bsf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bsf
Entrez Gene ID	35100
Full Name	bicoid stability factor
Synonyms	BSF,CG10302,DmLrpprc1,Dmel\CG10302,l(2)SH1181,l(2)SH2 1181
General protein information	Preferred Names bicoid stability factor Names CG10302 gene product from transcript CG10302-RA CG10302-PA bicoid stabilization factor bsf-PA lethal (2) SH1181
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 36F5-36F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_078872.5	NP_523596.2	bicoid stability factor

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRPPRC	NP_573566.2
Mus musculus (house mouse)	Lrpprc	NP_082509.2
Rattus norvegicus (Norway rat)	Lrpprc	NP_001008519.1
Danio rerio (zebrafish)	lrpprc	NP_001136064.1
Xenopus tropicalis (tropical clawed frog)	lrpprc	NP_001039203.1
Pan troglodytes (chimpanzee)	LRPPRC	XP_001143797.1
	LRPPRC	XP_001107298.2
Canis lupus familiaris (dog)	LRPPRC	XP_531800.2
Gallus gallus (chicken)	LRPPRC	XP_001234903.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008770	XP_557938.3
Bos taurus (cattle)	LRPPRC	XP_002691297.2
Drosophila melanogaster (fruit fly)	bsf	NP_523596.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Although the C region of the osk 3' UTR is required for the noncoding function, BSF binding does not appear to be essential for that function.
Title: RNA sequences required for the noncoding function of oskar RNA also mediate regulation of Oskar protein expression by Bicoid Stability Factor.

Epistasis studies showed that Mbl silencing was sufficient to induce a subcellular redistribution of TBPH and BSF proteins in the muscle, which mimicked the effect of i(CTG)480 expression.
Title: Muscleblind, BSF and TBPH are mislocalized in the muscle sarcomere of a Drosophila myotonic dystrophy model.

it reports of a novel function of BSF in flies and demonstrates that it has an important intra-mitochondrial role, which is essential for maintaining mtDNA gene expression and oxidative phosphorylation.
Title: The bicoid stability factor controls polyadenylation and expression of specific mitochondrial mRNAs in Drosophila melanogaster.

activation of the zygotic genome starts from intronless genes sharing a common cis-element. This cis-element interacts with a single protein, Bicoid stability factor, & acts as potent enhancer capable of timing activity of an exogenous transactivator.
Title: Unmasking activation of the zygotic genome using chromosomal deletions in the Drosophila embryo.

The following bsf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bsf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14891	NM_078872.5 Latest version!	Drosophila melanogaster bicoid stability factor (bsf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bsf ( NM_078872.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523596.2 Drosophila melanogaster bicoid stability factor (bsf), mRNA.

