Gli cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gli
Entrez Gene ID	34927
Full Name	Gliotactin
Synonyms	BG:DS09217.3,CG3903,Dmel\CG3903,gli,l(2)35Dg,l(2)br45,n(2)k09033
General protein information	Preferred Names Gliotactin Names CG3903 gene product from transcript CG3903-RD CG3903-PA CG3903-PC CG3903-PD CG3903-PE CG3903-PF Gli-PA Gli-PC Gli-PD Gli-PE Gli-PF gliotactin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35D4-35D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_165128.2	NP_723929.1	gliotactin, isoform D
NM_057254.4	NP_476602.1	gliotactin, isoform A
NM_165127.2	NP_723928.1	gliotactin, isoform C
NM_165130.2	NP_723931.2	gliotactin, isoform F
NM_165129.2	NP_723930.1	gliotactin, isoform E

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Gli	NP_476602.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008190	XP_317277.4
Caenorhabditis elegans (roundworm)	glit-1	NP_508807.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gliotactin leads to a feedback loop to control Gliotactin mRNA levels thorough Tkv protein signalling and induction of miR-184.
Title: The Drosophila tricellular junction protein Gliotactin regulates its own mRNA levels through BMP-mediated induction of miR-184.

A Comparison of Ci/Gli Activity as Regulated by Sufu in Drosophila and Mammalian Hedgehog Response
Title: A Comparison of Ci/Gli Activity as Regulated by Sufu in Drosophila and Mammalian Hedgehog Response.

Gliotactin and Discs large are co-regulated to maintain epithelial integrity.
Title: Gliotactin and Discs large are co-regulated to maintain epithelial integrity.

Gliotactin levels within the cell membrane are regulated to ensure correct protein localization and cell survival.
Title: Control of Gliotactin localization and levels by tyrosine phosphorylation and endocytosis is necessary for survival of polarized epithelia.

These results suggest a target gene-selective involvement of the PAF1 complex in Hh signaling via the Parafibromin/Hyx-mediated recruitment to Gli/Ci.
Title: The role of Parafibromin/Hyrax as a nuclear Gli/Ci-interacting protein in Hedgehog target gene control.

The following Gli gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gli cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16796	NM_057254.4 Latest version!	Drosophila melanogaster gliotactin, transcript variant A (Gli), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16796	NM_165129.2 Latest version!	Drosophila melanogaster gliotactin, transcript variant E (Gli), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16796	NM_165130.2 Latest version!	Drosophila melanogaster gliotactin, transcript variant F (Gli), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16796	NM_165128.2 Latest version!	Drosophila melanogaster gliotactin, transcript variant D (Gli), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16796	NM_165127.2 Latest version!	Drosophila melanogaster gliotactin, transcript variant C (Gli), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gli ( NM_165128.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723929.1 Drosophila melanogaster gliotactin, transcript variant D (Gli), mRNA.

Price & Availability↑

CloneID	OFa16796
Clone ID Related Accession (Same CDS sequence)	NM_165128.2 , NM_057254.4 , NM_165127.2 , NM_165130.2 , NM_165129.2
Accession Version	NM_165128.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gliotactin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165128.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCACA AATTGAAATA TCGCGATAAA TTAAAATGGC TTTTAGCCCT TCTTGTGCTG 
ATCGGCACTT GTTTTATTCA GACAAGGGGA CAAACAAGAG ATCCCAGATT TTATTCTCGG 
CCAGGCGTTG ACTACCATTG GCCAAATCCA GGCGATCCGG ATTACAGAAC CTACACGTTC 
AACGATCGCC GATATGGTCA TTATCAGCCA AATGGCTATG GAGCCAACTA TCCAGGCAGA 
AATCCACCGG GACAATATCC ACAGGGAATG CCGAATGAAG ATCGCTTTCG ATTTGACCCG 
AACGATCCGA ATGCGAGAAC CCAGTTTCCG GGAGTGCTGG CCGGATGGCG AGAGGATTTG 
CAGGGCAAGC AGCGGCGGGA TTCGTTGACC CTGGAGCGGG ATGTTTTCGT GACCACCAAC 
TATGGCCAGG TGCAGGGCTT TAAGGTGTAC ATGTACGATA ATCCAGATCC GAAGTCCTTC 
TATCGTCCCT ACCACTCGAC CGTGGATCGT GTGATGGGCG AGTGCTCGGT CTTCCTGGGC 
ATTCCCTACG CCCTGCCGCC CACCTTCGAG GGCAGGTTCA AGCCACCACG CGTCCATCGA 
GGCTGGCAGC TGCTGCAGGC CGTCGACTTT GGACCCGCCT GTCCACAGCC TGTGCGATAT 
ACGGGTGCCA CGAAAGGAAT CATGGACATG GACGAGGATT GCCTCTACTT GAACGTGTAT 
TCGCCGAAGA CTGGTGCTGG TGTGGCTCAA AAATACCCGG TTATGGTGTA CATCCATGGC 
GGCGAGTTCA TTCGTGGAGC CTCCAACCTA TTCCAGGGTC ATATTCTGGC CTCGTTCTAC 
GACGTGGTCG TGGTGACCCT GAATTACCGC CTTGGTGCCC TGGGATTCCT ATCGACGGGT 
GATGAGAACT CGCCCGGAAA CTACGGAATC CTCGATCAAG CGATGGCGCT ACGTTGGGTC 
TATGACAATA TTGAGTTCTT CAACGGCGAT CGGAATTCCA TCACTCTATT TGGTCCGGGA 
GCAGGAGGCG CCTCCGCTGG ACTCCTGATG GTGGCACCAC AGACGCGGAA CATTGTGCGT 
CGTGTGATCG CACAGTCCGG ATCGGCTCTA GCGGATTGGG CGCTCATCCA GGACAAGTAT 
CGCGCCCAGA ACACGAGTCG CGTGCTGGGA CAGCTGCTGG GCTGCTCCAT TGAATCGTCG 
TGGAAGTTGG TCAACTGCCT GCGCACCGGA CGCAGCTTCT ATGAGCTGGG AAACGCTGAG 
TTCTCTCCCC AGGTGGGCAG CTTTCCATGG GGTCCAGTTC TGGACCACAA CTTTACGTTG 
CCCGGCGACG ATTGGTACGA GGGATGGCGC GAAAAGGATT GGCGTTTCCT CACCCAAACG 
CCGGAAACCC TCATCCGTGC CGGTAAATTC AACCGGAATA TTCAGTACAT GACGGGCGTG 
ACCACACAGG AAGCGGCCTT TTTTGTGGCC CAAAACGAAT CCCTAAGTCC GTACTATGAA 
CTAGATGGAC GTTTCTTCGA TCAGAAAATA AGGGAACACG TTTTCCGCTA CAACTATACA 
CTTAATCCGA ACGGAGTTTA CGAGGCCATC AAGTACATAT ACACCTTCTG GCCGGATCCC 
AATAATAACA CCATAATCCG GGACCAGTAC ATAAACATGC TGAGTGATCT CTACTACCGA 
GCACCGGTGG ATCAAATGGT CAAGCTAATG CTGGAGCAGA AGGTACCCGT TTATATGTAC 
GTACTGAACA CCACTGTGGA GGCACTGAAT CTGCCACAGT GGCGAAAGTA TCCACACGAC 
ATCGAACGTT ATTTCCTCAC CGGAGCTCCC TTCATGGACA CCGAGTTCTT TCCCAAAAAG 
GAGCATCTGC AGCGCAATAT GTGGACGGAT AACGATCGCA ATATGAGTCA CTTTTTCATG 
CAGACCTACA CGAATTTTGC TAGATATGGC AATCCGACGC CGCAACAGGT GCTAGGCATG 
CATTTCCAGC GCGCATACCA GGGCGAGATT CGGTACTTGA ACATCAATAC CACGTACAAC 
TCCTCCATTC TACTCAACTA TCGGCAGACG GAGTGCGCCT TCTGGACGCA ATACTTGCCC 
ACAGTTATTG GAGTGCTGGT GCCCACTTAT CCACCCACCA CGGAGTATTG GTGGGAGCCC 
AAGGAGCCAC TACAGATCGC CTTTTGGAGC ATGTCGGTGG CCTGTTTCTT CCTCATAGTC 
CTGGTGGTCA TCTGCTGCAT CATGTGGCGC AATGCCAAGC GCCAATCGGA TCGCTTCTAT 
GACGAAGATG TCTTCATTAA TGGTGAGGGC TTGGAACCGG AACAGGATAC GCGTGGAGTG 
GACAATGCCC ACATGGTGAC CAACCATCAT GCCCTGCGCT CCAGGGATAA TATCTACGAG 
TACCGCGACT CTCCATCCAC CAAAACCTTG GCCAGCAAAG CGCACACGGA CACCACCTCG 
TTGCGCTCAC CCAGTTCGCT GGCCATGACC CAAAAGTCCA GCAGCCAGGC GTCCCTCAAG 
TCAGGGATCT CGCTCAAGGA AACCAATGGC CATTTGGTGA AGCAATCTGA AAGGGCAGCC 
ACGCCACGAT CCCAACAAAA TGGGTCCACC GCAAAGGTGG CGTCTCCTCC TGTGGAGGAG 
AAGCGTCTAC TGCAGCCACT TTCCAGCACG CCCGTGACGC AGTTGCAGGC GGAGCCGGCC 
AAAAGAGTTC CCACCGCTGC CAGTGTCTCG GGCAGCAGTC GGAGCACCAC TCCGGTGCCC 
TCTGCCCGCA GCACCACCAC GCACACCACA ACAGCCACCC TGAGTTCCCA GCCAGCGGCT 
CAGCCGAGGA GAACCCACCT GGTGGAGGGA GTGCCTCAGA CATCCGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723929.1
CDS	77..2947
Translation	MMHKLKYRDKLKWLLALLVLIGTCFIQTRGQTRDPRFYSRPGVDYHWPNPGDPDYRTYTFNDRRYGHYQPNGYGANYPGRNPPGQYPQGMPNEDRFRFDPNDPNARTQFPGVLAGWREDLQGKQRRDSLTLERDVFVTTNYGQVQGFKVYMYDNPDPKSFYRPYHSTVDRVMGECSVFLGIPYALPPTFEGRFKPPRVHRGWQLLQAVDFGPACPQPVRYTGATKGIMDMDEDCLYLNVYSPKTGAGVAQKYPVMVYIHGGEFIRGASNLFQGHILASFYDVVVVTLNYRLGALGFLSTGDENSPGNYGILDQAMALRWVYDNIEFFNGDRNSITLFGPGAGGASAGLLMVAPQTRNIVRRVIAQSGSALADWALIQDKYRAQNTSRVLGQLLGCSIESSWKLVNCLRTGRSFYELGNAEFSPQVGSFPWGPVLDHNFTLPGDDWYEGWREKDWRFLTQTPETLIRAGKFNRNIQYMTGVTTQEAAFFVAQNESLSPYYELDGRFFDQKIREHVFRYNYTLNPNGVYEAIKYIYTFWPDPNNNTIIRDQYINMLSDLYYRAPVDQMVKLMLEQKVPVYMYVLNTTVEALNLPQWRKYPHDIERYFLTGAPFMDTEFFPKKEHLQRNMWTDNDRNMSHFFMQTYTNFARYGNPTPQQVLGMHFQRAYQGEIRYLNINTTYNSSILLNYRQTECAFWTQYLPTVIGVLVPTYPPTTEYWWEPKEPLQIAFWSMSVACFFLIVLVVICCIMWRNAKRQSDRFYDEDVFINGEGLEPEQDTRGVDNAHMVTNHHALRSRDNIYEYRDSPSTKTLASKAHTDTTSLRSPSSLAMTQKSSSQASLKSGISLKETNGHLVKQSERAATPRSQQNGSTAKVASPPVEEKRLLQPLSSTPVTQLQAEPAKRVPTAASVSGSSRSTTPVPSARSTTTHTTTATLSSQPAAQPRRTHLVEGVPQTSV

