Phlpp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phlpp
Entrez Gene ID	35178
Full Name	PH domain leucine-rich repeat protein phosphatase
Synonyms	CG10493,CT29458,Dmel\CG10493,PP2C,dPHLPP
General protein information	Preferred Names PH domain leucine-rich repeat protein phosphatase Names CG10493 gene product from transcript CG10493-RB CG10493-PA CG10493-PB Phlpp-PA Phlpp-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37B12-37B13

mRNA and Protein(s)

mRNA	Protein	Name
NM_136094.2	NP_609938.1	PH domain leucine-rich repeat protein phosphatase, isoform A
NM_001273629.1	NP_001260558.1	PH domain leucine-rich repeat protein phosphatase, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Phlpp	NP_609938.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009087	XP_319835.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Phlpp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phlpp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14934	NM_001273629.1 Latest version!	Drosophila melanogaster PH domain leucine-rich repeat protein phosphatase (Phlpp), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa14934	NM_136094.2 Latest version!	Drosophila melanogaster PH domain leucine-rich repeat protein phosphatase (Phlpp), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phlpp ( NM_136094.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609938.1 Drosophila melanogaster PH domain leucine-rich repeat protein phosphatase (Phlpp), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14934
Clone ID Related Accession (Same CDS sequence)	NM_136094.2 , NM_001273629.1
Accession Version	NM_136094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PH domain leucine-rich repeat protein phosphatase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_136094.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCAAAG CCACGAGGAA GCAAGCGGAT CCGTACAAAT CGAAGCTGAA AGTTAGTGCC 
AGCCACAGTG GACCGCATCC ACTGCCCGTC GAGGTGACGG CGGCAGAGGA GGAGCAGGCC 
GCCACTTTTG GCCAGACATC CCCCCAGAAG CTCAGCCTGA AGGGCAGCCA ACTGGGCGGC 
AGCATTTTGA TTGGCAACTA CAATTATTTG ACCCAGCTGG AGGTATGCGA AAACGAAATG 
GAGGTCTTGG ATCTCAGTTC GCTCGCTCAA CTGGAGACGC TGAAGTGCAG CCGGAACAAG 
CTGATGGAAC TGATCATAAA TGGCACCAAT TTGCAGACAC TTGTCGCCGA CCATAATTAC 
