CycE cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CycE
Entrez Gene ID	34924
Full Name	Cyclin E
Synonyms	3938,BG:DS07108.3,CDI7,CG3938,CYCE,Cdi7,Cyc,Cyc E,CycEI,CyclE,CyeE,D-CycE,DmCycE,DmcycE,DmcyclinE,Dmel\CG3938,br37,cdi7,cycE,cyclinE,cycline,dCycE,dm-cycE,fond,l(2)05206,l(2)35Dd,l(2)br37,l(2)k02514,l(2)k02602,l(2)k05007,l35Dd
General protein information	Preferred Names Cyclin E Names CG3938 gene product from transcript CG3938-RE CG3938-PA CG3938-PB CG3938-PC CG3938-PD CG3938-PE CG3938-PF CycE-PA CycE-PB CycE-PC CycE-PD CycE-PE CycE-PF Cyclin-dependent kinase interactor 7 cyclin cyclin E cyclin-E cyclinE cycline E dCyclinE fondue
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35D4-35D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_165123.3	NP_723924.1	cyclin E, isoform C
NM_165125.3	NP_723926.1	cyclin E, isoform E
NM_165124.3	NP_723925.1	cyclin E, isoform D
NM_057612.5	NP_476960.1	cyclin E, isoform B
NM_001259108.2	NP_001246037.1	cyclin E, isoform F
NM_057611.5	NP_476959.1	cyclin E, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	CCNE1	NP_001229.1
Danio rerio (zebrafish)	ccne1	NP_571070.1
Canis lupus familiaris (dog)	CCNE1	XP_005616959.1
Rattus norvegicus (Norway rat)	Ccne1	NP_001094291.1
Xenopus tropicalis (tropical clawed frog)	ccne1	NP_001016328.1
Pan troglodytes (chimpanzee)	CCNE1	XP_512559.4
Mus musculus (house mouse)	Ccne1	NP_031659.2
	CCNE1	XP_001084995.1
Bos taurus (cattle)	CCNE1	NP_001179705.1
Gallus gallus (chicken)	CCNE1	NP_001026529.1
Drosophila melanogaster (fruit fly)	CycE	NP_476960.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we show that Ewg repression of Cyclin E expression is required for the arrest of myoblast proliferation and initiate myoblast fusion and terminal differentiation
Title: Spatio-temporal coordination of cell cycle exit, fusion and differentiation of adult muscle precursors by Drosophila Erect wing (Ewg).

we provide genetic evidences, that the Ama-Nrt-Abl-pathway regulates CycE expression by altering the function of the Hippo effector Yorkie in embryonic NBs
Title: Cell-Autonomous and Non-cell-autonomous Function of Hox Genes Specify Segmental Neuroblast Identity in the Gnathal Region of the Embryonic CNS in Drosophila.

We further detected that reduced level of GRK2 induced a small cell cycle arrest at G2/M phase by enhancing the expression of cyclin A, B1, and E
Title: GRK2 negatively regulates IGF-1R signaling pathway and cyclins' expression in HepG2 cells.

CycE and Cdk2 are required for germline stem cell proliferation.
Title: Cyclin E controls Drosophila female germline stem cell maintenance independently of its role in proliferation by modulating responsiveness to niche signals.

p53-mediated regulation of ectopic neural stem cells (NSC) self-renewal through the Ago/Cyc E axis becomes particularly important when NSC homeostasis is perturbed.
Title: dp53 Restrains ectopic neural stem cell formation in the Drosophila brain in a non-apoptotic mechanism involving Archipelago and cyclin E.

The following CycE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CycE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16793	NM_057612.5 Latest version!	Drosophila melanogaster cyclin E, transcript variant B (CycE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14716	NM_001259108.2 Latest version!	Drosophila melanogaster cyclin E, transcript variant F (CycE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16791	NM_165124.3 Latest version!	Drosophila melanogaster cyclin E, transcript variant D (CycE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16791	NM_057611.5 Latest version!	Drosophila melanogaster cyclin E, transcript variant A (CycE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16791	NM_165125.3 Latest version!	Drosophila melanogaster cyclin E, transcript variant E (CycE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16791	NM_165123.3 Latest version!	Drosophila melanogaster cyclin E, transcript variant C (CycE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CycE ( NM_057612.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476960.1 Drosophila melanogaster cyclin E, transcript variant B (CycE), mRNA.

Price & Availability↑

CloneID	OFa16793
Clone ID Related Accession (Same CDS sequence)	NM_057612.5
Accession Version	NM_057612.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cyclin E, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057612.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTGG AACAGAAGCG CAAATTTATT GAAATGGACC CTGAACTCGG TTTTGAGCCT 
CCATCAGCTA AGCGCCAGCA GCGACTGCCC GCTTTGTACG GCAGCGAGCA GGGCAATCTG 
TCATCGGTTG CCTCCTCGGT CTACACCTCG CCCGTTGTCT CGGTTGATGG CCAGAGTACC 
CAGGAGCTGC TCAGCATACG TAGCTCACCG GCCGAGGATC TGTCGGAGGC GCCACACAGT 
CCGCTGCCGG ACAGCCCGGA CAGTCCGCCG AGTCCGGATC GTGGAAGCAA GCAGACGCCA 
GTGGTGGTGC GCTACGCTGC GGAGCAGGTG GTGACCAGTA CGGTGGTCAC GCAGAAGACG 
GAGGATGATG ACCTTTTGGA CGACAGCTGC GAGGACTACT CGTACGACGA GGATGATGAG 
GATGACGTTG AGGAGGAGGA CGACGACGTG GAGATCTACT CCTCCACAAT TTCTCCAGCC 
TCCTCCGGCT GCAGCCAGCA GCAGGCGGTG AATGGAGAGC GTACTCCCGG CCTGCCAAAA 
CATCAGGAGC AGATCCATCA TCCGGTCAGT GACCTGATGA TTAACATGCG GACGCCCATG 
TCGCCGGCTG TGGAGAACGG TCTGCGCCAG TGCCCACTTC CTGCGCTCGC CTGGGCTAAC 
GCCGCCGATG TTTGGCGCCT GATGTGTCAC CGGGATGAGC AGGACTCGCG TCTGCGCAGC 
ATTTCGATGC TGGAACAGCA TCCCGGACTG CAACCACGTA TGCGTGCCAT TCTCTTGGAC 
TGGTTGATCG AGGTCTGTGA AGTTTACAAG CTGCATCGGG AGACCTTCTA CTTGGCCGTC 
GACTACCTGG ATCGCTATTT GCATGTGGCG CATAAGGTGC AGAAGACGCA CTTGCAGTTG 
ATCGGCATCA CCTGCCTGTT TGTGGCCGCC AAGGTAGAGG AGATTTATCC GCCAAAGATC 
GGGGAGTTTG CTTATGTGAC GGATGGCGCC TGCACAGAGC GGGACATCCT TAACCACGAG 
AAGATTCTGC TGCAGGCGCT CGACTGGGAC ATCAGCCCCA TCACCATTAC CGGCTGGCTG 
GGCGTCTATA TGCAACTGAA TGTGAACAAC CGCACTCCGG CCTCGTTCTC CCAGATTGGC 
AGGCAAAAGA GCGCGGAGGC GGACGATGCC TTTATCTACC CGCAATTCTC TGGCTTTGAG 
TTTGTGCAAA CCTCACAGCT GCTAGATCTG TGCACCCTGG ACGTGGGCAT GGCCAACTAT 
TCCTACTCGG TGCTGGCAGC GGCCGCCATC AGTCATACAT TTAGTCGGGA GATGGCTTTA 
CGCTGTTCTG GTCTGGATTG GCAGGTGATT CAGCCTTGTG CTCGCTGGAT GGAGCCATTC 
TTCCGAGTGA TCTCCCAGAA GGCCCCCTAC CTGCAGCTGA ACGAGCAGAA TGAGCAAGTC 
AGCAACAAAT TTGGCCTGGG ACTAATCTGC CCCAATATTG TCACCGACGA CTCGCACATT 
ATCCAAACGC ACACAACTAC CATGGATATG TATGACGAAG TGCTTATGGC CCAGGATGCG 
GCGCATGCCA TGCGCGCTAG GATTCAAGCT TCCCCGGCCA CCGCGCTGCG CGCTCCCGAA 
AGTCTTCTGA CGCCTCCCGC CTCTAGTCAC AAGCCGGACG AGTACCTGGG CGATGAGGGC 
GATGAGACGG GGGCCAGGAG CGGCATCTCC TCCACAACCA CATGCTGCAA CACTGCCGCC 
AGTAACAAGG GCGGCAAGAG CAGCAGCAAC AATTCGGTGA CCAGCTGCAG CAGTAGAAGC 
AATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476960.1
CDS	748..2556
Translation	MKLEQKRKFIEMDPELGFEPPSAKRQQRLPALYGSEQGNLSSVASSVYTSPVVSVDGQSTQELLSIRSSPAEDLSEAPHSPLPDSPDSPPSPDRGSKQTPVVVRYAAEQVVTSTVVTQKTEDDDLLDDSCEDYSYDEDDEDDVEEEDDDVEIYSSTISPASSGCSQQQAVNGERTPGLPKHQEQIHHPVSDLMINMRTPMSPAVENGLRQCPLPALAWANAADVWRLMCHRDEQDSRLRSISMLEQHPGLQPRMRAILLDWLIEVCEVYKLHRETFYLAVDYLDRYLHVAHKVQKTHLQLIGITCLFVAAKVEEIYPPKIGEFAYVTDGACTERDILNHEKILLQALDWDISPITITGWLGVYMQLNVNNRTPASFSQIGRQKSAEADDAFIYPQFSGFEFVQTSQLLDLCTLDVGMANYSYSVLAAAAISHTFSREMALRCSGLDWQVIQPCARWMEPFFRVISQKAPYLQLNEQNEQVSNKFGLGLICPNIVTDDSHIIQTHTTTMDMYDEVLMAQDAAHAMRARIQASPATALRAPESLLTPPASSHKPDEYLGDEGDETGARSGISSTTTCCNTAASNKGGKSSSNNSVTSCSSRSNP

