twe cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	twe
Entrez Gene ID	34954
Full Name	twine
Synonyms	BG:DS02740.1,CG4965,Cdc25,Dmel\CG4965,TWINE,Twe,cdc25,l(2)35Fh,mat(2)syn-A,mat(2)synHB5,mat(2)syn[HB5],twn
General protein information	Preferred Names twine Names CG4965 gene product from transcript CG4965-RA CG4965-PA CG4965-PB CG4965-PC CG4965-PD twe-PA twe-PB twe-PC twe-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35F1-35F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057285.4	NP_476633.1	twine, isoform A
NM_001273564.2	NP_001260493.1	twine, isoform D
NM_001273562.2	NP_001260491.1	twine, isoform B
NM_001273563.1	NP_001260492.1	twine, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

developmentally regulated destruction of Twine protein is a critical switch that contributes to the cell-cycle change at the midblastula transition
Title: Mechanism and regulation of Cdc25/Twine protein destruction in embryonic cell-cycle remodeling.

Posttranslational control of Twine degradation ensures that the proper number of mitoses precede the midblastula transition.
Title: Posttranslational control of Cdc25 degradation terminates Drosophila's early cell-cycle program.

The following twe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the twe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14732	NM_001273564.2 Latest version!	Drosophila melanogaster twine (twe), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14732	NM_057285.4 Latest version!	Drosophila melanogaster twine (twe), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14732	NM_001273563.1 Latest version!	Drosophila melanogaster twine (twe), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14732	NM_001273562.2 Latest version!	Drosophila melanogaster twine (twe), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

twe ( NM_057285.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476633.1 Drosophila melanogaster twine (twe), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14732
Clone ID Related Accession (Same CDS sequence)	NM_057285.4 , NM_001273564.2 , NM_001273562.2 , NM_001273563.1
Accession Version	NM_057285.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twine, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057285.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA 
TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC 
ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC 
ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG 
TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG 
CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG 
TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG 
GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG 
CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA 
CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG 
TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT 
CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT 
GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC 
CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG 
TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC 
TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT 
ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG 
GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA 
CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC 
GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA 
CGACTGCTCT ACGCCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476633.1
CDS	288..1568
Translation	MASKRLMLDVEEEDDESGACGQENFDPHDADMEYQAKRRKSAVQETPLQWMLKRHIPASTTVLSPITELSQNMNGARLDGTPKSTQRIPANRTLNNFNSLSSRTLGSFSSSCSSYESGNSLDDEYMDMFEMESAENHNLELPDDLEVLLSGQLKSESNLEEMSNKKGSLRRCLSMYPSEQPEEAVQEPDQETNMPMKKMQRKTLSMNDAEIMRALGDEPELIGDLSKPCTLPCLATGIRHRDLKTISSDTLARLIQGEFDEQLGSQGGYEIIDCRYPYEFLGGHIRGAKNLYTRGQIQEAFPTLTSNQENRRIYVFHCEFSSERGPKLLRYLRSNDRSQHTHNYPALDYPELYILHNGYKEFFGLYSQLCQPSQYVPMLAPAHNDEFRYFRAKTKSWQCGEGGDSGIGGGGSRGLRKSRSRLLYAE

Target ORF information:

RefSeq Version	NM_057285.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twine (twe), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057285.4

ORF Insert Sequence:

ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA 
TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC 
ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC 
ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG 
TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG 
CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG 
TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG 
GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG 
CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA 
CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG 
TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT 
CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT 
GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC 
CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG 
TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC 
TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT 
ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG 
GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA 
CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC 
GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA 
CGACTGCTCT ACGCCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twe ( NM_001273564.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260493.1 Drosophila melanogaster twine (twe), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa14732
Clone ID Related Accession (Same CDS sequence)	NM_057285.4 , NM_001273564.2 , NM_001273562.2 , NM_001273563.1
Accession Version	NM_001273564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twine, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273564.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA 
TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC 
ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC 
ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG 
TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG 
CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG 
TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG 
GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG 
CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA 
CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG 
TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT 
CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT 
GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC 
CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG 
TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC 
TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT 
ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG 
GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA 
CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC 
GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA 
CGACTGCTCT ACGCCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260493.1
CDS	288..1568
Translation	MASKRLMLDVEEEDDESGACGQENFDPHDADMEYQAKRRKSAVQETPLQWMLKRHIPASTTVLSPITELSQNMNGARLDGTPKSTQRIPANRTLNNFNSLSSRTLGSFSSSCSSYESGNSLDDEYMDMFEMESAENHNLELPDDLEVLLSGQLKSESNLEEMSNKKGSLRRCLSMYPSEQPEEAVQEPDQETNMPMKKMQRKTLSMNDAEIMRALGDEPELIGDLSKPCTLPCLATGIRHRDLKTISSDTLARLIQGEFDEQLGSQGGYEIIDCRYPYEFLGGHIRGAKNLYTRGQIQEAFPTLTSNQENRRIYVFHCEFSSERGPKLLRYLRSNDRSQHTHNYPALDYPELYILHNGYKEFFGLYSQLCQPSQYVPMLAPAHNDEFRYFRAKTKSWQCGEGGDSGIGGGGSRGLRKSRSRLLYAE

