kel cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	kel
Entrez Gene ID	35084
Full Name	kelch
Synonyms	CG7210,Dm.Ring_canal/KELC_DROME,Dmel\CG7210,Kel,Kelch
General protein information	Preferred Names kelch Names CG7210 gene product from transcript CG7210-RA CG7210-PA CG7210-PB kel-PA kel-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 36E5-36E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_165242.2	NP_724095.1	kelch, isoform A
NM_057241.3	NP_476589.4	kelch, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	KLHL3	NP_059111.2
Gallus gallus (chicken)	KLHL3	XP_414621.4
Pan troglodytes (chimpanzee)	KLHL3	XP_001169820.2
	KLHL3	XP_002804568.1
Danio rerio (zebrafish)	klhl3	XP_696980.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008362	XP_317091.3
Canis lupus familiaris (dog)	KLHL3	XP_005626632.1
Drosophila melanogaster (fruit fly)	kel	NP_476589.4
Bos taurus (cattle)	KLHL3	XP_005209460.1
Mus musculus (house mouse)	Klhl3	NP_001182004.1
Caenorhabditis elegans (roundworm)	kel-1	NP_001254310.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results demonstrate that Kelch promotes ring canal cytoskeleton growth primarily through its activity as cullin-RING E3 ubiquitin ligase component
Title: Actin Cytoskeletal Organization in Drosophila Germline Ring Canals Depends on Kelch Function in a Cullin-RING E3 Ligase.

demonstrate that association of Drosophila KEL with Cullin-3, likely in a cullin-RING ligase, is essential for the growth of Drosophila female germline ring canals.
Title: Drosophila Kelch functions with Cullin-3 to organize the ring canal actin cytoskeleton.

COP9 limits dendritic branching via Cullin3-dependent degradation of the actin-crosslinking BTB-domain protein Kelch
Title: COP9 limits dendritic branching via Cullin3-dependent degradation of the actin-crosslinking BTB-domain protein Kelch.

The following kel gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kel cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14873	NM_165242.2 Latest version!	Drosophila melanogaster kelch (kel), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa14874	NM_057241.3 Latest version!	Drosophila melanogaster kelch (kel), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kel ( NM_165242.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724095.1 Drosophila melanogaster kelch (kel), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14873
Clone ID Related Accession (Same CDS sequence)	NM_165242.2
