grp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	grp
Entrez Gene ID	34993
Full Name	grapes
Synonyms	1C,CG17161,CHK1,CHK1/Grapes,Chk1,Dchk1,DmChk1,Dmel\CG17161,Grp,Grp/DChk1,Pk36A,Pk?1,chk1,dChk1,grp(chk),grps,l(2)k15102,lemp
General protein information	Preferred Names grapes Names CG17161 gene product from transcript CG17161-RC CG17161-PA CG17161-PB CG17161-PC CG17161-PD CG17161-PF Chk1/Grapes checkpoint kinase 1 grp-PA grp-PB grp-PC grp-PD grp-PF last empress lethal (2) k15102 protein kinase-like 36A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 36A10-36A10

mRNA and Protein(s)

mRNA	Protein	Name
NM_165172.3	NP_723986.1	grapes, isoform C
NM_165173.3	NP_723987.2	grapes, isoform D
NM_165171.3	NP_723985.1	grapes, isoform B
NM_057663.4	NP_477011.1	grapes, isoform A
NM_001273579.1	NP_001260508.1	grapes, isoform F

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CHEK1	NP_001091492.1
Gallus gallus (chicken)	STT3A	NP_989676.1
Saccharomyces cerevisiae (baker's yeast)	CHK1	NP_009833.1
Eremothecium gossypii	AGOS_AEL185C	NP_984676.1
Magnaporthe oryzae (rice blast fungus)	MGG_03729	XP_003716151.1
Xenopus tropicalis (tropical clawed frog)	chek1	XP_002937561.2
Homo sapiens (human)	CHEK1	NP_001265.2
Rattus norvegicus (Norway rat)	Chek1	NP_536325.1
Caenorhabditis elegans (roundworm)	chk-1	NP_001024233.1
Kluyveromyces lactis	KLLA0F01276g	XP_455137.1
Schizosaccharomyces pombe (fission yeast)	chk1	NP_588070.1
	LOC713358	XP_001111431.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008118	XP_317338.4
Pan troglodytes (chimpanzee)	CHEK1	XP_001146296.1
Mus musculus (house mouse)	Chek1	NP_031717.2
Neurospora crassa	NCU08346	XP_965249.2
Canis lupus familiaris (dog)	CHEK1	XP_852191.1
Danio rerio (zebrafish)	chek1	NP_956487.1
Drosophila melanogaster (fruit fly)	grp	NP_723986.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Grp was assumed to play a direct role in the immune response in D. melanogaster.
Title: [Functional analysis of Grp and Iris, the gag and env domesticated errantivirus genes, in the Drosophila melanogaster genome].

The Grp gene is expressed at the adult stage of D. melanogaster in gender-specific and tissue-specific manner.
Title: Domesticated retroviral GAG gene in Drosophila: new functions for an old gene.

Relationships between DNA replication, chromosome condensation, and anaphase entry are mediated by the cell cycle kinases Grp (Chk1) and dWee1 (Wee1).
Title: Chk1 and Wee1 kinases coordinate DNA replication, chromosome condensation, and anaphase entry.

The Grp gene has been demonstrated to be a functional gene in the D. melanogaster genome, bit its function is yet to be determined.
Title: [Translational analysis of the Grp gene, a genomic homologue of the Gag gene of the gypsy retrotransposon of Drosophila melanogaster].

the Grp gene is not a pseudogene, but it is a functional gene of the D. melanogaster genome whose role remains to be elucidated.
Title: [Transcriptional analysis of the Grp gene, a genomic homolog of the retrotransposon gypsy gag gene, in Drosophila melanogaster].

The following grp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the grp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14760	NM_165172.3 Latest version!	Drosophila melanogaster grapes (grp), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14760	NM_165171.3 Latest version!	Drosophila melanogaster grapes (grp), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14760	NM_057663.4 Latest version!	Drosophila melanogaster grapes (grp), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14761	NM_001273579.1 Latest version!	Drosophila melanogaster grapes (grp), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14761	NM_165173.3 Latest version!	Drosophila melanogaster grapes (grp), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

