Ttc30 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ttc30
Entrez Gene ID	34702
Full Name	CG5142 gene product from transcript CG5142-RD
Synonyms	Dmel\CG5142,TTC30
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34A5-34A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103685.3	NP_001097155.2	tetratricopeptide repeat domain 30, isoform C
NM_001273501.1	NP_001260430.1	tetratricopeptide repeat domain 30, isoform D

Pathways from BioSystems

Homologies

	TTC30B	XP_001096859.1
Canis lupus familiaris (dog)	TTC30B	XP_852740.2
Homo sapiens (human)	TTC30B	NP_689730.2
Bos taurus (cattle)	TTC30B	NP_001092561.1
Mus musculus (house mouse)	Ttc30b	NP_082511.1
Rattus norvegicus (Norway rat)	Ttc30b	NP_001121079.1
Danio rerio (zebrafish)	flr	NP_001098119.2
Bos taurus (cattle)	TTC30A	NP_001075185.1
Pan troglodytes (chimpanzee)	TTC30B	XP_001154320.2
Canis lupus familiaris (dog)	TTC30A	XP_852753.2
Gallus gallus (chicken)	TTC30A	XP_426574.3
Drosophila melanogaster (fruit fly)	CG5142	NP_001097155.2
Anopheles gambiae (African malaria mosquito)	TTC30_ANOGA	XP_312036.2
Xenopus tropicalis (tropical clawed frog)	ttc30a	NP_001096303.1
Caenorhabditis elegans (roundworm)	dyf-1	NP_491494.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ttc30 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ttc30 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14593	NM_001273501.1 Latest version!	Drosophila melanogaster tetratricopeptide repeat domain 30, transcript variant D (Ttc30), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14593	NM_001103685.3 Latest version!	Drosophila melanogaster tetratricopeptide repeat domain 30, transcript variant C (Ttc30), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ttc30 ( NM_001103685.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097155.2 Drosophila melanogaster tetratricopeptide repeat domain 30, transcript variant C (Ttc30), mRNA.