Accession Version	NM_165242.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kelch, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165242.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATAGCTC TGAGTGCGCT GCTAACCAAA TATACGATCG GTATTATGAG CAACCTAAGC 
AATGGCAACA GCAACAACAA CAACCAGCAG CAGCAGCAAC AACAACAGGG TCAGAACCCA 
CAACAACCAG CACAGAATGA AGGCGGAGCG GGAGCGGAGT TCGTGGCGCC ACCGCCCGGA 
CTAGGTGCCG CAGTGGGCGT GGCAGCCATG CAGCAGCGCA ACCGCCTGCT GCAGCAGCAA 
CAGCAGCAGC ACCACCACCA TCAGAATCCC GCCGCCGAGG GAAGTGGCCT GGAGCGGGGC 
AGCTGCCTTC TGCGCTACGC CAGCCAGAAC TCCCTGGACG AGAGCTCCCA GAAGCACGTC 
CAGCGGCCAA ATGGCAAGGA GCGCGGCACT GTGGGGCAGT ACAGCAATGA ACAGCATACG 
GCGCGCTCCT TCGACGCCAT GAACGAGATG AGAAAACAAA AACAGCTCTG TGATGTGATA 
TTGGTGGCCG ACGATGTGGA GATCCACGCT CACCGCATGG TGCTGGCCTC CTGCAGTCCG 
TACTTCTATG CGATGTTCAC CAGTTTCGAG GAGTCGCGGC AGGCGAGGAT CACCCTCCAG 
AGTGTCGATG CTCGGGCCTT GGAGCTGCTC ATCGACTACG TATACACTGC CACGGTGGAG 
GTAAACGAGG ATAACGTGCA AGTCCTCTTG ACCGCCGCCA ACCTGCTCCA GCTCACGGAT 
GTGCGAGATG CCTGCTGCGA CTTCCTGCAA ACTCAGTTGG ATGCCAGTAA TTGCCTCGGC 
ATCCGGGAGT TTGCCGATAT TCATGCCTGC GTGGAACTTC TCAATTACGC AGAGACCTAC 
ATCGAGCAGC ATTTCAACGA GGTGATCCAG TTCGATGAGT TTCTGAACCT CTCGCACGAG 
CAGGTCATCA GCCTGATTGG CAACGACCGG ATTTCGGTTC CAAATGAGGA GCGCGTATAC 
GAGTGCGTCA TCGCCTGGCT GCGCTACGAT GTTCCTATGC GGGAACAGTT CACCTCGTTG 
CTCATGGAGC ATGTGCGTCT GCCGTTCCTG TCCAAGGAGT ACATCACACA ACGTGTGGAC 
AAGGAGATCC TCCTCGAGGG CAATATCGTG TGCAAGAATC TCATCATTGA GGCATTGACC 
TATCATCTAC TGCCCACTGA GACAAAGTCC GCTAGAACGG TGCCCAGGAA GCCGGTGGGC 
ATGCCCAAGA TACTGCTCGT TATTGGAGGA CAAGCACCCA AGGCTATACG ATCGGTGGAG 
TGGTATGATC TGCGAGAGGA AAAGTGGTAC CAGGCCGCCG AAATGCCCAA CCGCAGATGC 
CGCTCCGGCT TAAGTGTCCT GGGTGACAAA GTCTATGCTG TCGGAGGTTT CAATGGATCA 
CTGCGTGTGC GCACCGTAGA TGTCTACGAT CCTGCCACGG ATCAGTGGGC AAACTGCAGC 
AACATGGAGG CCAGACGATC TACCCTGGGA GTAGCTGTCC TCAATGGTTG CATATACGCC 
GTGGGTGGAT TCGATGGCAC TACGGGTCTG TCGAGTGCCG AGATGTATGA TCCCAAGACT 
GACATCTGGC GGTTTATCGC CTCCATGTCC ACGCGTCGCA GTTCTGTGGG CGTAGGCGTC 
GTCCACGGAC TTCTTTATGC CGTGGGCGGT TACGATGGCT TCACTCGTCA GTGCTTATCT 
TCAGTGGAGC GCTATAATCC GGACACGGAC ACATGGGTTA ATGTGGCGGA AATGAGTTCG 
CGGAGGAGTG GCGCCGGAGT GGGAGTGCTG AACAATATCC TTTATGCCGT TGGCGGTCAC 
GATGGTCCCA TGGTCAGAAG ATCTGTTGAA GCCTACGATT GTGAAACGAA CTCCTGGCGT 
TCAGTGGCAG ACATGTCATA TTGCCGGCGA AATGCGGGAG TGGTGGCTCA TGATGGCCTG 
CTGTATGTGG TCGGTGGCGA TGATGGCACC TCAAATCTAG CTTCTGTGGA GGTCTACTGC 
CCGGACTCGG ACAGTTGGAG AATACTGCCT GCTTTGATGA CCATCGGACG CAGCTATGCG 
GGCGTGTGTA TGATCGACAA GCCCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724095.1
CDS	153..2222
