CG31729 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG31729
Entrez Gene ID	34736
Full Name	CG31729 gene product from transcript CG31729-RB
Synonyms	CG16823,CG6263,DmCG31729,Dmel\CG31729,P4-ATPase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34B4-34B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_135790.4	NP_609634.1	uncharacterized protein, isoform B
NM_001298957.1	NP_001285886.1	uncharacterized protein, isoform C
NM_165038.3	NP_723806.1	uncharacterized protein, isoform A
NM_001298958.1	NP_001285887.1	uncharacterized protein, isoform D

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	atp9b	NP_001038619.1
Kluyveromyces lactis	KLLA0C08393g	XP_452573.1
Magnaporthe oryzae (rice blast fungus)	MGG_04066	XP_003719773.1
Neurospora crassa	NCU03818	XP_961207.1
Homo sapiens (human)	ATP9B	NP_940933.3
Bos taurus (cattle)	ATP9B	NP_001073724.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008184	XP_317283.4
Caenorhabditis elegans (roundworm)	tat-6	NP_503858.1
Eremothecium gossypii	AGOS_ADL079C	NP_984017.1
Rattus norvegicus (Norway rat)	Atp9b	NP_001099600.1
Drosophila melanogaster (fruit fly)	CG31729	NP_723806.1
Caenorhabditis elegans (roundworm)	tat-5	NP_001040665.1
Xenopus tropicalis (tropical clawed frog)	atp9b	XP_002936489.2
Pan troglodytes (chimpanzee)	ATP9B	XP_001143568.1
	ATP9B	XP_001087804.1
Mus musculus (house mouse)	Atp9b	NP_001188498.1
Gallus gallus (chicken)	ATP9B	XP_003640794.1
Canis lupus familiaris (dog)	ATP9B	XP_005615350.1
Saccharomyces cerevisiae (baker's yeast)	NEO1	NP_012216.1
Schizosaccharomyces pombe (fission yeast)	SPAC6C3.06c	NP_593720.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG31729 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG31729 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa21640	NM_001298958.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG31729), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa14637	NM_165038.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31729), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21641	NM_001298957.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG31729), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa14636	NM_135790.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG31729), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG31729 ( NM_001298958.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285887.1 Drosophila melanogaster uncharacterized protein, transcript variant D (CG31729), mRNA.

Price & Availability↑

CloneID	OFa21640
Clone ID Related Accession (Same CDS sequence)	NM_001298958.1
Accession Version	NM_001298958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTGT CGCAGTTCAT TCCGGACATC CGGATCGGTT ACCCGTACAC CTACTGGGGT 
CCGCTCGGCT TCGTGCTAAT GGTCACCATT TGCCGAGAGG CGGTCGACGA TCTGCGTCGG 
CATCAGCGGG ATCACGAGGT AAACTCGCAG AAGTACAAGA GACTGTCGTC CACCAACATC 
AGCGGCTACG AAATGGTTCC CAGCTCCAAG CTGAAGGTCG GTGACGTCAT AATCGTGGAA 
AAGAACGAGC GCGTGCCAGC GGATCTGATC CTGCTGCGCA CCAGCGATCG GAGCGGATCG 
GTCTTTGTTC GTACGGACCA ACTTGATGGC GAGACGGACT GGAAACCACG TCTCGCCGTT 
CCGTATACTC AAAAACTCAG TCGAGATTCG GAGCTACATA GCATCGATGC CAGTTTTTAC 
GTGGAGAAGC CACAGAACGA CATTCACAGC TTCATCGCCA CCTTCTGCAT GGCAGACGGC 
AGCGAAGACA CCGGTCTCAG TGTGGAGAAT ACCCTGTGGG CCAACACCGT CGTAGCCGCA 
GGAACAGCCA CAGGAATTGT CATATATACT GGCTGCGAAA CTCGCAGCGT GATGAATAAC 
TCACAGCCAA GGTCCAAAGT TGGACTGCTA GACATGGAGA TTAACGGATT GACTAAGGTT 
CTGTTCTGCG CCGTGCTTGG ACTGTCGCTA GTCATGATGA TGCTTAAGGG TTTCGGCGGT 
CCTTGGTATA GATACATGTT CCGCTTCGTT CTTCTCTTCT CCTACATCAT TCCAATCAGT 
CTGCGTGTCA ATTTGGACAT GGGAAAGGCG TTCTACTCAT GGCAAATGCA AAACGATTCG 
AACATCCAGG GAACTGTAGT GAGGTCGACC ACCATACCCG AGGAATTGGG ACGAATCTCT 
TATGTATTGA CCGACAAGAC GGGCACACTC ACACAAAACG AGATGGTGTT CAAGAAGATC 
CACTTGGGCA TTGTATCGCA CGATGCAGAC ACTTTCCATC ACATTGGGCA GATGATACAG 
AAGCTATCGG GAAACATTCT GCAACAGCAG CAGGGTAGTT TATCGAGCTC GAGCAGCGGC 
GGAGACTCGA CCAAGCCAAT GATGTTTGGC AACAGGATGC GTCGACCGGA GGGATGGCGG 
GAATGGGAGG CGGTGCGAGC ACTGGCCCTG TGCCACAATG TTACACCGGT AAGCGATGAC 
GAGGACAACC GATCCGTGTC CACGGCATCG ACCGTTACCG GCGGTAACAA CTCGCCAACC 
AAATCTGTGA TAAACATCGA GGCACCTGGC AGTACGGACA CAGAGCATCA GTATCAGGCT 
GCGTCTCCAG ACGAGATTGC ACTAGTAAAG TGGACTGAGC AGGTGGGACT GACCCTGATT 
GCCAGGGATC TCAACACGAT GACGTTGCAG GTGAAGACCC CCAGCGAAGA CAATGACATA 
CTTCTGCACT ATCAAATCTT GCAGCTTTTC CCATTCACTA GCGAGAGCAA GCGCATGGGA 
ATTATAGTGC GTGAGAGCAA AACTGGTCAG ATAACGTTTT ATTTGAAGGG TGCCGACGTG 
GTTATGTCCA GTATTGTGCA GTACAACGAC TGGCTAAGCG AGGAGTCAGG AAACATGGCT 
AGAGAGGGTC TTCGCACTCT TGTTGTGGCT AAGAAGGTTC TCACCGAGGA ACAGTACTCT 
GACTTTGAGA CGCGCTACAA TGCTGCTCGA CTGAGCATCA CCGATCGTGT CGCCAAAGTG 
GCGGCAGTCA CTGAATCCCT CGAGCGTGAA CTGGAACTGC TCTGTTTGAC AGGAGTGGAG 
GATCGTCTGC AGGAGAACGT GCGTCCAACG CTGGAGCTAC TGCGCAATGC TGGAGTTCGC 
GTTTGGATGC TAACGGGCGA CAAGCTGGAG ACTGCTTGCT GTATTGCCAA GTCCTCACAG 
CTGATTGGTC GGAACCAGGG ACTCCATGTT TTGCGTTCCG TAAAGACGCG TACCGATGCT 
CACCAGGAGC TGAACAGCTT CCGGCGTAAG CAGGGTCATG CACTGGTCAT CTCAGGCGAA 
TCCCTCGAGG TTTGCCTGCA GTATTATAGA CCAGAGTTCC TGGAGCTGGC CACGGCCTCG 
CCGGCCGTCG TTTGCTGTCG CTGCTCGCCC ACACAGAAAG CACAGGTGGT GGCGCTCATA 
CAAAAGCACA CAGGCAAACG AACCTGTGCC GTGGGTGACG GTGGCAACGA TGTCAGCATG 
ATTCAGCAGG CGGACGCTGG AGTTGGAATT GAAGGACGAG AGGGAAGACA AGCCTCTCTG 
GCCGGCGATT TTAGCATTCC GCAGTTTTCG CACATCGCCA AGCTCCTGCT TATCCACGGT 
CGCAGGAGCT ACAAGCGATC GGCGGCACTA TCCCAGTTCG TTATCCACCG CGGCTTGATC 
ATAACCACAT TGCAGGCGGT CTTTTCAGCG GTATTCTACC TCAGCTCGGT TGCTTTGTAT 
CAGGGCTTTC TCATGGTCGG TTACTCGACG CTGTACACTA TGTTCCCGGT GTTCTCATTA 
GTGCTAGATC AGGATATCAC ATCGGAGACG GCCGTCACAT ACCCAGAGCT TTACAAGGAT 
TTGTCAAAAG GACGCAGTCT AAGCTACAAG ACCTTCTTTA TATGGGTACT AATCAGCATA 
TACCAAGGCG GCGTCATCAT GTACGGAGCA TTAATACTCT TTGTGGATGA GTTTATCCAC 
ATTGTGGCGA TCAGTTTCTC GGCATTAATC ATGACTGAGC TCATCATGGT GGCTCTCACC 
GTGCGTACTT GGCATAGGTT AATGGTGCTA GCGGAACTAT TCAGTTTAGC GCTTTATCTC 
ATCTCATTGG CCGTGTTACA TGAGTATTTC GACTGGGAGT TTATTTGGTC GTATGATTTT 
CTGTGGAAGG TTTCTCTCAT CACTCTGGTC TCCTGCTTAC CATTGTACAT AATCAAGTTC 
TTGCGAAAGA AATGTTCGCC GCCCTCTTAC TTGAAGCTCT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285887.1
CDS	564..3548
Translation	MALSQFIPDIRIGYPYTYWGPLGFVLMVTICREAVDDLRRHQRDHEVNSQKYKRLSSTNISGYEMVPSSKLKVGDVIIVEKNERVPADLILLRTSDRSGSVFVRTDQLDGETDWKPRLAVPYTQKLSRDSELHSIDASFYVEKPQNDIHSFIATFCMADGSEDTGLSVENTLWANTVVAAGTATGIVIYTGCETRSVMNNSQPRSKVGLLDMEINGLTKVLFCAVLGLSLVMMMLKGFGGPWYRYMFRFVLLFSYIIPISLRVNLDMGKAFYSWQMQNDSNIQGTVVRSTTIPEELGRISYVLTDKTGTLTQNEMVFKKIHLGIVSHDADTFHHIGQMIQKLSGNILQQQQGSLSSSSSGGDSTKPMMFGNRMRRPEGWREWEAVRALALCHNVTPVSDDEDNRSVSTASTVTGGNNSPTKSVINIEAPGSTDTEHQYQAASPDEIALVKWTEQVGLTLIARDLNTMTLQVKTPSEDNDILLHYQILQLFPFTSESKRMGIIVRESKTGQITFYLKGADVVMSSIVQYNDWLSEESGNMAREGLRTLVVAKKVLTEEQYSDFETRYNAARLSITDRVAKVAAVTESLERELELLCLTGVEDRLQENVRPTLELLRNAGVRVWMLTGDKLETACCIAKSSQLIGRNQGLHVLRSVKTRTDAHQELNSFRRKQGHALVISGESLEVCLQYYRPEFLELATASPAVVCCRCSPTQKAQVVALIQKHTGKRTCAVGDGGNDVSMIQQADAGVGIEGREGRQASLAGDFSIPQFSHIAKLLLIHGRRSYKRSAALSQFVIHRGLIITTLQAVFSAVFYLSSVALYQGFLMVGYSTLYTMFPVFSLVLDQDITSETAVTYPELYKDLSKGRSLSYKTFFIWVLISIYQGGVIMYGALILFVDEFIHIVAISFSALIMTELIMVALTVRTWHRLMVLAELFSLALYLISLAVLHEYFDWEFIWSYDFLWKVSLITLVSCLPLYIIKFLRKKCSPPSYLKLS