Target ORF information:

RefSeq Version	NM_057612.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cyclin E, transcript variant B (CycE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057612.5

ORF Insert Sequence:

ATGAAGTTGG AACAGAAGCG CAAATTTATT GAAATGGACC CTGAACTCGG TTTTGAGCCT 
CCATCAGCTA AGCGCCAGCA GCGACTGCCC GCTTTGTACG GCAGCGAGCA GGGCAATCTG 
TCATCGGTTG CCTCCTCGGT CTACACCTCG CCCGTTGTCT CGGTTGATGG CCAGAGTACC 
CAGGAGCTGC TCAGCATACG TAGCTCACCG GCCGAGGATC TGTCGGAGGC GCCACACAGT 
CCGCTGCCGG ACAGCCCGGA CAGTCCGCCG AGTCCGGATC GTGGAAGCAA GCAGACGCCA 
GTGGTGGTGC GCTACGCTGC GGAGCAGGTG GTGACCAGTA CGGTGGTCAC GCAGAAGACG 
GAGGATGATG ACCTTTTGGA CGACAGCTGC GAGGACTACT CGTACGACGA GGATGATGAG 
GATGACGTTG AGGAGGAGGA CGACGACGTG GAGATCTACT CCTCCACAAT TTCTCCAGCC 
TCCTCCGGCT GCAGCCAGCA GCAGGCGGTG AATGGAGAGC GTACTCCCGG CCTGCCAAAA 
CATCAGGAGC AGATCCATCA TCCGGTCAGT GACCTGATGA TTAACATGCG GACGCCCATG 
TCGCCGGCTG TGGAGAACGG TCTGCGCCAG TGCCCACTTC CTGCGCTCGC CTGGGCTAAC 
GCCGCCGATG TTTGGCGCCT GATGTGTCAC CGGGATGAGC AGGACTCGCG TCTGCGCAGC 
ATTTCGATGC TGGAACAGCA TCCCGGACTG CAACCACGTA TGCGTGCCAT TCTCTTGGAC 
TGGTTGATCG AGGTCTGTGA AGTTTACAAG CTGCATCGGG AGACCTTCTA CTTGGCCGTC 
GACTACCTGG ATCGCTATTT GCATGTGGCG CATAAGGTGC AGAAGACGCA CTTGCAGTTG 
ATCGGCATCA CCTGCCTGTT TGTGGCCGCC AAGGTAGAGG AGATTTATCC GCCAAAGATC 
GGGGAGTTTG CTTATGTGAC GGATGGCGCC TGCACAGAGC GGGACATCCT TAACCACGAG 
AAGATTCTGC TGCAGGCGCT CGACTGGGAC ATCAGCCCCA TCACCATTAC CGGCTGGCTG 
GGCGTCTATA TGCAACTGAA TGTGAACAAC CGCACTCCGG CCTCGTTCTC CCAGATTGGC 
AGGCAAAAGA GCGCGGAGGC GGACGATGCC TTTATCTACC CGCAATTCTC TGGCTTTGAG 
TTTGTGCAAA CCTCACAGCT GCTAGATCTG TGCACCCTGG ACGTGGGCAT GGCCAACTAT 
TCCTACTCGG TGCTGGCAGC GGCCGCCATC AGTCATACAT TTAGTCGGGA GATGGCTTTA 
CGCTGTTCTG GTCTGGATTG GCAGGTGATT CAGCCTTGTG CTCGCTGGAT GGAGCCATTC 
TTCCGAGTGA TCTCCCAGAA GGCCCCCTAC CTGCAGCTGA ACGAGCAGAA TGAGCAAGTC 
AGCAACAAAT TTGGCCTGGG ACTAATCTGC CCCAATATTG TCACCGACGA CTCGCACATT 
ATCCAAACGC ACACAACTAC CATGGATATG TATGACGAAG TGCTTATGGC CCAGGATGCG 
GCGCATGCCA TGCGCGCTAG GATTCAAGCT TCCCCGGCCA CCGCGCTGCG CGCTCCCGAA 
AGTCTTCTGA CGCCTCCCGC CTCTAGTCAC AAGCCGGACG AGTACCTGGG CGATGAGGGC 
GATGAGACGG GGGCCAGGAG CGGCATCTCC TCCACAACCA CATGCTGCAA CACTGCCGCC 
AGTAACAAGG GCGGCAAGAG CAGCAGCAAC AATTCGGTGA CCAGCTGCAG CAGTAGAAGC 
AATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CycE ( NM_001259108.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246037.1 Drosophila melanogaster cyclin E, transcript variant F (CycE), mRNA.

Price & Availability↑

CloneID	OFa14716
Clone ID Related Accession (Same CDS sequence)	NM_001259108.2
Accession Version	NM_001259108.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cyclin E, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001259108.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAAGCA TTTTTCCGCT TACTAGAAGT GTTTGTTCTA CCAGCAGCAC TGAGCCAAAT 
GGCAGCATAG TCACAACCGC CCCCAGCAAC GGAGAAGTGA GCAGCAGCAT AGTCGTCGTC 
GTCAGCAGCA GTAGTATCAG TAGCAGCAGC GATAGCCCAA TCGCTATCCT TCCACATCCA 
GATCCAATCC CATCAACATC GTTCTCTTCA GCGTCGCAAC GAAGTGAAGA GGAGCTACCA 
GGAACCTCAG CAGCCTCCAG AACCGACGAG ATGTGCTCTT GTGATAGCCA GAATCTTGCA 
GCATCCACAG CCGCGACTTC CAATGGCAAT AAACGTAAAC GGCGTTTAAG CAGCGATTCA 
AACGAGGACC CTGAACTCGG TTTTGAGCCT CCATCAGCTA AGCGCCAGCA GCGACTGCCC 
GCTTTGTACG GCAGCGAGCA GGGCAATCTG TCATCGGTTG CCTCCTCGGT CTACACCTCG 
CCCGTTGTCT CGGTTGATGG CCAGAGTACC CAGGAGCTGC TCAGCATACG TAGCTCACCG 
GCCGAGGATC TGTCGGAGGC GCCACACAGT CCGCTGCCGG ACAGCCCGGA CAGTCCGCCG 
AGTCCGGATC GTGGAAGCAA GCAGACGCCA GTGGTGGTGC GCTACGCTGC GGAGCAGGTG 
GTGACCAGTA CGGTGGTCAC GCAGAAGACG GAGGATGATG ACCTTTTGGA CGACAGCTGC 
GAGGACTACT CGTACGACGA GGATGATGAG GATGACGTTG AGGAGGAGGA CGACGACGTG 
GAGATCTACT CCTCCACAAT TTCTCCAGCC TCCTCCGGCT GCAGCCAGCA GCAGGCGGTG 
AATGGAGAGC GTACTCCCGG CCTGCCAAAA CATCAGGAGC AGATCCATCA TCCGGTCAGT 
GACCTGATGA TTAACATGCG GACGCCCATG TCGCCGGCTG TGGAGAACGG TCTGCGCCAG 
TGCCCACTTC CTGCGCTCGC CTGGGCTAAC GCCGCCGATG TTTGGCGCCT GATGTGTCAC 
CGGGATGAGC AGGACTCGCG TCTGCGCAGC ATTTCGATGC TGGAACAGCA TCCCGGACTG 
CAACCACGTA TGCGTGCCAT TCTCTTGGAC TGGTTGATCG AGGTCTGTGA AGTTTACAAG 
CTGCATCGGG AGACCTTCTA CTTGGCCGTC GACTACCTGG ATCGCTATTT GCATGTGGCG 
CATAAGGTGC AGAAGACGCA CTTGCAGTTG ATCGGCATCA CCTGCCTGTT TGTGGCCGCC 
AAGGTAGAGG AGATTTATCC GCCAAAGATC GGGGAGTTTG CTTATGTGAC GGATGGCGCC 
TGCACAGAGC GGGACATCCT TAACCACGAG AAGATTCTGC TGCAGGCGCT CGACTGGGAC 
ATCAGCCCCA TCACCATTAC CGGCTGGCTG GGCGTCTATA TGCAACTGAA TGTGAACAAC 
CGCACTCCGG CCTCGTTCTC CCAGATTGGC AGGCAAAAGA GCGCGGAGGC GGACGATGCC 
TTTATCTACC CGCAATTCTC TGGCTTTGAG TTTGTGCAAA CCTCACAGCT GCTAGATCTG 
TGCACCCTGG ACGTGGGCAT GGCCAACTAT TCCTACTCGG TGCTGGCAGC GGCCGCCATC 
AGTCATACAT TTAGTCGGGA GATGGCTTTA CGCTGTTCTG GTCTGGATTG GCAGGTGATT 
CAGCCTTGTG CTCGCTGGAT GGAGCCATTC TTCCGAGTGA TCTCCCAGAA GGCCCCCTAC 
CTGCAGCTGA ACGAGCAGAA TGAGCAAGTC AGCAACAAAT TTGGCCTGGG ACTAATCTGC 
CCCAATATTG TCACCGACGA CTCGCACATT ATCCAAACGC ACACAACTAC CATGGATATG 
TATGACGAAG TGCTTATGGC CCAGGATGCG GCGCATGCCA TGCGCGCTAG GATTCAAGCT 
TCCCCGGCCA CCGCGCTGCG CGCTCCCGAA AGTCTTCTGA CGCCTCCCGC CTCTAGTCAC 
AAGCCGGACG AGTACCTGGG CGATGAGGGC GATGAGACGG GGGCCAGGAG CGGCATCTCC 
TCCACAACCA CATGCTGCAA CACTGCCGCC AGTAACAAGG GCGGCAAGAG CAGCAGCAAC 
AATTCGGTGA CCAGCTGCAG CAGTAGAAGC AATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246037.1
CDS	128..2266
Translation	MLSIFPLTRSVCSTSSTEPNGSIVTTAPSNGEVSSSIVVVVSSSSISSSSDSPIAILPHPDPIPSTSFSSASQRSEEELPGTSAASRTDEMCSCDSQNLAASTAATSNGNKRKRRLSSDSNEDPELGFEPPSAKRQQRLPALYGSEQGNLSSVASSVYTSPVVSVDGQSTQELLSIRSSPAEDLSEAPHSPLPDSPDSPPSPDRGSKQTPVVVRYAAEQVVTSTVVTQKTEDDDLLDDSCEDYSYDEDDEDDVEEEDDDVEIYSSTISPASSGCSQQQAVNGERTPGLPKHQEQIHHPVSDLMINMRTPMSPAVENGLRQCPLPALAWANAADVWRLMCHRDEQDSRLRSISMLEQHPGLQPRMRAILLDWLIEVCEVYKLHRETFYLAVDYLDRYLHVAHKVQKTHLQLIGITCLFVAAKVEEIYPPKIGEFAYVTDGACTERDILNHEKILLQALDWDISPITITGWLGVYMQLNVNNRTPASFSQIGRQKSAEADDAFIYPQFSGFEFVQTSQLLDLCTLDVGMANYSYSVLAAAAISHTFSREMALRCSGLDWQVIQPCARWMEPFFRVISQKAPYLQLNEQNEQVSNKFGLGLICPNIVTDDSHIIQTHTTTMDMYDEVLMAQDAAHAMRARIQASPATALRAPESLLTPPASSHKPDEYLGDEGDETGARSGISSTTTCCNTAASNKGGKSSSNNSVTSCSSRSNP

