rk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	rk
Entrez Gene ID	34819
Full Name	rickets
Synonyms	BG:DS00180.13,CG8930,CT25644,DLGR-2,DLGR2,Dmel\CG8930,LGR2,lgr2,rk/CG8930
General protein information	Preferred Names rickets Names CG8930 gene product from transcript CG8930-RA CG8930-PA glycoprotein hormone receptor II glycoprotein-hormone-receptor-II leucine-rich repeat-containing G protein-coupled receptor 2 rk-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34E5-34E6\|2-48.8 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057354.3	NP_476702.1	rickets

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LGR5	XP_003313909.1
	LGR5	XP_001117502.1
Drosophila melanogaster (fruit fly)	rk	NP_476702.1
Mus musculus (house mouse)	Lgr5	NP_034325.2
Homo sapiens (human)	LGR5	NP_003658.1
Gallus gallus (chicken)	LGR5	XP_425441.2
Rattus norvegicus (Norway rat)	Lgr5	NP_001100254.1
Bos taurus (cattle)	LGR5	NP_001179449.1
Anopheles gambiae (African malaria mosquito)	GPRRK	XP_317111.1
Xenopus tropicalis (tropical clawed frog)	lgr5	XP_002940219.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In Drosophila, rk function is required for the normal maturation of the fly's cuticular hydrocarbon (CH) profile, with flies mutant for rk function showing dramatically elevated levels of CHs. This effect is mostly abrogated by mutations in the Delta9 desaturase encoded by the desaturase1 gene, suggesting that desaturase1 acts downstream of rk.
Title: Regulation of cuticular hydrocarbon profile maturation by Drosophila tanning hormone, bursicon, and its interaction with desaturase activity.

2 alleles of the gene rickets (rk) encoding a G-protein-coupled receptor were isolated. The rk alleles result in border cell migration defects in a significant fraction of egg chambers. The rk receptor appears to regulate detachment and polarity of the border cell cluster coordinating processes within the cells of the cluster themselves.
Title: Signaling through the G-protein-coupled receptor Rickets is important for polarity, detachment, and migration of the border cells in Drosophila.

Membrane-anchored bursicon constructs were generated that can activate or inhibit membrane rk signaling.
Title: Membrane tethered bursicon constructs as heterodimeric modulators of the Drosophila G protein-coupled receptor rickets.

Bursicon is a heterodimeric cystine knot protein that activates G protein-coupled receptor LGR2.
Title: Bursicon, the insect cuticle-hardening hormone, is a heterodimeric cystine knot protein that activates G protein-coupled receptor LGR2.

rickets appears to encode a component of the bursicon response pathway
Title: Mutations in the Drosophila glycoprotein hormone receptor, rickets, eliminate neuropeptide-induced tanning and selectively block a stereotyped behavioral program.

The following rk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16759	NM_057354.3 Latest version!	Drosophila melanogaster rickets (rk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rk ( NM_057354.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476702.1 Drosophila melanogaster rickets (rk), mRNA.