grp ( NM_165172.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723986.1 Drosophila melanogaster grapes (grp), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa14760
Clone ID Related Accession (Same CDS sequence)	NM_165172.3 , NM_165171.3 , NM_057663.4
Accession Version	NM_165172.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grapes, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165172.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCAA CGCTGACGGA AGCGGGAACA GGTCCTGCGG CCACCAGGGA GTTCGTCGAG 
GGATGGACTT TGGCCCAAAC TCTGGGCGAA GGTGCCTACG GCGAGGTAAA GCTACTAATC 
AACCGGCAGA CTGGCGAGGC TGTGGCCATG AAAATGGTGG ATCTAAAAAA ACATCCGGAT 
GCAGCGAACT CGGTGCGAAA GGAGGTATGC ATACAGAAGA TGCTCCAGGA TAAGCATATC 
CTCCGATTTT TCGGCAAACG TTCGCAAGGC AGTGTGGAGT ACATATTCCT GGAATACGCC 
GCCGGCGGAG AGCTATTCGA TCGAATAGAA CCAGACGTGG GAATGCCGCA GCATGAGGCT 
CAAAGGTATT TTACACAGCT CCTGTCCGGA CTCAATTACC TGCATCAGCG TGGGATCGCT 
CATCGGGATC TGAAGCCGGA AAATCTGCTG CTTGACGAGC ATGACAACGT GAAAATATCG 
GACTTTGGCA TGGCTACTAT GTTTAGGTGC AAGGGCAAGG AGCGACTGCT GGACAAACGC 
TGCGGCACCT TGCCGTATGT GGCGCCCGAG GTGCTACAGA AGGCATATCA CGCCCAGCCG 
GCGGATCTCT GGTCGTGTGG CGTTATATTG GTGACAATGC TGGCGGGTGA GCTGCCCTGG 
GATCAGCCGT CCACCAATTG CACGGAGTTC ACCAACTGGA GGGATAACGA TCACTGGCAA 
CTGCAGACTC CTTGGAGCAA ACTGGACACC TTGGCTATTT CGCTGCTTCG CAAGCTGCTG 
GCCACCAGTC CTGGCACGCG TTTGACCCTG GAGAAAACCC TGGATCACAA ATGGTGCAAC 
ATGCAGTTTG CAGACAATGA ACGTTCCTAT GACCTGGTGG ACTCGGCGGC TGCCCTGGAG 
ATATGCTCGC CAAAGGCTAA GAGGCAGCGT CTGCAGTCTA GTGCCCACTT GAGCAATGGC 
CTGGATGACT CCATCTCCCG GAACTACTGC TCTCAACCCA TGCCCACAAT GCGCAGCGAC 
GATGACTTCA ATGTCAGACT GGGCAGTGGC CGATCCAAGG AGGATGGAGG CGACCGCCAG 
ACGTTGGCCC AGGAGGCTCG GCTCAGTTAC TCCTTCTCGC AACCAGCTTT GCTTGATGAT 
CTCCTACTGG CTACCCAGAT GAACCAAACG CAGAACGCTT CCCAGAACTA TTTCCAGCGT 
TTGGTGAGGA GAATGACCCG ATTCTTTGTG ACCACACGAT GGGATGACAC TATCAAGCGA 
TTGGTGGGAA CCATCGAAAG ACTGGGTGGT TATACGTGCA AATTTGGTGA CGACGGAGTG 
GTCACCGTAT CCACAGTCGA TCGGAATAAG CTGCGATTGG TTTTCAAGGC ACACATTATA 
GAGATGGATG GCAAGATTCT TGTTGACTGC CGGCTGTCAA AGGGTTGCGG CTTGGAGTTT 
AAACGACGAT TTATCAAGAT CAAAAACGCC CTGGAGGATA TCGTGCTTAA AGGACCCACC 
ACCTGGCCGA TTGCGATTGC TACAAATTCG GTGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723986.1
CDS	521..2059
Translation	MAATLTEAGTGPAATREFVEGWTLAQTLGEGAYGEVKLLINRQTGEAVAMKMVDLKKHPDAANSVRKEVCIQKMLQDKHILRFFGKRSQGSVEYIFLEYAAGGELFDRIEPDVGMPQHEAQRYFTQLLSGLNYLHQRGIAHRDLKPENLLLDEHDNVKISDFGMATMFRCKGKERLLDKRCGTLPYVAPEVLQKAYHAQPADLWSCGVILVTMLAGELPWDQPSTNCTEFTNWRDNDHWQLQTPWSKLDTLAISLLRKLLATSPGTRLTLEKTLDHKWCNMQFADNERSYDLVDSAAALEICSPKAKRQRLQSSAHLSNGLDDSISRNYCSQPMPTMRSDDDFNVRLGSGRSKEDGGDRQTLAQEARLSYSFSQPALLDDLLLATQMNQTQNASQNYFQRLVRRMTRFFVTTRWDDTIKRLVGTIERLGGYTCKFGDDGVVTVSTVDRNKLRLVFKAHIIEMDGKILVDCRLSKGCGLEFKRRFIKIKNALEDIVLKGPTTWPIAIATNSVP

Target ORF information:

RefSeq Version	NM_165172.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grapes (grp), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165172.3

ORF Insert Sequence:

