Cul3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cul3
Entrez Gene ID	34896
Full Name	Cullin 3
Synonyms	BG:DS07851.2,CG11861,CG31829,CG42616,CUL-3,CUL3,Cul-3,Cullin3,DMcul-3,Dmel\CG42616,Dmel_CG11861,Dmel_CG31829,br34,cul-3,cul3,dCul-3,dCul3,gft,gft/dCul-3,guftagu,l(2)06430,l(2)35Cd,l(2)br34,l35Cd,mds
General protein information	Preferred Names Cullin 3 Names CG42616 gene product from transcript CG42616-RD CG42616-PC CG42616-PD CG42616-PE CG42616-PF Cul3-PC Cul3-PD Cul3-PE Cul3-PF Cullin3 cullin cullin 3 cullin-3 guftagu medusa
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35C5-35C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_165110.2	NP_723907.1	cullin 3, isoform E
NM_165111.3	NP_723908.2	cullin 3, isoform D
NM_078849.3	NP_523573.1	cullin 3, isoform C
NM_165112.2	NP_723909.2	cullin 3, isoform F

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	cul3b	NP_001177414.1
Oryza sativa (rice)	Os08g0170900	NP_001061094.1
Xenopus tropicalis (tropical clawed frog)	cul3	NP_001015975.2
	CUL3	XP_001109745.1
Homo sapiens (human)	CUL3	NP_003581.1
Canis lupus familiaris (dog)	CUL3	XP_534586.3
Oryza sativa (rice)	Os04g0643000	NP_001054047.1
Mus musculus (house mouse)	Cul3	NP_057925.1
Drosophila melanogaster (fruit fly)	Cul-3	NP_723907.1
Caenorhabditis elegans (roundworm)	cul-3	NP_503151.1
Pan troglodytes (chimpanzee)	CUL3	XP_001167528.1
Bos taurus (cattle)	CUL3	NP_001179735.1
Rattus norvegicus (Norway rat)	Cul3	NP_001100393.1
Gallus gallus (chicken)	CUL3	XP_422620.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008105	XP_317352.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CRL3(GCL) promotes primordial germ cell fate by mediating degradation of Torso, a receptor tyrosine kinase (RTK) and major determinant of somatic cell fate.
Title: GCL and CUL3 Control the Switch between Cell Lineages by Mediating Localized Degradation of an RTK.

Data show that insomniac protein (INC) recognize its potential partners Cul3 and dGRASP.
Title: Proteins involved in sleep homeostasis: Biophysical characterization of INC and its partners.

A Cul-3-BTB ubiquitylation pathway regulates junctional levels and asymmetry of core planar polarity proteins.
Title: A Cul-3-BTB ubiquitylation pathway regulates junctional levels and asymmetry of core planar polarity proteins.

Deregulation of CUL-3 in the clock neurons induces behavioral arrhythmicity.
Title: CULLIN-3 controls TIMELESS oscillations in the Drosophila circadian clock.

these results establish inc and Cul3 as important new players in setting the sleep homeostat and a dopaminergic arousal pathway in Drosophila.
Title: Cul3 and the BTB adaptor insomniac are key regulators of sleep homeostasis and a dopamine arousal pathway in Drosophila.

The following Cul3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cul3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15909	NM_078849.3 Latest version!	Drosophila melanogaster cullin 3, transcript variant C (Cul3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15909	NM_165111.3 Latest version!	Drosophila melanogaster cullin 3, transcript variant D (Cul3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15909	NM_165110.2 Latest version!	Drosophila melanogaster cullin 3, transcript variant E (Cul3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15910	NM_165112.2 Latest version!	Drosophila melanogaster cullin 3, transcript variant F (Cul3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cul3 ( NM_165110.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723907.1 Drosophila melanogaster cullin 3, transcript variant E (Cul3), mRNA.

