
CTTCATGTTC AGAATGGAGA TCAGCACCAC GACGGAGGTA TTTACTTTGG GGGTCGTAAA 
CAATTTGGAG GATTTGGTCA TCGCTGGCAT CGCTCGGATG AGCTCGTCAA CTTTGAGGTG 
GAGAAGTACG TGCTGCCCAA GTACTCCGTC AAAATGGATG CCACGCAGCA GGTTTCAGTG 
CGAGATGGCG AGTTCAATGT CGTCCTGAAG GCCAACTACA CCTATGGTAA GCCCGTGAAT 
GGTAAAGTTC TTGTCAATGT CCATCTGGAC TCGACGAGCA GTTGGGAGAA TGTGGACGGC 
AAGACAGTGC AAACGGACTA CCCCGGACAT TCTGTTGTTG GCACCGCCGA CATGGTCGGA 
GGCAAGGCCA AGTTGACCAT GGATCTCAAG GACTTCGCCA GTTATCTGCC GCACAAGACC 
TCTTCTTCAT ATGCCCAGAT TACGGCTACC GTGGAGGAAG ACTTCACCGG CGTGAAGCTC 
AATGAAACAG GCGGTGTGCA GCTGTACCCC TATCGGTATG AGATGTCCTG CACTGACTAC 
TCCAGTTGCT TCTCCTTCAA GCCCGACAAG GAACATGAAC TTAACTTCAA GATTACATAT 
GTCGACGGCT CACTAATTAC AGACACCAAG TCTGTTGTGA AGGCCAAGTT CACTGAAGGA 
ATTCGACGCA ATTATGCTTT CTATGCCTTT GGAACAGATC ATCAAGAACC GGAATTGCCA 
ACGATTGAGA AGAAAACGTT TGTGTTTGAA AGCCATTTGA ATGCGTCTGG TGTGGCGCCT 
TTCAAGGTGG TTCTTCCCGA TTTGCCGGAT ATCGCCAACT TTACTCGTTA CTATAGCATT 
GAGCTGGAGT TCGTCGATGA AAAACGAGAT CTGTACACCA CTTATCCCTA CAGGGAACCA 
AAACAGATTG AAAATCCTAG CTCTGAAGAG GAAAAGGAGT GGTTCAGGGC AGAAGTTCAG 
AGGCCCAAGG ACGTCTGGAA CCTCAAGATC GGGCAGGAGT ATCAAGTAAT CCTCAACTCC 
AGTCGTCCGC TCAAATACTT TGTGTACAAC ATTGTGGGAC GTGGAAACAT ATTGGAAACG 
AAACGTGTGG ACCTCGCGGA ACCCCAAACG ACCGTCAACG TAACCATTAA GCCCACTTTC 
CTCACTACTC CCTATGGTCG GGTGTACTTC TACTACGTGG ATGAGACAGG AGAGTTCCGA 
TATACCGAGG AAACATTCAG TGTGGAGGTC GAGCTGCAGA ACCAGATCGA AATCAAGGCT 
CCCGCGGAAG TTAAACCGGG TGCCGACGTT GCACTGGAGA TCAAGACGTC GCCAAAGTCA 
TTCGTTGGAT TGTTGGCCGT CGATCAGAGT GTCCTTCTGC TGGGCAGCAA CAACGATTTG 
AACAAGGAAT CCTTCAACTG GCGCCTAAAT GGCTATGACA CCTCAACTCC CTGGCAGGGT 
GGATACTCCT ACTACCCCGG AGAGCGAACT GGAGTCGTGA CTATGACCAA TGCCTATTTC 
TTCTATAATC GCACCGCCCC AGACTACAAT ATTCTTACAG AAGGATTTGG TGGAAGCTCT 
TTCGCCATGA GAAAGACTAC AGTGGCACAT GATAGTCATG TATTCCATTC CGGTGCTGGC 
GGACCAACTC AGGCCGTGGG ATTCTCCGCC GAAAGTGCGT CTGCATCTGC AGCACCAGTA 
GTGCGAAAGA ATTTCGCAGA GACCTGGATC TTTGCCGATA TCGAGAGCAC CGAGGAGGAG 
GTGTTCAAGT GGGTAAAGAC CATACCCGAT ACGATCACCA ACTGGGTGGT CACCGGCTTT 
TCACTGCATC CCCAGAAGGG ATTAGGTGTC ACCAATGATC AGACAAATAT CAAGACATTC 
CAGCCGTTCT TTGTCTCCGT TCGATTGCCA TACTCCGTGA AGCGAGGTGA GGTGATCAAT 
GTACCGGCAC TGGTCTTTAA CTACCTGCCC AAGACACTGG ACGTGGAACT GACCCTGGAC 
AACGAGGATC AAGAGTATGA CTTCGTGGAT GCCTCGAATG AGGTTATTGG TGACCAGAAG 
CGTACGCAGA ACATCAGAGT GGGCGCCAAC GAAGCGGCAG GAGCCTCATT TTTGATCCGA 
CCCAAGGTCA TTGGAAATAT TTTGCTGAAA TTCAAGGCCA TCTCACCGCT GGCCGGAGAT 
GCTATACACA AGCCGCTAAA GGTAGTGCCC GAAGGAATTA CCCAGTACCA GAACAGGGCA 
TTCTTTATCA ATCTTAAGGA TACGGGTGAG TTTAAGAACA CCTTCGAGCT GGAAGTGCCG 
GAGGATGTGG TCCCCGACTC CGAGCGTGTG GAATTCGGAT TGGTGGGTGA TCTCTTGGGA 
CCAGTGGTCA AGAACCTTGA GAACCTGTTG CGATTGCCCA GCGGTTGCGG TGAGCAGACC 
ATGTCCAAAT TGGTGCCCAA CTATCTGGTG AGGGATTATC TGAAGAGCAT CAAGAAACTC 
ACACCTGCAT TGGACACTCG CATCAAGAGG AATCTGCAGG ACGGATACCA ACATATGTTG 
CACTATCGCC ACGACGACGG CAGCTTCAGT TCCTTTGGGC CCACCAAATG GAGGCAGGAG 
GATCCAGTGC GCAATGGTTC CACCTGGCTG ACGGCCTATG TGCTGCGCTC GTTTAGCAAG 
ATCAAGGATA TAATTGACTT GGATGAGCAG ATCCTGGCCA AGGGCTACGA ATTCCTGTTA 
ACTCGACAGG CAGAAAATGG TAGCTTCACG GAACACGGAG AGTATTTCTA TAGCTCCCAG 
CGATCTCTAT TGACATTGAC CGCAAATTCC TTGCTCGCCC TGCTGGAGGA GGAGAAACCG 
AACCAGGCTG CCATTGACAA GGCGGTTGCC TATCTGAGCG CCAATACTGC CGAGTCCATT 
GAGCTCCTGC CCAAATCCAT TGCCATTTAT GCCCTGCAAA AGGCCAAGGC GCCGGAAGCT 
GCCAAGCAGG TGGCCAGTCT TAAGTCCCTG GCCAAACACG AGGACGATCG CACCTGGTGG 
ACCGAGGATC TGGATAAGCT GCGTGCCTCC AAGAACTGCG GACGCTGGTG GTGTTGGATC 
TGGAGTCAGG ATGTGGAGAT CACATCATAT GCGCTGCTTT CCCTTCTGGA TTCAGACCAG 
GAGACAGCCG ACTCTGTTCT GAACACCGTT CGCTGGTTGA TCGCCCAGCG TAATGGCTTC 
GGAGGATTTG CCTCCAGCCA GGACACGGTT GTCGGCCTCA CCGCCCTCAT TAAGTTCGCT 
GAGAAGTCGG GCTACGAGGC GGCCAAGTGG GAGGTGACCG TTTCCAACAA GGGAAAGCGT 
GAAAAGACCG AGAAGCTCAA CACATCGGAG GAAAATGACC TGCTCCTCCA GACAGTCGAG 
TTCCCACAGG GCACCAAGTC GCTGGAGTTT GAAGCCAAGG GAACTGGAGC TGCTATGGTC 
CAGATAAGCT ACCAATACAA CTTGGTGGAG AAGGAGCCGA AGCCGAGCTT TAAGATTCAG 
ACCACCGTTT TGCCGGAATC GTCGCCAGCC AATTTGGAAC TAAGCGTCTG CGTGGACTAC 
GTGGAGGAGG GCGAGTCCAA GGAATCCAAC ATGGCCATTC TGGAGGTATC CCTGCCGTCT 
GGTTATACTG CTGATGAGGA TAGCTTCGCC GACATCCGGA ACATCGAACG TGTGCGGTTG 
GTGGAAACCA AGAACGGTGA CTCCGTGGTG GTCATATACT TCGAGAATCT GGCCAAGAAC 
GAAGAAAAAT GCATTCGCAT AGAGGCGTAC AGAACACATG CGGTGGCCAA CCAGAAGCCA 
TCATCGGTGG TCCTCTACGA TTACTATGAC ACGAACAAGA AGGCCACCGA ATACTACTCC 
ATCAAGTCGA AGCTCTGCGA CATTTGCGAG GGTGACGACT GCAAGAGCAA GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tep4 ( NM_001273660.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260589.1 Drosophila melanogaster Thioester-containing protein 4 (Tep4), transcript variant B, mRNA.

