twe cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following twe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the twe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa14732 NM_001273564.2
Latest version!
Drosophila melanogaster twine (twe), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OFa14732 NM_057285.4
Latest version!
Drosophila melanogaster twine (twe), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OFa14732 NM_001273563.1
Latest version!
Drosophila melanogaster twine (twe), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OFa14732 NM_001273562.2
Latest version!
Drosophila melanogaster twine (twe), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa14732
    Clone ID Related Accession (Same CDS sequence) NM_057285.4 , NM_001273564.2 , NM_001273562.2 , NM_001273563.1
    Accession Version NM_057285.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twine, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057285.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
    GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA
    TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC
    ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC
    ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG
    TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG
    CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG
    TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG
    GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG
    CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA
    CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG
    TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT
    CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT
    GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC
    CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG
    TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC
    TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT
    ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG
    GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA
    CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC
    GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA
    CGACTGCTCT ACGCCGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_476633.1
    CDS288..1568
    Translation

    Target ORF information:

    RefSeq Version NM_057285.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twine (twe), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057285.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
    GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA
    TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC
    ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC
    ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG
    TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG
    CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG
    TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG
    GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG
    CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA
    CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG
    TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT
    CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT
    GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC
    CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG
    TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC
    TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT
    ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG
    GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA
    CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC
    GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA
    CGACTGCTCT ACGCCGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14732
    Clone ID Related Accession (Same CDS sequence) NM_057285.4 , NM_001273564.2 , NM_001273562.2 , NM_001273563.1
    Accession Version NM_001273564.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twine, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273564.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
    GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA
    TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC
    ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC
    ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG
    TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG
    CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG
    TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG
    GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG
    CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA
    CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG
    TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT
    CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT
    GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC
    CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG
    TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC
    TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT
    ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG
    GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA
    CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC
    GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA
    CGACTGCTCT ACGCCGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260493.1
    CDS288..1568
    Translation

    Target ORF information:

    RefSeq Version NM_001273564.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twine (twe), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273564.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
    GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA
    TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC
    ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC
    ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG
    TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG
    CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG
    TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG
    GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG
    CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA
    CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG
    TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT
    CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT
    GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC
    CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG
    TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC
    TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT
    ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG
    GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA
    CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC
    GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA
    CGACTGCTCT ACGCCGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14732
    Clone ID Related Accession (Same CDS sequence) NM_057285.4 , NM_001273564.2 , NM_001273562.2 , NM_001273563.1
    Accession Version NM_001273562.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twine, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273562.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
    GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA
    TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC
    ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC
    ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG
    TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG
    CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG
    TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG
    GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG
    CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA
    CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG
    TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT
    CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT
    GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC
    CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG
    TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC
    TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT
    ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG
    GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA
    CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC
    GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA
    CGACTGCTCT ACGCCGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260491.1
    CDS175..1455
    Translation

    Target ORF information:

    RefSeq Version NM_001273562.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twine (twe), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273562.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
    GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA
    TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC
    ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC
    ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG
    TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG
    CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG
    TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG
    GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG
    CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA
    CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG
    TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT
    CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT
    GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC
    CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG
    TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC
    TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT
    ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG
    GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA
    CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC
    GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA
    CGACTGCTCT ACGCCGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa14732
    Clone ID Related Accession (Same CDS sequence) NM_057285.4 , NM_001273564.2 , NM_001273562.2 , NM_001273563.1
    Accession Version NM_001273563.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twine, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
    GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA
    TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC
    ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC
    ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG
    TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG
    CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG
    TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG
    GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG
    CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA
    CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG
    TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT
    CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT
    GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC
    CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG
    TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC
    TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT
    ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG
    GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA
    CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC
    GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA
    CGACTGCTCT ACGCCGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260492.1
    CDS149..1429
    Translation

    Target ORF information:

    RefSeq Version NM_001273563.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twine (twe), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273563.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGAGCA AGCGGCTAAT GCTCGATGTG GAGGAAGAAG ATGATGAAAG TGGAGCGTGT 
    GGTCAGGAGA ATTTCGATCC CCATGATGCC GATATGGAAT ACCAGGCCAA AAGGCGAAAA
    TCAGCCGTGC AGGAGACTCC GCTGCAATGG ATGCTGAAGC GGCATATTCC TGCCAGCACC
    ACCGTTCTGT CGCCCATCAC CGAATTGTCG CAGAATATGA ATGGAGCCCG CCTGGATGGC
    ACTCCCAAGT CCACCCAGAG AATCCCAGCC AACAGGACCC TGAATAACTT TAATAGCCTG
    TCCTCGCGAA CACTCGGCAG TTTCAGTAGC TCCTGCTCGA GTTACGAGTC GGGCAACTCG
    CTGGATGATG AGTACATGGA CATGTTCGAA ATGGAATCAG CCGAGAATCA CAATCTTGAG
    TTGCCCGATG ACTTGGAAGT GCTCCTCAGC GGACAGCTGA AATCGGAAAG CAATTTAGAG
    GAGATGTCCA ACAAAAAAGG CTCCCTGCGT CGCTGCCTTA GCATGTATCC CAGTGAACAG
    CCAGAGGAAG CGGTTCAAGA ACCAGATCAG GAGACCAACA TGCCCATGAA GAAGATGCAA
    CGGAAAACTC TATCAATGAA CGACGCCGAG ATCATGAGAG CACTGGGAGA TGAACCAGAG
    TTAATCGGAG ATCTCAGCAA GCCATGTACC CTGCCCTGTT TGGCGACAGG CATTAGACAT
    CGTGATCTAA AGACCATCTC CAGTGACACA TTGGCCAGAC TGATTCAGGG CGAGTTTGAT
    GAACAATTGG GAAGCCAGGG CGGATACGAG ATCATAGACT GCCGTTACCC ATACGAGTTC
    CTTGGCGGAC ACATACGGGG AGCGAAGAAT TTGTACACAC GTGGGCAGAT ACAGGAGGCG
    TTTCCCACGC TGACCTCCAA TCAGGAAAAT CGACGCATCT ACGTCTTCCA CTGTGAGTTC
    TCCTCGGAAC GGGGTCCCAA GCTATTGCGC TACCTGCGGA GCAACGACAG AAGTCAGCAT
    ACCCACAACT ATCCGGCACT GGACTATCCT GAACTTTATA TCCTGCACAA TGGGTACAAG
    GAGTTCTTCG GTCTATACTC GCAGCTATGC CAGCCCAGCC AATATGTACC AATGCTGGCA
    CCGGCGCACA ATGATGAGTT TCGATATTTT CGGGCCAAGA CCAAGTCCTG GCAATGTGGC
    GAGGGCGGAG ACAGTGGAAT CGGCGGTGGA GGCTCTCGCG GCCTCCGGAA ATCACGTTCA
    CGACTGCTCT ACGCCGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.