ATGGCTGCAA CGCTGACGGA AGCGGGAACA GGTCCTGCGG CCACCAGGGA GTTCGTCGAG 
GGATGGACTT TGGCCCAAAC TCTGGGCGAA GGTGCCTACG GCGAGGTAAA GCTACTAATC 
AACCGGCAGA CTGGCGAGGC TGTGGCCATG AAAATGGTGG ATCTAAAAAA ACATCCGGAT 
GCAGCGAACT CGGTGCGAAA GGAGGTATGC ATACAGAAGA TGCTCCAGGA TAAGCATATC 
CTCCGATTTT TCGGCAAACG TTCGCAAGGC AGTGTGGAGT ACATATTCCT GGAATACGCC 
GCCGGCGGAG AGCTATTCGA TCGAATAGAA CCAGACGTGG GAATGCCGCA GCATGAGGCT 
CAAAGGTATT TTACACAGCT CCTGTCCGGA CTCAATTACC TGCATCAGCG TGGGATCGCT 
CATCGGGATC TGAAGCCGGA AAATCTGCTG CTTGACGAGC ATGACAACGT GAAAATATCG 
GACTTTGGCA TGGCTACTAT GTTTAGGTGC AAGGGCAAGG AGCGACTGCT GGACAAACGC 
TGCGGCACCT TGCCGTATGT GGCGCCCGAG GTGCTACAGA AGGCATATCA CGCCCAGCCG 
GCGGATCTCT GGTCGTGTGG CGTTATATTG GTGACAATGC TGGCGGGTGA GCTGCCCTGG 
GATCAGCCGT CCACCAATTG CACGGAGTTC ACCAACTGGA GGGATAACGA TCACTGGCAA 
CTGCAGACTC CTTGGAGCAA ACTGGACACC TTGGCTATTT CGCTGCTTCG CAAGCTGCTG 
GCCACCAGTC CTGGCACGCG TTTGACCCTG GAGAAAACCC TGGATCACAA ATGGTGCAAC 
ATGCAGTTTG CAGACAATGA ACGTTCCTAT GACCTGGTGG ACTCGGCGGC TGCCCTGGAG 
ATATGCTCGC CAAAGGCTAA GAGGCAGCGT CTGCAGTCTA GTGCCCACTT GAGCAATGGC 
CTGGATGACT CCATCTCCCG GAACTACTGC TCTCAACCCA TGCCCACAAT GCGCAGCGAC 
GATGACTTCA ATGTCAGACT GGGCAGTGGC CGATCCAAGG AGGATGGAGG CGACCGCCAG 
ACGTTGGCCC AGGAGGCTCG GCTCAGTTAC TCCTTCTCGC AACCAGCTTT GCTTGATGAT 
CTCCTACTGG CTACCCAGAT GAACCAAACG CAGAACGCTT CCCAGAACTA TTTCCAGCGT 
TTGGTGAGGA GAATGACCCG ATTCTTTGTG ACCACACGAT GGGATGACAC TATCAAGCGA 
TTGGTGGGAA CCATCGAAAG ACTGGGTGGT TATACGTGCA AATTTGGTGA CGACGGAGTG 
GTCACCGTAT CCACAGTCGA TCGGAATAAG CTGCGATTGG TTTTCAAGGC ACACATTATA 
GAGATGGATG GCAAGATTCT TGTTGACTGC CGGCTGTCAA AGGGTTGCGG CTTGGAGTTT 
AAACGACGAT TTATCAAGAT CAAAAACGCC CTGGAGGATA TCGTGCTTAA AGGACCCACC 
ACCTGGCCGA TTGCGATTGC TACAAATTCG GTGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grp ( NM_165171.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723985.1 Drosophila melanogaster grapes (grp), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14760
Clone ID Related Accession (Same CDS sequence)	NM_165172.3 , NM_165171.3 , NM_057663.4
Accession Version	NM_165171.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grapes, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165171.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCAA CGCTGACGGA AGCGGGAACA GGTCCTGCGG CCACCAGGGA GTTCGTCGAG 
GGATGGACTT TGGCCCAAAC TCTGGGCGAA GGTGCCTACG GCGAGGTAAA GCTACTAATC 
AACCGGCAGA CTGGCGAGGC TGTGGCCATG AAAATGGTGG ATCTAAAAAA ACATCCGGAT 
GCAGCGAACT CGGTGCGAAA GGAGGTATGC ATACAGAAGA TGCTCCAGGA TAAGCATATC 
CTCCGATTTT TCGGCAAACG TTCGCAAGGC AGTGTGGAGT ACATATTCCT GGAATACGCC 
GCCGGCGGAG AGCTATTCGA TCGAATAGAA CCAGACGTGG GAATGCCGCA GCATGAGGCT 
CAAAGGTATT TTACACAGCT CCTGTCCGGA CTCAATTACC TGCATCAGCG TGGGATCGCT 
CATCGGGATC TGAAGCCGGA AAATCTGCTG CTTGACGAGC ATGACAACGT GAAAATATCG 
GACTTTGGCA TGGCTACTAT GTTTAGGTGC AAGGGCAAGG AGCGACTGCT GGACAAACGC 
TGCGGCACCT TGCCGTATGT GGCGCCCGAG GTGCTACAGA AGGCATATCA CGCCCAGCCG 
GCGGATCTCT GGTCGTGTGG CGTTATATTG GTGACAATGC TGGCGGGTGA GCTGCCCTGG 
GATCAGCCGT CCACCAATTG CACGGAGTTC ACCAACTGGA GGGATAACGA TCACTGGCAA 
CTGCAGACTC CTTGGAGCAA ACTGGACACC TTGGCTATTT CGCTGCTTCG CAAGCTGCTG 
GCCACCAGTC CTGGCACGCG TTTGACCCTG GAGAAAACCC TGGATCACAA ATGGTGCAAC 
ATGCAGTTTG CAGACAATGA ACGTTCCTAT GACCTGGTGG ACTCGGCGGC TGCCCTGGAG 
ATATGCTCGC CAAAGGCTAA GAGGCAGCGT CTGCAGTCTA GTGCCCACTT GAGCAATGGC 
CTGGATGACT CCATCTCCCG GAACTACTGC TCTCAACCCA TGCCCACAAT GCGCAGCGAC 
GATGACTTCA ATGTCAGACT GGGCAGTGGC CGATCCAAGG AGGATGGAGG CGACCGCCAG 
ACGTTGGCCC AGGAGGCTCG GCTCAGTTAC TCCTTCTCGC AACCAGCTTT GCTTGATGAT 
CTCCTACTGG CTACCCAGAT GAACCAAACG CAGAACGCTT CCCAGAACTA TTTCCAGCGT 
TTGGTGAGGA GAATGACCCG ATTCTTTGTG ACCACACGAT GGGATGACAC TATCAAGCGA 
TTGGTGGGAA CCATCGAAAG ACTGGGTGGT TATACGTGCA AATTTGGTGA CGACGGAGTG 
GTCACCGTAT CCACAGTCGA TCGGAATAAG CTGCGATTGG TTTTCAAGGC ACACATTATA 
GAGATGGATG GCAAGATTCT TGTTGACTGC CGGCTGTCAA AGGGTTGCGG CTTGGAGTTT 
AAACGACGAT TTATCAAGAT CAAAAACGCC CTGGAGGATA TCGTGCTTAA AGGACCCACC 
ACCTGGCCGA TTGCGATTGC TACAAATTCG GTGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723985.1
CDS	411..1949
Translation	MAATLTEAGTGPAATREFVEGWTLAQTLGEGAYGEVKLLINRQTGEAVAMKMVDLKKHPDAANSVRKEVCIQKMLQDKHILRFFGKRSQGSVEYIFLEYAAGGELFDRIEPDVGMPQHEAQRYFTQLLSGLNYLHQRGIAHRDLKPENLLLDEHDNVKISDFGMATMFRCKGKERLLDKRCGTLPYVAPEVLQKAYHAQPADLWSCGVILVTMLAGELPWDQPSTNCTEFTNWRDNDHWQLQTPWSKLDTLAISLLRKLLATSPGTRLTLEKTLDHKWCNMQFADNERSYDLVDSAAALEICSPKAKRQRLQSSAHLSNGLDDSISRNYCSQPMPTMRSDDDFNVRLGSGRSKEDGGDRQTLAQEARLSYSFSQPALLDDLLLATQMNQTQNASQNYFQRLVRRMTRFFVTTRWDDTIKRLVGTIERLGGYTCKFGDDGVVTVSTVDRNKLRLVFKAHIIEMDGKILVDCRLSKGCGLEFKRRFIKIKNALEDIVLKGPTTWPIAIATNSVP