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CloneID	OFa16895
Clone ID Related Accession (Same CDS sequence)	NM_001273660.1
Accession Version	NM_001273660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Thioester-containing protein 4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACCGG TAAAAGCCGA GGGCAAGTAC ACCATTGTGG GTCCCGGAAC CATCCACTCC 
CACCGTGACT ACAATGTGGC CGTGGCTGTC CATCAGACCA AGGAGCCAGT CACCCTGAAA 
GTGGGCATCA CTGGACCCTC CTACAACAAA ACTGAGACCG TGGAACTAGC CACTGCCGGT 
GAGTTCAAGC AGATCACCTT CAAACTGCCC CCATTGGAGG CGGGTGAATA TAATCTGACG 
GCGGAGGGCG TCAAGGGACT GGAGTTCAAG AACTCCACCA AGCTGAACTG GGAGAATTTC 
AAGCCATACA TTAAAATCCA GACCGATAAG GGAAAGTACA AGCCCGGTGA TACTATCAAC 
TATCGTGTGA TCTTCCTGGA CGAGAACCTG CGGCCGGATA CCGCTAAGGA TGAAGTGGTG 
GTTTGGTTTG AGGACTCCAA AAGGAACCGC ATCAAGCAGG AGAAACACAT AAAGACCACC 
GGTGGCGTCT ACACTGGCAA ATTCGAGCTC TCCGAGTTCG CCACATTGGG CTCCTGGTCC 
CTTCATGTTC AGAATGGAGA TCAGCACCAC GACGGAGGTA TTTACTTTGG GGGTCGTAAA 
CAATTTGGAG GATTTGGTCA TCGCTGGCAT CGCTCGGATG AGCTCGTCAA CTTTGAGGTG 
GAGAAGTACG TGCTGCCCAA GTACTCCGTC AAAATGGATG CCACGCAGCA GGTTTCAGTG 
CGAGATGGCG AGTTCAATGT CGTCCTGAAG GCCAACTACA CCTATGGTAA GCCCGTGAAT 
GGTAAAGTTC TTGTCAATGT CCATCTGGAC TCGACGAGCA GTTGGGAGAA TGTGGACGGC 
AAGACAGTGC AAACGGACTA CCCCGGACAT TCTGTTGTTG GCACCGCCGA CATGGTCGGA 
GGCAAGGCCA AGTTGACCAT GGATCTCAAG GACTTCGCCA GTTATCTGCC GCACAAGACC 
TCTTCTTCAT ATGCCCAGAT TACGGCTACC GTGGAGGAAG ACTTCACCGG CGTGAAGCTC 
AATGAAACAG GCGGTGTGCA GCTGTACCCC TATCGGTATG AGATGTCCTG CACTGACTAC 
TCCAGTTGCT TCTCCTTCAA GCCCGACAAG GAACATGAAC TTAACTTCAA GATTACATAT 
GTCGACGGCT CACTAATTAC AGACACCAAG TCTGTTGTGA AGGCCAAGTT CACTGAAGGA 
ATTCGACGCA ATTATGCTTT CTATGCCTTT GGAACAGATC ATCAAGAACC GGAATTGCCA 
ACGATTGAGA AGAAAACGTT TGTGTTTGAA AGCCATTTGA ATGCGTCTGG TGTGGCGCCT 
TTCAAGGTGG TTCTTCCCGA TTTGCCGGAT ATCGCCAACT TTACTCGTTA CTATAGCATT 
GAGCTGGAGT TCGTCGATGA AAAACGAGAT CTGTACACCA CTTATCCCTA CAGGGAACCA 
AAACAGATTG AAAATCCTAG CTCTGAAGAG GAAAAGGAGT GGTTCAGGGC AGAAGTTCAG 
AGGCCCAAGG ACGTCTGGAA CCTCAAGATC GGGCAGGAGT ATCAAGTAAT CCTCAACTCC 
AGTCGTCCGC TCAAATACTT TGTGTACAAC ATTGTGGGAC GTGGAAACAT ATTGGAAACG 
AAACGTGTGG ACCTCGCGGA ACCCCAAACG ACCGTCAACG TAACCATTAA GCCCACTTTC 
CTCACTACTC CCTATGGTCG GGTGTACTTC TACTACGTGG ATGAGACAGG AGAGTTCCGA 
TATACCGAGG AAACATTCAG TGTGGAGGTC GAGCTGCAGA ACCAGATCGA AATCAAGGCT 
CCCGCGGAAG TTAAACCGGG TGCCGACGTT GCACTGGAGA TCAAGACGTC GCCAAAGTCA 
TTCGTTGGAT TGTTGGCCGT CGATCAGAGT GTCCTTCTGC TGGGCAGCAA CAACGATTTG 
AACAAGGAAT CCTTCAACTG GCGCCTAAAT GGCTATGACA CCTCAACTCC CTGGCAGGGT 
GGATACTCCT ACTACCCCGG AGAGCGAACT GGAGTCGTGA CTATGACCAA TGCCTATTTC 
TTCTATAATC GCACCGCCCC AGACTACAAT ATTCAAGGAT TTGGTGGAAG CTCTTTCGCC 
ATGAGAAAGA CTACAGTGGC ACATGATAGT CATGTATTCC ATTCCGGTGC TGGCGGACCA 
ACTCAGGCCG TGGGATTCTC CGCCGAAAGT GCGTCTGCAT CTGCAGCACC AGTAGTGCGA 
AAGAATTTCG CAGAGACCTG GATCTTTGCC GATATCGAGA GCACCGAGGA GGAGGTGTTC 
AAGTGGGTAA AGACCATACC CGATACGATC ACCAACTGGG TGGTCACCGG CTTTTCACTG 
CATCCCCAGA AGGGATTAGG TGTCACCAAT GATCAGACAA ATATCAAGAC ATTCCAGCCG 
TTCTTTGTCT CCGTTCGATT GCCATACTCC GTGAAGCGAG GTGAGGTGAT CAATGTACCG 
GCACTGGTCT TTAACTACCT GCCCAAGACA CTGGACGTGG AACTGACCCT GGACAACGAG 
GATCAAGAGT ATGACTTCGT GGATGCCTCG AATGAGGTTA TTGGTGACCA GAAGCGTACG 
CAGAACATCA GAGTGGGCGC CAACGAAGCG GCAGGAGCCT CATTTTTGAT CCGACCCAAG 
GTCATTGGAA ATATTTTGCT GAAATTCAAG GCCATCTCAC CGCTGGCCGG AGATGCTATA 
CACAAGCCGC TAAAGGTAGT GCCCGAAGGA ATTACCCAGT ACCAGAACAG GGCATTCTTT 
ATCAATCTTA AGGATACGGG TGAGTTTAAG AACACCTTCG AGCTGGAAGT GCCGGAGGAT 
GTGGTCCCCG ACTCCGAGCG TGTGGAATTC GGATTGGTGG GTGATCTCTT GGGACCAGTG 