Target ORF information:

RefSeq Version	NM_001259108.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cyclin E, transcript variant F (CycE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259108.2

ORF Insert Sequence:

ATGTTAAGCA TTTTTCCGCT TACTAGAAGT GTTTGTTCTA CCAGCAGCAC TGAGCCAAAT 
GGCAGCATAG TCACAACCGC CCCCAGCAAC GGAGAAGTGA GCAGCAGCAT AGTCGTCGTC 
GTCAGCAGCA GTAGTATCAG TAGCAGCAGC GATAGCCCAA TCGCTATCCT TCCACATCCA 
GATCCAATCC CATCAACATC GTTCTCTTCA GCGTCGCAAC GAAGTGAAGA GGAGCTACCA 
GGAACCTCAG CAGCCTCCAG AACCGACGAG ATGTGCTCTT GTGATAGCCA GAATCTTGCA 
GCATCCACAG CCGCGACTTC CAATGGCAAT AAACGTAAAC GGCGTTTAAG CAGCGATTCA 
AACGAGGACC CTGAACTCGG TTTTGAGCCT CCATCAGCTA AGCGCCAGCA GCGACTGCCC 
GCTTTGTACG GCAGCGAGCA GGGCAATCTG TCATCGGTTG CCTCCTCGGT CTACACCTCG 
CCCGTTGTCT CGGTTGATGG CCAGAGTACC CAGGAGCTGC TCAGCATACG TAGCTCACCG 
GCCGAGGATC TGTCGGAGGC GCCACACAGT CCGCTGCCGG ACAGCCCGGA CAGTCCGCCG 
AGTCCGGATC GTGGAAGCAA GCAGACGCCA GTGGTGGTGC GCTACGCTGC GGAGCAGGTG 
GTGACCAGTA CGGTGGTCAC GCAGAAGACG GAGGATGATG ACCTTTTGGA CGACAGCTGC 
GAGGACTACT CGTACGACGA GGATGATGAG GATGACGTTG AGGAGGAGGA CGACGACGTG 
GAGATCTACT CCTCCACAAT TTCTCCAGCC TCCTCCGGCT GCAGCCAGCA GCAGGCGGTG 
AATGGAGAGC GTACTCCCGG CCTGCCAAAA CATCAGGAGC AGATCCATCA TCCGGTCAGT 
GACCTGATGA TTAACATGCG GACGCCCATG TCGCCGGCTG TGGAGAACGG TCTGCGCCAG 
TGCCCACTTC CTGCGCTCGC CTGGGCTAAC GCCGCCGATG TTTGGCGCCT GATGTGTCAC 
CGGGATGAGC AGGACTCGCG TCTGCGCAGC ATTTCGATGC TGGAACAGCA TCCCGGACTG 
CAACCACGTA TGCGTGCCAT TCTCTTGGAC TGGTTGATCG AGGTCTGTGA AGTTTACAAG 
CTGCATCGGG AGACCTTCTA CTTGGCCGTC GACTACCTGG ATCGCTATTT GCATGTGGCG 
CATAAGGTGC AGAAGACGCA CTTGCAGTTG ATCGGCATCA CCTGCCTGTT TGTGGCCGCC 
AAGGTAGAGG AGATTTATCC GCCAAAGATC GGGGAGTTTG CTTATGTGAC GGATGGCGCC 
TGCACAGAGC GGGACATCCT TAACCACGAG AAGATTCTGC TGCAGGCGCT CGACTGGGAC 
ATCAGCCCCA TCACCATTAC CGGCTGGCTG GGCGTCTATA TGCAACTGAA TGTGAACAAC 
CGCACTCCGG CCTCGTTCTC CCAGATTGGC AGGCAAAAGA GCGCGGAGGC GGACGATGCC 
TTTATCTACC CGCAATTCTC TGGCTTTGAG TTTGTGCAAA CCTCACAGCT GCTAGATCTG 
TGCACCCTGG ACGTGGGCAT GGCCAACTAT TCCTACTCGG TGCTGGCAGC GGCCGCCATC 
AGTCATACAT TTAGTCGGGA GATGGCTTTA CGCTGTTCTG GTCTGGATTG GCAGGTGATT 
CAGCCTTGTG CTCGCTGGAT GGAGCCATTC TTCCGAGTGA TCTCCCAGAA GGCCCCCTAC 
CTGCAGCTGA ACGAGCAGAA TGAGCAAGTC AGCAACAAAT TTGGCCTGGG ACTAATCTGC 
CCCAATATTG TCACCGACGA CTCGCACATT ATCCAAACGC ACACAACTAC CATGGATATG 
TATGACGAAG TGCTTATGGC CCAGGATGCG GCGCATGCCA TGCGCGCTAG GATTCAAGCT 
TCCCCGGCCA CCGCGCTGCG CGCTCCCGAA AGTCTTCTGA CGCCTCCCGC CTCTAGTCAC 
AAGCCGGACG AGTACCTGGG CGATGAGGGC GATGAGACGG GGGCCAGGAG CGGCATCTCC 
TCCACAACCA CATGCTGCAA CACTGCCGCC AGTAACAAGG GCGGCAAGAG CAGCAGCAAC 
AATTCGGTGA CCAGCTGCAG CAGTAGAAGC AATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CycE ( NM_165123.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723924.1 Drosophila melanogaster cyclin E, transcript variant C (CycE), mRNA.

