Ance cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ance
Entrez Gene ID	34805
Full Name	Angiotensin converting enzyme
Synonyms	ACE,ANCE,AnCE,BG:DS08220.3,CG8827,Dmel\CG8827,RACE,Race,ance,anon-EST:fe3D10,br31,l(2)34Eb,l(2)br31,l34Eb,race
General protein information	Preferred Names Angiotensin converting enzyme Names CG8827 gene product from transcript CG8827-RA Ance-PA Ance-PB Ance-PC CG8827-PA CG8827-PB CG8827-PC angiotensin I-converting enzyme angiotensin converting enzyme angiotensin-converting enzyme anon-fast-evolving-3D10 race related to angiotensin converting enzyme
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34E2-34E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_165070.3	NP_723852.1	angiotensin converting enzyme, isoform B
NM_057698.5	NP_477046.1	angiotensin converting enzyme, isoform A
NM_001298986.1	NP_001285915.1	angiotensin converting enzyme, isoform C

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ace3	NP_001094923.2
Anopheles gambiae (African malaria mosquito)	ANCE6	XP_318842.4
Drosophila melanogaster (fruit fly)	Ance	NP_723852.1
Anopheles gambiae (African malaria mosquito)	ANCE4	XP_001238056.2
Anopheles gambiae (African malaria mosquito)	ANCE5	XP_001238057.1
Rattus norvegicus (Norway rat)	Ace3	NP_001245166.1
Anopheles gambiae (African malaria mosquito)	ANCE2	XP_001238053.2
Canis lupus familiaris (dog)	LOC100856208	XP_003639303.2
Bos taurus (cattle)	ACE3	XP_005221033.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data report the crystal structures of human testis ACE (tACE) and a homologue of ACE, known as AnCE, from Drosophila melanogaster in complex with a selenium analogue of captopril.
Title: Structural characterization of angiotensin I-converting enzyme in complex with a selenium analogue of captopril.

ANCE, which shares many enzymatic properties with mammalian ACE, is also a product of the male accessory gland of D. melanogaster.
Title: Male accessory glands of Drosophila melanogaster make a secreted angiotensin I-converting enzyme (ANCE), suggesting a role for the peptide-processing enzyme in seminal fluid.

Ance is required for spermatid differentiation.
Title: The drosophila angiotensin-converting enzyme homologue Ance is required for spermiogenesis.

ance may be required for the processing of a developmental peptide hormone or may function in concert with other peptidases to provide amino acids for the synthesis of adult proteins
Title: Ance, a Drosophila angiotensin-converting enzyme homologue, is expressed in imaginal cells during metamorphosis and is regulated by the steroid, 20-hydroxyecdysone.

The following Ance gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ance cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16744	NM_001298986.1 Latest version!	Drosophila melanogaster angiotensin converting enzyme, transcript variant C (Ance), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16744	NM_057698.5 Latest version!	Drosophila melanogaster angiotensin converting enzyme, transcript variant A (Ance), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16744	NM_165070.3 Latest version!	Drosophila melanogaster angiotensin converting enzyme, transcript variant B (Ance), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ance ( NM_165070.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723852.1 Drosophila melanogaster angiotensin converting enzyme, transcript variant B (Ance), mRNA.