Target ORF information:

RefSeq Version	NM_165171.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grapes (grp), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165171.3

ORF Insert Sequence:

ATGGCTGCAA CGCTGACGGA AGCGGGAACA GGTCCTGCGG CCACCAGGGA GTTCGTCGAG 
GGATGGACTT TGGCCCAAAC TCTGGGCGAA GGTGCCTACG GCGAGGTAAA GCTACTAATC 
AACCGGCAGA CTGGCGAGGC TGTGGCCATG AAAATGGTGG ATCTAAAAAA ACATCCGGAT 
GCAGCGAACT CGGTGCGAAA GGAGGTATGC ATACAGAAGA TGCTCCAGGA TAAGCATATC 
CTCCGATTTT TCGGCAAACG TTCGCAAGGC AGTGTGGAGT ACATATTCCT GGAATACGCC 
GCCGGCGGAG AGCTATTCGA TCGAATAGAA CCAGACGTGG GAATGCCGCA GCATGAGGCT 
CAAAGGTATT TTACACAGCT CCTGTCCGGA CTCAATTACC TGCATCAGCG TGGGATCGCT 
CATCGGGATC TGAAGCCGGA AAATCTGCTG CTTGACGAGC ATGACAACGT GAAAATATCG 
GACTTTGGCA TGGCTACTAT GTTTAGGTGC AAGGGCAAGG AGCGACTGCT GGACAAACGC 
TGCGGCACCT TGCCGTATGT GGCGCCCGAG GTGCTACAGA AGGCATATCA CGCCCAGCCG 
GCGGATCTCT GGTCGTGTGG CGTTATATTG GTGACAATGC TGGCGGGTGA GCTGCCCTGG 
GATCAGCCGT CCACCAATTG CACGGAGTTC ACCAACTGGA GGGATAACGA TCACTGGCAA 
CTGCAGACTC CTTGGAGCAA ACTGGACACC TTGGCTATTT CGCTGCTTCG CAAGCTGCTG 
GCCACCAGTC CTGGCACGCG TTTGACCCTG GAGAAAACCC TGGATCACAA ATGGTGCAAC 
ATGCAGTTTG CAGACAATGA ACGTTCCTAT GACCTGGTGG ACTCGGCGGC TGCCCTGGAG 
ATATGCTCGC CAAAGGCTAA GAGGCAGCGT CTGCAGTCTA GTGCCCACTT GAGCAATGGC 
CTGGATGACT CCATCTCCCG GAACTACTGC TCTCAACCCA TGCCCACAAT GCGCAGCGAC 
GATGACTTCA ATGTCAGACT GGGCAGTGGC CGATCCAAGG AGGATGGAGG CGACCGCCAG 
ACGTTGGCCC AGGAGGCTCG GCTCAGTTAC TCCTTCTCGC AACCAGCTTT GCTTGATGAT 
CTCCTACTGG CTACCCAGAT GAACCAAACG CAGAACGCTT CCCAGAACTA TTTCCAGCGT 
TTGGTGAGGA GAATGACCCG ATTCTTTGTG ACCACACGAT GGGATGACAC TATCAAGCGA 
TTGGTGGGAA CCATCGAAAG ACTGGGTGGT TATACGTGCA AATTTGGTGA CGACGGAGTG 
GTCACCGTAT CCACAGTCGA TCGGAATAAG CTGCGATTGG TTTTCAAGGC ACACATTATA 
GAGATGGATG GCAAGATTCT TGTTGACTGC CGGCTGTCAA AGGGTTGCGG CTTGGAGTTT 
AAACGACGAT TTATCAAGAT CAAAAACGCC CTGGAGGATA TCGTGCTTAA AGGACCCACC 
ACCTGGCCGA TTGCGATTGC TACAAATTCG GTGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grp ( NM_057663.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477011.1 Drosophila melanogaster grapes (grp), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14760
Clone ID Related Accession (Same CDS sequence)	NM_165172.3 , NM_165171.3 , NM_057663.4
Accession Version	NM_057663.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grapes, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057663.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCAA CGCTGACGGA AGCGGGAACA GGTCCTGCGG CCACCAGGGA GTTCGTCGAG 