Translation	MIALSALLTKYTIGIMSNLSNGNSNNNNQQQQQQQQGQNPQQPAQNEGGAGAEFVAPPPGLGAAVGVAAMQQRNRLLQQQQQQHHHHQNPAAEGSGLERGSCLLRYASQNSLDESSQKHVQRPNGKERGTVGQYSNEQHTARSFDAMNEMRKQKQLCDVILVADDVEIHAHRMVLASCSPYFYAMFTSFEESRQARITLQSVDARALELLIDYVYTATVEVNEDNVQVLLTAANLLQLTDVRDACCDFLQTQLDASNCLGIREFADIHACVELLNYAETYIEQHFNEVIQFDEFLNLSHEQVISLIGNDRISVPNEERVYECVIAWLRYDVPMREQFTSLLMEHVRLPFLSKEYITQRVDKEILLEGNIVCKNLIIEALTYHLLPTETKSARTVPRKPVGMPKILLVIGGQAPKAIRSVEWYDLREEKWYQAAEMPNRRCRSGLSVLGDKVYAVGGFNGSLRVRTVDVYDPATDQWANCSNMEARRSTLGVAVLNGCIYAVGGFDGTTGLSSAEMYDPKTDIWRFIASMSTRRSSVGVGVVHGLLYAVGGYDGFTRQCLSSVERYNPDTDTWVNVAEMSSRRSGAGVGVLNNILYAVGGHDGPMVRRSVEAYDCETNSWRSVADMSYCRRNAGVVAHDGLLYVVGGDDGTSNLASVEVYCPDSDSWRILPALMTIGRSYAGVCMIDKPM

Target ORF information:

RefSeq Version	NM_165242.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kelch (kel), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165242.2

ORF Insert Sequence:

ATGATAGCTC TGAGTGCGCT GCTAACCAAA TATACGATCG GTATTATGAG CAACCTAAGC 
AATGGCAACA GCAACAACAA CAACCAGCAG CAGCAGCAAC AACAACAGGG TCAGAACCCA 
CAACAACCAG CACAGAATGA AGGCGGAGCG GGAGCGGAGT TCGTGGCGCC ACCGCCCGGA 
CTAGGTGCCG CAGTGGGCGT GGCAGCCATG CAGCAGCGCA ACCGCCTGCT GCAGCAGCAA 
CAGCAGCAGC ACCACCACCA TCAGAATCCC GCCGCCGAGG GAAGTGGCCT GGAGCGGGGC 
AGCTGCCTTC TGCGCTACGC CAGCCAGAAC TCCCTGGACG AGAGCTCCCA GAAGCACGTC 
CAGCGGCCAA ATGGCAAGGA GCGCGGCACT GTGGGGCAGT ACAGCAATGA ACAGCATACG 
GCGCGCTCCT TCGACGCCAT GAACGAGATG AGAAAACAAA AACAGCTCTG TGATGTGATA 
TTGGTGGCCG ACGATGTGGA GATCCACGCT CACCGCATGG TGCTGGCCTC CTGCAGTCCG 
TACTTCTATG CGATGTTCAC CAGTTTCGAG GAGTCGCGGC AGGCGAGGAT CACCCTCCAG 
AGTGTCGATG CTCGGGCCTT GGAGCTGCTC ATCGACTACG TATACACTGC CACGGTGGAG 
GTAAACGAGG ATAACGTGCA AGTCCTCTTG ACCGCCGCCA ACCTGCTCCA GCTCACGGAT 
GTGCGAGATG CCTGCTGCGA CTTCCTGCAA ACTCAGTTGG ATGCCAGTAA TTGCCTCGGC 
ATCCGGGAGT TTGCCGATAT TCATGCCTGC GTGGAACTTC TCAATTACGC AGAGACCTAC 
ATCGAGCAGC ATTTCAACGA GGTGATCCAG TTCGATGAGT TTCTGAACCT CTCGCACGAG 
CAGGTCATCA GCCTGATTGG CAACGACCGG ATTTCGGTTC CAAATGAGGA GCGCGTATAC 
GAGTGCGTCA TCGCCTGGCT GCGCTACGAT GTTCCTATGC GGGAACAGTT CACCTCGTTG 
CTCATGGAGC ATGTGCGTCT GCCGTTCCTG TCCAAGGAGT ACATCACACA ACGTGTGGAC 
AAGGAGATCC TCCTCGAGGG CAATATCGTG TGCAAGAATC TCATCATTGA GGCATTGACC 
TATCATCTAC TGCCCACTGA GACAAAGTCC GCTAGAACGG TGCCCAGGAA GCCGGTGGGC 
ATGCCCAAGA TACTGCTCGT TATTGGAGGA CAAGCACCCA AGGCTATACG ATCGGTGGAG 
TGGTATGATC TGCGAGAGGA AAAGTGGTAC CAGGCCGCCG AAATGCCCAA CCGCAGATGC 
CGCTCCGGCT TAAGTGTCCT GGGTGACAAA GTCTATGCTG TCGGAGGTTT CAATGGATCA 
CTGCGTGTGC GCACCGTAGA TGTCTACGAT CCTGCCACGG ATCAGTGGGC AAACTGCAGC 
AACATGGAGG CCAGACGATC TACCCTGGGA GTAGCTGTCC TCAATGGTTG CATATACGCC 
GTGGGTGGAT TCGATGGCAC TACGGGTCTG TCGAGTGCCG AGATGTATGA TCCCAAGACT 
GACATCTGGC GGTTTATCGC CTCCATGTCC ACGCGTCGCA GTTCTGTGGG CGTAGGCGTC 