CTGCACAACA TATCCACAAC GAACACGCAT CCAGTTCCGC TGAAGTTGCA GCGCATTGAC 
ATCTCTCACA ATAACTTCAG TGAGCTCCCC AACTGGGTCG GAGCATGTGC CTCCTTGACC 
GCCATAAATG CTTCACACAA TCGCCTTAAC AACGTGGCAG TACTGTTACG CAACTACAGG 
ATAACTGAGC TGGTTTCCTT AGACCTGGCA TACAACGATC TGAAGCAACT GGACCAATTT 
CCGGAGGGCT TTTCAAGCAT CCGGAGTCTG CAGTTGCAAA GCAATGAGCT ACCGAGTCTT 
CCGGACAACT TTTTTGCCGT GACCCACGCT CGCCTGGAAA CGCTAAATGT CTCCTGCAAC 
AAACTGTCCA CCCTGCCACG CTACGAACAG AATAACCATG CTGCGCTGGT TAATTTATCC 
CTGGCGGGGA ATCACCTGAA TGATAGCATC TTTGAGCCCT TGCACAATGC CGCCAAGTTG 
AGAGTTCTCC ATCTGGCCTA CAATCGCATC GGGGTCCTGC CCGCCGCCTG TGTCCGCAAC 
TGGCCGGAAC TGGAGATCCT AGTTTTATCT GGTAATATGC TGCAACAACT GCCCGAGGAG 
GTGGCCACTT TAGGTCAGCT CAGGGTGCTA CGCTGCTGCA ACAATCTGCT CCTCTGCACG 
CCGCAACTTG CGAAGTTGGC CATGCTGAAG GTCCTTGATC TCTCGCACAA TCATCTGGAT 
CGCGTCAATC TGCTAGCATT GGTTCCATCA AGGAATCTAA AGTACCTGGA TCTATCGGGA 
AACTTGCAGT TGCAGGTGGA CGAGCAGCAG TTCAAGGTTT GTCAGAGCCA GAGCCAGCGC 
CATTGGAGCC TAGTCGATGT CTCCGGAAAC AATAGAGCTG CTTTGCCTAC TACCAAGATC 
AGACAGGTGA GTGCCCAACG GAATCAGAAC AAGACCAGTG GGCCTTGGAC CATGGGATTT 
GCGGAGACTC CTGGCTCTGG TGATTGCCGG AAGCTTTCAG TTTACCAACT AAGGGCAGCA 
AACTACGGGG GATCTGATGA GGCTCTGTAC GGGATGTTCG AGGCATTGGA GGGGCGCGGC 
CGGGCTGCCC AGGAGATGTC TCATTTGGTG CCCGATCTGA TGAAGCAGGA GCAGATGGTC 
AAGGACTCGG CGGTCAGGGA TTACATGAAG TTTACCCTGC TGGCGGCGCA GCAACAATGT 
GGTAGTGTGC GGAGTGCCGC CTTATTCCAT CTGACCAGGA CACGGGCTCC TTCCAAAGTA 
AGACCGCTGA AGAGCAAGCG ATACGTCCTA CGCATGGCAA GCACTGGAGG TCTGGACGCC 
TACCTGATAC GACGCACCTC CCAGTTGCGT CTTACCAAGC CGGATGTCAT TCAAAAGGAT 
CAGATTCATT CCATGCCAGA TCCACATGTG TTAGAGCTCA TTCTCAGCAA CGACGATGAG 
TACTTGGTGG TGGGCAATGC TCAGCTCTGG TCAGTGATGG ATATTGATCG TGCAGCCCGA 
GAGATCCGAA AGGAGGAGAA TTCCCTGCTT GCGGCCAAGC GACTGGTGGA CATTGCTCAA 
AGCTTTGCTG CGGCGGAGAG TCTCAGTGTA ATTGTGGTTC GCTTTCGCCA CCTCGGTACG 
GATGTGGACC ACCTGATACG AGAGCTTAAA CAAAGTGTGC GCAAGAAGCC CCAGCCGGTT 
TCCTTACCTT TGTCCAGTGG ATCAGTTTGC AAGCGAACGT GCTGCGACCG GAGCAATGCC 
TGTCGCCACC GAGCCATTGA ACAGGAGCCG CTGGCAGGAC GATCCTCGCC AAGTGGGCAA 
AGTGATCGGG ACTTGCTGGC CAAGGATAAG GACGACGAGT TCGTACTAGC CCATGCCCGT 
GTCCTGCAGG AGGAGCAACA GCTTGAGATG CTGGACGAAA CAGAGTCGGT ATCAGAATCA 
GTGCTTTCTG AGGAGCAGTT CAAGTGCTGG GAGTACATGC TGGAGCAGAA CACCCAGCTG 