GGATGGACTT TGGCCCAAAC TCTGGGCGAA GGTGCCTACG GCGAGGTAAA GCTACTAATC 
AACCGGCAGA CTGGCGAGGC TGTGGCCATG AAAATGGTGG ATCTAAAAAA ACATCCGGAT 
GCAGCGAACT CGGTGCGAAA GGAGGTATGC ATACAGAAGA TGCTCCAGGA TAAGCATATC 
CTCCGATTTT TCGGCAAACG TTCGCAAGGC AGTGTGGAGT ACATATTCCT GGAATACGCC 
GCCGGCGGAG AGCTATTCGA TCGAATAGAA CCAGACGTGG GAATGCCGCA GCATGAGGCT 
CAAAGGTATT TTACACAGCT CCTGTCCGGA CTCAATTACC TGCATCAGCG TGGGATCGCT 
CATCGGGATC TGAAGCCGGA AAATCTGCTG CTTGACGAGC ATGACAACGT GAAAATATCG 
GACTTTGGCA TGGCTACTAT GTTTAGGTGC AAGGGCAAGG AGCGACTGCT GGACAAACGC 
TGCGGCACCT TGCCGTATGT GGCGCCCGAG GTGCTACAGA AGGCATATCA CGCCCAGCCG 
GCGGATCTCT GGTCGTGTGG CGTTATATTG GTGACAATGC TGGCGGGTGA GCTGCCCTGG 
GATCAGCCGT CCACCAATTG CACGGAGTTC ACCAACTGGA GGGATAACGA TCACTGGCAA 
CTGCAGACTC CTTGGAGCAA ACTGGACACC TTGGCTATTT CGCTGCTTCG CAAGCTGCTG 
GCCACCAGTC CTGGCACGCG TTTGACCCTG GAGAAAACCC TGGATCACAA ATGGTGCAAC 
ATGCAGTTTG CAGACAATGA ACGTTCCTAT GACCTGGTGG ACTCGGCGGC TGCCCTGGAG 
ATATGCTCGC CAAAGGCTAA GAGGCAGCGT CTGCAGTCTA GTGCCCACTT GAGCAATGGC 
CTGGATGACT CCATCTCCCG GAACTACTGC TCTCAACCCA TGCCCACAAT GCGCAGCGAC 
GATGACTTCA ATGTCAGACT GGGCAGTGGC CGATCCAAGG AGGATGGAGG CGACCGCCAG 
ACGTTGGCCC AGGAGGCTCG GCTCAGTTAC TCCTTCTCGC AACCAGCTTT GCTTGATGAT 
CTCCTACTGG CTACCCAGAT GAACCAAACG CAGAACGCTT CCCAGAACTA TTTCCAGCGT 
TTGGTGAGGA GAATGACCCG ATTCTTTGTG ACCACACGAT GGGATGACAC TATCAAGCGA 
TTGGTGGGAA CCATCGAAAG ACTGGGTGGT TATACGTGCA AATTTGGTGA CGACGGAGTG 
GTCACCGTAT CCACAGTCGA TCGGAATAAG CTGCGATTGG TTTTCAAGGC ACACATTATA 
GAGATGGATG GCAAGATTCT TGTTGACTGC CGGCTGTCAA AGGGTTGCGG CTTGGAGTTT 
AAACGACGAT TTATCAAGAT CAAAAACGCC CTGGAGGATA TCGTGCTTAA AGGACCCACC 
ACCTGGCCGA TTGCGATTGC TACAAATTCG GTGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477011.1
CDS	740..2278
Translation	MAATLTEAGTGPAATREFVEGWTLAQTLGEGAYGEVKLLINRQTGEAVAMKMVDLKKHPDAANSVRKEVCIQKMLQDKHILRFFGKRSQGSVEYIFLEYAAGGELFDRIEPDVGMPQHEAQRYFTQLLSGLNYLHQRGIAHRDLKPENLLLDEHDNVKISDFGMATMFRCKGKERLLDKRCGTLPYVAPEVLQKAYHAQPADLWSCGVILVTMLAGELPWDQPSTNCTEFTNWRDNDHWQLQTPWSKLDTLAISLLRKLLATSPGTRLTLEKTLDHKWCNMQFADNERSYDLVDSAAALEICSPKAKRQRLQSSAHLSNGLDDSISRNYCSQPMPTMRSDDDFNVRLGSGRSKEDGGDRQTLAQEARLSYSFSQPALLDDLLLATQMNQTQNASQNYFQRLVRRMTRFFVTTRWDDTIKRLVGTIERLGGYTCKFGDDGVVTVSTVDRNKLRLVFKAHIIEMDGKILVDCRLSKGCGLEFKRRFIKIKNALEDIVLKGPTTWPIAIATNSVP

Target ORF information:

RefSeq Version	NM_057663.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grapes (grp), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057663.4

ORF Insert Sequence:

ATGGCTGCAA CGCTGACGGA AGCGGGAACA GGTCCTGCGG CCACCAGGGA GTTCGTCGAG 
GGATGGACTT TGGCCCAAAC TCTGGGCGAA GGTGCCTACG GCGAGGTAAA GCTACTAATC 
AACCGGCAGA CTGGCGAGGC TGTGGCCATG AAAATGGTGG ATCTAAAAAA ACATCCGGAT 
GCAGCGAACT CGGTGCGAAA GGAGGTATGC ATACAGAAGA TGCTCCAGGA TAAGCATATC 
CTCCGATTTT TCGGCAAACG TTCGCAAGGC AGTGTGGAGT ACATATTCCT GGAATACGCC 
GCCGGCGGAG AGCTATTCGA TCGAATAGAA CCAGACGTGG GAATGCCGCA GCATGAGGCT 
CAAAGGTATT TTACACAGCT CCTGTCCGGA CTCAATTACC TGCATCAGCG TGGGATCGCT 
CATCGGGATC TGAAGCCGGA AAATCTGCTG CTTGACGAGC ATGACAACGT GAAAATATCG 
GACTTTGGCA TGGCTACTAT GTTTAGGTGC AAGGGCAAGG AGCGACTGCT GGACAAACGC 
TGCGGCACCT TGCCGTATGT GGCGCCCGAG GTGCTACAGA AGGCATATCA CGCCCAGCCG 
GCGGATCTCT GGTCGTGTGG CGTTATATTG GTGACAATGC TGGCGGGTGA GCTGCCCTGG 
GATCAGCCGT CCACCAATTG CACGGAGTTC ACCAACTGGA GGGATAACGA TCACTGGCAA 
CTGCAGACTC CTTGGAGCAA ACTGGACACC TTGGCTATTT CGCTGCTTCG CAAGCTGCTG 
GCCACCAGTC CTGGCACGCG TTTGACCCTG GAGAAAACCC TGGATCACAA ATGGTGCAAC 
ATGCAGTTTG CAGACAATGA ACGTTCCTAT GACCTGGTGG ACTCGGCGGC TGCCCTGGAG 
ATATGCTCGC CAAAGGCTAA GAGGCAGCGT CTGCAGTCTA GTGCCCACTT GAGCAATGGC 
CTGGATGACT CCATCTCCCG GAACTACTGC TCTCAACCCA TGCCCACAAT GCGCAGCGAC 
GATGACTTCA ATGTCAGACT GGGCAGTGGC CGATCCAAGG AGGATGGAGG CGACCGCCAG 
ACGTTGGCCC AGGAGGCTCG GCTCAGTTAC TCCTTCTCGC AACCAGCTTT GCTTGATGAT 
CTCCTACTGG CTACCCAGAT GAACCAAACG CAGAACGCTT CCCAGAACTA TTTCCAGCGT 
TTGGTGAGGA GAATGACCCG ATTCTTTGTG ACCACACGAT GGGATGACAC TATCAAGCGA 
TTGGTGGGAA CCATCGAAAG ACTGGGTGGT TATACGTGCA AATTTGGTGA CGACGGAGTG 
GTCACCGTAT CCACAGTCGA TCGGAATAAG CTGCGATTGG TTTTCAAGGC ACACATTATA 
GAGATGGATG GCAAGATTCT TGTTGACTGC CGGCTGTCAA AGGGTTGCGG CTTGGAGTTT 
AAACGACGAT TTATCAAGAT CAAAAACGCC CTGGAGGATA TCGTGCTTAA AGGACCCACC 
ACCTGGCCGA TTGCGATTGC TACAAATTCG GTGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grp ( NM_165173.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723987.2 Drosophila melanogaster grapes (grp), transcript variant D, mRNA.

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CloneID	OFa14761
Clone ID Related Accession (Same CDS sequence)	NM_165173.3 , NM_001273579.1
Accession Version	NM_165173.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grapes, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165173.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCAGC ATGAGGCTCA AAGGTATTTT ACACAGCTCC TGTCCGGACT CAATTACCTG 
CATCAGCGTG GGATCGCTCA TCGGGATCTG AAGCCGGAAA ATCTGCTGCT TGACGAGCAT 
GACAACGTGA AAATATCGGA CTTTGGCATG GCTACTATGT TTAGGTGCAA GGGCAAGGAG 
CGACTGCTGG ACAAACGCTG CGGCACCTTG CCGTATGTGG CGCCCGAGGT GCTACAGAAG 
GCATATCACG CCCAGCCGGC GGATCTCTGG TCGTGTGGCG TTATATTGGT GACAATGCTG 
GCGGGTGAGC TGCCCTGGGA TCAGCCGTCC ACCAATTGCA CGGAGTTCAC CAACTGGAGG 
GATAACGATC ACTGGCAACT GCAGACTCCT TGGAGCAAAC TGGACACCTT GGCTATTTCG 
CTGCTTCGCA AGCTGCTGGC CACCAGTCCT GGCACGCGTT TGACCCTGGA GAAAACCCTG 
GATCACAAAT GGTGCAACAT GCAGTTTGCA GACAATGAAC GTTCCTATGA CCTGGTGGAC 
TCGGCGGCTG CCCTGGAGAT ATGCTCGCCA AAGGCTAAGA GGCAGCGTCT GCAGTCTAGT 
GCCCACTTGA GCAATGGCCT GGATGACTCC ATCTCCCGGA ACTACTGCTC TCAACCCATG 
CCCACAATGC GCAGCGACGA TGACTTCAAT GTCAGACTGG GCAGTGGCCG ATCCAAGGAG 
GATGGAGGCG ACCGCCAGAC GTTGGCCCAG GAGGCTCGGC TCAGTTACTC CTTCTCGCAA 
CCAGCTTTGC TTGATGATCT CCTACTGGCT ACCCAGATGA ACCAAACGCA GAACGCTTCC 
CAGAACTATT TCCAGCGTTT GGTGAGGAGA ATGACCCGAT TCTTTGTGAC CACACGATGG 
GATGACACTA TCAAGCGATT GGTGGGAACC ATCGAAAGAC TGGGTGGTTA TACGTGCAAA 
TTTGGTGACG ACGGAGTGGT CACCGTATCC ACAGTCGATC GGAATAAGCT GCGATTGGTT 
TTCAAGGCAC ACATTATAGA GATGGATGGC AAGATTCTTG TTGACTGCCG GCTGTCAAAG 
GGTTGCGGCT TGGAGTTTAA ACGACGATTT ATCAAGATCA AAAACGCCCT GGAGGATATC 
GTGCTTAAAG GACCCACCAC CTGGCCGATT GCGATTGCTA CAAATTCGGT GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723987.2
CDS	168..1364
Translation	MPQHEAQRYFTQLLSGLNYLHQRGIAHRDLKPENLLLDEHDNVKISDFGMATMFRCKGKERLLDKRCGTLPYVAPEVLQKAYHAQPADLWSCGVILVTMLAGELPWDQPSTNCTEFTNWRDNDHWQLQTPWSKLDTLAISLLRKLLATSPGTRLTLEKTLDHKWCNMQFADNERSYDLVDSAAALEICSPKAKRQRLQSSAHLSNGLDDSISRNYCSQPMPTMRSDDDFNVRLGSGRSKEDGGDRQTLAQEARLSYSFSQPALLDDLLLATQMNQTQNASQNYFQRLVRRMTRFFVTTRWDDTIKRLVGTIERLGGYTCKFGDDGVVTVSTVDRNKLRLVFKAHIIEMDGKILVDCRLSKGCGLEFKRRFIKIKNALEDIVLKGPTTWPIAIATNSVP

