hook cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hook
Entrez Gene ID	35169
Full Name	CG10653 gene product from transcript CG10653-RA
Synonyms	CG10653,Dmel\CG10653,Hook,dHk,hk
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37B10-37B10\|2-53.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057225.4	NP_476573.1	hook

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Hook3	NP_997542.1
Drosophila melanogaster (fruit fly)	hk	NP_476573.1
Pan troglodytes (chimpanzee)	HOOK3	XP_001145260.1
Gallus gallus (chicken)	HOOK3	XP_003643087.2
	HOOK3	XP_001099564.2
Anopheles gambiae (African malaria mosquito)	HOOK_ANOGA	XP_319596.4
Xenopus tropicalis (tropical clawed frog)	hook3	XP_004910960.1
Homo sapiens (human)	HOOK3	NP_115786.1
Canis lupus familiaris (dog)	HOOK3	XP_005629843.1
Bos taurus (cattle)	HOOK3	NP_001179529.1
Rattus norvegicus (Norway rat)	Hook3	NP_001129570.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rab11 facilitates cross-talk between autophagy and endosomal pathway through regulation of Hook localization.
Title: Rab11 facilitates cross-talk between autophagy and endosomal pathway through regulation of Hook localization.

The following hook gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hook cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16036	NM_057225.4 Latest version!	Drosophila melanogaster hook (hook), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hook ( NM_057225.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476573.1 Drosophila melanogaster hook (hook), mRNA.