CTGTTCGATA AGGAGCTAAA CACCATCTCT AAGTCTTTTA CTAAACAACG TACCGTACCC 
AATGCTATCA TGGCAGCTAC AGTTCTCCCA GAACGCAATG ACTTTACCTC GAATTTGATG 
CGGACTGTCA CCAACAAGTT CATCTCGACC AGCACTCCTC AATTACCCCA ACCCATAACG 
ACTTCTGTGC CCCTGGGTTC ATATCATCAG GTGAAGCAAG CGCCTCCTGG ACACTTTGGC 
AGTGCCCTCT CCTTCCAGCA GGCCCACAGC TACGGCTATA ATCTGTTTGA TGCCAAGCCA 
AGGCCCAAGT TTCACGGAGG CACAGTCAAG CGATCCGCAG GACCAAATTC AGCCTACTTT 
GGATCCCTTC AGCGCCTGAT GCCCTACAAT TTCGAGTACG ACTTTGCCGT TACCCAGGAG 
AGGGAGAGAA ACATCCTGGA CGAAGAGGAG CACGACGATG ATGACTTCAA CGAGCACGAG 
AGTAGAATGC GAAAATACTG GGGAGTGGCC ACGACGGAAC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609938.1
CDS	334..3198
Translation	MLKATRKQADPYKSKLKVSASHSGPHPLPVEVTAAEEEQAATFGQTSPQKLSLKGSQLGGSILIGNYNYLTQLEVCENEMEVLDLSSLAQLETLKCSRNKLMELIINGTNLQTLVADHNYLHNISTTNTHPVPLKLQRIDISHNNFSELPNWVGACASLTAINASHNRLNNVAVLLRNYRITELVSLDLAYNDLKQLDQFPEGFSSIRSLQLQSNELPSLPDNFFAVTHARLETLNVSCNKLSTLPRYEQNNHAALVNLSLAGNHLNDSIFEPLHNAAKLRVLHLAYNRIGVLPAACVRNWPELEILVLSGNMLQQLPEEVATLGQLRVLRCCNNLLLCTPQLAKLAMLKVLDLSHNHLDRVNLLALVPSRNLKYLDLSGNLQLQVDEQQFKVCQSQSQRHWSLVDVSGNNRAALPTTKIRQVSAQRNQNKTSGPWTMGFAETPGSGDCRKLSVYQLRAANYGGSDEALYGMFEALEGRGRAAQEMSHLVPDLMKQEQMVKDSAVRDYMKFTLLAAQQQCGSVRSAALFHLTRTRAPSKVRPLKSKRYVLRMASTGGLDAYLIRRTSQLRLTKPDVIQKDQIHSMPDPHVLELILSNDDEYLVVGNAQLWSVMDIDRAAREIRKEENSLLAAKRLVDIAQSFAAAESLSVIVVRFRHLGTDVDHLIRELKQSVRKKPQPVSLPLSSGSVCKRTCCDRSNACRHRAIEQEPLAGRSSPSGQSDRDLLAKDKDDEFVLAHARVLQEEQQLEMLDETESVSESVLSEEQFKCWEYMLEQNTQLLFDKELNTISKSFTKQRTVPNAIMAATVLPERNDFTSNLMRTVTNKFISTSTPQLPQPITTSVPLGSYHQVKQAPPGHFGSALSFQQAHSYGYNLFDAKPRPKFHGGTVKRSAGPNSAYFGSLQRLMPYNFEYDFAVTQERERNILDEEEHDDDDFNEHESRMRKYWGVATTEL

Target ORF information:

RefSeq Version	NM_136094.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PH domain leucine-rich repeat protein phosphatase (Phlpp), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136094.2

ORF Insert Sequence:

ATGCTCAAAG CCACGAGGAA GCAAGCGGAT CCGTACAAAT CGAAGCTGAA AGTTAGTGCC 
AGCCACAGTG GACCGCATCC ACTGCCCGTC GAGGTGACGG CGGCAGAGGA GGAGCAGGCC 
GCCACTTTTG GCCAGACATC CCCCCAGAAG CTCAGCCTGA AGGGCAGCCA ACTGGGCGGC 
AGCATTTTGA TTGGCAACTA CAATTATTTG ACCCAGCTGG AGGTATGCGA AAACGAAATG 
GAGGTCTTGG ATCTCAGTTC GCTCGCTCAA CTGGAGACGC TGAAGTGCAG CCGGAACAAG 
CTGATGGAAC TGATCATAAA TGGCACCAAT TTGCAGACAC TTGTCGCCGA CCATAATTAC 