Target ORF information:

RefSeq Version	NM_001273564.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twine (twe), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273564.2

ORF Insert Sequence:

ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA 
TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC 
ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC 
ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG 
TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG 
CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG 
TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG 
GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG 
CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA 
CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG 
TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT 
CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT 
GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC 
CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG 
TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC 
TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT 
ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG 
GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA 
CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC 
GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA 
CGACTGCTCT ACGCCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twe ( NM_001273562.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260491.1 Drosophila melanogaster twine (twe), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14732
Clone ID Related Accession (Same CDS sequence)	NM_057285.4 , NM_001273564.2 , NM_001273562.2 , NM_001273563.1
Accession Version	NM_001273562.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twine, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273562.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA 
TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC 
ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC 
ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG 
TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG 
CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG 
TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG 
GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG 
CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA 
CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG 
TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT 
CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT 
GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC 
CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG 
TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC 
TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT 
ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG 
GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA 
CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC 
GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA 
CGACTGCTCT ACGCCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260491.1
CDS	175..1455
Translation	MASKRLMLDVEEEDDESGACGQENFDPHDADMEYQAKRRKSAVQETPLQWMLKRHIPASTTVLSPITELSQNMNGARLDGTPKSTQRIPANRTLNNFNSLSSRTLGSFSSSCSSYESGNSLDDEYMDMFEMESAENHNLELPDDLEVLLSGQLKSESNLEEMSNKKGSLRRCLSMYPSEQPEEAVQEPDQETNMPMKKMQRKTLSMNDAEIMRALGDEPELIGDLSKPCTLPCLATGIRHRDLKTISSDTLARLIQGEFDEQLGSQGGYEIIDCRYPYEFLGGHIRGAKNLYTRGQIQEAFPTLTSNQENRRIYVFHCEFSSERGPKLLRYLRSNDRSQHTHNYPALDYPELYILHNGYKEFFGLYSQLCQPSQYVPMLAPAHNDEFRYFRAKTKSWQCGEGGDSGIGGGGSRGLRKSRSRLLYAE

Target ORF information:

RefSeq Version	NM_001273562.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twine (twe), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273562.2

ORF Insert Sequence:

ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA 
TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC 
ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC 
ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG 
TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG 
CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG 
TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG 
GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG 
CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA 
CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG 
TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT 
CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT 
GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC 
CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG 
TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC 
TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT 
ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG 
GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA 
CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC 
GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA 
CGACTGCTCT ACGCCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twe ( NM_001273563.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260492.1 Drosophila melanogaster twine (twe), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa14732
Clone ID Related Accession (Same CDS sequence)	NM_057285.4 , NM_001273564.2 , NM_001273562.2 , NM_001273563.1
Accession Version	NM_001273563.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twine, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA 
TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC 
ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC 
ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG 
TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG 
CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG 
TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG 
GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG 
CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA 
CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG 
TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT 
CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT 
GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC 
CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG 
TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC 
TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT 
ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG 
GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA 
CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC 
GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA 
CGACTGCTCT ACGCCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260492.1
CDS	149..1429
Translation	MASKRLMLDVEEEDDESGACGQENFDPHDADMEYQAKRRKSAVQETPLQWMLKRHIPASTTVLSPITELSQNMNGARLDGTPKSTQRIPANRTLNNFNSLSSRTLGSFSSSCSSYESGNSLDDEYMDMFEMESAENHNLELPDDLEVLLSGQLKSESNLEEMSNKKGSLRRCLSMYPSEQPEEAVQEPDQETNMPMKKMQRKTLSMNDAEIMRALGDEPELIGDLSKPCTLPCLATGIRHRDLKTISSDTLARLIQGEFDEQLGSQGGYEIIDCRYPYEFLGGHIRGAKNLYTRGQIQEAFPTLTSNQENRRIYVFHCEFSSERGPKLLRYLRSNDRSQHTHNYPALDYPELYILHNGYKEFFGLYSQLCQPSQYVPMLAPAHNDEFRYFRAKTKSWQCGEGGDSGIGGGGSRGLRKSRSRLLYAE

Target ORF information:

RefSeq Version	NM_001273563.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twine (twe), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273563.1

ORF Insert Sequence:

ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA 
TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC 
ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC 
ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG 
TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG 
CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG 
TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG 
GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG 
CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA 
CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG 
TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT 
CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT 
GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC 
CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG 
TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC 
TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT 
ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG 
GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA 
CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC 
GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA 
CGACTGCTCT ACGCCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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twe cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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