Top2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Top2
Entrez Gene ID	35225
Full Name	Topoisomerase 2
Synonyms	CG10223,CHRAC,Dmel\CG10223,TOP2,TOPO II,TOPOII,TopII,Topo II,TopoII,dCHRAC,dTopoII,suo,top2,topo II,topoII
General protein information	Preferred Names Topoisomerase 2 Names CG10223 gene product from transcript CG10223-RB CG10223-PA CG10223-PB DNA topoisomerase II DNA topoisomeriase II Top 2 Top2-PA Top2-PB Topoisomerase2 solofuso topoisomerase 2 topoisomerase II type II DNA topoisomerase type II topoisomerase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37E1-37E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057412.5	NP_476760.1	topoisomerase 2, isoform A
NM_001273651.1	NP_001260580.1	topoisomerase 2, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Top2a	NP_035753.2
Rattus norvegicus (Norway rat)	Top2a	NP_071519.2
Xenopus tropicalis (tropical clawed frog)	topoii	XP_002942632.2
Danio rerio (zebrafish)	top2a	NP_001003834.1
Drosophila melanogaster (fruit fly)	Top2	NP_001260580.1
Caenorhabditis elegans (roundworm)	top-2	NP_496536.1
Bos taurus (cattle)	TOP2A	XP_002696055.2
Gallus gallus (chicken)	TOP2A	NP_990122.1
Homo sapiens (human)	TOP2A	NP_001058.2
Pan troglodytes (chimpanzee)	TOP2A	XP_003315524.1
Canis lupus familiaris (dog)	TOP2A	XP_537646.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008917	XP_319665.4
	TOP2A	XP_002808175.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Top2-Mus101 interaction is important for maintaining the fidelity of chromosome segregation during mitosis.
Title: The C-terminal 20 Amino Acids of Drosophila Topoisomerase 2 Are Required for Binding to a BRCA1 C Terminus (BRCT) Domain-containing Protein, Mus101, and Fidelity of DNA Segregation.

our results suggest that the previously observed discrepancies in the chromosomal phenotypes elicited by Topo II downregulation in vertebrates might depend on slight differences in Topo II concentration and/or activity
Title: The analysis of mutant alleles of different strength reveals multiple functions of topoisomerase 2 in regulation of Drosophila chromosome structure.

These data suggest both that Topoisomerase II is involved in the resolution of heterochromatic DNA entanglements during meiosis I and that these entanglements must be resolved in order to complete meiosis.
Title: Topoisomerase II is required for the proper separation of heterochromatic regions during Drosophila melanogaster female meiosis.

Topo II is required to prevent Mod(mdg4)2.2 degradation and, consequently, to stabilize Su(Hw) insulator-mediated chromatin organization
Title: DNA topoisomerase II modulates insulator function in Drosophila.

Results suggest that displacement of the heterochromatin proteins D1, HP1 and topoisomerase II from satellite III result in stochastic chromatin opening and desilencing of the nearby white gene in white-mottled flies.
Title: Displacement of D1, HP1 and topoisomerase II from satellite heterochromatin by a specific polyamide.

The following Top2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14969	NM_057412.5 Latest version!	Drosophila melanogaster topoisomerase 2, transcript variant A (Top2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa14969	NM_001273651.1 Latest version!	Drosophila melanogaster topoisomerase 2, transcript variant B (Top2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Top2 ( NM_057412.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476760.1 Drosophila melanogaster topoisomerase 2, transcript variant A (Top2), mRNA.