CTGCACAACA TATCCACAAC GAACACGCAT CCAGTTCCGC TGAAGTTGCA GCGCATTGAC 
ATCTCTCACA ATAACTTCAG TGAGCTCCCC AACTGGGTCG GAGCATGTGC CTCCTTGACC 
GCCATAAATG CTTCACACAA TCGCCTTAAC AACGTGGCAG TACTGTTACG CAACTACAGG 
ATAACTGAGC TGGTTTCCTT AGACCTGGCA TACAACGATC TGAAGCAACT GGACCAATTT 
CCGGAGGGCT TTTCAAGCAT CCGGAGTCTG CAGTTGCAAA GCAATGAGCT ACCGAGTCTT 
CCGGACAACT TTTTTGCCGT GACCCACGCT CGCCTGGAAA CGCTAAATGT CTCCTGCAAC 
AAACTGTCCA CCCTGCCACG CTACGAACAG AATAACCATG CTGCGCTGGT TAATTTATCC 
CTGGCGGGGA ATCACCTGAA TGATAGCATC TTTGAGCCCT TGCACAATGC CGCCAAGTTG 
AGAGTTCTCC ATCTGGCCTA CAATCGCATC GGGGTCCTGC CCGCCGCCTG TGTCCGCAAC 
TGGCCGGAAC TGGAGATCCT AGTTTTATCT GGTAATATGC TGCAACAACT GCCCGAGGAG 
GTGGCCACTT TAGGTCAGCT CAGGGTGCTA CGCTGCTGCA ACAATCTGCT CCTCTGCACG 
CCGCAACTTG CGAAGTTGGC CATGCTGAAG GTCCTTGATC TCTCGCACAA TCATCTGGAT 
CGCGTCAATC TGCTAGCATT GGTTCCATCA AGGAATCTAA AGTACCTGGA TCTATCGGGA 
AACTTGCAGT TGCAGGTGGA CGAGCAGCAG TTCAAGGTTT GTCAGAGCCA GAGCCAGCGC 
CATTGGAGCC TAGTCGATGT CTCCGGAAAC AATAGAGCTG CTTTGCCTAC TACCAAGATC 
AGACAGGTGA GTGCCCAACG GAATCAGAAC AAGACCAGTG GGCCTTGGAC CATGGGATTT 
GCGGAGACTC CTGGCTCTGG TGATTGCCGG AAGCTTTCAG TTTACCAACT AAGGGCAGCA 
AACTACGGGG GATCTGATGA GGCTCTGTAC GGGATGTTCG AGGCATTGGA GGGGCGCGGC 
CGGGCTGCCC AGGAGATGTC TCATTTGGTG CCCGATCTGA TGAAGCAGGA GCAGATGGTC 
AAGGACTCGG CGGTCAGGGA TTACATGAAG TTTACCCTGC TGGCGGCGCA GCAACAATGT 
GGTAGTGTGC GGAGTGCCGC CTTATTCCAT CTGACCAGGA CACGGGCTCC TTCCAAAGTA 
AGACCGCTGA AGAGCAAGCG ATACGTCCTA CGCATGGCAA GCACTGGAGG TCTGGACGCC 
TACCTGATAC GACGCACCTC CCAGTTGCGT CTTACCAAGC CGGATGTCAT TCAAAAGGAT 
CAGATTCATT CCATGCCAGA TCCACATGTG TTAGAGCTCA TTCTCAGCAA CGACGATGAG 
TACTTGGTGG TGGGCAATGC TCAGCTCTGG TCAGTGATGG ATATTGATCG TGCAGCCCGA 
GAGATCCGAA AGGAGGAGAA TTCCCTGCTT GCGGCCAAGC GACTGGTGGA CATTGCTCAA 
AGCTTTGCTG CGGCGGAGAG TCTCAGTGTA ATTGTGGTTC GCTTTCGCCA CCTCGGTACG 
GATGTGGACC ACCTGATACG AGAGCTTAAA CAAAGTGTGC GCAAGAAGCC CCAGCCGGTT 
TCCTTACCTT TGTCCAGTGG ATCAGTTTGC AAGCGAACGT GCTGCGACCG GAGCAATGCC 
TGTCGCCACC GAGCCATTGA ACAGGAGCCG CTGGCAGGAC GATCCTCGCC AAGTGGGCAA 
AGTGATCGGG ACTTGCTGGC CAAGGATAAG GACGACGAGT TCGTACTAGC CCATGCCCGT 
GTCCTGCAGG AGGAGCAACA GCTTGAGATG CTGGACGAAA CAGAGTCGGT ATCAGAATCA 
GTGCTTTCTG AGGAGCAGTT CAAGTGCTGG GAGTACATGC TGGAGCAGAA CACCCAGCTG 