GTCAAGAACC TTGAGAACCT GTTGCGATTG CCCAGCGGTT GCGGTGAGCA GACCATGTCC 
AAATTGGTGC CCAACTATCT GGTGAGGGAT TATCTGAAGA GCATCAAGAA ACTCACACCT 
GCATTGGACA CTCGCATCAA GAGGAATCTG CAGGACGGAT ACCAACATAT GTTGCACTAT 
CGCCACGACG ACGGCAGCTT CAGTTCCTTT GGGCCCACCA AATGGAGGCA GGAGGATCCA 
GTGCGCAATG GTTCCACCTG GCTGACGGCC TATGTGCTGC GCTCGTTTAG CAAGATCAAG 
GATATAATTG ACTTGGATGA GCAGATCCTG GCCAAGGGCT ACGAATTCCT GTTAACTCGA 
CAGGCAGAAA ATGGTAGCTT CACGGAACAC GGAGAGTATT TCTATAGCTC CCAGCGATCT 
CTATTGACAT TGACCGCAAA TTCCTTGCTC GCCCTGCTGG AGGAGGAGAA ACCGAACCAG 
GCTGCCATTG ACAAGGCGGT TGCCTATCTG AGCGCCAATA CTGCCGAGTC CATTGAGCTC 
CTGCCCAAAT CCATTGCCAT TTATGCCCTG CAAAAGGCCA AGGCGCCGGA AGCTGCCAAG 
CAGGTGGCCA GTCTTAAGTC CCTGGCCAAA CACGAGGACG ATCGCACCTG GTGGACCGAG 
GATCTGGATA AGCTGCGTGC CTCCAAGAAC TGCGGACGCT GGTGGTGTTG GATCTGGAGT 
CAGGATGTGG AGATCACATC ATATGCGCTG CTTTCCCTTC TGGATTCAGA CCAGGAGACA 
GCCGACTCTG TTCTGAACAC CGTTCGCTGG TTGATCGCCC AGCGTAATGG CTTCGGAGGA 
TTTGCCTCCA GCCAGGACAC GGTTGTCGGC CTCACCGCCC TCATTAAGTT CGCTGAGAAG 
TCGGGCTACG AGGCGGCCAA GTGGGAGGTG ACCGTTTCCA ACAAGGGAAA GCGTGAAAAG 
ACCGAGAAGC TCAACACATC GGAGGAAAAT GACCTGCTCC TCCAGACAGT CGAGTTCCCA 
CAGGGCACCA AGTCGCTGGA GTTTGAAGCC AAGGGAACTG GAGCTGCTAT GGTCCAGATA 
AGCTACCAAT ACAACTTGGT GGAGAAGGAG CCGAAGCCGA GCTTTAAGAT TCAGACCACC 
GTTTTGCCGG AATCGTCGCC AGCCAATTTG GAACTAAGCG TCTGCGTGGA CTACGTGGAG 
GAGGGCGAGT CCAAGGAATC CAACATGGCC ATTCTGGAGG TATCCCTGCC GTCTGGTTAT 
ACTGCTGATG AGGATAGCTT CGCCGACATC CGGAACATCG AACGTGTGCG GTTGGTGGAA 
ACCAAGAACG GTGACTCCGT GGTGGTCATA TACTTCGAGA ATCTGGCCAA GAACGAAGAA 
AAATGCATTC GCATAGAGGC GTACAGAACA CATGCGGTGG CCAACCAGAA GCCATCATCG 
GTGGTCCTCT ACGATTACTA TGACACGAAC AAGAAGGCCA CCGAATACTA CTCCATCAAG 
TCGAAGCTCT GCGACATTTG CGAGGGTGAC GACTGCAAGA GCAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260589.1
CDS	237..4667
Translation	MEPVKAEGKYTIVGPGTIHSHRDYNVAVAVHQTKEPVTLKVGITGPSYNKTETVELATAGEFKQITFKLPPLEAGEYNLTAEGVKGLEFKNSTKLNWENFKPYIKIQTDKGKYKPGDTINYRVIFLDENLRPDTAKDEVVVWFEDSKRNRIKQEKHIKTTGGVYTGKFELSEFATLGSWSLHVQNGDQHHDGGIYFGGRKQFGGFGHRWHRSDELVNFEVEKYVLPKYSVKMDATQQVSVRDGEFNVVLKANYTYGKPVNGKVLVNVHLDSTSSWENVDGKTVQTDYPGHSVVGTADMVGGKAKLTMDLKDFASYLPHKTSSSYAQITATVEEDFTGVKLNETGGVQLYPYRYEMSCTDYSSCFSFKPDKEHELNFKITYVDGSLITDTKSVVKAKFTEGIRRNYAFYAFGTDHQEPELPTIEKKTFVFESHLNASGVAPFKVVLPDLPDIANFTRYYSIELEFVDEKRDLYTTYPYREPKQIENPSSEEEKEWFRAEVQRPKDVWNLKIGQEYQVILNSSRPLKYFVYNIVGRGNILETKRVDLAEPQTTVNVTIKPTFLTTPYGRVYFYYVDETGEFRYTEETFSVEVELQNQIEIKAPAEVKPGADVALEIKTSPKSFVGLLAVDQSVLLLGSNNDLNKESFNWRLNGYDTSTPWQGGYSYYPGERTGVVTMTNAYFFYNRTAPDYNIQGFGGSSFAMRKTTVAHDSHVFHSGAGGPTQAVGFSAESASASAAPVVRKNFAETWIFADIESTEEEVFKWVKTIPDTITNWVVTGFSLHPQKGLGVTNDQTNIKTFQPFFVSVRLPYSVKRGEVINVPALVFNYLPKTLDVELTLDNEDQEYDFVDASNEVIGDQKRTQNIRVGANEAAGASFLIRPKVIGNILLKFKAISPLAGDAIHKPLKVVPEGITQYQNRAFFINLKDTGEFKNTFELEVPEDVVPDSERVEFGLVGDLLGPVVKNLENLLRLPSGCGEQTMSKLVPNYLVRDYLKSIKKLTPALDTRIKRNLQDGYQHMLHYRHDDGSFSSFGPTKWRQEDPVRNGSTWLTAYVLRSFSKIKDIIDLDEQILAKGYEFLLTRQAENGSFTEHGEYFYSSQRSLLTLTANSLLALLEEEKPNQAAIDKAVAYLSANTAESIELLPKSIAIYALQKAKAPEAAKQVASLKSLAKHEDDRTWWTEDLDKLRASKNCGRWWCWIWSQDVEITSYALLSLLDSDQETADSVLNTVRWLIAQRNGFGGFASSQDTVVGLTALIKFAEKSGYEAAKWEVTVSNKGKREKTEKLNTSEENDLLLQTVEFPQGTKSLEFEAKGTGAAMVQISYQYNLVEKEPKPSFKIQTTVLPESSPANLELSVCVDYVEEGESKESNMAILEVSLPSGYTADEDSFADIRNIERVRLVETKNGDSVVVIYFENLAKNEEKCIRIEAYRTHAVANQKPSSVVLYDYYDTNKKATEYYSIKSKLCDICEGDDCKSKC

Target ORF information:

RefSeq Version	NM_001273660.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Thioester-containing protein 4 (Tep4), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273660.1