Price & Availability↑

CloneID	OFa16744
Clone ID Related Accession (Same CDS sequence)	NM_165070.3 , NM_057698.5 , NM_001298986.1
Accession Version	NM_165070.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	angiotensin converting enzyme, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165070.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGACTGT TTCTGCTAGC CCTGCTGGCT ACTTTGGCGG TAACCCAAGC GCTGGTCAAG 
GAGGAGATAC AGGCCAAGGA GTATCTGGAG AATCTGAACA AGGAGCTGGC CAAGCGAACC 
AACGTGGAAA CCGAAGCTGC CTGGGCCTAT GGCTCCAACA TCACCGACGA GAACGAAAAG 
AAAAAGAATG AGATATCCGC CGAGTTGGCC AAGTTCATGA AGGAGGTGGC CAGTGATACC 
ACCAAGTTCC AATGGCGCTC GTACCAATCG GAGGATCTCA AGCGCCAGTT CAAGGCTCTA 
ACCAAACTGG GCTATGCTGC TCTACCTGAA GACGACTATG CCGAACTGCT GGACACACTC 
TCCGCCATGG AGTCGAATTT CGCCAAGGTC AAGGTGTGCG ACTACAAGGA TAGCACCAAG 
TGCGACCTAG CCCTCGACCC GGAGATCGAG GAGGTCATCA GCAAGAGCCG TGACCACGAG 
GAGCTCGCCT ACTACTGGCG CGAGTTCTAC GACAAGGCCG GAACCGCCGT GCGATCTCAA 
TTCGAACGCT ACGTGGAGCT CAACACCAAG GCAGCCAAAC TGAATAACTT TACTTCTGGT 
GCCGAGGCCT GGTTGGATGA GTACGAGGAC GACACCTTCG AGCAGCAGCT GGAGGACATC 
TTCGCGGATA TTCGTCCGCT TTACCAGCAG ATCCATGGCT ATGTGCGTTT CCGCCTGAGG 
AAACACTATG GTGACGCGGT GGTCTCCGAG ACAGGACCCA TTCCCATGCA CCTATTGGGC 
AACATGTGGG CACAGCAGTG GTCAGAGATT GCGGACATCG TATCCCCCTT TCCGGAGAAG 
CCTCTGGTGG ATGTGAGCGC TGAGATGGAA AAGCAGGGCT ACACGCCACT CAAAATGTTC 
CAAATGGGCG ACGACTTCTT CACGTCTATG AATCTTACCA AGCTGCCACA GGACTTCTGG 
GACAAGTCGA TCATTGAGAA GCCCACCGAT GGTCGTGATC TGGTTTGCCA TGCCAGCGCC 
TGGGACTTTT ATCTCACCGA CGATGTGCGC ATCAAGCAGT GCACCCGGGT TACACAGGAT 
CAGCTCTTCA CCGTTCATCA CGAACTGGGA CACATTCAGT ATTTCCTGCA GTATCAACAC 
CAACCCTTCG TCTATCGCAC TGGTGCCAAT CCCGGTTTCC ACGAAGCTGT CGGTGATGTG 
CTCTCCCTGT CCGTATCGAC ACCCAAACAT CTCGAGAAGA TTGGCCTGTT GAAGGATTAT 
GTGCGCGATG ACGAGGCTCG CATCAACCAG CTCTTCCTCA CCGCCCTGGA TAAGATCGTT 
TTCCTGCCCT TCGCCTTCAC CATGGACAAG TATCGCTGGT CGCTGTTCCG CGGTGAAGTC 
GACAAGGCCA ACTGGAACTG TGCCTTCTGG AAGTTGAGGG ACGAGTACTC CGGCATTGAG 
CCGCCAGTTG TGCGAAGCGA AAAGGACTTT GATGCCCCGG CCAAGTATCA CATTTCCGCC 
GATGTCGAGT ATCTGAGATA CTTGGTCTCC TTCATCATTC AGTTCCAGTT CTACAAGTCC 
GCCTGCATCA AGGCTGGCCA ATATGATCCC GACAATGTGG AGTTGCCTCT GGATAATTGC 
GATATCTACG GAAGTGCTGC AGCTGGTGCT GCTTTCCACA ACATGCTGTC CATGGGCGCC 
TCGAAGCCAT GGCCCGATGC ACTGGAGGCC TTCAACGGCG AGCGCATCAT GTCCGGAAAG 
GCCATCGCCG AATACTTTGA GCCACTGCGC GTTTGGCTGG AGGCGGAGAA CATTAAGAAC 
AATGTCCACA TTGGCTGGAC TACTTCAAAT AAATGCGTCT CATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723852.1
CDS	154..2001
Translation	MRLFLLALLATLAVTQALVKEEIQAKEYLENLNKELAKRTNVETEAAWAYGSNITDENEKKKNEISAELAKFMKEVASDTTKFQWRSYQSEDLKRQFKALTKLGYAALPEDDYAELLDTLSAMESNFAKVKVCDYKDSTKCDLALDPEIEEVISKSRDHEELAYYWREFYDKAGTAVRSQFERYVELNTKAAKLNNFTSGAEAWLDEYEDDTFEQQLEDIFADIRPLYQQIHGYVRFRLRKHYGDAVVSETGPIPMHLLGNMWAQQWSEIADIVSPFPEKPLVDVSAEMEKQGYTPLKMFQMGDDFFTSMNLTKLPQDFWDKSIIEKPTDGRDLVCHASAWDFYLTDDVRIKQCTRVTQDQLFTVHHELGHIQYFLQYQHQPFVYRTGANPGFHEAVGDVLSLSVSTPKHLEKIGLLKDYVRDDEARINQLFLTALDKIVFLPFAFTMDKYRWSLFRGEVDKANWNCAFWKLRDEYSGIEPPVVRSEKDFDAPAKYHISADVEYLRYLVSFIIQFQFYKSACIKAGQYDPDNVELPLDNCDIYGSAAAGAAFHNMLSMGASKPWPDALEAFNGERIMSGKAIAEYFEPLRVWLEAENIKNNVHIGWTTSNKCVSS