Price & Availability↑

CloneID	OFa16759
Clone ID Related Accession (Same CDS sequence)	NM_057354.3
Accession Version	NM_057354.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rickets
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057354.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAC GTTGCCGGTG GAGCTGGCGC CTGGCGCTCT GCCCTCTGCT GTTGCAATTG 
CTTCTGCAAC TGCTCCTGTT GCCGCCATCG GCAATGGGCC ATGATGAAAC CAAAGAAAAT 
CCCGCTCCGG ATATGCAAAA TAGCCAGGAG CAAGAGCCAT ATGTCCACCT GCAACATCTG 
CAGCAGCAGC AGCAGCAAAA TCCACAAACC GTCCAACAAC TTAGCCAAAT TACCGTGAAT 
AGAACCTCGA AATCAGCCAG TGTCACTCCT ACGGGCATCA GGGAGAACGT GATGCTGCCA 
TCGGCCGATC CCGAGAAGGA GGCCCAGATC CTGTACGAGA AGTCCTTGCA GGAATACCAT 
GGCAGTCAGC TCTCCACCGC CTCAACCGCC ACTGATGTCA TCGCAGGCAA GCGAACGCTG 
CACTCCATTT GTGAGCGGTG GTTGCAGAAG CACTGTCACT GCACCGGCAG CCTGGAGGTC 
CTGCGGCTCA GTTGTCGGGG CATAGGTATC CTGGCCGTGC CCGTCAATCT TCCCAACGAG 
GTGGTCGTCC TAGATTTGGG TAACAACAAC TTAACCAAAT TGGAGGCGAA CTCATTTTTT 
ATGGCACCCA ATCTGGAGGA GCTAACTTTG TCCGACAATA GCATCATTAA TATGGATCCC 
AATGCATTCT ATGGCCTGGC CAAATTGAAA CGATTGAGCC TGCAGAACTG TGGCCTCAAG 
TCCTTGCCAC CGCAGTCGTT CCAAGGACTT GCTCAGCTGA CCAGCCTACA GCTGAATGGC 
AACGCCCTGG TCAGTCTGGA TGGCGATTGT CTGGGCCACC TGCAGAAACT GCGCACCTTG 
CGATTGGAGG GCAATCTCTT CTATCGCATT CCCACGAATG CCTTGGCCGG ACTCAGAACC 
CTAGAAGCAC TCAATTTGGG CAGCAATTTG TTGACAATAA TAAACGACGA GGACTTTCCG 
CGAATGCCAA ACTTGATCGT GCTTTTGCTG AAGCGAAATC AAATCATGAA AATCTCTGCG 
GGAGCTCTTA AAAATTTAAC AGCCTTAAAA GTTTTAGAGC TGGACGATAA TTTAATAAGC 
AGCCTGCCCG AGGGACTCAG CAAATTGTCG CAACTGCAGG AGCTTTCCAT CACCAGCAAT 
CGACTGCGCT GGATAAACGA CACGGAACTG CCCAGGAGCA TGCAAATGCT GGACATGAGG 
GCCAATCCAC TGTCCACAAT TTCGCCAGGG GCGTTTCGGG GCATGTCCAA GTTGCGGAAG 
CTGATTTTAT CGGATGTGCG AACTTTGCGC TCGTTTCCCG AACTTGAGGC CTGCCATGCG 
CTGGAAATAC TGAAACTGGA TCGTGCCGGC ATTCAGGAGG TGCCAGCGAA TCTATGCCGC 
CAAACGCCAA GACTGAAGAG TTTAGAACTA AAAACCAATT CCCTAAAACG CATCCCAAAT 
TTAAGTAGCT GCAGAGACTT AAGGCTGTTA GATCTATCGA GCAATCAAAT TGAAAAGATT 
CAGGGAAAGC CCTTCAATGG ATTGAAGCAA TTGAACGATC TGTTGCTCTC CTACAATCGA 
ATCAAGGCAC TGCCACAAGA TGCCTTTCAG GGCATTCCGA AGCTCCAACT TCTTGACCTG 