Target ORF information:

RefSeq Version	NM_001298958.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG31729), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298958.1

ORF Insert Sequence:

ATGGCGCTGT CGCAGTTCAT TCCGGACATC CGGATCGGTT ACCCGTACAC CTACTGGGGT 
CCGCTCGGCT TCGTGCTAAT GGTCACCATT TGCCGAGAGG CGGTCGACGA TCTGCGTCGG 
CATCAGCGGG ATCACGAGGT AAACTCGCAG AAGTACAAGA GACTGTCGTC CACCAACATC 
AGCGGCTACG AAATGGTTCC CAGCTCCAAG CTGAAGGTCG GTGACGTCAT AATCGTGGAA 
AAGAACGAGC GCGTGCCAGC GGATCTGATC CTGCTGCGCA CCAGCGATCG GAGCGGATCG 
GTCTTTGTTC GTACGGACCA ACTTGATGGC GAGACGGACT GGAAACCACG TCTCGCCGTT 
CCGTATACTC AAAAACTCAG TCGAGATTCG GAGCTACATA GCATCGATGC CAGTTTTTAC 
GTGGAGAAGC CACAGAACGA CATTCACAGC TTCATCGCCA CCTTCTGCAT GGCAGACGGC 
AGCGAAGACA CCGGTCTCAG TGTGGAGAAT ACCCTGTGGG CCAACACCGT CGTAGCCGCA 
GGAACAGCCA CAGGAATTGT CATATATACT GGCTGCGAAA CTCGCAGCGT GATGAATAAC 
TCACAGCCAA GGTCCAAAGT TGGACTGCTA GACATGGAGA TTAACGGATT GACTAAGGTT 
CTGTTCTGCG CCGTGCTTGG ACTGTCGCTA GTCATGATGA TGCTTAAGGG TTTCGGCGGT 
CCTTGGTATA GATACATGTT CCGCTTCGTT CTTCTCTTCT CCTACATCAT TCCAATCAGT 
CTGCGTGTCA ATTTGGACAT GGGAAAGGCG TTCTACTCAT GGCAAATGCA AAACGATTCG 
AACATCCAGG GAACTGTAGT GAGGTCGACC ACCATACCCG AGGAATTGGG ACGAATCTCT 
TATGTATTGA CCGACAAGAC GGGCACACTC ACACAAAACG AGATGGTGTT CAAGAAGATC 
CACTTGGGCA TTGTATCGCA CGATGCAGAC ACTTTCCATC ACATTGGGCA GATGATACAG 
AAGCTATCGG GAAACATTCT GCAACAGCAG CAGGGTAGTT TATCGAGCTC GAGCAGCGGC 
GGAGACTCGA CCAAGCCAAT GATGTTTGGC AACAGGATGC GTCGACCGGA GGGATGGCGG 
GAATGGGAGG CGGTGCGAGC ACTGGCCCTG TGCCACAATG TTACACCGGT AAGCGATGAC 
GAGGACAACC GATCCGTGTC CACGGCATCG ACCGTTACCG GCGGTAACAA CTCGCCAACC 
AAATCTGTGA TAAACATCGA GGCACCTGGC AGTACGGACA CAGAGCATCA GTATCAGGCT 
GCGTCTCCAG ACGAGATTGC ACTAGTAAAG TGGACTGAGC AGGTGGGACT GACCCTGATT 
GCCAGGGATC TCAACACGAT GACGTTGCAG GTGAAGACCC CCAGCGAAGA CAATGACATA 
CTTCTGCACT ATCAAATCTT GCAGCTTTTC CCATTCACTA GCGAGAGCAA GCGCATGGGA 
ATTATAGTGC GTGAGAGCAA AACTGGTCAG ATAACGTTTT ATTTGAAGGG TGCCGACGTG 
GTTATGTCCA GTATTGTGCA GTACAACGAC TGGCTAAGCG AGGAGTCAGG AAACATGGCT 
AGAGAGGGTC TTCGCACTCT TGTTGTGGCT AAGAAGGTTC TCACCGAGGA ACAGTACTCT 
GACTTTGAGA CGCGCTACAA TGCTGCTCGA CTGAGCATCA CCGATCGTGT CGCCAAAGTG 
GCGGCAGTCA CTGAATCCCT CGAGCGTGAA CTGGAACTGC TCTGTTTGAC AGGAGTGGAG 
GATCGTCTGC AGGAGAACGT GCGTCCAACG CTGGAGCTAC TGCGCAATGC TGGAGTTCGC 
GTTTGGATGC TAACGGGCGA CAAGCTGGAG ACTGCTTGCT GTATTGCCAA GTCCTCACAG 
CTGATTGGTC GGAACCAGGG ACTCCATGTT TTGCGTTCCG TAAAGACGCG TACCGATGCT 
CACCAGGAGC TGAACAGCTT CCGGCGTAAG CAGGGTCATG CACTGGTCAT CTCAGGCGAA 
TCCCTCGAGG TTTGCCTGCA GTATTATAGA CCAGAGTTCC TGGAGCTGGC CACGGCCTCG 
CCGGCCGTCG TTTGCTGTCG CTGCTCGCCC ACACAGAAAG CACAGGTGGT GGCGCTCATA 
CAAAAGCACA CAGGCAAACG AACCTGTGCC GTGGGTGACG GTGGCAACGA TGTCAGCATG 
ATTCAGCAGG CGGACGCTGG AGTTGGAATT GAAGGACGAG AGGGAAGACA AGCCTCTCTG 
GCCGGCGATT TTAGCATTCC GCAGTTTTCG CACATCGCCA AGCTCCTGCT TATCCACGGT 
CGCAGGAGCT ACAAGCGATC GGCGGCACTA TCCCAGTTCG TTATCCACCG CGGCTTGATC 
ATAACCACAT TGCAGGCGGT CTTTTCAGCG GTATTCTACC TCAGCTCGGT TGCTTTGTAT 
CAGGGCTTTC TCATGGTCGG TTACTCGACG CTGTACACTA TGTTCCCGGT GTTCTCATTA 
GTGCTAGATC AGGATATCAC ATCGGAGACG GCCGTCACAT ACCCAGAGCT TTACAAGGAT 
TTGTCAAAAG GACGCAGTCT AAGCTACAAG ACCTTCTTTA TATGGGTACT AATCAGCATA 
TACCAAGGCG GCGTCATCAT GTACGGAGCA TTAATACTCT TTGTGGATGA GTTTATCCAC 
ATTGTGGCGA TCAGTTTCTC GGCATTAATC ATGACTGAGC TCATCATGGT GGCTCTCACC 
GTGCGTACTT GGCATAGGTT AATGGTGCTA GCGGAACTAT TCAGTTTAGC GCTTTATCTC 
ATCTCATTGG CCGTGTTACA TGAGTATTTC GACTGGGAGT TTATTTGGTC GTATGATTTT 
CTGTGGAAGG TTTCTCTCAT CACTCTGGTC TCCTGCTTAC CATTGTACAT AATCAAGTTC 
TTGCGAAAGA AATGTTCGCC GCCCTCTTAC TTGAAGCTCT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG31729 ( NM_165038.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723806.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG31729), mRNA.