Target ORF information:

RefSeq Version	NM_165070.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster angiotensin converting enzyme, transcript variant B (Ance), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165070.3

ORF Insert Sequence:

ATGAGACTGT TTCTGCTAGC CCTGCTGGCT ACTTTGGCGG TAACCCAAGC GCTGGTCAAG 
GAGGAGATAC AGGCCAAGGA GTATCTGGAG AATCTGAACA AGGAGCTGGC CAAGCGAACC 
AACGTGGAAA CCGAAGCTGC CTGGGCCTAT GGCTCCAACA TCACCGACGA GAACGAAAAG 
AAAAAGAATG AGATATCCGC CGAGTTGGCC AAGTTCATGA AGGAGGTGGC CAGTGATACC 
ACCAAGTTCC AATGGCGCTC GTACCAATCG GAGGATCTCA AGCGCCAGTT CAAGGCTCTA 
ACCAAACTGG GCTATGCTGC TCTACCTGAA GACGACTATG CCGAACTGCT GGACACACTC 
TCCGCCATGG AGTCGAATTT CGCCAAGGTC AAGGTGTGCG ACTACAAGGA TAGCACCAAG 
TGCGACCTAG CCCTCGACCC GGAGATCGAG GAGGTCATCA GCAAGAGCCG TGACCACGAG 
GAGCTCGCCT ACTACTGGCG CGAGTTCTAC GACAAGGCCG GAACCGCCGT GCGATCTCAA 
TTCGAACGCT ACGTGGAGCT CAACACCAAG GCAGCCAAAC TGAATAACTT TACTTCTGGT 
GCCGAGGCCT GGTTGGATGA GTACGAGGAC GACACCTTCG AGCAGCAGCT GGAGGACATC 
TTCGCGGATA TTCGTCCGCT TTACCAGCAG ATCCATGGCT ATGTGCGTTT CCGCCTGAGG 
AAACACTATG GTGACGCGGT GGTCTCCGAG ACAGGACCCA TTCCCATGCA CCTATTGGGC 
AACATGTGGG CACAGCAGTG GTCAGAGATT GCGGACATCG TATCCCCCTT TCCGGAGAAG 
CCTCTGGTGG ATGTGAGCGC TGAGATGGAA AAGCAGGGCT ACACGCCACT CAAAATGTTC 
CAAATGGGCG ACGACTTCTT CACGTCTATG AATCTTACCA AGCTGCCACA GGACTTCTGG 
GACAAGTCGA TCATTGAGAA GCCCACCGAT GGTCGTGATC TGGTTTGCCA TGCCAGCGCC 
TGGGACTTTT ATCTCACCGA CGATGTGCGC ATCAAGCAGT GCACCCGGGT TACACAGGAT 
CAGCTCTTCA CCGTTCATCA CGAACTGGGA CACATTCAGT ATTTCCTGCA GTATCAACAC 
CAACCCTTCG TCTATCGCAC TGGTGCCAAT CCCGGTTTCC ACGAAGCTGT CGGTGATGTG 
CTCTCCCTGT CCGTATCGAC ACCCAAACAT CTCGAGAAGA TTGGCCTGTT GAAGGATTAT 
GTGCGCGATG ACGAGGCTCG CATCAACCAG CTCTTCCTCA CCGCCCTGGA TAAGATCGTT 
TTCCTGCCCT TCGCCTTCAC CATGGACAAG TATCGCTGGT CGCTGTTCCG CGGTGAAGTC 
GACAAGGCCA ACTGGAACTG TGCCTTCTGG AAGTTGAGGG ACGAGTACTC CGGCATTGAG 
CCGCCAGTTG TGCGAAGCGA AAAGGACTTT GATGCCCCGG CCAAGTATCA CATTTCCGCC 
GATGTCGAGT ATCTGAGATA CTTGGTCTCC TTCATCATTC AGTTCCAGTT CTACAAGTCC 
GCCTGCATCA AGGCTGGCCA ATATGATCCC GACAATGTGG AGTTGCCTCT GGATAATTGC 
GATATCTACG GAAGTGCTGC AGCTGGTGCT GCTTTCCACA ACATGCTGTC CATGGGCGCC 
TCGAAGCCAT GGCCCGATGC ACTGGAGGCC TTCAACGGCG AGCGCATCAT GTCCGGAAAG 
GCCATCGCCG AATACTTTGA GCCACTGCGC GTTTGGCTGG AGGCGGAGAA CATTAAGAAC 
AATGTCCACA TTGGCTGGAC TACTTCAAAT AAATGCGTCT CATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ance ( NM_057698.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477046.1 Drosophila melanogaster angiotensin converting enzyme, transcript variant A (Ance), mRNA.