GAGGGCAACG AGATCTCGTA CATACACAAG GAAGCCTTTT CCGGTTTCAC AGCCCTCGAG 
GATCTCAATT TGGGCAACAA TATTTTTCCA GAGTTGCCCG AATCGGGACT GCGCGCTTTG 
CTACACCTAA AGACCTTCAA CAATCCAAAA CTTAGGGAGT TCCCGCCACC GGACACCTTT 
CCCAGAATCC AAACTCTTAT ACTCTCTTAT GCCTACCACT GCTGCGCGTT TCTACCACTG 
GTTGCCATGT CGTCCCAGAA GAAAACCTCC CAGGTCCAGG AGGCTGTACT CTTTCCCTCC 
GATGCCGAGT TCGATATGAC TTTGTGGAAC AATAGCATGA TGAATATTTG GCCGCAAATG 
CATAATCTCA GCAAGCAGTT GGGTGCTTCC ATGCATGATC CTTGGGAGAC GGCTATTAAC 
TTCAACGAAG AGCAGCTGCA AACACAAACG GGAGGCCAAA TCGCCACCAG CTACATGGAG 
GAGTACTTCG AGGAGCACGA TGTGAGTGGT CCTGCCACGG GATACGGCTT TGGTACTGGA 
CTATTCTCTG GTATGTCCAC AGAGGACTTT CAACCGGGTT CGGTGCAGTG CCTGCCAATG 
CCAGGACCTT TTCTACCCTG CGCCGATCTC TTCGATTGGT GGACTCTGCG CTGCGGAGTG 
TGGGTGGTTT TTCTGTTGTC TCTCCTGGGC AACGGAACGG TGGTGTTTGT GCTGCTCTGC 
TCGCGATCAA AGATGGATGT GCCCCGATTT CTAGTCTGTA ATCTGGCCGC GGCCGACTTC 
TTCATGGGCA TCTACCTGGG TATTCTGGCC ATTGTGGATG CGGCGACGTT GGGCGAATTT 
CGCATGTTTG CCATTCCTTG GCAGATGTCC GTGCTCTGCC AGTTGTCCGG TTTTCTGGCC 
GTACTCAGCT CAGAATTATC CGTTTACACG TTGGCTGTGA TCACTTTGGA GCGCAATTAC 
GCCATCACTC ATGCCATTCA CCTGAACAAG AGGCTCTCCT TGAAGCAGGC GGGATATATA 
ATGAGTGTAG GATGGGTTTT TGCCCTGATC ATGGCTTTGA TGCCTTTGGT TGGTGTTTCG 
GACTACAGGA AGTTTGCCGT GTGTTTGCCA TTCGAGACCA CCACTGGACC GGCCAGTTTG 
ACCTATGTAA TCTCACTGAT GTTCATCAAC GGATGCGCAT TTCTCACTCT GATGGGTTGC 
TACCTGAAGA TGTACTGGGC CATAAGGGGC AGCCAGGCAT GGAACACGAA TGATTCGCGA 
ATTGCCAAGC GAATGGCTCT GTTGGTCTTT ACTGACTTCC TCTGCTGGTC ACCTATCGCC 
TTCTTCTCGA TCACTGCCAT CTTTGGCCTG CAACTGATTT CGCTGGAGCA GGCCAAGATC 
TTTACGGTAT TTGTGCTGCC CTTGAATAGT TGCTGCAATC CCTTTCTCTA CGCCATAATG 
ACCAAGCAGT TCAAGAAGGA CTGTGTGACG TTGTGCAAAC ATTTCGAAGA ATCCCGCGTC 
GTGGGAGGCG GTGGTCCGGG TGGACGTGGT GCAGTGGCAA GAACCAAGAG GGGTGATCTC 
CCGCCACCAC TCTTACCAGC TGCAGCGGTG GCACATCCGC CAGGCTGTCG ATGTCTACGG 
ATGCTGCCCA GCGAAATGCC CAACTGGCAT AAAATGGAGC AGACACCGAG CATGTGGCAG 
AGATTGAGAA CATTCTGCTG CGGGGAGAAC CGAAGGCGTC GCAAACAGCG ACGGCAACCG 
CAACAGCGAC GCCAGAGAGC CTATACTGCC GCAGCCGCCA ATCCGTATCA GTATCAGTTT 
GCTGAACTGC GTCAGCAACG CCAGAATCGA GCCAGTTCCA TTTCCAGCGA GAATTTCTGC 