Price & Availability↑

CloneID	OFa14637
Clone ID Related Accession (Same CDS sequence)	NM_165038.3
Accession Version	NM_165038.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165038.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGATG CTACCTCCCA AAACTCGGCT GCAACGCCCA TGACACGGGA GAACATTCCG 
TTGCTGAATC GTCAGCGAAA TCGCTGGTAT CTCTTTAGCT GCTGGCGAAA ATGGTTCCGG 
CCACGGGAGC TGAGAGCCCG CACCGTAAAT CTCGGACGGG TCAATACGGA GAAGTTCCCA 
CCGAACGAGA TCCGGAATCA GAAGTACAAC TTCATCACCT TCCTGCCGCT GGTGCTGTTC 
GAGCAGTTTC GCTTCTTCCT CAACTTGTAC TTCCTGCTGA TGGCGCTGTC GCAGTTCATT 
CCGGACATCC GGATCGGTTA CCCGTACACC TACTGGGGTC CGCTCGGCTT CGTGCTAATG 
GTCACCATTT GCCGAGAGGC GGTCGACGAT CTGCGTCGGC ATCAGCGGGA TCACGAGGTA 
AACTCGCAGA AGTACAAGAG ACTGTCGTCC ACCAACATCA GCGGCTACGA AATGGTTCCC 
AGCTCCAAGC TGAAGGTCGG TGACGTCATA ATCGTGGAAA AGAACGAGCG CGTGCCAGCG 
GATCTGATCC TGCTGCGCAC CAGCGATCGG AGCGGATCGG TCTTTGTTCG TACGGACCAA 
CTTGATGGCG AGACGGACTG GAAACCACGT CTCGCCGTTC CGTATACTCA AAAACTCAGT 
CGAGATTCGG AGCTACATAG CATCGATGCC AGTTTTTACG TGGAGAAGCC ACAGAACGAC 
ATTCACAGCT TCATCGCCAC CTTCTGCATG GCAGACGGCA GCGAAGACAC CGGTCTCAGT 
GTGGAGAATA CCCTGTGGGC CAACACCGTC GTAGCCGCAG GAACAGCCAC AGGAATTGTC 
ATATATACTG GCTGCGAAAC TCGCAGCGTG ATGAATAACT CACAGCCAAG GTCCAAAGTT 
GGACTGCTAG ACATGGAGAT TAACGGATTG ACTAAGGTTC TGTTCTGCGC CGTGCTTGGA 
CTGTCGCTAG TCATGATGAT GCTTAAGGGT TTCGGCGGTC CTTGGTATAG ATACATGTTC 
CGCTTCGTTC TTCTCTTCTC CTACATCATT CCAATCAGTC TGCGTGTCAA TTTGGACATG 
GGAAAGGCGT TCTACTCATG GCAAATGCAA AACGATTCGA ACATCCAGGG AACTGTAGTG 
AGGTCGACCA CCATACCCGA GGAATTGGGA CGAATCTCTT ATGTATTGAC CGACAAGACG 
GGCACACTCA CACAAAACGA GATGGTGTTC AAGAAGATCC ACTTGGGCAT TGTATCGCAC 
GATGCAGACA CTTTCCATCA CATTGGGCAG ATGATACAGA AGCTATCGGG AAACATTCTG 
CAACAGCAGC AGGGTAGTTT ATCGAGCTCG AGCAGCGGCG GAGACTCGAC CAAGCCAATG 
ATGTTTGGCA ACAGGATGCG TCGACCGGAG GGATGGCGGG AATGGGAGGC GGTGCGAGCA 
CTGGCCCTGT GCCACAATGT TACACCGGTA AGCGATGACG AGGACAACCG ATCCGTGTCC 
ACGGCATCGA CCGTTACCGG CGGTAACAAC TCGCCAACCA AATCTGTGAT AAACATCGAG 
GCACCTGGCA GTACGGACAC AGAGCATCAG TATCAGGCTG CGTCTCCAGA CGAGATTGCA 
CTAGTAAAGT GGACTGAGCA GGTGGGACTG ACCCTGATTG CCAGGGATCT CAACACGATG 
ACGTTGCAGG TGAAGACCCC CAGCGAAGAC AATGACATAC TTCTGCACTA TCAAATCTTG 
CAGCTTTTCC CATTCACTAG CGAGAGCAAG CGCATGGGAA TTATAGTGCG TGAGAGCAAA 
ACTGGTCAGA TAACGTTTTA TTTGAAGGGT GCCGACGTGG TTATGTCCAG TATTGTGCAG 
TACAACGACT GGCTAAGCGA GGAGTCAGGA AACATGGCTA GAGAGGGTCT TCGCACTCTT 
GTTGTGGCTA AGAAGGTTCT CACCGAGGAA CAGTACTCTG ACTTTGAGAC GCGCTACAAT 
GCTGCTCGAC TGAGCATCAC CGATCGTGTC GCCAAAGTGG CGGCAGTCAC TGAATCCCTC 
GAGCGTGAAC TGGAACTGCT CTGTTTGACA GGAGTGGAGG ATCGTCTGCA GGAGAACGTG 
CGTCCAACGC TGGAGCTACT GCGCAATGCT GGAGTTCGCG TTTGGATGCT AACGGGCGAC 
AAGCTGGAGA CTGCTTGCTG TATTGCCAAG TCCTCACAGC TGATTGGTCG GAACCAGGGA 
CTCCATGTTT TGCGTTCCGT AAAGACGCGT ACCGATGCTC ACCAGGAGCT GAACAGCTTC 
CGGCGTAAGC AGGGTCATGC ACTGGTCATC TCAGGCGAAT CCCTCGAGGT TTGCCTGCAG 
TATTATAGAC CAGAGTTCCT GGAGCTGGCC ACGGCCTCGC CGGCCGTCGT TTGCTGTCGC 
TGCTCGCCCA CACAGAAAGC ACAGGTGGTG GCGCTCATAC AAAAGCACAC AGGCAAACGA 
ACCTGTGCCG TGGGTGACGG TGGCAACGAT GTCAGCATGA TTCAGCAGGC GGACGCTGGA 
GTTGGAATTG AAGGACGAGA GGGAAGACAA GCCTCTCTGG CCGGCGATTT TAGCATTCCG 
CAGTTTTCGC ACATCGCCAA GCTCCTGCTT ATCCACGGTC GCAGGAGCTA CAAGCGATCG 
GCGGCACTAT CCCAGTTCGT TATCCACCGC GGCTTGATCA TAACCACATT GCAGGCGGTC 
TTTTCAGCGG TATTCTACCT CAGCTCGGTT GCTTTGTATC AGGGCTTTCT CATGGTCGGT 
TACTCGACGC TGTACACTAT GTTCCCGGTG TTCTCATTAG TGCTAGATCA GGATATCACA 
TCGGAGACGG CCGTCACATA CCCAGAGCTT TACAAGGATT TGTCAAAAGG ACGCAGTCTA 
AGCTACAAGA CCTTCTTTAT ATGGGTACTA ATCAGCATAT ACCAAGGCGG CGTCATCATG 
TACGGAGCAT TAATACTCTT TGTGGATGAG TTTATCCACA TTGTGGCGAT CAGTTTCTCG 
GCATTAATCA TGACTGAGCT CATCATGGTG GCTCTCACCG TGCGTACTTG GCATAGGTTA 
ATGGTGCTAG CGGAACTATT CAGTTTAGCG CTTTATCTCA TCTCATTGGC CGTGTTACAT 
GAGTATTTCG ACTGGGAGTT TATTTGGTCG TATGATTTTC TGTGGAAGGT TTCTCTCATC 
ACTCTGGTCT CCTGCTTACC ATTGTACATA ATCAAGTTCT TGCGAAAGAA ATGTTCGCCG 
CCCTCTTACT TGAAGCTCTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723806.1
CDS	301..3564
Translation	MDDATSQNSAATPMTRENIPLLNRQRNRWYLFSCWRKWFRPRELRARTVNLGRVNTEKFPPNEIRNQKYNFITFLPLVLFEQFRFFLNLYFLLMALSQFIPDIRIGYPYTYWGPLGFVLMVTICREAVDDLRRHQRDHEVNSQKYKRLSSTNISGYEMVPSSKLKVGDVIIVEKNERVPADLILLRTSDRSGSVFVRTDQLDGETDWKPRLAVPYTQKLSRDSELHSIDASFYVEKPQNDIHSFIATFCMADGSEDTGLSVENTLWANTVVAAGTATGIVIYTGCETRSVMNNSQPRSKVGLLDMEINGLTKVLFCAVLGLSLVMMMLKGFGGPWYRYMFRFVLLFSYIIPISLRVNLDMGKAFYSWQMQNDSNIQGTVVRSTTIPEELGRISYVLTDKTGTLTQNEMVFKKIHLGIVSHDADTFHHIGQMIQKLSGNILQQQQGSLSSSSSGGDSTKPMMFGNRMRRPEGWREWEAVRALALCHNVTPVSDDEDNRSVSTASTVTGGNNSPTKSVINIEAPGSTDTEHQYQAASPDEIALVKWTEQVGLTLIARDLNTMTLQVKTPSEDNDILLHYQILQLFPFTSESKRMGIIVRESKTGQITFYLKGADVVMSSIVQYNDWLSEESGNMAREGLRTLVVAKKVLTEEQYSDFETRYNAARLSITDRVAKVAAVTESLERELELLCLTGVEDRLQENVRPTLELLRNAGVRVWMLTGDKLETACCIAKSSQLIGRNQGLHVLRSVKTRTDAHQELNSFRRKQGHALVISGESLEVCLQYYRPEFLELATASPAVVCCRCSPTQKAQVVALIQKHTGKRTCAVGDGGNDVSMIQQADAGVGIEGREGRQASLAGDFSIPQFSHIAKLLLIHGRRSYKRSAALSQFVIHRGLIITTLQAVFSAVFYLSSVALYQGFLMVGYSTLYTMFPVFSLVLDQDITSETAVTYPELYKDLSKGRSLSYKTFFIWVLISIYQGGVIMYGALILFVDEFIHIVAISFSALIMTELIMVALTVRTWHRLMVLAELFSLALYLISLAVLHEYFDWEFIWSYDFLWKVSLITLVSCLPLYIIKFLRKKCSPPSYLKLS