Price & Availability↑

CloneID	OFa14891
Clone ID Related Accession (Same CDS sequence)	NM_078872.5
Accession Version	NM_078872.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bicoid stability factor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078872.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCCA TCCTGAGGAC GGGCAAACTG CTGCGCTACT TCGCCGGCTT CACACGCAAT 
GTGCTGGTGA ACTCGGTTCG CGAGAACGAG ACGAGCAATC TGCTGAACAA TGCGCCCTGC 
ATGTGCGGCC AGTTCCAAAA TGGTTTTGCC ACGAATGCGG CGGCCAAAGC TGAGTTGAGT 
TTGGATAAAC AGATTCGCCG GCTGGACCAG GATGTTCGTC GCATTGGGCG CATCTCCAGA 
CGCGATCTGG AGGAGGTGCT CGATGAGATC CGCTCACACA GGACGGCCAC CAGCTCGCAA 
TCGCTGCTGG TGATTCGCTG CTGCGGCAAC CTGGTACCGG AGGAGCTTCC CGAGGTCAGA 
ACTGCCTTGG TGCAGGAGAT CTGGAAGACG CTGAATGCGC TGAATGTACC CATGGACATC 
TCGCACTACA ATGCCCTGCT TCGCGTTTAC CTGGAGAACG AGCATGGGTT TGCTCCCACC 
GATTTTCTGG CAGAGATCGA GGCGAAGGGC ATCGAGCCCA ATAGGGTGAC CTATCAGCGA 
TTGATTGCTC GCTATTGTCA GCAGGGAGAC ATTGAAGGAG CCACTAGGAT TCTGGAATTC 
ATGCGCGCCA AAAGTCTACC CGTCAACGAA AATGTCTTTA ATTCACTTAT CCTGGGTCAC 
TCGCAGGCCA ATGATTTGGA GTCCGCCAAG GGTATCTTGG GAGTGATGAT GCAGGCCGGC 
TTGGAGCCCA GTGCTGACAC CTACACCACT CTCCTGTGCG CCTTTGCGCG CCACGGAGAC 
CTGGCTGCCG TAAAGGAAAC CCTAGCTGAG TGTGAGCAGA AGGAGATCAT TTTGCTGGAC 
AAGGATCTGC TGGACATTGT GTACACTCTT ACCGTCAACG GCAATGGTGA ACACGTGGAT 
GACGTTCTGA CCAAGCTTCG TCTCTCGCCT GGTTTTAATC AGGATGCAGT GAACGTGATC 
CTTCGGCTGG TCAACAAGGG ACACGAGGAT GTGGGGCTGA AGCTCCTTCG CGTGATGCCA 
AGGAGCAGTC GTGTGAACGG TGAGCCCGTT GATGTGGGAG CTTTCTTCAT CAGGCAGATG 
GTTAAGGCCA ATCGGCCAGT GGAGAAGATC TTGTCCATCT GCAAGAGCCT GCAATCAGAG 
GGGCTTAATC CCAAGGCTCT GACTATTGCC ACCGAAGCTG GCTTGACCAA TGGCGTAATC 
AATAATGCGC TTCCCTTGCT GCAGGAAATG AAAAATGCTG GCTTGCCCAT CAGGCAGCAC 
TACTTCTGGC CTTTGATCTG TTCCGCGGAA TCTAATCAAG TCTTGGAAAT CGTACGTCGC 
ATGCAGCAGG AGTTCTCAGT TTTCCCCAAC TCCGAAACGG TCAGAGATTA TGTCATTCCC 
AACTTGAAGG AAAAGAACTG GGAAAGAATC GTCACCTCTC TGAGGGATGC TGGGGTTCCC 
AACTCCACTG CTGTCACCTC GGCCGTCTAC TCCGCATTAG TGACCCACCA GATTGCCGAA 
GCAGCCAAGA TTATGGAGAA GAACAGAGCT TACTACGTGC CCTTCTTGTT CAAACAACCT 
TTGATTCTGG CCCTCAGCCA TACGAACGAT TATGCCTCCT TCATCCGATG TGTCCGCCAA 
ATTCACGAAG GATTGCAATT CAGGCAAGGC AAGGATGAGG AAGTTGAGCA GGTAGAAGGC 