AGCTCAAGAT CATCCAGCTG GCGACATGGT CCACCATCCT CAGCACCCGT GCCACCGGGT 
AACTGCAGCA TGCCGCTGAA GATGCTGGAG CCACATGCTC ATCCGCACGG TCATGGGCGC 
AGACGACACT CCGCCTGGCT GATCACGCGG AAAACGTCGC AGGACTCGAA CTTGTCCAGC 
TCGCGCAATG ACTCCTCCGC CTCGGCCACC ACCGCCAGCA CCTCCACGTT CCGGTTATCC 
CGGTCAAGTG CCGGCAGCAG CACACCCCTG CCCTCGATCA TAGCCCACAA CGGGAAGGCC 
CAGTTGGATG CAGTTAAACC ACGATTGGTC CGCCAGGAAG CAGTCCAGGA GGAAGAGGAC 
TCATCACCAC CTCGCCTGGG AGTACGCTTC CTACCCACCA TACCCTCGGC TGCCGACAGT 
TCTGTCGTCA TGGAAGATGG CGACTCGGCC AACACGGGAG TAGCCAGTTT TCTAGGCATG 
CCACTTCCTG GGGCGTCCAG TGGCTTCCTT ATAGCTCCAA CCACCGCTGC CACCACCTCC 
CCACCCCCGG TGGTCCTGCA GCCGGCAAAG CCTCCTCCCG ATCCCAACGA TGCACCACTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476702.1
CDS	625..4707
Translation	MAARCRWSWRLALCPLLLQLLLQLLLLPPSAMGHDETKENPAPDMQNSQEQEPYVHLQHLQQQQQQNPQTVQQLSQITVNRTSKSASVTPTGIRENVMLPSADPEKEAQILYEKSLQEYHGSQLSTASTATDVIAGKRTLHSICERWLQKHCHCTGSLEVLRLSCRGIGILAVPVNLPNEVVVLDLGNNNLTKLEANSFFMAPNLEELTLSDNSIINMDPNAFYGLAKLKRLSLQNCGLKSLPPQSFQGLAQLTSLQLNGNALVSLDGDCLGHLQKLRTLRLEGNLFYRIPTNALAGLRTLEALNLGSNLLTIINDEDFPRMPNLIVLLLKRNQIMKISAGALKNLTALKVLELDDNLISSLPEGLSKLSQLQELSITSNRLRWINDTELPRSMQMLDMRANPLSTISPGAFRGMSKLRKLILSDVRTLRSFPELEACHALEILKLDRAGIQEVPANLCRQTPRLKSLELKTNSLKRIPNLSSCRDLRLLDLSSNQIEKIQGKPFNGLKQLNDLLLSYNRIKALPQDAFQGIPKLQLLDLEGNEISYIHKEAFSGFTALEDLNLGNNIFPELPESGLRALLHLKTFNNPKLREFPPPDTFPRIQTLILSYAYHCCAFLPLVAMSSQKKTSQVQEAVLFPSDAEFDMTLWNNSMMNIWPQMHNLSKQLGASMHDPWETAINFNEEQLQTQTGGQIATSYMEEYFEEHDVSGPATGYGFGTGLFSGMSTEDFQPGSVQCLPMPGPFLPCADLFDWWTLRCGVWVVFLLSLLGNGTVVFVLLCSRSKMDVPRFLVCNLAAADFFMGIYLGILAIVDAATLGEFRMFAIPWQMSVLCQLSGFLAVLSSELSVYTLAVITLERNYAITHAIHLNKRLSLKQAGYIMSVGWVFALIMALMPLVGVSDYRKFAVCLPFETTTGPASLTYVISLMFINGCAFLTLMGCYLKMYWAIRGSQAWNTNDSRIAKRMALLVFTDFLCWSPIAFFSITAIFGLQLISLEQAKIFTVFVLPLNSCCNPFLYAIMTKQFKKDCVTLCKHFEESRVVGGGGPGGRGAVARTKRGDLPPPLLPAAAVAHPPGCRCLRMLPSEMPNWHKMEQTPSMWQRLRTFCCGENRRRRKQRRQPQQRRQRAYTAAAANPYQYQFAELRQQRQNRASSISSENFCSSRSSSWRHGPPSSAPVPPGNCSMPLKMLEPHAHPHGHGRRRHSAWLITRKTSQDSNLSSSRNDSSASATTASTSTFRLSRSSAGSSTPLPSIIAHNGKAQLDAVKPRLVRQEAVQEEEDSSPPRLGVRFLPTIPSAADSSVVMEDGDSANTGVASFLGMPLPGASSGFLIAPTTAATTSPPPVVLQPAKPPPDPNDAPL