Target ORF information:

RefSeq Version	NM_165128.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gliotactin, transcript variant D (Gli), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165128.2

ORF Insert Sequence:

ATGATGCACA AATTGAAATA TCGCGATAAA TTAAAATGGC TTTTAGCCCT TCTTGTGCTG 
ATCGGCACTT GTTTTATTCA GACAAGGGGA CAAACAAGAG ATCCCAGATT TTATTCTCGG 
CCAGGCGTTG ACTACCATTG GCCAAATCCA GGCGATCCGG ATTACAGAAC CTACACGTTC 
AACGATCGCC GATATGGTCA TTATCAGCCA AATGGCTATG GAGCCAACTA TCCAGGCAGA 
AATCCACCGG GACAATATCC ACAGGGAATG CCGAATGAAG ATCGCTTTCG ATTTGACCCG 
AACGATCCGA ATGCGAGAAC CCAGTTTCCG GGAGTGCTGG CCGGATGGCG AGAGGATTTG 
CAGGGCAAGC AGCGGCGGGA TTCGTTGACC CTGGAGCGGG ATGTTTTCGT GACCACCAAC 
TATGGCCAGG TGCAGGGCTT TAAGGTGTAC ATGTACGATA ATCCAGATCC GAAGTCCTTC 
TATCGTCCCT ACCACTCGAC CGTGGATCGT GTGATGGGCG AGTGCTCGGT CTTCCTGGGC 
ATTCCCTACG CCCTGCCGCC CACCTTCGAG GGCAGGTTCA AGCCACCACG CGTCCATCGA 
GGCTGGCAGC TGCTGCAGGC CGTCGACTTT GGACCCGCCT GTCCACAGCC TGTGCGATAT 
ACGGGTGCCA CGAAAGGAAT CATGGACATG GACGAGGATT GCCTCTACTT GAACGTGTAT 
TCGCCGAAGA CTGGTGCTGG TGTGGCTCAA AAATACCCGG TTATGGTGTA CATCCATGGC 
GGCGAGTTCA TTCGTGGAGC CTCCAACCTA TTCCAGGGTC ATATTCTGGC CTCGTTCTAC 
GACGTGGTCG TGGTGACCCT GAATTACCGC CTTGGTGCCC TGGGATTCCT ATCGACGGGT 
GATGAGAACT CGCCCGGAAA CTACGGAATC CTCGATCAAG CGATGGCGCT ACGTTGGGTC 
TATGACAATA TTGAGTTCTT CAACGGCGAT CGGAATTCCA TCACTCTATT TGGTCCGGGA 
GCAGGAGGCG CCTCCGCTGG ACTCCTGATG GTGGCACCAC AGACGCGGAA CATTGTGCGT 
CGTGTGATCG CACAGTCCGG ATCGGCTCTA GCGGATTGGG CGCTCATCCA GGACAAGTAT 
CGCGCCCAGA ACACGAGTCG CGTGCTGGGA CAGCTGCTGG GCTGCTCCAT TGAATCGTCG 
TGGAAGTTGG TCAACTGCCT GCGCACCGGA CGCAGCTTCT ATGAGCTGGG AAACGCTGAG 
TTCTCTCCCC AGGTGGGCAG CTTTCCATGG GGTCCAGTTC TGGACCACAA CTTTACGTTG 
CCCGGCGACG ATTGGTACGA GGGATGGCGC GAAAAGGATT GGCGTTTCCT CACCCAAACG 
CCGGAAACCC TCATCCGTGC CGGTAAATTC AACCGGAATA TTCAGTACAT GACGGGCGTG 
ACCACACAGG AAGCGGCCTT TTTTGTGGCC CAAAACGAAT CCCTAAGTCC GTACTATGAA 
CTAGATGGAC GTTTCTTCGA TCAGAAAATA AGGGAACACG TTTTCCGCTA CAACTATACA 
CTTAATCCGA ACGGAGTTTA CGAGGCCATC AAGTACATAT ACACCTTCTG GCCGGATCCC 
AATAATAACA CCATAATCCG GGACCAGTAC ATAAACATGC TGAGTGATCT CTACTACCGA 
GCACCGGTGG ATCAAATGGT CAAGCTAATG CTGGAGCAGA AGGTACCCGT TTATATGTAC 
GTACTGAACA CCACTGTGGA GGCACTGAAT CTGCCACAGT GGCGAAAGTA TCCACACGAC 
ATCGAACGTT ATTTCCTCAC CGGAGCTCCC TTCATGGACA CCGAGTTCTT TCCCAAAAAG 
GAGCATCTGC AGCGCAATAT GTGGACGGAT AACGATCGCA ATATGAGTCA CTTTTTCATG 
CAGACCTACA CGAATTTTGC TAGATATGGC AATCCGACGC CGCAACAGGT GCTAGGCATG 
CATTTCCAGC GCGCATACCA GGGCGAGATT CGGTACTTGA ACATCAATAC CACGTACAAC 
TCCTCCATTC TACTCAACTA TCGGCAGACG GAGTGCGCCT TCTGGACGCA ATACTTGCCC 
ACAGTTATTG GAGTGCTGGT GCCCACTTAT CCACCCACCA CGGAGTATTG GTGGGAGCCC 
AAGGAGCCAC TACAGATCGC CTTTTGGAGC ATGTCGGTGG CCTGTTTCTT CCTCATAGTC 
CTGGTGGTCA TCTGCTGCAT CATGTGGCGC AATGCCAAGC GCCAATCGGA TCGCTTCTAT 
GACGAAGATG TCTTCATTAA TGGTGAGGGC TTGGAACCGG AACAGGATAC GCGTGGAGTG 
GACAATGCCC ACATGGTGAC CAACCATCAT GCCCTGCGCT CCAGGGATAA TATCTACGAG 
TACCGCGACT CTCCATCCAC CAAAACCTTG GCCAGCAAAG CGCACACGGA CACCACCTCG 
TTGCGCTCAC CCAGTTCGCT GGCCATGACC CAAAAGTCCA GCAGCCAGGC GTCCCTCAAG 
TCAGGGATCT CGCTCAAGGA AACCAATGGC CATTTGGTGA AGCAATCTGA AAGGGCAGCC 
ACGCCACGAT CCCAACAAAA TGGGTCCACC GCAAAGGTGG CGTCTCCTCC TGTGGAGGAG 
AAGCGTCTAC TGCAGCCACT TTCCAGCACG CCCGTGACGC AGTTGCAGGC GGAGCCGGCC 
AAAAGAGTTC CCACCGCTGC CAGTGTCTCG GGCAGCAGTC GGAGCACCAC TCCGGTGCCC 
TCTGCCCGCA GCACCACCAC GCACACCACA ACAGCCACCC TGAGTTCCCA GCCAGCGGCT 
CAGCCGAGGA GAACCCACCT GGTGGAGGGA GTGCCTCAGA CATCCGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli ( NM_057254.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476602.1 Drosophila melanogaster gliotactin, transcript variant A (Gli), mRNA.

