tos cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tos
Entrez Gene ID	35119
Full Name	tosca
Synonyms	CG10387,DmTosca,Dmel\CG10387,Tosca
General protein information	Preferred Names tosca Names CG10387 gene product from transcript CG10387-RA CG10387-PA tos-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 36F11-36F11

mRNA and Protein(s)

mRNA	Protein	Name
NM_057797.4	NP_477145.1	tosca

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tos gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tos cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14910	NM_057797.4 Latest version!	Drosophila melanogaster tosca (tos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tos ( NM_057797.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477145.1 Drosophila melanogaster tosca (tos), mRNA.

Price & Availability↑

CloneID	OFa14910
Clone ID Related Accession (Same CDS sequence)	NM_057797.4
Accession Version	NM_057797.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tosca
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057797.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCATTA CCGGCCTAAT TCCCTTCGTG GGCAAGGCCT CCTCGCAGCT GCATCTAAAA 
GACATTCGCG GCAGCACAGT GGCCGTGGAC ACATATTGCT GGCTACACAA GGGAGTCTTC 
GGCTGTGCGG AGAAGTTGGC CCGCGGCGAG GATACGGATG TTTATATACA ATACTGCTTG 
AAGTATGTGA ACATGCTGCT GTCCTACGAC ATCAAGCCCA TTCTGGTCTT CGATGGGCAG 
CACTTGCCGG CCAAGGCTTT GACCGAAAAG CGGAGAAGGG ACTCCAGGAA GCAGAGCAAA 
GAGCGGGCGG CGGAACTCCT TCGATTGGGT CGCATCGAGG AGGCCCGATC CCATATGCGA 
CGCTGCGTGG ATGTCACCCA CGACATGGCG TTGCGGTTGA TCCGGGAATG CCGGAGCCGG 
AATGTTGACT GCATTGTGGC GCCCTACGAG GCCGATGCCC AAATGGCCTG GCTGAATAGA 
GCAGATGTGG CCCAGTACAT CATCACCGAG GACTCGGACT TAACGCTTTT TGGAGCCAAG 
AACATCATAT TCAAGCTGGA CCTCAACGGC AGCGGCCTGC TGGTGGAGGC GGAAAAACTT 
CACCTGGCCA TGGGCTGCAC GGAGGAGAAG TACCACTTTG ACAAGTTCCG GCGCATGTGC 
ATCCTATCCG GCTGTGATTA CCTGGACTCA CTGCCTGGCA TCGGACTGGC CAAGGCGTGC 
AAATTTATAC TAAAAACGGA ACAGGAAGAC ATGCGAATAG CATTAAAAAA GATTCCAAGT 
TACCTCAATA TGCGCAATCT TGAGGTAGAT GACGACTATA TTGAAAACTT CATGAAAGCG 
GAGGCCACCT TCAGGCACAT GTTTATCTAC AACCCGCTAG AGCGTCGCAT GCAGCGGTTG 
TGTGCCCTCG AAGATTATGA AACTGATGAG CGCTACTGCA GCAATGCTGG CACCTTGCTG 