Price & Availability↑

CloneID	OFa16791
Clone ID Related Accession (Same CDS sequence)	NM_165123.3 , NM_165125.3 , NM_165124.3 , NM_057611.5
Accession Version	NM_165123.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cyclin E, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165123.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTTAA ATGCCAAGAG TGTTTGTTCT ACCAGCAGCA CTGAGCCAAA TGGCAGCATA 
GTCACAACCG CCCCCAGCAA CGGAGAAGTG AGCAGCAGCA TAGTCGTCGT CGTCAGCAGC 
AGTAGTATCA GTAGCAGCAG CGATAGCCCA ATCGCTATCC TTCCACATCC AGATCCAATC 
CCATCAACAT CGTTCTCTTC AGCGTCGCAA CGAAGTGAAG AGGAGCTACC AGGAACCTCA 
GCAGCCTCCA GAACCGACGA GATGTGCTCT TGTGATAGCC AGAATCTTGC AGCATCCACA 
GCCGCGACTT CCAATGGCAA TAAACGTAAA CGGCGTTTAA GCAGCGATTC AAACGAGGAC 
CCTGAACTCG GTTTTGAGCC TCCATCAGCT AAGCGCCAGC AGCGACTGCC CGCTTTGTAC 
GGCAGCGAGC AGGGCAATCT GTCATCGGTT GCCTCCTCGG TCTACACCTC GCCCGTTGTC 
TCGGTTGATG GCCAGAGTAC CCAGGAGCTG CTCAGCATAC GTAGCTCACC GGCCGAGGAT 
CTGTCGGAGG CGCCACACAG TCCGCTGCCG GACAGCCCGG ACAGTCCGCC GAGTCCGGAT 
CGTGGAAGCA AGCAGACGCC AGTGGTGGTG CGCTACGCTG CGGAGCAGGT GGTGACCAGT 
ACGGTGGTCA CGCAGAAGAC GGAGGATGAT GACCTTTTGG ACGACAGCTG CGAGGACTAC 
TCGTACGACG AGGATGATGA GGATGACGTT GAGGAGGAGG ACGACGACGT GGAGATCTAC 
TCCTCCACAA TTTCTCCAGC CTCCTCCGGC TGCAGCCAGC AGCAGGCGGT GAATGGAGAG 
CGTACTCCCG GCCTGCCAAA ACATCAGGAG CAGATCCATC ATCCGGTCAG TGACCTGATG 
ATTAACATGC GGACGCCCAT GTCGCCGGCT GTGGAGAACG GTCTGCGCCA GTGCCCACTT 
CCTGCGCTCG CCTGGGCTAA CGCCGCCGAT GTTTGGCGCC TGATGTGTCA CCGGGATGAG 
CAGGACTCGC GTCTGCGCAG CATTTCGATG CTGGAACAGC ATCCCGGACT GCAACCACGT 
ATGCGTGCCA TTCTCTTGGA CTGGTTGATC GAGGTCTGTG AAGTTTACAA GCTGCATCGG 
GAGACCTTCT ACTTGGCCGT CGACTACCTG GATCGCTATT TGCATGTGGC GCATAAGGTG 
CAGAAGACGC ACTTGCAGTT GATCGGCATC ACCTGCCTGT TTGTGGCCGC CAAGGTAGAG 
GAGATTTATC CGCCAAAGAT CGGGGAGTTT GCTTATGTGA CGGATGGCGC CTGCACAGAG 
CGGGACATCC TTAACCACGA GAAGATTCTG CTGCAGGCGC TCGACTGGGA CATCAGCCCC 
ATCACCATTA CCGGCTGGCT GGGCGTCTAT ATGCAACTGA ATGTGAACAA CCGCACTCCG 
GCCTCGTTCT CCCAGATTGG CAGGCAAAAG AGCGCGGAGG CGGACGATGC CTTTATCTAC 
CCGCAATTCT CTGGCTTTGA GTTTGTGCAA ACCTCACAGC TGCTAGATCT GTGCACCCTG 
GACGTGGGCA TGGCCAACTA TTCCTACTCG GTGCTGGCAG CGGCCGCCAT CAGTCATACA 
TTTAGTCGGG AGATGGCTTT ACGCTGTTCT GGTCTGGATT GGCAGGTGAT TCAGCCTTGT 
GCTCGCTGGA TGGAGCCATT CTTCCGAGTG ATCTCCCAGA AGGCCCCCTA CCTGCAGCTG 
AACGAGCAGA ATGAGCAAGT CAGCAACAAA TTTGGCCTGG GACTAATCTG CCCCAATATT 
GTCACCGACG ACTCGCACAT TATCCAAACG CACACAACTA CCATGGATAT GTATGACGAA 
GTGCTTATGG CCCAGGATGC GGCGCATGCC ATGCGCGCTA GGATTCAAGC TTCCCCGGCC 
ACCGCGCTGC GCGCTCCCGA AAGTCTTCTG ACGCCTCCCG CCTCTAGTCA CAAGCCGGAC 
GAGTACCTGG GCGATGAGGG CGATGAGACG GGGGCCAGGA GCGGCATCTC CTCCACAACC 
ACATGCTGCA ACACTGCCGC CAGTAACAAG GGCGGCAAGA GCAGCAGCAA CAATTCGGTG 
ACCAGCTGCA GCAGTAGAAG CAATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723924.1
CDS	265..2394
Translation	MGLNAKSVCSTSSTEPNGSIVTTAPSNGEVSSSIVVVVSSSSISSSSDSPIAILPHPDPIPSTSFSSASQRSEEELPGTSAASRTDEMCSCDSQNLAASTAATSNGNKRKRRLSSDSNEDPELGFEPPSAKRQQRLPALYGSEQGNLSSVASSVYTSPVVSVDGQSTQELLSIRSSPAEDLSEAPHSPLPDSPDSPPSPDRGSKQTPVVVRYAAEQVVTSTVVTQKTEDDDLLDDSCEDYSYDEDDEDDVEEEDDDVEIYSSTISPASSGCSQQQAVNGERTPGLPKHQEQIHHPVSDLMINMRTPMSPAVENGLRQCPLPALAWANAADVWRLMCHRDEQDSRLRSISMLEQHPGLQPRMRAILLDWLIEVCEVYKLHRETFYLAVDYLDRYLHVAHKVQKTHLQLIGITCLFVAAKVEEIYPPKIGEFAYVTDGACTERDILNHEKILLQALDWDISPITITGWLGVYMQLNVNNRTPASFSQIGRQKSAEADDAFIYPQFSGFEFVQTSQLLDLCTLDVGMANYSYSVLAAAAISHTFSREMALRCSGLDWQVIQPCARWMEPFFRVISQKAPYLQLNEQNEQVSNKFGLGLICPNIVTDDSHIIQTHTTTMDMYDEVLMAQDAAHAMRARIQASPATALRAPESLLTPPASSHKPDEYLGDEGDETGARSGISSTTTCCNTAASNKGGKSSSNNSVTSCSSRSNP

Target ORF information:

RefSeq Version	NM_165123.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cyclin E, transcript variant C (CycE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165123.3

ORF Insert Sequence:

ATGGGTTTAA ATGCCAAGAG TGTTTGTTCT ACCAGCAGCA CTGAGCCAAA TGGCAGCATA 
GTCACAACCG CCCCCAGCAA CGGAGAAGTG AGCAGCAGCA TAGTCGTCGT CGTCAGCAGC 
AGTAGTATCA GTAGCAGCAG CGATAGCCCA ATCGCTATCC TTCCACATCC AGATCCAATC 
CCATCAACAT CGTTCTCTTC AGCGTCGCAA CGAAGTGAAG AGGAGCTACC AGGAACCTCA 
GCAGCCTCCA GAACCGACGA GATGTGCTCT TGTGATAGCC AGAATCTTGC AGCATCCACA 
GCCGCGACTT CCAATGGCAA TAAACGTAAA CGGCGTTTAA GCAGCGATTC AAACGAGGAC 
CCTGAACTCG GTTTTGAGCC TCCATCAGCT AAGCGCCAGC AGCGACTGCC CGCTTTGTAC 
GGCAGCGAGC AGGGCAATCT GTCATCGGTT GCCTCCTCGG TCTACACCTC GCCCGTTGTC 
TCGGTTGATG GCCAGAGTAC CCAGGAGCTG CTCAGCATAC GTAGCTCACC GGCCGAGGAT 
CTGTCGGAGG CGCCACACAG TCCGCTGCCG GACAGCCCGG ACAGTCCGCC GAGTCCGGAT 
CGTGGAAGCA AGCAGACGCC AGTGGTGGTG CGCTACGCTG CGGAGCAGGT GGTGACCAGT 
ACGGTGGTCA CGCAGAAGAC GGAGGATGAT GACCTTTTGG ACGACAGCTG CGAGGACTAC 
TCGTACGACG AGGATGATGA GGATGACGTT GAGGAGGAGG ACGACGACGT GGAGATCTAC 
TCCTCCACAA TTTCTCCAGC CTCCTCCGGC TGCAGCCAGC AGCAGGCGGT GAATGGAGAG 
CGTACTCCCG GCCTGCCAAA ACATCAGGAG CAGATCCATC ATCCGGTCAG TGACCTGATG 
ATTAACATGC GGACGCCCAT GTCGCCGGCT GTGGAGAACG GTCTGCGCCA GTGCCCACTT 
CCTGCGCTCG CCTGGGCTAA CGCCGCCGAT GTTTGGCGCC TGATGTGTCA CCGGGATGAG 
CAGGACTCGC GTCTGCGCAG CATTTCGATG CTGGAACAGC ATCCCGGACT GCAACCACGT 
ATGCGTGCCA TTCTCTTGGA CTGGTTGATC GAGGTCTGTG AAGTTTACAA GCTGCATCGG 
GAGACCTTCT ACTTGGCCGT CGACTACCTG GATCGCTATT TGCATGTGGC GCATAAGGTG 
CAGAAGACGC ACTTGCAGTT GATCGGCATC ACCTGCCTGT TTGTGGCCGC CAAGGTAGAG 
GAGATTTATC CGCCAAAGAT CGGGGAGTTT GCTTATGTGA CGGATGGCGC CTGCACAGAG 
CGGGACATCC TTAACCACGA GAAGATTCTG CTGCAGGCGC TCGACTGGGA CATCAGCCCC 
ATCACCATTA CCGGCTGGCT GGGCGTCTAT ATGCAACTGA ATGTGAACAA CCGCACTCCG 
GCCTCGTTCT CCCAGATTGG CAGGCAAAAG AGCGCGGAGG CGGACGATGC CTTTATCTAC 
CCGCAATTCT CTGGCTTTGA GTTTGTGCAA ACCTCACAGC TGCTAGATCT GTGCACCCTG 
GACGTGGGCA TGGCCAACTA TTCCTACTCG GTGCTGGCAG CGGCCGCCAT CAGTCATACA 
TTTAGTCGGG AGATGGCTTT ACGCTGTTCT GGTCTGGATT GGCAGGTGAT TCAGCCTTGT 
GCTCGCTGGA TGGAGCCATT CTTCCGAGTG ATCTCCCAGA AGGCCCCCTA CCTGCAGCTG 
AACGAGCAGA ATGAGCAAGT CAGCAACAAA TTTGGCCTGG GACTAATCTG CCCCAATATT 
GTCACCGACG ACTCGCACAT TATCCAAACG CACACAACTA CCATGGATAT GTATGACGAA 
GTGCTTATGG CCCAGGATGC GGCGCATGCC ATGCGCGCTA GGATTCAAGC TTCCCCGGCC 
ACCGCGCTGC GCGCTCCCGA AAGTCTTCTG ACGCCTCCCG CCTCTAGTCA CAAGCCGGAC 
GAGTACCTGG GCGATGAGGG CGATGAGACG GGGGCCAGGA GCGGCATCTC CTCCACAACC 
ACATGCTGCA ACACTGCCGC CAGTAACAAG GGCGGCAAGA GCAGCAGCAA CAATTCGGTG 
ACCAGCTGCA GCAGTAGAAG CAATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CycE ( NM_165125.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723926.1 Drosophila melanogaster cyclin E, transcript variant E (CycE), mRNA.