Price & Availability↑

CloneID	OFa16796
Clone ID Related Accession (Same CDS sequence)	NM_165128.2 , NM_057254.4 , NM_165127.2 , NM_165130.2 , NM_165129.2
Accession Version	NM_057254.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gliotactin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057254.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCACA AATTGAAATA TCGCGATAAA TTAAAATGGC TTTTAGCCCT TCTTGTGCTG 
ATCGGCACTT GTTTTATTCA GACAAGGGGA CAAACAAGAG ATCCCAGATT TTATTCTCGG 
CCAGGCGTTG ACTACCATTG GCCAAATCCA GGCGATCCGG ATTACAGAAC CTACACGTTC 
AACGATCGCC GATATGGTCA TTATCAGCCA AATGGCTATG GAGCCAACTA TCCAGGCAGA 
AATCCACCGG GACAATATCC ACAGGGAATG CCGAATGAAG ATCGCTTTCG ATTTGACCCG 
AACGATCCGA ATGCGAGAAC CCAGTTTCCG GGAGTGCTGG CCGGATGGCG AGAGGATTTG 
CAGGGCAAGC AGCGGCGGGA TTCGTTGACC CTGGAGCGGG ATGTTTTCGT GACCACCAAC 
TATGGCCAGG TGCAGGGCTT TAAGGTGTAC ATGTACGATA ATCCAGATCC GAAGTCCTTC 
TATCGTCCCT ACCACTCGAC CGTGGATCGT GTGATGGGCG AGTGCTCGGT CTTCCTGGGC 
ATTCCCTACG CCCTGCCGCC CACCTTCGAG GGCAGGTTCA AGCCACCACG CGTCCATCGA 
GGCTGGCAGC TGCTGCAGGC CGTCGACTTT GGACCCGCCT GTCCACAGCC TGTGCGATAT 
ACGGGTGCCA CGAAAGGAAT CATGGACATG GACGAGGATT GCCTCTACTT GAACGTGTAT 
TCGCCGAAGA CTGGTGCTGG TGTGGCTCAA AAATACCCGG TTATGGTGTA CATCCATGGC 
GGCGAGTTCA TTCGTGGAGC CTCCAACCTA TTCCAGGGTC ATATTCTGGC CTCGTTCTAC 
GACGTGGTCG TGGTGACCCT GAATTACCGC CTTGGTGCCC TGGGATTCCT ATCGACGGGT 
GATGAGAACT CGCCCGGAAA CTACGGAATC CTCGATCAAG CGATGGCGCT ACGTTGGGTC 
TATGACAATA TTGAGTTCTT CAACGGCGAT CGGAATTCCA TCACTCTATT TGGTCCGGGA 
GCAGGAGGCG CCTCCGCTGG ACTCCTGATG GTGGCACCAC AGACGCGGAA CATTGTGCGT 
CGTGTGATCG CACAGTCCGG ATCGGCTCTA GCGGATTGGG CGCTCATCCA GGACAAGTAT 
CGCGCCCAGA ACACGAGTCG CGTGCTGGGA CAGCTGCTGG GCTGCTCCAT TGAATCGTCG 
TGGAAGTTGG TCAACTGCCT GCGCACCGGA CGCAGCTTCT ATGAGCTGGG AAACGCTGAG 
TTCTCTCCCC AGGTGGGCAG CTTTCCATGG GGTCCAGTTC TGGACCACAA CTTTACGTTG 
CCCGGCGACG ATTGGTACGA GGGATGGCGC GAAAAGGATT GGCGTTTCCT CACCCAAACG 
CCGGAAACCC TCATCCGTGC CGGTAAATTC AACCGGAATA TTCAGTACAT GACGGGCGTG 
ACCACACAGG AAGCGGCCTT TTTTGTGGCC CAAAACGAAT CCCTAAGTCC GTACTATGAA 
CTAGATGGAC GTTTCTTCGA TCAGAAAATA AGGGAACACG TTTTCCGCTA CAACTATACA 
CTTAATCCGA ACGGAGTTTA CGAGGCCATC AAGTACATAT ACACCTTCTG GCCGGATCCC 
AATAATAACA CCATAATCCG GGACCAGTAC ATAAACATGC TGAGTGATCT CTACTACCGA 
GCACCGGTGG ATCAAATGGT CAAGCTAATG CTGGAGCAGA AGGTACCCGT TTATATGTAC 
GTACTGAACA CCACTGTGGA GGCACTGAAT CTGCCACAGT GGCGAAAGTA TCCACACGAC 
ATCGAACGTT ATTTCCTCAC CGGAGCTCCC TTCATGGACA CCGAGTTCTT TCCCAAAAAG 
GAGCATCTGC AGCGCAATAT GTGGACGGAT AACGATCGCA ATATGAGTCA CTTTTTCATG 
CAGACCTACA CGAATTTTGC TAGATATGGC AATCCGACGC CGCAACAGGT GCTAGGCATG 
CATTTCCAGC GCGCATACCA GGGCGAGATT CGGTACTTGA ACATCAATAC CACGTACAAC 
TCCTCCATTC TACTCAACTA TCGGCAGACG GAGTGCGCCT TCTGGACGCA ATACTTGCCC 
ACAGTTATTG GAGTGCTGGT GCCCACTTAT CCACCCACCA CGGAGTATTG GTGGGAGCCC 
AAGGAGCCAC TACAGATCGC CTTTTGGAGC ATGTCGGTGG CCTGTTTCTT CCTCATAGTC 
CTGGTGGTCA TCTGCTGCAT CATGTGGCGC AATGCCAAGC GCCAATCGGA TCGCTTCTAT 
GACGAAGATG TCTTCATTAA TGGTGAGGGC TTGGAACCGG AACAGGATAC GCGTGGAGTG 
GACAATGCCC ACATGGTGAC CAACCATCAT GCCCTGCGCT CCAGGGATAA TATCTACGAG 
TACCGCGACT CTCCATCCAC CAAAACCTTG GCCAGCAAAG CGCACACGGA CACCACCTCG 
TTGCGCTCAC CCAGTTCGCT GGCCATGACC CAAAAGTCCA GCAGCCAGGC GTCCCTCAAG 
TCAGGGATCT CGCTCAAGGA AACCAATGGC CATTTGGTGA AGCAATCTGA AAGGGCAGCC 
ACGCCACGAT CCCAACAAAA TGGGTCCACC GCAAAGGTGG CGTCTCCTCC TGTGGAGGAG 
AAGCGTCTAC TGCAGCCACT TTCCAGCACG CCCGTGACGC AGTTGCAGGC GGAGCCGGCC 
AAAAGAGTTC CCACCGCTGC CAGTGTCTCG GGCAGCAGTC GGAGCACCAC TCCGGTGCCC 
TCTGCCCGCA GCACCACCAC GCACACCACA ACAGCCACCC TGAGTTCCCA GCCAGCGGCT 
CAGCCGAGGA GAACCCACCT GGTGGAGGGA GTGCCTCAGA CATCCGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476602.1
CDS	197..3067
Translation	MMHKLKYRDKLKWLLALLVLIGTCFIQTRGQTRDPRFYSRPGVDYHWPNPGDPDYRTYTFNDRRYGHYQPNGYGANYPGRNPPGQYPQGMPNEDRFRFDPNDPNARTQFPGVLAGWREDLQGKQRRDSLTLERDVFVTTNYGQVQGFKVYMYDNPDPKSFYRPYHSTVDRVMGECSVFLGIPYALPPTFEGRFKPPRVHRGWQLLQAVDFGPACPQPVRYTGATKGIMDMDEDCLYLNVYSPKTGAGVAQKYPVMVYIHGGEFIRGASNLFQGHILASFYDVVVVTLNYRLGALGFLSTGDENSPGNYGILDQAMALRWVYDNIEFFNGDRNSITLFGPGAGGASAGLLMVAPQTRNIVRRVIAQSGSALADWALIQDKYRAQNTSRVLGQLLGCSIESSWKLVNCLRTGRSFYELGNAEFSPQVGSFPWGPVLDHNFTLPGDDWYEGWREKDWRFLTQTPETLIRAGKFNRNIQYMTGVTTQEAAFFVAQNESLSPYYELDGRFFDQKIREHVFRYNYTLNPNGVYEAIKYIYTFWPDPNNNTIIRDQYINMLSDLYYRAPVDQMVKLMLEQKVPVYMYVLNTTVEALNLPQWRKYPHDIERYFLTGAPFMDTEFFPKKEHLQRNMWTDNDRNMSHFFMQTYTNFARYGNPTPQQVLGMHFQRAYQGEIRYLNINTTYNSSILLNYRQTECAFWTQYLPTVIGVLVPTYPPTTEYWWEPKEPLQIAFWSMSVACFFLIVLVVICCIMWRNAKRQSDRFYDEDVFINGEGLEPEQDTRGVDNAHMVTNHHALRSRDNIYEYRDSPSTKTLASKAHTDTTSLRSPSSLAMTQKSSSQASLKSGISLKETNGHLVKQSERAATPRSQQNGSTAKVASPPVEEKRLLQPLSSTPVTQLQAEPAKRVPTAASVSGSSRSTTPVPSARSTTTHTTTATLSSQPAAQPRRTHLVEGVPQTSV

Target ORF information:

RefSeq Version	NM_057254.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gliotactin, transcript variant A (Gli), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057254.4

ORF Insert Sequence:

ATGATGCACA AATTGAAATA TCGCGATAAA TTAAAATGGC TTTTAGCCCT TCTTGTGCTG 
ATCGGCACTT GTTTTATTCA GACAAGGGGA CAAACAAGAG ATCCCAGATT TTATTCTCGG 
CCAGGCGTTG ACTACCATTG GCCAAATCCA GGCGATCCGG ATTACAGAAC CTACACGTTC 
AACGATCGCC GATATGGTCA TTATCAGCCA AATGGCTATG GAGCCAACTA TCCAGGCAGA 
AATCCACCGG GACAATATCC ACAGGGAATG CCGAATGAAG ATCGCTTTCG ATTTGACCCG 
AACGATCCGA ATGCGAGAAC CCAGTTTCCG GGAGTGCTGG CCGGATGGCG AGAGGATTTG 
CAGGGCAAGC AGCGGCGGGA TTCGTTGACC CTGGAGCGGG ATGTTTTCGT GACCACCAAC 
TATGGCCAGG TGCAGGGCTT TAAGGTGTAC ATGTACGATA ATCCAGATCC GAAGTCCTTC 
TATCGTCCCT ACCACTCGAC CGTGGATCGT GTGATGGGCG AGTGCTCGGT CTTCCTGGGC 
ATTCCCTACG CCCTGCCGCC CACCTTCGAG GGCAGGTTCA AGCCACCACG CGTCCATCGA 
GGCTGGCAGC TGCTGCAGGC CGTCGACTTT GGACCCGCCT GTCCACAGCC TGTGCGATAT 
ACGGGTGCCA CGAAAGGAAT CATGGACATG GACGAGGATT GCCTCTACTT GAACGTGTAT 
TCGCCGAAGA CTGGTGCTGG TGTGGCTCAA AAATACCCGG TTATGGTGTA CATCCATGGC 
GGCGAGTTCA TTCGTGGAGC CTCCAACCTA TTCCAGGGTC ATATTCTGGC CTCGTTCTAC 
GACGTGGTCG TGGTGACCCT GAATTACCGC CTTGGTGCCC TGGGATTCCT ATCGACGGGT 
GATGAGAACT CGCCCGGAAA CTACGGAATC CTCGATCAAG CGATGGCGCT ACGTTGGGTC 
TATGACAATA TTGAGTTCTT CAACGGCGAT CGGAATTCCA TCACTCTATT TGGTCCGGGA 
GCAGGAGGCG CCTCCGCTGG ACTCCTGATG GTGGCACCAC AGACGCGGAA CATTGTGCGT 
CGTGTGATCG CACAGTCCGG ATCGGCTCTA GCGGATTGGG CGCTCATCCA GGACAAGTAT 
CGCGCCCAGA ACACGAGTCG CGTGCTGGGA CAGCTGCTGG GCTGCTCCAT TGAATCGTCG 
TGGAAGTTGG TCAACTGCCT GCGCACCGGA CGCAGCTTCT ATGAGCTGGG AAACGCTGAG 
TTCTCTCCCC AGGTGGGCAG CTTTCCATGG GGTCCAGTTC TGGACCACAA CTTTACGTTG 
CCCGGCGACG ATTGGTACGA GGGATGGCGC GAAAAGGATT GGCGTTTCCT CACCCAAACG 
CCGGAAACCC TCATCCGTGC CGGTAAATTC AACCGGAATA TTCAGTACAT GACGGGCGTG 
ACCACACAGG AAGCGGCCTT TTTTGTGGCC CAAAACGAAT CCCTAAGTCC GTACTATGAA 
CTAGATGGAC GTTTCTTCGA TCAGAAAATA AGGGAACACG TTTTCCGCTA CAACTATACA 
CTTAATCCGA ACGGAGTTTA CGAGGCCATC AAGTACATAT ACACCTTCTG GCCGGATCCC 
AATAATAACA CCATAATCCG GGACCAGTAC ATAAACATGC TGAGTGATCT CTACTACCGA 
GCACCGGTGG ATCAAATGGT CAAGCTAATG CTGGAGCAGA AGGTACCCGT TTATATGTAC 
GTACTGAACA CCACTGTGGA GGCACTGAAT CTGCCACAGT GGCGAAAGTA TCCACACGAC 
ATCGAACGTT ATTTCCTCAC CGGAGCTCCC TTCATGGACA CCGAGTTCTT TCCCAAAAAG 
GAGCATCTGC AGCGCAATAT GTGGACGGAT AACGATCGCA ATATGAGTCA CTTTTTCATG 
CAGACCTACA CGAATTTTGC TAGATATGGC AATCCGACGC CGCAACAGGT GCTAGGCATG 
CATTTCCAGC GCGCATACCA GGGCGAGATT CGGTACTTGA ACATCAATAC CACGTACAAC 
TCCTCCATTC TACTCAACTA TCGGCAGACG GAGTGCGCCT TCTGGACGCA ATACTTGCCC 
ACAGTTATTG GAGTGCTGGT GCCCACTTAT CCACCCACCA CGGAGTATTG GTGGGAGCCC 
AAGGAGCCAC TACAGATCGC CTTTTGGAGC ATGTCGGTGG CCTGTTTCTT CCTCATAGTC 
CTGGTGGTCA TCTGCTGCAT CATGTGGCGC AATGCCAAGC GCCAATCGGA TCGCTTCTAT 
GACGAAGATG TCTTCATTAA TGGTGAGGGC TTGGAACCGG AACAGGATAC GCGTGGAGTG 
GACAATGCCC ACATGGTGAC CAACCATCAT GCCCTGCGCT CCAGGGATAA TATCTACGAG 
TACCGCGACT CTCCATCCAC CAAAACCTTG GCCAGCAAAG CGCACACGGA CACCACCTCG 
TTGCGCTCAC CCAGTTCGCT GGCCATGACC CAAAAGTCCA GCAGCCAGGC GTCCCTCAAG 
TCAGGGATCT CGCTCAAGGA AACCAATGGC CATTTGGTGA AGCAATCTGA AAGGGCAGCC 
ACGCCACGAT CCCAACAAAA TGGGTCCACC GCAAAGGTGG CGTCTCCTCC TGTGGAGGAG 
AAGCGTCTAC TGCAGCCACT TTCCAGCACG CCCGTGACGC AGTTGCAGGC GGAGCCGGCC 
AAAAGAGTTC CCACCGCTGC CAGTGTCTCG GGCAGCAGTC GGAGCACCAC TCCGGTGCCC 
TCTGCCCGCA GCACCACCAC GCACACCACA ACAGCCACCC TGAGTTCCCA GCCAGCGGCT 
CAGCCGAGGA GAACCCACCT GGTGGAGGGA GTGCCTCAGA CATCCGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli ( NM_165127.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723928.1 Drosophila melanogaster gliotactin, transcript variant C (Gli), mRNA.

Price & Availability↑

CloneID	OFa16796
Clone ID Related Accession (Same CDS sequence)	NM_165128.2 , NM_057254.4 , NM_165127.2 , NM_165130.2 , NM_165129.2
Accession Version	NM_165127.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gliotactin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165127.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCACA AATTGAAATA TCGCGATAAA TTAAAATGGC TTTTAGCCCT TCTTGTGCTG 
ATCGGCACTT GTTTTATTCA GACAAGGGGA CAAACAAGAG ATCCCAGATT TTATTCTCGG 
CCAGGCGTTG ACTACCATTG GCCAAATCCA GGCGATCCGG ATTACAGAAC CTACACGTTC 
AACGATCGCC GATATGGTCA TTATCAGCCA AATGGCTATG GAGCCAACTA TCCAGGCAGA 
AATCCACCGG GACAATATCC ACAGGGAATG CCGAATGAAG ATCGCTTTCG ATTTGACCCG 
AACGATCCGA ATGCGAGAAC CCAGTTTCCG GGAGTGCTGG CCGGATGGCG AGAGGATTTG 
CAGGGCAAGC AGCGGCGGGA TTCGTTGACC CTGGAGCGGG ATGTTTTCGT GACCACCAAC 
TATGGCCAGG TGCAGGGCTT TAAGGTGTAC ATGTACGATA ATCCAGATCC GAAGTCCTTC 
TATCGTCCCT ACCACTCGAC CGTGGATCGT GTGATGGGCG AGTGCTCGGT CTTCCTGGGC 
ATTCCCTACG CCCTGCCGCC CACCTTCGAG GGCAGGTTCA AGCCACCACG CGTCCATCGA 
GGCTGGCAGC TGCTGCAGGC CGTCGACTTT GGACCCGCCT GTCCACAGCC TGTGCGATAT 
ACGGGTGCCA CGAAAGGAAT CATGGACATG GACGAGGATT GCCTCTACTT GAACGTGTAT 
TCGCCGAAGA CTGGTGCTGG TGTGGCTCAA AAATACCCGG TTATGGTGTA CATCCATGGC 
GGCGAGTTCA TTCGTGGAGC CTCCAACCTA TTCCAGGGTC ATATTCTGGC CTCGTTCTAC 
GACGTGGTCG TGGTGACCCT GAATTACCGC CTTGGTGCCC TGGGATTCCT ATCGACGGGT 
GATGAGAACT CGCCCGGAAA CTACGGAATC CTCGATCAAG CGATGGCGCT ACGTTGGGTC 
TATGACAATA TTGAGTTCTT CAACGGCGAT CGGAATTCCA TCACTCTATT TGGTCCGGGA 
GCAGGAGGCG CCTCCGCTGG ACTCCTGATG GTGGCACCAC AGACGCGGAA CATTGTGCGT 
CGTGTGATCG CACAGTCCGG ATCGGCTCTA GCGGATTGGG CGCTCATCCA GGACAAGTAT 
CGCGCCCAGA ACACGAGTCG CGTGCTGGGA CAGCTGCTGG GCTGCTCCAT TGAATCGTCG 
TGGAAGTTGG TCAACTGCCT GCGCACCGGA CGCAGCTTCT ATGAGCTGGG AAACGCTGAG 
TTCTCTCCCC AGGTGGGCAG CTTTCCATGG GGTCCAGTTC TGGACCACAA CTTTACGTTG 
CCCGGCGACG ATTGGTACGA GGGATGGCGC GAAAAGGATT GGCGTTTCCT CACCCAAACG 
CCGGAAACCC TCATCCGTGC CGGTAAATTC AACCGGAATA TTCAGTACAT GACGGGCGTG 
ACCACACAGG AAGCGGCCTT TTTTGTGGCC CAAAACGAAT CCCTAAGTCC GTACTATGAA 
CTAGATGGAC GTTTCTTCGA TCAGAAAATA AGGGAACACG TTTTCCGCTA CAACTATACA 
CTTAATCCGA ACGGAGTTTA CGAGGCCATC AAGTACATAT ACACCTTCTG GCCGGATCCC 
AATAATAACA CCATAATCCG GGACCAGTAC ATAAACATGC TGAGTGATCT CTACTACCGA 
GCACCGGTGG ATCAAATGGT CAAGCTAATG CTGGAGCAGA AGGTACCCGT TTATATGTAC 
GTACTGAACA CCACTGTGGA GGCACTGAAT CTGCCACAGT GGCGAAAGTA TCCACACGAC 
ATCGAACGTT ATTTCCTCAC CGGAGCTCCC TTCATGGACA CCGAGTTCTT TCCCAAAAAG 
GAGCATCTGC AGCGCAATAT GTGGACGGAT AACGATCGCA ATATGAGTCA CTTTTTCATG 
CAGACCTACA CGAATTTTGC TAGATATGGC AATCCGACGC CGCAACAGGT GCTAGGCATG 
CATTTCCAGC GCGCATACCA GGGCGAGATT CGGTACTTGA ACATCAATAC CACGTACAAC 
TCCTCCATTC TACTCAACTA TCGGCAGACG GAGTGCGCCT TCTGGACGCA ATACTTGCCC 
ACAGTTATTG GAGTGCTGGT GCCCACTTAT CCACCCACCA CGGAGTATTG GTGGGAGCCC 
AAGGAGCCAC TACAGATCGC CTTTTGGAGC ATGTCGGTGG CCTGTTTCTT CCTCATAGTC 
CTGGTGGTCA TCTGCTGCAT CATGTGGCGC AATGCCAAGC GCCAATCGGA TCGCTTCTAT 
GACGAAGATG TCTTCATTAA TGGTGAGGGC TTGGAACCGG AACAGGATAC GCGTGGAGTG 
GACAATGCCC ACATGGTGAC CAACCATCAT GCCCTGCGCT CCAGGGATAA TATCTACGAG 
TACCGCGACT CTCCATCCAC CAAAACCTTG GCCAGCAAAG CGCACACGGA CACCACCTCG 
TTGCGCTCAC CCAGTTCGCT GGCCATGACC CAAAAGTCCA GCAGCCAGGC GTCCCTCAAG 
TCAGGGATCT CGCTCAAGGA AACCAATGGC CATTTGGTGA AGCAATCTGA AAGGGCAGCC 
ACGCCACGAT CCCAACAAAA TGGGTCCACC GCAAAGGTGG CGTCTCCTCC TGTGGAGGAG 
AAGCGTCTAC TGCAGCCACT TTCCAGCACG CCCGTGACGC AGTTGCAGGC GGAGCCGGCC 
AAAAGAGTTC CCACCGCTGC CAGTGTCTCG GGCAGCAGTC GGAGCACCAC TCCGGTGCCC 
TCTGCCCGCA GCACCACCAC GCACACCACA ACAGCCACCC TGAGTTCCCA GCCAGCGGCT 
CAGCCGAGGA GAACCCACCT GGTGGAGGGA GTGCCTCAGA CATCCGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723928.1
CDS	288..3158
Translation	MMHKLKYRDKLKWLLALLVLIGTCFIQTRGQTRDPRFYSRPGVDYHWPNPGDPDYRTYTFNDRRYGHYQPNGYGANYPGRNPPGQYPQGMPNEDRFRFDPNDPNARTQFPGVLAGWREDLQGKQRRDSLTLERDVFVTTNYGQVQGFKVYMYDNPDPKSFYRPYHSTVDRVMGECSVFLGIPYALPPTFEGRFKPPRVHRGWQLLQAVDFGPACPQPVRYTGATKGIMDMDEDCLYLNVYSPKTGAGVAQKYPVMVYIHGGEFIRGASNLFQGHILASFYDVVVVTLNYRLGALGFLSTGDENSPGNYGILDQAMALRWVYDNIEFFNGDRNSITLFGPGAGGASAGLLMVAPQTRNIVRRVIAQSGSALADWALIQDKYRAQNTSRVLGQLLGCSIESSWKLVNCLRTGRSFYELGNAEFSPQVGSFPWGPVLDHNFTLPGDDWYEGWREKDWRFLTQTPETLIRAGKFNRNIQYMTGVTTQEAAFFVAQNESLSPYYELDGRFFDQKIREHVFRYNYTLNPNGVYEAIKYIYTFWPDPNNNTIIRDQYINMLSDLYYRAPVDQMVKLMLEQKVPVYMYVLNTTVEALNLPQWRKYPHDIERYFLTGAPFMDTEFFPKKEHLQRNMWTDNDRNMSHFFMQTYTNFARYGNPTPQQVLGMHFQRAYQGEIRYLNINTTYNSSILLNYRQTECAFWTQYLPTVIGVLVPTYPPTTEYWWEPKEPLQIAFWSMSVACFFLIVLVVICCIMWRNAKRQSDRFYDEDVFINGEGLEPEQDTRGVDNAHMVTNHHALRSRDNIYEYRDSPSTKTLASKAHTDTTSLRSPSSLAMTQKSSSQASLKSGISLKETNGHLVKQSERAATPRSQQNGSTAKVASPPVEEKRLLQPLSSTPVTQLQAEPAKRVPTAASVSGSSRSTTPVPSARSTTTHTTTATLSSQPAAQPRRTHLVEGVPQTSV