Price & Availability↑

CloneID	OFa16744
Clone ID Related Accession (Same CDS sequence)	NM_165070.3 , NM_057698.5 , NM_001298986.1
Accession Version	NM_057698.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	angiotensin converting enzyme, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057698.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGACTGT TTCTGCTAGC CCTGCTGGCT ACTTTGGCGG TAACCCAAGC GCTGGTCAAG 
GAGGAGATAC AGGCCAAGGA GTATCTGGAG AATCTGAACA AGGAGCTGGC CAAGCGAACC 
AACGTGGAAA CCGAAGCTGC CTGGGCCTAT GGCTCCAACA TCACCGACGA GAACGAAAAG 
AAAAAGAATG AGATATCCGC CGAGTTGGCC AAGTTCATGA AGGAGGTGGC CAGTGATACC 
ACCAAGTTCC AATGGCGCTC GTACCAATCG GAGGATCTCA AGCGCCAGTT CAAGGCTCTA 
ACCAAACTGG GCTATGCTGC TCTACCTGAA GACGACTATG CCGAACTGCT GGACACACTC 
TCCGCCATGG AGTCGAATTT CGCCAAGGTC AAGGTGTGCG ACTACAAGGA TAGCACCAAG 
TGCGACCTAG CCCTCGACCC GGAGATCGAG GAGGTCATCA GCAAGAGCCG TGACCACGAG 
GAGCTCGCCT ACTACTGGCG CGAGTTCTAC GACAAGGCCG GAACCGCCGT GCGATCTCAA 
TTCGAACGCT ACGTGGAGCT CAACACCAAG GCAGCCAAAC TGAATAACTT TACTTCTGGT 
GCCGAGGCCT GGTTGGATGA GTACGAGGAC GACACCTTCG AGCAGCAGCT GGAGGACATC 
TTCGCGGATA TTCGTCCGCT TTACCAGCAG ATCCATGGCT ATGTGCGTTT CCGCCTGAGG 
AAACACTATG GTGACGCGGT GGTCTCCGAG ACAGGACCCA TTCCCATGCA CCTATTGGGC 
AACATGTGGG CACAGCAGTG GTCAGAGATT GCGGACATCG TATCCCCCTT TCCGGAGAAG 
CCTCTGGTGG ATGTGAGCGC TGAGATGGAA AAGCAGGGCT ACACGCCACT CAAAATGTTC 
CAAATGGGCG ACGACTTCTT CACGTCTATG AATCTTACCA AGCTGCCACA GGACTTCTGG 
GACAAGTCGA TCATTGAGAA GCCCACCGAT GGTCGTGATC TGGTTTGCCA TGCCAGCGCC 
TGGGACTTTT ATCTCACCGA CGATGTGCGC ATCAAGCAGT GCACCCGGGT TACACAGGAT 
CAGCTCTTCA CCGTTCATCA CGAACTGGGA CACATTCAGT ATTTCCTGCA GTATCAACAC 
CAACCCTTCG TCTATCGCAC TGGTGCCAAT CCCGGTTTCC ACGAAGCTGT CGGTGATGTG 
CTCTCCCTGT CCGTATCGAC ACCCAAACAT CTCGAGAAGA TTGGCCTGTT GAAGGATTAT 
GTGCGCGATG ACGAGGCTCG CATCAACCAG CTCTTCCTCA CCGCCCTGGA TAAGATCGTT 
TTCCTGCCCT TCGCCTTCAC CATGGACAAG TATCGCTGGT CGCTGTTCCG CGGTGAAGTC 
GACAAGGCCA ACTGGAACTG TGCCTTCTGG AAGTTGAGGG ACGAGTACTC CGGCATTGAG 
CCGCCAGTTG TGCGAAGCGA AAAGGACTTT GATGCCCCGG CCAAGTATCA CATTTCCGCC 
GATGTCGAGT ATCTGAGATA CTTGGTCTCC TTCATCATTC AGTTCCAGTT CTACAAGTCC 
GCCTGCATCA AGGCTGGCCA ATATGATCCC GACAATGTGG AGTTGCCTCT GGATAATTGC 
GATATCTACG GAAGTGCTGC AGCTGGTGCT GCTTTCCACA ACATGCTGTC CATGGGCGCC 
TCGAAGCCAT GGCCCGATGC ACTGGAGGCC TTCAACGGCG AGCGCATCAT GTCCGGAAAG 
GCCATCGCCG AATACTTTGA GCCACTGCGC GTTTGGCTGG AGGCGGAGAA CATTAAGAAC 
AATGTCCACA TTGGCTGGAC TACTTCAAAT AAATGCGTCT CATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477046.1
CDS	96..1943
Translation	MRLFLLALLATLAVTQALVKEEIQAKEYLENLNKELAKRTNVETEAAWAYGSNITDENEKKKNEISAELAKFMKEVASDTTKFQWRSYQSEDLKRQFKALTKLGYAALPEDDYAELLDTLSAMESNFAKVKVCDYKDSTKCDLALDPEIEEVISKSRDHEELAYYWREFYDKAGTAVRSQFERYVELNTKAAKLNNFTSGAEAWLDEYEDDTFEQQLEDIFADIRPLYQQIHGYVRFRLRKHYGDAVVSETGPIPMHLLGNMWAQQWSEIADIVSPFPEKPLVDVSAEMEKQGYTPLKMFQMGDDFFTSMNLTKLPQDFWDKSIIEKPTDGRDLVCHASAWDFYLTDDVRIKQCTRVTQDQLFTVHHELGHIQYFLQYQHQPFVYRTGANPGFHEAVGDVLSLSVSTPKHLEKIGLLKDYVRDDEARINQLFLTALDKIVFLPFAFTMDKYRWSLFRGEVDKANWNCAFWKLRDEYSGIEPPVVRSEKDFDAPAKYHISADVEYLRYLVSFIIQFQFYKSACIKAGQYDPDNVELPLDNCDIYGSAAAGAAFHNMLSMGASKPWPDALEAFNGERIMSGKAIAEYFEPLRVWLEAENIKNNVHIGWTTSNKCVSS