Price & Availability↑

CloneID	OFa16791
Clone ID Related Accession (Same CDS sequence)	NM_165123.3 , NM_165125.3 , NM_165124.3 , NM_057611.5
Accession Version	NM_165125.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cyclin E, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165125.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTTAA ATGCCAAGAG TGTTTGTTCT ACCAGCAGCA CTGAGCCAAA TGGCAGCATA 
GTCACAACCG CCCCCAGCAA CGGAGAAGTG AGCAGCAGCA TAGTCGTCGT CGTCAGCAGC 
AGTAGTATCA GTAGCAGCAG CGATAGCCCA ATCGCTATCC TTCCACATCC AGATCCAATC 
CCATCAACAT CGTTCTCTTC AGCGTCGCAA CGAAGTGAAG AGGAGCTACC AGGAACCTCA 
GCAGCCTCCA GAACCGACGA GATGTGCTCT TGTGATAGCC AGAATCTTGC AGCATCCACA 
GCCGCGACTT CCAATGGCAA TAAACGTAAA CGGCGTTTAA GCAGCGATTC AAACGAGGAC 
CCTGAACTCG GTTTTGAGCC TCCATCAGCT AAGCGCCAGC AGCGACTGCC CGCTTTGTAC 
GGCAGCGAGC AGGGCAATCT GTCATCGGTT GCCTCCTCGG TCTACACCTC GCCCGTTGTC 
TCGGTTGATG GCCAGAGTAC CCAGGAGCTG CTCAGCATAC GTAGCTCACC GGCCGAGGAT 
CTGTCGGAGG CGCCACACAG TCCGCTGCCG GACAGCCCGG ACAGTCCGCC GAGTCCGGAT 
CGTGGAAGCA AGCAGACGCC AGTGGTGGTG CGCTACGCTG CGGAGCAGGT GGTGACCAGT 
ACGGTGGTCA CGCAGAAGAC GGAGGATGAT GACCTTTTGG ACGACAGCTG CGAGGACTAC 
TCGTACGACG AGGATGATGA GGATGACGTT GAGGAGGAGG ACGACGACGT GGAGATCTAC 
TCCTCCACAA TTTCTCCAGC CTCCTCCGGC TGCAGCCAGC AGCAGGCGGT GAATGGAGAG 
CGTACTCCCG GCCTGCCAAA ACATCAGGAG CAGATCCATC ATCCGGTCAG TGACCTGATG 
ATTAACATGC GGACGCCCAT GTCGCCGGCT GTGGAGAACG GTCTGCGCCA GTGCCCACTT 
CCTGCGCTCG CCTGGGCTAA CGCCGCCGAT GTTTGGCGCC TGATGTGTCA CCGGGATGAG 
CAGGACTCGC GTCTGCGCAG CATTTCGATG CTGGAACAGC ATCCCGGACT GCAACCACGT 
ATGCGTGCCA TTCTCTTGGA CTGGTTGATC GAGGTCTGTG AAGTTTACAA GCTGCATCGG 
GAGACCTTCT ACTTGGCCGT CGACTACCTG GATCGCTATT TGCATGTGGC GCATAAGGTG 
CAGAAGACGC ACTTGCAGTT GATCGGCATC ACCTGCCTGT TTGTGGCCGC CAAGGTAGAG 
GAGATTTATC CGCCAAAGAT CGGGGAGTTT GCTTATGTGA CGGATGGCGC CTGCACAGAG 
CGGGACATCC TTAACCACGA GAAGATTCTG CTGCAGGCGC TCGACTGGGA CATCAGCCCC 
ATCACCATTA CCGGCTGGCT GGGCGTCTAT ATGCAACTGA ATGTGAACAA CCGCACTCCG 
GCCTCGTTCT CCCAGATTGG CAGGCAAAAG AGCGCGGAGG CGGACGATGC CTTTATCTAC 
CCGCAATTCT CTGGCTTTGA GTTTGTGCAA ACCTCACAGC TGCTAGATCT GTGCACCCTG 
GACGTGGGCA TGGCCAACTA TTCCTACTCG GTGCTGGCAG CGGCCGCCAT CAGTCATACA 
TTTAGTCGGG AGATGGCTTT ACGCTGTTCT GGTCTGGATT GGCAGGTGAT TCAGCCTTGT 
GCTCGCTGGA TGGAGCCATT CTTCCGAGTG ATCTCCCAGA AGGCCCCCTA CCTGCAGCTG 
AACGAGCAGA ATGAGCAAGT CAGCAACAAA TTTGGCCTGG GACTAATCTG CCCCAATATT 
GTCACCGACG ACTCGCACAT TATCCAAACG CACACAACTA CCATGGATAT GTATGACGAA 
GTGCTTATGG CCCAGGATGC GGCGCATGCC ATGCGCGCTA GGATTCAAGC TTCCCCGGCC 
ACCGCGCTGC GCGCTCCCGA AAGTCTTCTG ACGCCTCCCG CCTCTAGTCA CAAGCCGGAC 
GAGTACCTGG GCGATGAGGG CGATGAGACG GGGGCCAGGA GCGGCATCTC CTCCACAACC 
ACATGCTGCA ACACTGCCGC CAGTAACAAG GGCGGCAAGA GCAGCAGCAA CAATTCGGTG 
ACCAGCTGCA GCAGTAGAAG CAATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723926.1
CDS	274..2403
Translation	MGLNAKSVCSTSSTEPNGSIVTTAPSNGEVSSSIVVVVSSSSISSSSDSPIAILPHPDPIPSTSFSSASQRSEEELPGTSAASRTDEMCSCDSQNLAASTAATSNGNKRKRRLSSDSNEDPELGFEPPSAKRQQRLPALYGSEQGNLSSVASSVYTSPVVSVDGQSTQELLSIRSSPAEDLSEAPHSPLPDSPDSPPSPDRGSKQTPVVVRYAAEQVVTSTVVTQKTEDDDLLDDSCEDYSYDEDDEDDVEEEDDDVEIYSSTISPASSGCSQQQAVNGERTPGLPKHQEQIHHPVSDLMINMRTPMSPAVENGLRQCPLPALAWANAADVWRLMCHRDEQDSRLRSISMLEQHPGLQPRMRAILLDWLIEVCEVYKLHRETFYLAVDYLDRYLHVAHKVQKTHLQLIGITCLFVAAKVEEIYPPKIGEFAYVTDGACTERDILNHEKILLQALDWDISPITITGWLGVYMQLNVNNRTPASFSQIGRQKSAEADDAFIYPQFSGFEFVQTSQLLDLCTLDVGMANYSYSVLAAAAISHTFSREMALRCSGLDWQVIQPCARWMEPFFRVISQKAPYLQLNEQNEQVSNKFGLGLICPNIVTDDSHIIQTHTTTMDMYDEVLMAQDAAHAMRARIQASPATALRAPESLLTPPASSHKPDEYLGDEGDETGARSGISSTTTCCNTAASNKGGKSSSNNSVTSCSSRSNP

Target ORF information:

RefSeq Version	NM_165125.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cyclin E, transcript variant E (CycE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165125.3

ORF Insert Sequence:

ATGGGTTTAA ATGCCAAGAG TGTTTGTTCT ACCAGCAGCA CTGAGCCAAA TGGCAGCATA 
GTCACAACCG CCCCCAGCAA CGGAGAAGTG AGCAGCAGCA TAGTCGTCGT CGTCAGCAGC 
AGTAGTATCA GTAGCAGCAG CGATAGCCCA ATCGCTATCC TTCCACATCC AGATCCAATC 
CCATCAACAT CGTTCTCTTC AGCGTCGCAA CGAAGTGAAG AGGAGCTACC AGGAACCTCA 
GCAGCCTCCA GAACCGACGA GATGTGCTCT TGTGATAGCC AGAATCTTGC AGCATCCACA 
GCCGCGACTT CCAATGGCAA TAAACGTAAA CGGCGTTTAA GCAGCGATTC AAACGAGGAC 
CCTGAACTCG GTTTTGAGCC TCCATCAGCT AAGCGCCAGC AGCGACTGCC CGCTTTGTAC 
GGCAGCGAGC AGGGCAATCT GTCATCGGTT GCCTCCTCGG TCTACACCTC GCCCGTTGTC 
TCGGTTGATG GCCAGAGTAC CCAGGAGCTG CTCAGCATAC GTAGCTCACC GGCCGAGGAT 
CTGTCGGAGG CGCCACACAG TCCGCTGCCG GACAGCCCGG ACAGTCCGCC GAGTCCGGAT 
CGTGGAAGCA AGCAGACGCC AGTGGTGGTG CGCTACGCTG CGGAGCAGGT GGTGACCAGT 
ACGGTGGTCA CGCAGAAGAC GGAGGATGAT GACCTTTTGG ACGACAGCTG CGAGGACTAC 
TCGTACGACG AGGATGATGA GGATGACGTT GAGGAGGAGG ACGACGACGT GGAGATCTAC 
TCCTCCACAA TTTCTCCAGC CTCCTCCGGC TGCAGCCAGC AGCAGGCGGT GAATGGAGAG 
CGTACTCCCG GCCTGCCAAA ACATCAGGAG CAGATCCATC ATCCGGTCAG TGACCTGATG 
ATTAACATGC GGACGCCCAT GTCGCCGGCT GTGGAGAACG GTCTGCGCCA GTGCCCACTT 
CCTGCGCTCG CCTGGGCTAA CGCCGCCGAT GTTTGGCGCC TGATGTGTCA CCGGGATGAG 
CAGGACTCGC GTCTGCGCAG CATTTCGATG CTGGAACAGC ATCCCGGACT GCAACCACGT 
ATGCGTGCCA TTCTCTTGGA CTGGTTGATC GAGGTCTGTG AAGTTTACAA GCTGCATCGG 
GAGACCTTCT ACTTGGCCGT CGACTACCTG GATCGCTATT TGCATGTGGC GCATAAGGTG 
CAGAAGACGC ACTTGCAGTT GATCGGCATC ACCTGCCTGT TTGTGGCCGC CAAGGTAGAG 
GAGATTTATC CGCCAAAGAT CGGGGAGTTT GCTTATGTGA CGGATGGCGC CTGCACAGAG 
CGGGACATCC TTAACCACGA GAAGATTCTG CTGCAGGCGC TCGACTGGGA CATCAGCCCC 
ATCACCATTA CCGGCTGGCT GGGCGTCTAT ATGCAACTGA ATGTGAACAA CCGCACTCCG 
GCCTCGTTCT CCCAGATTGG CAGGCAAAAG AGCGCGGAGG CGGACGATGC CTTTATCTAC 
CCGCAATTCT CTGGCTTTGA GTTTGTGCAA ACCTCACAGC TGCTAGATCT GTGCACCCTG 
GACGTGGGCA TGGCCAACTA TTCCTACTCG GTGCTGGCAG CGGCCGCCAT CAGTCATACA 
TTTAGTCGGG AGATGGCTTT ACGCTGTTCT GGTCTGGATT GGCAGGTGAT TCAGCCTTGT 
GCTCGCTGGA TGGAGCCATT CTTCCGAGTG ATCTCCCAGA AGGCCCCCTA CCTGCAGCTG 
AACGAGCAGA ATGAGCAAGT CAGCAACAAA TTTGGCCTGG GACTAATCTG CCCCAATATT 
GTCACCGACG ACTCGCACAT TATCCAAACG CACACAACTA CCATGGATAT GTATGACGAA 
GTGCTTATGG CCCAGGATGC GGCGCATGCC ATGCGCGCTA GGATTCAAGC TTCCCCGGCC 
ACCGCGCTGC GCGCTCCCGA AAGTCTTCTG ACGCCTCCCG CCTCTAGTCA CAAGCCGGAC 
GAGTACCTGG GCGATGAGGG CGATGAGACG GGGGCCAGGA GCGGCATCTC CTCCACAACC 
ACATGCTGCA ACACTGCCGC CAGTAACAAG GGCGGCAAGA GCAGCAGCAA CAATTCGGTG 
ACCAGCTGCA GCAGTAGAAG CAATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CycE ( NM_165124.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723925.1 Drosophila melanogaster cyclin E, transcript variant D (CycE), mRNA.

Price & Availability↑

CloneID	OFa16791
Clone ID Related Accession (Same CDS sequence)	NM_165123.3 , NM_165125.3 , NM_165124.3 , NM_057611.5
Accession Version	NM_165124.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cyclin E, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165124.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTTAA ATGCCAAGAG TGTTTGTTCT ACCAGCAGCA CTGAGCCAAA TGGCAGCATA 
GTCACAACCG CCCCCAGCAA CGGAGAAGTG AGCAGCAGCA TAGTCGTCGT CGTCAGCAGC 
AGTAGTATCA GTAGCAGCAG CGATAGCCCA ATCGCTATCC TTCCACATCC AGATCCAATC 
CCATCAACAT CGTTCTCTTC AGCGTCGCAA CGAAGTGAAG AGGAGCTACC AGGAACCTCA 
GCAGCCTCCA GAACCGACGA GATGTGCTCT TGTGATAGCC AGAATCTTGC AGCATCCACA 
GCCGCGACTT CCAATGGCAA TAAACGTAAA CGGCGTTTAA GCAGCGATTC AAACGAGGAC 
CCTGAACTCG GTTTTGAGCC TCCATCAGCT AAGCGCCAGC AGCGACTGCC CGCTTTGTAC 
GGCAGCGAGC AGGGCAATCT GTCATCGGTT GCCTCCTCGG TCTACACCTC GCCCGTTGTC 
TCGGTTGATG GCCAGAGTAC CCAGGAGCTG CTCAGCATAC GTAGCTCACC GGCCGAGGAT 
CTGTCGGAGG CGCCACACAG TCCGCTGCCG GACAGCCCGG ACAGTCCGCC GAGTCCGGAT 
CGTGGAAGCA AGCAGACGCC AGTGGTGGTG CGCTACGCTG CGGAGCAGGT GGTGACCAGT 
ACGGTGGTCA CGCAGAAGAC GGAGGATGAT GACCTTTTGG ACGACAGCTG CGAGGACTAC 
TCGTACGACG AGGATGATGA GGATGACGTT GAGGAGGAGG ACGACGACGT GGAGATCTAC 
TCCTCCACAA TTTCTCCAGC CTCCTCCGGC TGCAGCCAGC AGCAGGCGGT GAATGGAGAG 
CGTACTCCCG GCCTGCCAAA ACATCAGGAG CAGATCCATC ATCCGGTCAG TGACCTGATG 
ATTAACATGC GGACGCCCAT GTCGCCGGCT GTGGAGAACG GTCTGCGCCA GTGCCCACTT 
CCTGCGCTCG CCTGGGCTAA CGCCGCCGAT GTTTGGCGCC TGATGTGTCA CCGGGATGAG 
CAGGACTCGC GTCTGCGCAG CATTTCGATG CTGGAACAGC ATCCCGGACT GCAACCACGT 
ATGCGTGCCA TTCTCTTGGA CTGGTTGATC GAGGTCTGTG AAGTTTACAA GCTGCATCGG 
GAGACCTTCT ACTTGGCCGT CGACTACCTG GATCGCTATT TGCATGTGGC GCATAAGGTG 
CAGAAGACGC ACTTGCAGTT GATCGGCATC ACCTGCCTGT TTGTGGCCGC CAAGGTAGAG 
GAGATTTATC CGCCAAAGAT CGGGGAGTTT GCTTATGTGA CGGATGGCGC CTGCACAGAG 
CGGGACATCC TTAACCACGA GAAGATTCTG CTGCAGGCGC TCGACTGGGA CATCAGCCCC 
ATCACCATTA CCGGCTGGCT GGGCGTCTAT ATGCAACTGA ATGTGAACAA CCGCACTCCG 
GCCTCGTTCT CCCAGATTGG CAGGCAAAAG AGCGCGGAGG CGGACGATGC CTTTATCTAC 
CCGCAATTCT CTGGCTTTGA GTTTGTGCAA ACCTCACAGC TGCTAGATCT GTGCACCCTG 
GACGTGGGCA TGGCCAACTA TTCCTACTCG GTGCTGGCAG CGGCCGCCAT CAGTCATACA 
TTTAGTCGGG AGATGGCTTT ACGCTGTTCT GGTCTGGATT GGCAGGTGAT TCAGCCTTGT 
GCTCGCTGGA TGGAGCCATT CTTCCGAGTG ATCTCCCAGA AGGCCCCCTA CCTGCAGCTG 
AACGAGCAGA ATGAGCAAGT CAGCAACAAA TTTGGCCTGG GACTAATCTG CCCCAATATT 
GTCACCGACG ACTCGCACAT TATCCAAACG CACACAACTA CCATGGATAT GTATGACGAA 
GTGCTTATGG CCCAGGATGC GGCGCATGCC ATGCGCGCTA GGATTCAAGC TTCCCCGGCC 
ACCGCGCTGC GCGCTCCCGA AAGTCTTCTG ACGCCTCCCG CCTCTAGTCA CAAGCCGGAC 
GAGTACCTGG GCGATGAGGG CGATGAGACG GGGGCCAGGA GCGGCATCTC CTCCACAACC 
ACATGCTGCA ACACTGCCGC CAGTAACAAG GGCGGCAAGA GCAGCAGCAA CAATTCGGTG 
ACCAGCTGCA GCAGTAGAAG CAATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723925.1
CDS	517..2646
Translation	MGLNAKSVCSTSSTEPNGSIVTTAPSNGEVSSSIVVVVSSSSISSSSDSPIAILPHPDPIPSTSFSSASQRSEEELPGTSAASRTDEMCSCDSQNLAASTAATSNGNKRKRRLSSDSNEDPELGFEPPSAKRQQRLPALYGSEQGNLSSVASSVYTSPVVSVDGQSTQELLSIRSSPAEDLSEAPHSPLPDSPDSPPSPDRGSKQTPVVVRYAAEQVVTSTVVTQKTEDDDLLDDSCEDYSYDEDDEDDVEEEDDDVEIYSSTISPASSGCSQQQAVNGERTPGLPKHQEQIHHPVSDLMINMRTPMSPAVENGLRQCPLPALAWANAADVWRLMCHRDEQDSRLRSISMLEQHPGLQPRMRAILLDWLIEVCEVYKLHRETFYLAVDYLDRYLHVAHKVQKTHLQLIGITCLFVAAKVEEIYPPKIGEFAYVTDGACTERDILNHEKILLQALDWDISPITITGWLGVYMQLNVNNRTPASFSQIGRQKSAEADDAFIYPQFSGFEFVQTSQLLDLCTLDVGMANYSYSVLAAAAISHTFSREMALRCSGLDWQVIQPCARWMEPFFRVISQKAPYLQLNEQNEQVSNKFGLGLICPNIVTDDSHIIQTHTTTMDMYDEVLMAQDAAHAMRARIQASPATALRAPESLLTPPASSHKPDEYLGDEGDETGARSGISSTTTCCNTAASNKGGKSSSNNSVTSCSSRSNP