Price & Availability↑

CloneID	OFa15909
Clone ID Related Accession (Same CDS sequence)	NM_165110.2 , NM_165111.3 , NM_078849.3
Accession Version	NM_165110.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cullin 3, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_165110.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTGC GGGGAAATCC TCCCAAGAAG GAGGGCAAAA TGCGCATTCG GGCCTTTCCG 
GCCTCCATGG ACGAGAAGTA CGTGGAGACC ATTTGGGCCA GCTTAAAGAA TGCTATCCAG 
GAGATACAGA AAAAGAACAA CTCCGGACTA TCGTTTGAAC AACTCTACCG GAATGCGTAC 
AACATGGTGC TGCATAAGCA CGGCAATAGA CTGTATTACG GTCTGAGGGA GGTCGTCTCA 
GAACACCTGG AGCACAAGGT GCGGGCGGAT GTGCTGGAGG CTCTGCACAG CAATTTCCTA 
CCCAAGTTAA ACCAGGCCTG GACAGACCAC CAGACATCTA TGGTGATGAT CCGCGACATA 
CTCATGTACA TGGATAGAGT TTATGTGCAG CAGCGCGAGG TAGACAATGT GTACAATTTG 
GGATTAATTT TGTTCAGGGA TCAGGTGGTT CGCTATTCTG AGATTCAAAA GGCCCTGCGA 
GAGAAGTTGC TGGGCATGGT GATGGAGGAA CGTCATGGCG AAGCCATTAA CCATCTGGCT 
ATCAAGAATG CTTGCAGTAT GCTGATCACC CTGGGGATTA ACTCGCGCAC TGTCTACGAA 
GAGGACTTTG AAAAGCCCTT CCTTGCGCAA TCGGCGGCGT TTTACAAATT CGAGTCGCAG 
AACTTTCTTG CCGAGAACAA CGCTGGCGTT TACATCAAGA AAGTGGAAGC GCGCATCACG 
GAGGAATCTT CCCGGGCAGC GCTGTATCTC GATAAGGATA CGGAGCCCCG CATTGTGCGC 
GTGGTCGAAG AAGAGCTGAT CAAAAAGCAC ATGCGACCCA TTGTTGAAAT GGAGAACTCG 
GGCGTGGTGT ACATGATCAA GAACTCAAAG ACCGAGGATC TGGCCTGCAC ATATAAGCTT 
TTCTCGCGCC TGAAGGAGGA GGGTCTCAAG GTGATAGCGG ACACAATGTC GGCATATCTG 
CGAGAGCAAG GACGCATGCT GGTTAAGGAA GAAGAAAACG GCAACACGAA TCCCATAACA 
TTCGTGCAGA ACTTGCTGGA TCTCAAGGAC CGCTTCGACC AGTTTCTGGT GCACTCGTTC 
GCCAACGATC GCATATTCAA AAACGTCATC TCATCCGATT TTGAACATTT CTTGAACCTA 
AATAACAAAT CCCCGGAGTA TCTATCGCTA TTCATCGATG ACAAACTGAA AAAGGGTGGC 
AAGGGAATGA GCGAACAGGA AATTGAGTCC ATCTTGGATA AGACAATGGT TCTCTTCCGT 
TTCCTATTGG AGAAAGATGT CTTTGAGCGC TATTACAAGA CGCATTTAGC CAAGAGATTG 
CTGCTGAACA AATCAGTCTC TGATGATTTC GAGAAGAATA TGATATCAAA ACTAAAGACT 
GAATGCGGCT GTCAATTCAC CTCGAAGCTA GAGGGCATGT TTAAAGATAT GTCAGTCTCC 
AATACGATCA TGGATGAGTT TAAGAACTTT GTAAATAATA ACAATTTATC CCTTGGCGGT 
GTGGAGCTAA CGGTACGCAT ACTAACCACT GGTTTTTGGC CCACACAGAC AGCAACTCCG 
AACTGCAATA TACCCGCCGC TCCGCGTGAA GCCTTTGACA TTTTCAAGAA CTTCTATCTA 
AATAAGCACT CAGGACGTCA ATTGACGTTA CAGCCACAAA TGGGAACCGC CTATATCAAC 
GCTGTGTTTT ATGGCCGCAA GGCAGTTGAA AGTGAAAAGG ATAAAGATGC ACCCAGCTCC 
AGTTCAAGCG GCTGTGGGGT GCCCACCACC ACACGCAAGC ACATATTGCA AGTGTCCACG 
TACCAGATGT GTGTGCTACT GCTGTTTAAT AACCGCGACG TGCTCACCTA CGATGACATA 
CACCAGGAGA CCGATATACC GGAACGGGAG CTCGTCCGAG CACTACAATC GTTGTCCATG 
GGCAAACCCG CTCAGCGTTT GTTAGTACGC AATTCTAAAA CGAAAACTAA GGACATTGAG 
CCCACGGATG AGTTTTACGT AAACGACGCC TTCAACTCCA AGTTCCACAG GGTGAAGATA 
CAAACGGTAG CCGCCAAGGG AGAATCAGAG CCAGAGCGTA AGGAAACGCG CGGAAAGGTC 
GACGAGGATC GTAAGCACGA GATCGAAGCG GCTATTGTGC GCATAATGAA GGCCCGCAAG 
CGTCTGGCTC ACAACTTGCT AGTATCAGAT GTGACGTCGC AGCTAAAGTC GCGTTTCTTG 
CCCTCGCCCG TGTTTATCAA AAAGCGCATT GAGGGCCTAA TCGAGCGCGA GTATCTGCAA 
CGATCGCCGG AAGATCGCAA AGTGTACAAC TACTTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723907.1
CDS	606..2927
Translation	MNLRGNPPKKEGKMRIRAFPASMDEKYVETIWASLKNAIQEIQKKNNSGLSFEQLYRNAYNMVLHKHGNRLYYGLREVVSEHLEHKVRADVLEALHSNFLPKLNQAWTDHQTSMVMIRDILMYMDRVYVQQREVDNVYNLGLILFRDQVVRYSEIQKALREKLLGMVMEERHGEAINHLAIKNACSMLITLGINSRTVYEEDFEKPFLAQSAAFYKFESQNFLAENNAGVYIKKVEARITEESSRAALYLDKDTEPRIVRVVEEELIKKHMRPIVEMENSGVVYMIKNSKTEDLACTYKLFSRLKEEGLKVIADTMSAYLREQGRMLVKEEENGNTNPITFVQNLLDLKDRFDQFLVHSFANDRIFKNVISSDFEHFLNLNNKSPEYLSLFIDDKLKKGGKGMSEQEIESILDKTMVLFRFLLEKDVFERYYKTHLAKRLLLNKSVSDDFEKNMISKLKTECGCQFTSKLEGMFKDMSVSNTIMDEFKNFVNNNNLSLGGVELTVRILTTGFWPTQTATPNCNIPAAPREAFDIFKNFYLNKHSGRQLTLQPQMGTAYINAVFYGRKAVESEKDKDAPSSSSSGCGVPTTTRKHILQVSTYQMCVLLLFNNRDVLTYDDIHQETDIPERELVRALQSLSMGKPAQRLLVRNSKTKTKDIEPTDEFYVNDAFNSKFHRVKIQTVAAKGESEPERKETRGKVDEDRKHEIEAAIVRIMKARKRLAHNLLVSDVTSQLKSRFLPSPVFIKKRIEGLIEREYLQRSPEDRKVYNYLA

Target ORF information:

RefSeq Version	NM_165110.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cullin 3, transcript variant E (Cul3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165110.2

ORF Insert Sequence:

ATGAATCTGC GGGGAAATCC TCCCAAGAAG GAGGGCAAAA TGCGCATTCG GGCCTTTCCG 
GCCTCCATGG ACGAGAAGTA CGTGGAGACC ATTTGGGCCA GCTTAAAGAA TGCTATCCAG 
GAGATACAGA AAAAGAACAA CTCCGGACTA TCGTTTGAAC AACTCTACCG GAATGCGTAC 
AACATGGTGC TGCATAAGCA CGGCAATAGA CTGTATTACG GTCTGAGGGA GGTCGTCTCA 
GAACACCTGG AGCACAAGGT GCGGGCGGAT GTGCTGGAGG CTCTGCACAG CAATTTCCTA 
CCCAAGTTAA ACCAGGCCTG GACAGACCAC CAGACATCTA TGGTGATGAT CCGCGACATA 
CTCATGTACA TGGATAGAGT TTATGTGCAG CAGCGCGAGG TAGACAATGT GTACAATTTG 
GGATTAATTT TGTTCAGGGA TCAGGTGGTT CGCTATTCTG AGATTCAAAA GGCCCTGCGA 
GAGAAGTTGC TGGGCATGGT GATGGAGGAA CGTCATGGCG AAGCCATTAA CCATCTGGCT 
ATCAAGAATG CTTGCAGTAT GCTGATCACC CTGGGGATTA ACTCGCGCAC TGTCTACGAA 
GAGGACTTTG AAAAGCCCTT CCTTGCGCAA TCGGCGGCGT TTTACAAATT CGAGTCGCAG 
AACTTTCTTG CCGAGAACAA CGCTGGCGTT TACATCAAGA AAGTGGAAGC GCGCATCACG 
GAGGAATCTT CCCGGGCAGC GCTGTATCTC GATAAGGATA CGGAGCCCCG CATTGTGCGC 
GTGGTCGAAG AAGAGCTGAT CAAAAAGCAC ATGCGACCCA TTGTTGAAAT GGAGAACTCG 
GGCGTGGTGT ACATGATCAA GAACTCAAAG ACCGAGGATC TGGCCTGCAC ATATAAGCTT 
TTCTCGCGCC TGAAGGAGGA GGGTCTCAAG GTGATAGCGG ACACAATGTC GGCATATCTG 
CGAGAGCAAG GACGCATGCT GGTTAAGGAA GAAGAAAACG GCAACACGAA TCCCATAACA 
TTCGTGCAGA ACTTGCTGGA TCTCAAGGAC CGCTTCGACC AGTTTCTGGT GCACTCGTTC 
GCCAACGATC GCATATTCAA AAACGTCATC TCATCCGATT TTGAACATTT CTTGAACCTA 
AATAACAAAT CCCCGGAGTA TCTATCGCTA TTCATCGATG ACAAACTGAA AAAGGGTGGC 
AAGGGAATGA GCGAACAGGA AATTGAGTCC ATCTTGGATA AGACAATGGT TCTCTTCCGT 
TTCCTATTGG AGAAAGATGT CTTTGAGCGC TATTACAAGA CGCATTTAGC CAAGAGATTG 
CTGCTGAACA AATCAGTCTC TGATGATTTC GAGAAGAATA TGATATCAAA ACTAAAGACT 
GAATGCGGCT GTCAATTCAC CTCGAAGCTA GAGGGCATGT TTAAAGATAT GTCAGTCTCC 
AATACGATCA TGGATGAGTT TAAGAACTTT GTAAATAATA ACAATTTATC CCTTGGCGGT 
GTGGAGCTAA CGGTACGCAT ACTAACCACT GGTTTTTGGC CCACACAGAC AGCAACTCCG 
AACTGCAATA TACCCGCCGC TCCGCGTGAA GCCTTTGACA TTTTCAAGAA CTTCTATCTA 
AATAAGCACT CAGGACGTCA ATTGACGTTA CAGCCACAAA TGGGAACCGC CTATATCAAC 
GCTGTGTTTT ATGGCCGCAA GGCAGTTGAA AGTGAAAAGG ATAAAGATGC ACCCAGCTCC 
AGTTCAAGCG GCTGTGGGGT GCCCACCACC ACACGCAAGC ACATATTGCA AGTGTCCACG 
TACCAGATGT GTGTGCTACT GCTGTTTAAT AACCGCGACG TGCTCACCTA CGATGACATA 
CACCAGGAGA CCGATATACC GGAACGGGAG CTCGTCCGAG CACTACAATC GTTGTCCATG 
GGCAAACCCG CTCAGCGTTT GTTAGTACGC AATTCTAAAA CGAAAACTAA GGACATTGAG 
CCCACGGATG AGTTTTACGT AAACGACGCC TTCAACTCCA AGTTCCACAG GGTGAAGATA 
CAAACGGTAG CCGCCAAGGG AGAATCAGAG CCAGAGCGTA AGGAAACGCG CGGAAAGGTC 
GACGAGGATC GTAAGCACGA GATCGAAGCG GCTATTGTGC GCATAATGAA GGCCCGCAAG 
CGTCTGGCTC ACAACTTGCT AGTATCAGAT GTGACGTCGC AGCTAAAGTC GCGTTTCTTG 
CCCTCGCCCG TGTTTATCAA AAAGCGCATT GAGGGCCTAA TCGAGCGCGA GTATCTGCAA 
CGATCGCCGG AAGATCGCAA AGTGTACAAC TACTTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul3 ( NM_165111.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723908.2 Drosophila melanogaster cullin 3, transcript variant D (Cul3), mRNA.