Target ORF information:

RefSeq Version	NM_057698.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster angiotensin converting enzyme, transcript variant A (Ance), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057698.5

ORF Insert Sequence:

ATGAGACTGT TTCTGCTAGC CCTGCTGGCT ACTTTGGCGG TAACCCAAGC GCTGGTCAAG 
GAGGAGATAC AGGCCAAGGA GTATCTGGAG AATCTGAACA AGGAGCTGGC CAAGCGAACC 
AACGTGGAAA CCGAAGCTGC CTGGGCCTAT GGCTCCAACA TCACCGACGA GAACGAAAAG 
AAAAAGAATG AGATATCCGC CGAGTTGGCC AAGTTCATGA AGGAGGTGGC CAGTGATACC 
ACCAAGTTCC AATGGCGCTC GTACCAATCG GAGGATCTCA AGCGCCAGTT CAAGGCTCTA 
ACCAAACTGG GCTATGCTGC TCTACCTGAA GACGACTATG CCGAACTGCT GGACACACTC 
TCCGCCATGG AGTCGAATTT CGCCAAGGTC AAGGTGTGCG ACTACAAGGA TAGCACCAAG 
TGCGACCTAG CCCTCGACCC GGAGATCGAG GAGGTCATCA GCAAGAGCCG TGACCACGAG 
GAGCTCGCCT ACTACTGGCG CGAGTTCTAC GACAAGGCCG GAACCGCCGT GCGATCTCAA 
TTCGAACGCT ACGTGGAGCT CAACACCAAG GCAGCCAAAC TGAATAACTT TACTTCTGGT 
GCCGAGGCCT GGTTGGATGA GTACGAGGAC GACACCTTCG AGCAGCAGCT GGAGGACATC 
TTCGCGGATA TTCGTCCGCT TTACCAGCAG ATCCATGGCT ATGTGCGTTT CCGCCTGAGG 
AAACACTATG GTGACGCGGT GGTCTCCGAG ACAGGACCCA TTCCCATGCA CCTATTGGGC 
AACATGTGGG CACAGCAGTG GTCAGAGATT GCGGACATCG TATCCCCCTT TCCGGAGAAG 
CCTCTGGTGG ATGTGAGCGC TGAGATGGAA AAGCAGGGCT ACACGCCACT CAAAATGTTC 
CAAATGGGCG ACGACTTCTT CACGTCTATG AATCTTACCA AGCTGCCACA GGACTTCTGG 
GACAAGTCGA TCATTGAGAA GCCCACCGAT GGTCGTGATC TGGTTTGCCA TGCCAGCGCC 
TGGGACTTTT ATCTCACCGA CGATGTGCGC ATCAAGCAGT GCACCCGGGT TACACAGGAT 
CAGCTCTTCA CCGTTCATCA CGAACTGGGA CACATTCAGT ATTTCCTGCA GTATCAACAC 
CAACCCTTCG TCTATCGCAC TGGTGCCAAT CCCGGTTTCC ACGAAGCTGT CGGTGATGTG 
CTCTCCCTGT CCGTATCGAC ACCCAAACAT CTCGAGAAGA TTGGCCTGTT GAAGGATTAT 
GTGCGCGATG ACGAGGCTCG CATCAACCAG CTCTTCCTCA CCGCCCTGGA TAAGATCGTT 
TTCCTGCCCT TCGCCTTCAC CATGGACAAG TATCGCTGGT CGCTGTTCCG CGGTGAAGTC 
GACAAGGCCA ACTGGAACTG TGCCTTCTGG AAGTTGAGGG ACGAGTACTC CGGCATTGAG 
CCGCCAGTTG TGCGAAGCGA AAAGGACTTT GATGCCCCGG CCAAGTATCA CATTTCCGCC 
GATGTCGAGT ATCTGAGATA CTTGGTCTCC TTCATCATTC AGTTCCAGTT CTACAAGTCC 
GCCTGCATCA AGGCTGGCCA ATATGATCCC GACAATGTGG AGTTGCCTCT GGATAATTGC 
GATATCTACG GAAGTGCTGC AGCTGGTGCT GCTTTCCACA ACATGCTGTC CATGGGCGCC 
TCGAAGCCAT GGCCCGATGC ACTGGAGGCC TTCAACGGCG AGCGCATCAT GTCCGGAAAG 
GCCATCGCCG AATACTTTGA GCCACTGCGC GTTTGGCTGG AGGCGGAGAA CATTAAGAAC 
AATGTCCACA TTGGCTGGAC TACTTCAAAT AAATGCGTCT CATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ance ( NM_001298986.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285915.1 Drosophila melanogaster angiotensin converting enzyme, transcript variant C (Ance), mRNA.