Price & Availability↑

CloneID	OFa14969
Clone ID Related Accession (Same CDS sequence)	NM_057412.5 , NM_001273651.1
Accession Version	NM_057412.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057412.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACG GAAACAAGGC CCTGTCCATC GAACAGATGT ACCAGAAGAA GTCGCAGCTG 
GAGCACATCC TGCTGCGACC CGACTCGTAC ATCGGATCCG TGGAGTTCAC CAAGGAGCTG 
ATGTGGGTGT ATGACAACTC CCAAAACCGC ATGGTGCAGA AGGAGATCTC TTTCGTGCCC 
GGCCTCTACA AGATATTCGA CGAGATTCTT GTGAATGCGG CAGATAACAA GCAGCGAGAC 
AAGAGCATGA ACACCATTAA GATCGACATC GATCCGGAGC GCAATATGGT GTCCGTGTGG 
AACAACGGCC AGGGCATTCC GGTGACCATG CACAAGGAGC AGAAGATGTA CGTTCCAACG 
ATGATCTTTG GTCATCTGCT GACCTCGTCG AACTACAACG ACGATGAGAA GAAGGTCACT 
GGCGGCAGGA ACGGATACGG AGCGAAGCTC TGCAACATAT TCTCCACCAG CTTCACCGTT 
GAGACTGCCA CGAGGGAATA CAAGAAAAGT TTCAAGCAGA CTTGGGGAAA CAACATGGGA 
AAAGCCTCCG ATGTGCAGAT CAAGGACTTC AATGGCACTG ACTACACACG CATCACATTC 
AGTCCCGATC TGGCCAAGTT CAAGATGGAC CGTCTCGATG AAGATATTGT GGCTCTAATG 
TCGCGTCGCG CCTACGATGT GGCCGCCTCA TCCAAGGGAG TATCCGTCTT CTTAAACGGC 
AACAAACTGG GTGTACGCAA CTTCAAGGAC TATATTGATT TGCACATCAA GAACACGGAC 
GACGATTCTG GCCCACCGAT CAAGATAGTC CACGAGGTTG CCAACGAGCG TTGGGAGGTG 
GCTTGCTGTC CTTCAGATCG AGGCTTCCAA CAGGTCTCGT TTGTCAACTC GATAGCTACC 
TACAAGGGCG GTCGGCATGT GGACCATGTG GTAGACAATC TCATTAAGCA GCTGCTCGAG 
GTTCTGAAGA AAAAGAACAA AGGTGGCATC AACATCAAGC CATTCCAGGT CCGAAACCAT 
CTGTGGGTTT TTGTCAACTG CTTGATAGAG AACCCCACTT TCGACTCGCA GACCAAGGAG 
AACATGACTC TGCAACAAAA GGGCTTCGGT TCCAAGTGTA CCCTCTCGGA AAAGTTCATC 
AACAACATGT CCAAGTCTGG CATCGTGGAG TCTGTGCTGG CATGGGCCAA GTTCAAGGCC 
CAAAATGACA TTGCCAAGAC GGGCGGTCGC AAGTCAAGCA AGATCAAGGG CATTCCCAAG 
CTGGAGGACG CTAACGAGGC GGGTGGAAAG AACTCGATTA AATGCACCCT CATCCTCACC 
GAGGGAGACT CAGCCAAGTC ATTGGCCGTA TCCGGTTTGG GCGTGATCGG ACGAGATCTC 
TACGGCGTGT TCCCGCTTAG GGGTAAACTT CTAAATGTGC GGGAAGCTAA TTTCAAGCAG 
CTTTCGGAGA ATGCCGAAAT CAACAATTTA TGCAAGATAA TTGGCTTGCA ATACAAAAAG 
AAGTACCTCA CTGAGGACGA TCTAAAAACT CTGCGCTATG GCAAAGTGAT GATCATGACA 
GATCAGGATC AGGATGGCTC CCACATCAAG GGTCTCTTGA TCAACTTTAT CCACACCAAT 
TGGCCAGAGC TTCTGCGTCT GCCCTTCCTC GAGGAGTTCA TTACACCAAT TGTGAAGGCA 
ACCAAGAAAA ACGAGGAGCT GTCATTCTAC TCGCTACCCG AATTCGAGGA GTGGAAAAAT 
GATACAGCTA ATCACCATAC GTACAATATA AAGTACTATA AGGGTTTGGG TACTTCGACC 
TCCAAGGAGG CGAAAGAGTA TTTTCAAGAC ATGGATCGCC ATCGCATCTT ATTTAAGTAC 
GATGGATCGG TGGATGATGA GAGCATTGTT ATGGCCTTCT CCAAGAAGCA CATCGAGTCG 
CGAAAGGTAT GGCTTACCAA CCACATGGAC GAGGTGAAGC GTCGCAAGGA GCTCGGTTTG 
CCAGAGCGAT ATCTCTACAC CAAGGGCACC AAGAGCATCA CCTATGCAGA CTTTATCAAT 
CTGGAGTTGG TGCTGTTCTC GAATGCTGAC AATGAGCGCT CCATTCCTAG TCTGGTGGAT 
GGCTTGAAGC CGGGTCAGCG GAAGGTGATG TTCACTTGCT TCAAGAGGAA TGACAAGCGT 
GAGGTGAAGG TGGCCCAGCT ATCCGGATCA GTGGCGGAGA TGTCAGCCTA TCACCACGGA 
GAGGTATCCC TACAGATGAC AATCGTGAAT CTGGCTCAGA ATTTTGTGGG TGCCAACAAC 
ATTAATCTGC TTGAGCCACG CGGTCAATTT GGTACCCGCT TGTCGGGAGG CAAAGATTGC 
GCCAGCGCTC GTTACATTTT CACTATAATG TCTCCCTTGA CGCGACTCAT CTACCATCCT 
TTGGACGATC CACTTTTGGA TTACCAGGTG GACGATGGCC AAAAGATCGA GCCACTATGG 
TATCTGCCCA TCATACCGAT GGTATTGGTA AACGGAGCCG AGGGCATAGG AACTGGATGG 