Target ORF information:

RefSeq Version	NM_165127.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gliotactin, transcript variant C (Gli), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165127.2

ORF Insert Sequence:

ATGATGCACA AATTGAAATA TCGCGATAAA TTAAAATGGC TTTTAGCCCT TCTTGTGCTG 
ATCGGCACTT GTTTTATTCA GACAAGGGGA CAAACAAGAG ATCCCAGATT TTATTCTCGG 
CCAGGCGTTG ACTACCATTG GCCAAATCCA GGCGATCCGG ATTACAGAAC CTACACGTTC 
AACGATCGCC GATATGGTCA TTATCAGCCA AATGGCTATG GAGCCAACTA TCCAGGCAGA 
AATCCACCGG GACAATATCC ACAGGGAATG CCGAATGAAG ATCGCTTTCG ATTTGACCCG 
AACGATCCGA ATGCGAGAAC CCAGTTTCCG GGAGTGCTGG CCGGATGGCG AGAGGATTTG 
CAGGGCAAGC AGCGGCGGGA TTCGTTGACC CTGGAGCGGG ATGTTTTCGT GACCACCAAC 
TATGGCCAGG TGCAGGGCTT TAAGGTGTAC ATGTACGATA ATCCAGATCC GAAGTCCTTC 
TATCGTCCCT ACCACTCGAC CGTGGATCGT GTGATGGGCG AGTGCTCGGT CTTCCTGGGC 
ATTCCCTACG CCCTGCCGCC CACCTTCGAG GGCAGGTTCA AGCCACCACG CGTCCATCGA 
GGCTGGCAGC TGCTGCAGGC CGTCGACTTT GGACCCGCCT GTCCACAGCC TGTGCGATAT 
ACGGGTGCCA CGAAAGGAAT CATGGACATG GACGAGGATT GCCTCTACTT GAACGTGTAT 
TCGCCGAAGA CTGGTGCTGG TGTGGCTCAA AAATACCCGG TTATGGTGTA CATCCATGGC 
GGCGAGTTCA TTCGTGGAGC CTCCAACCTA TTCCAGGGTC ATATTCTGGC CTCGTTCTAC 
GACGTGGTCG TGGTGACCCT GAATTACCGC CTTGGTGCCC TGGGATTCCT ATCGACGGGT 
GATGAGAACT CGCCCGGAAA CTACGGAATC CTCGATCAAG CGATGGCGCT ACGTTGGGTC 
TATGACAATA TTGAGTTCTT CAACGGCGAT CGGAATTCCA TCACTCTATT TGGTCCGGGA 
GCAGGAGGCG CCTCCGCTGG ACTCCTGATG GTGGCACCAC AGACGCGGAA CATTGTGCGT 
CGTGTGATCG CACAGTCCGG ATCGGCTCTA GCGGATTGGG CGCTCATCCA GGACAAGTAT 
CGCGCCCAGA ACACGAGTCG CGTGCTGGGA CAGCTGCTGG GCTGCTCCAT TGAATCGTCG 
TGGAAGTTGG TCAACTGCCT GCGCACCGGA CGCAGCTTCT ATGAGCTGGG AAACGCTGAG 
TTCTCTCCCC AGGTGGGCAG CTTTCCATGG GGTCCAGTTC TGGACCACAA CTTTACGTTG 
CCCGGCGACG ATTGGTACGA GGGATGGCGC GAAAAGGATT GGCGTTTCCT CACCCAAACG 
CCGGAAACCC TCATCCGTGC CGGTAAATTC AACCGGAATA TTCAGTACAT GACGGGCGTG 
ACCACACAGG AAGCGGCCTT TTTTGTGGCC CAAAACGAAT CCCTAAGTCC GTACTATGAA 
CTAGATGGAC GTTTCTTCGA TCAGAAAATA AGGGAACACG TTTTCCGCTA CAACTATACA 
CTTAATCCGA ACGGAGTTTA CGAGGCCATC AAGTACATAT ACACCTTCTG GCCGGATCCC 
AATAATAACA CCATAATCCG GGACCAGTAC ATAAACATGC TGAGTGATCT CTACTACCGA 
GCACCGGTGG ATCAAATGGT CAAGCTAATG CTGGAGCAGA AGGTACCCGT TTATATGTAC 
GTACTGAACA CCACTGTGGA GGCACTGAAT CTGCCACAGT GGCGAAAGTA TCCACACGAC 
ATCGAACGTT ATTTCCTCAC CGGAGCTCCC TTCATGGACA CCGAGTTCTT TCCCAAAAAG 
GAGCATCTGC AGCGCAATAT GTGGACGGAT AACGATCGCA ATATGAGTCA CTTTTTCATG 
CAGACCTACA CGAATTTTGC TAGATATGGC AATCCGACGC CGCAACAGGT GCTAGGCATG 
CATTTCCAGC GCGCATACCA GGGCGAGATT CGGTACTTGA ACATCAATAC CACGTACAAC 
TCCTCCATTC TACTCAACTA TCGGCAGACG GAGTGCGCCT TCTGGACGCA ATACTTGCCC 
ACAGTTATTG GAGTGCTGGT GCCCACTTAT CCACCCACCA CGGAGTATTG GTGGGAGCCC 
AAGGAGCCAC TACAGATCGC CTTTTGGAGC ATGTCGGTGG CCTGTTTCTT CCTCATAGTC 
CTGGTGGTCA TCTGCTGCAT CATGTGGCGC AATGCCAAGC GCCAATCGGA TCGCTTCTAT 
GACGAAGATG TCTTCATTAA TGGTGAGGGC TTGGAACCGG AACAGGATAC GCGTGGAGTG 
GACAATGCCC ACATGGTGAC CAACCATCAT GCCCTGCGCT CCAGGGATAA TATCTACGAG 
TACCGCGACT CTCCATCCAC CAAAACCTTG GCCAGCAAAG CGCACACGGA CACCACCTCG 
TTGCGCTCAC CCAGTTCGCT GGCCATGACC CAAAAGTCCA GCAGCCAGGC GTCCCTCAAG 
TCAGGGATCT CGCTCAAGGA AACCAATGGC CATTTGGTGA AGCAATCTGA AAGGGCAGCC 
ACGCCACGAT CCCAACAAAA TGGGTCCACC GCAAAGGTGG CGTCTCCTCC TGTGGAGGAG 
AAGCGTCTAC TGCAGCCACT TTCCAGCACG CCCGTGACGC AGTTGCAGGC GGAGCCGGCC 
AAAAGAGTTC CCACCGCTGC CAGTGTCTCG GGCAGCAGTC GGAGCACCAC TCCGGTGCCC 
TCTGCCCGCA GCACCACCAC GCACACCACA ACAGCCACCC TGAGTTCCCA GCCAGCGGCT 
CAGCCGAGGA GAACCCACCT GGTGGAGGGA GTGCCTCAGA CATCCGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli ( NM_165130.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723931.2 Drosophila melanogaster gliotactin, transcript variant F (Gli), mRNA.