Price & Availability↑

CloneID	OFa15909
Clone ID Related Accession (Same CDS sequence)	NM_165110.2 , NM_165111.3 , NM_078849.3
Accession Version	NM_165111.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cullin 3, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165111.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTGC GGGGAAATCC TCCCAAGAAG GAGGGCAAAA TGCGCATTCG GGCCTTTCCG 
GCCTCCATGG ACGAGAAGTA CGTGGAGACC ATTTGGGCCA GCTTAAAGAA TGCTATCCAG 
GAGATACAGA AAAAGAACAA CTCCGGACTA TCGTTTGAAC AACTCTACCG GAATGCGTAC 
AACATGGTGC TGCATAAGCA CGGCAATAGA CTGTATTACG GTCTGAGGGA GGTCGTCTCA 
GAACACCTGG AGCACAAGGT GCGGGCGGAT GTGCTGGAGG CTCTGCACAG CAATTTCCTA 
CCCAAGTTAA ACCAGGCCTG GACAGACCAC CAGACATCTA TGGTGATGAT CCGCGACATA 
CTCATGTACA TGGATAGAGT TTATGTGCAG CAGCGCGAGG TAGACAATGT GTACAATTTG 
GGATTAATTT TGTTCAGGGA TCAGGTGGTT CGCTATTCTG AGATTCAAAA GGCCCTGCGA 
GAGAAGTTGC TGGGCATGGT GATGGAGGAA CGTCATGGCG AAGCCATTAA CCATCTGGCT 
ATCAAGAATG CTTGCAGTAT GCTGATCACC CTGGGGATTA ACTCGCGCAC TGTCTACGAA 
GAGGACTTTG AAAAGCCCTT CCTTGCGCAA TCGGCGGCGT TTTACAAATT CGAGTCGCAG 
AACTTTCTTG CCGAGAACAA CGCTGGCGTT TACATCAAGA AAGTGGAAGC GCGCATCACG 
GAGGAATCTT CCCGGGCAGC GCTGTATCTC GATAAGGATA CGGAGCCCCG CATTGTGCGC 
GTGGTCGAAG AAGAGCTGAT CAAAAAGCAC ATGCGACCCA TTGTTGAAAT GGAGAACTCG 
GGCGTGGTGT ACATGATCAA GAACTCAAAG ACCGAGGATC TGGCCTGCAC ATATAAGCTT 
TTCTCGCGCC TGAAGGAGGA GGGTCTCAAG GTGATAGCGG ACACAATGTC GGCATATCTG 
CGAGAGCAAG GACGCATGCT GGTTAAGGAA GAAGAAAACG GCAACACGAA TCCCATAACA 
TTCGTGCAGA ACTTGCTGGA TCTCAAGGAC CGCTTCGACC AGTTTCTGGT GCACTCGTTC 
GCCAACGATC GCATATTCAA AAACGTCATC TCATCCGATT TTGAACATTT CTTGAACCTA 
AATAACAAAT CCCCGGAGTA TCTATCGCTA TTCATCGATG ACAAACTGAA AAAGGGTGGC 
AAGGGAATGA GCGAACAGGA AATTGAGTCC ATCTTGGATA AGACAATGGT TCTCTTCCGT 
TTCCTATTGG AGAAAGATGT CTTTGAGCGC TATTACAAGA CGCATTTAGC CAAGAGATTG 
CTGCTGAACA AATCAGTCTC TGATGATTTC GAGAAGAATA TGATATCAAA ACTAAAGACT 
GAATGCGGCT GTCAATTCAC CTCGAAGCTA GAGGGCATGT TTAAAGATAT GTCAGTCTCC 
AATACGATCA TGGATGAGTT TAAGAACTTT GTAAATAATA ACAATTTATC CCTTGGCGGT 
GTGGAGCTAA CGGTACGCAT ACTAACCACT GGTTTTTGGC CCACACAGAC AGCAACTCCG 
AACTGCAATA TACCCGCCGC TCCGCGTGAA GCCTTTGACA TTTTCAAGAA CTTCTATCTA 
AATAAGCACT CAGGACGTCA ATTGACGTTA CAGCCACAAA TGGGAACCGC CTATATCAAC 
GCTGTGTTTT ATGGCCGCAA GGCAGTTGAA AGTGAAAAGG ATAAAGATGC ACCCAGCTCC 
AGTTCAAGCG GCTGTGGGGT GCCCACCACC ACACGCAAGC ACATATTGCA AGTGTCCACG 
TACCAGATGT GTGTGCTACT GCTGTTTAAT AACCGCGACG TGCTCACCTA CGATGACATA 
CACCAGGAGA CCGATATACC GGAACGGGAG CTCGTCCGAG CACTACAATC GTTGTCCATG 
GGCAAACCCG CTCAGCGTTT GTTAGTACGC AATTCTAAAA CGAAAACTAA GGACATTGAG 
CCCACGGATG AGTTTTACGT AAACGACGCC TTCAACTCCA AGTTCCACAG GGTGAAGATA 
CAAACGGTAG CCGCCAAGGG AGAATCAGAG CCAGAGCGTA AGGAAACGCG CGGAAAGGTC 
GACGAGGATC GTAAGCACGA GATCGAAGCG GCTATTGTGC GCATAATGAA GGCCCGCAAG 
CGTCTGGCTC ACAACTTGCT AGTATCAGAT GTGACGTCGC AGCTAAAGTC GCGTTTCTTG 
CCCTCGCCCG TGTTTATCAA AAAGCGCATT GAGGGCCTAA TCGAGCGCGA GTATCTGCAA 
CGATCGCCGG AAGATCGCAA AGTGTACAAC TACTTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723908.2
CDS	882..3203
Translation	MNLRGNPPKKEGKMRIRAFPASMDEKYVETIWASLKNAIQEIQKKNNSGLSFEQLYRNAYNMVLHKHGNRLYYGLREVVSEHLEHKVRADVLEALHSNFLPKLNQAWTDHQTSMVMIRDILMYMDRVYVQQREVDNVYNLGLILFRDQVVRYSEIQKALREKLLGMVMEERHGEAINHLAIKNACSMLITLGINSRTVYEEDFEKPFLAQSAAFYKFESQNFLAENNAGVYIKKVEARITEESSRAALYLDKDTEPRIVRVVEEELIKKHMRPIVEMENSGVVYMIKNSKTEDLACTYKLFSRLKEEGLKVIADTMSAYLREQGRMLVKEEENGNTNPITFVQNLLDLKDRFDQFLVHSFANDRIFKNVISSDFEHFLNLNNKSPEYLSLFIDDKLKKGGKGMSEQEIESILDKTMVLFRFLLEKDVFERYYKTHLAKRLLLNKSVSDDFEKNMISKLKTECGCQFTSKLEGMFKDMSVSNTIMDEFKNFVNNNNLSLGGVELTVRILTTGFWPTQTATPNCNIPAAPREAFDIFKNFYLNKHSGRQLTLQPQMGTAYINAVFYGRKAVESEKDKDAPSSSSSGCGVPTTTRKHILQVSTYQMCVLLLFNNRDVLTYDDIHQETDIPERELVRALQSLSMGKPAQRLLVRNSKTKTKDIEPTDEFYVNDAFNSKFHRVKIQTVAAKGESEPERKETRGKVDEDRKHEIEAAIVRIMKARKRLAHNLLVSDVTSQLKSRFLPSPVFIKKRIEGLIEREYLQRSPEDRKVYNYLA

