Nepl8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepl8
Entrez Gene ID	34983
Full Name	CG4580 gene product from transcript CG4580-RA
Synonyms	Dmel\CG4580
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 36A2-36A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_135951.4	NP_609795.2	Neprilysin-like 8

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG4580	NP_609795.2
Drosophila melanogaster (fruit fly)	CG9505	NP_609019.1
Drosophila melanogaster (fruit fly)	CG9507	NP_609022.1
Drosophila melanogaster (fruit fly)	CG31918	NP_608904.1
Drosophila melanogaster (fruit fly)	CG8550	NP_610787.1
Drosophila melanogaster (fruit fly)	CG42370	NP_609023.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepl8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepl8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14750	NM_135951.4 Latest version!	Drosophila melanogaster Neprilysin-like 8 (Nepl8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepl8 ( NM_135951.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609795.2 Drosophila melanogaster Neprilysin-like 8 (Nepl8), mRNA.

Price & Availability↑

CloneID	OFa14750
Clone ID Related Accession (Same CDS sequence)	NM_135951.4
Accession Version	NM_135951.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neprilysin-like 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135951.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGATTA TCTGCCTCTT GCTGGTGCCC CTTGTGGCGG CCAGTTCCAA TACAAGACTT 
CTAAATGGCA TCCTAAGTCA TGTGGACAAG GAAGCCAATC CCTGTGAGAA CTACTACAAC 
CACGCCTGCG GCCAGTACAA CATGCGTCAC ATCGACGACA CCTTCTTCGA CATTATACAA 
ATGCTGGATC ACCAGGTTAA CCAGAACTTG GTGAAACTAA TGGACGAGCT GGAAATGAGT 
TCTCAATTGC CGGACTTTAA TGTATCTAGT GTAGATGGCA AGGTCCTTCG TTACTACCTT 
AGTTGTCGTG GAGCGCCGCG GAATATGGAT AGTTTAAGCC AGTATCTGAA AGTGATTTCC 
CCCGGCGAAG GACTCACATG GCCTCAATTC ATTCCGGACG GTAGTTCTTG GCCCCAGGAG 
AATTTCAAAT GGCTCAAGGC ACTGGCTCAT CTGCATCGCT ACGGTCTAAC TAACGTGTTT 
TTTAACCTTG AAGTCGTGTC AAACCCACGA AATGCCAGCG AGTACATGGT AGAATTAAAT 
ACACCCACTT TTGGAGAAGA ATCTCAACTG CCGAACAGTT TTATTGAAAT TCTATCCGTT 
CTCTATATCA TAAAGGTTCC TTCCAGTGAA ATCATTACTC TGGCGCGAAA AATGCGAACG 
CTTGAATTGT TGCTTAAAAC GATGATCAAT CCGATCGACA CACTGAATAA TAGATACATT 
AGTATCCGCG ATTTTCAGAT GGAAACCGGT CACAACTGGC AGCGTTTCTT TGAGATTTTA 
ATAGGCTCCA GCGCAGCCCC AGAACTCCAA GTGTTGGTGC GCAATTTTAG GTACTTTACC 
GCCCTTAAGG AACTAATGGA CAAACAGGAT GCTCGGCTGG TGGCCAGCTA CATAATGACC 
CGATTTGCAA TATTTCTATT GGATGAAACC ATGGGTGGCA GAGAATCCAC GGAGTGTGTG 
TCACAGGTGC GCCGCAACAT GAATTTGGCT GCAAACATGC TCTATAAGGA ACGATTTTTC 
GAAGACTCCA CTTTCAGTGC CAATATCCTG GAAATTAAGG ACATTTTCGA GAAACTACGC 
CATCAGTTTC TGCTGCAAGT CGATCAAAAT CATCTAGAGT TGACTGCTTT GCAGATGAAA 
TTTTTCGTTC GAAAGGCAGA GGCAATTGAG ATCAACGTTG TGAATCTTCC AAAAACCGAT 
GATCTTCGCC ATTTCATCGG CCAGTACTAC CAAGACTTGC AGTTTCCCAC TGGCGAGCTG 
GATTACCATC AGGAGCACCT CAAGGTGCTG CAGTTTCGCA CCCAAAAGAT GTTGGCCCAA 
TCCAGCAAAG GGCACTCAGA GGAGCAGAAT ATTTTGACTT ACAGGAGCCA AGCGGCGCCA 
TTGCCTCCAC CTCAGCCATT CTTTCAGCTG GAAAGCGAAG ATGTCTTCAA ATACAGCCTG 
ATGGGATATA TTATGGCACA TCACTTGATA AGCGCCTTTG CCACCGAGGG CATTACAATT 
GGCAGCGATG GAAACGATCA ATCATTTAGA TCGCATCGTT TCGAAGAAGC AGTCAGTTGC 
TTGTCACGCA ATTCAGAGAA CATCGATGAA AGCATGGGCG ATATTGCTGG TTTAGAACTG 
GCCTATTTTA CTTATGCTAA GATGGCCAAG AATCGAAACC GTTTGGATTT CACCCATTTG 
CCACCGGAGC AGATATTCTT CCTAAATGTT GGCCAGTTCT TCTGCGGCAA TAGCGATATG 
TTGGTTCAGT ACAAGGAAGA TCAAGTGCGT TTACAGCGAG CTATTGAAGG GTTTGAGCCA 
TTTGACAAGG CTTTTGGGTG CTACCGCAAT AAGCCTAAGC ACGAGAAGTG TCGTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609795.2
CDS	56..1915
Translation	MWIICLLLVPLVAASSNTRLLNGILSHVDKEANPCENYYNHACGQYNMRHIDDTFFDIIQMLDHQVNQNLVKLMDELEMSSQLPDFNVSSVDGKVLRYYLSCRGAPRNMDSLSQYLKVISPGEGLTWPQFIPDGSSWPQENFKWLKALAHLHRYGLTNVFFNLEVVSNPRNASEYMVELNTPTFGEESQLPNSFIEILSVLYIIKVPSSEIITLARKMRTLELLLKTMINPIDTLNNRYISIRDFQMETGHNWQRFFEILIGSSAAPELQVLVRNFRYFTALKELMDKQDARLVASYIMTRFAIFLLDETMGGRESTECVSQVRRNMNLAANMLYKERFFEDSTFSANILEIKDIFEKLRHQFLLQVDQNHLELTALQMKFFVRKAEAIEINVVNLPKTDDLRHFIGQYYQDLQFPTGELDYHQEHLKVLQFRTQKMLAQSSKGHSEEQNILTYRSQAAPLPPPQPFFQLESEDVFKYSLMGYIMAHHLISAFATEGITIGSDGNDQSFRSHRFEEAVSCLSRNSENIDESMGDIAGLELAYFTYAKMAKNRNRLDFTHLPPEQIFFLNVGQFFCGNSDMLVQYKEDQVRLQRAIEGFEPFDKAFGCYRNKPKHEKCRL