Target ORF information:

RefSeq Version	NM_057354.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rickets (rk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057354.3

ORF Insert Sequence:

ATGGCAGCAC GTTGCCGGTG GAGCTGGCGC CTGGCGCTCT GCCCTCTGCT GTTGCAATTG 
CTTCTGCAAC TGCTCCTGTT GCCGCCATCG GCAATGGGCC ATGATGAAAC CAAAGAAAAT 
CCCGCTCCGG ATATGCAAAA TAGCCAGGAG CAAGAGCCAT ATGTCCACCT GCAACATCTG 
CAGCAGCAGC AGCAGCAAAA TCCACAAACC GTCCAACAAC TTAGCCAAAT TACCGTGAAT 
AGAACCTCGA AATCAGCCAG TGTCACTCCT ACGGGCATCA GGGAGAACGT GATGCTGCCA 
TCGGCCGATC CCGAGAAGGA GGCCCAGATC CTGTACGAGA AGTCCTTGCA GGAATACCAT 
GGCAGTCAGC TCTCCACCGC CTCAACCGCC ACTGATGTCA TCGCAGGCAA GCGAACGCTG 
CACTCCATTT GTGAGCGGTG GTTGCAGAAG CACTGTCACT GCACCGGCAG CCTGGAGGTC 
CTGCGGCTCA GTTGTCGGGG CATAGGTATC CTGGCCGTGC CCGTCAATCT TCCCAACGAG 
GTGGTCGTCC TAGATTTGGG TAACAACAAC TTAACCAAAT TGGAGGCGAA CTCATTTTTT 
ATGGCACCCA ATCTGGAGGA GCTAACTTTG TCCGACAATA GCATCATTAA TATGGATCCC 
AATGCATTCT ATGGCCTGGC CAAATTGAAA CGATTGAGCC TGCAGAACTG TGGCCTCAAG 
TCCTTGCCAC CGCAGTCGTT CCAAGGACTT GCTCAGCTGA CCAGCCTACA GCTGAATGGC 
AACGCCCTGG TCAGTCTGGA TGGCGATTGT CTGGGCCACC TGCAGAAACT GCGCACCTTG 
CGATTGGAGG GCAATCTCTT CTATCGCATT CCCACGAATG CCTTGGCCGG ACTCAGAACC 
CTAGAAGCAC TCAATTTGGG CAGCAATTTG TTGACAATAA TAAACGACGA GGACTTTCCG 
CGAATGCCAA ACTTGATCGT GCTTTTGCTG AAGCGAAATC AAATCATGAA AATCTCTGCG 
GGAGCTCTTA AAAATTTAAC AGCCTTAAAA GTTTTAGAGC TGGACGATAA TTTAATAAGC 
AGCCTGCCCG AGGGACTCAG CAAATTGTCG CAACTGCAGG AGCTTTCCAT CACCAGCAAT 
CGACTGCGCT GGATAAACGA CACGGAACTG CCCAGGAGCA TGCAAATGCT GGACATGAGG 
GCCAATCCAC TGTCCACAAT TTCGCCAGGG GCGTTTCGGG GCATGTCCAA GTTGCGGAAG 
CTGATTTTAT CGGATGTGCG AACTTTGCGC TCGTTTCCCG AACTTGAGGC CTGCCATGCG 
CTGGAAATAC TGAAACTGGA TCGTGCCGGC ATTCAGGAGG TGCCAGCGAA TCTATGCCGC 
CAAACGCCAA GACTGAAGAG TTTAGAACTA AAAACCAATT CCCTAAAACG CATCCCAAAT 
TTAAGTAGCT GCAGAGACTT AAGGCTGTTA GATCTATCGA GCAATCAAAT TGAAAAGATT 
CAGGGAAAGC CCTTCAATGG ATTGAAGCAA TTGAACGATC TGTTGCTCTC CTACAATCGA 
ATCAAGGCAC TGCCACAAGA TGCCTTTCAG GGCATTCCGA AGCTCCAACT TCTTGACCTG 
GAGGGCAACG AGATCTCGTA CATACACAAG GAAGCCTTTT CCGGTTTCAC AGCCCTCGAG 
GATCTCAATT TGGGCAACAA TATTTTTCCA GAGTTGCCCG AATCGGGACT GCGCGCTTTG 
CTACACCTAA AGACCTTCAA CAATCCAAAA CTTAGGGAGT TCCCGCCACC GGACACCTTT 
CCCAGAATCC AAACTCTTAT ACTCTCTTAT GCCTACCACT GCTGCGCGTT TCTACCACTG 
GTTGCCATGT CGTCCCAGAA GAAAACCTCC CAGGTCCAGG AGGCTGTACT CTTTCCCTCC 
GATGCCGAGT TCGATATGAC TTTGTGGAAC AATAGCATGA TGAATATTTG GCCGCAAATG 
CATAATCTCA GCAAGCAGTT GGGTGCTTCC ATGCATGATC CTTGGGAGAC GGCTATTAAC 