CTGTTCGATA AGGAGCTAAA CACCATCTCT AAGTCTTTTA CTAAACAACG TACCGTACCC 
AATGCTATCA TGGCAGCTAC AGTTCTCCCA GAACGCAATG ACTTTACCTC GAATTTGATG 
CGGACTGTCA CCAACAAGTT CATCTCGACC AGCACTCCTC AATTACCCCA ACCCATAACG 
ACTTCTGTGC CCCTGGGTTC ATATCATCAG GTGAAGCAAG CGCCTCCTGG ACACTTTGGC 
AGTGCCCTCT CCTTCCAGCA GGCCCACAGC TACGGCTATA ATCTGTTTGA TGCCAAGCCA 
AGGCCCAAGT TTCACGGAGG CACAGTCAAG CGATCCGCAG GACCAAATTC AGCCTACTTT 
GGATCCCTTC AGCGCCTGAT GCCCTACAAT TTCGAGTACG ACTTTGCCGT TACCCAGGAG 
AGGGAGAGAA ACATCCTGGA CGAAGAGGAG CACGACGATG ATGACTTCAA CGAGCACGAG 
AGTAGAATGC GAAAATACTG GGGAGTGGCC ACGACGGAAC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phlpp ( NM_001273629.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260558.1 Drosophila melanogaster PH domain leucine-rich repeat protein phosphatase (Phlpp), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14934
Clone ID Related Accession (Same CDS sequence)	NM_136094.2 , NM_001273629.1
Accession Version	NM_001273629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PH domain leucine-rich repeat protein phosphatase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCAAAG CCACGAGGAA GCAAGCGGAT CCGTACAAAT CGAAGCTGAA AGTTAGTGCC 
AGCCACAGTG GACCGCATCC ACTGCCCGTC GAGGTGACGG CGGCAGAGGA GGAGCAGGCC 
GCCACTTTTG GCCAGACATC CCCCCAGAAG CTCAGCCTGA AGGGCAGCCA ACTGGGCGGC 
AGCATTTTGA TTGGCAACTA CAATTATTTG ACCCAGCTGG AGGTATGCGA AAACGAAATG 
GAGGTCTTGG ATCTCAGTTC GCTCGCTCAA CTGGAGACGC TGAAGTGCAG CCGGAACAAG 
CTGATGGAAC TGATCATAAA TGGCACCAAT TTGCAGACAC TTGTCGCCGA CCATAATTAC 
CTGCACAACA TATCCACAAC GAACACGCAT CCAGTTCCGC TGAAGTTGCA GCGCATTGAC 
ATCTCTCACA ATAACTTCAG TGAGCTCCCC AACTGGGTCG GAGCATGTGC CTCCTTGACC 
GCCATAAATG CTTCACACAA TCGCCTTAAC AACGTGGCAG TACTGTTACG CAACTACAGG 
ATAACTGAGC TGGTTTCCTT AGACCTGGCA TACAACGATC TGAAGCAACT GGACCAATTT 
CCGGAGGGCT TTTCAAGCAT CCGGAGTCTG CAGTTGCAAA GCAATGAGCT ACCGAGTCTT 
CCGGACAACT TTTTTGCCGT GACCCACGCT CGCCTGGAAA CGCTAAATGT CTCCTGCAAC 
AAACTGTCCA CCCTGCCACG CTACGAACAG AATAACCATG CTGCGCTGGT TAATTTATCC 
CTGGCGGGGA ATCACCTGAA TGATAGCATC TTTGAGCCCT TGCACAATGC CGCCAAGTTG 
AGAGTTCTCC ATCTGGCCTA CAATCGCATC GGGGTCCTGC CCGCCGCCTG TGTCCGCAAC 
TGGCCGGAAC TGGAGATCCT AGTTTTATCT GGTAATATGC TGCAACAACT GCCCGAGGAG 
GTGGCCACTT TAGGTCAGCT CAGGGTGCTA CGCTGCTGCA ACAATCTGCT CCTCTGCACG 