Price & Availability↑

CloneID	OFa16744
Clone ID Related Accession (Same CDS sequence)	NM_165070.3 , NM_057698.5 , NM_001298986.1
Accession Version	NM_001298986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	angiotensin converting enzyme, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGACTGT TTCTGCTAGC CCTGCTGGCT ACTTTGGCGG TAACCCAAGC GCTGGTCAAG 
GAGGAGATAC AGGCCAAGGA GTATCTGGAG AATCTGAACA AGGAGCTGGC CAAGCGAACC 
AACGTGGAAA CCGAAGCTGC CTGGGCCTAT GGCTCCAACA TCACCGACGA GAACGAAAAG 
AAAAAGAATG AGATATCCGC CGAGTTGGCC AAGTTCATGA AGGAGGTGGC CAGTGATACC 
ACCAAGTTCC AATGGCGCTC GTACCAATCG GAGGATCTCA AGCGCCAGTT CAAGGCTCTA 
ACCAAACTGG GCTATGCTGC TCTACCTGAA GACGACTATG CCGAACTGCT GGACACACTC 
TCCGCCATGG AGTCGAATTT CGCCAAGGTC AAGGTGTGCG ACTACAAGGA TAGCACCAAG 
TGCGACCTAG CCCTCGACCC GGAGATCGAG GAGGTCATCA GCAAGAGCCG TGACCACGAG 
GAGCTCGCCT ACTACTGGCG CGAGTTCTAC GACAAGGCCG GAACCGCCGT GCGATCTCAA 
TTCGAACGCT ACGTGGAGCT CAACACCAAG GCAGCCAAAC TGAATAACTT TACTTCTGGT 
GCCGAGGCCT GGTTGGATGA GTACGAGGAC GACACCTTCG AGCAGCAGCT GGAGGACATC 
TTCGCGGATA TTCGTCCGCT TTACCAGCAG ATCCATGGCT ATGTGCGTTT CCGCCTGAGG 
AAACACTATG GTGACGCGGT GGTCTCCGAG ACAGGACCCA TTCCCATGCA CCTATTGGGC 
AACATGTGGG CACAGCAGTG GTCAGAGATT GCGGACATCG TATCCCCCTT TCCGGAGAAG 
CCTCTGGTGG ATGTGAGCGC TGAGATGGAA AAGCAGGGCT ACACGCCACT CAAAATGTTC 
CAAATGGGCG ACGACTTCTT CACGTCTATG AATCTTACCA AGCTGCCACA GGACTTCTGG 
GACAAGTCGA TCATTGAGAA GCCCACCGAT GGTCGTGATC TGGTTTGCCA TGCCAGCGCC 
TGGGACTTTT ATCTCACCGA CGATGTGCGC ATCAAGCAGT GCACCCGGGT TACACAGGAT 
CAGCTCTTCA CCGTTCATCA CGAACTGGGA CACATTCAGT ATTTCCTGCA GTATCAACAC 
CAACCCTTCG TCTATCGCAC TGGTGCCAAT CCCGGTTTCC ACGAAGCTGT CGGTGATGTG 
