cact cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	cact
Entrez Gene ID	34969
Full Name	cactus
Synonyms	5848,BG:DS02740.15,CACT,CG5848,Cact,Cactus,Dmel\CG5848,Ikappa,NFkappabeta1A,cac,dip6,fs(2)ltoRN48,n(2)k17003
General protein information	Preferred Names cactus Names CG5848 gene product from transcript CG5848-RB CG5848-PA CG5848-PB CG5848-PC CG5848-PD CG5848-PE NF-kappa-B inhibitor cactus cact-PA cact-PB cact-PC cact-PD cact-PE female-sterile-(2)-ltoRN48
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35F1-35F1\|2-52 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057595.4	NP_476943.1	cactus, isoform C
NM_205999.2	NP_995721.1	cactus, isoform D
NM_001273567.1	NP_001260496.1	cactus, isoform E
NM_165152.2	NP_723960.1	cactus, isoform A
NM_057594.4	NP_476942.1	cactus, isoform B

Pathways from BioSystems

Homologies

	NFKBIA	XP_001087842.1
Canis lupus familiaris (dog)	NFKBIA	XP_537413.3
Bos taurus (cattle)	NFKBIA	NP_001039333.1
Danio rerio (zebrafish)	nfkbiaa	NP_998349.1
Pan troglodytes (chimpanzee)	NFKBIA	XP_522823.2
Rattus norvegicus (Norway rat)	Nfkbia	NP_001099190.2
Gallus gallus (chicken)	NFKBIA	NP_001001472.2
Danio rerio (zebrafish)	nfkbiab	NP_955923.1
Anopheles gambiae (African malaria mosquito)	CACT	XP_317542.4
Homo sapiens (human)	NFKBIA	NP_065390.1
Mus musculus (house mouse)	Nfkbia	NP_035037.2
Xenopus tropicalis (tropical clawed frog)	nfkbia	NP_001004851.1
Drosophila melanogaster (fruit fly)	cact	NP_001260496.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It has been shown that Dorsal repressed Ush expression levels to promote hemocytes differentiation, whereas Cactus maintained Ush levels to block differentiation.
Title: The Friend of GATA Transcriptional Co-Regulator, U-Shaped, Is a Downstream Antagonist of Dorsal-Driven Prohemocyte Differentiation in Drosophila.

CalpA targets free Cactus, which is incorporated into and modulates Toll-responsive complexes in the embryo and immune system.
Title: Calpain A modulates Toll responses by limited Cactus/I?B proteolysis.

Dpp signals increase Cactus levels through Calpain A inhibition, thereby interfering with Dorsal activation
Title: The Ca2+-dependent protease Calpain A regulates Cactus/I kappaB levels during Drosophila development in response to maternal Dpp signals.

immune-induced degradation does not require CSN5
Title: COP9 signalosome subunit 5 (CSN5/Jab1) regulates the development of the Drosophila immune system: effects on Cactus, Dorsal and hematopoiesis.

Cactus acts as an inhibitor of the Rel-transcription factors Dorsal and Dif.
Title: The inhibitor kappaB-ortholog Cactus is necessary for normal neuromuscular function in Drosophila melanogaster.

