b cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	b
Entrez Gene ID	34791
Full Name	black
Synonyms	ADC,B,BG:DS00941.5,CG7811,DGad2,DmGad2,Dmel\CG7811,GAD[[2]],Gad2,anon-34Db,cDNA 1
General protein information	Preferred Names black Names CG7811 gene product from transcript CG7811-RB Aspartate-alpha-decarboxylase CG7811-PA CG7811-PB CG7811-PC Oregon-R glutamate decarboxylase aspartate 1-decarboxylase b-PA b-PB b-PC glutamic acid decarboxylase 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34D1-34D1\|2-48.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298981.1	NP_001285910.1	black, isoform C
NM_057440.5	NP_476788.1	black, isoform A
NM_001259096.2	NP_001246025.1	black, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GADL1	XP_542748.4
Pan troglodytes (chimpanzee)	GADL1	XP_526160.2
	GADL1	XP_001094833.2
Mus musculus (house mouse)	Gadl1	NP_082914.1
Gallus gallus (chicken)	GADL1	XP_003640783.2
Xenopus tropicalis (tropical clawed frog)	gadl1	NP_001039075.1
Homo sapiens (human)	GADL1	NP_997242.2
Bos taurus (cattle)	GADL1	NP_001095751.2
Rattus norvegicus (Norway rat)	Gadl1	XP_006244092.1
Drosophila melanogaster (fruit fly)	b	NP_001246025.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008904	XP_319650.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

6 gamma-ray- and one neutron-induced black mutants displayed the small deletions/insertions and transversion (G --> T) which led to the stop-codon in one case
Title: [Radiation biology of structurally different drosophila genes. Report IV. The black gene: sequencing of the "point" mutations and recombination mechanisms of their processing].

Black expression is strictly accompanied by Ebony expression in optic lobes.
Title: Activity and coexpression of Drosophila black with ebony in fly optic lobes reveals putative cooperative tasks in vision that evade electroretinographic detection.

The presence of cysteine inhibited ADC, CSAD and GDC activity.
Title: Mechanism of cysteine-dependent inactivation of aspartate/glutamate/cysteine sulfinic acid ?-decarboxylases.

Results show that flies with black/DGAD2 mutation are reduced in aspartate decarboxylase activity, and respond abnormally to visual cues.
Title: The Drosophila black enigma: the molecular and behavioural characterization of the black1 mutant allele.

The following b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14684	NM_057440.5 Latest version!	Drosophila melanogaster black (b), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14684	NM_001259096.2 Latest version!	Drosophila melanogaster black (b), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa21649	NM_001298981.1 Latest version!	Drosophila melanogaster black (b), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