GTCCACGGAC TTCTTTATGC CGTGGGCGGT TACGATGGCT TCACTCGTCA GTGCTTATCT 
TCAGTGGAGC GCTATAATCC GGACACGGAC ACATGGGTTA ATGTGGCGGA AATGAGTTCG 
CGGAGGAGTG GCGCCGGAGT GGGAGTGCTG AACAATATCC TTTATGCCGT TGGCGGTCAC 
GATGGTCCCA TGGTCAGAAG ATCTGTTGAA GCCTACGATT GTGAAACGAA CTCCTGGCGT 
TCAGTGGCAG ACATGTCATA TTGCCGGCGA AATGCGGGAG TGGTGGCTCA TGATGGCCTG 
CTGTATGTGG TCGGTGGCGA TGATGGCACC TCAAATCTAG CTTCTGTGGA GGTCTACTGC 
CCGGACTCGG ACAGTTGGAG AATACTGCCT GCTTTGATGA CCATCGGACG CAGCTATGCG 
GGCGTGTGTA TGATCGACAA GCCCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kel ( NM_057241.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476589.4 Drosophila melanogaster kelch (kel), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14874
Clone ID Related Accession (Same CDS sequence)	NM_057241.3
Accession Version	NM_057241.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kelch, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057241.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATAGCTC TGAGTGCGCT GCTAACCAAA TATACGATCG GTATTATGAG CAACCTAAGC 
AATGGCAACA GCAACAACAA CAACCAGCAG CAGCAGCAAC AACAACAGGG TCAGAACCCA 
CAACAACCAG CACAGAATGA AGGCGGAGCG GGAGCGGAGT TCGTGGCGCC ACCGCCCGGA 
CTAGGTGCCG CAGTGGGCGT GGCAGCCATG CAGCAGCGCA ACCGCCTGCT GCAGCAGCAA 
CAGCAGCAGC ACCACCACCA TCAGAATCCC GCCGCCGAGG GAAGTGGCCT GGAGCGGGGC 
AGCTGCCTTC TGCGCTACGC CAGCCAGAAC TCCCTGGACG AGAGCTCCCA GAAGCACGTC 
CAGCGGCCAA ATGGCAAGGA GCGCGGCACT GTGGGGCAGT ACAGCAATGA ACAGCATACG 
GCGCGCTCCT TCGACGCCAT GAACGAGATG AGAAAACAAA AACAGCTCTG TGATGTGATA 
TTGGTGGCCG ACGATGTGGA GATCCACGCT CACCGCATGG TGCTGGCCTC CTGCAGTCCG 
TACTTCTATG CGATGTTCAC CAGTTTCGAG GAGTCGCGGC AGGCGAGGAT CACCCTCCAG 
AGTGTCGATG CTCGGGCCTT GGAGCTGCTC ATCGACTACG TATACACTGC CACGGTGGAG 
GTAAACGAGG ATAACGTGCA AGTCCTCTTG ACCGCCGCCA ACCTGCTCCA GCTCACGGAT 
GTGCGAGATG CCTGCTGCGA CTTCCTGCAA ACTCAGTTGG ATGCCAGTAA TTGCCTCGGC 
ATCCGGGAGT TTGCCGATAT TCATGCCTGC GTGGAACTTC TCAATTACGC AGAGACCTAC 
ATCGAGCAGC ATTTCAACGA GGTGATCCAG TTCGATGAGT TTCTGAACCT CTCGCACGAG 
CAGGTCATCA GCCTGATTGG CAACGACCGG ATTTCGGTTC CAAATGAGGA GCGCGTATAC 
GAGTGCGTCA TCGCCTGGCT GCGCTACGAT GTTCCTATGC GGGAACAGTT CACCTCGTTG 
CTCATGGAGC ATGTGCGTCT GCCGTTCCTG TCCAAGGAGT ACATCACACA ACGTGTGGAC 
AAGGAGATCC TCCTCGAGGG CAATATCGTG TGCAAGAATC TCATCATTGA GGCATTGACC 
TATCATCTAC TGCCCACTGA GACAAAGTCC GCTAGAACGG TGCCCAGGAA GCCGGTGGGC 
ATGCCCAAGA TACTGCTCGT TATTGGAGGA CAAGCACCCA AGGCTATACG ATCGGTGGAG 
TGGTATGATC TGCGAGAGGA AAAGTGGTAC CAGGCCGCCG AAATGCCCAA CCGCAGATGC 
CGCTCCGGCT TAAGTGTCCT GGGTGACAAA GTCTATGCTG TCGGAGGTTT CAATGGATCA 