Price & Availability↑

CloneID	OFa16796
Clone ID Related Accession (Same CDS sequence)	NM_165128.2 , NM_057254.4 , NM_165127.2 , NM_165130.2 , NM_165129.2
Accession Version	NM_165130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gliotactin, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165130.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCACA AATTGAAATA TCGCGATAAA TTAAAATGGC TTTTAGCCCT TCTTGTGCTG 
ATCGGCACTT GTTTTATTCA GACAAGGGGA CAAACAAGAG ATCCCAGATT TTATTCTCGG 
CCAGGCGTTG ACTACCATTG GCCAAATCCA GGCGATCCGG ATTACAGAAC CTACACGTTC 
AACGATCGCC GATATGGTCA TTATCAGCCA AATGGCTATG GAGCCAACTA TCCAGGCAGA 
AATCCACCGG GACAATATCC ACAGGGAATG CCGAATGAAG ATCGCTTTCG ATTTGACCCG 
AACGATCCGA ATGCGAGAAC CCAGTTTCCG GGAGTGCTGG CCGGATGGCG AGAGGATTTG 
CAGGGCAAGC AGCGGCGGGA TTCGTTGACC CTGGAGCGGG ATGTTTTCGT GACCACCAAC 
TATGGCCAGG TGCAGGGCTT TAAGGTGTAC ATGTACGATA ATCCAGATCC GAAGTCCTTC 
TATCGTCCCT ACCACTCGAC CGTGGATCGT GTGATGGGCG AGTGCTCGGT CTTCCTGGGC 
ATTCCCTACG CCCTGCCGCC CACCTTCGAG GGCAGGTTCA AGCCACCACG CGTCCATCGA 
GGCTGGCAGC TGCTGCAGGC CGTCGACTTT GGACCCGCCT GTCCACAGCC TGTGCGATAT 
ACGGGTGCCA CGAAAGGAAT CATGGACATG GACGAGGATT GCCTCTACTT GAACGTGTAT 
TCGCCGAAGA CTGGTGCTGG TGTGGCTCAA AAATACCCGG TTATGGTGTA CATCCATGGC 
GGCGAGTTCA TTCGTGGAGC CTCCAACCTA TTCCAGGGTC ATATTCTGGC CTCGTTCTAC 
GACGTGGTCG TGGTGACCCT GAATTACCGC CTTGGTGCCC TGGGATTCCT ATCGACGGGT 
GATGAGAACT CGCCCGGAAA CTACGGAATC CTCGATCAAG CGATGGCGCT ACGTTGGGTC 
TATGACAATA TTGAGTTCTT CAACGGCGAT CGGAATTCCA TCACTCTATT TGGTCCGGGA 
GCAGGAGGCG CCTCCGCTGG ACTCCTGATG GTGGCACCAC AGACGCGGAA CATTGTGCGT 
CGTGTGATCG CACAGTCCGG ATCGGCTCTA GCGGATTGGG CGCTCATCCA GGACAAGTAT 
CGCGCCCAGA ACACGAGTCG CGTGCTGGGA CAGCTGCTGG GCTGCTCCAT TGAATCGTCG 
TGGAAGTTGG TCAACTGCCT GCGCACCGGA CGCAGCTTCT ATGAGCTGGG AAACGCTGAG 
TTCTCTCCCC AGGTGGGCAG CTTTCCATGG GGTCCAGTTC TGGACCACAA CTTTACGTTG 
CCCGGCGACG ATTGGTACGA GGGATGGCGC GAAAAGGATT GGCGTTTCCT CACCCAAACG 
CCGGAAACCC TCATCCGTGC CGGTAAATTC AACCGGAATA TTCAGTACAT GACGGGCGTG 
ACCACACAGG AAGCGGCCTT TTTTGTGGCC CAAAACGAAT CCCTAAGTCC GTACTATGAA 
CTAGATGGAC GTTTCTTCGA TCAGAAAATA AGGGAACACG TTTTCCGCTA CAACTATACA 
CTTAATCCGA ACGGAGTTTA CGAGGCCATC AAGTACATAT ACACCTTCTG GCCGGATCCC 
AATAATAACA CCATAATCCG GGACCAGTAC ATAAACATGC TGAGTGATCT CTACTACCGA 
GCACCGGTGG ATCAAATGGT CAAGCTAATG CTGGAGCAGA AGGTACCCGT TTATATGTAC 
GTACTGAACA CCACTGTGGA GGCACTGAAT CTGCCACAGT GGCGAAAGTA TCCACACGAC 
ATCGAACGTT ATTTCCTCAC CGGAGCTCCC TTCATGGACA CCGAGTTCTT TCCCAAAAAG 
GAGCATCTGC AGCGCAATAT GTGGACGGAT AACGATCGCA ATATGAGTCA CTTTTTCATG 
CAGACCTACA CGAATTTTGC TAGATATGGC AATCCGACGC CGCAACAGGT GCTAGGCATG 
CATTTCCAGC GCGCATACCA GGGCGAGATT CGGTACTTGA ACATCAATAC CACGTACAAC 
TCCTCCATTC TACTCAACTA TCGGCAGACG GAGTGCGCCT TCTGGACGCA ATACTTGCCC 
ACAGTTATTG GAGTGCTGGT GCCCACTTAT CCACCCACCA CGGAGTATTG GTGGGAGCCC 
AAGGAGCCAC TACAGATCGC CTTTTGGAGC ATGTCGGTGG CCTGTTTCTT CCTCATAGTC 
CTGGTGGTCA TCTGCTGCAT CATGTGGCGC AATGCCAAGC GCCAATCGGA TCGCTTCTAT 
GACGAAGATG TCTTCATTAA TGGTGAGGGC TTGGAACCGG AACAGGATAC GCGTGGAGTG 
GACAATGCCC ACATGGTGAC CAACCATCAT GCCCTGCGCT CCAGGGATAA TATCTACGAG 
TACCGCGACT CTCCATCCAC CAAAACCTTG GCCAGCAAAG CGCACACGGA CACCACCTCG 
TTGCGCTCAC CCAGTTCGCT GGCCATGACC CAAAAGTCCA GCAGCCAGGC GTCCCTCAAG 
TCAGGGATCT CGCTCAAGGA AACCAATGGC CATTTGGTGA AGCAATCTGA AAGGGCAGCC 
ACGCCACGAT CCCAACAAAA TGGGTCCACC GCAAAGGTGG CGTCTCCTCC TGTGGAGGAG 
AAGCGTCTAC TGCAGCCACT TTCCAGCACG CCCGTGACGC AGTTGCAGGC GGAGCCGGCC 
AAAAGAGTTC CCACCGCTGC CAGTGTCTCG GGCAGCAGTC GGAGCACCAC TCCGGTGCCC 
TCTGCCCGCA GCACCACCAC GCACACCACA ACAGCCACCC TGAGTTCCCA GCCAGCGGCT 
CAGCCGAGGA GAACCCACCT GGTGGAGGGA GTGCCTCAGA CATCCGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723931.2
CDS	570..3440
Translation	MMHKLKYRDKLKWLLALLVLIGTCFIQTRGQTRDPRFYSRPGVDYHWPNPGDPDYRTYTFNDRRYGHYQPNGYGANYPGRNPPGQYPQGMPNEDRFRFDPNDPNARTQFPGVLAGWREDLQGKQRRDSLTLERDVFVTTNYGQVQGFKVYMYDNPDPKSFYRPYHSTVDRVMGECSVFLGIPYALPPTFEGRFKPPRVHRGWQLLQAVDFGPACPQPVRYTGATKGIMDMDEDCLYLNVYSPKTGAGVAQKYPVMVYIHGGEFIRGASNLFQGHILASFYDVVVVTLNYRLGALGFLSTGDENSPGNYGILDQAMALRWVYDNIEFFNGDRNSITLFGPGAGGASAGLLMVAPQTRNIVRRVIAQSGSALADWALIQDKYRAQNTSRVLGQLLGCSIESSWKLVNCLRTGRSFYELGNAEFSPQVGSFPWGPVLDHNFTLPGDDWYEGWREKDWRFLTQTPETLIRAGKFNRNIQYMTGVTTQEAAFFVAQNESLSPYYELDGRFFDQKIREHVFRYNYTLNPNGVYEAIKYIYTFWPDPNNNTIIRDQYINMLSDLYYRAPVDQMVKLMLEQKVPVYMYVLNTTVEALNLPQWRKYPHDIERYFLTGAPFMDTEFFPKKEHLQRNMWTDNDRNMSHFFMQTYTNFARYGNPTPQQVLGMHFQRAYQGEIRYLNINTTYNSSILLNYRQTECAFWTQYLPTVIGVLVPTYPPTTEYWWEPKEPLQIAFWSMSVACFFLIVLVVICCIMWRNAKRQSDRFYDEDVFINGEGLEPEQDTRGVDNAHMVTNHHALRSRDNIYEYRDSPSTKTLASKAHTDTTSLRSPSSLAMTQKSSSQASLKSGISLKETNGHLVKQSERAATPRSQQNGSTAKVASPPVEEKRLLQPLSSTPVTQLQAEPAKRVPTAASVSGSSRSTTPVPSARSTTTHTTTATLSSQPAAQPRRTHLVEGVPQTSV

Target ORF information:

RefSeq Version	NM_165130.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gliotactin, transcript variant F (Gli), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165130.2

ORF Insert Sequence:

ATGATGCACA AATTGAAATA TCGCGATAAA TTAAAATGGC TTTTAGCCCT TCTTGTGCTG 
ATCGGCACTT GTTTTATTCA GACAAGGGGA CAAACAAGAG ATCCCAGATT TTATTCTCGG 
CCAGGCGTTG ACTACCATTG GCCAAATCCA GGCGATCCGG ATTACAGAAC CTACACGTTC 
AACGATCGCC GATATGGTCA TTATCAGCCA AATGGCTATG GAGCCAACTA TCCAGGCAGA 
AATCCACCGG GACAATATCC ACAGGGAATG CCGAATGAAG ATCGCTTTCG ATTTGACCCG 
AACGATCCGA ATGCGAGAAC CCAGTTTCCG GGAGTGCTGG CCGGATGGCG AGAGGATTTG 
CAGGGCAAGC AGCGGCGGGA TTCGTTGACC CTGGAGCGGG ATGTTTTCGT GACCACCAAC 
TATGGCCAGG TGCAGGGCTT TAAGGTGTAC ATGTACGATA ATCCAGATCC GAAGTCCTTC 
TATCGTCCCT ACCACTCGAC CGTGGATCGT GTGATGGGCG AGTGCTCGGT CTTCCTGGGC 
ATTCCCTACG CCCTGCCGCC CACCTTCGAG GGCAGGTTCA AGCCACCACG CGTCCATCGA 
GGCTGGCAGC TGCTGCAGGC CGTCGACTTT GGACCCGCCT GTCCACAGCC TGTGCGATAT 
ACGGGTGCCA CGAAAGGAAT CATGGACATG GACGAGGATT GCCTCTACTT GAACGTGTAT 
TCGCCGAAGA CTGGTGCTGG TGTGGCTCAA AAATACCCGG TTATGGTGTA CATCCATGGC 
GGCGAGTTCA TTCGTGGAGC CTCCAACCTA TTCCAGGGTC ATATTCTGGC CTCGTTCTAC 
GACGTGGTCG TGGTGACCCT GAATTACCGC CTTGGTGCCC TGGGATTCCT ATCGACGGGT 
GATGAGAACT CGCCCGGAAA CTACGGAATC CTCGATCAAG CGATGGCGCT ACGTTGGGTC 
TATGACAATA TTGAGTTCTT CAACGGCGAT CGGAATTCCA TCACTCTATT TGGTCCGGGA 
GCAGGAGGCG CCTCCGCTGG ACTCCTGATG GTGGCACCAC AGACGCGGAA CATTGTGCGT 
CGTGTGATCG CACAGTCCGG ATCGGCTCTA GCGGATTGGG CGCTCATCCA GGACAAGTAT 
CGCGCCCAGA ACACGAGTCG CGTGCTGGGA CAGCTGCTGG GCTGCTCCAT TGAATCGTCG 
TGGAAGTTGG TCAACTGCCT GCGCACCGGA CGCAGCTTCT ATGAGCTGGG AAACGCTGAG 
TTCTCTCCCC AGGTGGGCAG CTTTCCATGG GGTCCAGTTC TGGACCACAA CTTTACGTTG 
CCCGGCGACG ATTGGTACGA GGGATGGCGC GAAAAGGATT GGCGTTTCCT CACCCAAACG 
CCGGAAACCC TCATCCGTGC CGGTAAATTC AACCGGAATA TTCAGTACAT GACGGGCGTG 
ACCACACAGG AAGCGGCCTT TTTTGTGGCC CAAAACGAAT CCCTAAGTCC GTACTATGAA 
CTAGATGGAC GTTTCTTCGA TCAGAAAATA AGGGAACACG TTTTCCGCTA CAACTATACA 
CTTAATCCGA ACGGAGTTTA CGAGGCCATC AAGTACATAT ACACCTTCTG GCCGGATCCC 
AATAATAACA CCATAATCCG GGACCAGTAC ATAAACATGC TGAGTGATCT CTACTACCGA 
GCACCGGTGG ATCAAATGGT CAAGCTAATG CTGGAGCAGA AGGTACCCGT TTATATGTAC 
GTACTGAACA CCACTGTGGA GGCACTGAAT CTGCCACAGT GGCGAAAGTA TCCACACGAC 
ATCGAACGTT ATTTCCTCAC CGGAGCTCCC TTCATGGACA CCGAGTTCTT TCCCAAAAAG 
GAGCATCTGC AGCGCAATAT GTGGACGGAT AACGATCGCA ATATGAGTCA CTTTTTCATG 
CAGACCTACA CGAATTTTGC TAGATATGGC AATCCGACGC CGCAACAGGT GCTAGGCATG 
CATTTCCAGC GCGCATACCA GGGCGAGATT CGGTACTTGA ACATCAATAC CACGTACAAC 
TCCTCCATTC TACTCAACTA TCGGCAGACG GAGTGCGCCT TCTGGACGCA ATACTTGCCC 
ACAGTTATTG GAGTGCTGGT GCCCACTTAT CCACCCACCA CGGAGTATTG GTGGGAGCCC 
AAGGAGCCAC TACAGATCGC CTTTTGGAGC ATGTCGGTGG CCTGTTTCTT CCTCATAGTC 
CTGGTGGTCA TCTGCTGCAT CATGTGGCGC AATGCCAAGC GCCAATCGGA TCGCTTCTAT 
GACGAAGATG TCTTCATTAA TGGTGAGGGC TTGGAACCGG AACAGGATAC GCGTGGAGTG 
GACAATGCCC ACATGGTGAC CAACCATCAT GCCCTGCGCT CCAGGGATAA TATCTACGAG 
TACCGCGACT CTCCATCCAC CAAAACCTTG GCCAGCAAAG CGCACACGGA CACCACCTCG 
TTGCGCTCAC CCAGTTCGCT GGCCATGACC CAAAAGTCCA GCAGCCAGGC GTCCCTCAAG 
TCAGGGATCT CGCTCAAGGA AACCAATGGC CATTTGGTGA AGCAATCTGA AAGGGCAGCC 
ACGCCACGAT CCCAACAAAA TGGGTCCACC GCAAAGGTGG CGTCTCCTCC TGTGGAGGAG 
AAGCGTCTAC TGCAGCCACT TTCCAGCACG CCCGTGACGC AGTTGCAGGC GGAGCCGGCC 
AAAAGAGTTC CCACCGCTGC CAGTGTCTCG GGCAGCAGTC GGAGCACCAC TCCGGTGCCC 
TCTGCCCGCA GCACCACCAC GCACACCACA ACAGCCACCC TGAGTTCCCA GCCAGCGGCT 
CAGCCGAGGA GAACCCACCT GGTGGAGGGA GTGCCTCAGA CATCCGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gli ( NM_165129.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723930.1 Drosophila melanogaster gliotactin, transcript variant E (Gli), mRNA.

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CloneID	OFa16796
Clone ID Related Accession (Same CDS sequence)	NM_165128.2 , NM_057254.4 , NM_165127.2 , NM_165130.2 , NM_165129.2
Accession Version	NM_165129.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gliotactin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165129.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCACA AATTGAAATA TCGCGATAAA TTAAAATGGC TTTTAGCCCT TCTTGTGCTG 
ATCGGCACTT GTTTTATTCA GACAAGGGGA CAAACAAGAG ATCCCAGATT TTATTCTCGG 
CCAGGCGTTG ACTACCATTG GCCAAATCCA GGCGATCCGG ATTACAGAAC CTACACGTTC 
AACGATCGCC GATATGGTCA TTATCAGCCA AATGGCTATG GAGCCAACTA TCCAGGCAGA 
AATCCACCGG GACAATATCC ACAGGGAATG CCGAATGAAG ATCGCTTTCG ATTTGACCCG 
AACGATCCGA ATGCGAGAAC CCAGTTTCCG GGAGTGCTGG CCGGATGGCG AGAGGATTTG 
CAGGGCAAGC AGCGGCGGGA TTCGTTGACC CTGGAGCGGG ATGTTTTCGT GACCACCAAC 
TATGGCCAGG TGCAGGGCTT TAAGGTGTAC ATGTACGATA ATCCAGATCC GAAGTCCTTC 
TATCGTCCCT ACCACTCGAC CGTGGATCGT GTGATGGGCG AGTGCTCGGT CTTCCTGGGC 
ATTCCCTACG CCCTGCCGCC CACCTTCGAG GGCAGGTTCA AGCCACCACG CGTCCATCGA 
GGCTGGCAGC TGCTGCAGGC CGTCGACTTT GGACCCGCCT GTCCACAGCC TGTGCGATAT 
ACGGGTGCCA CGAAAGGAAT CATGGACATG GACGAGGATT GCCTCTACTT GAACGTGTAT 
TCGCCGAAGA CTGGTGCTGG TGTGGCTCAA AAATACCCGG TTATGGTGTA CATCCATGGC 
GGCGAGTTCA TTCGTGGAGC CTCCAACCTA TTCCAGGGTC ATATTCTGGC CTCGTTCTAC 
GACGTGGTCG TGGTGACCCT GAATTACCGC CTTGGTGCCC TGGGATTCCT ATCGACGGGT 
GATGAGAACT CGCCCGGAAA CTACGGAATC CTCGATCAAG CGATGGCGCT ACGTTGGGTC 
TATGACAATA TTGAGTTCTT CAACGGCGAT CGGAATTCCA TCACTCTATT TGGTCCGGGA 
GCAGGAGGCG CCTCCGCTGG ACTCCTGATG GTGGCACCAC AGACGCGGAA CATTGTGCGT 
CGTGTGATCG CACAGTCCGG ATCGGCTCTA GCGGATTGGG CGCTCATCCA GGACAAGTAT 
CGCGCCCAGA ACACGAGTCG CGTGCTGGGA CAGCTGCTGG GCTGCTCCAT TGAATCGTCG 
TGGAAGTTGG TCAACTGCCT GCGCACCGGA CGCAGCTTCT ATGAGCTGGG AAACGCTGAG 
TTCTCTCCCC AGGTGGGCAG CTTTCCATGG GGTCCAGTTC TGGACCACAA CTTTACGTTG 
CCCGGCGACG ATTGGTACGA GGGATGGCGC GAAAAGGATT GGCGTTTCCT CACCCAAACG 
CCGGAAACCC TCATCCGTGC CGGTAAATTC AACCGGAATA TTCAGTACAT GACGGGCGTG 
ACCACACAGG AAGCGGCCTT TTTTGTGGCC CAAAACGAAT CCCTAAGTCC GTACTATGAA 
CTAGATGGAC GTTTCTTCGA TCAGAAAATA AGGGAACACG TTTTCCGCTA CAACTATACA 
CTTAATCCGA ACGGAGTTTA CGAGGCCATC AAGTACATAT ACACCTTCTG GCCGGATCCC 
AATAATAACA CCATAATCCG GGACCAGTAC ATAAACATGC TGAGTGATCT CTACTACCGA 
GCACCGGTGG ATCAAATGGT CAAGCTAATG CTGGAGCAGA AGGTACCCGT TTATATGTAC 
GTACTGAACA CCACTGTGGA GGCACTGAAT CTGCCACAGT GGCGAAAGTA TCCACACGAC 
ATCGAACGTT ATTTCCTCAC CGGAGCTCCC TTCATGGACA CCGAGTTCTT TCCCAAAAAG 
GAGCATCTGC AGCGCAATAT GTGGACGGAT AACGATCGCA ATATGAGTCA CTTTTTCATG 
CAGACCTACA CGAATTTTGC TAGATATGGC AATCCGACGC CGCAACAGGT GCTAGGCATG 
CATTTCCAGC GCGCATACCA GGGCGAGATT CGGTACTTGA ACATCAATAC CACGTACAAC 
TCCTCCATTC TACTCAACTA TCGGCAGACG GAGTGCGCCT TCTGGACGCA ATACTTGCCC 
ACAGTTATTG GAGTGCTGGT GCCCACTTAT CCACCCACCA CGGAGTATTG GTGGGAGCCC 
AAGGAGCCAC TACAGATCGC CTTTTGGAGC ATGTCGGTGG CCTGTTTCTT CCTCATAGTC 
CTGGTGGTCA TCTGCTGCAT CATGTGGCGC AATGCCAAGC GCCAATCGGA TCGCTTCTAT 
GACGAAGATG TCTTCATTAA TGGTGAGGGC TTGGAACCGG AACAGGATAC GCGTGGAGTG 
GACAATGCCC ACATGGTGAC CAACCATCAT GCCCTGCGCT CCAGGGATAA TATCTACGAG 
TACCGCGACT CTCCATCCAC CAAAACCTTG GCCAGCAAAG CGCACACGGA CACCACCTCG 
TTGCGCTCAC CCAGTTCGCT GGCCATGACC CAAAAGTCCA GCAGCCAGGC GTCCCTCAAG 
TCAGGGATCT CGCTCAAGGA AACCAATGGC CATTTGGTGA AGCAATCTGA AAGGGCAGCC 
ACGCCACGAT CCCAACAAAA TGGGTCCACC GCAAAGGTGG CGTCTCCTCC TGTGGAGGAG 
AAGCGTCTAC TGCAGCCACT TTCCAGCACG CCCGTGACGC AGTTGCAGGC GGAGCCGGCC 
AAAAGAGTTC CCACCGCTGC CAGTGTCTCG GGCAGCAGTC GGAGCACCAC TCCGGTGCCC 
TCTGCCCGCA GCACCACCAC GCACACCACA ACAGCCACCC TGAGTTCCCA GCCAGCGGCT 
CAGCCGAGGA GAACCCACCT GGTGGAGGGA GTGCCTCAGA CATCCGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723930.1
CDS	109..2979
Translation	MMHKLKYRDKLKWLLALLVLIGTCFIQTRGQTRDPRFYSRPGVDYHWPNPGDPDYRTYTFNDRRYGHYQPNGYGANYPGRNPPGQYPQGMPNEDRFRFDPNDPNARTQFPGVLAGWREDLQGKQRRDSLTLERDVFVTTNYGQVQGFKVYMYDNPDPKSFYRPYHSTVDRVMGECSVFLGIPYALPPTFEGRFKPPRVHRGWQLLQAVDFGPACPQPVRYTGATKGIMDMDEDCLYLNVYSPKTGAGVAQKYPVMVYIHGGEFIRGASNLFQGHILASFYDVVVVTLNYRLGALGFLSTGDENSPGNYGILDQAMALRWVYDNIEFFNGDRNSITLFGPGAGGASAGLLMVAPQTRNIVRRVIAQSGSALADWALIQDKYRAQNTSRVLGQLLGCSIESSWKLVNCLRTGRSFYELGNAEFSPQVGSFPWGPVLDHNFTLPGDDWYEGWREKDWRFLTQTPETLIRAGKFNRNIQYMTGVTTQEAAFFVAQNESLSPYYELDGRFFDQKIREHVFRYNYTLNPNGVYEAIKYIYTFWPDPNNNTIIRDQYINMLSDLYYRAPVDQMVKLMLEQKVPVYMYVLNTTVEALNLPQWRKYPHDIERYFLTGAPFMDTEFFPKKEHLQRNMWTDNDRNMSHFFMQTYTNFARYGNPTPQQVLGMHFQRAYQGEIRYLNINTTYNSSILLNYRQTECAFWTQYLPTVIGVLVPTYPPTTEYWWEPKEPLQIAFWSMSVACFFLIVLVVICCIMWRNAKRQSDRFYDEDVFINGEGLEPEQDTRGVDNAHMVTNHHALRSRDNIYEYRDSPSTKTLASKAHTDTTSLRSPSSLAMTQKSSSQASLKSGISLKETNGHLVKQSERAATPRSQQNGSTAKVASPPVEEKRLLQPLSSTPVTQLQAEPAKRVPTAASVSGSSRSTTPVPSARSTTTHTTTATLSSQPAAQPRRTHLVEGVPQTSV