ORF Insert Sequence:

ATGGAACCGG TAAAAGCCGA GGGCAAGTAC ACCATTGTGG GTCCCGGAAC CATCCACTCC 
CACCGTGACT ACAATGTGGC CGTGGCTGTC CATCAGACCA AGGAGCCAGT CACCCTGAAA 
GTGGGCATCA CTGGACCCTC CTACAACAAA ACTGAGACCG TGGAACTAGC CACTGCCGGT 
GAGTTCAAGC AGATCACCTT CAAACTGCCC CCATTGGAGG CGGGTGAATA TAATCTGACG 
GCGGAGGGCG TCAAGGGACT GGAGTTCAAG AACTCCACCA AGCTGAACTG GGAGAATTTC 
AAGCCATACA TTAAAATCCA GACCGATAAG GGAAAGTACA AGCCCGGTGA TACTATCAAC 
TATCGTGTGA TCTTCCTGGA CGAGAACCTG CGGCCGGATA CCGCTAAGGA TGAAGTGGTG 
GTTTGGTTTG AGGACTCCAA AAGGAACCGC ATCAAGCAGG AGAAACACAT AAAGACCACC 
GGTGGCGTCT ACACTGGCAA ATTCGAGCTC TCCGAGTTCG CCACATTGGG CTCCTGGTCC 
CTTCATGTTC AGAATGGAGA TCAGCACCAC GACGGAGGTA TTTACTTTGG GGGTCGTAAA 
CAATTTGGAG GATTTGGTCA TCGCTGGCAT CGCTCGGATG AGCTCGTCAA CTTTGAGGTG 
GAGAAGTACG TGCTGCCCAA GTACTCCGTC AAAATGGATG CCACGCAGCA GGTTTCAGTG 
CGAGATGGCG AGTTCAATGT CGTCCTGAAG GCCAACTACA CCTATGGTAA GCCCGTGAAT 
GGTAAAGTTC TTGTCAATGT CCATCTGGAC TCGACGAGCA GTTGGGAGAA TGTGGACGGC 
AAGACAGTGC AAACGGACTA CCCCGGACAT TCTGTTGTTG GCACCGCCGA CATGGTCGGA 
GGCAAGGCCA AGTTGACCAT GGATCTCAAG GACTTCGCCA GTTATCTGCC GCACAAGACC 
TCTTCTTCAT ATGCCCAGAT TACGGCTACC GTGGAGGAAG ACTTCACCGG CGTGAAGCTC 
AATGAAACAG GCGGTGTGCA GCTGTACCCC TATCGGTATG AGATGTCCTG CACTGACTAC 
TCCAGTTGCT TCTCCTTCAA GCCCGACAAG GAACATGAAC TTAACTTCAA GATTACATAT 
GTCGACGGCT CACTAATTAC AGACACCAAG TCTGTTGTGA AGGCCAAGTT CACTGAAGGA 
ATTCGACGCA ATTATGCTTT CTATGCCTTT GGAACAGATC ATCAAGAACC GGAATTGCCA 
ACGATTGAGA AGAAAACGTT TGTGTTTGAA AGCCATTTGA ATGCGTCTGG TGTGGCGCCT 
TTCAAGGTGG TTCTTCCCGA TTTGCCGGAT ATCGCCAACT TTACTCGTTA CTATAGCATT 
GAGCTGGAGT TCGTCGATGA AAAACGAGAT CTGTACACCA CTTATCCCTA CAGGGAACCA 
AAACAGATTG AAAATCCTAG CTCTGAAGAG GAAAAGGAGT GGTTCAGGGC AGAAGTTCAG 
AGGCCCAAGG ACGTCTGGAA CCTCAAGATC GGGCAGGAGT ATCAAGTAAT CCTCAACTCC 
AGTCGTCCGC TCAAATACTT TGTGTACAAC ATTGTGGGAC GTGGAAACAT ATTGGAAACG 
AAACGTGTGG ACCTCGCGGA ACCCCAAACG ACCGTCAACG TAACCATTAA GCCCACTTTC 
CTCACTACTC CCTATGGTCG GGTGTACTTC TACTACGTGG ATGAGACAGG AGAGTTCCGA 
TATACCGAGG AAACATTCAG TGTGGAGGTC GAGCTGCAGA ACCAGATCGA AATCAAGGCT 
CCCGCGGAAG TTAAACCGGG TGCCGACGTT GCACTGGAGA TCAAGACGTC GCCAAAGTCA 
TTCGTTGGAT TGTTGGCCGT CGATCAGAGT GTCCTTCTGC TGGGCAGCAA CAACGATTTG 
AACAAGGAAT CCTTCAACTG GCGCCTAAAT GGCTATGACA CCTCAACTCC CTGGCAGGGT 
GGATACTCCT ACTACCCCGG AGAGCGAACT GGAGTCGTGA CTATGACCAA TGCCTATTTC 
TTCTATAATC GCACCGCCCC AGACTACAAT ATTCAAGGAT TTGGTGGAAG CTCTTTCGCC 
ATGAGAAAGA CTACAGTGGC ACATGATAGT CATGTATTCC ATTCCGGTGC TGGCGGACCA 
ACTCAGGCCG TGGGATTCTC CGCCGAAAGT GCGTCTGCAT CTGCAGCACC AGTAGTGCGA 
AAGAATTTCG CAGAGACCTG GATCTTTGCC GATATCGAGA GCACCGAGGA GGAGGTGTTC 
AAGTGGGTAA AGACCATACC CGATACGATC ACCAACTGGG TGGTCACCGG CTTTTCACTG 
CATCCCCAGA AGGGATTAGG TGTCACCAAT GATCAGACAA ATATCAAGAC ATTCCAGCCG 
TTCTTTGTCT CCGTTCGATT GCCATACTCC GTGAAGCGAG GTGAGGTGAT CAATGTACCG 
GCACTGGTCT TTAACTACCT GCCCAAGACA CTGGACGTGG AACTGACCCT GGACAACGAG 
GATCAAGAGT ATGACTTCGT GGATGCCTCG AATGAGGTTA TTGGTGACCA GAAGCGTACG 
CAGAACATCA GAGTGGGCGC CAACGAAGCG GCAGGAGCCT CATTTTTGAT CCGACCCAAG 
GTCATTGGAA ATATTTTGCT GAAATTCAAG GCCATCTCAC CGCTGGCCGG AGATGCTATA 
CACAAGCCGC TAAAGGTAGT GCCCGAAGGA ATTACCCAGT ACCAGAACAG GGCATTCTTT 
ATCAATCTTA AGGATACGGG TGAGTTTAAG AACACCTTCG AGCTGGAAGT GCCGGAGGAT 
GTGGTCCCCG ACTCCGAGCG TGTGGAATTC GGATTGGTGG GTGATCTCTT GGGACCAGTG 
GTCAAGAACC TTGAGAACCT GTTGCGATTG CCCAGCGGTT GCGGTGAGCA GACCATGTCC 
AAATTGGTGC CCAACTATCT GGTGAGGGAT TATCTGAAGA GCATCAAGAA ACTCACACCT 
GCATTGGACA CTCGCATCAA GAGGAATCTG CAGGACGGAT ACCAACATAT GTTGCACTAT 
CGCCACGACG ACGGCAGCTT CAGTTCCTTT GGGCCCACCA AATGGAGGCA GGAGGATCCA 