CTGCGTGTGC GCACCGTAGA TGTCTACGAT CCTGCCACGG ATCAGTGGGC AAACTGCAGC 
AACATGGAGG CCAGACGATC TACCCTGGGA GTAGCTGTCC TCAATGGTTG CATATACGCC 
GTGGGTGGAT TCGATGGCAC TACGGGTCTG TCGAGTGCCG AGATGTATGA TCCCAAGACT 
GACATCTGGC GGTTTATCGC CTCCATGTCC ACGCGTCGCA GTTCTGTGGG CGTAGGCGTC 
GTCCACGGAC TTCTTTATGC CGTGGGCGGT TACGATGGCT TCACTCGTCA GTGCTTATCT 
TCAGTGGAGC GCTATAATCC GGACACGGAC ACATGGGTTA ATGTGGCGGA AATGAGTTCG 
CGGAGGAGTG GCGCCGGAGT GGGAGTGCTG AACAATATCC TTTATGCCGT TGGCGGTCAC 
GATGGTCCCA TGGTCAGAAG ATCTGTTGAA GCCTACGATT GTGAAACGAA CTCCTGGCGT 
TCAGTGGCAG ACATGTCATA TTGCCGGCGA AATGCGGGAG TGGTGGCTCA TGATGGCCTG 
CTGTATGTGG TCGGTGGCGA TGATGGCACC TCAAATCTAG CTTCTGTGGA GGTCTACTGC 
CCGGACTCGG ACAGTTGGAG AATACTGCCT GCTTTGATGA CCATCGGACG CAGCTATGCG 
GGCGTGTGTA TGATCGACAA GCCCATGTGA ATGGAAGAGC AGGGTGCATT GGCCCGCCAA 
GCTGCTTCGT TGGCCATCGC ACTGCTGGAT GATGAGAATA GCCAGGCGGA GGGCACGATG 
GAGGGCGCCA TTGGTGGTGC CATCTACGGT AATCTGGCGC CGGCTGGTGG GGCTGCAGCG 
GCTGCAGCTC CAGCTGCGCC GGCTCAAGCT CCCCAGCCAA ATCATCCGCA CTACGAGAAC 
ATCTACGCGC CAATTGGACA GCCCAGCAAC AACAACAATA ACAGCGGCAG CAACAGCAAC 
CAAGCTGCAG CCATCGCCAA TGCCAATGCC CCTGCAAATG CCGAGGAAAT TCAGCAACAA 
CAACAACCAG CTCCCACTGA ACCTAATGCC AACAATAATC CGCAACCGCC AACAGCAGCA 
GCTCCAGCAC CATCACAACA ACAACAACAA CAGCAAGCAC AGCCACAACA ACCTCAACGC 
ATACTGCCCA TGAATAACTA TAGGAATGAT TTGTATGATC GATCTGCCGC CGGCGGAGTC 
TGCTCCGCCT ATGATGTTCC GAGAGCCGTG AGATCTGGCC TTGGTTACAG ACGCAACTTT 
AGGATTGATA TGCAGAATGG AAATCGATGT GGAAGCGGTC TTCGTTGTAC TCCACTTTAT 
ACGAACAGTC GATCCAATTG TCAACGCCAG CGCAGTTTTG ACGACACTGA GTCCACGGAC 
GGCTACAATT TGCCTTACGC CGGAGCGGGA ACAATGCGAT ACGAGAACAT CTATGAGCAG 
ATTCGAGATG AGCCACTTTA CCGCACATCC GCTGCGAATC GAGTTCCGCT CTATACACGT 
CTCGATGTTT TGGGTCATGG AATCGGAAGA ATTGAAAGGC ACCTTAGCTC GTCATGCGGC 
AACATCGATC ACTATAACTT GGGCGGACAT TACGCGGTAT TGGGACACTC GCACTTCGGA 
ACCGTGGGAC ACATTCGATT GAACGCCAAT GGATCGGGAG TAGCAGCTCC TGGCGTAGCT 
GGGACCGGCA CTTGCAATGT TCCAAATTGT CAAGGTTATA TGACAGCAGC TGGATCCACA 
GTTCCGGTGG AGTATGCAAA TGTTAAGGTT CCTGTGAAAA ACAGTGCCTC CAGTTTCTTC 
AGCTGCCTGC ACGGTGAGAA CTCGCAGAGT ATGACCAATA TTTACAAAAC GTCTGGAACG 
GCAGCTGCCA TGGCTGCCCA TAATTCCCCA TTGACGCCAA ATGTGTCCAT GGAGCGAGCA 
TCCCGATCGG CGTCCGCGGG GGCAGCTGGA TCAGCAGCAG CAGCTGTTGA AGAGCATTCT 
GCGGCAGATA GTATTCCAAG CTCATCCAAC ATTAATGCCA ATCGGACAAC AGGAGCCATT 
CCGAAAGTGA AAACAGCTAA CAAACCCGCT AAGGAGTCGG GAGGATCCTC CACGGCCGCA 
TCGCCAATTT TGGATAAAAC TACATCCACT GGATCGGGAA AGAGCGTAAC CTTGGCGAAG 
AAAACTTCTA CAGCAGCAGC ACGCTCGAGC TCATCTGGAG ATACCAATGG CAATGGGACT 
TTGAATCGTA TCTCCAAGTC CAGTTTGCAA TGGCTACTAG TAAACAAATG GCTGCCGCTG 
TGGATTGGCC AGGGACCCGA TTGCAAGGTA ATTGACTTCA ACTTCATGTT TTCCCGTGAC 