TCCACGAAGA TATCCAACTA CAATCCTCGT GAGATTATGA AAAATCTAAG GAAGATGATA 
AACGGACAAG AGCCAAGTGT GATGCATCCG TGGTACAAGA ACTTTTTAGG ACGCATGGAG 
TATGTTTCGG ATGGTCGTTA TATTCAGACT GGTAACATTC AAATTTTGTC CGGAAACCGT 
TTAGAAATCA GTGAACTCCC TGTGGGCGTA TGGACGCAAA ACTACAAGGA AAATGTCCTG 
GAGCCTTTAT CAAACGGCAC CGAAAAGGTT AAGGGTATTA TTTCCGAGTA CAGGGAGTAT 
CATACAGACA CCACCGTTCG CTTTGTGATC AGTTTCGCAC CTGGAGAATT TGAGCGCATT 
CATGCAGAAG AAGGTGGTTT CTACCGAGTG TTCAAACTTA CCACGACGCT GTCCACCAAC 
CAGATGCATG CGTTCGACCA GAACAACTGT CTGCGACGCT TCCCCACCGC GATCGATATC 
CTTAAAGAGT ATTACAAACT GCGACGAGAG TACTACGCCC GCCGGAGGGA CTTTTTGGTC 
GGCCAGCTCA CGGCGCAGGC TGATCGCCTC AGTGATCAGG CGCGCTTTAT TCTCGAAAAG 
TGTGAGAAGA AGCTGGTGGT GGAGAACAAG CAGCGCAAGG CCATGTGTGA TGAGTTGTTG 
AAGCGTGGAT ATCGTCCCGA TCCCGTCAAG GAGTGGCAGC GCCGCATTAA AATGGAGGAT 
GCCGAGCAAG CTGACGAAGA GGACGAAGAA GAAGAGGAGG CAGCTCCCAG TGTCAGCTCA 
AAGGCTAAGA AGGAAAAGGA AGTCGATCCG GAAAAGGCCT TTAAGAAACT AACTGATGTC 
AAAAAGTTTG ACTACCTTCT GGGTATGTCC ATGTGGATGT TGACAGAGGA GAAGAAAAAC 
GAGCTACTCA AACAGCGCGA CACTAAACTG TCCGAGTTGG AAAGTCTGCG CAAGAAGACG 
CCGGAGATGC TTTGGCTGGA TGATTTGGAT GCCCTCGAAT CGAAGTTAAA TGAAGTAGAG 
GAGAAGGAGC GCGCAGAAGA GCAGGGGATC AATCTCAAGA CCGCGAAGGC TTTGAAGGGC 
CAGAAGTCGG CCTCAGCTAA GGGCAGAAAG GTTAAGTCAA TGGGAGGCGG AGCGGGTGCC 
GGAGACGTAT TCCCAGATCC CGATGGAGAA CCCGTCGAGT TTAAGATCAC CGAAGAAATC 
ATTAAGAAAA TGGCAGCGGC TGCCAAGGTA GCTCAGGCGG CTAAGGAGCC GAAGAAACCC 
AAAGAGCCCA AGGAGCCAAA AGTGAAGAAG GAACCGAAGG GCAAGCAGAT TAAAGCCGAA 
CCTGATGCCA GCGGCGATGA GGTGGACGAA TTTGATGCAA TGGTCGAGGG CGGCTCTAAG 
ACCTCACCCA AGGCCAAGAA GGCGGTTGTC AAGAAGGAGC CGGGAGAAAA AAAGCCGCGC 
CAAAAGAAGG AGAACGGCGG CGGGCTCAAG CAGAGCAAGA TTGACTTCAG CAAGGCCAAG 
GCGAAAAAGA GCGACGATGA CGTGGAGGAA GTAACTCCTC GTGCAGAGCG TCCTGGACGT 
CGACAGGCCA GTAAGAAGAT AGACTACAGC TCGCTCTTCT CGGATGAGGA GGAAGACGGT 
GGCAACGTTG GCTCTGATGA TGATGGCAAT GCCAGCGATG ATGATTCGCC CAAGCGTCCA 
GCTAAGCGTG GACGAGAGGA TGAGAGCAGT GGAGGAGCCA AAAAGAAAGC TCCGCCCAAG 
AAGAGGCGAG CTGTAATTGA AAGCGATGAT GATGATATCG AGATTGATGA AGATGATGAT 
GATGATTCTG ATTTTAATTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476760.1
CDS	271..4614
Translation	MENGNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFDEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTRITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDDSGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNKGGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESVLAWAKFKAQNDIAKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDEVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISNYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVWTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTTLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKCEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKEKEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLDALESKLNEVEEKERAEEQGINLKTAKALKGQKSASAKGRKVKSMGGGAGAGDVFPDPDGEPVEFKITEEIIKKMAAAAKVAQAAKEPKKPKEPKEPKVKKEPKGKQIKAEPDASGDEVDEFDAMVEGGSKTSPKAKKAVVKKEPGEKKPRQKKENGGGLKQSKIDFSKAKAKKSDDDVEEVTPRAERPGRRQASKKIDYSSLFSDEEEDGGNVGSDDDGNASDDDSPKRPAKRGREDESSGGAKKKAPPKKRRAVIESDDDDIEIDEDDDDDSDFNC