GTGGCTGCCG TTCCGACGGA GCGCATCACT CCCGATGTTG TAGGTGCAAT AGTCCAGGAG 
GCAACCACCT ATTTCCGCCG CGATCGCGGG GCCACGTTGG AGAAAATCCT CAAAGGGTTG 
GTCAAGCAGG GCTTGTCCAT TAGCAGTGAC AAAGCCACCC AACTATCCGA GCAGTTGGGT 
TCCGAACTGA CTCCAAAGAT TTCCGAACTT CTGGGTAAAC TTAGCTCGGG AGAACTGGAG 
CCAGTTCCTC TTCCAAACAG CGGCAAGAGA AGCCTGGATT CGCTGTCCAT CAATGAACTC 
GAGCGATTCA TTGTGAATGT GGAGGGCAAG GGAGAAAATG CCAACAACAT CAAGAGGCAA 
CTCCTCAACG CCTGTTTCCG TTCGCAAAAC TTGGAGAAGA CCCTCCAGGT CATCGAGAAA 
CTCGAGGCCG AAAAATTCCA AATCCCCGTT GGCATTTATG CCCAGCTCAT CGATCTGAAC 
ACGCATCACA AGAAAAGCTC TGAGGCCTTA GAAATCTATG GCAAACTTAA AGCAAAGGAC 
GCCACTTTCC AGTTGGATAA CTTGAAGGCG GTTCGACTGG CTGATCTTCT CTTGCAAGAG 
GAACGCGTGG ATGCTGCTTT TAAGATACTG GAGGAGAACA AGAAGGAAGT TCCCGTAGCC 
GAGGCCGAAG GAAGCTATAA TTACATGAGC ACCGTGTGGA GAATTTTGAA CTCACTAGCC 
GAGGCCGGTC AGCCGGAGAG GCTGCGCAAA TTGTTCGACG TACTGGTGGG GGCCAATTAT 
ATTGTGCCCA CCAATGTGCT GCTGGGTCCA CTGATCAAGG TTCACCTGGT CAAGGATGAT 
ATTCCCAAGG CAATCGAAGC CTTCGAGGAG ATTTGCCAGA AGTATAAGTC CACGCCTTGG 
AAGAACGAGC TGGCTTGCCG TCTAATACAG AAAGAAGATG CTGCCAATCT GCAAAAGTTA 
ACAGACTTGA GCACTGGAAT CCATGGTGAA GTAAACAGCC TTTACGATCT GGTCTTCTCC 
TTTGTGGAGT GCGGCCGTGT GCGGCAGGCC AGGAAGATCC TGGAAACCCC CGGACTGCGT 
ACCCGTCCGC AAAGAATAAA CAGTGCCTGT GATCGTTACA AGAATGAGGG ACTACTGCAA 
CCGCTGGAAG GTCTTATTGA AGCCACGAAG GACCTGGGAC ATATCGACAG GAATAAGATT 
TACTACACGC TCCTCTTGAG CTACGACAAG GCAGATGAGG CGGAGAAGGC TTTGGGACTG 
TGGACTAAAA TGCAGGAGGA GAATGTGACA CCAAACGATG CTTTCCTTTT GAAATTGGCA 
GAAATCCTGA GTAAGAAAAA TATTGACGTG CCTTTTGTGG TGCCAGAGAC CCAAAACGAA 
CAAGCCAAGG CCAAAAAAAG CAAAGCGAAA GATGCAACGA CAACAGAAGC AGCCACAGAA 
ACAGCTAAAG TAGCAGAACA GCCCAAGGAG AAGGCTGCGA AAAAGGAGCC AGCTAAAACG 
GAAACAGCAA AGCCACCCAA AACCGCCTCC CATTTGTCCG GCTTCCGCAA GGCAATCCAG 
GCCAATGATC CAGATGCAGC CATCTCGCAT AAACAAAGTG TTCTGAGTGG CGAGAAAATT 
GGAGCTTTGG ACACCTCTCG ACTGATCGAG CTCCTGGTTC GAGCTGATCG CTTAACGGAG 
GCCACCAAGT ATGTCGAGGA GCTCCTGGGC GATAAACTGC ACCCGCAGCC CAAGATCTTC 
AAGTTTTACC TGAACAAAAT TGCCGCTGCA GGCGATCTGG AAACAATGCA GAGAATTGGC 
CAGCAGCTGA ATGACGAGCA GAAGCGTCTG ATTTCCTTTG ACAATCGGTT TTGTCATGCC 
AATATAGTGG CCGGAAAGGC GGAGGAGTTC CTGAAGCACT TGACCACAGA AATCGATGCG 