TGCGTGAGTT GCGATACAGC TTCAGTGGCA TCTCAGATGT CAAATCCGTA TGGCACACCC 
CGTTTGAGTG GTCTTCCCCA GGATATGGTA CGCTTCCAGA GTTCCTGCGC TGGAGCTTGT 
GCGGCAGCCG GAGCAGCTTC TACAATACGA AGAGACGCAA ATGCATCAGC TCGCCCACTG 
CACAGCACTT TGAGTCGCCT GAGGAACGGT GAAAAGAGAA ACCCCAATAG GGTGGCTGGT 
AACTATCAGT ACGAGGATCC TTCCTACGAA AATGTGCACG TTCAGTGGCA GAATGGTTTT 
GAATTCGGAA GATCCCGCGA CTACGATCCC AATTCCACCT ATCATCAGCA GCGCCCTCTG 
CTGCAACGGG CTAGATCGGA GAGTCCCACC TTCAGCAATC AGCAGAGGCG ACTTCAGCGG 
CAAGGAGCTC AAGCTCAACA GCAATCGCAG CAGCCTAAGC CTCCTGGGTC GCCAGATCCC 
TACAAGAACT ACAAACTTAA TGCTGATAAC AACACCTTCA AGCCTAAGCC TATCGCTGCC 
GATGAACTCG AGGGAGCTGT TGGTGGAGCT GTAGCTGAAA TAGCGCTTCC TGAAGTGGAT 
ATCGAAGTTG TAGATCCTGT CAGCTTAAGT GATAACGAGA CTGAGACAAC CAGCAGTCAA 
AACAACCTTC CAAGCACTAC CAACAGCAAT AATCTGAACG AGCACAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476589.4
CDS	153..4586
Translation	MIALSALLTKYTIGIMSNLSNGNSNNNNQQQQQQQQGQNPQQPAQNEGGAGAEFVAPPPGLGAAVGVAAMQQRNRLLQQQQQQHHHHQNPAAEGSGLERGSCLLRYASQNSLDESSQKHVQRPNGKERGTVGQYSNEQHTARSFDAMNEMRKQKQLCDVILVADDVEIHAHRMVLASCSPYFYAMFTSFEESRQARITLQSVDARALELLIDYVYTATVEVNEDNVQVLLTAANLLQLTDVRDACCDFLQTQLDASNCLGIREFADIHACVELLNYAETYIEQHFNEVIQFDEFLNLSHEQVISLIGNDRISVPNEERVYECVIAWLRYDVPMREQFTSLLMEHVRLPFLSKEYITQRVDKEILLEGNIVCKNLIIEALTYHLLPTETKSARTVPRKPVGMPKILLVIGGQAPKAIRSVEWYDLREEKWYQAAEMPNRRCRSGLSVLGDKVYAVGGFNGSLRVRTVDVYDPATDQWANCSNMEARRSTLGVAVLNGCIYAVGGFDGTTGLSSAEMYDPKTDIWRFIASMSTRRSSVGVGVVHGLLYAVGGYDGFTRQCLSSVERYNPDTDTWVNVAEMSSRRSGAGVGVLNNILYAVGGHDGPMVRRSVEAYDCETNSWRSVADMSYCRRNAGVVAHDGLLYVVGGDDGTSNLASVEVYCPDSDSWRILPALMTIGRSYAGVCMIDKPM*MEEQGALARQAASLAIALLDDENSQAEGTMEGAIGGAIYGNLAPAGGAAAAAAPAAPAQAPQPNHPHYENIYAPIGQPSNNNNNSGSNSNQAAAIANANAPANAEEIQQQQQPAPTEPNANNNPQPPTAAAPAPSQQQQQQQAQPQQPQRILPMNNYRNDLYDRSAAGGVCSAYDVPRAVRSGLGYRRNFRIDMQNGNRCGSGLRCTPLYTNSRSNCQRQRSFDDTESTDGYNLPYAGAGTMRYENIYEQIRDEPLYRTSAANRVPLYTRLDVLGHGIGRIERHLSSSCGNIDHYNLGGHYAVLGHSHFGTVGHIRLNANGSGVAAPGVAGTGTCNVPNCQGYMTAAGSTVPVEYANVKVPVKNSASSFFSCLHGENSQSMTNIYKTSGTAAAMAAHNSPLTPNVSMERASRSASAGAAGSAAAAVEEHSAADSIPSSSNINANRTTGAIPKVKTANKPAKESGGSSTAASPILDKTTSTGSGKSVTLAKKTSTAAARSSSSGDTNGNGTLNRISKSSLQWLLVNKWLPLWIGQGPDCKVIDFNFMFSRDCVSCDTASVASQMSNPYGTPRLSGLPQDMVRFQSSCAGACAAAGAASTIRRDANASARPLHSTLSRLRNGEKRNPNRVAGNYQYEDPSYENVHVQWQNGFEFGRSRDYDPNSTYHQQRPLLQRARSESPTFSNQQRRLQRQGAQAQQQSQQPKPPGSPDPYKNYKLNADNNTFKPKPIAADELEGAVGGAVAEIALPEVDIEVVDPVSLSDNETETTSSQNNLPSTTNSNNLNEHND

Target ORF information:

RefSeq Version	NM_057241.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kelch (kel), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057241.3

ORF Insert Sequence:

ATGATAGCTC TGAGTGCGCT GCTAACCAAA TATACGATCG GTATTATGAG CAACCTAAGC 
AATGGCAACA GCAACAACAA CAACCAGCAG CAGCAGCAAC AACAACAGGG TCAGAACCCA 
CAACAACCAG CACAGAATGA AGGCGGAGCG GGAGCGGAGT TCGTGGCGCC ACCGCCCGGA 
CTAGGTGCCG CAGTGGGCGT GGCAGCCATG CAGCAGCGCA ACCGCCTGCT GCAGCAGCAA 
CAGCAGCAGC ACCACCACCA TCAGAATCCC GCCGCCGAGG GAAGTGGCCT GGAGCGGGGC 
AGCTGCCTTC TGCGCTACGC CAGCCAGAAC TCCCTGGACG AGAGCTCCCA GAAGCACGTC 
CAGCGGCCAA ATGGCAAGGA GCGCGGCACT GTGGGGCAGT ACAGCAATGA ACAGCATACG 
GCGCGCTCCT TCGACGCCAT GAACGAGATG AGAAAACAAA AACAGCTCTG TGATGTGATA 
TTGGTGGCCG ACGATGTGGA GATCCACGCT CACCGCATGG TGCTGGCCTC CTGCAGTCCG 
TACTTCTATG CGATGTTCAC CAGTTTCGAG GAGTCGCGGC AGGCGAGGAT CACCCTCCAG 
AGTGTCGATG CTCGGGCCTT GGAGCTGCTC ATCGACTACG TATACACTGC CACGGTGGAG 
GTAAACGAGG ATAACGTGCA AGTCCTCTTG ACCGCCGCCA ACCTGCTCCA GCTCACGGAT 
GTGCGAGATG CCTGCTGCGA CTTCCTGCAA ACTCAGTTGG ATGCCAGTAA TTGCCTCGGC 
ATCCGGGAGT TTGCCGATAT TCATGCCTGC GTGGAACTTC TCAATTACGC AGAGACCTAC 
ATCGAGCAGC ATTTCAACGA GGTGATCCAG TTCGATGAGT TTCTGAACCT CTCGCACGAG 
CAGGTCATCA GCCTGATTGG CAACGACCGG ATTTCGGTTC CAAATGAGGA GCGCGTATAC 
GAGTGCGTCA TCGCCTGGCT GCGCTACGAT GTTCCTATGC GGGAACAGTT CACCTCGTTG 
CTCATGGAGC ATGTGCGTCT GCCGTTCCTG TCCAAGGAGT ACATCACACA ACGTGTGGAC 
AAGGAGATCC TCCTCGAGGG CAATATCGTG TGCAAGAATC TCATCATTGA GGCATTGACC 
TATCATCTAC TGCCCACTGA GACAAAGTCC GCTAGAACGG TGCCCAGGAA GCCGGTGGGC 
ATGCCCAAGA TACTGCTCGT TATTGGAGGA CAAGCACCCA AGGCTATACG ATCGGTGGAG 
TGGTATGATC TGCGAGAGGA AAAGTGGTAC CAGGCCGCCG AAATGCCCAA CCGCAGATGC 
CGCTCCGGCT TAAGTGTCCT GGGTGACAAA GTCTATGCTG TCGGAGGTTT CAATGGATCA 
CTGCGTGTGC GCACCGTAGA TGTCTACGAT CCTGCCACGG ATCAGTGGGC AAACTGCAGC 
AACATGGAGG CCAGACGATC TACCCTGGGA GTAGCTGTCC TCAATGGTTG CATATACGCC 
GTGGGTGGAT TCGATGGCAC TACGGGTCTG TCGAGTGCCG AGATGTATGA TCCCAAGACT 
GACATCTGGC GGTTTATCGC CTCCATGTCC ACGCGTCGCA GTTCTGTGGG CGTAGGCGTC 
GTCCACGGAC TTCTTTATGC CGTGGGCGGT TACGATGGCT TCACTCGTCA GTGCTTATCT 
TCAGTGGAGC GCTATAATCC GGACACGGAC ACATGGGTTA ATGTGGCGGA AATGAGTTCG 
CGGAGGAGTG GCGCCGGAGT GGGAGTGCTG AACAATATCC TTTATGCCGT TGGCGGTCAC 
GATGGTCCCA TGGTCAGAAG ATCTGTTGAA GCCTACGATT GTGAAACGAA CTCCTGGCGT 
TCAGTGGCAG ACATGTCATA TTGCCGGCGA AATGCGGGAG TGGTGGCTCA TGATGGCCTG 
CTGTATGTGG TCGGTGGCGA TGATGGCACC TCAAATCTAG CTTCTGTGGA GGTCTACTGC 
CCGGACTCGG ACAGTTGGAG AATACTGCCT GCTTTGATGA CCATCGGACG CAGCTATGCG 
GGCGTGTGTA TGATCGACAA GCCCATGTGA ATGGAAGAGC AGGGTGCATT GGCCCGCCAA 
GCTGCTTCGT TGGCCATCGC ACTGCTGGAT GATGAGAATA GCCAGGCGGA GGGCACGATG 
GAGGGCGCCA TTGGTGGTGC CATCTACGGT AATCTGGCGC CGGCTGGTGG GGCTGCAGCG 
GCTGCAGCTC CAGCTGCGCC GGCTCAAGCT CCCCAGCCAA ATCATCCGCA CTACGAGAAC 
ATCTACGCGC CAATTGGACA GCCCAGCAAC AACAACAATA ACAGCGGCAG CAACAGCAAC 
