pkaap cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pkaap
Entrez Gene ID	34957
Full Name	CG4132 gene product from transcript CG4132-RA
Synonyms	BG:DS02740.4,CG4132,DS02740.4,Dmel\CG4132
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35F1-35F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_135939.3	NP_609783.1	pkaap

Pathways from BioSystems

Homologies

Homo sapiens (human)	AKAP10	NP_009133.2
Gallus gallus (chicken)	AKAP10	XP_415856.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007895	XP_317593.4
Mus musculus (house mouse)	Akap10	NP_064305.2
Bos taurus (cattle)	AKAP10	NP_001091493.1
Rattus norvegicus (Norway rat)	Akap10	NP_001108078.1
Drosophila melanogaster (fruit fly)	pkaap	NP_609783.1
Xenopus tropicalis (tropical clawed frog)	akap10	XP_002938286.2
Pan troglodytes (chimpanzee)	AKAP10	XP_003315625.1
	AKAP10	XP_001099682.2
Canis lupus familiaris (dog)	AKAP10	XP_851367.1
Danio rerio (zebrafish)	akap10	XP_005165633.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pkaap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pkaap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16821	NM_135939.3 Latest version!	Drosophila melanogaster pkaap (pkaap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pkaap ( NM_135939.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609783.1 Drosophila melanogaster pkaap (pkaap), mRNA.