b ( NM_057440.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476788.1 Drosophila melanogaster black (b), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14684
Clone ID Related Accession (Same CDS sequence)	NM_057440.5 , NM_001259096.2
Accession Version	NM_057440.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	black, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057440.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGGCCA GCGAGAACTT TCCCACACAT CACTTCAAGG AATCTATATT TAAGCCGTAC 
AGTACGACGA GTGGCGATGA TTTGGCAAGC GTTAGCCCAT TGACAGCGAC GGCAGCGTTG 
GTGGCGTCCA CGTCGTCACC GGCTGATTCC ACCTCCACAG TTGCATTCGA GCAGGCCAGC 
AAAATGTTGG CCAACGCCGC CAACAATAAT AATAATAATA ATAACAACAT CACGAGCACC 
AAGGATGATC TATCCAGCTT TGTGGCCAGC CATCCGGCGG CAGAGTTCGA GGGCTTCATC 
CGCGCTTGCG TCGACGAGAT CATCAAGCTG GCCGTCTTCC AGGGCACCAA TCGCTCCTCG 
AAGGTCGTCG AGTGGCACGA GCCAGCTGAG CTGCGACAGC TCTTTGACTT CCAGCTGCGG 
GAGCAGGGCG AATCGCAGGA CAAGCTGCGC GAGCTGCTGC GGGAAACGAT TCGATTCTCC 
GTGAAGACAG GACATCCGTA CTTCATCAAT CAGTTGTACT CGGGTGTGGA TCCATACGCG 
CTGGTAGGTC AATGGCTCAC CGATGCCCTC AATCCCAGCG TGTACACGTA CGAGGTGGCT 
CCACTCTTTA CGCTGATGGA GGAGCAGGTG TTGGCCGAGA TGCGACGCAT TGTGGGCTTC 
CCCAATGGCG GCCAGGGTGA TGGCATCTTC TGTCCCGGCG GCTCGATAGC CAACGGTTAT 
GCAATCAGCT GCGCCCGCTA CAGACACTCG CCCGAGTCCA AGAAAAACGG ACTCTTTAAT 
GCCAAGCCGC TGATTATCTT CACCTCGGAG GACGCGCACT ACTCCGTGGA GAAGCTGGCC 
ATGTTCATGG GCTTCGGCAG CGATCATGTG CGCAAGATAG CCACCAACGA GGTGGGCAAG 
ATGCGGCTGA GCGACCTGGA GAAACAGGTG AAGCTGTGCC TGGAGAACGG CTGGCAGCCG 
CTGATGGTAT CGGCCACAGC GGGTACCACT GTGCTGGGCG CCTTCGATGA TCTGGCTGGA 
ATCTCGGAGG TGTGCAAGAA GTACAACATG TGGATGCACG TGGATGCGGC TTGGGGTGGC 
GGTGCGCTCA TGTCCAAGAA GTATCGCCAT CTGCTCAATG GCATCGAACG GGCTGATTCG 
GTCACTTGGA ATCCACACAA GCTGCTGGCT GCCTCGCAAC AGTGTTCCAC CTTCCTGACG 
CGTCACCAGC AGGTGCTGGC CCAGTGCCAT TCGACCAATG CGACATACTT GTTCCAGAAG 
GACAAGTTCT ATGACACATC CTTCGACACC GGCGACAAGC ACATCCAGTG CGGCCGACGA 
GCGGATGTCT TCAAGTTCTG GTTCATGTGG AAGGCGAAGG GCACCCAGGG TCTGGAGGCG 
CATGTCGAGA AGGTCTTCCG CATGGCCGAG TTCTTCACGG CCAAGGTGAG GGAGCGTCCT 
GGATTCGAGC TCGTCCTCGA GAGTCCCGAG TGCACCAACA TTAGCTTCTG GTATGTGCCG 
CCCGGTCTGC GGGAAATGGA GCGCAATCGC GAGTTCTACG ACCGTCTGCA TAAGGTGGCG 
CCGAAGGTCA AGGAGGGCAT GATCAAGAAG GGCTCCATGA TGATCACGTA CCAGCCGTTG 
CGCCAGCTGC CCAACTTCTT TCGCCTGGTG CTGCAGAACT CCTGCCTGGA GGAGTCGGAC 
ATGGTCTACT TTCTGGACGA GATCGAATCG CTCGCCCAAA ACTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476788.1
CDS	140..1867
Translation	MLASENFPTHHFKESIFKPYSTTSGDDLASVSPLTATAALVASTSSPADSTSTVAFEQASKMLANAANNNNNNNNNITSTKDDLSSFVASHPAAEFEGFIRACVDEIIKLAVFQGTNRSSKVVEWHEPAELRQLFDFQLREQGESQDKLRELLRETIRFSVKTGHPYFINQLYSGVDPYALVGQWLTDALNPSVYTYEVAPLFTLMEEQVLAEMRRIVGFPNGGQGDGIFCPGGSIANGYAISCARYRHSPESKKNGLFNAKPLIIFTSEDAHYSVEKLAMFMGFGSDHVRKIATNEVGKMRLSDLEKQVKLCLENGWQPLMVSATAGTTVLGAFDDLAGISEVCKKYNMWMHVDAAWGGGALMSKKYRHLLNGIERADSVTWNPHKLLAASQQCSTFLTRHQQVLAQCHSTNATYLFQKDKFYDTSFDTGDKHIQCGRRADVFKFWFMWKAKGTQGLEAHVEKVFRMAEFFTAKVRERPGFELVLESPECTNISFWYVPPGLREMERNREFYDRLHKVAPKVKEGMIKKGSMMITYQPLRQLPNFFRLVLQNSCLEESDMVYFLDEIESLAQNL