Target ORF information:

RefSeq Version	NM_165038.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31729), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165038.3

ORF Insert Sequence:

ATGGATGATG CTACCTCCCA AAACTCGGCT GCAACGCCCA TGACACGGGA GAACATTCCG 
TTGCTGAATC GTCAGCGAAA TCGCTGGTAT CTCTTTAGCT GCTGGCGAAA ATGGTTCCGG 
CCACGGGAGC TGAGAGCCCG CACCGTAAAT CTCGGACGGG TCAATACGGA GAAGTTCCCA 
CCGAACGAGA TCCGGAATCA GAAGTACAAC TTCATCACCT TCCTGCCGCT GGTGCTGTTC 
GAGCAGTTTC GCTTCTTCCT CAACTTGTAC TTCCTGCTGA TGGCGCTGTC GCAGTTCATT 
CCGGACATCC GGATCGGTTA CCCGTACACC TACTGGGGTC CGCTCGGCTT CGTGCTAATG 
GTCACCATTT GCCGAGAGGC GGTCGACGAT CTGCGTCGGC ATCAGCGGGA TCACGAGGTA 
AACTCGCAGA AGTACAAGAG ACTGTCGTCC ACCAACATCA GCGGCTACGA AATGGTTCCC 
AGCTCCAAGC TGAAGGTCGG TGACGTCATA ATCGTGGAAA AGAACGAGCG CGTGCCAGCG 
GATCTGATCC TGCTGCGCAC CAGCGATCGG AGCGGATCGG TCTTTGTTCG TACGGACCAA 
CTTGATGGCG AGACGGACTG GAAACCACGT CTCGCCGTTC CGTATACTCA AAAACTCAGT 
CGAGATTCGG AGCTACATAG CATCGATGCC AGTTTTTACG TGGAGAAGCC ACAGAACGAC 
ATTCACAGCT TCATCGCCAC CTTCTGCATG GCAGACGGCA GCGAAGACAC CGGTCTCAGT 
GTGGAGAATA CCCTGTGGGC CAACACCGTC GTAGCCGCAG GAACAGCCAC AGGAATTGTC 
ATATATACTG GCTGCGAAAC TCGCAGCGTG ATGAATAACT CACAGCCAAG GTCCAAAGTT 
GGACTGCTAG ACATGGAGAT TAACGGATTG ACTAAGGTTC TGTTCTGCGC CGTGCTTGGA 
CTGTCGCTAG TCATGATGAT GCTTAAGGGT TTCGGCGGTC CTTGGTATAG ATACATGTTC 
CGCTTCGTTC TTCTCTTCTC CTACATCATT CCAATCAGTC TGCGTGTCAA TTTGGACATG 
GGAAAGGCGT TCTACTCATG GCAAATGCAA AACGATTCGA ACATCCAGGG AACTGTAGTG 
AGGTCGACCA CCATACCCGA GGAATTGGGA CGAATCTCTT ATGTATTGAC CGACAAGACG 
GGCACACTCA CACAAAACGA GATGGTGTTC AAGAAGATCC ACTTGGGCAT TGTATCGCAC 
GATGCAGACA CTTTCCATCA CATTGGGCAG ATGATACAGA AGCTATCGGG AAACATTCTG 
CAACAGCAGC AGGGTAGTTT ATCGAGCTCG AGCAGCGGCG GAGACTCGAC CAAGCCAATG 
ATGTTTGGCA ACAGGATGCG TCGACCGGAG GGATGGCGGG AATGGGAGGC GGTGCGAGCA 
CTGGCCCTGT GCCACAATGT TACACCGGTA AGCGATGACG AGGACAACCG ATCCGTGTCC 
ACGGCATCGA CCGTTACCGG CGGTAACAAC TCGCCAACCA AATCTGTGAT AAACATCGAG 
GCACCTGGCA GTACGGACAC AGAGCATCAG TATCAGGCTG CGTCTCCAGA CGAGATTGCA 
CTAGTAAAGT GGACTGAGCA GGTGGGACTG ACCCTGATTG CCAGGGATCT CAACACGATG 
ACGTTGCAGG TGAAGACCCC CAGCGAAGAC AATGACATAC TTCTGCACTA TCAAATCTTG 
CAGCTTTTCC CATTCACTAG CGAGAGCAAG CGCATGGGAA TTATAGTGCG TGAGAGCAAA 
ACTGGTCAGA TAACGTTTTA TTTGAAGGGT GCCGACGTGG TTATGTCCAG TATTGTGCAG 
TACAACGACT GGCTAAGCGA GGAGTCAGGA AACATGGCTA GAGAGGGTCT TCGCACTCTT 
GTTGTGGCTA AGAAGGTTCT CACCGAGGAA CAGTACTCTG ACTTTGAGAC GCGCTACAAT 
GCTGCTCGAC TGAGCATCAC CGATCGTGTC GCCAAAGTGG CGGCAGTCAC TGAATCCCTC 
GAGCGTGAAC TGGAACTGCT CTGTTTGACA GGAGTGGAGG ATCGTCTGCA GGAGAACGTG 
CGTCCAACGC TGGAGCTACT GCGCAATGCT GGAGTTCGCG TTTGGATGCT AACGGGCGAC 
AAGCTGGAGA CTGCTTGCTG TATTGCCAAG TCCTCACAGC TGATTGGTCG GAACCAGGGA 
CTCCATGTTT TGCGTTCCGT AAAGACGCGT ACCGATGCTC ACCAGGAGCT GAACAGCTTC 
CGGCGTAAGC AGGGTCATGC ACTGGTCATC TCAGGCGAAT CCCTCGAGGT TTGCCTGCAG 
TATTATAGAC CAGAGTTCCT GGAGCTGGCC ACGGCCTCGC CGGCCGTCGT TTGCTGTCGC 
TGCTCGCCCA CACAGAAAGC ACAGGTGGTG GCGCTCATAC AAAAGCACAC AGGCAAACGA 
ACCTGTGCCG TGGGTGACGG TGGCAACGAT GTCAGCATGA TTCAGCAGGC GGACGCTGGA 
GTTGGAATTG AAGGACGAGA GGGAAGACAA GCCTCTCTGG CCGGCGATTT TAGCATTCCG 
CAGTTTTCGC ACATCGCCAA GCTCCTGCTT ATCCACGGTC GCAGGAGCTA CAAGCGATCG 
GCGGCACTAT CCCAGTTCGT TATCCACCGC GGCTTGATCA TAACCACATT GCAGGCGGTC 
TTTTCAGCGG TATTCTACCT CAGCTCGGTT GCTTTGTATC AGGGCTTTCT CATGGTCGGT 
TACTCGACGC TGTACACTAT GTTCCCGGTG TTCTCATTAG TGCTAGATCA GGATATCACA 
TCGGAGACGG CCGTCACATA CCCAGAGCTT TACAAGGATT TGTCAAAAGG ACGCAGTCTA 
AGCTACAAGA CCTTCTTTAT ATGGGTACTA ATCAGCATAT ACCAAGGCGG CGTCATCATG 
TACGGAGCAT TAATACTCTT TGTGGATGAG TTTATCCACA TTGTGGCGAT CAGTTTCTCG 
GCATTAATCA TGACTGAGCT CATCATGGTG GCTCTCACCG TGCGTACTTG GCATAGGTTA 
ATGGTGCTAG CGGAACTATT CAGTTTAGCG CTTTATCTCA TCTCATTGGC CGTGTTACAT 
GAGTATTTCG ACTGGGAGTT TATTTGGTCG TATGATTTTC TGTGGAAGGT TTCTCTCATC 
ACTCTGGTCT CCTGCTTACC ATTGTACATA ATCAAGTTCT TGCGAAAGAA ATGTTCGCCG 
CCCTCTTACT TGAAGCTCTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG31729 ( NM_001298957.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285886.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG31729), mRNA.