Target ORF information:

RefSeq Version	NM_057412.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase 2, transcript variant A (Top2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057412.5

ORF Insert Sequence:

ATGGAGAACG GAAACAAGGC CCTGTCCATC GAACAGATGT ACCAGAAGAA GTCGCAGCTG 
GAGCACATCC TGCTGCGACC CGACTCGTAC ATCGGATCCG TGGAGTTCAC CAAGGAGCTG 
ATGTGGGTGT ATGACAACTC CCAAAACCGC ATGGTGCAGA AGGAGATCTC TTTCGTGCCC 
GGCCTCTACA AGATATTCGA CGAGATTCTT GTGAATGCGG CAGATAACAA GCAGCGAGAC 
AAGAGCATGA ACACCATTAA GATCGACATC GATCCGGAGC GCAATATGGT GTCCGTGTGG 
AACAACGGCC AGGGCATTCC GGTGACCATG CACAAGGAGC AGAAGATGTA CGTTCCAACG 
ATGATCTTTG GTCATCTGCT GACCTCGTCG AACTACAACG ACGATGAGAA GAAGGTCACT 
GGCGGCAGGA ACGGATACGG AGCGAAGCTC TGCAACATAT TCTCCACCAG CTTCACCGTT 
GAGACTGCCA CGAGGGAATA CAAGAAAAGT TTCAAGCAGA CTTGGGGAAA CAACATGGGA 
AAAGCCTCCG ATGTGCAGAT CAAGGACTTC AATGGCACTG ACTACACACG CATCACATTC 
AGTCCCGATC TGGCCAAGTT CAAGATGGAC CGTCTCGATG AAGATATTGT GGCTCTAATG 
TCGCGTCGCG CCTACGATGT GGCCGCCTCA TCCAAGGGAG TATCCGTCTT CTTAAACGGC 
AACAAACTGG GTGTACGCAA CTTCAAGGAC TATATTGATT TGCACATCAA GAACACGGAC 
GACGATTCTG GCCCACCGAT CAAGATAGTC CACGAGGTTG CCAACGAGCG TTGGGAGGTG 
GCTTGCTGTC CTTCAGATCG AGGCTTCCAA CAGGTCTCGT TTGTCAACTC GATAGCTACC 
TACAAGGGCG GTCGGCATGT GGACCATGTG GTAGACAATC TCATTAAGCA GCTGCTCGAG 
GTTCTGAAGA AAAAGAACAA AGGTGGCATC AACATCAAGC CATTCCAGGT CCGAAACCAT 
CTGTGGGTTT TTGTCAACTG CTTGATAGAG AACCCCACTT TCGACTCGCA GACCAAGGAG 
AACATGACTC TGCAACAAAA GGGCTTCGGT TCCAAGTGTA CCCTCTCGGA AAAGTTCATC 
AACAACATGT CCAAGTCTGG CATCGTGGAG TCTGTGCTGG CATGGGCCAA GTTCAAGGCC 
CAAAATGACA TTGCCAAGAC GGGCGGTCGC AAGTCAAGCA AGATCAAGGG CATTCCCAAG 
CTGGAGGACG CTAACGAGGC GGGTGGAAAG AACTCGATTA AATGCACCCT CATCCTCACC 
GAGGGAGACT CAGCCAAGTC ATTGGCCGTA TCCGGTTTGG GCGTGATCGG ACGAGATCTC 
TACGGCGTGT TCCCGCTTAG GGGTAAACTT CTAAATGTGC GGGAAGCTAA TTTCAAGCAG 
CTTTCGGAGA ATGCCGAAAT CAACAATTTA TGCAAGATAA TTGGCTTGCA ATACAAAAAG 
AAGTACCTCA CTGAGGACGA TCTAAAAACT CTGCGCTATG GCAAAGTGAT GATCATGACA 
GATCAGGATC AGGATGGCTC CCACATCAAG GGTCTCTTGA TCAACTTTAT CCACACCAAT 
TGGCCAGAGC TTCTGCGTCT GCCCTTCCTC GAGGAGTTCA TTACACCAAT TGTGAAGGCA 
ACCAAGAAAA ACGAGGAGCT GTCATTCTAC TCGCTACCCG AATTCGAGGA GTGGAAAAAT 
GATACAGCTA ATCACCATAC GTACAATATA AAGTACTATA AGGGTTTGGG TACTTCGACC 
TCCAAGGAGG CGAAAGAGTA TTTTCAAGAC ATGGATCGCC ATCGCATCTT ATTTAAGTAC 
GATGGATCGG TGGATGATGA GAGCATTGTT ATGGCCTTCT CCAAGAAGCA CATCGAGTCG 
CGAAAGGTAT GGCTTACCAA CCACATGGAC GAGGTGAAGC GTCGCAAGGA GCTCGGTTTG 
CCAGAGCGAT ATCTCTACAC CAAGGGCACC AAGAGCATCA CCTATGCAGA CTTTATCAAT 
CTGGAGTTGG TGCTGTTCTC GAATGCTGAC AATGAGCGCT CCATTCCTAG TCTGGTGGAT 
GGCTTGAAGC CGGGTCAGCG GAAGGTGATG TTCACTTGCT TCAAGAGGAA TGACAAGCGT 