CAAGCTGCAG CCATCGCCAA TGCCAATGCC CCTGCAAATG CCGAGGAAAT TCAGCAACAA 
CAACAACCAG CTCCCACTGA ACCTAATGCC AACAATAATC CGCAACCGCC AACAGCAGCA 
GCTCCAGCAC CATCACAACA ACAACAACAA CAGCAAGCAC AGCCACAACA ACCTCAACGC 
ATACTGCCCA TGAATAACTA TAGGAATGAT TTGTATGATC GATCTGCCGC CGGCGGAGTC 
TGCTCCGCCT ATGATGTTCC GAGAGCCGTG AGATCTGGCC TTGGTTACAG ACGCAACTTT 
AGGATTGATA TGCAGAATGG AAATCGATGT GGAAGCGGTC TTCGTTGTAC TCCACTTTAT 
ACGAACAGTC GATCCAATTG TCAACGCCAG CGCAGTTTTG ACGACACTGA GTCCACGGAC 
GGCTACAATT TGCCTTACGC CGGAGCGGGA ACAATGCGAT ACGAGAACAT CTATGAGCAG 
ATTCGAGATG AGCCACTTTA CCGCACATCC GCTGCGAATC GAGTTCCGCT CTATACACGT 
CTCGATGTTT TGGGTCATGG AATCGGAAGA ATTGAAAGGC ACCTTAGCTC GTCATGCGGC 
AACATCGATC ACTATAACTT GGGCGGACAT TACGCGGTAT TGGGACACTC GCACTTCGGA 
ACCGTGGGAC ACATTCGATT GAACGCCAAT GGATCGGGAG TAGCAGCTCC TGGCGTAGCT 
GGGACCGGCA CTTGCAATGT TCCAAATTGT CAAGGTTATA TGACAGCAGC TGGATCCACA 
GTTCCGGTGG AGTATGCAAA TGTTAAGGTT CCTGTGAAAA ACAGTGCCTC CAGTTTCTTC 
AGCTGCCTGC ACGGTGAGAA CTCGCAGAGT ATGACCAATA TTTACAAAAC GTCTGGAACG 
GCAGCTGCCA TGGCTGCCCA TAATTCCCCA TTGACGCCAA ATGTGTCCAT GGAGCGAGCA 
TCCCGATCGG CGTCCGCGGG GGCAGCTGGA TCAGCAGCAG CAGCTGTTGA AGAGCATTCT 
GCGGCAGATA GTATTCCAAG CTCATCCAAC ATTAATGCCA ATCGGACAAC AGGAGCCATT 
CCGAAAGTGA AAACAGCTAA CAAACCCGCT AAGGAGTCGG GAGGATCCTC CACGGCCGCA 
TCGCCAATTT TGGATAAAAC TACATCCACT GGATCGGGAA AGAGCGTAAC CTTGGCGAAG 
AAAACTTCTA CAGCAGCAGC ACGCTCGAGC TCATCTGGAG ATACCAATGG CAATGGGACT 
TTGAATCGTA TCTCCAAGTC CAGTTTGCAA TGGCTACTAG TAAACAAATG GCTGCCGCTG 
TGGATTGGCC AGGGACCCGA TTGCAAGGTA ATTGACTTCA ACTTCATGTT TTCCCGTGAC 
TGCGTGAGTT GCGATACAGC TTCAGTGGCA TCTCAGATGT CAAATCCGTA TGGCACACCC 
CGTTTGAGTG GTCTTCCCCA GGATATGGTA CGCTTCCAGA GTTCCTGCGC TGGAGCTTGT 
GCGGCAGCCG GAGCAGCTTC TACAATACGA AGAGACGCAA ATGCATCAGC TCGCCCACTG 
CACAGCACTT TGAGTCGCCT GAGGAACGGT GAAAAGAGAA ACCCCAATAG GGTGGCTGGT 
AACTATCAGT ACGAGGATCC TTCCTACGAA AATGTGCACG TTCAGTGGCA GAATGGTTTT 
GAATTCGGAA GATCCCGCGA CTACGATCCC AATTCCACCT ATCATCAGCA GCGCCCTCTG 
CTGCAACGGG CTAGATCGGA GAGTCCCACC TTCAGCAATC AGCAGAGGCG ACTTCAGCGG 
CAAGGAGCTC AAGCTCAACA GCAATCGCAG CAGCCTAAGC CTCCTGGGTC GCCAGATCCC 
TACAAGAACT ACAAACTTAA TGCTGATAAC AACACCTTCA AGCCTAAGCC TATCGCTGCC 
GATGAACTCG AGGGAGCTGT TGGTGGAGCT GTAGCTGAAA TAGCGCTTCC TGAAGTGGAT 
ATCGAAGTTG TAGATCCTGT CAGCTTAAGT GATAACGAGA CTGAGACAAC CAGCAGTCAA 
AACAACCTTC CAAGCACTAC CAACAGCAAT AATCTGAACG AGCACAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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kel cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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