Target ORF information:

RefSeq Version	NM_057440.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster black (b), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057440.5

ORF Insert Sequence:

ATGCTGGCCA GCGAGAACTT TCCCACACAT CACTTCAAGG AATCTATATT TAAGCCGTAC 
AGTACGACGA GTGGCGATGA TTTGGCAAGC GTTAGCCCAT TGACAGCGAC GGCAGCGTTG 
GTGGCGTCCA CGTCGTCACC GGCTGATTCC ACCTCCACAG TTGCATTCGA GCAGGCCAGC 
AAAATGTTGG CCAACGCCGC CAACAATAAT AATAATAATA ATAACAACAT CACGAGCACC 
AAGGATGATC TATCCAGCTT TGTGGCCAGC CATCCGGCGG CAGAGTTCGA GGGCTTCATC 
CGCGCTTGCG TCGACGAGAT CATCAAGCTG GCCGTCTTCC AGGGCACCAA TCGCTCCTCG 
AAGGTCGTCG AGTGGCACGA GCCAGCTGAG CTGCGACAGC TCTTTGACTT CCAGCTGCGG 
GAGCAGGGCG AATCGCAGGA CAAGCTGCGC GAGCTGCTGC GGGAAACGAT TCGATTCTCC 
GTGAAGACAG GACATCCGTA CTTCATCAAT CAGTTGTACT CGGGTGTGGA TCCATACGCG 
CTGGTAGGTC AATGGCTCAC CGATGCCCTC AATCCCAGCG TGTACACGTA CGAGGTGGCT 
CCACTCTTTA CGCTGATGGA GGAGCAGGTG TTGGCCGAGA TGCGACGCAT TGTGGGCTTC 
CCCAATGGCG GCCAGGGTGA TGGCATCTTC TGTCCCGGCG GCTCGATAGC CAACGGTTAT 
GCAATCAGCT GCGCCCGCTA CAGACACTCG CCCGAGTCCA AGAAAAACGG ACTCTTTAAT 
GCCAAGCCGC TGATTATCTT CACCTCGGAG GACGCGCACT ACTCCGTGGA GAAGCTGGCC 
ATGTTCATGG GCTTCGGCAG CGATCATGTG CGCAAGATAG CCACCAACGA GGTGGGCAAG 
ATGCGGCTGA GCGACCTGGA GAAACAGGTG AAGCTGTGCC TGGAGAACGG CTGGCAGCCG 
CTGATGGTAT CGGCCACAGC GGGTACCACT GTGCTGGGCG CCTTCGATGA TCTGGCTGGA 
ATCTCGGAGG TGTGCAAGAA GTACAACATG TGGATGCACG TGGATGCGGC TTGGGGTGGC 
GGTGCGCTCA TGTCCAAGAA GTATCGCCAT CTGCTCAATG GCATCGAACG GGCTGATTCG 
GTCACTTGGA ATCCACACAA GCTGCTGGCT GCCTCGCAAC AGTGTTCCAC CTTCCTGACG 
CGTCACCAGC AGGTGCTGGC CCAGTGCCAT TCGACCAATG CGACATACTT GTTCCAGAAG 
GACAAGTTCT ATGACACATC CTTCGACACC GGCGACAAGC ACATCCAGTG CGGCCGACGA 
GCGGATGTCT TCAAGTTCTG GTTCATGTGG AAGGCGAAGG GCACCCAGGG TCTGGAGGCG 
CATGTCGAGA AGGTCTTCCG CATGGCCGAG TTCTTCACGG CCAAGGTGAG GGAGCGTCCT 
GGATTCGAGC TCGTCCTCGA GAGTCCCGAG TGCACCAACA TTAGCTTCTG GTATGTGCCG 
CCCGGTCTGC GGGAAATGGA GCGCAATCGC GAGTTCTACG ACCGTCTGCA TAAGGTGGCG 
CCGAAGGTCA AGGAGGGCAT GATCAAGAAG GGCTCCATGA TGATCACGTA CCAGCCGTTG 
CGCCAGCTGC CCAACTTCTT TCGCCTGGTG CTGCAGAACT CCTGCCTGGA GGAGTCGGAC 
ATGGTCTACT TTCTGGACGA GATCGAATCG CTCGCCCAAA ACTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