CTCTCCCTGT CCGTATCGAC ACCCAAACAT CTCGAGAAGA TTGGCCTGTT GAAGGATTAT 
GTGCGCGATG ACGAGGCTCG CATCAACCAG CTCTTCCTCA CCGCCCTGGA TAAGATCGTT 
TTCCTGCCCT TCGCCTTCAC CATGGACAAG TATCGCTGGT CGCTGTTCCG CGGTGAAGTC 
GACAAGGCCA ACTGGAACTG TGCCTTCTGG AAGTTGAGGG ACGAGTACTC CGGCATTGAG 
CCGCCAGTTG TGCGAAGCGA AAAGGACTTT GATGCCCCGG CCAAGTATCA CATTTCCGCC 
GATGTCGAGT ATCTGAGATA CTTGGTCTCC TTCATCATTC AGTTCCAGTT CTACAAGTCC 
GCCTGCATCA AGGCTGGCCA ATATGATCCC GACAATGTGG AGTTGCCTCT GGATAATTGC 
GATATCTACG GAAGTGCTGC AGCTGGTGCT GCTTTCCACA ACATGCTGTC CATGGGCGCC 
TCGAAGCCAT GGCCCGATGC ACTGGAGGCC TTCAACGGCG AGCGCATCAT GTCCGGAAAG 
GCCATCGCCG AATACTTTGA GCCACTGCGC GTTTGGCTGG AGGCGGAGAA CATTAAGAAC 
AATGTCCACA TTGGCTGGAC TACTTCAAAT AAATGCGTCT CATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285915.1
CDS	98..1945
Translation	MRLFLLALLATLAVTQALVKEEIQAKEYLENLNKELAKRTNVETEAAWAYGSNITDENEKKKNEISAELAKFMKEVASDTTKFQWRSYQSEDLKRQFKALTKLGYAALPEDDYAELLDTLSAMESNFAKVKVCDYKDSTKCDLALDPEIEEVISKSRDHEELAYYWREFYDKAGTAVRSQFERYVELNTKAAKLNNFTSGAEAWLDEYEDDTFEQQLEDIFADIRPLYQQIHGYVRFRLRKHYGDAVVSETGPIPMHLLGNMWAQQWSEIADIVSPFPEKPLVDVSAEMEKQGYTPLKMFQMGDDFFTSMNLTKLPQDFWDKSIIEKPTDGRDLVCHASAWDFYLTDDVRIKQCTRVTQDQLFTVHHELGHIQYFLQYQHQPFVYRTGANPGFHEAVGDVLSLSVSTPKHLEKIGLLKDYVRDDEARINQLFLTALDKIVFLPFAFTMDKYRWSLFRGEVDKANWNCAFWKLRDEYSGIEPPVVRSEKDFDAPAKYHISADVEYLRYLVSFIIQFQFYKSACIKAGQYDPDNVELPLDNCDIYGSAAAGAAFHNMLSMGASKPWPDALEAFNGERIMSGKAIAEYFEPLRVWLEAENIKNNVHIGWTTSNKCVSS

Target ORF information:

RefSeq Version	NM_001298986.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster angiotensin converting enzyme, transcript variant C (Ance), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298986.1