Target ORF information:

RefSeq Version	NM_165124.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cyclin E, transcript variant D (CycE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165124.3

ORF Insert Sequence:

ATGGGTTTAA ATGCCAAGAG TGTTTGTTCT ACCAGCAGCA CTGAGCCAAA TGGCAGCATA 
GTCACAACCG CCCCCAGCAA CGGAGAAGTG AGCAGCAGCA TAGTCGTCGT CGTCAGCAGC 
AGTAGTATCA GTAGCAGCAG CGATAGCCCA ATCGCTATCC TTCCACATCC AGATCCAATC 
CCATCAACAT CGTTCTCTTC AGCGTCGCAA CGAAGTGAAG AGGAGCTACC AGGAACCTCA 
GCAGCCTCCA GAACCGACGA GATGTGCTCT TGTGATAGCC AGAATCTTGC AGCATCCACA 
GCCGCGACTT CCAATGGCAA TAAACGTAAA CGGCGTTTAA GCAGCGATTC AAACGAGGAC 
CCTGAACTCG GTTTTGAGCC TCCATCAGCT AAGCGCCAGC AGCGACTGCC CGCTTTGTAC 
GGCAGCGAGC AGGGCAATCT GTCATCGGTT GCCTCCTCGG TCTACACCTC GCCCGTTGTC 
TCGGTTGATG GCCAGAGTAC CCAGGAGCTG CTCAGCATAC GTAGCTCACC GGCCGAGGAT 
CTGTCGGAGG CGCCACACAG TCCGCTGCCG GACAGCCCGG ACAGTCCGCC GAGTCCGGAT 
CGTGGAAGCA AGCAGACGCC AGTGGTGGTG CGCTACGCTG CGGAGCAGGT GGTGACCAGT 
ACGGTGGTCA CGCAGAAGAC GGAGGATGAT GACCTTTTGG ACGACAGCTG CGAGGACTAC 
TCGTACGACG AGGATGATGA GGATGACGTT GAGGAGGAGG ACGACGACGT GGAGATCTAC 
TCCTCCACAA TTTCTCCAGC CTCCTCCGGC TGCAGCCAGC AGCAGGCGGT GAATGGAGAG 
CGTACTCCCG GCCTGCCAAA ACATCAGGAG CAGATCCATC ATCCGGTCAG TGACCTGATG 
ATTAACATGC GGACGCCCAT GTCGCCGGCT GTGGAGAACG GTCTGCGCCA GTGCCCACTT 
CCTGCGCTCG CCTGGGCTAA CGCCGCCGAT GTTTGGCGCC TGATGTGTCA CCGGGATGAG 
CAGGACTCGC GTCTGCGCAG CATTTCGATG CTGGAACAGC ATCCCGGACT GCAACCACGT 
ATGCGTGCCA TTCTCTTGGA CTGGTTGATC GAGGTCTGTG AAGTTTACAA GCTGCATCGG 
GAGACCTTCT ACTTGGCCGT CGACTACCTG GATCGCTATT TGCATGTGGC GCATAAGGTG 
CAGAAGACGC ACTTGCAGTT GATCGGCATC ACCTGCCTGT TTGTGGCCGC CAAGGTAGAG 
GAGATTTATC CGCCAAAGAT CGGGGAGTTT GCTTATGTGA CGGATGGCGC CTGCACAGAG 
CGGGACATCC TTAACCACGA GAAGATTCTG CTGCAGGCGC TCGACTGGGA CATCAGCCCC 
ATCACCATTA CCGGCTGGCT GGGCGTCTAT ATGCAACTGA ATGTGAACAA CCGCACTCCG 
GCCTCGTTCT CCCAGATTGG CAGGCAAAAG AGCGCGGAGG CGGACGATGC CTTTATCTAC 
CCGCAATTCT CTGGCTTTGA GTTTGTGCAA ACCTCACAGC TGCTAGATCT GTGCACCCTG 
GACGTGGGCA TGGCCAACTA TTCCTACTCG GTGCTGGCAG CGGCCGCCAT CAGTCATACA 
TTTAGTCGGG AGATGGCTTT ACGCTGTTCT GGTCTGGATT GGCAGGTGAT TCAGCCTTGT 
GCTCGCTGGA TGGAGCCATT CTTCCGAGTG ATCTCCCAGA AGGCCCCCTA CCTGCAGCTG 
AACGAGCAGA ATGAGCAAGT CAGCAACAAA TTTGGCCTGG GACTAATCTG CCCCAATATT 
GTCACCGACG ACTCGCACAT TATCCAAACG CACACAACTA CCATGGATAT GTATGACGAA 
GTGCTTATGG CCCAGGATGC GGCGCATGCC ATGCGCGCTA GGATTCAAGC TTCCCCGGCC 
ACCGCGCTGC GCGCTCCCGA AAGTCTTCTG ACGCCTCCCG CCTCTAGTCA CAAGCCGGAC 
GAGTACCTGG GCGATGAGGG CGATGAGACG GGGGCCAGGA GCGGCATCTC CTCCACAACC 
ACATGCTGCA ACACTGCCGC CAGTAACAAG GGCGGCAAGA GCAGCAGCAA CAATTCGGTG 
ACCAGCTGCA GCAGTAGAAG CAATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CycE ( NM_057611.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476959.1 Drosophila melanogaster cyclin E, transcript variant A (CycE), mRNA.