b ( NM_001259096.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246025.1 Drosophila melanogaster black (b), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14684
Clone ID Related Accession (Same CDS sequence)	NM_057440.5 , NM_001259096.2
Accession Version	NM_001259096.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	black, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259096.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGGCCA GCGAGAACTT TCCCACACAT CACTTCAAGG AATCTATATT TAAGCCGTAC 
AGTACGACGA GTGGCGATGA TTTGGCAAGC GTTAGCCCAT TGACAGCGAC GGCAGCGTTG 
GTGGCGTCCA CGTCGTCACC GGCTGATTCC ACCTCCACAG TTGCATTCGA GCAGGCCAGC 
AAAATGTTGG CCAACGCCGC CAACAATAAT AATAATAATA ATAACAACAT CACGAGCACC 
AAGGATGATC TATCCAGCTT TGTGGCCAGC CATCCGGCGG CAGAGTTCGA GGGCTTCATC 
CGCGCTTGCG TCGACGAGAT CATCAAGCTG GCCGTCTTCC AGGGCACCAA TCGCTCCTCG 
AAGGTCGTCG AGTGGCACGA GCCAGCTGAG CTGCGACAGC TCTTTGACTT CCAGCTGCGG 
GAGCAGGGCG AATCGCAGGA CAAGCTGCGC GAGCTGCTGC GGGAAACGAT TCGATTCTCC 
GTGAAGACAG GACATCCGTA CTTCATCAAT CAGTTGTACT CGGGTGTGGA TCCATACGCG 
CTGGTAGGTC AATGGCTCAC CGATGCCCTC AATCCCAGCG TGTACACGTA CGAGGTGGCT 
CCACTCTTTA CGCTGATGGA GGAGCAGGTG TTGGCCGAGA TGCGACGCAT TGTGGGCTTC 
CCCAATGGCG GCCAGGGTGA TGGCATCTTC TGTCCCGGCG GCTCGATAGC CAACGGTTAT 
GCAATCAGCT GCGCCCGCTA CAGACACTCG CCCGAGTCCA AGAAAAACGG ACTCTTTAAT 
GCCAAGCCGC TGATTATCTT CACCTCGGAG GACGCGCACT ACTCCGTGGA GAAGCTGGCC 
ATGTTCATGG GCTTCGGCAG CGATCATGTG CGCAAGATAG CCACCAACGA GGTGGGCAAG 
ATGCGGCTGA GCGACCTGGA GAAACAGGTG AAGCTGTGCC TGGAGAACGG CTGGCAGCCG 