Target ORF information:

RefSeq Version	NM_165111.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cullin 3, transcript variant D (Cul3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165111.3

ORF Insert Sequence:

ATGAATCTGC GGGGAAATCC TCCCAAGAAG GAGGGCAAAA TGCGCATTCG GGCCTTTCCG 
GCCTCCATGG ACGAGAAGTA CGTGGAGACC ATTTGGGCCA GCTTAAAGAA TGCTATCCAG 
GAGATACAGA AAAAGAACAA CTCCGGACTA TCGTTTGAAC AACTCTACCG GAATGCGTAC 
AACATGGTGC TGCATAAGCA CGGCAATAGA CTGTATTACG GTCTGAGGGA GGTCGTCTCA 
GAACACCTGG AGCACAAGGT GCGGGCGGAT GTGCTGGAGG CTCTGCACAG CAATTTCCTA 
CCCAAGTTAA ACCAGGCCTG GACAGACCAC CAGACATCTA TGGTGATGAT CCGCGACATA 
CTCATGTACA TGGATAGAGT TTATGTGCAG CAGCGCGAGG TAGACAATGT GTACAATTTG 
GGATTAATTT TGTTCAGGGA TCAGGTGGTT CGCTATTCTG AGATTCAAAA GGCCCTGCGA 
GAGAAGTTGC TGGGCATGGT GATGGAGGAA CGTCATGGCG AAGCCATTAA CCATCTGGCT 
ATCAAGAATG CTTGCAGTAT GCTGATCACC CTGGGGATTA ACTCGCGCAC TGTCTACGAA 
GAGGACTTTG AAAAGCCCTT CCTTGCGCAA TCGGCGGCGT TTTACAAATT CGAGTCGCAG 
AACTTTCTTG CCGAGAACAA CGCTGGCGTT TACATCAAGA AAGTGGAAGC GCGCATCACG 
GAGGAATCTT CCCGGGCAGC GCTGTATCTC GATAAGGATA CGGAGCCCCG CATTGTGCGC 
GTGGTCGAAG AAGAGCTGAT CAAAAAGCAC ATGCGACCCA TTGTTGAAAT GGAGAACTCG 
GGCGTGGTGT ACATGATCAA GAACTCAAAG ACCGAGGATC TGGCCTGCAC ATATAAGCTT 
TTCTCGCGCC TGAAGGAGGA GGGTCTCAAG GTGATAGCGG ACACAATGTC GGCATATCTG 
CGAGAGCAAG GACGCATGCT GGTTAAGGAA GAAGAAAACG GCAACACGAA TCCCATAACA 
TTCGTGCAGA ACTTGCTGGA TCTCAAGGAC CGCTTCGACC AGTTTCTGGT GCACTCGTTC 
GCCAACGATC GCATATTCAA AAACGTCATC TCATCCGATT TTGAACATTT CTTGAACCTA 
AATAACAAAT CCCCGGAGTA TCTATCGCTA TTCATCGATG ACAAACTGAA AAAGGGTGGC 
AAGGGAATGA GCGAACAGGA AATTGAGTCC ATCTTGGATA AGACAATGGT TCTCTTCCGT 
TTCCTATTGG AGAAAGATGT CTTTGAGCGC TATTACAAGA CGCATTTAGC CAAGAGATTG 
CTGCTGAACA AATCAGTCTC TGATGATTTC GAGAAGAATA TGATATCAAA ACTAAAGACT 
GAATGCGGCT GTCAATTCAC CTCGAAGCTA GAGGGCATGT TTAAAGATAT GTCAGTCTCC 
AATACGATCA TGGATGAGTT TAAGAACTTT GTAAATAATA ACAATTTATC CCTTGGCGGT 
GTGGAGCTAA CGGTACGCAT ACTAACCACT GGTTTTTGGC CCACACAGAC AGCAACTCCG 
AACTGCAATA TACCCGCCGC TCCGCGTGAA GCCTTTGACA TTTTCAAGAA CTTCTATCTA 
AATAAGCACT CAGGACGTCA ATTGACGTTA CAGCCACAAA TGGGAACCGC CTATATCAAC 
GCTGTGTTTT ATGGCCGCAA GGCAGTTGAA AGTGAAAAGG ATAAAGATGC ACCCAGCTCC 
AGTTCAAGCG GCTGTGGGGT GCCCACCACC ACACGCAAGC ACATATTGCA AGTGTCCACG 
TACCAGATGT GTGTGCTACT GCTGTTTAAT AACCGCGACG TGCTCACCTA CGATGACATA 
CACCAGGAGA CCGATATACC GGAACGGGAG CTCGTCCGAG CACTACAATC GTTGTCCATG 
GGCAAACCCG CTCAGCGTTT GTTAGTACGC AATTCTAAAA CGAAAACTAA GGACATTGAG 
CCCACGGATG AGTTTTACGT AAACGACGCC TTCAACTCCA AGTTCCACAG GGTGAAGATA 
CAAACGGTAG CCGCCAAGGG AGAATCAGAG CCAGAGCGTA AGGAAACGCG CGGAAAGGTC 
GACGAGGATC GTAAGCACGA GATCGAAGCG GCTATTGTGC GCATAATGAA GGCCCGCAAG 
CGTCTGGCTC ACAACTTGCT AGTATCAGAT GTGACGTCGC AGCTAAAGTC GCGTTTCTTG 
CCCTCGCCCG TGTTTATCAA AAAGCGCATT GAGGGCCTAA TCGAGCGCGA GTATCTGCAA 
CGATCGCCGG AAGATCGCAA AGTGTACAAC TACTTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul3 ( NM_078849.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523573.1 Drosophila melanogaster cullin 3, transcript variant C (Cul3), mRNA.