GAGGTGAAGG TGGCCCAGCT ATCCGGATCA GTGGCGGAGA TGTCAGCCTA TCACCACGGA 
GAGGTATCCC TACAGATGAC AATCGTGAAT CTGGCTCAGA ATTTTGTGGG TGCCAACAAC 
ATTAATCTGC TTGAGCCACG CGGTCAATTT GGTACCCGCT TGTCGGGAGG CAAAGATTGC 
GCCAGCGCTC GTTACATTTT CACTATAATG TCTCCCTTGA CGCGACTCAT CTACCATCCT 
TTGGACGATC CACTTTTGGA TTACCAGGTG GACGATGGCC AAAAGATCGA GCCACTATGG 
TATCTGCCCA TCATACCGAT GGTATTGGTA AACGGAGCCG AGGGCATAGG AACTGGATGG 
TCCACGAAGA TATCCAACTA CAATCCTCGT GAGATTATGA AAAATCTAAG GAAGATGATA 
AACGGACAAG AGCCAAGTGT GATGCATCCG TGGTACAAGA ACTTTTTAGG ACGCATGGAG 
TATGTTTCGG ATGGTCGTTA TATTCAGACT GGTAACATTC AAATTTTGTC CGGAAACCGT 
TTAGAAATCA GTGAACTCCC TGTGGGCGTA TGGACGCAAA ACTACAAGGA AAATGTCCTG 
GAGCCTTTAT CAAACGGCAC CGAAAAGGTT AAGGGTATTA TTTCCGAGTA CAGGGAGTAT 
CATACAGACA CCACCGTTCG CTTTGTGATC AGTTTCGCAC CTGGAGAATT TGAGCGCATT 
CATGCAGAAG AAGGTGGTTT CTACCGAGTG TTCAAACTTA CCACGACGCT GTCCACCAAC 
CAGATGCATG CGTTCGACCA GAACAACTGT CTGCGACGCT TCCCCACCGC GATCGATATC 
CTTAAAGAGT ATTACAAACT GCGACGAGAG TACTACGCCC GCCGGAGGGA CTTTTTGGTC 
GGCCAGCTCA CGGCGCAGGC TGATCGCCTC AGTGATCAGG CGCGCTTTAT TCTCGAAAAG 
TGTGAGAAGA AGCTGGTGGT GGAGAACAAG CAGCGCAAGG CCATGTGTGA TGAGTTGTTG 
AAGCGTGGAT ATCGTCCCGA TCCCGTCAAG GAGTGGCAGC GCCGCATTAA AATGGAGGAT 
GCCGAGCAAG CTGACGAAGA GGACGAAGAA GAAGAGGAGG CAGCTCCCAG TGTCAGCTCA 
AAGGCTAAGA AGGAAAAGGA AGTCGATCCG GAAAAGGCCT TTAAGAAACT AACTGATGTC 
AAAAAGTTTG ACTACCTTCT GGGTATGTCC ATGTGGATGT TGACAGAGGA GAAGAAAAAC 
GAGCTACTCA AACAGCGCGA CACTAAACTG TCCGAGTTGG AAAGTCTGCG CAAGAAGACG 
CCGGAGATGC TTTGGCTGGA TGATTTGGAT GCCCTCGAAT CGAAGTTAAA TGAAGTAGAG 
GAGAAGGAGC GCGCAGAAGA GCAGGGGATC AATCTCAAGA CCGCGAAGGC TTTGAAGGGC 
CAGAAGTCGG CCTCAGCTAA GGGCAGAAAG GTTAAGTCAA TGGGAGGCGG AGCGGGTGCC 
GGAGACGTAT TCCCAGATCC CGATGGAGAA CCCGTCGAGT TTAAGATCAC CGAAGAAATC 
ATTAAGAAAA TGGCAGCGGC TGCCAAGGTA GCTCAGGCGG CTAAGGAGCC GAAGAAACCC 
AAAGAGCCCA AGGAGCCAAA AGTGAAGAAG GAACCGAAGG GCAAGCAGAT TAAAGCCGAA 
CCTGATGCCA GCGGCGATGA GGTGGACGAA TTTGATGCAA TGGTCGAGGG CGGCTCTAAG 
ACCTCACCCA AGGCCAAGAA GGCGGTTGTC AAGAAGGAGC CGGGAGAAAA AAAGCCGCGC 
CAAAAGAAGG AGAACGGCGG CGGGCTCAAG CAGAGCAAGA TTGACTTCAG CAAGGCCAAG 
GCGAAAAAGA GCGACGATGA CGTGGAGGAA GTAACTCCTC GTGCAGAGCG TCCTGGACGT 
CGACAGGCCA GTAAGAAGAT AGACTACAGC TCGCTCTTCT CGGATGAGGA GGAAGACGGT 
GGCAACGTTG GCTCTGATGA TGATGGCAAT GCCAGCGATG ATGATTCGCC CAAGCGTCCA 
GCTAAGCGTG GACGAGAGGA TGAGAGCAGT GGAGGAGCCA AAAAGAAAGC TCCGCCCAAG 
AAGAGGCGAG CTGTAATTGA AAGCGATGAT GATGATATCG AGATTGATGA AGATGATGAT 
GATGATTCTG ATTTTAATTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Top2 ( NM_001273651.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260580.1 Drosophila melanogaster topoisomerase 2, transcript variant B (Top2), mRNA.