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CloneID	OFa16791
Clone ID Related Accession (Same CDS sequence)	NM_165123.3 , NM_165125.3 , NM_165124.3 , NM_057611.5
Accession Version	NM_057611.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cyclin E, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057611.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTTAA ATGCCAAGAG TGTTTGTTCT ACCAGCAGCA CTGAGCCAAA TGGCAGCATA 
GTCACAACCG CCCCCAGCAA CGGAGAAGTG AGCAGCAGCA TAGTCGTCGT CGTCAGCAGC 
AGTAGTATCA GTAGCAGCAG CGATAGCCCA ATCGCTATCC TTCCACATCC AGATCCAATC 
CCATCAACAT CGTTCTCTTC AGCGTCGCAA CGAAGTGAAG AGGAGCTACC AGGAACCTCA 
GCAGCCTCCA GAACCGACGA GATGTGCTCT TGTGATAGCC AGAATCTTGC AGCATCCACA 
GCCGCGACTT CCAATGGCAA TAAACGTAAA CGGCGTTTAA GCAGCGATTC AAACGAGGAC 
CCTGAACTCG GTTTTGAGCC TCCATCAGCT AAGCGCCAGC AGCGACTGCC CGCTTTGTAC 
GGCAGCGAGC AGGGCAATCT GTCATCGGTT GCCTCCTCGG TCTACACCTC GCCCGTTGTC 
TCGGTTGATG GCCAGAGTAC CCAGGAGCTG CTCAGCATAC GTAGCTCACC GGCCGAGGAT 
CTGTCGGAGG CGCCACACAG TCCGCTGCCG GACAGCCCGG ACAGTCCGCC GAGTCCGGAT 
CGTGGAAGCA AGCAGACGCC AGTGGTGGTG CGCTACGCTG CGGAGCAGGT GGTGACCAGT 
ACGGTGGTCA CGCAGAAGAC GGAGGATGAT GACCTTTTGG ACGACAGCTG CGAGGACTAC 
TCGTACGACG AGGATGATGA GGATGACGTT GAGGAGGAGG ACGACGACGT GGAGATCTAC 
TCCTCCACAA TTTCTCCAGC CTCCTCCGGC TGCAGCCAGC AGCAGGCGGT GAATGGAGAG 
CGTACTCCCG GCCTGCCAAA ACATCAGGAG CAGATCCATC ATCCGGTCAG TGACCTGATG 
ATTAACATGC GGACGCCCAT GTCGCCGGCT GTGGAGAACG GTCTGCGCCA GTGCCCACTT 
CCTGCGCTCG CCTGGGCTAA CGCCGCCGAT GTTTGGCGCC TGATGTGTCA CCGGGATGAG 
CAGGACTCGC GTCTGCGCAG CATTTCGATG CTGGAACAGC ATCCCGGACT GCAACCACGT 
ATGCGTGCCA TTCTCTTGGA CTGGTTGATC GAGGTCTGTG AAGTTTACAA GCTGCATCGG 
GAGACCTTCT ACTTGGCCGT CGACTACCTG GATCGCTATT TGCATGTGGC GCATAAGGTG 
CAGAAGACGC ACTTGCAGTT GATCGGCATC ACCTGCCTGT TTGTGGCCGC CAAGGTAGAG 
GAGATTTATC CGCCAAAGAT CGGGGAGTTT GCTTATGTGA CGGATGGCGC CTGCACAGAG 
CGGGACATCC TTAACCACGA GAAGATTCTG CTGCAGGCGC TCGACTGGGA CATCAGCCCC 
ATCACCATTA CCGGCTGGCT GGGCGTCTAT ATGCAACTGA ATGTGAACAA CCGCACTCCG 
GCCTCGTTCT CCCAGATTGG CAGGCAAAAG AGCGCGGAGG CGGACGATGC CTTTATCTAC 
CCGCAATTCT CTGGCTTTGA GTTTGTGCAA ACCTCACAGC TGCTAGATCT GTGCACCCTG 
GACGTGGGCA TGGCCAACTA TTCCTACTCG GTGCTGGCAG CGGCCGCCAT CAGTCATACA 
TTTAGTCGGG AGATGGCTTT ACGCTGTTCT GGTCTGGATT GGCAGGTGAT TCAGCCTTGT 
GCTCGCTGGA TGGAGCCATT CTTCCGAGTG ATCTCCCAGA AGGCCCCCTA CCTGCAGCTG 
AACGAGCAGA ATGAGCAAGT CAGCAACAAA TTTGGCCTGG GACTAATCTG CCCCAATATT 
GTCACCGACG ACTCGCACAT TATCCAAACG CACACAACTA CCATGGATAT GTATGACGAA 
GTGCTTATGG CCCAGGATGC GGCGCATGCC ATGCGCGCTA GGATTCAAGC TTCCCCGGCC 
ACCGCGCTGC GCGCTCCCGA AAGTCTTCTG ACGCCTCCCG CCTCTAGTCA CAAGCCGGAC 
GAGTACCTGG GCGATGAGGG CGATGAGACG GGGGCCAGGA GCGGCATCTC CTCCACAACC 
ACATGCTGCA ACACTGCCGC CAGTAACAAG GGCGGCAAGA GCAGCAGCAA CAATTCGGTG 
ACCAGCTGCA GCAGTAGAAG CAATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476959.1
CDS	427..2556
Translation	MGLNAKSVCSTSSTEPNGSIVTTAPSNGEVSSSIVVVVSSSSISSSSDSPIAILPHPDPIPSTSFSSASQRSEEELPGTSAASRTDEMCSCDSQNLAASTAATSNGNKRKRRLSSDSNEDPELGFEPPSAKRQQRLPALYGSEQGNLSSVASSVYTSPVVSVDGQSTQELLSIRSSPAEDLSEAPHSPLPDSPDSPPSPDRGSKQTPVVVRYAAEQVVTSTVVTQKTEDDDLLDDSCEDYSYDEDDEDDVEEEDDDVEIYSSTISPASSGCSQQQAVNGERTPGLPKHQEQIHHPVSDLMINMRTPMSPAVENGLRQCPLPALAWANAADVWRLMCHRDEQDSRLRSISMLEQHPGLQPRMRAILLDWLIEVCEVYKLHRETFYLAVDYLDRYLHVAHKVQKTHLQLIGITCLFVAAKVEEIYPPKIGEFAYVTDGACTERDILNHEKILLQALDWDISPITITGWLGVYMQLNVNNRTPASFSQIGRQKSAEADDAFIYPQFSGFEFVQTSQLLDLCTLDVGMANYSYSVLAAAAISHTFSREMALRCSGLDWQVIQPCARWMEPFFRVISQKAPYLQLNEQNEQVSNKFGLGLICPNIVTDDSHIIQTHTTTMDMYDEVLMAQDAAHAMRARIQASPATALRAPESLLTPPASSHKPDEYLGDEGDETGARSGISSTTTCCNTAASNKGGKSSSNNSVTSCSSRSNP

Target ORF information:

RefSeq Version	NM_057611.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cyclin E, transcript variant A (CycE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057611.5

ORF Insert Sequence:

ATGGGTTTAA ATGCCAAGAG TGTTTGTTCT ACCAGCAGCA CTGAGCCAAA TGGCAGCATA 
GTCACAACCG CCCCCAGCAA CGGAGAAGTG AGCAGCAGCA TAGTCGTCGT CGTCAGCAGC 
AGTAGTATCA GTAGCAGCAG CGATAGCCCA ATCGCTATCC TTCCACATCC AGATCCAATC 
CCATCAACAT CGTTCTCTTC AGCGTCGCAA CGAAGTGAAG AGGAGCTACC AGGAACCTCA 
GCAGCCTCCA GAACCGACGA GATGTGCTCT TGTGATAGCC AGAATCTTGC AGCATCCACA 
GCCGCGACTT CCAATGGCAA TAAACGTAAA CGGCGTTTAA GCAGCGATTC AAACGAGGAC 
CCTGAACTCG GTTTTGAGCC TCCATCAGCT AAGCGCCAGC AGCGACTGCC CGCTTTGTAC 
GGCAGCGAGC AGGGCAATCT GTCATCGGTT GCCTCCTCGG TCTACACCTC GCCCGTTGTC 
TCGGTTGATG GCCAGAGTAC CCAGGAGCTG CTCAGCATAC GTAGCTCACC GGCCGAGGAT 
CTGTCGGAGG CGCCACACAG TCCGCTGCCG GACAGCCCGG ACAGTCCGCC GAGTCCGGAT 
CGTGGAAGCA AGCAGACGCC AGTGGTGGTG CGCTACGCTG CGGAGCAGGT GGTGACCAGT 
ACGGTGGTCA CGCAGAAGAC GGAGGATGAT GACCTTTTGG ACGACAGCTG CGAGGACTAC 
TCGTACGACG AGGATGATGA GGATGACGTT GAGGAGGAGG ACGACGACGT GGAGATCTAC 
TCCTCCACAA TTTCTCCAGC CTCCTCCGGC TGCAGCCAGC AGCAGGCGGT GAATGGAGAG 
CGTACTCCCG GCCTGCCAAA ACATCAGGAG CAGATCCATC ATCCGGTCAG TGACCTGATG 
ATTAACATGC GGACGCCCAT GTCGCCGGCT GTGGAGAACG GTCTGCGCCA GTGCCCACTT 
CCTGCGCTCG CCTGGGCTAA CGCCGCCGAT GTTTGGCGCC TGATGTGTCA CCGGGATGAG 
CAGGACTCGC GTCTGCGCAG CATTTCGATG CTGGAACAGC ATCCCGGACT GCAACCACGT 
ATGCGTGCCA TTCTCTTGGA CTGGTTGATC GAGGTCTGTG AAGTTTACAA GCTGCATCGG 
GAGACCTTCT ACTTGGCCGT CGACTACCTG GATCGCTATT TGCATGTGGC GCATAAGGTG 
CAGAAGACGC ACTTGCAGTT GATCGGCATC ACCTGCCTGT TTGTGGCCGC CAAGGTAGAG 
GAGATTTATC CGCCAAAGAT CGGGGAGTTT GCTTATGTGA CGGATGGCGC CTGCACAGAG 
CGGGACATCC TTAACCACGA GAAGATTCTG CTGCAGGCGC TCGACTGGGA CATCAGCCCC 
ATCACCATTA CCGGCTGGCT GGGCGTCTAT ATGCAACTGA ATGTGAACAA CCGCACTCCG 
GCCTCGTTCT CCCAGATTGG CAGGCAAAAG AGCGCGGAGG CGGACGATGC CTTTATCTAC 
CCGCAATTCT CTGGCTTTGA GTTTGTGCAA ACCTCACAGC TGCTAGATCT GTGCACCCTG 
GACGTGGGCA TGGCCAACTA TTCCTACTCG GTGCTGGCAG CGGCCGCCAT CAGTCATACA 
TTTAGTCGGG AGATGGCTTT ACGCTGTTCT GGTCTGGATT GGCAGGTGAT TCAGCCTTGT 
GCTCGCTGGA TGGAGCCATT CTTCCGAGTG ATCTCCCAGA AGGCCCCCTA CCTGCAGCTG 
AACGAGCAGA ATGAGCAAGT CAGCAACAAA TTTGGCCTGG GACTAATCTG CCCCAATATT 
GTCACCGACG ACTCGCACAT TATCCAAACG CACACAACTA CCATGGATAT GTATGACGAA 
GTGCTTATGG CCCAGGATGC GGCGCATGCC ATGCGCGCTA GGATTCAAGC TTCCCCGGCC 
ACCGCGCTGC GCGCTCCCGA AAGTCTTCTG ACGCCTCCCG CCTCTAGTCA CAAGCCGGAC 
GAGTACCTGG GCGATGAGGG CGATGAGACG GGGGCCAGGA GCGGCATCTC CTCCACAACC 
ACATGCTGCA ACACTGCCGC CAGTAACAAG GGCGGCAAGA GCAGCAGCAA CAATTCGGTG 
ACCAGCTGCA GCAGTAGAAG CAATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CycE cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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