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CloneID	OFa15909
Clone ID Related Accession (Same CDS sequence)	NM_165110.2 , NM_165111.3 , NM_078849.3
Accession Version	NM_078849.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cullin 3, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078849.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTGC GGGGAAATCC TCCCAAGAAG GAGGGCAAAA TGCGCATTCG GGCCTTTCCG 
GCCTCCATGG ACGAGAAGTA CGTGGAGACC ATTTGGGCCA GCTTAAAGAA TGCTATCCAG 
GAGATACAGA AAAAGAACAA CTCCGGACTA TCGTTTGAAC AACTCTACCG GAATGCGTAC 
AACATGGTGC TGCATAAGCA CGGCAATAGA CTGTATTACG GTCTGAGGGA GGTCGTCTCA 
GAACACCTGG AGCACAAGGT GCGGGCGGAT GTGCTGGAGG CTCTGCACAG CAATTTCCTA 
CCCAAGTTAA ACCAGGCCTG GACAGACCAC CAGACATCTA TGGTGATGAT CCGCGACATA 
CTCATGTACA TGGATAGAGT TTATGTGCAG CAGCGCGAGG TAGACAATGT GTACAATTTG 
GGATTAATTT TGTTCAGGGA TCAGGTGGTT CGCTATTCTG AGATTCAAAA GGCCCTGCGA 
GAGAAGTTGC TGGGCATGGT GATGGAGGAA CGTCATGGCG AAGCCATTAA CCATCTGGCT 
ATCAAGAATG CTTGCAGTAT GCTGATCACC CTGGGGATTA ACTCGCGCAC TGTCTACGAA 
GAGGACTTTG AAAAGCCCTT CCTTGCGCAA TCGGCGGCGT TTTACAAATT CGAGTCGCAG 
AACTTTCTTG CCGAGAACAA CGCTGGCGTT TACATCAAGA AAGTGGAAGC GCGCATCACG 
GAGGAATCTT CCCGGGCAGC GCTGTATCTC GATAAGGATA CGGAGCCCCG CATTGTGCGC 
GTGGTCGAAG AAGAGCTGAT CAAAAAGCAC ATGCGACCCA TTGTTGAAAT GGAGAACTCG 
GGCGTGGTGT ACATGATCAA GAACTCAAAG ACCGAGGATC TGGCCTGCAC ATATAAGCTT 
TTCTCGCGCC TGAAGGAGGA GGGTCTCAAG GTGATAGCGG ACACAATGTC GGCATATCTG 
CGAGAGCAAG GACGCATGCT GGTTAAGGAA GAAGAAAACG GCAACACGAA TCCCATAACA 
TTCGTGCAGA ACTTGCTGGA TCTCAAGGAC CGCTTCGACC AGTTTCTGGT GCACTCGTTC 
GCCAACGATC GCATATTCAA AAACGTCATC TCATCCGATT TTGAACATTT CTTGAACCTA 
AATAACAAAT CCCCGGAGTA TCTATCGCTA TTCATCGATG ACAAACTGAA AAAGGGTGGC 
AAGGGAATGA GCGAACAGGA AATTGAGTCC ATCTTGGATA AGACAATGGT TCTCTTCCGT 
TTCCTATTGG AGAAAGATGT CTTTGAGCGC TATTACAAGA CGCATTTAGC CAAGAGATTG 
CTGCTGAACA AATCAGTCTC TGATGATTTC GAGAAGAATA TGATATCAAA ACTAAAGACT 
GAATGCGGCT GTCAATTCAC CTCGAAGCTA GAGGGCATGT TTAAAGATAT GTCAGTCTCC 
AATACGATCA TGGATGAGTT TAAGAACTTT GTAAATAATA ACAATTTATC CCTTGGCGGT 
GTGGAGCTAA CGGTACGCAT ACTAACCACT GGTTTTTGGC CCACACAGAC AGCAACTCCG 
AACTGCAATA TACCCGCCGC TCCGCGTGAA GCCTTTGACA TTTTCAAGAA CTTCTATCTA 
AATAAGCACT CAGGACGTCA ATTGACGTTA CAGCCACAAA TGGGAACCGC CTATATCAAC 
GCTGTGTTTT ATGGCCGCAA GGCAGTTGAA AGTGAAAAGG ATAAAGATGC ACCCAGCTCC 
AGTTCAAGCG GCTGTGGGGT GCCCACCACC ACACGCAAGC ACATATTGCA AGTGTCCACG 
TACCAGATGT GTGTGCTACT GCTGTTTAAT AACCGCGACG TGCTCACCTA CGATGACATA 
CACCAGGAGA CCGATATACC GGAACGGGAG CTCGTCCGAG CACTACAATC GTTGTCCATG 
GGCAAACCCG CTCAGCGTTT GTTAGTACGC AATTCTAAAA CGAAAACTAA GGACATTGAG 
CCCACGGATG AGTTTTACGT AAACGACGCC TTCAACTCCA AGTTCCACAG GGTGAAGATA 
CAAACGGTAG CCGCCAAGGG AGAATCAGAG CCAGAGCGTA AGGAAACGCG CGGAAAGGTC 
GACGAGGATC GTAAGCACGA GATCGAAGCG GCTATTGTGC GCATAATGAA GGCCCGCAAG 
CGTCTGGCTC ACAACTTGCT AGTATCAGAT GTGACGTCGC AGCTAAAGTC GCGTTTCTTG 
CCCTCGCCCG TGTTTATCAA AAAGCGCATT GAGGGCCTAA TCGAGCGCGA GTATCTGCAA 
CGATCGCCGG AAGATCGCAA AGTGTACAAC TACTTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523573.1
CDS	371..2692
Translation	MNLRGNPPKKEGKMRIRAFPASMDEKYVETIWASLKNAIQEIQKKNNSGLSFEQLYRNAYNMVLHKHGNRLYYGLREVVSEHLEHKVRADVLEALHSNFLPKLNQAWTDHQTSMVMIRDILMYMDRVYVQQREVDNVYNLGLILFRDQVVRYSEIQKALREKLLGMVMEERHGEAINHLAIKNACSMLITLGINSRTVYEEDFEKPFLAQSAAFYKFESQNFLAENNAGVYIKKVEARITEESSRAALYLDKDTEPRIVRVVEEELIKKHMRPIVEMENSGVVYMIKNSKTEDLACTYKLFSRLKEEGLKVIADTMSAYLREQGRMLVKEEENGNTNPITFVQNLLDLKDRFDQFLVHSFANDRIFKNVISSDFEHFLNLNNKSPEYLSLFIDDKLKKGGKGMSEQEIESILDKTMVLFRFLLEKDVFERYYKTHLAKRLLLNKSVSDDFEKNMISKLKTECGCQFTSKLEGMFKDMSVSNTIMDEFKNFVNNNNLSLGGVELTVRILTTGFWPTQTATPNCNIPAAPREAFDIFKNFYLNKHSGRQLTLQPQMGTAYINAVFYGRKAVESEKDKDAPSSSSSGCGVPTTTRKHILQVSTYQMCVLLLFNNRDVLTYDDIHQETDIPERELVRALQSLSMGKPAQRLLVRNSKTKTKDIEPTDEFYVNDAFNSKFHRVKIQTVAAKGESEPERKETRGKVDEDRKHEIEAAIVRIMKARKRLAHNLLVSDVTSQLKSRFLPSPVFIKKRIEGLIEREYLQRSPEDRKVYNYLA