Price & Availability↑

CloneID	OFa21641
Clone ID Related Accession (Same CDS sequence)	NM_001298957.1
Accession Version	NM_001298957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAGC CCCAAGGCTT CGGCGGTAAG AGCGCTGACT TTGAAGTGCA GCATGAGTCA 
TACAGCTGCT GGCGAAAATG GTTCCGGCCA CGGGAGCTGA GAGCCCGCAC CGTAAATCTC 
GGACGGGTCA ATACGGAGAA GTTCCCACCG AACGAGATCC GGAATCAGAA GTACAACTTC 
ATCACCTTCC TGCCGCTGGT GCTGTTCGAG CAGTTTCGCT TCTTCCTCAA CTTGTACTTC 
CTGCTGATGG CGCTGTCGCA GTTCATTCCG GACATCCGGA TCGGTTACCC GTACACCTAC 
TGGGGTCCGC TCGGCTTCGT GCTAATGGTC ACCATTTGCC GAGAGGCGGT CGACGATCTG 
CGTCGGCATC AGCGGGATCA CGAGGTAAAC TCGCAGAAGT ACAAGAGACT GTCGTCCACC 
AACATCAGCG GCTACGAAAT GGTTCCCAGC TCCAAGCTGA AGGTCGGTGA CGTCATAATC 
GTGGAAAAGA ACGAGCGCGT GCCAGCGGAT CTGATCCTGC TGCGCACCAG CGATCGGAGC 
GGATCGGTCT TTGTTCGTAC GGACCAACTT GATGGCGAGA CGGACTGGAA ACCACGTCTC 
GCCGTTCCGT ATACTCAAAA ACTCAGTCGA GATTCGGAGC TACATAGCAT CGATGCCAGT 
TTTTACGTGG AGAAGCCACA GAACGACATT CACAGCTTCA TCGCCACCTT CTGCATGGCA 
GACGGCAGCG AAGACACCGG TCTCAGTGTG GAGAATACCC TGTGGGCCAA CACCGTCGTA 
GCCGCAGGAA CAGCCACAGG AATTGTCATA TATACTGGCT GCGAAACTCG CAGCGTGATG 
AATAACTCAC AGCCAAGGTC CAAAGTTGGA CTGCTAGACA TGGAGATTAA CGGATTGACT 
AAGGTTCTGT TCTGCGCCGT GCTTGGACTG TCGCTAGTCA TGATGATGCT TAAGGGTTTC 
GGCGGTCCTT GGTATAGATA CATGTTCCGC TTCGTTCTTC TCTTCTCCTA CATCATTCCA 
ATCAGTCTGC GTGTCAATTT GGACATGGGA AAGGCGTTCT ACTCATGGCA AATGCAAAAC 
GATTCGAACA TCCAGGGAAC TGTAGTGAGG TCGACCACCA TACCCGAGGA ATTGGGACGA 
ATCTCTTATG TATTGACCGA CAAGACGGGC ACACTCACAC AAAACGAGAT GGTGTTCAAG 
AAGATCCACT TGGGCATTGT ATCGCACGAT GCAGACACTT TCCATCACAT TGGGCAGATG 
ATACAGAAGC TATCGGGAAA CATTCTGCAA CAGCAGCAGG GTAGTTTATC GAGCTCGAGC 
AGCGGCGGAG ACTCGACCAA GCCAATGATG TTTGGCAACA GGATGCGTCG ACCGGAGGGA 
TGGCGGGAAT GGGAGGCGGT GCGAGCACTG GCCCTGTGCC ACAATGTTAC ACCGGTAAGC 
GATGACGAGG ACAACCGATC CGTGTCCACG GCATCGACCG TTACCGGCGG TAACAACTCG 
CCAACCAAAT CTGTGATAAA CATCGAGGCA CCTGGCAGTA CGGACACAGA GCATCAGTAT 
CAGGCTGCGT CTCCAGACGA GATTGCACTA GTAAAGTGGA CTGAGCAGGT GGGACTGACC 
CTGATTGCCA GGGATCTCAA CACGATGACG TTGCAGGTGA AGACCCCCAG CGAAGACAAT 
GACATACTTC TGCACTATCA AATCTTGCAG CTTTTCCCAT TCACTAGCGA GAGCAAGCGC 
ATGGGAATTA TAGTGCGTGA GAGCAAAACT GGTCAGATAA CGTTTTATTT GAAGGGTGCC 
GACGTGGTTA TGTCCAGTAT TGTGCAGTAC AACGACTGGC TAAGCGAGGA GTCAGGAAAC 
ATGGCTAGAG AGGGTCTTCG CACTCTTGTT GTGGCTAAGA AGGTTCTCAC CGAGGAACAG 
TACTCTGACT TTGAGACGCG CTACAATGCT GCTCGACTGA GCATCACCGA TCGTGTCGCC 
AAAGTGGCGG CAGTCACTGA ATCCCTCGAG CGTGAACTGG AACTGCTCTG TTTGACAGGA 
GTGGAGGATC GTCTGCAGGA GAACGTGCGT CCAACGCTGG AGCTACTGCG CAATGCTGGA 
GTTCGCGTTT GGATGCTAAC GGGCGACAAG CTGGAGACTG CTTGCTGTAT TGCCAAGTCC 
TCACAGCTGA TTGGTCGGAA CCAGGGACTC CATGTTTTGC GTTCCGTAAA GACGCGTACC 
GATGCTCACC AGGAGCTGAA CAGCTTCCGG CGTAAGCAGG GTCATGCACT GGTCATCTCA 
GGCGAATCCC TCGAGGTTTG CCTGCAGTAT TATAGACCAG AGTTCCTGGA GCTGGCCACG 
GCCTCGCCGG CCGTCGTTTG CTGTCGCTGC TCGCCCACAC AGAAAGCACA GGTGGTGGCG 
CTCATACAAA AGCACACAGG CAAACGAACC TGTGCCGTGG GTGACGGTGG CAACGATGTC 
AGCATGATTC AGCAGGCGGA CGCTGGAGTT GGAATTGAAG GACGAGAGGG AAGACAAGCC 
TCTCTGGCCG GCGATTTTAG CATTCCGCAG TTTTCGCACA TCGCCAAGCT CCTGCTTATC 
CACGGTCGCA GGAGCTACAA GCGATCGGCG GCACTATCCC AGTTCGTTAT CCACCGCGGC 
TTGATCATAA CCACATTGCA GGCGGTCTTT TCAGCGGTAT TCTACCTCAG CTCGGTTGCT 
TTGTATCAGG GCTTTCTCAT GGTCGGTTAC TCGACGCTGT ACACTATGTT CCCGGTGTTC 
TCATTAGTGC TAGATCAGGA TATCACATCG GAGACGGCCG TCACATACCC AGAGCTTTAC 
AAGGATTTGT CAAAAGGACG CAGTCTAAGC TACAAGACCT TCTTTATATG GGTACTAATC 
AGCATATACC AAGGCGGCGT CATCATGTAC GGAGCATTAA TACTCTTTGT GGATGAGTTT 
ATCCACATTG TGGCGATCAG TTTCTCGGCA TTAATCATGA CTGAGCTCAT CATGGTGGCT 
CTCACCGTGC GTACTTGGCA TAGGTTAATG GTGCTAGCGG AACTATTCAG TTTAGCGCTT 
TATCTCATCT CATTGGCCGT GTTACATGAG TATTTCGACT GGGAGTTTAT TTGGTCGTAT 
GATTTTCTGT GGAAGGTTTC TCTCATCACT CTGGTCTCCT GCTTACCATT GTACATAATC 
AAGTTCTTGC GAAAGAAATG TTCGCCGCCC TCTTACTTGA AGCTCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285886.1
CDS	130..3360
Translation	MAEPQGFGGKSADFEVQHESYSCWRKWFRPRELRARTVNLGRVNTEKFPPNEIRNQKYNFITFLPLVLFEQFRFFLNLYFLLMALSQFIPDIRIGYPYTYWGPLGFVLMVTICREAVDDLRRHQRDHEVNSQKYKRLSSTNISGYEMVPSSKLKVGDVIIVEKNERVPADLILLRTSDRSGSVFVRTDQLDGETDWKPRLAVPYTQKLSRDSELHSIDASFYVEKPQNDIHSFIATFCMADGSEDTGLSVENTLWANTVVAAGTATGIVIYTGCETRSVMNNSQPRSKVGLLDMEINGLTKVLFCAVLGLSLVMMMLKGFGGPWYRYMFRFVLLFSYIIPISLRVNLDMGKAFYSWQMQNDSNIQGTVVRSTTIPEELGRISYVLTDKTGTLTQNEMVFKKIHLGIVSHDADTFHHIGQMIQKLSGNILQQQQGSLSSSSSGGDSTKPMMFGNRMRRPEGWREWEAVRALALCHNVTPVSDDEDNRSVSTASTVTGGNNSPTKSVINIEAPGSTDTEHQYQAASPDEIALVKWTEQVGLTLIARDLNTMTLQVKTPSEDNDILLHYQILQLFPFTSESKRMGIIVRESKTGQITFYLKGADVVMSSIVQYNDWLSEESGNMAREGLRTLVVAKKVLTEEQYSDFETRYNAARLSITDRVAKVAAVTESLERELELLCLTGVEDRLQENVRPTLELLRNAGVRVWMLTGDKLETACCIAKSSQLIGRNQGLHVLRSVKTRTDAHQELNSFRRKQGHALVISGESLEVCLQYYRPEFLELATASPAVVCCRCSPTQKAQVVALIQKHTGKRTCAVGDGGNDVSMIQQADAGVGIEGREGRQASLAGDFSIPQFSHIAKLLLIHGRRSYKRSAALSQFVIHRGLIITTLQAVFSAVFYLSSVALYQGFLMVGYSTLYTMFPVFSLVLDQDITSETAVTYPELYKDLSKGRSLSYKTFFIWVLISIYQGGVIMYGALILFVDEFIHIVAISFSALIMTELIMVALTVRTWHRLMVLAELFSLALYLISLAVLHEYFDWEFIWSYDFLWKVSLITLVSCLPLYIIKFLRKKCSPPSYLKLS

Target ORF information:

RefSeq Version	NM_001298957.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG31729), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298957.1

ORF Insert Sequence:

ATGGCGGAGC CCCAAGGCTT CGGCGGTAAG AGCGCTGACT TTGAAGTGCA GCATGAGTCA 
TACAGCTGCT GGCGAAAATG GTTCCGGCCA CGGGAGCTGA GAGCCCGCAC CGTAAATCTC 
GGACGGGTCA ATACGGAGAA GTTCCCACCG AACGAGATCC GGAATCAGAA GTACAACTTC 
ATCACCTTCC TGCCGCTGGT GCTGTTCGAG CAGTTTCGCT TCTTCCTCAA CTTGTACTTC 
CTGCTGATGG CGCTGTCGCA GTTCATTCCG GACATCCGGA TCGGTTACCC GTACACCTAC 
TGGGGTCCGC TCGGCTTCGT GCTAATGGTC ACCATTTGCC GAGAGGCGGT CGACGATCTG 
CGTCGGCATC AGCGGGATCA CGAGGTAAAC TCGCAGAAGT ACAAGAGACT GTCGTCCACC 
AACATCAGCG GCTACGAAAT GGTTCCCAGC TCCAAGCTGA AGGTCGGTGA CGTCATAATC 
GTGGAAAAGA ACGAGCGCGT GCCAGCGGAT CTGATCCTGC TGCGCACCAG CGATCGGAGC 
GGATCGGTCT TTGTTCGTAC GGACCAACTT GATGGCGAGA CGGACTGGAA ACCACGTCTC 
GCCGTTCCGT ATACTCAAAA ACTCAGTCGA GATTCGGAGC TACATAGCAT CGATGCCAGT 
TTTTACGTGG AGAAGCCACA GAACGACATT CACAGCTTCA TCGCCACCTT CTGCATGGCA 
GACGGCAGCG AAGACACCGG TCTCAGTGTG GAGAATACCC TGTGGGCCAA CACCGTCGTA 
GCCGCAGGAA CAGCCACAGG AATTGTCATA TATACTGGCT GCGAAACTCG CAGCGTGATG 
AATAACTCAC AGCCAAGGTC CAAAGTTGGA CTGCTAGACA TGGAGATTAA CGGATTGACT 
AAGGTTCTGT TCTGCGCCGT GCTTGGACTG TCGCTAGTCA TGATGATGCT TAAGGGTTTC 
GGCGGTCCTT GGTATAGATA CATGTTCCGC TTCGTTCTTC TCTTCTCCTA CATCATTCCA 
ATCAGTCTGC GTGTCAATTT GGACATGGGA AAGGCGTTCT ACTCATGGCA AATGCAAAAC 
GATTCGAACA TCCAGGGAAC TGTAGTGAGG TCGACCACCA TACCCGAGGA ATTGGGACGA 
ATCTCTTATG TATTGACCGA CAAGACGGGC ACACTCACAC AAAACGAGAT GGTGTTCAAG 
AAGATCCACT TGGGCATTGT ATCGCACGAT GCAGACACTT TCCATCACAT TGGGCAGATG 
ATACAGAAGC TATCGGGAAA CATTCTGCAA CAGCAGCAGG GTAGTTTATC GAGCTCGAGC 
AGCGGCGGAG ACTCGACCAA GCCAATGATG TTTGGCAACA GGATGCGTCG ACCGGAGGGA 
TGGCGGGAAT GGGAGGCGGT GCGAGCACTG GCCCTGTGCC ACAATGTTAC ACCGGTAAGC 
GATGACGAGG ACAACCGATC CGTGTCCACG GCATCGACCG TTACCGGCGG TAACAACTCG 
CCAACCAAAT CTGTGATAAA CATCGAGGCA CCTGGCAGTA CGGACACAGA GCATCAGTAT 
CAGGCTGCGT CTCCAGACGA GATTGCACTA GTAAAGTGGA CTGAGCAGGT GGGACTGACC 
CTGATTGCCA GGGATCTCAA CACGATGACG TTGCAGGTGA AGACCCCCAG CGAAGACAAT 
GACATACTTC TGCACTATCA AATCTTGCAG CTTTTCCCAT TCACTAGCGA GAGCAAGCGC 
ATGGGAATTA TAGTGCGTGA GAGCAAAACT GGTCAGATAA CGTTTTATTT GAAGGGTGCC 
GACGTGGTTA TGTCCAGTAT TGTGCAGTAC AACGACTGGC TAAGCGAGGA GTCAGGAAAC 
ATGGCTAGAG AGGGTCTTCG CACTCTTGTT GTGGCTAAGA AGGTTCTCAC CGAGGAACAG 
TACTCTGACT TTGAGACGCG CTACAATGCT GCTCGACTGA GCATCACCGA TCGTGTCGCC 
AAAGTGGCGG CAGTCACTGA ATCCCTCGAG CGTGAACTGG AACTGCTCTG TTTGACAGGA 
GTGGAGGATC GTCTGCAGGA GAACGTGCGT CCAACGCTGG AGCTACTGCG CAATGCTGGA 
GTTCGCGTTT GGATGCTAAC GGGCGACAAG CTGGAGACTG CTTGCTGTAT TGCCAAGTCC 
TCACAGCTGA TTGGTCGGAA CCAGGGACTC CATGTTTTGC GTTCCGTAAA GACGCGTACC 
GATGCTCACC AGGAGCTGAA CAGCTTCCGG CGTAAGCAGG GTCATGCACT GGTCATCTCA 
GGCGAATCCC TCGAGGTTTG CCTGCAGTAT TATAGACCAG AGTTCCTGGA GCTGGCCACG 
GCCTCGCCGG CCGTCGTTTG CTGTCGCTGC TCGCCCACAC AGAAAGCACA GGTGGTGGCG 
CTCATACAAA AGCACACAGG CAAACGAACC TGTGCCGTGG GTGACGGTGG CAACGATGTC 
AGCATGATTC AGCAGGCGGA CGCTGGAGTT GGAATTGAAG GACGAGAGGG AAGACAAGCC 
TCTCTGGCCG GCGATTTTAG CATTCCGCAG TTTTCGCACA TCGCCAAGCT CCTGCTTATC 
CACGGTCGCA GGAGCTACAA GCGATCGGCG GCACTATCCC AGTTCGTTAT CCACCGCGGC 
TTGATCATAA CCACATTGCA GGCGGTCTTT TCAGCGGTAT TCTACCTCAG CTCGGTTGCT 
TTGTATCAGG GCTTTCTCAT GGTCGGTTAC TCGACGCTGT ACACTATGTT CCCGGTGTTC 
TCATTAGTGC TAGATCAGGA TATCACATCG GAGACGGCCG TCACATACCC AGAGCTTTAC 
AAGGATTTGT CAAAAGGACG CAGTCTAAGC TACAAGACCT TCTTTATATG GGTACTAATC 
AGCATATACC AAGGCGGCGT CATCATGTAC GGAGCATTAA TACTCTTTGT GGATGAGTTT 
ATCCACATTG TGGCGATCAG TTTCTCGGCA TTAATCATGA CTGAGCTCAT CATGGTGGCT 
CTCACCGTGC GTACTTGGCA TAGGTTAATG GTGCTAGCGG AACTATTCAG TTTAGCGCTT 
TATCTCATCT CATTGGCCGT GTTACATGAG TATTTCGACT GGGAGTTTAT TTGGTCGTAT 
GATTTTCTGT GGAAGGTTTC TCTCATCACT CTGGTCTCCT GCTTACCATT GTACATAATC 
AAGTTCTTGC GAAAGAAATG TTCGCCGCCC TCTTACTTGA AGCTCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG31729 ( NM_135790.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609634.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG31729), mRNA.