CTGATGGTAT CGGCCACAGC GGGTACCACT GTGCTGGGCG CCTTCGATGA TCTGGCTGGA 
ATCTCGGAGG TGTGCAAGAA GTACAACATG TGGATGCACG TGGATGCGGC TTGGGGTGGC 
GGTGCGCTCA TGTCCAAGAA GTATCGCCAT CTGCTCAATG GCATCGAACG GGCTGATTCG 
GTCACTTGGA ATCCACACAA GCTGCTGGCT GCCTCGCAAC AGTGTTCCAC CTTCCTGACG 
CGTCACCAGC AGGTGCTGGC CCAGTGCCAT TCGACCAATG CGACATACTT GTTCCAGAAG 
GACAAGTTCT ATGACACATC CTTCGACACC GGCGACAAGC ACATCCAGTG CGGCCGACGA 
GCGGATGTCT TCAAGTTCTG GTTCATGTGG AAGGCGAAGG GCACCCAGGG TCTGGAGGCG 
CATGTCGAGA AGGTCTTCCG CATGGCCGAG TTCTTCACGG CCAAGGTGAG GGAGCGTCCT 
GGATTCGAGC TCGTCCTCGA GAGTCCCGAG TGCACCAACA TTAGCTTCTG GTATGTGCCG 
CCCGGTCTGC GGGAAATGGA GCGCAATCGC GAGTTCTACG ACCGTCTGCA TAAGGTGGCG 
CCGAAGGTCA AGGAGGGCAT GATCAAGAAG GGCTCCATGA TGATCACGTA CCAGCCGTTG 
CGCCAGCTGC CCAACTTCTT TCGCCTGGTG CTGCAGAACT CCTGCCTGGA GGAGTCGGAC 
ATGGTCTACT TTCTGGACGA GATCGAATCG CTCGCCCAAA ACTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246025.1
CDS	140..1867
Translation	MLASENFPTHHFKESIFKPYSTTSGDDLASVSPLTATAALVASTSSPADSTSTVAFEQASKMLANAANNNNNNNNNITSTKDDLSSFVASHPAAEFEGFIRACVDEIIKLAVFQGTNRSSKVVEWHEPAELRQLFDFQLREQGESQDKLRELLRETIRFSVKTGHPYFINQLYSGVDPYALVGQWLTDALNPSVYTYEVAPLFTLMEEQVLAEMRRIVGFPNGGQGDGIFCPGGSIANGYAISCARYRHSPESKKNGLFNAKPLIIFTSEDAHYSVEKLAMFMGFGSDHVRKIATNEVGKMRLSDLEKQVKLCLENGWQPLMVSATAGTTVLGAFDDLAGISEVCKKYNMWMHVDAAWGGGALMSKKYRHLLNGIERADSVTWNPHKLLAASQQCSTFLTRHQQVLAQCHSTNATYLFQKDKFYDTSFDTGDKHIQCGRRADVFKFWFMWKAKGTQGLEAHVEKVFRMAEFFTAKVRERPGFELVLESPECTNISFWYVPPGLREMERNREFYDRLHKVAPKVKEGMIKKGSMMITYQPLRQLPNFFRLVLQNSCLEESDMVYFLDEIESLAQNL