GAGGATAGCG AGCAGGCTTT GCACTTGGCC CTAGGCAACT TGAACCCCTT CTCTATGAAG 
CGACTGGACT CTTGGACACC GGAAAAGGCA TGGCCAACGC CGAAGAACGT GAAACGATCC 
AAGCACAAAA GTATTTGGCA AACGAATTTT CAAAGCGAAA ACACTCACAC GCCGAAGAAA 
GAAAATCCGT GTGCCTTGTT CTTCAAAAAA GTGGATTTTG TGGGCAAAAC TCTAAACGAG 
GAAATCGAAG CTAATCAGCG ACTGGAACAG GCTAAACAAA CGGAGGCCGA GTTGTTTAAC 
ATGTACAGTT TCAAAGCCAA AAGGAGGAGA AGTCCGAGCA GGGAAGATTC TGTAGATCAA 
GAACGTACAC CTCCTCCGTC GCCGGTGCAC AAAAGTCGGC ACAATCCATT TGCCAAGGAA 
AGGACTGGAG AAGAAGCTAA TCAGCGATCC CCAGTAGTTT GTGAGAATGC CTCTTTGCTG 
CGTTTGCTTA GTCCGAAAAA GGCAAGTCCG TTGGATGGAG AAGCTGGTGT AAAAAAGGTT 
GATTCGCTTA AAAGAAGCAT ATTCGCCAAG GAACAAGTTC AGATCCGAAG TCGCTTCTTC 
GCCACGCAGG ATGAACAGAC AAGGCTTCAA AGGGAGCACT TAAGAGATAC GGAAAATGAC 
GATATGGATG AGCAGAAGCT GAGCTCTCAC TCAGGCCATA AGAAACTAAG ACTAGTATGT 
AAAGACATTC CAGGGAAGAA CCCAATAAGA CAACGTTGTA GTTCACAAAT CAGTGACGGT 
GAAACAGATA CAGATACCAC AGCCTCTTCT CTTTTGGAAT CACAAGATAA AGGTGTTCCT 
TCGCCTTTAG AATCCCAAGA AGATCTCAAC AACTCTCAAC CCCAAATACC AACTGAAGGC 
AATACCAATT CAACAACTAT TCGCATTAAG TCACTGGATT TACTTCTGGA AAACTCTCCG 
GAACCCACTC AGGAATCCGA CAGGAACAAT AATGATGCCA TCATTCTGCT ATCGGACGAC 
AGCTGTAGCT CGGATCAGAG AGCATCATCT ACCTCATCCT CCAGCCAGCA ACGCCAGAAC 
TTTTTGCCAA CCAGCAAACG AAGAGTGGGG CTGAGTAAAC CCTCCACTGC CAAGAAGGGC 
ACTCCTAAAT CTAGGACGAA TGGAAAACTG GGTGCCGTGA GCCAGAATCA AACCAAGCTC 
AGCATGTTTG GCTTCCAGAC GAAACCTGTC CTCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477145.1
CDS	118..2316
Translation	MGITGLIPFVGKASSQLHLKDIRGSTVAVDTYCWLHKGVFGCAEKLARGEDTDVYIQYCLKYVNMLLSYDIKPILVFDGQHLPAKALTEKRRRDSRKQSKERAAELLRLGRIEEARSHMRRCVDVTHDMALRLIRECRSRNVDCIVAPYEADAQMAWLNRADVAQYIITEDSDLTLFGAKNIIFKLDLNGSGLLVEAEKLHLAMGCTEEKYHFDKFRRMCILSGCDYLDSLPGIGLAKACKFILKTEQEDMRIALKKIPSYLNMRNLEVDDDYIENFMKAEATFRHMFIYNPLERRMQRLCALEDYETDERYCSNAGTLLEDSEQALHLALGNLNPFSMKRLDSWTPEKAWPTPKNVKRSKHKSIWQTNFQSENTHTPKKENPCALFFKKVDFVGKTLNEEIEANQRLEQAKQTEAELFNMYSFKAKRRRSPSREDSVDQERTPPPSPVHKSRHNPFAKERTGEEANQRSPVVCENASLLRLLSPKKASPLDGEAGVKKVDSLKRSIFAKEQVQIRSRFFATQDEQTRLQREHLRDTENDDMDEQKLSSHSGHKKLRLVCKDIPGKNPIRQRCSSQISDGETDTDTTASSLLESQDKGVPSPLESQEDLNNSQPQIPTEGNTNSTTIRIKSLDLLLENSPEPTQESDRNNNDAIILLSDDSCSSDQRASSTSSSSQQRQNFLPTSKRRVGLSKPSTAKKGTPKSRTNGKLGAVSQNQTKLSMFGFQTKPVLK