Target ORF information:

RefSeq Version	NM_135951.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neprilysin-like 8 (Nepl8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135951.4

ORF Insert Sequence:

ATGTGGATTA TCTGCCTCTT GCTGGTGCCC CTTGTGGCGG CCAGTTCCAA TACAAGACTT 
CTAAATGGCA TCCTAAGTCA TGTGGACAAG GAAGCCAATC CCTGTGAGAA CTACTACAAC 
CACGCCTGCG GCCAGTACAA CATGCGTCAC ATCGACGACA CCTTCTTCGA CATTATACAA 
ATGCTGGATC ACCAGGTTAA CCAGAACTTG GTGAAACTAA TGGACGAGCT GGAAATGAGT 
TCTCAATTGC CGGACTTTAA TGTATCTAGT GTAGATGGCA AGGTCCTTCG TTACTACCTT 
AGTTGTCGTG GAGCGCCGCG GAATATGGAT AGTTTAAGCC AGTATCTGAA AGTGATTTCC 
CCCGGCGAAG GACTCACATG GCCTCAATTC ATTCCGGACG GTAGTTCTTG GCCCCAGGAG 
AATTTCAAAT GGCTCAAGGC ACTGGCTCAT CTGCATCGCT ACGGTCTAAC TAACGTGTTT 
TTTAACCTTG AAGTCGTGTC AAACCCACGA AATGCCAGCG AGTACATGGT AGAATTAAAT 
ACACCCACTT TTGGAGAAGA ATCTCAACTG CCGAACAGTT TTATTGAAAT TCTATCCGTT 
CTCTATATCA TAAAGGTTCC TTCCAGTGAA ATCATTACTC TGGCGCGAAA AATGCGAACG 
CTTGAATTGT TGCTTAAAAC GATGATCAAT CCGATCGACA CACTGAATAA TAGATACATT 
AGTATCCGCG ATTTTCAGAT GGAAACCGGT CACAACTGGC AGCGTTTCTT TGAGATTTTA 
ATAGGCTCCA GCGCAGCCCC AGAACTCCAA GTGTTGGTGC GCAATTTTAG GTACTTTACC 
GCCCTTAAGG AACTAATGGA CAAACAGGAT GCTCGGCTGG TGGCCAGCTA CATAATGACC 
CGATTTGCAA TATTTCTATT GGATGAAACC ATGGGTGGCA GAGAATCCAC GGAGTGTGTG 
TCACAGGTGC GCCGCAACAT GAATTTGGCT GCAAACATGC TCTATAAGGA ACGATTTTTC 
GAAGACTCCA CTTTCAGTGC CAATATCCTG GAAATTAAGG ACATTTTCGA GAAACTACGC 
CATCAGTTTC TGCTGCAAGT CGATCAAAAT CATCTAGAGT TGACTGCTTT GCAGATGAAA 
TTTTTCGTTC GAAAGGCAGA GGCAATTGAG ATCAACGTTG TGAATCTTCC AAAAACCGAT 
GATCTTCGCC ATTTCATCGG CCAGTACTAC CAAGACTTGC AGTTTCCCAC TGGCGAGCTG 
GATTACCATC AGGAGCACCT CAAGGTGCTG CAGTTTCGCA CCCAAAAGAT GTTGGCCCAA 
TCCAGCAAAG GGCACTCAGA GGAGCAGAAT ATTTTGACTT ACAGGAGCCA AGCGGCGCCA 
TTGCCTCCAC CTCAGCCATT CTTTCAGCTG GAAAGCGAAG ATGTCTTCAA ATACAGCCTG 
ATGGGATATA TTATGGCACA TCACTTGATA AGCGCCTTTG CCACCGAGGG CATTACAATT 
GGCAGCGATG GAAACGATCA ATCATTTAGA TCGCATCGTT TCGAAGAAGC AGTCAGTTGC 
TTGTCACGCA ATTCAGAGAA CATCGATGAA AGCATGGGCG ATATTGCTGG TTTAGAACTG 
GCCTATTTTA CTTATGCTAA GATGGCCAAG AATCGAAACC GTTTGGATTT CACCCATTTG 
CCACCGGAGC AGATATTCTT CCTAAATGTT GGCCAGTTCT TCTGCGGCAA TAGCGATATG 
TTGGTTCAGT ACAAGGAAGA TCAAGTGCGT TTACAGCGAG CTATTGAAGG GTTTGAGCCA 
TTTGACAAGG CTTTTGGGTG CTACCGCAAT AAGCCTAAGC ACGAGAAGTG TCGTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nepl8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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