The following cact gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cact cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14736	NM_057594.4 Latest version!	Drosophila melanogaster cactus (cact), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14736	NM_205999.2 Latest version!	Drosophila melanogaster cactus (cact), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14736	NM_001273567.1 Latest version!	Drosophila melanogaster cactus (cact), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14736	NM_165152.2 Latest version!	Drosophila melanogaster cactus (cact), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16831	NM_057595.4 Latest version!	Drosophila melanogaster cactus (cact), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cact ( NM_205999.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995721.1 Drosophila melanogaster cactus (cact), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa14736
Clone ID Related Accession (Same CDS sequence)	NM_205999.2 , NM_001273567.1 , NM_165152.2 , NM_057594.4
Accession Version	NM_205999.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cactus, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_205999.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAGCC CAACAAAAGC AGCGGAGGCA GCAACAAAGG CAACAGCAAC AAGTGACTGC 
AGCTGCAGCG CTGCCAGTGT TGAGCAGCGC GCCCCCTCAA ACGCGGCTAA CCCCTCGTCC 
TCACTAGCCA CTAGCGGTAA AATTGGCGGC AAAACACAAG ATCAAACGGC CGCCATTAAC 
AAGCAAAAGG AATTTGCCGT GCCAAACGAA ACCAGTGATT CGGGCTTCAT CTCCGGCCCG 
CAATCCTCGC AGATCTTCAG CGAGGAGATA GTCCCCGATA GCGAGGAACA GGATAAGGAT 
CAGCAGGAGT CGGCACCCCA AAAGGAACAG CCCGTTGTCC TCGATTCCGG TATTATCGAC 
GAAGAAGAGG ATCAGGAGGA GCAGGAGAAA GAGGAGGAGC ACCAGGACAC CACCACTGCC 
ACCGCCGATT CAATGCGACT CAAACACAGC GCGGACACAG GCATCCCACA ATGGACCGTC 
GAAAGCCATT TGGTCAGTCG CGGCGAACAG CTTAACAATC TCGGCCAGAG CAGCAGCACC 
CAAATCACCG GTCGCAGCAA GGTTCAATCC TCCACAGCCA GCACGGCGAA CGCGAATCCT 
TCGGGATCGG GAGCAACATC ATCCGCTCCG CCCAGCAGCA TTAATATCAT GAACGCCTGG 
GAGCAGTTCT ATCAACAAAA CGACGACGGC GACACACCTT TGCACCTGGC CTGTATTTCG 
GGATCAGTGG ACGTGGTGGC CGCCCTGATT CGCATGGCTC CTCATCCGTG CCTGCTCAAC 
ATCCAGAACG ATGTGGCCCA GACACCCCTT CATCTGGCCG CTCTTACTGC CCAGCCGAAC 
ATCATGAGGA TACTTTTACT CGCCGGAGCT GAGCCCACGG TGCGCGATCG TCATGGAAAC 
ACAGCTCTGC ATCTTTCCTG CATCGCCGGA GAGAAACAGT GCGTCCGTGC CTTGACAGAG 
AAGTTCGGCG CCACGGAAAT CCATGAGGCC CACCGACAAT ATGGACACCG ATCCAACGAC 
AAAGCGGTCA GTTCCCTGAG CTACGCCTGC CTGCCCGCCG ATCTGGAGAT TCGCAACTAC 
GATGGAGAAC GCTGTGTGCA TTTGGCCGCC GAGGCGGGTC ACATTGATAT CTTACGCATT 
TTGGTATCCC ATGGAGCAGA CATTAACGCC AGGGAGGGAA AATCGGGCCG CACACCGCTG 
CACATTGCGA TCGAGGGCTG CAACGAGGAT CTGGCCAACT TCCTGCTCGA TGAGTGCGAG 
AAGCTGAACC TGGAAACGGC CACCTACGCC GGCTTGACGG CCTACCAGTT TGCGTGCATC 
ATGAACAAGT CGCGCATGCA GAACATCCTG GAGAAGCGCG GAGCGGAGAC CGTAACGCCT 
CCGGACAGTG ATTACGACAG TAGCGATATC GAAGATCTAG ACGATACGAA GATGTACGAT 
CGCTTTGGTG ATCCTCGCTA TTTTGTCAGC TACAACGGTG GCAATCCCAT GACAGTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995721.1
CDS	394..1896
Translation	MPSPTKAAEAATKATATSDCSCSAASVEQRAPSNAANPSSSLATSGKIGGKTQDQTAAINKQKEFAVPNETSDSGFISGPQSSQIFSEEIVPDSEEQDKDQQESAPQKEQPVVLDSGIIDEEEDQEEQEKEEEHQDTTTATADSMRLKHSADTGIPQWTVESHLVSRGEQLNNLGQSSSTQITGRSKVQSSTASTANANPSGSGATSSAPPSSINIMNAWEQFYQQNDDGDTPLHLACISGSVDVVAALIRMAPHPCLLNIQNDVAQTPLHLAALTAQPNIMRILLLAGAEPTVRDRHGNTALHLSCIAGEKQCVRALTEKFGATEIHEAHRQYGHRSNDKAVSSLSYACLPADLEIRNYDGERCVHLAAEAGHIDILRILVSHGADINAREGKSGRTPLHIAIEGCNEDLANFLLDECEKLNLETATYAGLTAYQFACIMNKSRMQNILEKRGAETVTPPDSDYDSSDIEDLDDTKMYDRFGDPRYFVSYNGGNPMTVA

Target ORF information:

RefSeq Version	NM_205999.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cactus (cact), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205999.2

ORF Insert Sequence:

ATGCCGAGCC CAACAAAAGC AGCGGAGGCA GCAACAAAGG CAACAGCAAC AAGTGACTGC 
AGCTGCAGCG CTGCCAGTGT TGAGCAGCGC GCCCCCTCAA ACGCGGCTAA CCCCTCGTCC 
TCACTAGCCA CTAGCGGTAA AATTGGCGGC AAAACACAAG ATCAAACGGC CGCCATTAAC 
AAGCAAAAGG AATTTGCCGT GCCAAACGAA ACCAGTGATT CGGGCTTCAT CTCCGGCCCG 
CAATCCTCGC AGATCTTCAG CGAGGAGATA GTCCCCGATA GCGAGGAACA GGATAAGGAT 
CAGCAGGAGT CGGCACCCCA AAAGGAACAG CCCGTTGTCC TCGATTCCGG TATTATCGAC 
GAAGAAGAGG ATCAGGAGGA GCAGGAGAAA GAGGAGGAGC ACCAGGACAC CACCACTGCC 
ACCGCCGATT CAATGCGACT CAAACACAGC GCGGACACAG GCATCCCACA ATGGACCGTC 
GAAAGCCATT TGGTCAGTCG CGGCGAACAG CTTAACAATC TCGGCCAGAG CAGCAGCACC 
CAAATCACCG GTCGCAGCAA GGTTCAATCC TCCACAGCCA GCACGGCGAA CGCGAATCCT 
TCGGGATCGG GAGCAACATC ATCCGCTCCG CCCAGCAGCA TTAATATCAT GAACGCCTGG 
GAGCAGTTCT ATCAACAAAA CGACGACGGC GACACACCTT TGCACCTGGC CTGTATTTCG 
GGATCAGTGG ACGTGGTGGC CGCCCTGATT CGCATGGCTC CTCATCCGTG CCTGCTCAAC 
ATCCAGAACG ATGTGGCCCA GACACCCCTT CATCTGGCCG CTCTTACTGC CCAGCCGAAC 
ATCATGAGGA TACTTTTACT CGCCGGAGCT GAGCCCACGG TGCGCGATCG TCATGGAAAC 
ACAGCTCTGC ATCTTTCCTG CATCGCCGGA GAGAAACAGT GCGTCCGTGC CTTGACAGAG 
AAGTTCGGCG CCACGGAAAT CCATGAGGCC CACCGACAAT ATGGACACCG ATCCAACGAC 
AAAGCGGTCA GTTCCCTGAG CTACGCCTGC CTGCCCGCCG ATCTGGAGAT TCGCAACTAC 
GATGGAGAAC GCTGTGTGCA TTTGGCCGCC GAGGCGGGTC ACATTGATAT CTTACGCATT 
TTGGTATCCC ATGGAGCAGA CATTAACGCC AGGGAGGGAA AATCGGGCCG CACACCGCTG 
CACATTGCGA TCGAGGGCTG CAACGAGGAT CTGGCCAACT TCCTGCTCGA TGAGTGCGAG 
AAGCTGAACC TGGAAACGGC CACCTACGCC GGCTTGACGG CCTACCAGTT TGCGTGCATC 
ATGAACAAGT CGCGCATGCA GAACATCCTG GAGAAGCGCG GAGCGGAGAC CGTAACGCCT 
CCGGACAGTG ATTACGACAG TAGCGATATC GAAGATCTAG ACGATACGAA GATGTACGAT 
CGCTTTGGTG ATCCTCGCTA TTTTGTCAGC TACAACGGTG GCAATCCCAT GACAGTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cact ( NM_001273567.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260496.1 Drosophila melanogaster cactus (cact), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa14736
Clone ID Related Accession (Same CDS sequence)	NM_205999.2 , NM_001273567.1 , NM_165152.2 , NM_057594.4
Accession Version	NM_001273567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cactus, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAGCC CAACAAAAGC AGCGGAGGCA GCAACAAAGG CAACAGCAAC AAGTGACTGC 
AGCTGCAGCG CTGCCAGTGT TGAGCAGCGC GCCCCCTCAA ACGCGGCTAA CCCCTCGTCC 
TCACTAGCCA CTAGCGGTAA AATTGGCGGC AAAACACAAG ATCAAACGGC CGCCATTAAC 
AAGCAAAAGG AATTTGCCGT GCCAAACGAA ACCAGTGATT CGGGCTTCAT CTCCGGCCCG 
CAATCCTCGC AGATCTTCAG CGAGGAGATA GTCCCCGATA GCGAGGAACA GGATAAGGAT 
CAGCAGGAGT CGGCACCCCA AAAGGAACAG CCCGTTGTCC TCGATTCCGG TATTATCGAC 
GAAGAAGAGG ATCAGGAGGA GCAGGAGAAA GAGGAGGAGC ACCAGGACAC CACCACTGCC 
ACCGCCGATT CAATGCGACT CAAACACAGC GCGGACACAG GCATCCCACA ATGGACCGTC 
GAAAGCCATT TGGTCAGTCG CGGCGAACAG CTTAACAATC TCGGCCAGAG CAGCAGCACC 
CAAATCACCG GTCGCAGCAA GGTTCAATCC TCCACAGCCA GCACGGCGAA CGCGAATCCT 
TCGGGATCGG GAGCAACATC ATCCGCTCCG CCCAGCAGCA TTAATATCAT GAACGCCTGG 
GAGCAGTTCT ATCAACAAAA CGACGACGGC GACACACCTT TGCACCTGGC CTGTATTTCG 
GGATCAGTGG ACGTGGTGGC CGCCCTGATT CGCATGGCTC CTCATCCGTG CCTGCTCAAC 
ATCCAGAACG ATGTGGCCCA GACACCCCTT CATCTGGCCG CTCTTACTGC CCAGCCGAAC 
ATCATGAGGA TACTTTTACT CGCCGGAGCT GAGCCCACGG TGCGCGATCG TCATGGAAAC 
ACAGCTCTGC ATCTTTCCTG CATCGCCGGA GAGAAACAGT GCGTCCGTGC CTTGACAGAG 
AAGTTCGGCG CCACGGAAAT CCATGAGGCC CACCGACAAT ATGGACACCG ATCCAACGAC 
AAAGCGGTCA GTTCCCTGAG CTACGCCTGC CTGCCCGCCG ATCTGGAGAT TCGCAACTAC 
GATGGAGAAC GCTGTGTGCA TTTGGCCGCC GAGGCGGGTC ACATTGATAT CTTACGCATT 
TTGGTATCCC ATGGAGCAGA CATTAACGCC AGGGAGGGAA AATCGGGCCG CACACCGCTG 
CACATTGCGA TCGAGGGCTG CAACGAGGAT CTGGCCAACT TCCTGCTCGA TGAGTGCGAG 
AAGCTGAACC TGGAAACGGC CACCTACGCC GGCTTGACGG CCTACCAGTT TGCGTGCATC 
ATGAACAAGT CGCGCATGCA GAACATCCTG GAGAAGCGCG GAGCGGAGAC CGTAACGCCT 
CCGGACAGTG ATTACGACAG TAGCGATATC GAAGATCTAG ACGATACGAA GATGTACGAT 
CGCTTTGGTG ATCCTCGCTA TTTTGTCAGC TACAACGGTG GCAATCCCAT GACAGTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260496.1
CDS	215..1717
Translation	MPSPTKAAEAATKATATSDCSCSAASVEQRAPSNAANPSSSLATSGKIGGKTQDQTAAINKQKEFAVPNETSDSGFISGPQSSQIFSEEIVPDSEEQDKDQQESAPQKEQPVVLDSGIIDEEEDQEEQEKEEEHQDTTTATADSMRLKHSADTGIPQWTVESHLVSRGEQLNNLGQSSSTQITGRSKVQSSTASTANANPSGSGATSSAPPSSINIMNAWEQFYQQNDDGDTPLHLACISGSVDVVAALIRMAPHPCLLNIQNDVAQTPLHLAALTAQPNIMRILLLAGAEPTVRDRHGNTALHLSCIAGEKQCVRALTEKFGATEIHEAHRQYGHRSNDKAVSSLSYACLPADLEIRNYDGERCVHLAAEAGHIDILRILVSHGADINAREGKSGRTPLHIAIEGCNEDLANFLLDECEKLNLETATYAGLTAYQFACIMNKSRMQNILEKRGAETVTPPDSDYDSSDIEDLDDTKMYDRFGDPRYFVSYNGGNPMTVA

Target ORF information:

RefSeq Version	NM_001273567.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cactus (cact), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273567.1

ORF Insert Sequence:

ATGCCGAGCC CAACAAAAGC AGCGGAGGCA GCAACAAAGG CAACAGCAAC AAGTGACTGC 
AGCTGCAGCG CTGCCAGTGT TGAGCAGCGC GCCCCCTCAA ACGCGGCTAA CCCCTCGTCC 
TCACTAGCCA CTAGCGGTAA AATTGGCGGC AAAACACAAG ATCAAACGGC CGCCATTAAC 
AAGCAAAAGG AATTTGCCGT GCCAAACGAA ACCAGTGATT CGGGCTTCAT CTCCGGCCCG 
CAATCCTCGC AGATCTTCAG CGAGGAGATA GTCCCCGATA GCGAGGAACA GGATAAGGAT 
CAGCAGGAGT CGGCACCCCA AAAGGAACAG CCCGTTGTCC TCGATTCCGG TATTATCGAC 
GAAGAAGAGG ATCAGGAGGA GCAGGAGAAA GAGGAGGAGC ACCAGGACAC CACCACTGCC 
ACCGCCGATT CAATGCGACT CAAACACAGC GCGGACACAG GCATCCCACA ATGGACCGTC 
GAAAGCCATT TGGTCAGTCG CGGCGAACAG CTTAACAATC TCGGCCAGAG CAGCAGCACC 
CAAATCACCG GTCGCAGCAA GGTTCAATCC TCCACAGCCA GCACGGCGAA CGCGAATCCT 
TCGGGATCGG GAGCAACATC ATCCGCTCCG CCCAGCAGCA TTAATATCAT GAACGCCTGG 
GAGCAGTTCT ATCAACAAAA CGACGACGGC GACACACCTT TGCACCTGGC CTGTATTTCG 
GGATCAGTGG ACGTGGTGGC CGCCCTGATT CGCATGGCTC CTCATCCGTG CCTGCTCAAC 
ATCCAGAACG ATGTGGCCCA GACACCCCTT CATCTGGCCG CTCTTACTGC CCAGCCGAAC 
ATCATGAGGA TACTTTTACT CGCCGGAGCT GAGCCCACGG TGCGCGATCG TCATGGAAAC 
ACAGCTCTGC ATCTTTCCTG CATCGCCGGA GAGAAACAGT GCGTCCGTGC CTTGACAGAG 
AAGTTCGGCG CCACGGAAAT CCATGAGGCC CACCGACAAT ATGGACACCG ATCCAACGAC 
AAAGCGGTCA GTTCCCTGAG CTACGCCTGC CTGCCCGCCG ATCTGGAGAT TCGCAACTAC 
GATGGAGAAC GCTGTGTGCA TTTGGCCGCC GAGGCGGGTC ACATTGATAT CTTACGCATT 
TTGGTATCCC ATGGAGCAGA CATTAACGCC AGGGAGGGAA AATCGGGCCG CACACCGCTG 
CACATTGCGA TCGAGGGCTG CAACGAGGAT CTGGCCAACT TCCTGCTCGA TGAGTGCGAG 
AAGCTGAACC TGGAAACGGC CACCTACGCC GGCTTGACGG CCTACCAGTT TGCGTGCATC 
ATGAACAAGT CGCGCATGCA GAACATCCTG GAGAAGCGCG GAGCGGAGAC CGTAACGCCT 
CCGGACAGTG ATTACGACAG TAGCGATATC GAAGATCTAG ACGATACGAA GATGTACGAT 
CGCTTTGGTG ATCCTCGCTA TTTTGTCAGC TACAACGGTG GCAATCCCAT GACAGTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cact ( NM_165152.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723960.1 Drosophila melanogaster cactus (cact), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14736
Clone ID Related Accession (Same CDS sequence)	NM_205999.2 , NM_001273567.1 , NM_165152.2 , NM_057594.4
Accession Version	NM_165152.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cactus, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165152.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAGCC CAACAAAAGC AGCGGAGGCA GCAACAAAGG CAACAGCAAC AAGTGACTGC 
AGCTGCAGCG CTGCCAGTGT TGAGCAGCGC GCCCCCTCAA ACGCGGCTAA CCCCTCGTCC 
TCACTAGCCA CTAGCGGTAA AATTGGCGGC AAAACACAAG ATCAAACGGC CGCCATTAAC 
AAGCAAAAGG AATTTGCCGT GCCAAACGAA ACCAGTGATT CGGGCTTCAT CTCCGGCCCG 
CAATCCTCGC AGATCTTCAG CGAGGAGATA GTCCCCGATA GCGAGGAACA GGATAAGGAT 
CAGCAGGAGT CGGCACCCCA AAAGGAACAG CCCGTTGTCC TCGATTCCGG TATTATCGAC 
GAAGAAGAGG ATCAGGAGGA GCAGGAGAAA GAGGAGGAGC ACCAGGACAC CACCACTGCC 
ACCGCCGATT CAATGCGACT CAAACACAGC GCGGACACAG GCATCCCACA ATGGACCGTC 
GAAAGCCATT TGGTCAGTCG CGGCGAACAG CTTAACAATC TCGGCCAGAG CAGCAGCACC 
CAAATCACCG GTCGCAGCAA GGTTCAATCC TCCACAGCCA GCACGGCGAA CGCGAATCCT 
TCGGGATCGG GAGCAACATC ATCCGCTCCG CCCAGCAGCA TTAATATCAT GAACGCCTGG 
GAGCAGTTCT ATCAACAAAA CGACGACGGC GACACACCTT TGCACCTGGC CTGTATTTCG 
GGATCAGTGG ACGTGGTGGC CGCCCTGATT CGCATGGCTC CTCATCCGTG CCTGCTCAAC 
ATCCAGAACG ATGTGGCCCA GACACCCCTT CATCTGGCCG CTCTTACTGC CCAGCCGAAC 
ATCATGAGGA TACTTTTACT CGCCGGAGCT GAGCCCACGG TGCGCGATCG TCATGGAAAC 
ACAGCTCTGC ATCTTTCCTG CATCGCCGGA GAGAAACAGT GCGTCCGTGC CTTGACAGAG 
AAGTTCGGCG CCACGGAAAT CCATGAGGCC CACCGACAAT ATGGACACCG ATCCAACGAC 
AAAGCGGTCA GTTCCCTGAG CTACGCCTGC CTGCCCGCCG ATCTGGAGAT TCGCAACTAC 
GATGGAGAAC GCTGTGTGCA TTTGGCCGCC GAGGCGGGTC ACATTGATAT CTTACGCATT 
TTGGTATCCC ATGGAGCAGA CATTAACGCC AGGGAGGGAA AATCGGGCCG CACACCGCTG 
CACATTGCGA TCGAGGGCTG CAACGAGGAT CTGGCCAACT TCCTGCTCGA TGAGTGCGAG 
AAGCTGAACC TGGAAACGGC CACCTACGCC GGCTTGACGG CCTACCAGTT TGCGTGCATC 
ATGAACAAGT CGCGCATGCA GAACATCCTG GAGAAGCGCG GAGCGGAGAC CGTAACGCCT 
CCGGACAGTG ATTACGACAG TAGCGATATC GAAGATCTAG ACGATACGAA GATGTACGAT 
CGCTTTGGTG ATCCTCGCTA TTTTGTCAGC TACAACGGTG GCAATCCCAT GACAGTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723960.1
CDS	109..1611
Translation	MPSPTKAAEAATKATATSDCSCSAASVEQRAPSNAANPSSSLATSGKIGGKTQDQTAAINKQKEFAVPNETSDSGFISGPQSSQIFSEEIVPDSEEQDKDQQESAPQKEQPVVLDSGIIDEEEDQEEQEKEEEHQDTTTATADSMRLKHSADTGIPQWTVESHLVSRGEQLNNLGQSSSTQITGRSKVQSSTASTANANPSGSGATSSAPPSSINIMNAWEQFYQQNDDGDTPLHLACISGSVDVVAALIRMAPHPCLLNIQNDVAQTPLHLAALTAQPNIMRILLLAGAEPTVRDRHGNTALHLSCIAGEKQCVRALTEKFGATEIHEAHRQYGHRSNDKAVSSLSYACLPADLEIRNYDGERCVHLAAEAGHIDILRILVSHGADINAREGKSGRTPLHIAIEGCNEDLANFLLDECEKLNLETATYAGLTAYQFACIMNKSRMQNILEKRGAETVTPPDSDYDSSDIEDLDDTKMYDRFGDPRYFVSYNGGNPMTVA