Target ORF information:

RefSeq Version	NM_165129.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gliotactin, transcript variant E (Gli), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165129.2

ORF Insert Sequence:

ATGATGCACA AATTGAAATA TCGCGATAAA TTAAAATGGC TTTTAGCCCT TCTTGTGCTG 
ATCGGCACTT GTTTTATTCA GACAAGGGGA CAAACAAGAG ATCCCAGATT TTATTCTCGG 
CCAGGCGTTG ACTACCATTG GCCAAATCCA GGCGATCCGG ATTACAGAAC CTACACGTTC 
AACGATCGCC GATATGGTCA TTATCAGCCA AATGGCTATG GAGCCAACTA TCCAGGCAGA 
AATCCACCGG GACAATATCC ACAGGGAATG CCGAATGAAG ATCGCTTTCG ATTTGACCCG 
AACGATCCGA ATGCGAGAAC CCAGTTTCCG GGAGTGCTGG CCGGATGGCG AGAGGATTTG 
CAGGGCAAGC AGCGGCGGGA TTCGTTGACC CTGGAGCGGG ATGTTTTCGT GACCACCAAC 
TATGGCCAGG TGCAGGGCTT TAAGGTGTAC ATGTACGATA ATCCAGATCC GAAGTCCTTC 
TATCGTCCCT ACCACTCGAC CGTGGATCGT GTGATGGGCG AGTGCTCGGT CTTCCTGGGC 
ATTCCCTACG CCCTGCCGCC CACCTTCGAG GGCAGGTTCA AGCCACCACG CGTCCATCGA 
GGCTGGCAGC TGCTGCAGGC CGTCGACTTT GGACCCGCCT GTCCACAGCC TGTGCGATAT 
ACGGGTGCCA CGAAAGGAAT CATGGACATG GACGAGGATT GCCTCTACTT GAACGTGTAT 
TCGCCGAAGA CTGGTGCTGG TGTGGCTCAA AAATACCCGG TTATGGTGTA CATCCATGGC 
GGCGAGTTCA TTCGTGGAGC CTCCAACCTA TTCCAGGGTC ATATTCTGGC CTCGTTCTAC 
GACGTGGTCG TGGTGACCCT GAATTACCGC CTTGGTGCCC TGGGATTCCT ATCGACGGGT 
GATGAGAACT CGCCCGGAAA CTACGGAATC CTCGATCAAG CGATGGCGCT ACGTTGGGTC 
TATGACAATA TTGAGTTCTT CAACGGCGAT CGGAATTCCA TCACTCTATT TGGTCCGGGA 
GCAGGAGGCG CCTCCGCTGG ACTCCTGATG GTGGCACCAC AGACGCGGAA CATTGTGCGT 
CGTGTGATCG CACAGTCCGG ATCGGCTCTA GCGGATTGGG CGCTCATCCA GGACAAGTAT 
CGCGCCCAGA ACACGAGTCG CGTGCTGGGA CAGCTGCTGG GCTGCTCCAT TGAATCGTCG 
TGGAAGTTGG TCAACTGCCT GCGCACCGGA CGCAGCTTCT ATGAGCTGGG AAACGCTGAG 
TTCTCTCCCC AGGTGGGCAG CTTTCCATGG GGTCCAGTTC TGGACCACAA CTTTACGTTG 
CCCGGCGACG ATTGGTACGA GGGATGGCGC GAAAAGGATT GGCGTTTCCT CACCCAAACG 
CCGGAAACCC TCATCCGTGC CGGTAAATTC AACCGGAATA TTCAGTACAT GACGGGCGTG 
ACCACACAGG AAGCGGCCTT TTTTGTGGCC CAAAACGAAT CCCTAAGTCC GTACTATGAA 
CTAGATGGAC GTTTCTTCGA TCAGAAAATA AGGGAACACG TTTTCCGCTA CAACTATACA 
CTTAATCCGA ACGGAGTTTA CGAGGCCATC AAGTACATAT ACACCTTCTG GCCGGATCCC 
AATAATAACA CCATAATCCG GGACCAGTAC ATAAACATGC TGAGTGATCT CTACTACCGA 
GCACCGGTGG ATCAAATGGT CAAGCTAATG CTGGAGCAGA AGGTACCCGT TTATATGTAC 
GTACTGAACA CCACTGTGGA GGCACTGAAT CTGCCACAGT GGCGAAAGTA TCCACACGAC 
ATCGAACGTT ATTTCCTCAC CGGAGCTCCC TTCATGGACA CCGAGTTCTT TCCCAAAAAG 
GAGCATCTGC AGCGCAATAT GTGGACGGAT AACGATCGCA ATATGAGTCA CTTTTTCATG 
CAGACCTACA CGAATTTTGC TAGATATGGC AATCCGACGC CGCAACAGGT GCTAGGCATG 
CATTTCCAGC GCGCATACCA GGGCGAGATT CGGTACTTGA ACATCAATAC CACGTACAAC 
TCCTCCATTC TACTCAACTA TCGGCAGACG GAGTGCGCCT TCTGGACGCA ATACTTGCCC 
ACAGTTATTG GAGTGCTGGT GCCCACTTAT CCACCCACCA CGGAGTATTG GTGGGAGCCC 
AAGGAGCCAC TACAGATCGC CTTTTGGAGC ATGTCGGTGG CCTGTTTCTT CCTCATAGTC 
CTGGTGGTCA TCTGCTGCAT CATGTGGCGC AATGCCAAGC GCCAATCGGA TCGCTTCTAT 
GACGAAGATG TCTTCATTAA TGGTGAGGGC TTGGAACCGG AACAGGATAC GCGTGGAGTG 
GACAATGCCC ACATGGTGAC CAACCATCAT GCCCTGCGCT CCAGGGATAA TATCTACGAG 
TACCGCGACT CTCCATCCAC CAAAACCTTG GCCAGCAAAG CGCACACGGA CACCACCTCG 
TTGCGCTCAC CCAGTTCGCT GGCCATGACC CAAAAGTCCA GCAGCCAGGC GTCCCTCAAG 
TCAGGGATCT CGCTCAAGGA AACCAATGGC CATTTGGTGA AGCAATCTGA AAGGGCAGCC 
ACGCCACGAT CCCAACAAAA TGGGTCCACC GCAAAGGTGG CGTCTCCTCC TGTGGAGGAG 
AAGCGTCTAC TGCAGCCACT TTCCAGCACG CCCGTGACGC AGTTGCAGGC GGAGCCGGCC 
AAAAGAGTTC CCACCGCTGC CAGTGTCTCG GGCAGCAGTC GGAGCACCAC TCCGGTGCCC 
TCTGCCCGCA GCACCACCAC GCACACCACA ACAGCCACCC TGAGTTCCCA GCCAGCGGCT 
CAGCCGAGGA GAACCCACCT GGTGGAGGGA GTGCCTCAGA CATCCGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gli cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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