Price & Availability↑

CloneID	OFa16036
Clone ID Related Accession (Same CDS sequence)	NM_057225.4
Accession Version	NM_057225.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hook
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057225.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGCGC CCAAGAACGA GATGTACTAT AGCTTGCTGG AGTGGTTCAA GACCCTCAAT 
CTAAATGCCC CGCATGCGGA TGCGGAATCC TTAGCTGACG GAGTGGCACT GGCTCAGGCT 
CTTAACCAGT TCGCCCCCGA ATCCTTCACC GACGCTTGGC TTTCCAAGAT CAAGGCCAGT 
GCCGTGGGCA GCAACTGGCG ATTGCGGATG AGCAATCTTA AAAAGGTGAC CCAGTCCGTG 
TACGACTACT ACAGCGACGT ACTGAACTAC TCGCTGAGTG ATTTCTCGAA GCCTGATCTG 
CAGAGGATCG CTGAGAAGTG CGACTTGGGA GAGCTGGAGC GTCTGCTGCA GTTGGTCCTT 
GGTTGCGCCG TGAACTGTGC AGAGAAGCAG TCCTACATCA CAGAGATCAT GTGTCTGGAG 
GAGGAACTGC AAGCAAACAT TATGCGCGCC CTTCAGGAAC TTGAGGCCAC TAGGCAAGCA 
TCCACGCCGG AGGGCGGTGT AGCCAGTTCC TTGTCCCGTG GCTCTCGCAC AGGACTCCTG 
GACAGCAAGG CAGTTCAGGA GGATCGCGAC GCCCTGGCAC AAAAATGCTT CGAGACGGAA 
AAGAAAATGC TCCTTCTCAT AGACGAGAAA ACGAATCTCC AACAGGAGTT GCATAAGCTG 
CAGCAAGAGT TTGCCCGCCT AGAGCAACAC TCCACGGTGA TCGGAGATGA TGGAGTCTCT 
TTGGGGCCAG TTCAAACAGG ATCTGTGCGC TACAACGAAC TCCGGCGTCA ATTGGACCTA 
CTAAAAGAGG AACTTCTTCA GTCTGAAGGA GCTCGGGAGG ACCTTAAAAT CAAGGCCCAG 
CAACAAGATA CCGATCTGCT GCACATGCAA ATGCGCATAG AGGAATTAAT GAAAAGTTCC 
GCTGAAGTGA CCACACTGAA GGATGAAGTG GATGTTCTGC GGGAGTCCAA CGACAAGCTT 
AAGATATGCG AGGCCCAGCT TGACACGTAT AAAAAGAAGC TAGAAGACTA TAACGACCTG 
AAAAAACAAG TAAAAATTCT GGAGGAGCGT AGCGCCGACT ATGTGCAGCA GAATGCGCAG 
TTTGAGGAGG ATGCCAAACG ATATGCCAAC ACCAAAGGCC AAGTGGAGCT GTTCAAGAAA 
GAGATTCAAG ACCTTCATGC CAAGCTGGAC GCCGAGAGCA GCAAGAATGT AAAGTTGGAG 
TTTGATAACA AGAATTTGGA AAGCAAGAAC CTGGCTCTGC AGCGGGCCAA AGACAGTTTG 
CTCAAGGAGC GCGACAACCT TCGCGAGGCA GTCGACGAGC TGAAATGCGG ACAATTGTCC 
TCGAACACAG CGCTGACAGG CACCACGGTT TCCAGGGAAC TGCAACCGTC GGCCACAGTG 
GAAAAACTAC AGCGACTGGA GGCGGAGAAC AAGGCTCTGC GCGAGGGACA GGGTGGCCAG 
ACAGCTTTGG CGCAACTGCT GGACGATGCC AATAAACGCT GCGAGAATTT GCGTGAGCAG 
CTAAAAACAG CCAATGAACG CATTCTTTCC CTGTCGCATG CTTCTCAAAG CGATGATCCC 
ATTTTAAAAG AGAGCGAGTT TGGTAAACAA ATCAAACAGT TAATGGAGCT AAATGAACAA 
AAGACACTCC AACTGGAGGA AGCAGTTACC CAGAGCACGT CTCTGCAATG CAAGGTGACT 
CAGCTGGAAA CCAATTTATC AGCGCGTGAG CAGGAAATTC TGGTGTATGA CGCCAAATAC 
CGAAAATGCG TGGAGAAAGC TAAGGAGGTG ATCAAAAGCA TAGATCCTCG AATTGCCAGT 
GCTCTGGATG CTAGCGTACT GGAAAAGAGT GCTGATCTCG TCGAGGAGGA GCCCAAGCCG 
AAGATGAGTG TGATGGAGGA GCAACTCATG ACCTCCGCTT TCTACAGACT GGGTGTCAAT 
GCCCAGCGTG ATGCAATCGA CTCTAAGCTG GCAATATTGA TGGGCTCTGG CCAGACATTC 
CTTGCCCGCC AGCGGCAGTC CGCGCCCCGC AAATCCCTAA GTGCAATGAA ATCAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476573.1
CDS	119..2158
Translation	MSAPKNEMYYSLLEWFKTLNLNAPHADAESLADGVALAQALNQFAPESFTDAWLSKIKASAVGSNWRLRMSNLKKVTQSVYDYYSDVLNYSLSDFSKPDLQRIAEKCDLGELERLLQLVLGCAVNCAEKQSYITEIMCLEEELQANIMRALQELEATRQASTPEGGVASSLSRGSRTGLLDSKAVQEDRDALAQKCFETEKKMLLLIDEKTNLQQELHKLQQEFARLEQHSTVIGDDGVSLGPVQTGSVRYNELRRQLDLLKEELLQSEGAREDLKIKAQQQDTDLLHMQMRIEELMKSSAEVTTLKDEVDVLRESNDKLKICEAQLDTYKKKLEDYNDLKKQVKILEERSADYVQQNAQFEEDAKRYANTKGQVELFKKEIQDLHAKLDAESSKNVKLEFDNKNLESKNLALQRAKDSLLKERDNLREAVDELKCGQLSSNTALTGTTVSRELQPSATVEKLQRLEAENKALREGQGGQTALAQLLDDANKRCENLREQLKTANERILSLSHASQSDDPILKESEFGKQIKQLMELNEQKTLQLEEAVTQSTSLQCKVTQLETNLSAREQEILVYDAKYRKCVEKAKEVIKSIDPRIASALDASVLEKSADLVEEEPKPKMSVMEEQLMTSAFYRLGVNAQRDAIDSKLAILMGSGQTFLARQRQSAPRKSLSAMKSK

Target ORF information:

RefSeq Version	NM_057225.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hook (hook), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057225.4

ORF Insert Sequence:

ATGTCCGCGC CCAAGAACGA GATGTACTAT AGCTTGCTGG AGTGGTTCAA GACCCTCAAT 
CTAAATGCCC CGCATGCGGA TGCGGAATCC TTAGCTGACG GAGTGGCACT GGCTCAGGCT 
CTTAACCAGT TCGCCCCCGA ATCCTTCACC GACGCTTGGC TTTCCAAGAT CAAGGCCAGT 
GCCGTGGGCA GCAACTGGCG ATTGCGGATG AGCAATCTTA AAAAGGTGAC CCAGTCCGTG 
TACGACTACT ACAGCGACGT ACTGAACTAC TCGCTGAGTG ATTTCTCGAA GCCTGATCTG 
CAGAGGATCG CTGAGAAGTG CGACTTGGGA GAGCTGGAGC GTCTGCTGCA GTTGGTCCTT 
GGTTGCGCCG TGAACTGTGC AGAGAAGCAG TCCTACATCA CAGAGATCAT GTGTCTGGAG 
GAGGAACTGC AAGCAAACAT TATGCGCGCC CTTCAGGAAC TTGAGGCCAC TAGGCAAGCA 
TCCACGCCGG AGGGCGGTGT AGCCAGTTCC TTGTCCCGTG GCTCTCGCAC AGGACTCCTG 
GACAGCAAGG CAGTTCAGGA GGATCGCGAC GCCCTGGCAC AAAAATGCTT CGAGACGGAA 
AAGAAAATGC TCCTTCTCAT AGACGAGAAA ACGAATCTCC AACAGGAGTT GCATAAGCTG 
CAGCAAGAGT TTGCCCGCCT AGAGCAACAC TCCACGGTGA TCGGAGATGA TGGAGTCTCT 
TTGGGGCCAG TTCAAACAGG ATCTGTGCGC TACAACGAAC TCCGGCGTCA ATTGGACCTA 
CTAAAAGAGG AACTTCTTCA GTCTGAAGGA GCTCGGGAGG ACCTTAAAAT CAAGGCCCAG 
CAACAAGATA CCGATCTGCT GCACATGCAA ATGCGCATAG AGGAATTAAT GAAAAGTTCC 
GCTGAAGTGA CCACACTGAA GGATGAAGTG GATGTTCTGC GGGAGTCCAA CGACAAGCTT 
AAGATATGCG AGGCCCAGCT TGACACGTAT AAAAAGAAGC TAGAAGACTA TAACGACCTG 
AAAAAACAAG TAAAAATTCT GGAGGAGCGT AGCGCCGACT ATGTGCAGCA GAATGCGCAG 
TTTGAGGAGG ATGCCAAACG ATATGCCAAC ACCAAAGGCC AAGTGGAGCT GTTCAAGAAA 
GAGATTCAAG ACCTTCATGC CAAGCTGGAC GCCGAGAGCA GCAAGAATGT AAAGTTGGAG 
TTTGATAACA AGAATTTGGA AAGCAAGAAC CTGGCTCTGC AGCGGGCCAA AGACAGTTTG 
CTCAAGGAGC GCGACAACCT TCGCGAGGCA GTCGACGAGC TGAAATGCGG ACAATTGTCC 
TCGAACACAG CGCTGACAGG CACCACGGTT TCCAGGGAAC TGCAACCGTC GGCCACAGTG 
GAAAAACTAC AGCGACTGGA GGCGGAGAAC AAGGCTCTGC GCGAGGGACA GGGTGGCCAG 
ACAGCTTTGG CGCAACTGCT GGACGATGCC AATAAACGCT GCGAGAATTT GCGTGAGCAG 
CTAAAAACAG CCAATGAACG CATTCTTTCC CTGTCGCATG CTTCTCAAAG CGATGATCCC 
ATTTTAAAAG AGAGCGAGTT TGGTAAACAA ATCAAACAGT TAATGGAGCT AAATGAACAA 
AAGACACTCC AACTGGAGGA AGCAGTTACC CAGAGCACGT CTCTGCAATG CAAGGTGACT 
CAGCTGGAAA CCAATTTATC AGCGCGTGAG CAGGAAATTC TGGTGTATGA CGCCAAATAC 
CGAAAATGCG TGGAGAAAGC TAAGGAGGTG ATCAAAAGCA TAGATCCTCG AATTGCCAGT 
GCTCTGGATG CTAGCGTACT GGAAAAGAGT GCTGATCTCG TCGAGGAGGA GCCCAAGCCG 
AAGATGAGTG TGATGGAGGA GCAACTCATG ACCTCCGCTT TCTACAGACT GGGTGTCAAT 
GCCCAGCGTG ATGCAATCGA CTCTAAGCTG GCAATATTGA TGGGCTCTGG CCAGACATTC 
CTTGCCCGCC AGCGGCAGTC CGCGCCCCGC AAATCCCTAA GTGCAATGAA ATCAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hook cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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