Target ORF information:

RefSeq Version	NM_165152.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cactus (cact), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165152.2

ORF Insert Sequence:

ATGCCGAGCC CAACAAAAGC AGCGGAGGCA GCAACAAAGG CAACAGCAAC AAGTGACTGC 
AGCTGCAGCG CTGCCAGTGT TGAGCAGCGC GCCCCCTCAA ACGCGGCTAA CCCCTCGTCC 
TCACTAGCCA CTAGCGGTAA AATTGGCGGC AAAACACAAG ATCAAACGGC CGCCATTAAC 
AAGCAAAAGG AATTTGCCGT GCCAAACGAA ACCAGTGATT CGGGCTTCAT CTCCGGCCCG 
CAATCCTCGC AGATCTTCAG CGAGGAGATA GTCCCCGATA GCGAGGAACA GGATAAGGAT 
CAGCAGGAGT CGGCACCCCA AAAGGAACAG CCCGTTGTCC TCGATTCCGG TATTATCGAC 
GAAGAAGAGG ATCAGGAGGA GCAGGAGAAA GAGGAGGAGC ACCAGGACAC CACCACTGCC 
ACCGCCGATT CAATGCGACT CAAACACAGC GCGGACACAG GCATCCCACA ATGGACCGTC 
GAAAGCCATT TGGTCAGTCG CGGCGAACAG CTTAACAATC TCGGCCAGAG CAGCAGCACC 
CAAATCACCG GTCGCAGCAA GGTTCAATCC TCCACAGCCA GCACGGCGAA CGCGAATCCT 
TCGGGATCGG GAGCAACATC ATCCGCTCCG CCCAGCAGCA TTAATATCAT GAACGCCTGG 
GAGCAGTTCT ATCAACAAAA CGACGACGGC GACACACCTT TGCACCTGGC CTGTATTTCG 
GGATCAGTGG ACGTGGTGGC CGCCCTGATT CGCATGGCTC CTCATCCGTG CCTGCTCAAC 
ATCCAGAACG ATGTGGCCCA GACACCCCTT CATCTGGCCG CTCTTACTGC CCAGCCGAAC 
ATCATGAGGA TACTTTTACT CGCCGGAGCT GAGCCCACGG TGCGCGATCG TCATGGAAAC 
ACAGCTCTGC ATCTTTCCTG CATCGCCGGA GAGAAACAGT GCGTCCGTGC CTTGACAGAG 
AAGTTCGGCG CCACGGAAAT CCATGAGGCC CACCGACAAT ATGGACACCG ATCCAACGAC 
AAAGCGGTCA GTTCCCTGAG CTACGCCTGC CTGCCCGCCG ATCTGGAGAT TCGCAACTAC 
GATGGAGAAC GCTGTGTGCA TTTGGCCGCC GAGGCGGGTC ACATTGATAT CTTACGCATT 
TTGGTATCCC ATGGAGCAGA CATTAACGCC AGGGAGGGAA AATCGGGCCG CACACCGCTG 
CACATTGCGA TCGAGGGCTG CAACGAGGAT CTGGCCAACT TCCTGCTCGA TGAGTGCGAG 
AAGCTGAACC TGGAAACGGC CACCTACGCC GGCTTGACGG CCTACCAGTT TGCGTGCATC 
ATGAACAAGT CGCGCATGCA GAACATCCTG GAGAAGCGCG GAGCGGAGAC CGTAACGCCT 
CCGGACAGTG ATTACGACAG TAGCGATATC GAAGATCTAG ACGATACGAA GATGTACGAT 
CGCTTTGGTG ATCCTCGCTA TTTTGTCAGC TACAACGGTG GCAATCCCAT GACAGTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cact ( NM_057594.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476942.1 Drosophila melanogaster cactus (cact), transcript variant B, mRNA.