Price & Availability↑

CloneID	OFa14969
Clone ID Related Accession (Same CDS sequence)	NM_057412.5 , NM_001273651.1
Accession Version	NM_001273651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACG GAAACAAGGC CCTGTCCATC GAACAGATGT ACCAGAAGAA GTCGCAGCTG 
GAGCACATCC TGCTGCGACC CGACTCGTAC ATCGGATCCG TGGAGTTCAC CAAGGAGCTG 
ATGTGGGTGT ATGACAACTC CCAAAACCGC ATGGTGCAGA AGGAGATCTC TTTCGTGCCC 
GGCCTCTACA AGATATTCGA CGAGATTCTT GTGAATGCGG CAGATAACAA GCAGCGAGAC 
AAGAGCATGA ACACCATTAA GATCGACATC GATCCGGAGC GCAATATGGT GTCCGTGTGG 
AACAACGGCC AGGGCATTCC GGTGACCATG CACAAGGAGC AGAAGATGTA CGTTCCAACG 
ATGATCTTTG GTCATCTGCT GACCTCGTCG AACTACAACG ACGATGAGAA GAAGGTCACT 
GGCGGCAGGA ACGGATACGG AGCGAAGCTC TGCAACATAT TCTCCACCAG CTTCACCGTT 
GAGACTGCCA CGAGGGAATA CAAGAAAAGT TTCAAGCAGA CTTGGGGAAA CAACATGGGA 
AAAGCCTCCG ATGTGCAGAT CAAGGACTTC AATGGCACTG ACTACACACG CATCACATTC 
AGTCCCGATC TGGCCAAGTT CAAGATGGAC CGTCTCGATG AAGATATTGT GGCTCTAATG 
TCGCGTCGCG CCTACGATGT GGCCGCCTCA TCCAAGGGAG TATCCGTCTT CTTAAACGGC 
AACAAACTGG GTGTACGCAA CTTCAAGGAC TATATTGATT TGCACATCAA GAACACGGAC 
GACGATTCTG GCCCACCGAT CAAGATAGTC CACGAGGTTG CCAACGAGCG TTGGGAGGTG 
GCTTGCTGTC CTTCAGATCG AGGCTTCCAA CAGGTCTCGT TTGTCAACTC GATAGCTACC 
TACAAGGGCG GTCGGCATGT GGACCATGTG GTAGACAATC TCATTAAGCA GCTGCTCGAG 
GTTCTGAAGA AAAAGAACAA AGGTGGCATC AACATCAAGC CATTCCAGGT CCGAAACCAT 
CTGTGGGTTT TTGTCAACTG CTTGATAGAG AACCCCACTT TCGACTCGCA GACCAAGGAG 
AACATGACTC TGCAACAAAA GGGCTTCGGT TCCAAGTGTA CCCTCTCGGA AAAGTTCATC 
AACAACATGT CCAAGTCTGG CATCGTGGAG TCTGTGCTGG CATGGGCCAA GTTCAAGGCC 
CAAAATGACA TTGCCAAGAC GGGCGGTCGC AAGTCAAGCA AGATCAAGGG CATTCCCAAG 
CTGGAGGACG CTAACGAGGC GGGTGGAAAG AACTCGATTA AATGCACCCT CATCCTCACC 
GAGGGAGACT CAGCCAAGTC ATTGGCCGTA TCCGGTTTGG GCGTGATCGG ACGAGATCTC 
TACGGCGTGT TCCCGCTTAG GGGTAAACTT CTAAATGTGC GGGAAGCTAA TTTCAAGCAG 
CTTTCGGAGA ATGCCGAAAT CAACAATTTA TGCAAGATAA TTGGCTTGCA ATACAAAAAG 
AAGTACCTCA CTGAGGACGA TCTAAAAACT CTGCGCTATG GCAAAGTGAT GATCATGACA 
GATCAGGATC AGGATGGCTC CCACATCAAG GGTCTCTTGA TCAACTTTAT CCACACCAAT 
TGGCCAGAGC TTCTGCGTCT GCCCTTCCTC GAGGAGTTCA TTACACCAAT TGTGAAGGCA 
ACCAAGAAAA ACGAGGAGCT GTCATTCTAC TCGCTACCCG AATTCGAGGA GTGGAAAAAT 
GATACAGCTA ATCACCATAC GTACAATATA AAGTACTATA AGGGTTTGGG TACTTCGACC 
TCCAAGGAGG CGAAAGAGTA TTTTCAAGAC ATGGATCGCC ATCGCATCTT ATTTAAGTAC 
GATGGATCGG TGGATGATGA GAGCATTGTT ATGGCCTTCT CCAAGAAGCA CATCGAGTCG 
CGAAAGGTAT GGCTTACCAA CCACATGGAC GAGGTGAAGC GTCGCAAGGA GCTCGGTTTG 
CCAGAGCGAT ATCTCTACAC CAAGGGCACC AAGAGCATCA CCTATGCAGA CTTTATCAAT 
CTGGAGTTGG TGCTGTTCTC GAATGCTGAC AATGAGCGCT CCATTCCTAG TCTGGTGGAT 
GGCTTGAAGC CGGGTCAGCG GAAGGTGATG TTCACTTGCT TCAAGAGGAA TGACAAGCGT 
GAGGTGAAGG TGGCCCAGCT ATCCGGATCA GTGGCGGAGA TGTCAGCCTA TCACCACGGA 
GAGGTATCCC TACAGATGAC AATCGTGAAT CTGGCTCAGA ATTTTGTGGG TGCCAACAAC 
ATTAATCTGC TTGAGCCACG CGGTCAATTT GGTACCCGCT TGTCGGGAGG CAAAGATTGC 
GCCAGCGCTC GTTACATTTT CACTATAATG TCTCCCTTGA CGCGACTCAT CTACCATCCT 
TTGGACGATC CACTTTTGGA TTACCAGGTG GACGATGGCC AAAAGATCGA GCCACTATGG 
TATCTGCCCA TCATACCGAT GGTATTGGTA AACGGAGCCG AGGGCATAGG AACTGGATGG 
TCCACGAAGA TATCCAACTA CAATCCTCGT GAGATTATGA AAAATCTAAG GAAGATGATA 