Target ORF information:

RefSeq Version	NM_078849.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cullin 3, transcript variant C (Cul3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078849.3

ORF Insert Sequence:

ATGAATCTGC GGGGAAATCC TCCCAAGAAG GAGGGCAAAA TGCGCATTCG GGCCTTTCCG 
GCCTCCATGG ACGAGAAGTA CGTGGAGACC ATTTGGGCCA GCTTAAAGAA TGCTATCCAG 
GAGATACAGA AAAAGAACAA CTCCGGACTA TCGTTTGAAC AACTCTACCG GAATGCGTAC 
AACATGGTGC TGCATAAGCA CGGCAATAGA CTGTATTACG GTCTGAGGGA GGTCGTCTCA 
GAACACCTGG AGCACAAGGT GCGGGCGGAT GTGCTGGAGG CTCTGCACAG CAATTTCCTA 
CCCAAGTTAA ACCAGGCCTG GACAGACCAC CAGACATCTA TGGTGATGAT CCGCGACATA 
CTCATGTACA TGGATAGAGT TTATGTGCAG CAGCGCGAGG TAGACAATGT GTACAATTTG 
GGATTAATTT TGTTCAGGGA TCAGGTGGTT CGCTATTCTG AGATTCAAAA GGCCCTGCGA 
GAGAAGTTGC TGGGCATGGT GATGGAGGAA CGTCATGGCG AAGCCATTAA CCATCTGGCT 
ATCAAGAATG CTTGCAGTAT GCTGATCACC CTGGGGATTA ACTCGCGCAC TGTCTACGAA 
GAGGACTTTG AAAAGCCCTT CCTTGCGCAA TCGGCGGCGT TTTACAAATT CGAGTCGCAG 
AACTTTCTTG CCGAGAACAA CGCTGGCGTT TACATCAAGA AAGTGGAAGC GCGCATCACG 
GAGGAATCTT CCCGGGCAGC GCTGTATCTC GATAAGGATA CGGAGCCCCG CATTGTGCGC 
GTGGTCGAAG AAGAGCTGAT CAAAAAGCAC ATGCGACCCA TTGTTGAAAT GGAGAACTCG 
GGCGTGGTGT ACATGATCAA GAACTCAAAG ACCGAGGATC TGGCCTGCAC ATATAAGCTT 
TTCTCGCGCC TGAAGGAGGA GGGTCTCAAG GTGATAGCGG ACACAATGTC GGCATATCTG 
CGAGAGCAAG GACGCATGCT GGTTAAGGAA GAAGAAAACG GCAACACGAA TCCCATAACA 
TTCGTGCAGA ACTTGCTGGA TCTCAAGGAC CGCTTCGACC AGTTTCTGGT GCACTCGTTC 
GCCAACGATC GCATATTCAA AAACGTCATC TCATCCGATT TTGAACATTT CTTGAACCTA 
AATAACAAAT CCCCGGAGTA TCTATCGCTA TTCATCGATG ACAAACTGAA AAAGGGTGGC 
AAGGGAATGA GCGAACAGGA AATTGAGTCC ATCTTGGATA AGACAATGGT TCTCTTCCGT 
TTCCTATTGG AGAAAGATGT CTTTGAGCGC TATTACAAGA CGCATTTAGC CAAGAGATTG 
CTGCTGAACA AATCAGTCTC TGATGATTTC GAGAAGAATA TGATATCAAA ACTAAAGACT 
GAATGCGGCT GTCAATTCAC CTCGAAGCTA GAGGGCATGT TTAAAGATAT GTCAGTCTCC 
AATACGATCA TGGATGAGTT TAAGAACTTT GTAAATAATA ACAATTTATC CCTTGGCGGT 
GTGGAGCTAA CGGTACGCAT ACTAACCACT GGTTTTTGGC CCACACAGAC AGCAACTCCG 
AACTGCAATA TACCCGCCGC TCCGCGTGAA GCCTTTGACA TTTTCAAGAA CTTCTATCTA 
AATAAGCACT CAGGACGTCA ATTGACGTTA CAGCCACAAA TGGGAACCGC CTATATCAAC 
GCTGTGTTTT ATGGCCGCAA GGCAGTTGAA AGTGAAAAGG ATAAAGATGC ACCCAGCTCC 
AGTTCAAGCG GCTGTGGGGT GCCCACCACC ACACGCAAGC ACATATTGCA AGTGTCCACG 
TACCAGATGT GTGTGCTACT GCTGTTTAAT AACCGCGACG TGCTCACCTA CGATGACATA 
CACCAGGAGA CCGATATACC GGAACGGGAG CTCGTCCGAG CACTACAATC GTTGTCCATG 
GGCAAACCCG CTCAGCGTTT GTTAGTACGC AATTCTAAAA CGAAAACTAA GGACATTGAG 
CCCACGGATG AGTTTTACGT AAACGACGCC TTCAACTCCA AGTTCCACAG GGTGAAGATA 
CAAACGGTAG CCGCCAAGGG AGAATCAGAG CCAGAGCGTA AGGAAACGCG CGGAAAGGTC 
GACGAGGATC GTAAGCACGA GATCGAAGCG GCTATTGTGC GCATAATGAA GGCCCGCAAG 
CGTCTGGCTC ACAACTTGCT AGTATCAGAT GTGACGTCGC AGCTAAAGTC GCGTTTCTTG 
CCCTCGCCCG TGTTTATCAA AAAGCGCATT GAGGGCCTAA TCGAGCGCGA GTATCTGCAA 
CGATCGCCGG AAGATCGCAA AGTGTACAAC TACTTGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul3 ( NM_165112.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723909.2 Drosophila melanogaster cullin 3, transcript variant F (Cul3), mRNA.