CCGCAACTTG CGAAGTTGGC CATGCTGAAG GTCCTTGATC TCTCGCACAA TCATCTGGAT 
CGCGTCAATC TGCTAGCATT GGTTCCATCA AGGAATCTAA AGTACCTGGA TCTATCGGGA 
AACTTGCAGT TGCAGGTGGA CGAGCAGCAG TTCAAGGTTT GTCAGAGCCA GAGCCAGCGC 
CATTGGAGCC TAGTCGATGT CTCCGGAAAC AATAGAGCTG CTTTGCCTAC TACCAAGATC 
AGACAGGTGA GTGCCCAACG GAATCAGAAC AAGACCAGTG GGCCTTGGAC CATGGGATTT 
GCGGAGACTC CTGGCTCTGG TGATTGCCGG AAGCTTTCAG TTTACCAACT AAGGGCAGCA 
AACTACGGGG GATCTGATGA GGCTCTGTAC GGGATGTTCG AGGCATTGGA GGGGCGCGGC 
CGGGCTGCCC AGGAGATGTC TCATTTGGTG CCCGATCTGA TGAAGCAGGA GCAGATGGTC 
AAGGACTCGG CGGTCAGGGA TTACATGAAG TTTACCCTGC TGGCGGCGCA GCAACAATGT 
GGTAGTGTGC GGAGTGCCGC CTTATTCCAT CTGACCAGGA CACGGGCTCC TTCCAAAGTA 
AGACCGCTGA AGAGCAAGCG ATACGTCCTA CGCATGGCAA GCACTGGAGG TCTGGACGCC 
TACCTGATAC GACGCACCTC CCAGTTGCGT CTTACCAAGC CGGATGTCAT TCAAAAGGAT 
CAGATTCATT CCATGCCAGA TCCACATGTG TTAGAGCTCA TTCTCAGCAA CGACGATGAG 
TACTTGGTGG TGGGCAATGC TCAGCTCTGG TCAGTGATGG ATATTGATCG TGCAGCCCGA 
GAGATCCGAA AGGAGGAGAA TTCCCTGCTT GCGGCCAAGC GACTGGTGGA CATTGCTCAA 
AGCTTTGCTG CGGCGGAGAG TCTCAGTGTA ATTGTGGTTC GCTTTCGCCA CCTCGGTACG 
GATGTGGACC ACCTGATACG AGAGCTTAAA CAAAGTGTGC GCAAGAAGCC CCAGCCGGTT 
TCCTTACCTT TGTCCAGTGG ATCAGTTTGC AAGCGAACGT GCTGCGACCG GAGCAATGCC 
TGTCGCCACC GAGCCATTGA ACAGGAGCCG CTGGCAGGAC GATCCTCGCC AAGTGGGCAA 
AGTGATCGGG ACTTGCTGGC CAAGGATAAG GACGACGAGT TCGTACTAGC CCATGCCCGT 
GTCCTGCAGG AGGAGCAACA GCTTGAGATG CTGGACGAAA CAGAGTCGGT ATCAGAATCA 
GTGCTTTCTG AGGAGCAGTT CAAGTGCTGG GAGTACATGC TGGAGCAGAA CACCCAGCTG 
CTGTTCGATA AGGAGCTAAA CACCATCTCT AAGTCTTTTA CTAAACAACG TACCGTACCC 
AATGCTATCA TGGCAGCTAC AGTTCTCCCA GAACGCAATG ACTTTACCTC GAATTTGATG 
CGGACTGTCA CCAACAAGTT CATCTCGACC AGCACTCCTC AATTACCCCA ACCCATAACG 
ACTTCTGTGC CCCTGGGTTC ATATCATCAG GTGAAGCAAG CGCCTCCTGG ACACTTTGGC 
AGTGCCCTCT CCTTCCAGCA GGCCCACAGC TACGGCTATA ATCTGTTTGA TGCCAAGCCA 
AGGCCCAAGT TTCACGGAGG CACAGTCAAG CGATCCGCAG GACCAAATTC AGCCTACTTT 
GGATCCCTTC AGCGCCTGAT GCCCTACAAT TTCGAGTACG ACTTTGCCGT TACCCAGGAG 
AGGGAGAGAA ACATCCTGGA CGAAGAGGAG CACGACGATG ATGACTTCAA CGAGCACGAG 
AGTAGAATGC GAAAATACTG GGGAGTGGCC ACGACGGAAC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260558.1
CDS	519..3383