GTGCGCAATG GTTCCACCTG GCTGACGGCC TATGTGCTGC GCTCGTTTAG CAAGATCAAG 
GATATAATTG ACTTGGATGA GCAGATCCTG GCCAAGGGCT ACGAATTCCT GTTAACTCGA 
CAGGCAGAAA ATGGTAGCTT CACGGAACAC GGAGAGTATT TCTATAGCTC CCAGCGATCT 
CTATTGACAT TGACCGCAAA TTCCTTGCTC GCCCTGCTGG AGGAGGAGAA ACCGAACCAG 
GCTGCCATTG ACAAGGCGGT TGCCTATCTG AGCGCCAATA CTGCCGAGTC CATTGAGCTC 
CTGCCCAAAT CCATTGCCAT TTATGCCCTG CAAAAGGCCA AGGCGCCGGA AGCTGCCAAG 
CAGGTGGCCA GTCTTAAGTC CCTGGCCAAA CACGAGGACG ATCGCACCTG GTGGACCGAG 
GATCTGGATA AGCTGCGTGC CTCCAAGAAC TGCGGACGCT GGTGGTGTTG GATCTGGAGT 
CAGGATGTGG AGATCACATC ATATGCGCTG CTTTCCCTTC TGGATTCAGA CCAGGAGACA 
GCCGACTCTG TTCTGAACAC CGTTCGCTGG TTGATCGCCC AGCGTAATGG CTTCGGAGGA 
TTTGCCTCCA GCCAGGACAC GGTTGTCGGC CTCACCGCCC TCATTAAGTT CGCTGAGAAG 
TCGGGCTACG AGGCGGCCAA GTGGGAGGTG ACCGTTTCCA ACAAGGGAAA GCGTGAAAAG 
ACCGAGAAGC TCAACACATC GGAGGAAAAT GACCTGCTCC TCCAGACAGT CGAGTTCCCA 
CAGGGCACCA AGTCGCTGGA GTTTGAAGCC AAGGGAACTG GAGCTGCTAT GGTCCAGATA 
AGCTACCAAT ACAACTTGGT GGAGAAGGAG CCGAAGCCGA GCTTTAAGAT TCAGACCACC 
GTTTTGCCGG AATCGTCGCC AGCCAATTTG GAACTAAGCG TCTGCGTGGA CTACGTGGAG 
GAGGGCGAGT CCAAGGAATC CAACATGGCC ATTCTGGAGG TATCCCTGCC GTCTGGTTAT 
ACTGCTGATG AGGATAGCTT CGCCGACATC CGGAACATCG AACGTGTGCG GTTGGTGGAA 
ACCAAGAACG GTGACTCCGT GGTGGTCATA TACTTCGAGA ATCTGGCCAA GAACGAAGAA 
AAATGCATTC GCATAGAGGC GTACAGAACA CATGCGGTGG CCAACCAGAA GCCATCATCG 
GTGGTCCTCT ACGATTACTA TGACACGAAC AAGAAGGCCA CCGAATACTA CTCCATCAAG 
TCGAAGCTCT GCGACATTTG CGAGGGTGAC GACTGCAAGA GCAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tep4 ( NM_001273661.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260590.1 Drosophila melanogaster Thioester-containing protein 4 (Tep4), transcript variant C, mRNA.

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CloneID	OFa16896
Clone ID Related Accession (Same CDS sequence)	NM_001273661.1
Accession Version	NM_001273661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Thioester-containing protein 4, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCGCG CAGACGCTTT TGCTGTCTCG CTTTGTGTGA TTTTGGCCTT GCTGCAGACG 
ATGGAACCGG TAAAAGCCGA GGGCAAGTAC ACCATTGTGG GTCCCGGAAC CATCCACTCC 
CACCGTGACT ACAATGTGGC CGTGGCTGTC CATCAGACCA AGGAGCCAGT CACCCTGAAA 
GTGGGCATCA CTGGACCCTC CTACAACAAA ACTGAGACCG TGGAACTAGC CACTGCCGGT 
GAGTTCAAGC AGATCACCTT CAAACTGCCC CCATTGGAGG CGGGTGAATA TAATCTGACG 
GCGGAGGGCG TCAAGGGACT GGAGTTCAAG AACTCCACCA AGCTGAACTG GGAGAATTTC 
AAGCCATACA TTAAAATCCA GACCGATAAG GGAAAGTACA AGCCCGGTGA TACTATCAAC 
TATCGTGTGA TCTTCCTGGA CGAGAACCTG CGGCCGGATA CCGCTAAGGA TGAAGTGGTG 
GTTTGGTTTG AGGACTCCAA AAGGAACCGC ATCAAGCAGG AGAAACACAT AAAGACCACC 
GGTGGCGTCT ACACTGGCAA ATTCGAGCTC TCCGAGTTCG CCACATTGGG CTCCTGGTCC 
CTTCATGTTC AGAATGGAGA TCAGCACCAC GACGGAGGTA TTTACTTTGG GGGTCGTAAA 
CAATTTGGAG GATTTGGTCA TCGCTGGCAT CGCTCGGATG AGCTCGTCAA CTTTGAGGTG 
GAGAAGTACG TGCTGCCCAA GTACTCCGTC AAAATGGATG CCACGCAGCA GGTTTCAGTG 
CGAGATGGCG AGTTCAATGT CGTCCTGAAG GCCAACTACA CCTATGGTAA GCCCGTGAAT 
GGTAAAGTTC TTGTCAATGT CCATCTGGAC TCGACGAGCA GTTGGGAGAA TGTGGACGGC 
AAGACAGTGC AAACGGACTA CCCCGGACAT TCTGTTGTTG GCACCGCCGA CATGGTCGGA 
GGCAAGGCCA AGTTGACCAT GGATCTCAAG GACTTCGCCA GTTATCTGCC GCACAAGACC 
TCTTCTTCAT ATGCCCAGAT TACGGCTACC GTGGAGGAAG ACTTCACCGG CGTGAAGCTC 
AATGAAACAG GCGGTGTGCA GCTGTACCCC TATCGGTATG AGATGTCCTG CACTGACTAC 
TCCAGTTGCT TCTCCTTCAA GCCCGACAAG GAACATGAAC TTAACTTCAA GATTACATAT 
GTCGACGGCT CACTAATTAC AGACACCAAG TCTGTTGTGA AGGCCAAGTT CACTGAAGGA 
ATTCGACGCA ATTATGCTTT CTATGCCTTT GGAACAGATC ATCAAGAACC GGAATTGCCA 
ACGATTGAGA AGAAAACGTT TGTGTTTGAA AGCCATTTGA ATGCGTCTGG TGTGGCGCCT 