Price & Availability↑

CloneID	OFa15910
Clone ID Related Accession (Same CDS sequence)	NM_165112.2
Accession Version	NM_165112.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cullin 3, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_165112.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAGGCC GCGATCCCCG CCAACAGCAG CCGGAGCCAC TGAACAACCT CAACGCCAAC 
GGCCGCTACC ACAATCGCAT GGTGGCCGGC TCCAACAACT TCAATGGCGC AACTGTCGGT 
AATGTGGACT CTGCCGTGCC TTGCCGGATT GCAAGGCAAA ACGTTCTTGG ACAGCAGGCG 
CGAAGCATGA ACATCAATGT GCCCAACGCC TCCTCAATGC AGCAACACCA GCAGTCCCAA 
AATCGATTGT CCGCTCAGGG ATTAGCTTCT GCCTCGCGGC GGAGTTATCA CCTTGCCGCC 
AGCGGTGGCA GAGTTCGGGC TGATAACAGA ATTAATACTA ACCACCCGCT CACGCCGACG 
CCACTTTCCA TGCCAGTTCC AGCTCCTGCT GCTCCAGCTG TCGTTAAAAC TGAAACTAGT 
GCAACCACCT CTGGGCCATC AACCTCAGCT TCAGCGAGCG CAGAAAGTAC AGAAAAACGC 
TTTAAGGAAA TAGCACGGAA GTACCCACTC TGGCTACCGG AGTACAAGCG AAGGGCATTC 
AATGCCTCCA TGGACGAGAA GTACGTGGAG ACCATTTGGG CCAGCTTAAA GAATGCTATC 
CAGGAGATAC AGAAAAAGAA CAACTCCGGA CTATCGTTTG AACAACTCTA CCGGAATGCG 
TACAACATGG TGCTGCATAA GCACGGCAAT AGACTGTATT ACGGTCTGAG GGAGGTCGTC 
TCAGAACACC TGGAGCACAA GGTGCGGGCG GATGTGCTGG AGGCTCTGCA CAGCAATTTC 
CTACCCAAGT TAAACCAGGC CTGGACAGAC CACCAGACAT CTATGGTGAT GATCCGCGAC 
ATACTCATGT ACATGGATAG AGTTTATGTG CAGCAGCGCG AGGTAGACAA TGTGTACAAT 
TTGGGATTAA TTTTGTTCAG GGATCAGGTG GTTCGCTATT CTGAGATTCA AAAGGCCCTG 
CGAGAGAAGT TGCTGGGCAT GGTGATGGAG GAACGTCATG GCGAAGCCAT TAACCATCTG 
GCTATCAAGA ATGCTTGCAG TATGCTGATC ACCCTGGGGA TTAACTCGCG CACTGTCTAC 
GAAGAGGACT TTGAAAAGCC CTTCCTTGCG CAATCGGCGG CGTTTTACAA ATTCGAGTCG 
CAGAACTTTC TTGCCGAGAA CAACGCTGGC GTTTACATCA AGAAAGTGGA AGCGCGCATC 
ACGGAGGAAT CTTCCCGGGC AGCGCTGTAT CTCGATAAGG ATACGGAGCC CCGCATTGTG 
CGCGTGGTCG AAGAAGAGCT GATCAAAAAG CACATGCGAC CCATTGTTGA AATGGAGAAC 
TCGGGCGTGG TGTACATGAT CAAGAACTCA AAGACCGAGG ATCTGGCCTG CACATATAAG 
CTTTTCTCGC GCCTGAAGGA GGAGGGTCTC AAGGTGATAG CGGACACAAT GTCGGCATAT 
CTGCGAGAGC AAGGACGCAT GCTGGTTAAG GAAGAAGAAA ACGGCAACAC GAATCCCATA 
ACATTCGTGC AGAACTTGCT GGATCTCAAG GACCGCTTCG ACCAGTTTCT GGTGCACTCG 
TTCGCCAACG ATCGCATATT CAAAAACGTC ATCTCATCCG ATTTTGAACA TTTCTTGAAC 
CTAAATAACA AATCCCCGGA GTATCTATCG CTATTCATCG ATGACAAACT GAAAAAGGGT 
GGCAAGGGAA TGAGCGAACA GGAAATTGAG TCCATCTTGG ATAAGACAAT GGTTCTCTTC 
CGTTTCCTAT TGGAGAAAGA TGTCTTTGAG CGCTATTACA AGACGCATTT AGCCAAGAGA 
TTGCTGCTGA ACAAATCAGT CTCTGATGAT TTCGAGAAGA ATATGATATC AAAACTAAAG 
ACTGAATGCG GCTGTCAATT CACCTCGAAG CTAGAGGGCA TGTTTAAAGA TATGTCAGTC 
TCCAATACGA TCATGGATGA GTTTAAGAAC TTTGTAAATA ATAACAATTT ATCCCTTGGC 
GGTGTGGAGC TAACGGTACG CATACTAACC ACTGGTTTTT GGCCCACACA GACAGCAACT 
CCGAACTGCA ATATACCCGC CGCTCCGCGT GAAGCCTTTG ACATTTTCAA GAACTTCTAT 
CTAAATAAGC ACTCAGGACG TCAATTGACG TTACAGCCAC AAATGGGAAC CGCCTATATC 
AACGCTGTGT TTTATGGCCG CAAGGCAGTT GAAAGTGAAA AGGATAAAGA TGCACCCAGC 
TCCAGTTCAA GCGGCTGTGG GGTGCCCACC ACCACACGCA AGCACATATT GCAAGTGTCC 
ACGTACCAGA TGTGTGTGCT ACTGCTGTTT AATAACCGCG ACGTGCTCAC CTACGATGAC 
ATACACCAGG AGACCGATAT ACCGGAACGG GAGCTCGTCC GAGCACTACA ATCGTTGTCC 
ATGGGCAAAC CCGCTCAGCG TTTGTTAGTA CGCAATTCTA AAACGAAAAC TAAGGACATT 
GAGCCCACGG ATGAGTTTTA CGTAAACGAC GCCTTCAACT CCAAGTTCCA CAGGGTGAAG 
ATACAAACGG TAGCCGCCAA GGGAGAATCA GAGCCAGAGC GTAAGGAAAC GCGCGGAAAG 
GTCGACGAGG ATCGTAAGCA CGAGATCGAA GCGGCTATTG TGCGCATAAT GAAGGCCCGC 
AAGCGTCTGG CTCACAACTT GCTAGTATCA GATGTGACGT CGCAGCTAAA GTCGCGTTTC 
TTGCCCTCGC CCGTGTTTAT CAAAAAGCGC ATTGAGGGCC TAATCGAGCG CGAGTATCTG 
CAACGATCGC CGGAAGATCG CAAAGTGTAC AACTACTTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723909.2
CDS	409..3213
Translation	MQGRDPRQQQPEPLNNLNANGRYHNRMVAGSNNFNGATVGNVDSAVPCRIARQNVLGQQARSMNINVPNASSMQQHQQSQNRLSAQGLASASRRSYHLAASGGRVRADNRINTNHPLTPTPLSMPVPAPAAPAVVKTETSATTSGPSTSASASAESTEKRFKEIARKYPLWLPEYKRRAFNASMDEKYVETIWASLKNAIQEIQKKNNSGLSFEQLYRNAYNMVLHKHGNRLYYGLREVVSEHLEHKVRADVLEALHSNFLPKLNQAWTDHQTSMVMIRDILMYMDRVYVQQREVDNVYNLGLILFRDQVVRYSEIQKALREKLLGMVMEERHGEAINHLAIKNACSMLITLGINSRTVYEEDFEKPFLAQSAAFYKFESQNFLAENNAGVYIKKVEARITEESSRAALYLDKDTEPRIVRVVEEELIKKHMRPIVEMENSGVVYMIKNSKTEDLACTYKLFSRLKEEGLKVIADTMSAYLREQGRMLVKEEENGNTNPITFVQNLLDLKDRFDQFLVHSFANDRIFKNVISSDFEHFLNLNNKSPEYLSLFIDDKLKKGGKGMSEQEIESILDKTMVLFRFLLEKDVFERYYKTHLAKRLLLNKSVSDDFEKNMISKLKTECGCQFTSKLEGMFKDMSVSNTIMDEFKNFVNNNNLSLGGVELTVRILTTGFWPTQTATPNCNIPAAPREAFDIFKNFYLNKHSGRQLTLQPQMGTAYINAVFYGRKAVESEKDKDAPSSSSSGCGVPTTTRKHILQVSTYQMCVLLLFNNRDVLTYDDIHQETDIPERELVRALQSLSMGKPAQRLLVRNSKTKTKDIEPTDEFYVNDAFNSKFHRVKIQTVAAKGESEPERKETRGKVDEDRKHEIEAAIVRIMKARKRLAHNLLVSDVTSQLKSRFLPSPVFIKKRIEGLIEREYLQRSPEDRKVYNYLA