Target ORF information:

RefSeq Version	NM_001259096.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster black (b), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259096.2

ORF Insert Sequence:

ATGCTGGCCA GCGAGAACTT TCCCACACAT CACTTCAAGG AATCTATATT TAAGCCGTAC 
AGTACGACGA GTGGCGATGA TTTGGCAAGC GTTAGCCCAT TGACAGCGAC GGCAGCGTTG 
GTGGCGTCCA CGTCGTCACC GGCTGATTCC ACCTCCACAG TTGCATTCGA GCAGGCCAGC 
AAAATGTTGG CCAACGCCGC CAACAATAAT AATAATAATA ATAACAACAT CACGAGCACC 
AAGGATGATC TATCCAGCTT TGTGGCCAGC CATCCGGCGG CAGAGTTCGA GGGCTTCATC 
CGCGCTTGCG TCGACGAGAT CATCAAGCTG GCCGTCTTCC AGGGCACCAA TCGCTCCTCG 
AAGGTCGTCG AGTGGCACGA GCCAGCTGAG CTGCGACAGC TCTTTGACTT CCAGCTGCGG 
GAGCAGGGCG AATCGCAGGA CAAGCTGCGC GAGCTGCTGC GGGAAACGAT TCGATTCTCC 
GTGAAGACAG GACATCCGTA CTTCATCAAT CAGTTGTACT CGGGTGTGGA TCCATACGCG 
CTGGTAGGTC AATGGCTCAC CGATGCCCTC AATCCCAGCG TGTACACGTA CGAGGTGGCT 
CCACTCTTTA CGCTGATGGA GGAGCAGGTG TTGGCCGAGA TGCGACGCAT TGTGGGCTTC 
CCCAATGGCG GCCAGGGTGA TGGCATCTTC TGTCCCGGCG GCTCGATAGC CAACGGTTAT 
GCAATCAGCT GCGCCCGCTA CAGACACTCG CCCGAGTCCA AGAAAAACGG ACTCTTTAAT 
GCCAAGCCGC TGATTATCTT CACCTCGGAG GACGCGCACT ACTCCGTGGA GAAGCTGGCC 
ATGTTCATGG GCTTCGGCAG CGATCATGTG CGCAAGATAG CCACCAACGA GGTGGGCAAG 
ATGCGGCTGA GCGACCTGGA GAAACAGGTG AAGCTGTGCC TGGAGAACGG CTGGCAGCCG 
CTGATGGTAT CGGCCACAGC GGGTACCACT GTGCTGGGCG CCTTCGATGA TCTGGCTGGA 
ATCTCGGAGG TGTGCAAGAA GTACAACATG TGGATGCACG TGGATGCGGC TTGGGGTGGC 
GGTGCGCTCA TGTCCAAGAA GTATCGCCAT CTGCTCAATG GCATCGAACG GGCTGATTCG 
GTCACTTGGA ATCCACACAA GCTGCTGGCT GCCTCGCAAC AGTGTTCCAC CTTCCTGACG 
CGTCACCAGC AGGTGCTGGC CCAGTGCCAT TCGACCAATG CGACATACTT GTTCCAGAAG 
GACAAGTTCT ATGACACATC CTTCGACACC GGCGACAAGC ACATCCAGTG CGGCCGACGA 
GCGGATGTCT TCAAGTTCTG GTTCATGTGG AAGGCGAAGG GCACCCAGGG TCTGGAGGCG 
CATGTCGAGA AGGTCTTCCG CATGGCCGAG TTCTTCACGG CCAAGGTGAG GGAGCGTCCT 
GGATTCGAGC TCGTCCTCGA GAGTCCCGAG TGCACCAACA TTAGCTTCTG GTATGTGCCG 
CCCGGTCTGC GGGAAATGGA GCGCAATCGC GAGTTCTACG ACCGTCTGCA TAAGGTGGCG 
CCGAAGGTCA AGGAGGGCAT GATCAAGAAG GGCTCCATGA TGATCACGTA CCAGCCGTTG 
CGCCAGCTGC CCAACTTCTT TCGCCTGGTG CTGCAGAACT CCTGCCTGGA GGAGTCGGAC 
ATGGTCTACT TTCTGGACGA GATCGAATCG CTCGCCCAAA ACTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