TTCAAGGTGG TTCTTCCCGA TTTGCCGGAT ATCGCCAACT TTACTCGTTA CTATAGCATT 
GAGCTGGAGT TCGTCGATGA AAAACGAGAT CTGTACACCA CTTATCCCTA CAGGGAACCA 
AAACAGATTG AAAATCCTAG CTCTGAAGAG GAAAAGGAGT GGTTCAGGGC AGAAGTTCAG 
AGGCCCAAGG ACGTCTGGAA CCTCAAGATC GGGCAGGAGT ATCAAGTAAT CCTCAACTCC 
AGTCGTCCGC TCAAATACTT TGTGTACAAC ATTGTGGGAC GTGGAAACAT ATTGGAAACG 
AAACGTGTGG ACCTCGCGGA ACCCCAAACG ACCGTCAACG TAACCATTAA GCCCACTTTC 
CTCACTACTC CCTATGGTCG GGTGTACTTC TACTACGTGG ATGAGACAGG AGAGTTCCGA 
TATACCGAGG AAACATTCAG TGTGGAGGTC GAGCTGCAGA ACCAGATCGA AATCAAGGCT 
CCCGCGGAAG TTAAACCGGG TGCCGACGTT GCACTGGAGA TCAAGACGTC GCCAAAGTCA 
TTCGTTGGAT TGTTGGCCGT CGATCAGAGT GTCCTTCTGC TGGGCAGCAA CAACGATTTG 
AACAAGGAAT CCTTCAACTG GCGCCTAAAT GGCTATGACA CCTCAACTCC CTGGCAGGGT 
GGATACTCCT ACTACCCCGG AGAGCGAACT GGAGTCGTGA CTATGACCAA TGCCTATTTC 
TTCTATAATC GCACCGCCCC AGACTACAAT ATTCTTACAG AAGGATTTGG TGGAAGCTCT 
TTCGCCATGA GAAAGACTAC AGTGGCACAT GATAGTCATG TATTCCATTC CGGTGCTGGC 
GGACCAACTC AGGCCGTGGG ATTCTCCGCC GAAAGTGCGT CTGCATCTGC AGCACCAGTA 
GTGCGAAAGA ATTTCGCAGA GACCTGGATC TTTGCCGATA TCGAGAGCAC CGAGGAGGAG 
GTGTTCAAGT GGGTAAAGAC CATACCCGAT ACGATCACCA ACTGGGTGGT CACCGGCTTT 
TCACTGCATC CCCAGAAGGG ATTAGGTGTC ACCAATGATC AGACAAATAT CAAGACATTC 
CAGCCGTTCT TTGTCTCCGT TCGATTGCCA TACTCCGTGA AGCGAGGTGA GGTGATCAAT 
GTACCGGCAC TGGTCTTTAA CTACCTGCCC AAGACACTGG ACGTGGAACT GACCCTGGAC 
AACGAGGATC AAGAGTATGA CTTCGTGGAT GCCTCGAATG AGGTTATTGG TGACCAGAAG 
CGTACGCAGA ACATCAGAGT GGGCGCCAAC GAAGCGGCAG GAGCCTCATT TTTGATCCGA 
CCCAAGGTCA TTGGAAATAT TTTGCTGAAA TTCAAGGCCA TCTCACCGCT GGCCGGAGAT 
GCTATACACA AGCCGCTAAA GGTAGTGCCC GAAGGAATTA CCCAGTACCA GAACAGGGCA 
TTCTTTATCA ATCTTAAGGA TACGGGTGAG TTTAAGAACA CCTTCGAGCT GGAAGTGCCG 
GAGGATGTGG TCCCCGACTC CGAGCGTGTG GAATTCGGAT TGGTGGGTGA TCTCTTGGGA 
CCAGTGGTCA AGAACCTTGA GAACCTGTTG CGATTGCCCA GCGGTTGCGG TGAGCAGACC 
ATGTCCAAAT TGGTGCCCAA CTATCTGGTG AGGGATTATC TGAAGAGCAT CAAGAAACTC 
ACACCTGCAT TGGACACTCG CATCAAGAGG AATCTGCAGG ACGGATACCA ACATATGTTG 
CACTATCGCC ACGACGACGG CAGCTTCAGT TCCTTTGGGC CCACCAAATG GAGGCAGGAG 
GATCCAGTGC GCAATGGTTC CACCTGGCTG ACGGCCTATG TGCTGCGCTC GTTTAGCAAG 
ATCAAGGATA TAATTGACTT GGATGAGCAG ATCCTGGCCA AGGGCTACGA ATTCCTGTTA 
ACTCGACAGG CAGAAAATGG TAGCTTCACG GAACACGGAG AGTATTTCTA TAGCTCCCAG 
CGATCTCTAT TGACATTGAC CGCAAATTCC TTGCTCGCCC TGCTGGAGGA GGAGAAACCG 
AACCAGGCTG CCATTGACAA GGCGGTTGCC TATCTGAGCG CCAATACTGC CGAGTCCATT 
GAGCTCCTGC CCAAATCCAT TGCCATTTAT GCCCTGCAAA AGGCCAAGGC GCCGGAAGCT 
GCCAAGCAGG TGGCCAGTCT TAAGTCCCTG GCCAAACACG AGGACGATCG CACCTGGTGG 
ACCGAGGATC TGGATAAGCT GCGTGCCTCC AAGAACTGCG GACGCTGGTG GTGTTGGATC 
TGGAGTCAGG ATGTGGAGAT CACATCATAT GCGCTGCTTT CCCTTCTGGA TTCAGACCAG 
GAGACAGCCG ACTCTGTTCT GAACACCGTT CGCTGGTTGA TCGCCCAGCG TAATGGCTTC 
GGAGGATTTG CCTCCAGCCA GGACACGGTT GTCGGCCTCA CCGCCCTCAT TAAGTTCGCT 
GAGAAGTCGG GCTACGAGGC GGCCAAGTGG GAGGTGACCG TTTCCAACAA GGGAAAGCGT 
GAAAAGACCG AGAAGCTCAA CACATCGGAG GAAAATGACC TGCTCCTCCA GACAGTCGAG 
TTCCCACAGG GCACCAAGTC GCTGGAGTTT GAAGCCAAGG GAACTGGAGC TGCTATGGTC 
CAGATAAGCT ACCAATACAA CTTGGTGGAG AAGGAGCCGA AGCCGAGCTT TAAGATTCAG 
ACCACCGTTT TGCCGGAATC GTCGCCAGCC AATTTGGAAC TAAGCGTCTG CGTGGACTAC 
GTGGAGGAGG GCGAGTCCAA GGAATCCAAC ATGGCCATTC TGGAGGTATC CCTGCCGTCT 
GGTTATACTG CTGATGAGGA TAGCTTCGCC GACATCCGGA ACATCGAACG TGTGCGGTTG 
GTGGAAACCA AGAACGGTGA CTCCGTGGTG GTCATATACT TCGAGAATCT GGCCAAGAAC 
GAAGAAAAAT GCATTCGCAT AGAGGCGTAC AGAACACATG CGGTGGCCAA CCAGAAGCCA 
TCATCGGTGG TCCTCTACGA TTACTATGAC ACGAACAAGA AGGCCACCGA ATACTACTCC 