GCCAAAACTC CCGAGGAGTC AACCAAGCTG GCTGAGAAGT TCCCACGCGG AGGAGCAGTT 
GGCATTTTGG AAAAACACCC GGAATTGGTG CCGCAATATC AATCCCTCGC TGAGAAGTTT 
GCTGCTCACA ATCAACTAGG ACCCATGAAT GTCCTGTGGA TGCATTTGAT TTCCACTGGC 
CAGGAGGCGG CTTCTAAGGA GATTTGGGAT AAGCACCTGT CCAATGCCCC AAGGCTGATG 
TTCCAGCGCG TTTTGCAGAC GGCTCGCGAG CAGCAGGACG AGAAGCTCGC CTCCACAGTC 
ATTTCCCAGC TGAAGAACAG CAAAATCTCC GAGGGCGCGA TCGGAAACGC GTACTCCTGC 
CTCATTGACA TCCAGACTAC CAAGGGCAAC TCCGACAAGG CCATGGTTGT CTTGGCCAAT 
GCCATTAAGG ACGTATCCTT GGAAAACATC AACAGGACAG CTCTGCTGCG TCTGAAACAG 
GCGGTGGAGG AGAAATCCCA GAAGTTCCCC TACACGATCC CCGAGAAGCG GACGAAGGCC 
GACGACTCCA GCTCGTCCTC CTCTTCATCC TCCAGCGACG ACGACGTGTC GCCATCGGTA 
CCGGAAACTG TTCCACCCAA ACCGGACAGC AAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523596.2
CDS	102..4340
Translation	MASILRTGKLLRYFAGFTRNVLVNSVRENETSNLLNNAPCMCGQFQNGFATNAAAKAELSLDKQIRRLDQDVRRIGRISRRDLEEVLDEIRSHRTATSSQSLLVIRCCGNLVPEELPEVRTALVQEIWKTLNALNVPMDISHYNALLRVYLENEHGFAPTDFLAEIEAKGIEPNRVTYQRLIARYCQQGDIEGATRILEFMRAKSLPVNENVFNSLILGHSQANDLESAKGILGVMMQAGLEPSADTYTTLLCAFARHGDLAAVKETLAECEQKEIILLDKDLLDIVYTLTVNGNGEHVDDVLTKLRLSPGFNQDAVNVILRLVNKGHEDVGLKLLRVMPRSSRVNGEPVDVGAFFIRQMVKANRPVEKILSICKSLQSEGLNPKALTIATEAGLTNGVINNALPLLQEMKNAGLPIRQHYFWPLICSAESNQVLEIVRRMQQEFSVFPNSETVRDYVIPNLKEKNWERIVTSLRDAGVPNSTAVTSAVYSALVTHQIAEAAKIMEKNRAYYVPFLFKQPLILALSHTNDYASFIRCVRQIHEGLQFRQGKDEEVEQVEGVAAVPTERITPDVVGAIVQEATTYFRRDRGATLEKILKGLVKQGLSISSDKATQLSEQLGSELTPKISELLGKLSSGELEPVPLPNSGKRSLDSLSINELERFIVNVEGKGENANNIKRQLLNACFRSQNLEKTLQVIEKLEAEKFQIPVGIYAQLIDLNTHHKKSSEALEIYGKLKAKDATFQLDNLKAVRLADLLLQEERVDAAFKILEENKKEVPVAEAEGSYNYMSTVWRILNSLAEAGQPERLRKLFDVLVGANYIVPTNVLLGPLIKVHLVKDDIPKAIEAFEEICQKYKSTPWKNELACRLIQKEDAANLQKLTDLSTGIHGEVNSLYDLVFSFVECGRVRQARKILETPGLRTRPQRINSACDRYKNEGLLQPLEGLIEATKDLGHIDRNKIYYTLLLSYDKADEAEKALGLWTKMQEENVTPNDAFLLKLAEILSKKNIDVPFVVPETQNEQAKAKKSKAKDATTTEAATETAKVAEQPKEKAAKKEPAKTETAKPPKTASHLSGFRKAIQANDPDAAISHKQSVLSGEKIGALDTSRLIELLVRADRLTEATKYVEELLGDKLHPQPKIFKFYLNKIAAAGDLETMQRIGQQLNDEQKRLISFDNRFCHANIVAGKAEEFLKHLTTEIDAAKTPEESTKLAEKFPRGGAVGILEKHPELVPQYQSLAEKFAAHNQLGPMNVLWMHLISTGQEAASKEIWDKHLSNAPRLMFQRVLQTAREQQDEKLASTVISQLKNSKISEGAIGNAYSCLIDIQTTKGNSDKAMVVLANAIKDVSLENINRTALLRLKQAVEEKSQKFPYTIPEKRTKADDSSSSSSSSSSDDDVSPSVPETVPPKPDSKA