b ( NM_001298981.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285910.1 Drosophila melanogaster black (b), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa21649
Clone ID Related Accession (Same CDS sequence)	NM_001298981.1
Accession Version	NM_001298981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	black, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGGCCA ACGCCGCCAA CAATAATAAT AATAATAATA ACAACATCAC GAGCACCAAG 
GATGATCTAT CCAGCTTTGT GGCCAGCCAT CCGGCGGCAG AGTTCGAGGG CTTCATCCGC 
GCTTGCGTCG ACGAGATCAT CAAGCTGGCC GTCTTCCAGG GCACCAATCG CTCCTCGAAG 
GTCGTCGAGT GGCACGAGCC AGCTGAGCTG CGACAGCTCT TTGACTTCCA GCTGCGGGAG 
CAGGGCGAAT CGCAGGACAA GCTGCGCGAG CTGCTGCGGG AAACGATTCG ATTCTCCGTG 
AAGACAGGAC ATCCGTACTT CATCAATCAG TTGTACTCGG GTGTGGATCC ATACGCGCTG 
GTAGGTCAAT GGCTCACCGA TGCCCTCAAT CCCAGCGTGT ACACGTACGA GGTGGCTCCA 
CTCTTTACGC TGATGGAGGA GCAGGTGTTG GCCGAGATGC GACGCATTGT GGGCTTCCCC 
AATGGCGGCC AGGGTGATGG CATCTTCTGT CCCGGCGGCT CGATAGCCAA CGGTTATGCA 
ATCAGCTGCG CCCGCTACAG ACACTCGCCC GAGTCCAAGA AAAACGGACT CTTTAATGCC 
AAGCCGCTGA TTATCTTCAC CTCGGAGGAC GCGCACTACT CCGTGGAGAA GCTGGCCATG 
TTCATGGGCT TCGGCAGCGA TCATGTGCGC AAGATAGCCA CCAACGAGGT GGGCAAGATG 
CGGCTGAGCG ACCTGGAGAA ACAGGTGAAG CTGTGCCTGG AGAACGGCTG GCAGCCGCTG 
ATGGTATCGG CCACAGCGGG TACCACTGTG CTGGGCGCCT TCGATGATCT GGCTGGAATC 
TCGGAGGTGT GCAAGAAGTA CAACATGTGG ATGCACGTGG ATGCGGCTTG GGGTGGCGGT 
GCGCTCATGT CCAAGAAGTA TCGCCATCTG CTCAATGGCA TCGAACGGGC TGATTCGGTC 
ACTTGGAATC CACACAAGCT GCTGGCTGCC TCGCAACAGT GTTCCACCTT CCTGACGCGT 
CACCAGCAGG TGCTGGCCCA GTGCCATTCG ACCAATGCGA CATACTTGTT CCAGAAGGAC 
AAGTTCTATG ACACATCCTT CGACACCGGC GACAAGCACA TCCAGTGCGG CCGACGAGCG 
GATGTCTTCA AGTTCTGGTT CATGTGGAAG GCGAAGGGCA CCCAGGGTCT GGAGGCGCAT 
GTCGAGAAGG TCTTCCGCAT GGCCGAGTTC TTCACGGCCA AGGTGAGGGA GCGTCCTGGA 
TTCGAGCTCG TCCTCGAGAG TCCCGAGTGC ACCAACATTA GCTTCTGGTA TGTGCCGCCC 
GGTCTGCGGG AAATGGAGCG CAATCGCGAG TTCTACGACC GTCTGCATAA GGTGGCGCCG 
AAGGTCAAGG AGGGCATGAT CAAGAAGGGC TCCATGATGA TCACGTACCA GCCGTTGCGC 
CAGCTGCCCA ACTTCTTTCG CCTGGTGCTG CAGAACTCCT GCCTGGAGGA GTCGGACATG 
GTCTACTTTC TGGACGAGAT CGAATCGCTC GCCCAAAACT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285910.1
CDS	323..1867
Translation	MLANAANNNNNNNNNITSTKDDLSSFVASHPAAEFEGFIRACVDEIIKLAVFQGTNRSSKVVEWHEPAELRQLFDFQLREQGESQDKLRELLRETIRFSVKTGHPYFINQLYSGVDPYALVGQWLTDALNPSVYTYEVAPLFTLMEEQVLAEMRRIVGFPNGGQGDGIFCPGGSIANGYAISCARYRHSPESKKNGLFNAKPLIIFTSEDAHYSVEKLAMFMGFGSDHVRKIATNEVGKMRLSDLEKQVKLCLENGWQPLMVSATAGTTVLGAFDDLAGISEVCKKYNMWMHVDAAWGGGALMSKKYRHLLNGIERADSVTWNPHKLLAASQQCSTFLTRHQQVLAQCHSTNATYLFQKDKFYDTSFDTGDKHIQCGRRADVFKFWFMWKAKGTQGLEAHVEKVFRMAEFFTAKVRERPGFELVLESPECTNISFWYVPPGLREMERNREFYDRLHKVAPKVKEGMIKKGSMMITYQPLRQLPNFFRLVLQNSCLEESDMVYFLDEIESLAQNL