Translation	MLKATRKQADPYKSKLKVSASHSGPHPLPVEVTAAEEEQAATFGQTSPQKLSLKGSQLGGSILIGNYNYLTQLEVCENEMEVLDLSSLAQLETLKCSRNKLMELIINGTNLQTLVADHNYLHNISTTNTHPVPLKLQRIDISHNNFSELPNWVGACASLTAINASHNRLNNVAVLLRNYRITELVSLDLAYNDLKQLDQFPEGFSSIRSLQLQSNELPSLPDNFFAVTHARLETLNVSCNKLSTLPRYEQNNHAALVNLSLAGNHLNDSIFEPLHNAAKLRVLHLAYNRIGVLPAACVRNWPELEILVLSGNMLQQLPEEVATLGQLRVLRCCNNLLLCTPQLAKLAMLKVLDLSHNHLDRVNLLALVPSRNLKYLDLSGNLQLQVDEQQFKVCQSQSQRHWSLVDVSGNNRAALPTTKIRQVSAQRNQNKTSGPWTMGFAETPGSGDCRKLSVYQLRAANYGGSDEALYGMFEALEGRGRAAQEMSHLVPDLMKQEQMVKDSAVRDYMKFTLLAAQQQCGSVRSAALFHLTRTRAPSKVRPLKSKRYVLRMASTGGLDAYLIRRTSQLRLTKPDVIQKDQIHSMPDPHVLELILSNDDEYLVVGNAQLWSVMDIDRAAREIRKEENSLLAAKRLVDIAQSFAAAESLSVIVVRFRHLGTDVDHLIRELKQSVRKKPQPVSLPLSSGSVCKRTCCDRSNACRHRAIEQEPLAGRSSPSGQSDRDLLAKDKDDEFVLAHARVLQEEQQLEMLDETESVSESVLSEEQFKCWEYMLEQNTQLLFDKELNTISKSFTKQRTVPNAIMAATVLPERNDFTSNLMRTVTNKFISTSTPQLPQPITTSVPLGSYHQVKQAPPGHFGSALSFQQAHSYGYNLFDAKPRPKFHGGTVKRSAGPNSAYFGSLQRLMPYNFEYDFAVTQERERNILDEEEHDDDDFNEHESRMRKYWGVATTEL

Target ORF information:

RefSeq Version	NM_001273629.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PH domain leucine-rich repeat protein phosphatase (Phlpp), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273629.1

ORF Insert Sequence:

ATGCTCAAAG CCACGAGGAA GCAAGCGGAT CCGTACAAAT CGAAGCTGAA AGTTAGTGCC 
AGCCACAGTG GACCGCATCC ACTGCCCGTC GAGGTGACGG CGGCAGAGGA GGAGCAGGCC 
GCCACTTTTG GCCAGACATC CCCCCAGAAG CTCAGCCTGA AGGGCAGCCA ACTGGGCGGC 
AGCATTTTGA TTGGCAACTA CAATTATTTG ACCCAGCTGG AGGTATGCGA AAACGAAATG 
GAGGTCTTGG ATCTCAGTTC GCTCGCTCAA CTGGAGACGC TGAAGTGCAG CCGGAACAAG 
CTGATGGAAC TGATCATAAA TGGCACCAAT TTGCAGACAC TTGTCGCCGA CCATAATTAC 
CTGCACAACA TATCCACAAC GAACACGCAT CCAGTTCCGC TGAAGTTGCA GCGCATTGAC 
ATCTCTCACA ATAACTTCAG TGAGCTCCCC AACTGGGTCG GAGCATGTGC CTCCTTGACC 
GCCATAAATG CTTCACACAA TCGCCTTAAC AACGTGGCAG TACTGTTACG CAACTACAGG 
ATAACTGAGC TGGTTTCCTT AGACCTGGCA TACAACGATC TGAAGCAACT GGACCAATTT 
CCGGAGGGCT TTTCAAGCAT CCGGAGTCTG CAGTTGCAAA GCAATGAGCT ACCGAGTCTT 
CCGGACAACT TTTTTGCCGT GACCCACGCT CGCCTGGAAA CGCTAAATGT CTCCTGCAAC 
AAACTGTCCA CCCTGCCACG CTACGAACAG AATAACCATG CTGCGCTGGT TAATTTATCC 
CTGGCGGGGA ATCACCTGAA TGATAGCATC TTTGAGCCCT TGCACAATGC CGCCAAGTTG 
AGAGTTCTCC ATCTGGCCTA CAATCGCATC GGGGTCCTGC CCGCCGCCTG TGTCCGCAAC 
TGGCCGGAAC TGGAGATCCT AGTTTTATCT GGTAATATGC TGCAACAACT GCCCGAGGAG 
GTGGCCACTT TAGGTCAGCT CAGGGTGCTA CGCTGCTGCA ACAATCTGCT CCTCTGCACG 