Target ORF information:

RefSeq Version	NM_078872.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bicoid stability factor (bsf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078872.5

ORF Insert Sequence:

ATGGCATCCA TCCTGAGGAC GGGCAAACTG CTGCGCTACT TCGCCGGCTT CACACGCAAT 
GTGCTGGTGA ACTCGGTTCG CGAGAACGAG ACGAGCAATC TGCTGAACAA TGCGCCCTGC 
ATGTGCGGCC AGTTCCAAAA TGGTTTTGCC ACGAATGCGG CGGCCAAAGC TGAGTTGAGT 
TTGGATAAAC AGATTCGCCG GCTGGACCAG GATGTTCGTC GCATTGGGCG CATCTCCAGA 
CGCGATCTGG AGGAGGTGCT CGATGAGATC CGCTCACACA GGACGGCCAC CAGCTCGCAA 
TCGCTGCTGG TGATTCGCTG CTGCGGCAAC CTGGTACCGG AGGAGCTTCC CGAGGTCAGA 
ACTGCCTTGG TGCAGGAGAT CTGGAAGACG CTGAATGCGC TGAATGTACC CATGGACATC 
TCGCACTACA ATGCCCTGCT TCGCGTTTAC CTGGAGAACG AGCATGGGTT TGCTCCCACC 
GATTTTCTGG CAGAGATCGA GGCGAAGGGC ATCGAGCCCA ATAGGGTGAC CTATCAGCGA 
TTGATTGCTC GCTATTGTCA GCAGGGAGAC ATTGAAGGAG CCACTAGGAT TCTGGAATTC 
ATGCGCGCCA AAAGTCTACC CGTCAACGAA AATGTCTTTA ATTCACTTAT CCTGGGTCAC 
TCGCAGGCCA ATGATTTGGA GTCCGCCAAG GGTATCTTGG GAGTGATGAT GCAGGCCGGC 
TTGGAGCCCA GTGCTGACAC CTACACCACT CTCCTGTGCG CCTTTGCGCG CCACGGAGAC 
CTGGCTGCCG TAAAGGAAAC CCTAGCTGAG TGTGAGCAGA AGGAGATCAT TTTGCTGGAC 
AAGGATCTGC TGGACATTGT GTACACTCTT ACCGTCAACG GCAATGGTGA ACACGTGGAT 
GACGTTCTGA CCAAGCTTCG TCTCTCGCCT GGTTTTAATC AGGATGCAGT GAACGTGATC 
CTTCGGCTGG TCAACAAGGG ACACGAGGAT GTGGGGCTGA AGCTCCTTCG CGTGATGCCA 
AGGAGCAGTC GTGTGAACGG TGAGCCCGTT GATGTGGGAG CTTTCTTCAT CAGGCAGATG 
GTTAAGGCCA ATCGGCCAGT GGAGAAGATC TTGTCCATCT GCAAGAGCCT GCAATCAGAG 
GGGCTTAATC CCAAGGCTCT GACTATTGCC ACCGAAGCTG GCTTGACCAA TGGCGTAATC 
AATAATGCGC TTCCCTTGCT GCAGGAAATG AAAAATGCTG GCTTGCCCAT CAGGCAGCAC 
TACTTCTGGC CTTTGATCTG TTCCGCGGAA TCTAATCAAG TCTTGGAAAT CGTACGTCGC 
ATGCAGCAGG AGTTCTCAGT TTTCCCCAAC TCCGAAACGG TCAGAGATTA TGTCATTCCC 
AACTTGAAGG AAAAGAACTG GGAAAGAATC GTCACCTCTC TGAGGGATGC TGGGGTTCCC 
AACTCCACTG CTGTCACCTC GGCCGTCTAC TCCGCATTAG TGACCCACCA GATTGCCGAA 
GCAGCCAAGA TTATGGAGAA GAACAGAGCT TACTACGTGC CCTTCTTGTT CAAACAACCT 
TTGATTCTGG CCCTCAGCCA TACGAACGAT TATGCCTCCT TCATCCGATG TGTCCGCCAA 
ATTCACGAAG GATTGCAATT CAGGCAAGGC AAGGATGAGG AAGTTGAGCA GGTAGAAGGC 
GTGGCTGCCG TTCCGACGGA GCGCATCACT CCCGATGTTG TAGGTGCAAT AGTCCAGGAG 
GCAACCACCT ATTTCCGCCG CGATCGCGGG GCCACGTTGG AGAAAATCCT CAAAGGGTTG 
GTCAAGCAGG GCTTGTCCAT TAGCAGTGAC AAAGCCACCC AACTATCCGA GCAGTTGGGT 
TCCGAACTGA CTCCAAAGAT TTCCGAACTT CTGGGTAAAC TTAGCTCGGG AGAACTGGAG 
CCAGTTCCTC TTCCAAACAG CGGCAAGAGA AGCCTGGATT CGCTGTCCAT CAATGAACTC 
GAGCGATTCA TTGTGAATGT GGAGGGCAAG GGAGAAAATG CCAACAACAT CAAGAGGCAA 
CTCCTCAACG CCTGTTTCCG TTCGCAAAAC TTGGAGAAGA CCCTCCAGGT CATCGAGAAA 