AACGGACAAG AGCCAAGTGT GATGCATCCG TGGTACAAGA ACTTTTTAGG ACGCATGGAG 
TATGTTTCGG ATGGTCGTTA TATTCAGACT GGTAACATTC AAATTTTGTC CGGAAACCGT 
TTAGAAATCA GTGAACTCCC TGTGGGCGTA TGGACGCAAA ACTACAAGGA AAATGTCCTG 
GAGCCTTTAT CAAACGGCAC CGAAAAGGTT AAGGGTATTA TTTCCGAGTA CAGGGAGTAT 
CATACAGACA CCACCGTTCG CTTTGTGATC AGTTTCGCAC CTGGAGAATT TGAGCGCATT 
CATGCAGAAG AAGGTGGTTT CTACCGAGTG TTCAAACTTA CCACGACGCT GTCCACCAAC 
CAGATGCATG CGTTCGACCA GAACAACTGT CTGCGACGCT TCCCCACCGC GATCGATATC 
CTTAAAGAGT ATTACAAACT GCGACGAGAG TACTACGCCC GCCGGAGGGA CTTTTTGGTC 
GGCCAGCTCA CGGCGCAGGC TGATCGCCTC AGTGATCAGG CGCGCTTTAT TCTCGAAAAG 
TGTGAGAAGA AGCTGGTGGT GGAGAACAAG CAGCGCAAGG CCATGTGTGA TGAGTTGTTG 
AAGCGTGGAT ATCGTCCCGA TCCCGTCAAG GAGTGGCAGC GCCGCATTAA AATGGAGGAT 
GCCGAGCAAG CTGACGAAGA GGACGAAGAA GAAGAGGAGG CAGCTCCCAG TGTCAGCTCA 
AAGGCTAAGA AGGAAAAGGA AGTCGATCCG GAAAAGGCCT TTAAGAAACT AACTGATGTC 
AAAAAGTTTG ACTACCTTCT GGGTATGTCC ATGTGGATGT TGACAGAGGA GAAGAAAAAC 
GAGCTACTCA AACAGCGCGA CACTAAACTG TCCGAGTTGG AAAGTCTGCG CAAGAAGACG 
CCGGAGATGC TTTGGCTGGA TGATTTGGAT GCCCTCGAAT CGAAGTTAAA TGAAGTAGAG 
GAGAAGGAGC GCGCAGAAGA GCAGGGGATC AATCTCAAGA CCGCGAAGGC TTTGAAGGGC 
CAGAAGTCGG CCTCAGCTAA GGGCAGAAAG GTTAAGTCAA TGGGAGGCGG AGCGGGTGCC 
GGAGACGTAT TCCCAGATCC CGATGGAGAA CCCGTCGAGT TTAAGATCAC CGAAGAAATC 
ATTAAGAAAA TGGCAGCGGC TGCCAAGGTA GCTCAGGCGG CTAAGGAGCC GAAGAAACCC 
AAAGAGCCCA AGGAGCCAAA AGTGAAGAAG GAACCGAAGG GCAAGCAGAT TAAAGCCGAA 
CCTGATGCCA GCGGCGATGA GGTGGACGAA TTTGATGCAA TGGTCGAGGG CGGCTCTAAG 
ACCTCACCCA AGGCCAAGAA GGCGGTTGTC AAGAAGGAGC CGGGAGAAAA AAAGCCGCGC 
CAAAAGAAGG AGAACGGCGG CGGGCTCAAG CAGAGCAAGA TTGACTTCAG CAAGGCCAAG 
GCGAAAAAGA GCGACGATGA CGTGGAGGAA GTAACTCCTC GTGCAGAGCG TCCTGGACGT 
CGACAGGCCA GTAAGAAGAT AGACTACAGC TCGCTCTTCT CGGATGAGGA GGAAGACGGT 
GGCAACGTTG GCTCTGATGA TGATGGCAAT GCCAGCGATG ATGATTCGCC CAAGCGTCCA 
GCTAAGCGTG GACGAGAGGA TGAGAGCAGT GGAGGAGCCA AAAAGAAAGC TCCGCCCAAG 
AAGAGGCGAG CTGTAATTGA AAGCGATGAT GATGATATCG AGATTGATGA AGATGATGAT 
GATGATTCTG ATTTTAATTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260580.1
CDS	526..4869
Translation	MENGNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFDEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTRITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDDSGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNKGGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESVLAWAKFKAQNDIAKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDEVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISNYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVWTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTTLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKCEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKEKEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLDALESKLNEVEEKERAEEQGINLKTAKALKGQKSASAKGRKVKSMGGGAGAGDVFPDPDGEPVEFKITEEIIKKMAAAAKVAQAAKEPKKPKEPKEPKVKKEPKGKQIKAEPDASGDEVDEFDAMVEGGSKTSPKAKKAVVKKEPGEKKPRQKKENGGGLKQSKIDFSKAKAKKSDDDVEEVTPRAERPGRRQASKKIDYSSLFSDEEEDGGNVGSDDDGNASDDDSPKRPAKRGREDESSGGAKKKAPPKKRRAVIESDDDDIEIDEDDDDDSDFNC