Target ORF information:

RefSeq Version	NM_165173.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grapes (grp), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165173.3

ORF Insert Sequence:

ATGCCGCAGC ATGAGGCTCA AAGGTATTTT ACACAGCTCC TGTCCGGACT CAATTACCTG 
CATCAGCGTG GGATCGCTCA TCGGGATCTG AAGCCGGAAA ATCTGCTGCT TGACGAGCAT 
GACAACGTGA AAATATCGGA CTTTGGCATG GCTACTATGT TTAGGTGCAA GGGCAAGGAG 
CGACTGCTGG ACAAACGCTG CGGCACCTTG CCGTATGTGG CGCCCGAGGT GCTACAGAAG 
GCATATCACG CCCAGCCGGC GGATCTCTGG TCGTGTGGCG TTATATTGGT GACAATGCTG 
GCGGGTGAGC TGCCCTGGGA TCAGCCGTCC ACCAATTGCA CGGAGTTCAC CAACTGGAGG 
GATAACGATC ACTGGCAACT GCAGACTCCT TGGAGCAAAC TGGACACCTT GGCTATTTCG 
CTGCTTCGCA AGCTGCTGGC CACCAGTCCT GGCACGCGTT TGACCCTGGA GAAAACCCTG 
GATCACAAAT GGTGCAACAT GCAGTTTGCA GACAATGAAC GTTCCTATGA CCTGGTGGAC 
TCGGCGGCTG CCCTGGAGAT ATGCTCGCCA AAGGCTAAGA GGCAGCGTCT GCAGTCTAGT 
GCCCACTTGA GCAATGGCCT GGATGACTCC ATCTCCCGGA ACTACTGCTC TCAACCCATG 
CCCACAATGC GCAGCGACGA TGACTTCAAT GTCAGACTGG GCAGTGGCCG ATCCAAGGAG 
GATGGAGGCG ACCGCCAGAC GTTGGCCCAG GAGGCTCGGC TCAGTTACTC CTTCTCGCAA 
CCAGCTTTGC TTGATGATCT CCTACTGGCT ACCCAGATGA ACCAAACGCA GAACGCTTCC 
CAGAACTATT TCCAGCGTTT GGTGAGGAGA ATGACCCGAT TCTTTGTGAC CACACGATGG 
GATGACACTA TCAAGCGATT GGTGGGAACC ATCGAAAGAC TGGGTGGTTA TACGTGCAAA 
TTTGGTGACG ACGGAGTGGT CACCGTATCC ACAGTCGATC GGAATAAGCT GCGATTGGTT 
TTCAAGGCAC ACATTATAGA GATGGATGGC AAGATTCTTG TTGACTGCCG GCTGTCAAAG 
GGTTGCGGCT TGGAGTTTAA ACGACGATTT ATCAAGATCA AAAACGCCCT GGAGGATATC 
GTGCTTAAAG GACCCACCAC CTGGCCGATT GCGATTGCTA CAAATTCGGT GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grp ( NM_001273579.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260508.1 Drosophila melanogaster grapes (grp), transcript variant F, mRNA.