Target ORF information:

RefSeq Version	NM_001298981.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster black (b), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298981.1

ORF Insert Sequence:

ATGTTGGCCA ACGCCGCCAA CAATAATAAT AATAATAATA ACAACATCAC GAGCACCAAG 
GATGATCTAT CCAGCTTTGT GGCCAGCCAT CCGGCGGCAG AGTTCGAGGG CTTCATCCGC 
GCTTGCGTCG ACGAGATCAT CAAGCTGGCC GTCTTCCAGG GCACCAATCG CTCCTCGAAG 
GTCGTCGAGT GGCACGAGCC AGCTGAGCTG CGACAGCTCT TTGACTTCCA GCTGCGGGAG 
CAGGGCGAAT CGCAGGACAA GCTGCGCGAG CTGCTGCGGG AAACGATTCG ATTCTCCGTG 
AAGACAGGAC ATCCGTACTT CATCAATCAG TTGTACTCGG GTGTGGATCC ATACGCGCTG 
GTAGGTCAAT GGCTCACCGA TGCCCTCAAT CCCAGCGTGT ACACGTACGA GGTGGCTCCA 
CTCTTTACGC TGATGGAGGA GCAGGTGTTG GCCGAGATGC GACGCATTGT GGGCTTCCCC 
AATGGCGGCC AGGGTGATGG CATCTTCTGT CCCGGCGGCT CGATAGCCAA CGGTTATGCA 
ATCAGCTGCG CCCGCTACAG ACACTCGCCC GAGTCCAAGA AAAACGGACT CTTTAATGCC 
AAGCCGCTGA TTATCTTCAC CTCGGAGGAC GCGCACTACT CCGTGGAGAA GCTGGCCATG 
TTCATGGGCT TCGGCAGCGA TCATGTGCGC AAGATAGCCA CCAACGAGGT GGGCAAGATG 
CGGCTGAGCG ACCTGGAGAA ACAGGTGAAG CTGTGCCTGG AGAACGGCTG GCAGCCGCTG 
ATGGTATCGG CCACAGCGGG TACCACTGTG CTGGGCGCCT TCGATGATCT GGCTGGAATC 
TCGGAGGTGT GCAAGAAGTA CAACATGTGG ATGCACGTGG ATGCGGCTTG GGGTGGCGGT 
GCGCTCATGT CCAAGAAGTA TCGCCATCTG CTCAATGGCA TCGAACGGGC TGATTCGGTC 
ACTTGGAATC CACACAAGCT GCTGGCTGCC TCGCAACAGT GTTCCACCTT CCTGACGCGT 
CACCAGCAGG TGCTGGCCCA GTGCCATTCG ACCAATGCGA CATACTTGTT CCAGAAGGAC 
AAGTTCTATG ACACATCCTT CGACACCGGC GACAAGCACA TCCAGTGCGG CCGACGAGCG 
GATGTCTTCA AGTTCTGGTT CATGTGGAAG GCGAAGGGCA CCCAGGGTCT GGAGGCGCAT 
GTCGAGAAGG TCTTCCGCAT GGCCGAGTTC TTCACGGCCA AGGTGAGGGA GCGTCCTGGA 
TTCGAGCTCG TCCTCGAGAG TCCCGAGTGC ACCAACATTA GCTTCTGGTA TGTGCCGCCC 
GGTCTGCGGG AAATGGAGCG CAATCGCGAG TTCTACGACC GTCTGCATAA GGTGGCGCCG 
AAGGTCAAGG AGGGCATGAT CAAGAAGGGC TCCATGATGA TCACGTACCA GCCGTTGCGC 
CAGCTGCCCA ACTTCTTTCG CCTGGTGCTG CAGAACTCCT GCCTGGAGGA GTCGGACATG 
GTCTACTTTC TGGACGAGAT CGAATCGCTC GCCCAAAACT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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b cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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