Target ORF information:

RefSeq Version	NM_001273651.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase 2, transcript variant B (Top2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273651.1

ORF Insert Sequence:

ATGGAGAACG GAAACAAGGC CCTGTCCATC GAACAGATGT ACCAGAAGAA GTCGCAGCTG 
GAGCACATCC TGCTGCGACC CGACTCGTAC ATCGGATCCG TGGAGTTCAC CAAGGAGCTG 
ATGTGGGTGT ATGACAACTC CCAAAACCGC ATGGTGCAGA AGGAGATCTC TTTCGTGCCC 
GGCCTCTACA AGATATTCGA CGAGATTCTT GTGAATGCGG CAGATAACAA GCAGCGAGAC 
AAGAGCATGA ACACCATTAA GATCGACATC GATCCGGAGC GCAATATGGT GTCCGTGTGG 
AACAACGGCC AGGGCATTCC GGTGACCATG CACAAGGAGC AGAAGATGTA CGTTCCAACG 
ATGATCTTTG GTCATCTGCT GACCTCGTCG AACTACAACG ACGATGAGAA GAAGGTCACT 
GGCGGCAGGA ACGGATACGG AGCGAAGCTC TGCAACATAT TCTCCACCAG CTTCACCGTT 
GAGACTGCCA CGAGGGAATA CAAGAAAAGT TTCAAGCAGA CTTGGGGAAA CAACATGGGA 
AAAGCCTCCG ATGTGCAGAT CAAGGACTTC AATGGCACTG ACTACACACG CATCACATTC 
AGTCCCGATC TGGCCAAGTT CAAGATGGAC CGTCTCGATG AAGATATTGT GGCTCTAATG 
TCGCGTCGCG CCTACGATGT GGCCGCCTCA TCCAAGGGAG TATCCGTCTT CTTAAACGGC 
AACAAACTGG GTGTACGCAA CTTCAAGGAC TATATTGATT TGCACATCAA GAACACGGAC 
GACGATTCTG GCCCACCGAT CAAGATAGTC CACGAGGTTG CCAACGAGCG TTGGGAGGTG 
GCTTGCTGTC CTTCAGATCG AGGCTTCCAA CAGGTCTCGT TTGTCAACTC GATAGCTACC 
TACAAGGGCG GTCGGCATGT GGACCATGTG GTAGACAATC TCATTAAGCA GCTGCTCGAG 
GTTCTGAAGA AAAAGAACAA AGGTGGCATC AACATCAAGC CATTCCAGGT CCGAAACCAT 
CTGTGGGTTT TTGTCAACTG CTTGATAGAG AACCCCACTT TCGACTCGCA GACCAAGGAG 
AACATGACTC TGCAACAAAA GGGCTTCGGT TCCAAGTGTA CCCTCTCGGA AAAGTTCATC 
AACAACATGT CCAAGTCTGG CATCGTGGAG TCTGTGCTGG CATGGGCCAA GTTCAAGGCC 
CAAAATGACA TTGCCAAGAC GGGCGGTCGC AAGTCAAGCA AGATCAAGGG CATTCCCAAG 
CTGGAGGACG CTAACGAGGC GGGTGGAAAG AACTCGATTA AATGCACCCT CATCCTCACC 
GAGGGAGACT CAGCCAAGTC ATTGGCCGTA TCCGGTTTGG GCGTGATCGG ACGAGATCTC 
TACGGCGTGT TCCCGCTTAG GGGTAAACTT CTAAATGTGC GGGAAGCTAA TTTCAAGCAG 
CTTTCGGAGA ATGCCGAAAT CAACAATTTA TGCAAGATAA TTGGCTTGCA ATACAAAAAG 
AAGTACCTCA CTGAGGACGA TCTAAAAACT CTGCGCTATG GCAAAGTGAT GATCATGACA 
GATCAGGATC AGGATGGCTC CCACATCAAG GGTCTCTTGA TCAACTTTAT CCACACCAAT 
TGGCCAGAGC TTCTGCGTCT GCCCTTCCTC GAGGAGTTCA TTACACCAAT TGTGAAGGCA 
ACCAAGAAAA ACGAGGAGCT GTCATTCTAC TCGCTACCCG AATTCGAGGA GTGGAAAAAT 
GATACAGCTA ATCACCATAC GTACAATATA AAGTACTATA AGGGTTTGGG TACTTCGACC 
TCCAAGGAGG CGAAAGAGTA TTTTCAAGAC ATGGATCGCC ATCGCATCTT ATTTAAGTAC 
GATGGATCGG TGGATGATGA GAGCATTGTT ATGGCCTTCT CCAAGAAGCA CATCGAGTCG 
CGAAAGGTAT GGCTTACCAA CCACATGGAC GAGGTGAAGC GTCGCAAGGA GCTCGGTTTG 
CCAGAGCGAT ATCTCTACAC CAAGGGCACC AAGAGCATCA CCTATGCAGA CTTTATCAAT 
CTGGAGTTGG TGCTGTTCTC GAATGCTGAC AATGAGCGCT CCATTCCTAG TCTGGTGGAT 
GGCTTGAAGC CGGGTCAGCG GAAGGTGATG TTCACTTGCT TCAAGAGGAA TGACAAGCGT 
GAGGTGAAGG TGGCCCAGCT ATCCGGATCA GTGGCGGAGA TGTCAGCCTA TCACCACGGA 
GAGGTATCCC TACAGATGAC AATCGTGAAT CTGGCTCAGA ATTTTGTGGG TGCCAACAAC 
ATTAATCTGC TTGAGCCACG CGGTCAATTT GGTACCCGCT TGTCGGGAGG CAAAGATTGC 
GCCAGCGCTC GTTACATTTT CACTATAATG TCTCCCTTGA CGCGACTCAT CTACCATCCT 
TTGGACGATC CACTTTTGGA TTACCAGGTG GACGATGGCC AAAAGATCGA GCCACTATGG 
TATCTGCCCA TCATACCGAT GGTATTGGTA AACGGAGCCG AGGGCATAGG AACTGGATGG 
TCCACGAAGA TATCCAACTA CAATCCTCGT GAGATTATGA AAAATCTAAG GAAGATGATA 
AACGGACAAG AGCCAAGTGT GATGCATCCG TGGTACAAGA ACTTTTTAGG ACGCATGGAG 
TATGTTTCGG ATGGTCGTTA TATTCAGACT GGTAACATTC AAATTTTGTC CGGAAACCGT 
TTAGAAATCA GTGAACTCCC TGTGGGCGTA TGGACGCAAA ACTACAAGGA AAATGTCCTG 
GAGCCTTTAT CAAACGGCAC CGAAAAGGTT AAGGGTATTA TTTCCGAGTA CAGGGAGTAT 
CATACAGACA CCACCGTTCG CTTTGTGATC AGTTTCGCAC CTGGAGAATT TGAGCGCATT 
CATGCAGAAG AAGGTGGTTT CTACCGAGTG TTCAAACTTA CCACGACGCT GTCCACCAAC 
CAGATGCATG CGTTCGACCA GAACAACTGT CTGCGACGCT TCCCCACCGC GATCGATATC 
CTTAAAGAGT ATTACAAACT GCGACGAGAG TACTACGCCC GCCGGAGGGA CTTTTTGGTC 
GGCCAGCTCA CGGCGCAGGC TGATCGCCTC AGTGATCAGG CGCGCTTTAT TCTCGAAAAG 
TGTGAGAAGA AGCTGGTGGT GGAGAACAAG CAGCGCAAGG CCATGTGTGA TGAGTTGTTG 
AAGCGTGGAT ATCGTCCCGA TCCCGTCAAG GAGTGGCAGC GCCGCATTAA AATGGAGGAT 
GCCGAGCAAG CTGACGAAGA GGACGAAGAA GAAGAGGAGG CAGCTCCCAG TGTCAGCTCA 
AAGGCTAAGA AGGAAAAGGA AGTCGATCCG GAAAAGGCCT TTAAGAAACT AACTGATGTC 
AAAAAGTTTG ACTACCTTCT GGGTATGTCC ATGTGGATGT TGACAGAGGA GAAGAAAAAC 
GAGCTACTCA AACAGCGCGA CACTAAACTG TCCGAGTTGG AAAGTCTGCG CAAGAAGACG 
CCGGAGATGC TTTGGCTGGA TGATTTGGAT GCCCTCGAAT CGAAGTTAAA TGAAGTAGAG 
GAGAAGGAGC GCGCAGAAGA GCAGGGGATC AATCTCAAGA CCGCGAAGGC TTTGAAGGGC 
CAGAAGTCGG CCTCAGCTAA GGGCAGAAAG GTTAAGTCAA TGGGAGGCGG AGCGGGTGCC 
GGAGACGTAT TCCCAGATCC CGATGGAGAA CCCGTCGAGT TTAAGATCAC CGAAGAAATC 
ATTAAGAAAA TGGCAGCGGC TGCCAAGGTA GCTCAGGCGG CTAAGGAGCC GAAGAAACCC 
AAAGAGCCCA AGGAGCCAAA AGTGAAGAAG GAACCGAAGG GCAAGCAGAT TAAAGCCGAA 
CCTGATGCCA GCGGCGATGA GGTGGACGAA TTTGATGCAA TGGTCGAGGG CGGCTCTAAG 
ACCTCACCCA AGGCCAAGAA GGCGGTTGTC AAGAAGGAGC CGGGAGAAAA AAAGCCGCGC 
CAAAAGAAGG AGAACGGCGG CGGGCTCAAG CAGAGCAAGA TTGACTTCAG CAAGGCCAAG 
GCGAAAAAGA GCGACGATGA CGTGGAGGAA GTAACTCCTC GTGCAGAGCG TCCTGGACGT 
CGACAGGCCA GTAAGAAGAT AGACTACAGC TCGCTCTTCT CGGATGAGGA GGAAGACGGT 
GGCAACGTTG GCTCTGATGA TGATGGCAAT GCCAGCGATG ATGATTCGCC CAAGCGTCCA 
GCTAAGCGTG GACGAGAGGA TGAGAGCAGT GGAGGAGCCA AAAAGAAAGC TCCGCCCAAG 
AAGAGGCGAG CTGTAATTGA AAGCGATGAT GATGATATCG AGATTGATGA AGATGATGAT 
GATGATTCTG ATTTTAATTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Top2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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