Price & Availability↑

CloneID	OFa14636
Clone ID Related Accession (Same CDS sequence)	NM_135790.4
Accession Version	NM_135790.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135790.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGAGA GCAATGTACC GCTACGAGAT TTCGACGAAG AGAGCGAAAC GGATTCGGAG 
ACCGAATTGC TCATACGCCG CGATGTACCG AGCGGTAAAC AGAACAACAA CAACATTAGG 
GGGCGACGCA AAAGCTCCAT GGGCTACAGC GGCGTCGACG CCGGCGCTGG CAGCTCTGCC 
GCCGGCGGCG GAGGCAGCTT TCTGGGCAAT CTTTTCGGAT CCAACAGTTT TGGCTCTGCC 
GCCGGCCAGA TACGTTATAG AAATGAGCGG AGTCGCGAGC GCGCCAAACA CTCAAACGGT 
AGCTACAGCA GCATTTCGAA ACTGGCGCAA TTAACGCACA CGTCATCGGG CGTCGCGGGG 
GCGGGATCGA TCGGTGGCGG TGGTGGTGGG AGTGGCAGTG CCACCGGCAT TAAAGTAACC 
ACTGGCGATG GCAGTGTAGC GGCATCTGGA GGATCTGCTG CCACGGGGGA TCTCAGCAAT 
GGCGCTACGC ACAGCACAAC CGTCATAGAT CCGGAAAATG GCTCATCCAC GGTCTTCTAC 
CAGCACAATC GGCGTAAAAG CAAGAGTCGC AGCATTGGCA ACGTCTGCAA GTTGTGCCTC 
TGCAGCTGCT GGCGAAAATG GTTCCGGCCA CGGGAGCTGA GAGCCCGCAC CGTAAATCTC 
GGACGGGTCA ATACGGAGAA GTTCCCACCG AACGAGATCC GGAATCAGAA GTACAACTTC 
ATCACCTTCC TGCCGCTGGT GCTGTTCGAG CAGTTTCGCT TCTTCCTCAA CTTGTACTTC 
CTGCTGATGG CGCTGTCGCA GTTCATTCCG GACATCCGGA TCGGTTACCC GTACACCTAC 
TGGGGTCCGC TCGGCTTCGT GCTAATGGTC ACCATTTGCC GAGAGGCGGT CGACGATCTG 
CGTCGGCATC AGCGGGATCA CGAGGTAAAC TCGCAGAAGT ACAAGAGACT GTCGTCCACC 
AACATCAGCG GCTACGAAAT GGTTCCCAGC TCCAAGCTGA AGGTCGGTGA CGTCATAATC 
GTGGAAAAGA ACGAGCGCGT GCCAGCGGAT CTGATCCTGC TGCGCACCAG CGATCGGAGC 
GGATCGGTCT TTGTTCGTAC GGACCAACTT GATGGCGAGA CGGACTGGAA ACCACGTCTC 
GCCGTTCCGT ATACTCAAAA ACTCAGTCGA GATTCGGAGC TACATAGCAT CGATGCCAGT 
TTTTACGTGG AGAAGCCACA GAACGACATT CACAGCTTCA TCGCCACCTT CTGCATGGCA 
GACGGCAGCG AAGACACCGG TCTCAGTGTG GAGAATACCC TGTGGGCCAA CACCGTCGTA 
GCCGCAGGAA CAGCCACAGG AATTGTCATA TATACTGGCT GCGAAACTCG CAGCGTGATG 
AATAACTCAC AGCCAAGGTC CAAAGTTGGA CTGCTAGACA TGGAGATTAA CGGATTGACT 
AAGGTTCTGT TCTGCGCCGT GCTTGGACTG TCGCTAGTCA TGATGATGCT TAAGGGTTTC 
GGCGGTCCTT GGTATAGATA CATGTTCCGC TTCGTTCTTC TCTTCTCCTA CATCATTCCA 
ATCAGTCTGC GTGTCAATTT GGACATGGGA AAGGCGTTCT ACTCATGGCA AATGCAAAAC 
GATTCGAACA TCCAGGGAAC TGTAGTGAGG TCGACCACCA TACCCGAGGA ATTGGGACGA 
ATCTCTTATG TATTGACCGA CAAGACGGGC ACACTCACAC AAAACGAGAT GGTGTTCAAG 
AAGATCCACT TGGGCATTGT ATCGCACGAT GCAGACACTT TCCATCACAT TGGGCAGATG 
ATACAGAAGC TATCGGGAAA CATTCTGCAA CAGCAGCAGG GTAGTTTATC GAGCTCGAGC 
AGCGGCGGAG ACTCGACCAA GCCAATGATG TTTGGCAACA GGATGCGTCG ACCGGAGGGA 
TGGCGGGAAT GGGAGGCGGT GCGAGCACTG GCCCTGTGCC ACAATGTTAC ACCGGTAAGC 
GATGACGAGG ACAACCGATC CGTGTCCACG GCATCGACCG TTACCGGCGG TAACAACTCG 
CCAACCAAAT CTGTGATAAA CATCGAGGCA CCTGGCAGTA CGGACACAGA GCATCAGTAT 
CAGGCTGCGT CTCCAGACGA GATTGCACTA GTAAAGTGGA CTGAGCAGGT GGGACTGACC 
CTGATTGCCA GGGATCTCAA CACGATGACG TTGCAGGTGA AGACCCCCAG CGAAGACAAT 
GACATACTTC TGCACTATCA AATCTTGCAG CTTTTCCCAT TCACTAGCGA GAGCAAGCGC 
ATGGGAATTA TAGTGCGTGA GAGCAAAACT GGTCAGATAA CGTTTTATTT GAAGGGTGCC 
GACGTGGTTA TGTCCAGTAT TGTGCAGTAC AACGACTGGC TAAGCGAGGA GTCAGGAAAC 
ATGGCTAGAG AGGGTCTTCG CACTCTTGTT GTGGCTAAGA AGGTTCTCAC CGAGGAACAG 
TACTCTGACT TTGAGACGCG CTACAATGCT GCTCGACTGA GCATCACCGA TCGTGTCGCC 
AAAGTGGCGG CAGTCACTGA ATCCCTCGAG CGTGAACTGG AACTGCTCTG TTTGACAGGA 
GTGGAGGATC GTCTGCAGGA GAACGTGCGT CCAACGCTGG AGCTACTGCG CAATGCTGGA 
GTTCGCGTTT GGATGCTAAC GGGCGACAAG CTGGAGACTG CTTGCTGTAT TGCCAAGTCC 
TCACAGCTGA TTGGTCGGAA CCAGGGACTC CATGTTTTGC GTTCCGTAAA GACGCGTACC 
GATGCTCACC AGGAGCTGAA CAGCTTCCGG CGTAAGCAGG GTCATGCACT GGTCATCTCA 
GGCGAATCCC TCGAGGTTTG CCTGCAGTAT TATAGACCAG AGTTCCTGGA GCTGGCCACG 
GCCTCGCCGG CCGTCGTTTG CTGTCGCTGC TCGCCCACAC AGAAAGCACA GGTGGTGGCG 
CTCATACAAA AGCACACAGG CAAACGAACC TGTGCCGTGG GTGACGGTGG CAACGATGTC 
AGCATGATTC AGCAGGCGGA CGCTGGAGTT GGAATTGAAG GACGAGAGGG AAGACAAGCC 
TCTCTGGCCG GCGATTTTAG CATTCCGCAG TTTTCGCACA TCGCCAAGCT CCTGCTTATC 
CACGGTCGCA GGAGCTACAA GCGATCGGCG GCACTATCCC AGTTCGTTAT CCACCGCGGC 
TTGATCATAA CCACATTGCA GGCGGTCTTT TCAGCGGTAT TCTACCTCAG CTCGGTTGCT 
TTGTATCAGG GCTTTCTCAT GGTCGGTTAC TCGACGCTGT ACACTATGTT CCCGGTGTTC 
TCATTAGTGC TAGATCAGGA TATCACATCG GAGACGGCCG TCACATACCC AGAGCTTTAC 
AAGGATTTGT CAAAAGGACG CAGTCTAAGC TACAAGACCT TCTTTATATG GGTACTAATC 
AGCATATACC AAGGCGGCGT CATCATGTAC GGAGCATTAA TACTCTTTGT GGATGAGTTT 
ATCCACATTG TGGCGATCAG TTTCTCGGCA TTAATCATGA CTGAGCTCAT CATGGTGGCT 
CTCACCGTGC GTACTTGGCA TAGGTTAATG GTGCTAGCGG AACTATTCAG TTTAGCGCTT 
TATCTCATCT CATTGGCCGT GTTACATGAG TATTTCGACT GGGAGTTTAT TTGGTCGTAT 
GATTTTCTGT GGAAGGTTTC TCTCATCACT CTGGTCTCCT GCTTACCATT GTACATAATC 
AAGTTCTTGC GAAAGAAATG TTCGCCGCCC TCTTACTTGA AGCTCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609634.1
CDS	691..4461
Translation	MAESNVPLRDFDEESETDSETELLIRRDVPSGKQNNNNIRGRRKSSMGYSGVDAGAGSSAAGGGGSFLGNLFGSNSFGSAAGQIRYRNERSRERAKHSNGSYSSISKLAQLTHTSSGVAGAGSIGGGGGGSGSATGIKVTTGDGSVAASGGSAATGDLSNGATHSTTVIDPENGSSTVFYQHNRRKSKSRSIGNVCKLCLCSCWRKWFRPRELRARTVNLGRVNTEKFPPNEIRNQKYNFITFLPLVLFEQFRFFLNLYFLLMALSQFIPDIRIGYPYTYWGPLGFVLMVTICREAVDDLRRHQRDHEVNSQKYKRLSSTNISGYEMVPSSKLKVGDVIIVEKNERVPADLILLRTSDRSGSVFVRTDQLDGETDWKPRLAVPYTQKLSRDSELHSIDASFYVEKPQNDIHSFIATFCMADGSEDTGLSVENTLWANTVVAAGTATGIVIYTGCETRSVMNNSQPRSKVGLLDMEINGLTKVLFCAVLGLSLVMMMLKGFGGPWYRYMFRFVLLFSYIIPISLRVNLDMGKAFYSWQMQNDSNIQGTVVRSTTIPEELGRISYVLTDKTGTLTQNEMVFKKIHLGIVSHDADTFHHIGQMIQKLSGNILQQQQGSLSSSSSGGDSTKPMMFGNRMRRPEGWREWEAVRALALCHNVTPVSDDEDNRSVSTASTVTGGNNSPTKSVINIEAPGSTDTEHQYQAASPDEIALVKWTEQVGLTLIARDLNTMTLQVKTPSEDNDILLHYQILQLFPFTSESKRMGIIVRESKTGQITFYLKGADVVMSSIVQYNDWLSEESGNMAREGLRTLVVAKKVLTEEQYSDFETRYNAARLSITDRVAKVAAVTESLERELELLCLTGVEDRLQENVRPTLELLRNAGVRVWMLTGDKLETACCIAKSSQLIGRNQGLHVLRSVKTRTDAHQELNSFRRKQGHALVISGESLEVCLQYYRPEFLELATASPAVVCCRCSPTQKAQVVALIQKHTGKRTCAVGDGGNDVSMIQQADAGVGIEGREGRQASLAGDFSIPQFSHIAKLLLIHGRRSYKRSAALSQFVIHRGLIITTLQAVFSAVFYLSSVALYQGFLMVGYSTLYTMFPVFSLVLDQDITSETAVTYPELYKDLSKGRSLSYKTFFIWVLISIYQGGVIMYGALILFVDEFIHIVAISFSALIMTELIMVALTVRTWHRLMVLAELFSLALYLISLAVLHEYFDWEFIWSYDFLWKVSLITLVSCLPLYIIKFLRKKCSPPSYLKLS