TTCAACGAAG AGCAGCTGCA AACACAAACG GGAGGCCAAA TCGCCACCAG CTACATGGAG 
GAGTACTTCG AGGAGCACGA TGTGAGTGGT CCTGCCACGG GATACGGCTT TGGTACTGGA 
CTATTCTCTG GTATGTCCAC AGAGGACTTT CAACCGGGTT CGGTGCAGTG CCTGCCAATG 
CCAGGACCTT TTCTACCCTG CGCCGATCTC TTCGATTGGT GGACTCTGCG CTGCGGAGTG 
TGGGTGGTTT TTCTGTTGTC TCTCCTGGGC AACGGAACGG TGGTGTTTGT GCTGCTCTGC 
TCGCGATCAA AGATGGATGT GCCCCGATTT CTAGTCTGTA ATCTGGCCGC GGCCGACTTC 
TTCATGGGCA TCTACCTGGG TATTCTGGCC ATTGTGGATG CGGCGACGTT GGGCGAATTT 
CGCATGTTTG CCATTCCTTG GCAGATGTCC GTGCTCTGCC AGTTGTCCGG TTTTCTGGCC 
GTACTCAGCT CAGAATTATC CGTTTACACG TTGGCTGTGA TCACTTTGGA GCGCAATTAC 
GCCATCACTC ATGCCATTCA CCTGAACAAG AGGCTCTCCT TGAAGCAGGC GGGATATATA 
ATGAGTGTAG GATGGGTTTT TGCCCTGATC ATGGCTTTGA TGCCTTTGGT TGGTGTTTCG 
GACTACAGGA AGTTTGCCGT GTGTTTGCCA TTCGAGACCA CCACTGGACC GGCCAGTTTG 
ACCTATGTAA TCTCACTGAT GTTCATCAAC GGATGCGCAT TTCTCACTCT GATGGGTTGC 
TACCTGAAGA TGTACTGGGC CATAAGGGGC AGCCAGGCAT GGAACACGAA TGATTCGCGA 
ATTGCCAAGC GAATGGCTCT GTTGGTCTTT ACTGACTTCC TCTGCTGGTC ACCTATCGCC 
TTCTTCTCGA TCACTGCCAT CTTTGGCCTG CAACTGATTT CGCTGGAGCA GGCCAAGATC 
TTTACGGTAT TTGTGCTGCC CTTGAATAGT TGCTGCAATC CCTTTCTCTA CGCCATAATG 
ACCAAGCAGT TCAAGAAGGA CTGTGTGACG TTGTGCAAAC ATTTCGAAGA ATCCCGCGTC 
GTGGGAGGCG GTGGTCCGGG TGGACGTGGT GCAGTGGCAA GAACCAAGAG GGGTGATCTC 
CCGCCACCAC TCTTACCAGC TGCAGCGGTG GCACATCCGC CAGGCTGTCG ATGTCTACGG 
ATGCTGCCCA GCGAAATGCC CAACTGGCAT AAAATGGAGC AGACACCGAG CATGTGGCAG 
AGATTGAGAA CATTCTGCTG CGGGGAGAAC CGAAGGCGTC GCAAACAGCG ACGGCAACCG 
CAACAGCGAC GCCAGAGAGC CTATACTGCC GCAGCCGCCA ATCCGTATCA GTATCAGTTT 
GCTGAACTGC GTCAGCAACG CCAGAATCGA GCCAGTTCCA TTTCCAGCGA GAATTTCTGC 
AGCTCAAGAT CATCCAGCTG GCGACATGGT CCACCATCCT CAGCACCCGT GCCACCGGGT 
AACTGCAGCA TGCCGCTGAA GATGCTGGAG CCACATGCTC ATCCGCACGG TCATGGGCGC 
AGACGACACT CCGCCTGGCT GATCACGCGG AAAACGTCGC AGGACTCGAA CTTGTCCAGC 
TCGCGCAATG ACTCCTCCGC CTCGGCCACC ACCGCCAGCA CCTCCACGTT CCGGTTATCC 
CGGTCAAGTG CCGGCAGCAG CACACCCCTG CCCTCGATCA TAGCCCACAA CGGGAAGGCC 
CAGTTGGATG CAGTTAAACC ACGATTGGTC CGCCAGGAAG CAGTCCAGGA GGAAGAGGAC 
TCATCACCAC CTCGCCTGGG AGTACGCTTC CTACCCACCA TACCCTCGGC TGCCGACAGT 
TCTGTCGTCA TGGAAGATGG CGACTCGGCC AACACGGGAG TAGCCAGTTT TCTAGGCATG 
CCACTTCCTG GGGCGTCCAG TGGCTTCCTT ATAGCTCCAA CCACCGCTGC CACCACCTCC 
CCACCCCCGG TGGTCCTGCA GCCGGCAAAG CCTCCTCCCG ATCCCAACGA TGCACCACTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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rk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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