ATCAAGTCGA AGCTCTGCGA CATTTGCGAG GGTGACGACT GCAAGAGCAA GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260590.1
CDS	154..4650
Translation	MRRADAFAVSLCVILALLQTMEPVKAEGKYTIVGPGTIHSHRDYNVAVAVHQTKEPVTLKVGITGPSYNKTETVELATAGEFKQITFKLPPLEAGEYNLTAEGVKGLEFKNSTKLNWENFKPYIKIQTDKGKYKPGDTINYRVIFLDENLRPDTAKDEVVVWFEDSKRNRIKQEKHIKTTGGVYTGKFELSEFATLGSWSLHVQNGDQHHDGGIYFGGRKQFGGFGHRWHRSDELVNFEVEKYVLPKYSVKMDATQQVSVRDGEFNVVLKANYTYGKPVNGKVLVNVHLDSTSSWENVDGKTVQTDYPGHSVVGTADMVGGKAKLTMDLKDFASYLPHKTSSSYAQITATVEEDFTGVKLNETGGVQLYPYRYEMSCTDYSSCFSFKPDKEHELNFKITYVDGSLITDTKSVVKAKFTEGIRRNYAFYAFGTDHQEPELPTIEKKTFVFESHLNASGVAPFKVVLPDLPDIANFTRYYSIELEFVDEKRDLYTTYPYREPKQIENPSSEEEKEWFRAEVQRPKDVWNLKIGQEYQVILNSSRPLKYFVYNIVGRGNILETKRVDLAEPQTTVNVTIKPTFLTTPYGRVYFYYVDETGEFRYTEETFSVEVELQNQIEIKAPAEVKPGADVALEIKTSPKSFVGLLAVDQSVLLLGSNNDLNKESFNWRLNGYDTSTPWQGGYSYYPGERTGVVTMTNAYFFYNRTAPDYNILTEGFGGSSFAMRKTTVAHDSHVFHSGAGGPTQAVGFSAESASASAAPVVRKNFAETWIFADIESTEEEVFKWVKTIPDTITNWVVTGFSLHPQKGLGVTNDQTNIKTFQPFFVSVRLPYSVKRGEVINVPALVFNYLPKTLDVELTLDNEDQEYDFVDASNEVIGDQKRTQNIRVGANEAAGASFLIRPKVIGNILLKFKAISPLAGDAIHKPLKVVPEGITQYQNRAFFINLKDTGEFKNTFELEVPEDVVPDSERVEFGLVGDLLGPVVKNLENLLRLPSGCGEQTMSKLVPNYLVRDYLKSIKKLTPALDTRIKRNLQDGYQHMLHYRHDDGSFSSFGPTKWRQEDPVRNGSTWLTAYVLRSFSKIKDIIDLDEQILAKGYEFLLTRQAENGSFTEHGEYFYSSQRSLLTLTANSLLALLEEEKPNQAAIDKAVAYLSANTAESIELLPKSIAIYALQKAKAPEAAKQVASLKSLAKHEDDRTWWTEDLDKLRASKNCGRWWCWIWSQDVEITSYALLSLLDSDQETADSVLNTVRWLIAQRNGFGGFASSQDTVVGLTALIKFAEKSGYEAAKWEVTVSNKGKREKTEKLNTSEENDLLLQTVEFPQGTKSLEFEAKGTGAAMVQISYQYNLVEKEPKPSFKIQTTVLPESSPANLELSVCVDYVEEGESKESNMAILEVSLPSGYTADEDSFADIRNIERVRLVETKNGDSVVVIYFENLAKNEEKCIRIEAYRTHAVANQKPSSVVLYDYYDTNKKATEYYSIKSKLCDICEGDDCKSKC

Target ORF information:

RefSeq Version	NM_001273661.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Thioester-containing protein 4 (Tep4), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273661.1

ORF Insert Sequence:

ATGCGTCGCG CAGACGCTTT TGCTGTCTCG CTTTGTGTGA TTTTGGCCTT GCTGCAGACG 
ATGGAACCGG TAAAAGCCGA GGGCAAGTAC ACCATTGTGG GTCCCGGAAC CATCCACTCC 
CACCGTGACT ACAATGTGGC CGTGGCTGTC CATCAGACCA AGGAGCCAGT CACCCTGAAA 
GTGGGCATCA CTGGACCCTC CTACAACAAA ACTGAGACCG TGGAACTAGC CACTGCCGGT 
GAGTTCAAGC AGATCACCTT CAAACTGCCC CCATTGGAGG CGGGTGAATA TAATCTGACG 
GCGGAGGGCG TCAAGGGACT GGAGTTCAAG AACTCCACCA AGCTGAACTG GGAGAATTTC 
AAGCCATACA TTAAAATCCA GACCGATAAG GGAAAGTACA AGCCCGGTGA TACTATCAAC 
TATCGTGTGA TCTTCCTGGA CGAGAACCTG CGGCCGGATA CCGCTAAGGA TGAAGTGGTG 
GTTTGGTTTG AGGACTCCAA AAGGAACCGC ATCAAGCAGG AGAAACACAT AAAGACCACC 
GGTGGCGTCT ACACTGGCAA ATTCGAGCTC TCCGAGTTCG CCACATTGGG CTCCTGGTCC 
CTTCATGTTC AGAATGGAGA TCAGCACCAC GACGGAGGTA TTTACTTTGG GGGTCGTAAA 
CAATTTGGAG GATTTGGTCA TCGCTGGCAT CGCTCGGATG AGCTCGTCAA CTTTGAGGTG 
GAGAAGTACG TGCTGCCCAA GTACTCCGTC AAAATGGATG CCACGCAGCA GGTTTCAGTG 
CGAGATGGCG AGTTCAATGT CGTCCTGAAG GCCAACTACA CCTATGGTAA GCCCGTGAAT 
GGTAAAGTTC TTGTCAATGT CCATCTGGAC TCGACGAGCA GTTGGGAGAA TGTGGACGGC 
AAGACAGTGC AAACGGACTA CCCCGGACAT TCTGTTGTTG GCACCGCCGA CATGGTCGGA 
GGCAAGGCCA AGTTGACCAT GGATCTCAAG GACTTCGCCA GTTATCTGCC GCACAAGACC 
TCTTCTTCAT ATGCCCAGAT TACGGCTACC GTGGAGGAAG ACTTCACCGG CGTGAAGCTC 
AATGAAACAG GCGGTGTGCA GCTGTACCCC TATCGGTATG AGATGTCCTG CACTGACTAC 
TCCAGTTGCT TCTCCTTCAA GCCCGACAAG GAACATGAAC TTAACTTCAA GATTACATAT 
GTCGACGGCT CACTAATTAC AGACACCAAG TCTGTTGTGA AGGCCAAGTT CACTGAAGGA 
ATTCGACGCA ATTATGCTTT CTATGCCTTT GGAACAGATC ATCAAGAACC GGAATTGCCA 
ACGATTGAGA AGAAAACGTT TGTGTTTGAA AGCCATTTGA ATGCGTCTGG TGTGGCGCCT 
TTCAAGGTGG TTCTTCCCGA TTTGCCGGAT ATCGCCAACT TTACTCGTTA CTATAGCATT 
GAGCTGGAGT TCGTCGATGA AAAACGAGAT CTGTACACCA CTTATCCCTA CAGGGAACCA 