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CloneID	OFa14761
Clone ID Related Accession (Same CDS sequence)	NM_165173.3 , NM_001273579.1
Accession Version	NM_001273579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grapes, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCAGC ATGAGGCTCA AAGGTATTTT ACACAGCTCC TGTCCGGACT CAATTACCTG 
CATCAGCGTG GGATCGCTCA TCGGGATCTG AAGCCGGAAA ATCTGCTGCT TGACGAGCAT 
GACAACGTGA AAATATCGGA CTTTGGCATG GCTACTATGT TTAGGTGCAA GGGCAAGGAG 
CGACTGCTGG ACAAACGCTG CGGCACCTTG CCGTATGTGG CGCCCGAGGT GCTACAGAAG 
GCATATCACG CCCAGCCGGC GGATCTCTGG TCGTGTGGCG TTATATTGGT GACAATGCTG 
GCGGGTGAGC TGCCCTGGGA TCAGCCGTCC ACCAATTGCA CGGAGTTCAC CAACTGGAGG 
GATAACGATC ACTGGCAACT GCAGACTCCT TGGAGCAAAC TGGACACCTT GGCTATTTCG 
CTGCTTCGCA AGCTGCTGGC CACCAGTCCT GGCACGCGTT TGACCCTGGA GAAAACCCTG 
GATCACAAAT GGTGCAACAT GCAGTTTGCA GACAATGAAC GTTCCTATGA CCTGGTGGAC 
TCGGCGGCTG CCCTGGAGAT ATGCTCGCCA AAGGCTAAGA GGCAGCGTCT GCAGTCTAGT 
GCCCACTTGA GCAATGGCCT GGATGACTCC ATCTCCCGGA ACTACTGCTC TCAACCCATG 
CCCACAATGC GCAGCGACGA TGACTTCAAT GTCAGACTGG GCAGTGGCCG ATCCAAGGAG 
GATGGAGGCG ACCGCCAGAC GTTGGCCCAG GAGGCTCGGC TCAGTTACTC CTTCTCGCAA 
CCAGCTTTGC TTGATGATCT CCTACTGGCT ACCCAGATGA ACCAAACGCA GAACGCTTCC 
CAGAACTATT TCCAGCGTTT GGTGAGGAGA ATGACCCGAT TCTTTGTGAC CACACGATGG 
GATGACACTA TCAAGCGATT GGTGGGAACC ATCGAAAGAC TGGGTGGTTA TACGTGCAAA 
TTTGGTGACG ACGGAGTGGT CACCGTATCC ACAGTCGATC GGAATAAGCT GCGATTGGTT 
TTCAAGGCAC ACATTATAGA GATGGATGGC AAGATTCTTG TTGACTGCCG GCTGTCAAAG 
GGTTGCGGCT TGGAGTTTAA ACGACGATTT ATCAAGATCA AAAACGCCCT GGAGGATATC 
GTGCTTAAAG GACCCACCAC CTGGCCGATT GCGATTGCTA CAAATTCGGT GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260508.1
CDS	529..1725
Translation	MPQHEAQRYFTQLLSGLNYLHQRGIAHRDLKPENLLLDEHDNVKISDFGMATMFRCKGKERLLDKRCGTLPYVAPEVLQKAYHAQPADLWSCGVILVTMLAGELPWDQPSTNCTEFTNWRDNDHWQLQTPWSKLDTLAISLLRKLLATSPGTRLTLEKTLDHKWCNMQFADNERSYDLVDSAAALEICSPKAKRQRLQSSAHLSNGLDDSISRNYCSQPMPTMRSDDDFNVRLGSGRSKEDGGDRQTLAQEARLSYSFSQPALLDDLLLATQMNQTQNASQNYFQRLVRRMTRFFVTTRWDDTIKRLVGTIERLGGYTCKFGDDGVVTVSTVDRNKLRLVFKAHIIEMDGKILVDCRLSKGCGLEFKRRFIKIKNALEDIVLKGPTTWPIAIATNSVP

Target ORF information:

RefSeq Version	NM_001273579.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grapes (grp), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273579.1

ORF Insert Sequence:

ATGCCGCAGC ATGAGGCTCA AAGGTATTTT ACACAGCTCC TGTCCGGACT CAATTACCTG 
CATCAGCGTG GGATCGCTCA TCGGGATCTG AAGCCGGAAA ATCTGCTGCT TGACGAGCAT 
GACAACGTGA AAATATCGGA CTTTGGCATG GCTACTATGT TTAGGTGCAA GGGCAAGGAG 
CGACTGCTGG ACAAACGCTG CGGCACCTTG CCGTATGTGG CGCCCGAGGT GCTACAGAAG 
GCATATCACG CCCAGCCGGC GGATCTCTGG TCGTGTGGCG TTATATTGGT GACAATGCTG 
GCGGGTGAGC TGCCCTGGGA TCAGCCGTCC ACCAATTGCA CGGAGTTCAC CAACTGGAGG 
GATAACGATC ACTGGCAACT GCAGACTCCT TGGAGCAAAC TGGACACCTT GGCTATTTCG 
CTGCTTCGCA AGCTGCTGGC CACCAGTCCT GGCACGCGTT TGACCCTGGA GAAAACCCTG 
GATCACAAAT GGTGCAACAT GCAGTTTGCA GACAATGAAC GTTCCTATGA CCTGGTGGAC 
TCGGCGGCTG CCCTGGAGAT ATGCTCGCCA AAGGCTAAGA GGCAGCGTCT GCAGTCTAGT 
GCCCACTTGA GCAATGGCCT GGATGACTCC ATCTCCCGGA ACTACTGCTC TCAACCCATG 
CCCACAATGC GCAGCGACGA TGACTTCAAT GTCAGACTGG GCAGTGGCCG ATCCAAGGAG 
GATGGAGGCG ACCGCCAGAC GTTGGCCCAG GAGGCTCGGC TCAGTTACTC CTTCTCGCAA 
CCAGCTTTGC TTGATGATCT CCTACTGGCT ACCCAGATGA ACCAAACGCA GAACGCTTCC 
CAGAACTATT TCCAGCGTTT GGTGAGGAGA ATGACCCGAT TCTTTGTGAC CACACGATGG 
GATGACACTA TCAAGCGATT GGTGGGAACC ATCGAAAGAC TGGGTGGTTA TACGTGCAAA 
TTTGGTGACG ACGGAGTGGT CACCGTATCC ACAGTCGATC GGAATAAGCT GCGATTGGTT 
TTCAAGGCAC ACATTATAGA GATGGATGGC AAGATTCTTG TTGACTGCCG GCTGTCAAAG 
GGTTGCGGCT TGGAGTTTAA ACGACGATTT ATCAAGATCA AAAACGCCCT GGAGGATATC 
GTGCTTAAAG GACCCACCAC CTGGCCGATT GCGATTGCTA CAAATTCGGT GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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grp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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