Target ORF information:

RefSeq Version	NM_057797.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tosca (tos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057797.4

ORF Insert Sequence:

ATGGGCATTA CCGGCCTAAT TCCCTTCGTG GGCAAGGCCT CCTCGCAGCT GCATCTAAAA 
GACATTCGCG GCAGCACAGT GGCCGTGGAC ACATATTGCT GGCTACACAA GGGAGTCTTC 
GGCTGTGCGG AGAAGTTGGC CCGCGGCGAG GATACGGATG TTTATATACA ATACTGCTTG 
AAGTATGTGA ACATGCTGCT GTCCTACGAC ATCAAGCCCA TTCTGGTCTT CGATGGGCAG 
CACTTGCCGG CCAAGGCTTT GACCGAAAAG CGGAGAAGGG ACTCCAGGAA GCAGAGCAAA 
GAGCGGGCGG CGGAACTCCT TCGATTGGGT CGCATCGAGG AGGCCCGATC CCATATGCGA 
CGCTGCGTGG ATGTCACCCA CGACATGGCG TTGCGGTTGA TCCGGGAATG CCGGAGCCGG 
AATGTTGACT GCATTGTGGC GCCCTACGAG GCCGATGCCC AAATGGCCTG GCTGAATAGA 
GCAGATGTGG CCCAGTACAT CATCACCGAG GACTCGGACT TAACGCTTTT TGGAGCCAAG 
AACATCATAT TCAAGCTGGA CCTCAACGGC AGCGGCCTGC TGGTGGAGGC GGAAAAACTT 
CACCTGGCCA TGGGCTGCAC GGAGGAGAAG TACCACTTTG ACAAGTTCCG GCGCATGTGC 
ATCCTATCCG GCTGTGATTA CCTGGACTCA CTGCCTGGCA TCGGACTGGC CAAGGCGTGC 
AAATTTATAC TAAAAACGGA ACAGGAAGAC ATGCGAATAG CATTAAAAAA GATTCCAAGT 
TACCTCAATA TGCGCAATCT TGAGGTAGAT GACGACTATA TTGAAAACTT CATGAAAGCG 
GAGGCCACCT TCAGGCACAT GTTTATCTAC AACCCGCTAG AGCGTCGCAT GCAGCGGTTG 
TGTGCCCTCG AAGATTATGA AACTGATGAG CGCTACTGCA GCAATGCTGG CACCTTGCTG 
GAGGATAGCG AGCAGGCTTT GCACTTGGCC CTAGGCAACT TGAACCCCTT CTCTATGAAG 
CGACTGGACT CTTGGACACC GGAAAAGGCA TGGCCAACGC CGAAGAACGT GAAACGATCC 
AAGCACAAAA GTATTTGGCA AACGAATTTT CAAAGCGAAA ACACTCACAC GCCGAAGAAA 
GAAAATCCGT GTGCCTTGTT CTTCAAAAAA GTGGATTTTG TGGGCAAAAC TCTAAACGAG 
GAAATCGAAG CTAATCAGCG ACTGGAACAG GCTAAACAAA CGGAGGCCGA GTTGTTTAAC 
ATGTACAGTT TCAAAGCCAA AAGGAGGAGA AGTCCGAGCA GGGAAGATTC TGTAGATCAA 
GAACGTACAC CTCCTCCGTC GCCGGTGCAC AAAAGTCGGC ACAATCCATT TGCCAAGGAA 
AGGACTGGAG AAGAAGCTAA TCAGCGATCC CCAGTAGTTT GTGAGAATGC CTCTTTGCTG 
CGTTTGCTTA GTCCGAAAAA GGCAAGTCCG TTGGATGGAG AAGCTGGTGT AAAAAAGGTT 
GATTCGCTTA AAAGAAGCAT ATTCGCCAAG GAACAAGTTC AGATCCGAAG TCGCTTCTTC 
GCCACGCAGG ATGAACAGAC AAGGCTTCAA AGGGAGCACT TAAGAGATAC GGAAAATGAC 
GATATGGATG AGCAGAAGCT GAGCTCTCAC TCAGGCCATA AGAAACTAAG ACTAGTATGT 
AAAGACATTC CAGGGAAGAA CCCAATAAGA CAACGTTGTA GTTCACAAAT CAGTGACGGT 
GAAACAGATA CAGATACCAC AGCCTCTTCT CTTTTGGAAT CACAAGATAA AGGTGTTCCT 
TCGCCTTTAG AATCCCAAGA AGATCTCAAC AACTCTCAAC CCCAAATACC AACTGAAGGC 
AATACCAATT CAACAACTAT TCGCATTAAG TCACTGGATT TACTTCTGGA AAACTCTCCG 
GAACCCACTC AGGAATCCGA CAGGAACAAT AATGATGCCA TCATTCTGCT ATCGGACGAC 
AGCTGTAGCT CGGATCAGAG AGCATCATCT ACCTCATCCT CCAGCCAGCA ACGCCAGAAC 
TTTTTGCCAA CCAGCAAACG AAGAGTGGGG CTGAGTAAAC CCTCCACTGC CAAGAAGGGC 
ACTCCTAAAT CTAGGACGAA TGGAAAACTG GGTGCCGTGA GCCAGAATCA AACCAAGCTC 
AGCATGTTTG GCTTCCAGAC GAAACCTGTC CTCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tos cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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