ORF Insert Sequence:

ATGAGACTGT TTCTGCTAGC CCTGCTGGCT ACTTTGGCGG TAACCCAAGC GCTGGTCAAG 
GAGGAGATAC AGGCCAAGGA GTATCTGGAG AATCTGAACA AGGAGCTGGC CAAGCGAACC 
AACGTGGAAA CCGAAGCTGC CTGGGCCTAT GGCTCCAACA TCACCGACGA GAACGAAAAG 
AAAAAGAATG AGATATCCGC CGAGTTGGCC AAGTTCATGA AGGAGGTGGC CAGTGATACC 
ACCAAGTTCC AATGGCGCTC GTACCAATCG GAGGATCTCA AGCGCCAGTT CAAGGCTCTA 
ACCAAACTGG GCTATGCTGC TCTACCTGAA GACGACTATG CCGAACTGCT GGACACACTC 
TCCGCCATGG AGTCGAATTT CGCCAAGGTC AAGGTGTGCG ACTACAAGGA TAGCACCAAG 
TGCGACCTAG CCCTCGACCC GGAGATCGAG GAGGTCATCA GCAAGAGCCG TGACCACGAG 
GAGCTCGCCT ACTACTGGCG CGAGTTCTAC GACAAGGCCG GAACCGCCGT GCGATCTCAA 
TTCGAACGCT ACGTGGAGCT CAACACCAAG GCAGCCAAAC TGAATAACTT TACTTCTGGT 
GCCGAGGCCT GGTTGGATGA GTACGAGGAC GACACCTTCG AGCAGCAGCT GGAGGACATC 
TTCGCGGATA TTCGTCCGCT TTACCAGCAG ATCCATGGCT ATGTGCGTTT CCGCCTGAGG 
AAACACTATG GTGACGCGGT GGTCTCCGAG ACAGGACCCA TTCCCATGCA CCTATTGGGC 
AACATGTGGG CACAGCAGTG GTCAGAGATT GCGGACATCG TATCCCCCTT TCCGGAGAAG 
CCTCTGGTGG ATGTGAGCGC TGAGATGGAA AAGCAGGGCT ACACGCCACT CAAAATGTTC 
CAAATGGGCG ACGACTTCTT CACGTCTATG AATCTTACCA AGCTGCCACA GGACTTCTGG 
GACAAGTCGA TCATTGAGAA GCCCACCGAT GGTCGTGATC TGGTTTGCCA TGCCAGCGCC 
TGGGACTTTT ATCTCACCGA CGATGTGCGC ATCAAGCAGT GCACCCGGGT TACACAGGAT 
CAGCTCTTCA CCGTTCATCA CGAACTGGGA CACATTCAGT ATTTCCTGCA GTATCAACAC 
CAACCCTTCG TCTATCGCAC TGGTGCCAAT CCCGGTTTCC ACGAAGCTGT CGGTGATGTG 
CTCTCCCTGT CCGTATCGAC ACCCAAACAT CTCGAGAAGA TTGGCCTGTT GAAGGATTAT 
GTGCGCGATG ACGAGGCTCG CATCAACCAG CTCTTCCTCA CCGCCCTGGA TAAGATCGTT 
TTCCTGCCCT TCGCCTTCAC CATGGACAAG TATCGCTGGT CGCTGTTCCG CGGTGAAGTC 
GACAAGGCCA ACTGGAACTG TGCCTTCTGG AAGTTGAGGG ACGAGTACTC CGGCATTGAG 
CCGCCAGTTG TGCGAAGCGA AAAGGACTTT GATGCCCCGG CCAAGTATCA CATTTCCGCC 
GATGTCGAGT ATCTGAGATA CTTGGTCTCC TTCATCATTC AGTTCCAGTT CTACAAGTCC 
GCCTGCATCA AGGCTGGCCA ATATGATCCC GACAATGTGG AGTTGCCTCT GGATAATTGC 
GATATCTACG GAAGTGCTGC AGCTGGTGCT GCTTTCCACA ACATGCTGTC CATGGGCGCC 
TCGAAGCCAT GGCCCGATGC ACTGGAGGCC TTCAACGGCG AGCGCATCAT GTCCGGAAAG 
GCCATCGCCG AATACTTTGA GCCACTGCGC GTTTGGCTGG AGGCGGAGAA CATTAAGAAC 
AATGTCCACA TTGGCTGGAC TACTTCAAAT AAATGCGTCT CATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ance cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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