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CloneID	OFa14736
Clone ID Related Accession (Same CDS sequence)	NM_205999.2 , NM_001273567.1 , NM_165152.2 , NM_057594.4
Accession Version	NM_057594.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cactus, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057594.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAGCC CAACAAAAGC AGCGGAGGCA GCAACAAAGG CAACAGCAAC AAGTGACTGC 
AGCTGCAGCG CTGCCAGTGT TGAGCAGCGC GCCCCCTCAA ACGCGGCTAA CCCCTCGTCC 
TCACTAGCCA CTAGCGGTAA AATTGGCGGC AAAACACAAG ATCAAACGGC CGCCATTAAC 
AAGCAAAAGG AATTTGCCGT GCCAAACGAA ACCAGTGATT CGGGCTTCAT CTCCGGCCCG 
CAATCCTCGC AGATCTTCAG CGAGGAGATA GTCCCCGATA GCGAGGAACA GGATAAGGAT 
CAGCAGGAGT CGGCACCCCA AAAGGAACAG CCCGTTGTCC TCGATTCCGG TATTATCGAC 
GAAGAAGAGG ATCAGGAGGA GCAGGAGAAA GAGGAGGAGC ACCAGGACAC CACCACTGCC 
ACCGCCGATT CAATGCGACT CAAACACAGC GCGGACACAG GCATCCCACA ATGGACCGTC 
GAAAGCCATT TGGTCAGTCG CGGCGAACAG CTTAACAATC TCGGCCAGAG CAGCAGCACC 
CAAATCACCG GTCGCAGCAA GGTTCAATCC TCCACAGCCA GCACGGCGAA CGCGAATCCT 
TCGGGATCGG GAGCAACATC ATCCGCTCCG CCCAGCAGCA TTAATATCAT GAACGCCTGG 
GAGCAGTTCT ATCAACAAAA CGACGACGGC GACACACCTT TGCACCTGGC CTGTATTTCG 
GGATCAGTGG ACGTGGTGGC CGCCCTGATT CGCATGGCTC CTCATCCGTG CCTGCTCAAC 
ATCCAGAACG ATGTGGCCCA GACACCCCTT CATCTGGCCG CTCTTACTGC CCAGCCGAAC 
ATCATGAGGA TACTTTTACT CGCCGGAGCT GAGCCCACGG TGCGCGATCG TCATGGAAAC 
ACAGCTCTGC ATCTTTCCTG CATCGCCGGA GAGAAACAGT GCGTCCGTGC CTTGACAGAG 
AAGTTCGGCG CCACGGAAAT CCATGAGGCC CACCGACAAT ATGGACACCG ATCCAACGAC 
AAAGCGGTCA GTTCCCTGAG CTACGCCTGC CTGCCCGCCG ATCTGGAGAT TCGCAACTAC 
GATGGAGAAC GCTGTGTGCA TTTGGCCGCC GAGGCGGGTC ACATTGATAT CTTACGCATT 
TTGGTATCCC ATGGAGCAGA CATTAACGCC AGGGAGGGAA AATCGGGCCG CACACCGCTG 
CACATTGCGA TCGAGGGCTG CAACGAGGAT CTGGCCAACT TCCTGCTCGA TGAGTGCGAG 
AAGCTGAACC TGGAAACGGC CACCTACGCC GGCTTGACGG CCTACCAGTT TGCGTGCATC 
ATGAACAAGT CGCGCATGCA GAACATCCTG GAGAAGCGCG GAGCGGAGAC CGTAACGCCT 
CCGGACAGTG ATTACGACAG TAGCGATATC GAAGATCTAG ACGATACGAA GATGTACGAT 
CGCTTTGGTG ATCCTCGCTA TTTTGTCAGC TACAACGGTG GCAATCCCAT GACAGTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476942.1
CDS	160..1662
Translation	MPSPTKAAEAATKATATSDCSCSAASVEQRAPSNAANPSSSLATSGKIGGKTQDQTAAINKQKEFAVPNETSDSGFISGPQSSQIFSEEIVPDSEEQDKDQQESAPQKEQPVVLDSGIIDEEEDQEEQEKEEEHQDTTTATADSMRLKHSADTGIPQWTVESHLVSRGEQLNNLGQSSSTQITGRSKVQSSTASTANANPSGSGATSSAPPSSINIMNAWEQFYQQNDDGDTPLHLACISGSVDVVAALIRMAPHPCLLNIQNDVAQTPLHLAALTAQPNIMRILLLAGAEPTVRDRHGNTALHLSCIAGEKQCVRALTEKFGATEIHEAHRQYGHRSNDKAVSSLSYACLPADLEIRNYDGERCVHLAAEAGHIDILRILVSHGADINAREGKSGRTPLHIAIEGCNEDLANFLLDECEKLNLETATYAGLTAYQFACIMNKSRMQNILEKRGAETVTPPDSDYDSSDIEDLDDTKMYDRFGDPRYFVSYNGGNPMTVA

Target ORF information:

RefSeq Version	NM_057594.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cactus (cact), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057594.4

ORF Insert Sequence:

ATGCCGAGCC CAACAAAAGC AGCGGAGGCA GCAACAAAGG CAACAGCAAC AAGTGACTGC 
AGCTGCAGCG CTGCCAGTGT TGAGCAGCGC GCCCCCTCAA ACGCGGCTAA CCCCTCGTCC 
TCACTAGCCA CTAGCGGTAA AATTGGCGGC AAAACACAAG ATCAAACGGC CGCCATTAAC 
AAGCAAAAGG AATTTGCCGT GCCAAACGAA ACCAGTGATT CGGGCTTCAT CTCCGGCCCG 
CAATCCTCGC AGATCTTCAG CGAGGAGATA GTCCCCGATA GCGAGGAACA GGATAAGGAT 
CAGCAGGAGT CGGCACCCCA AAAGGAACAG CCCGTTGTCC TCGATTCCGG TATTATCGAC 
GAAGAAGAGG ATCAGGAGGA GCAGGAGAAA GAGGAGGAGC ACCAGGACAC CACCACTGCC 
ACCGCCGATT CAATGCGACT CAAACACAGC GCGGACACAG GCATCCCACA ATGGACCGTC 
GAAAGCCATT TGGTCAGTCG CGGCGAACAG CTTAACAATC TCGGCCAGAG CAGCAGCACC 
CAAATCACCG GTCGCAGCAA GGTTCAATCC TCCACAGCCA GCACGGCGAA CGCGAATCCT 
TCGGGATCGG GAGCAACATC ATCCGCTCCG CCCAGCAGCA TTAATATCAT GAACGCCTGG 
GAGCAGTTCT ATCAACAAAA CGACGACGGC GACACACCTT TGCACCTGGC CTGTATTTCG 
GGATCAGTGG ACGTGGTGGC CGCCCTGATT CGCATGGCTC CTCATCCGTG CCTGCTCAAC 
ATCCAGAACG ATGTGGCCCA GACACCCCTT CATCTGGCCG CTCTTACTGC CCAGCCGAAC 
ATCATGAGGA TACTTTTACT CGCCGGAGCT GAGCCCACGG TGCGCGATCG TCATGGAAAC 
ACAGCTCTGC ATCTTTCCTG CATCGCCGGA GAGAAACAGT GCGTCCGTGC CTTGACAGAG 
AAGTTCGGCG CCACGGAAAT CCATGAGGCC CACCGACAAT ATGGACACCG ATCCAACGAC 
AAAGCGGTCA GTTCCCTGAG CTACGCCTGC CTGCCCGCCG ATCTGGAGAT TCGCAACTAC 
GATGGAGAAC GCTGTGTGCA TTTGGCCGCC GAGGCGGGTC ACATTGATAT CTTACGCATT 
TTGGTATCCC ATGGAGCAGA CATTAACGCC AGGGAGGGAA AATCGGGCCG CACACCGCTG 
CACATTGCGA TCGAGGGCTG CAACGAGGAT CTGGCCAACT TCCTGCTCGA TGAGTGCGAG 
AAGCTGAACC TGGAAACGGC CACCTACGCC GGCTTGACGG CCTACCAGTT TGCGTGCATC 
ATGAACAAGT CGCGCATGCA GAACATCCTG GAGAAGCGCG GAGCGGAGAC CGTAACGCCT 
CCGGACAGTG ATTACGACAG TAGCGATATC GAAGATCTAG ACGATACGAA GATGTACGAT 
CGCTTTGGTG ATCCTCGCTA TTTTGTCAGC TACAACGGTG GCAATCCCAT GACAGTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cact ( NM_057595.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476943.1 Drosophila melanogaster cactus (cact), transcript variant C, mRNA.

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CloneID	OFa16831
Clone ID Related Accession (Same CDS sequence)	NM_057595.4
Accession Version	NM_057595.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cactus, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057595.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAGCC CAACAAAAGC AGCGGAGGCA GCAACAAAGG CAACAGCAAC AAGTGACTGC 
AGCTGCAGCG CTGCCAGTGT TGAGCAGCGC GCCCCCTCAA ACGCGGCTAA CCCCTCGTCC 
TCACTAGCCA CTAGCGGTAA AATTGGCGGC AAAACACAAG ATCAAACGGC CGCCATTAAC 
AAGCAAAAGG AATTTGCCGT GCCAAACGAA ACCAGTGATT CGGGCTTCAT CTCCGGCCCG 
CAATCCTCGC AGATCTTCAG CGAGGAGATA GTCCCCGATA GCGAGGAACA GGATAAGGAT 
CAGCAGGAGT CGGCACCCCA AAAGGAACAG CCCGTTGTCC TCGATTCCGG TATTATCGAC 
GAAGAAGAGG ATCAGGAGGA GCAGGAGAAA GAGGAGGAGC ACCAGGACAC CACCACTGCC 
ACCGCCGATT CAATGCGACT CAAACACAGC GCGGACACAG GCATCCCACA ATGGACCGTC 
GAAAGCCATT TGGTCAGTCG CGGCGAACAG CTTAACAATC TCGGCCAGAG CAGCAGCACC 
CAAATCACCG GTCGCAGCAA GGTTCAATCC TCCACAGCCA GCACGGCGAA CGCGAATCCT 
TCGGGATCGG GAGCAACATC ATCCGCTCCG CCCAGCAGCA TTAATATCAT GAACGCCTGG 
GAGCAGTTCT ATCAACAAAA CGACGACGGC GACACACCTT TGCACCTGGC CTGTATTTCG 
GGATCAGTGG ACGTGGTGGC CGCCCTGATT CGCATGGCTC CTCATCCGTG CCTGCTCAAC 
ATCCAGAACG ATGTGGCCCA GACACCCCTT CATCTGGCCG CTCTTACTGC CCAGCCGAAC 
ATCATGAGGA TACTTTTACT CGCCGGAGCT GAGCCCACGG TGCGCGATCG TCATGGAAAC 
ACAGCTCTGC ATCTTTCCTG CATCGCCGGA GAGAAACAGT GCGTCCGTGC CTTGACAGAG 
AAGTTCGGCG CCACGGAAAT CCATGAGGCC CACCGACAAT ATGGACACCG ATCCAACGAC 
AAAGCGGTCA GTTCCCTGAG CTACGCCTGC CTGCCCGCCG ATCTGGAGAT TCGCAACTAC 
GATGGAGAAC GCTGTGTGCA TTTGGCCGCC GAGGCGGGTC ACATTGATAT CTTACGCATT 
TTGGTATCCC ATGGAGCAGA CATTAACGCC AGGGAGGGAA AATCGGGCCG CACACCGCTG 
CACATTGCGA TCGAGGGCTG CAACGAGGAT CTGGCCAACT TCCTGCTCGA TGAGTGCGAG 
AAGCTGAACC TGGAAACGGC CACCTACGCC GGCTTGACGG CCTACCAGTT TGCGTGCATC 
ATGAACAAGT CGCGCATGCA GAACATCCTG GAGAAGCGCG GAGCGGAGAC CGTAACGCCT 
CCGGACAGTG ATTACGACAG TAGCGATATC GAAGATCTAG ACGATACGAA GGTGAGCGTC 
ACTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476943.1
CDS	160..1608
Translation	MPSPTKAAEAATKATATSDCSCSAASVEQRAPSNAANPSSSLATSGKIGGKTQDQTAAINKQKEFAVPNETSDSGFISGPQSSQIFSEEIVPDSEEQDKDQQESAPQKEQPVVLDSGIIDEEEDQEEQEKEEEHQDTTTATADSMRLKHSADTGIPQWTVESHLVSRGEQLNNLGQSSSTQITGRSKVQSSTASTANANPSGSGATSSAPPSSINIMNAWEQFYQQNDDGDTPLHLACISGSVDVVAALIRMAPHPCLLNIQNDVAQTPLHLAALTAQPNIMRILLLAGAEPTVRDRHGNTALHLSCIAGEKQCVRALTEKFGATEIHEAHRQYGHRSNDKAVSSLSYACLPADLEIRNYDGERCVHLAAEAGHIDILRILVSHGADINAREGKSGRTPLHIAIEGCNEDLANFLLDECEKLNLETATYAGLTAYQFACIMNKSRMQNILEKRGAETVTPPDSDYDSSDIEDLDDTKVSVTA