CCGCAACTTG CGAAGTTGGC CATGCTGAAG GTCCTTGATC TCTCGCACAA TCATCTGGAT 
CGCGTCAATC TGCTAGCATT GGTTCCATCA AGGAATCTAA AGTACCTGGA TCTATCGGGA 
AACTTGCAGT TGCAGGTGGA CGAGCAGCAG TTCAAGGTTT GTCAGAGCCA GAGCCAGCGC 
CATTGGAGCC TAGTCGATGT CTCCGGAAAC AATAGAGCTG CTTTGCCTAC TACCAAGATC 
AGACAGGTGA GTGCCCAACG GAATCAGAAC AAGACCAGTG GGCCTTGGAC CATGGGATTT 
GCGGAGACTC CTGGCTCTGG TGATTGCCGG AAGCTTTCAG TTTACCAACT AAGGGCAGCA 
AACTACGGGG GATCTGATGA GGCTCTGTAC GGGATGTTCG AGGCATTGGA GGGGCGCGGC 
CGGGCTGCCC AGGAGATGTC TCATTTGGTG CCCGATCTGA TGAAGCAGGA GCAGATGGTC 
AAGGACTCGG CGGTCAGGGA TTACATGAAG TTTACCCTGC TGGCGGCGCA GCAACAATGT 
GGTAGTGTGC GGAGTGCCGC CTTATTCCAT CTGACCAGGA CACGGGCTCC TTCCAAAGTA 
AGACCGCTGA AGAGCAAGCG ATACGTCCTA CGCATGGCAA GCACTGGAGG TCTGGACGCC 
TACCTGATAC GACGCACCTC CCAGTTGCGT CTTACCAAGC CGGATGTCAT TCAAAAGGAT 
CAGATTCATT CCATGCCAGA TCCACATGTG TTAGAGCTCA TTCTCAGCAA CGACGATGAG 
TACTTGGTGG TGGGCAATGC TCAGCTCTGG TCAGTGATGG ATATTGATCG TGCAGCCCGA 
GAGATCCGAA AGGAGGAGAA TTCCCTGCTT GCGGCCAAGC GACTGGTGGA CATTGCTCAA 
AGCTTTGCTG CGGCGGAGAG TCTCAGTGTA ATTGTGGTTC GCTTTCGCCA CCTCGGTACG 
GATGTGGACC ACCTGATACG AGAGCTTAAA CAAAGTGTGC GCAAGAAGCC CCAGCCGGTT 
TCCTTACCTT TGTCCAGTGG ATCAGTTTGC AAGCGAACGT GCTGCGACCG GAGCAATGCC 
TGTCGCCACC GAGCCATTGA ACAGGAGCCG CTGGCAGGAC GATCCTCGCC AAGTGGGCAA 
AGTGATCGGG ACTTGCTGGC CAAGGATAAG GACGACGAGT TCGTACTAGC CCATGCCCGT 
GTCCTGCAGG AGGAGCAACA GCTTGAGATG CTGGACGAAA CAGAGTCGGT ATCAGAATCA 
GTGCTTTCTG AGGAGCAGTT CAAGTGCTGG GAGTACATGC TGGAGCAGAA CACCCAGCTG 
CTGTTCGATA AGGAGCTAAA CACCATCTCT AAGTCTTTTA CTAAACAACG TACCGTACCC 
AATGCTATCA TGGCAGCTAC AGTTCTCCCA GAACGCAATG ACTTTACCTC GAATTTGATG 
CGGACTGTCA CCAACAAGTT CATCTCGACC AGCACTCCTC AATTACCCCA ACCCATAACG 
ACTTCTGTGC CCCTGGGTTC ATATCATCAG GTGAAGCAAG CGCCTCCTGG ACACTTTGGC 
AGTGCCCTCT CCTTCCAGCA GGCCCACAGC TACGGCTATA ATCTGTTTGA TGCCAAGCCA 
AGGCCCAAGT TTCACGGAGG CACAGTCAAG CGATCCGCAG GACCAAATTC AGCCTACTTT 
GGATCCCTTC AGCGCCTGAT GCCCTACAAT TTCGAGTACG ACTTTGCCGT TACCCAGGAG 
AGGGAGAGAA ACATCCTGGA CGAAGAGGAG CACGACGATG ATGACTTCAA CGAGCACGAG 
AGTAGAATGC GAAAATACTG GGGAGTGGCC ACGACGGAAC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Phlpp cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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