CTCGAGGCCG AAAAATTCCA AATCCCCGTT GGCATTTATG CCCAGCTCAT CGATCTGAAC 
ACGCATCACA AGAAAAGCTC TGAGGCCTTA GAAATCTATG GCAAACTTAA AGCAAAGGAC 
GCCACTTTCC AGTTGGATAA CTTGAAGGCG GTTCGACTGG CTGATCTTCT CTTGCAAGAG 
GAACGCGTGG ATGCTGCTTT TAAGATACTG GAGGAGAACA AGAAGGAAGT TCCCGTAGCC 
GAGGCCGAAG GAAGCTATAA TTACATGAGC ACCGTGTGGA GAATTTTGAA CTCACTAGCC 
GAGGCCGGTC AGCCGGAGAG GCTGCGCAAA TTGTTCGACG TACTGGTGGG GGCCAATTAT 
ATTGTGCCCA CCAATGTGCT GCTGGGTCCA CTGATCAAGG TTCACCTGGT CAAGGATGAT 
ATTCCCAAGG CAATCGAAGC CTTCGAGGAG ATTTGCCAGA AGTATAAGTC CACGCCTTGG 
AAGAACGAGC TGGCTTGCCG TCTAATACAG AAAGAAGATG CTGCCAATCT GCAAAAGTTA 
ACAGACTTGA GCACTGGAAT CCATGGTGAA GTAAACAGCC TTTACGATCT GGTCTTCTCC 
TTTGTGGAGT GCGGCCGTGT GCGGCAGGCC AGGAAGATCC TGGAAACCCC CGGACTGCGT 
ACCCGTCCGC AAAGAATAAA CAGTGCCTGT GATCGTTACA AGAATGAGGG ACTACTGCAA 
CCGCTGGAAG GTCTTATTGA AGCCACGAAG GACCTGGGAC ATATCGACAG GAATAAGATT 
TACTACACGC TCCTCTTGAG CTACGACAAG GCAGATGAGG CGGAGAAGGC TTTGGGACTG 
TGGACTAAAA TGCAGGAGGA GAATGTGACA CCAAACGATG CTTTCCTTTT GAAATTGGCA 
GAAATCCTGA GTAAGAAAAA TATTGACGTG CCTTTTGTGG TGCCAGAGAC CCAAAACGAA 
CAAGCCAAGG CCAAAAAAAG CAAAGCGAAA GATGCAACGA CAACAGAAGC AGCCACAGAA 
ACAGCTAAAG TAGCAGAACA GCCCAAGGAG AAGGCTGCGA AAAAGGAGCC AGCTAAAACG 
GAAACAGCAA AGCCACCCAA AACCGCCTCC CATTTGTCCG GCTTCCGCAA GGCAATCCAG 
GCCAATGATC CAGATGCAGC CATCTCGCAT AAACAAAGTG TTCTGAGTGG CGAGAAAATT 
GGAGCTTTGG ACACCTCTCG ACTGATCGAG CTCCTGGTTC GAGCTGATCG CTTAACGGAG 
GCCACCAAGT ATGTCGAGGA GCTCCTGGGC GATAAACTGC ACCCGCAGCC CAAGATCTTC 
AAGTTTTACC TGAACAAAAT TGCCGCTGCA GGCGATCTGG AAACAATGCA GAGAATTGGC 
CAGCAGCTGA ATGACGAGCA GAAGCGTCTG ATTTCCTTTG ACAATCGGTT TTGTCATGCC 
AATATAGTGG CCGGAAAGGC GGAGGAGTTC CTGAAGCACT TGACCACAGA AATCGATGCG 
GCCAAAACTC CCGAGGAGTC AACCAAGCTG GCTGAGAAGT TCCCACGCGG AGGAGCAGTT 
GGCATTTTGG AAAAACACCC GGAATTGGTG CCGCAATATC AATCCCTCGC TGAGAAGTTT 
GCTGCTCACA ATCAACTAGG ACCCATGAAT GTCCTGTGGA TGCATTTGAT TTCCACTGGC 
CAGGAGGCGG CTTCTAAGGA GATTTGGGAT AAGCACCTGT CCAATGCCCC AAGGCTGATG 
TTCCAGCGCG TTTTGCAGAC GGCTCGCGAG CAGCAGGACG AGAAGCTCGC CTCCACAGTC 
ATTTCCCAGC TGAAGAACAG CAAAATCTCC GAGGGCGCGA TCGGAAACGC GTACTCCTGC 
CTCATTGACA TCCAGACTAC CAAGGGCAAC TCCGACAAGG CCATGGTTGT CTTGGCCAAT 
GCCATTAAGG ACGTATCCTT GGAAAACATC AACAGGACAG CTCTGCTGCG TCTGAAACAG 
GCGGTGGAGG AGAAATCCCA GAAGTTCCCC TACACGATCC CCGAGAAGCG GACGAAGGCC 
GACGACTCCA GCTCGTCCTC CTCTTCATCC TCCAGCGACG ACGACGTGTC GCCATCGGTA 
CCGGAAACTG TTCCACCCAA ACCGGACAGC AAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bsf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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