Target ORF information:

RefSeq Version	NM_057595.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cactus (cact), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057595.4

ORF Insert Sequence:

ATGCCGAGCC CAACAAAAGC AGCGGAGGCA GCAACAAAGG CAACAGCAAC AAGTGACTGC 
AGCTGCAGCG CTGCCAGTGT TGAGCAGCGC GCCCCCTCAA ACGCGGCTAA CCCCTCGTCC 
TCACTAGCCA CTAGCGGTAA AATTGGCGGC AAAACACAAG ATCAAACGGC CGCCATTAAC 
AAGCAAAAGG AATTTGCCGT GCCAAACGAA ACCAGTGATT CGGGCTTCAT CTCCGGCCCG 
CAATCCTCGC AGATCTTCAG CGAGGAGATA GTCCCCGATA GCGAGGAACA GGATAAGGAT 
CAGCAGGAGT CGGCACCCCA AAAGGAACAG CCCGTTGTCC TCGATTCCGG TATTATCGAC 
GAAGAAGAGG ATCAGGAGGA GCAGGAGAAA GAGGAGGAGC ACCAGGACAC CACCACTGCC 
ACCGCCGATT CAATGCGACT CAAACACAGC GCGGACACAG GCATCCCACA ATGGACCGTC 
GAAAGCCATT TGGTCAGTCG CGGCGAACAG CTTAACAATC TCGGCCAGAG CAGCAGCACC 
CAAATCACCG GTCGCAGCAA GGTTCAATCC TCCACAGCCA GCACGGCGAA CGCGAATCCT 
TCGGGATCGG GAGCAACATC ATCCGCTCCG CCCAGCAGCA TTAATATCAT GAACGCCTGG 
GAGCAGTTCT ATCAACAAAA CGACGACGGC GACACACCTT TGCACCTGGC CTGTATTTCG 
GGATCAGTGG ACGTGGTGGC CGCCCTGATT CGCATGGCTC CTCATCCGTG CCTGCTCAAC 
ATCCAGAACG ATGTGGCCCA GACACCCCTT CATCTGGCCG CTCTTACTGC CCAGCCGAAC 
ATCATGAGGA TACTTTTACT CGCCGGAGCT GAGCCCACGG TGCGCGATCG TCATGGAAAC 
ACAGCTCTGC ATCTTTCCTG CATCGCCGGA GAGAAACAGT GCGTCCGTGC CTTGACAGAG 
AAGTTCGGCG CCACGGAAAT CCATGAGGCC CACCGACAAT ATGGACACCG ATCCAACGAC 
AAAGCGGTCA GTTCCCTGAG CTACGCCTGC CTGCCCGCCG ATCTGGAGAT TCGCAACTAC 
GATGGAGAAC GCTGTGTGCA TTTGGCCGCC GAGGCGGGTC ACATTGATAT CTTACGCATT 
TTGGTATCCC ATGGAGCAGA CATTAACGCC AGGGAGGGAA AATCGGGCCG CACACCGCTG 
CACATTGCGA TCGAGGGCTG CAACGAGGAT CTGGCCAACT TCCTGCTCGA TGAGTGCGAG 
AAGCTGAACC TGGAAACGGC CACCTACGCC GGCTTGACGG CCTACCAGTT TGCGTGCATC 
ATGAACAAGT CGCGCATGCA GAACATCCTG GAGAAGCGCG GAGCGGAGAC CGTAACGCCT 
CCGGACAGTG ATTACGACAG TAGCGATATC GAAGATCTAG ACGATACGAA GGTGAGCGTC 
ACTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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cact cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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