Price & Availability↑

CloneID	OFa16821
Clone ID Related Accession (Same CDS sequence)	NM_135939.3
Accession Version	NM_135939.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pkaap
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135939.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAAATT ACATCAAGAG GCACGCCAGC CGACGTCGCA GCAGCCAAGA TGCCACGGAT 
GCCATTTGTG GCCAAATGCC AGCGTATGAT GTGGACACCA ACGACGTTGC TGGAGCTGGT 
GATGCAGCCA ACGCTCACAA ACGACACTCG CTGCGCTCCA CAACCAGTTT CTGCGACGAA 
GTGTTCCTAG AAGCGGAGGA GGAGGGCGGC GTGGGAACCA CAGGAGGAGG AGGAGGAGGA 
GGAGGTGGTG GTGGTGGTGG AGGAGCCCCC TCCGATGGCA TCAGTCTCGC CAGCAACGAT 
GACATTTTCA CCTACAACTC ACGCCTCTCC ATGAATCTCG CTTCCATCGT CAACGATCCG 
AACTGCCTGA GCTACTTTGT TCAGTATCTG GACACCCGAC AGGCCCTGCC GCTGATCAAA 
TTCTACTTGG ACATTGAGAA CTTTAAGCGG GCGGCTCTGA CGCAAGAGAA GGAGGAACCA 
GAGGAACCTC CTCGCAAGGC AATAGAACAA TCTTCTCCGC ACAAAGATGA CAAAGATGTA 
CCTGAATTGA AGACGCTGTG CGATCTGTCA ATGCGCAAAC CCCTCACAGA CGACGAGAAG 
AGCCGCATCT ATGCGGAGAC CAACAAGCAG CTCAATAGGC AAAAGTCTGG AACGGATAGT 
GTGCTGAACG CCGAGCTATC GCGGGCCAGC ATCAGCGATG CCATTGCCAT ATACCAAAAG 
TATCTGATAG TGAACGCCAG CCTGCAAGTG GAGCTGCCCA TCGTCATCCT TGCGCATATA 
TCGCTGCTAC TTTGCGGAAG GGATAAGTCC GAGTGTAGCA AACCAATTCC GGCCAGCTGC 
TTTGACGAAG CTAGAGAGTT TGTGCTGGAG CAGCTGGAGC GGGATCATGT GCAGGGATTT 
CTACAGAGCG GCTACTATTC TACATACTGT CTGGAGTTGA TCGAAGGCGG CTCTGTAAAT 
ATCTACGATG TCTTGAACAG CGAACTGGCT TTGTTCTACT TCACCGAGTA CCTGGAGCAG 
CGACAAGAGC GTGAATGCCT GGAGTTCTGG ATCACCGCCA TTAACTTCCG CAAGAGCTTT 
GCTTGTGGCG AGGAGAAGGA AGAGGATCGC AAGGCTGCGC AAAGCGATGC CATGATCATA 
TACGACAAAT ACTTCTCCCT CCAGTCCGAG TGCCGTCTGT GGATGTCCCA GAAGCTGCGC 
TTACGAGTGG AACAAGCGAT CTGTGCTCCG AATGAGCAGT GGCAAGCCTT CGATTTGGCC 
CTCTTGGTGA CGGCCAAGTA TCTGGAGCAA AAGTATTTCG CTGACTTTCT AAAGTCGCAC 
ATCTTCGATA ACTACGTGAA CGAACTGAAG GTCAAGAGAG ATAATTCCAC CACTCATCCG 
AATCTCCAGC ACACGCTGAG CTTGCGAAGA GCTGGCAAGT CCTCCAATGG CCAACAACAG 
CGACATCGAA AAACTCTATC CATGTCAGAC TGCACGCACA TTTCGCAGCA CAACACACTG 
CTGGCTTCCA TGGATCAGGC GCCGTCCAAA ACAGCGCTAA ATCATCAGTC GGGAAATCTC 
AACATCGATG CTAGGCAGCT AACGAATCCA CAACTTTTGT GGCAGCGTCC TGTGGGCGCA 
CTGAAATTCG GTTTTGTTAA CTCCTTGGGA CGCTACGAGC GGGATTTTGA GGCCGTGGAC 
GCAGTGGCTC TGAAATCGCA GCCGTGGTCA CTGAGCGTCA GCGGTAGCAA GCTGAAAAAT 
GCCATGCGAA AGCTGGTGAA CCTGCCGGAG GATAATGTGC AAGAGGAGAT AGCCTGGCAG 
GTGGCCGAAA TGATTGTCCG GGATGTTACG AGTGTCACTC TTAGTGGCAA GACTCCTCCG 
CTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609783.1
CDS	155..2023
Translation	MLNYIKRHASRRRSSQDATDAICGQMPAYDVDTNDVAGAGDAANAHKRHSLRSTTSFCDEVFLEAEEEGGVGTTGGGGGGGGGGGGGGAPSDGISLASNDDIFTYNSRLSMNLASIVNDPNCLSYFVQYLDTRQALPLIKFYLDIENFKRAALTQEKEEPEEPPRKAIEQSSPHKDDKDVPELKTLCDLSMRKPLTDDEKSRIYAETNKQLNRQKSGTDSVLNAELSRASISDAIAIYQKYLIVNASLQVELPIVILAHISLLLCGRDKSECSKPIPASCFDEAREFVLEQLERDHVQGFLQSGYYSTYCLELIEGGSVNIYDVLNSELALFYFTEYLEQRQERECLEFWITAINFRKSFACGEEKEEDRKAAQSDAMIIYDKYFSLQSECRLWMSQKLRLRVEQAICAPNEQWQAFDLALLVTAKYLEQKYFADFLKSHIFDNYVNELKVKRDNSTTHPNLQHTLSLRRAGKSSNGQQQRHRKTLSMSDCTHISQHNTLLASMDQAPSKTALNHQSGNLNIDARQLTNPQLLWQRPVGALKFGFVNSLGRYERDFEAVDAVALKSQPWSLSVSGSKLKNAMRKLVNLPEDNVQEEIAWQVAEMIVRDVTSVTLSGKTPPLA

Target ORF information:

RefSeq Version	NM_135939.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pkaap (pkaap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135939.3

ORF Insert Sequence:

ATGTTAAATT ACATCAAGAG GCACGCCAGC CGACGTCGCA GCAGCCAAGA TGCCACGGAT 
GCCATTTGTG GCCAAATGCC AGCGTATGAT GTGGACACCA ACGACGTTGC TGGAGCTGGT 
GATGCAGCCA ACGCTCACAA ACGACACTCG CTGCGCTCCA CAACCAGTTT CTGCGACGAA 
GTGTTCCTAG AAGCGGAGGA GGAGGGCGGC GTGGGAACCA CAGGAGGAGG AGGAGGAGGA 
GGAGGTGGTG GTGGTGGTGG AGGAGCCCCC TCCGATGGCA TCAGTCTCGC CAGCAACGAT 
GACATTTTCA CCTACAACTC ACGCCTCTCC ATGAATCTCG CTTCCATCGT CAACGATCCG 
AACTGCCTGA GCTACTTTGT TCAGTATCTG GACACCCGAC AGGCCCTGCC GCTGATCAAA 
TTCTACTTGG ACATTGAGAA CTTTAAGCGG GCGGCTCTGA CGCAAGAGAA GGAGGAACCA 
GAGGAACCTC CTCGCAAGGC AATAGAACAA TCTTCTCCGC ACAAAGATGA CAAAGATGTA 
CCTGAATTGA AGACGCTGTG CGATCTGTCA ATGCGCAAAC CCCTCACAGA CGACGAGAAG 
AGCCGCATCT ATGCGGAGAC CAACAAGCAG CTCAATAGGC AAAAGTCTGG AACGGATAGT 
GTGCTGAACG CCGAGCTATC GCGGGCCAGC ATCAGCGATG CCATTGCCAT ATACCAAAAG 
TATCTGATAG TGAACGCCAG CCTGCAAGTG GAGCTGCCCA TCGTCATCCT TGCGCATATA 
TCGCTGCTAC TTTGCGGAAG GGATAAGTCC GAGTGTAGCA AACCAATTCC GGCCAGCTGC 
TTTGACGAAG CTAGAGAGTT TGTGCTGGAG CAGCTGGAGC GGGATCATGT GCAGGGATTT 
CTACAGAGCG GCTACTATTC TACATACTGT CTGGAGTTGA TCGAAGGCGG CTCTGTAAAT 
ATCTACGATG TCTTGAACAG CGAACTGGCT TTGTTCTACT TCACCGAGTA CCTGGAGCAG 
CGACAAGAGC GTGAATGCCT GGAGTTCTGG ATCACCGCCA TTAACTTCCG CAAGAGCTTT 
GCTTGTGGCG AGGAGAAGGA AGAGGATCGC AAGGCTGCGC AAAGCGATGC CATGATCATA 
TACGACAAAT ACTTCTCCCT CCAGTCCGAG TGCCGTCTGT GGATGTCCCA GAAGCTGCGC 
TTACGAGTGG AACAAGCGAT CTGTGCTCCG AATGAGCAGT GGCAAGCCTT CGATTTGGCC 
CTCTTGGTGA CGGCCAAGTA TCTGGAGCAA AAGTATTTCG CTGACTTTCT AAAGTCGCAC 
ATCTTCGATA ACTACGTGAA CGAACTGAAG GTCAAGAGAG ATAATTCCAC CACTCATCCG 
AATCTCCAGC ACACGCTGAG CTTGCGAAGA GCTGGCAAGT CCTCCAATGG CCAACAACAG 
CGACATCGAA AAACTCTATC CATGTCAGAC TGCACGCACA TTTCGCAGCA CAACACACTG 
CTGGCTTCCA TGGATCAGGC GCCGTCCAAA ACAGCGCTAA ATCATCAGTC GGGAAATCTC 
AACATCGATG CTAGGCAGCT AACGAATCCA CAACTTTTGT GGCAGCGTCC TGTGGGCGCA 
CTGAAATTCG GTTTTGTTAA CTCCTTGGGA CGCTACGAGC GGGATTTTGA GGCCGTGGAC 
GCAGTGGCTC TGAAATCGCA GCCGTGGTCA CTGAGCGTCA GCGGTAGCAA GCTGAAAAAT 
GCCATGCGAA AGCTGGTGAA CCTGCCGGAG GATAATGTGC AAGAGGAGAT AGCCTGGCAG 
GTGGCCGAAA TGATTGTCCG GGATGTTACG AGTGTCACTC TTAGTGGCAA GACTCCTCCG 
CTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pkaap cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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