Target ORF information:

RefSeq Version	NM_165112.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cullin 3, transcript variant F (Cul3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165112.2

ORF Insert Sequence:

ATGCAAGGCC GCGATCCCCG CCAACAGCAG CCGGAGCCAC TGAACAACCT CAACGCCAAC 
GGCCGCTACC ACAATCGCAT GGTGGCCGGC TCCAACAACT TCAATGGCGC AACTGTCGGT 
AATGTGGACT CTGCCGTGCC TTGCCGGATT GCAAGGCAAA ACGTTCTTGG ACAGCAGGCG 
CGAAGCATGA ACATCAATGT GCCCAACGCC TCCTCAATGC AGCAACACCA GCAGTCCCAA 
AATCGATTGT CCGCTCAGGG ATTAGCTTCT GCCTCGCGGC GGAGTTATCA CCTTGCCGCC 
AGCGGTGGCA GAGTTCGGGC TGATAACAGA ATTAATACTA ACCACCCGCT CACGCCGACG 
CCACTTTCCA TGCCAGTTCC AGCTCCTGCT GCTCCAGCTG TCGTTAAAAC TGAAACTAGT 
GCAACCACCT CTGGGCCATC AACCTCAGCT TCAGCGAGCG CAGAAAGTAC AGAAAAACGC 
TTTAAGGAAA TAGCACGGAA GTACCCACTC TGGCTACCGG AGTACAAGCG AAGGGCATTC 
AATGCCTCCA TGGACGAGAA GTACGTGGAG ACCATTTGGG CCAGCTTAAA GAATGCTATC 
CAGGAGATAC AGAAAAAGAA CAACTCCGGA CTATCGTTTG AACAACTCTA CCGGAATGCG 
TACAACATGG TGCTGCATAA GCACGGCAAT AGACTGTATT ACGGTCTGAG GGAGGTCGTC 
TCAGAACACC TGGAGCACAA GGTGCGGGCG GATGTGCTGG AGGCTCTGCA CAGCAATTTC 
CTACCCAAGT TAAACCAGGC CTGGACAGAC CACCAGACAT CTATGGTGAT GATCCGCGAC 
ATACTCATGT ACATGGATAG AGTTTATGTG CAGCAGCGCG AGGTAGACAA TGTGTACAAT 
TTGGGATTAA TTTTGTTCAG GGATCAGGTG GTTCGCTATT CTGAGATTCA AAAGGCCCTG 
CGAGAGAAGT TGCTGGGCAT GGTGATGGAG GAACGTCATG GCGAAGCCAT TAACCATCTG 
GCTATCAAGA ATGCTTGCAG TATGCTGATC ACCCTGGGGA TTAACTCGCG CACTGTCTAC 
GAAGAGGACT TTGAAAAGCC CTTCCTTGCG CAATCGGCGG CGTTTTACAA ATTCGAGTCG 
CAGAACTTTC TTGCCGAGAA CAACGCTGGC GTTTACATCA AGAAAGTGGA AGCGCGCATC 
ACGGAGGAAT CTTCCCGGGC AGCGCTGTAT CTCGATAAGG ATACGGAGCC CCGCATTGTG 
CGCGTGGTCG AAGAAGAGCT GATCAAAAAG CACATGCGAC CCATTGTTGA AATGGAGAAC 
TCGGGCGTGG TGTACATGAT CAAGAACTCA AAGACCGAGG ATCTGGCCTG CACATATAAG 
CTTTTCTCGC GCCTGAAGGA GGAGGGTCTC AAGGTGATAG CGGACACAAT GTCGGCATAT 
CTGCGAGAGC AAGGACGCAT GCTGGTTAAG GAAGAAGAAA ACGGCAACAC GAATCCCATA 
ACATTCGTGC AGAACTTGCT GGATCTCAAG GACCGCTTCG ACCAGTTTCT GGTGCACTCG 
TTCGCCAACG ATCGCATATT CAAAAACGTC ATCTCATCCG ATTTTGAACA TTTCTTGAAC 
CTAAATAACA AATCCCCGGA GTATCTATCG CTATTCATCG ATGACAAACT GAAAAAGGGT 
GGCAAGGGAA TGAGCGAACA GGAAATTGAG TCCATCTTGG ATAAGACAAT GGTTCTCTTC 
CGTTTCCTAT TGGAGAAAGA TGTCTTTGAG CGCTATTACA AGACGCATTT AGCCAAGAGA 
TTGCTGCTGA ACAAATCAGT CTCTGATGAT TTCGAGAAGA ATATGATATC AAAACTAAAG 
ACTGAATGCG GCTGTCAATT CACCTCGAAG CTAGAGGGCA TGTTTAAAGA TATGTCAGTC 
TCCAATACGA TCATGGATGA GTTTAAGAAC TTTGTAAATA ATAACAATTT ATCCCTTGGC 
GGTGTGGAGC TAACGGTACG CATACTAACC ACTGGTTTTT GGCCCACACA GACAGCAACT 
CCGAACTGCA ATATACCCGC CGCTCCGCGT GAAGCCTTTG ACATTTTCAA GAACTTCTAT 
CTAAATAAGC ACTCAGGACG TCAATTGACG TTACAGCCAC AAATGGGAAC CGCCTATATC 
AACGCTGTGT TTTATGGCCG CAAGGCAGTT GAAAGTGAAA AGGATAAAGA TGCACCCAGC 
TCCAGTTCAA GCGGCTGTGG GGTGCCCACC ACCACACGCA AGCACATATT GCAAGTGTCC 
ACGTACCAGA TGTGTGTGCT ACTGCTGTTT AATAACCGCG ACGTGCTCAC CTACGATGAC 
ATACACCAGG AGACCGATAT ACCGGAACGG GAGCTCGTCC GAGCACTACA ATCGTTGTCC 
ATGGGCAAAC CCGCTCAGCG TTTGTTAGTA CGCAATTCTA AAACGAAAAC TAAGGACATT 
GAGCCCACGG ATGAGTTTTA CGTAAACGAC GCCTTCAACT CCAAGTTCCA CAGGGTGAAG 
ATACAAACGG TAGCCGCCAA GGGAGAATCA GAGCCAGAGC GTAAGGAAAC GCGCGGAAAG 
GTCGACGAGG ATCGTAAGCA CGAGATCGAA GCGGCTATTG TGCGCATAAT GAAGGCCCGC 
AAGCGTCTGG CTCACAACTT GCTAGTATCA GATGTGACGT CGCAGCTAAA GTCGCGTTTC 
TTGCCCTCGC CCGTGTTTAT CAAAAAGCGC ATTGAGGGCC TAATCGAGCG CGAGTATCTG 
CAACGATCGC CGGAAGATCG CAAAGTGTAC AACTACTTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cul3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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