Target ORF information:

RefSeq Version	NM_135790.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG31729), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135790.4

ORF Insert Sequence:

ATGGCTGAGA GCAATGTACC GCTACGAGAT TTCGACGAAG AGAGCGAAAC GGATTCGGAG 
ACCGAATTGC TCATACGCCG CGATGTACCG AGCGGTAAAC AGAACAACAA CAACATTAGG 
GGGCGACGCA AAAGCTCCAT GGGCTACAGC GGCGTCGACG CCGGCGCTGG CAGCTCTGCC 
GCCGGCGGCG GAGGCAGCTT TCTGGGCAAT CTTTTCGGAT CCAACAGTTT TGGCTCTGCC 
GCCGGCCAGA TACGTTATAG AAATGAGCGG AGTCGCGAGC GCGCCAAACA CTCAAACGGT 
AGCTACAGCA GCATTTCGAA ACTGGCGCAA TTAACGCACA CGTCATCGGG CGTCGCGGGG 
GCGGGATCGA TCGGTGGCGG TGGTGGTGGG AGTGGCAGTG CCACCGGCAT TAAAGTAACC 
ACTGGCGATG GCAGTGTAGC GGCATCTGGA GGATCTGCTG CCACGGGGGA TCTCAGCAAT 
GGCGCTACGC ACAGCACAAC CGTCATAGAT CCGGAAAATG GCTCATCCAC GGTCTTCTAC 
CAGCACAATC GGCGTAAAAG CAAGAGTCGC AGCATTGGCA ACGTCTGCAA GTTGTGCCTC 
TGCAGCTGCT GGCGAAAATG GTTCCGGCCA CGGGAGCTGA GAGCCCGCAC CGTAAATCTC 
GGACGGGTCA ATACGGAGAA GTTCCCACCG AACGAGATCC GGAATCAGAA GTACAACTTC 
ATCACCTTCC TGCCGCTGGT GCTGTTCGAG CAGTTTCGCT TCTTCCTCAA CTTGTACTTC 
CTGCTGATGG CGCTGTCGCA GTTCATTCCG GACATCCGGA TCGGTTACCC GTACACCTAC 
TGGGGTCCGC TCGGCTTCGT GCTAATGGTC ACCATTTGCC GAGAGGCGGT CGACGATCTG 
CGTCGGCATC AGCGGGATCA CGAGGTAAAC TCGCAGAAGT ACAAGAGACT GTCGTCCACC 
AACATCAGCG GCTACGAAAT GGTTCCCAGC TCCAAGCTGA AGGTCGGTGA CGTCATAATC 
GTGGAAAAGA ACGAGCGCGT GCCAGCGGAT CTGATCCTGC TGCGCACCAG CGATCGGAGC 
GGATCGGTCT TTGTTCGTAC GGACCAACTT GATGGCGAGA CGGACTGGAA ACCACGTCTC 
GCCGTTCCGT ATACTCAAAA ACTCAGTCGA GATTCGGAGC TACATAGCAT CGATGCCAGT 
TTTTACGTGG AGAAGCCACA GAACGACATT CACAGCTTCA TCGCCACCTT CTGCATGGCA 
GACGGCAGCG AAGACACCGG TCTCAGTGTG GAGAATACCC TGTGGGCCAA CACCGTCGTA 
GCCGCAGGAA CAGCCACAGG AATTGTCATA TATACTGGCT GCGAAACTCG CAGCGTGATG 
AATAACTCAC AGCCAAGGTC CAAAGTTGGA CTGCTAGACA TGGAGATTAA CGGATTGACT 
AAGGTTCTGT TCTGCGCCGT GCTTGGACTG TCGCTAGTCA TGATGATGCT TAAGGGTTTC 
GGCGGTCCTT GGTATAGATA CATGTTCCGC TTCGTTCTTC TCTTCTCCTA CATCATTCCA 
ATCAGTCTGC GTGTCAATTT GGACATGGGA AAGGCGTTCT ACTCATGGCA AATGCAAAAC 
GATTCGAACA TCCAGGGAAC TGTAGTGAGG TCGACCACCA TACCCGAGGA ATTGGGACGA 
ATCTCTTATG TATTGACCGA CAAGACGGGC ACACTCACAC AAAACGAGAT GGTGTTCAAG 
AAGATCCACT TGGGCATTGT ATCGCACGAT GCAGACACTT TCCATCACAT TGGGCAGATG 
ATACAGAAGC TATCGGGAAA CATTCTGCAA CAGCAGCAGG GTAGTTTATC GAGCTCGAGC 
AGCGGCGGAG ACTCGACCAA GCCAATGATG TTTGGCAACA GGATGCGTCG ACCGGAGGGA 
TGGCGGGAAT GGGAGGCGGT GCGAGCACTG GCCCTGTGCC ACAATGTTAC ACCGGTAAGC 
GATGACGAGG ACAACCGATC CGTGTCCACG GCATCGACCG TTACCGGCGG TAACAACTCG 
CCAACCAAAT CTGTGATAAA CATCGAGGCA CCTGGCAGTA CGGACACAGA GCATCAGTAT 
CAGGCTGCGT CTCCAGACGA GATTGCACTA GTAAAGTGGA CTGAGCAGGT GGGACTGACC 
CTGATTGCCA GGGATCTCAA CACGATGACG TTGCAGGTGA AGACCCCCAG CGAAGACAAT 
GACATACTTC TGCACTATCA AATCTTGCAG CTTTTCCCAT TCACTAGCGA GAGCAAGCGC 
ATGGGAATTA TAGTGCGTGA GAGCAAAACT GGTCAGATAA CGTTTTATTT GAAGGGTGCC 
GACGTGGTTA TGTCCAGTAT TGTGCAGTAC AACGACTGGC TAAGCGAGGA GTCAGGAAAC 
ATGGCTAGAG AGGGTCTTCG CACTCTTGTT GTGGCTAAGA AGGTTCTCAC CGAGGAACAG 
TACTCTGACT TTGAGACGCG CTACAATGCT GCTCGACTGA GCATCACCGA TCGTGTCGCC 
AAAGTGGCGG CAGTCACTGA ATCCCTCGAG CGTGAACTGG AACTGCTCTG TTTGACAGGA 
GTGGAGGATC GTCTGCAGGA GAACGTGCGT CCAACGCTGG AGCTACTGCG CAATGCTGGA 
GTTCGCGTTT GGATGCTAAC GGGCGACAAG CTGGAGACTG CTTGCTGTAT TGCCAAGTCC 
TCACAGCTGA TTGGTCGGAA CCAGGGACTC CATGTTTTGC GTTCCGTAAA GACGCGTACC 
GATGCTCACC AGGAGCTGAA CAGCTTCCGG CGTAAGCAGG GTCATGCACT GGTCATCTCA 
GGCGAATCCC TCGAGGTTTG CCTGCAGTAT TATAGACCAG AGTTCCTGGA GCTGGCCACG 
GCCTCGCCGG CCGTCGTTTG CTGTCGCTGC TCGCCCACAC AGAAAGCACA GGTGGTGGCG 
CTCATACAAA AGCACACAGG CAAACGAACC TGTGCCGTGG GTGACGGTGG CAACGATGTC 
AGCATGATTC AGCAGGCGGA CGCTGGAGTT GGAATTGAAG GACGAGAGGG AAGACAAGCC 
TCTCTGGCCG GCGATTTTAG CATTCCGCAG TTTTCGCACA TCGCCAAGCT CCTGCTTATC 
CACGGTCGCA GGAGCTACAA GCGATCGGCG GCACTATCCC AGTTCGTTAT CCACCGCGGC 
TTGATCATAA CCACATTGCA GGCGGTCTTT TCAGCGGTAT TCTACCTCAG CTCGGTTGCT 
TTGTATCAGG GCTTTCTCAT GGTCGGTTAC TCGACGCTGT ACACTATGTT CCCGGTGTTC 
TCATTAGTGC TAGATCAGGA TATCACATCG GAGACGGCCG TCACATACCC AGAGCTTTAC 
AAGGATTTGT CAAAAGGACG CAGTCTAAGC TACAAGACCT TCTTTATATG GGTACTAATC 
AGCATATACC AAGGCGGCGT CATCATGTAC GGAGCATTAA TACTCTTTGT GGATGAGTTT 
ATCCACATTG TGGCGATCAG TTTCTCGGCA TTAATCATGA CTGAGCTCAT CATGGTGGCT 
CTCACCGTGC GTACTTGGCA TAGGTTAATG GTGCTAGCGG AACTATTCAG TTTAGCGCTT 
TATCTCATCT CATTGGCCGT GTTACATGAG TATTTCGACT GGGAGTTTAT TTGGTCGTAT 
GATTTTCTGT GGAAGGTTTC TCTCATCACT CTGGTCTCCT GCTTACCATT GTACATAATC 
AAGTTCTTGC GAAAGAAATG TTCGCCGCCC TCTTACTTGA AGCTCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG31729 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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