AAACAGATTG AAAATCCTAG CTCTGAAGAG GAAAAGGAGT GGTTCAGGGC AGAAGTTCAG 
AGGCCCAAGG ACGTCTGGAA CCTCAAGATC GGGCAGGAGT ATCAAGTAAT CCTCAACTCC 
AGTCGTCCGC TCAAATACTT TGTGTACAAC ATTGTGGGAC GTGGAAACAT ATTGGAAACG 
AAACGTGTGG ACCTCGCGGA ACCCCAAACG ACCGTCAACG TAACCATTAA GCCCACTTTC 
CTCACTACTC CCTATGGTCG GGTGTACTTC TACTACGTGG ATGAGACAGG AGAGTTCCGA 
TATACCGAGG AAACATTCAG TGTGGAGGTC GAGCTGCAGA ACCAGATCGA AATCAAGGCT 
CCCGCGGAAG TTAAACCGGG TGCCGACGTT GCACTGGAGA TCAAGACGTC GCCAAAGTCA 
TTCGTTGGAT TGTTGGCCGT CGATCAGAGT GTCCTTCTGC TGGGCAGCAA CAACGATTTG 
AACAAGGAAT CCTTCAACTG GCGCCTAAAT GGCTATGACA CCTCAACTCC CTGGCAGGGT 
GGATACTCCT ACTACCCCGG AGAGCGAACT GGAGTCGTGA CTATGACCAA TGCCTATTTC 
TTCTATAATC GCACCGCCCC AGACTACAAT ATTCTTACAG AAGGATTTGG TGGAAGCTCT 
TTCGCCATGA GAAAGACTAC AGTGGCACAT GATAGTCATG TATTCCATTC CGGTGCTGGC 
GGACCAACTC AGGCCGTGGG ATTCTCCGCC GAAAGTGCGT CTGCATCTGC AGCACCAGTA 
GTGCGAAAGA ATTTCGCAGA GACCTGGATC TTTGCCGATA TCGAGAGCAC CGAGGAGGAG 
GTGTTCAAGT GGGTAAAGAC CATACCCGAT ACGATCACCA ACTGGGTGGT CACCGGCTTT 
TCACTGCATC CCCAGAAGGG ATTAGGTGTC ACCAATGATC AGACAAATAT CAAGACATTC 
CAGCCGTTCT TTGTCTCCGT TCGATTGCCA TACTCCGTGA AGCGAGGTGA GGTGATCAAT 
GTACCGGCAC TGGTCTTTAA CTACCTGCCC AAGACACTGG ACGTGGAACT GACCCTGGAC 
AACGAGGATC AAGAGTATGA CTTCGTGGAT GCCTCGAATG AGGTTATTGG TGACCAGAAG 
CGTACGCAGA ACATCAGAGT GGGCGCCAAC GAAGCGGCAG GAGCCTCATT TTTGATCCGA 
CCCAAGGTCA TTGGAAATAT TTTGCTGAAA TTCAAGGCCA TCTCACCGCT GGCCGGAGAT 
GCTATACACA AGCCGCTAAA GGTAGTGCCC GAAGGAATTA CCCAGTACCA GAACAGGGCA 
TTCTTTATCA ATCTTAAGGA TACGGGTGAG TTTAAGAACA CCTTCGAGCT GGAAGTGCCG 
GAGGATGTGG TCCCCGACTC CGAGCGTGTG GAATTCGGAT TGGTGGGTGA TCTCTTGGGA 
CCAGTGGTCA AGAACCTTGA GAACCTGTTG CGATTGCCCA GCGGTTGCGG TGAGCAGACC 
ATGTCCAAAT TGGTGCCCAA CTATCTGGTG AGGGATTATC TGAAGAGCAT CAAGAAACTC 
ACACCTGCAT TGGACACTCG CATCAAGAGG AATCTGCAGG ACGGATACCA ACATATGTTG 
CACTATCGCC ACGACGACGG CAGCTTCAGT TCCTTTGGGC CCACCAAATG GAGGCAGGAG 
GATCCAGTGC GCAATGGTTC CACCTGGCTG ACGGCCTATG TGCTGCGCTC GTTTAGCAAG 
ATCAAGGATA TAATTGACTT GGATGAGCAG ATCCTGGCCA AGGGCTACGA ATTCCTGTTA 
ACTCGACAGG CAGAAAATGG TAGCTTCACG GAACACGGAG AGTATTTCTA TAGCTCCCAG 
CGATCTCTAT TGACATTGAC CGCAAATTCC TTGCTCGCCC TGCTGGAGGA GGAGAAACCG 
AACCAGGCTG CCATTGACAA GGCGGTTGCC TATCTGAGCG CCAATACTGC CGAGTCCATT 
GAGCTCCTGC CCAAATCCAT TGCCATTTAT GCCCTGCAAA AGGCCAAGGC GCCGGAAGCT 
GCCAAGCAGG TGGCCAGTCT TAAGTCCCTG GCCAAACACG AGGACGATCG CACCTGGTGG 
ACCGAGGATC TGGATAAGCT GCGTGCCTCC AAGAACTGCG GACGCTGGTG GTGTTGGATC 
TGGAGTCAGG ATGTGGAGAT CACATCATAT GCGCTGCTTT CCCTTCTGGA TTCAGACCAG 
GAGACAGCCG ACTCTGTTCT GAACACCGTT CGCTGGTTGA TCGCCCAGCG TAATGGCTTC 
GGAGGATTTG CCTCCAGCCA GGACACGGTT GTCGGCCTCA CCGCCCTCAT TAAGTTCGCT 
GAGAAGTCGG GCTACGAGGC GGCCAAGTGG GAGGTGACCG TTTCCAACAA GGGAAAGCGT 
GAAAAGACCG AGAAGCTCAA CACATCGGAG GAAAATGACC TGCTCCTCCA GACAGTCGAG 
TTCCCACAGG GCACCAAGTC GCTGGAGTTT GAAGCCAAGG GAACTGGAGC TGCTATGGTC 
CAGATAAGCT ACCAATACAA CTTGGTGGAG AAGGAGCCGA AGCCGAGCTT TAAGATTCAG 
ACCACCGTTT TGCCGGAATC GTCGCCAGCC AATTTGGAAC TAAGCGTCTG CGTGGACTAC 
GTGGAGGAGG GCGAGTCCAA GGAATCCAAC ATGGCCATTC TGGAGGTATC CCTGCCGTCT 
GGTTATACTG CTGATGAGGA TAGCTTCGCC GACATCCGGA ACATCGAACG TGTGCGGTTG 
GTGGAAACCA AGAACGGTGA CTCCGTGGTG GTCATATACT TCGAGAATCT GGCCAAGAAC 
GAAGAAAAAT GCATTCGCAT AGAGGCGTAC AGAACACATG CGGTGGCCAA CCAGAAGCCA 
TCATCGGTGG TCCTCTACGA TTACTATGAC ACGAACAAGA AGGCCACCGA ATACTACTCC 
ATCAAGTCGA AGCTCTGCGA CATTTGCGAG GGTGACGACT GCAAGAGCAA GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tep4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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