Price & Availability↑

CloneID	OFa14593
Clone ID Related Accession (Same CDS sequence)	NM_001103685.3 , NM_001273501.1
Accession Version	NM_001103685.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tetratricopeptide repeat domain 30, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_001103685.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCATC AGGGAATTAT CCTGCGTGAG GGGCATGTGA CGAGGACAAT ATATAATCTG 
ATCAAGGACA AGCGCTACGA GGATGTGATC GAGTGCATAA CCAGTTTTGG TGAGGCGGCC 
AACACGAGGG CGGGTCTATC CACCCTGGGA CATTGTTACT ACCACGCCCA GAAATACGAG 
GAGGCGGCCA CCTGCTACGA GCAATTGTGC CAACTGGCGC CCAAGGAAGC CAAGTACAGA 
TTCTACTATG CCCAGTCACT TTATCAGGCT GGCATCTTTG CTGATGCTCT GCGGGTGCTC 
AAGCAAATGG GTGACCAGGA GGATGAGCTC CGGGAGCAGT GTTTGCAGCT GCAGAGTGCG 
ATCCTCTACT CCAGCGAAGA TTTCGCGGGA GCACAGAGCC TGCTGAATCA GCGGGCGGGA 
GGCACTGCAG ATACCCTCAA CGATGAGGGA TGCTTGCTGT TCCAGGCTGA TCAGCACGAG 
GCGGCAGTGC AGCGATTTCA GGCGGCGCTT CAAGTGGGTG GCTTTAACCC ATTAGTGGCC 
TACAATGTGG CGCTGGCACA TTTTCAAAAG AAACAGCGAG CCCAGGCATT GGATTACACA 
TCCGAGATTG TAGAGCGAGG AATGCGCAAT CATCCAGAGC TTGGAATAGG CGCCCAGATG 
GATATTCCCG ATGGCGGTGC CAGGAGCGTG GGCAATCCCA TCACCATGGC CATTTCGGGC 
ATAACTCAGG CACTTAATCT CAAGGCAGCC ATTGAATTTC AAGATGGCAA TGAGGAAGCA 
GCCAGGGACG CCCTCCTGGA CCTTCCTCCA CGAGCCGAAA GCGAACTAGA TCCGGTCACA 
TTGCACAACA TGGCCTTAAC CGATGTCGAA GGTCCTGTGG CGGGTCTGAG GAAGCTGGCG 
TTTCTCCTGG AACTGGGTGC ACCCTCCTGC CCCAAGGAGA CGTTCGCCAA CATTCTGCTC 
ATCTGCTGCA AGAACGAACT TTACGAAACT GCTGCGGATA TCCTGGCGGA GCACACGGAC 
CTCACCTACA AGTATCTTTC GCAATATCTC TATGAGTTAT TGGACTCCCT AATAACCGCA 
CAAACAAGTG CCGAGTTGGC CGAGAAGAAA CTAGGTACTC TGGCCTCCAG TCTTGCTGGT 
AAACTGCGCA GCTTGGCCGC AAAAGTGCAG GAGGTCAGGG CCACCAATGA GCAACAGGCA 
CTCAGGGATG CCCTCAAGGA CTACGAACAG GCACTGGAAC TTTACTTGCC TGTGGTGATG 
GCTCGAGCCT GGATCTCTTG GCGGGATGAT GACTTCGTCG GAGCTGAAAG GGAATTCCAT 
GCCAGTGCCG AGTTCTGTTC GGAGAACTCC ATTTGGCGAT TGAATGCAGG ACATGTGCTC 
TTCATGCAGG GCGACAAATA CAACGAAGCG GCTGCGTTTT ATGAACCCAT CGTGCGCCAG 
CATTCGGATG ATATCATGTC CGTTTCTGCA GCGGTGCTGG CCAACCTGTG CGTCTCCTAC 
ATCATGACAT TCCAGAACGA GGAGGCCGAG GAGCTGATGC GTAAAGTGGA AAAGGCCGAG 
GAGATGAAGG GCAATCTGGG CAAGCAATAC CACCACCTGT GCATTGTCAA TCTGGTGGTG 
GGCACACTGT ACTGCGCCAA ATCCAACTAT GAGTTCGGCC TATCCCGCAT CGCCCATGCC 
CTGGAGAGTG GGTCTGGGAA CCGGCTGTAT GCCGACACCT GGCTGCATGT GAAGCACTGT 
ATCCTGGGGC TTCTCACCGG CATGGCCAAG CAGAACATTA TCCTGCCCTA TGCCACCGTG 
CAGGAGGTCC TCAACTTTCT GCGCTTTTGC GAGTCCTACG GCCTCTTTAC GCCGGCCAAC 
ATTTTTTCGG CCACCGAACA AGTGCCGGAG GAGCCACTTA CCATTGGCCT GGAGGCCAGG 
AAGTTGAGGC TCCTATTGAT TAAGCTGAGC GAATACGATA ACTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097155.2
CDS	15..1982
Translation	MLHQGIILREGHVTRTIYNLIKDKRYEDVIECITSFGEAANTRAGLSTLGHCYYHAQKYEEAATCYEQLCQLAPKEAKYRFYYAQSLYQAGIFADALRVLKQMGDQEDELREQCLQLQSAILYSSEDFAGAQSLLNQRAGGTADTLNDEGCLLFQADQHEAAVQRFQAALQVGGFNPLVAYNVALAHFQKKQRAQALDYTSEIVERGMRNHPELGIGAQMDIPDGGARSVGNPITMAISGITQALNLKAAIEFQDGNEEAARDALLDLPPRAESELDPVTLHNMALTDVEGPVAGLRKLAFLLELGAPSCPKETFANILLICCKNELYETAADILAEHTDLTYKYLSQYLYELLDSLITAQTSAELAEKKLGTLASSLAGKLRSLAAKVQEVRATNEQQALRDALKDYEQALELYLPVVMARAWISWRDDDFVGAEREFHASAEFCSENSIWRLNAGHVLFMQGDKYNEAAAFYEPIVRQHSDDIMSVSAAVLANLCVSYIMTFQNEEAEELMRKVEKAEEMKGNLGKQYHHLCIVNLVVGTLYCAKSNYEFGLSRIAHALESGSGNRLYADTWLHVKHCILGLLTGMAKQNIILPYATVQEVLNFLRFCESYGLFTPANIFSATEQVPEEPLTIGLEARKLRLLLIKLSEYDNF

Target ORF information:

RefSeq Version	NM_001103685.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tetratricopeptide repeat domain 30, transcript variant C (Ttc30), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103685.3

ORF Insert Sequence:

ATGTTGCATC AGGGAATTAT CCTGCGTGAG GGGCATGTGA CGAGGACAAT ATATAATCTG 
ATCAAGGACA AGCGCTACGA GGATGTGATC GAGTGCATAA CCAGTTTTGG TGAGGCGGCC 
AACACGAGGG CGGGTCTATC CACCCTGGGA CATTGTTACT ACCACGCCCA GAAATACGAG 
GAGGCGGCCA CCTGCTACGA GCAATTGTGC CAACTGGCGC CCAAGGAAGC CAAGTACAGA 
TTCTACTATG CCCAGTCACT TTATCAGGCT GGCATCTTTG CTGATGCTCT GCGGGTGCTC 
AAGCAAATGG GTGACCAGGA GGATGAGCTC CGGGAGCAGT GTTTGCAGCT GCAGAGTGCG 
ATCCTCTACT CCAGCGAAGA TTTCGCGGGA GCACAGAGCC TGCTGAATCA GCGGGCGGGA 
GGCACTGCAG ATACCCTCAA CGATGAGGGA TGCTTGCTGT TCCAGGCTGA TCAGCACGAG 
GCGGCAGTGC AGCGATTTCA GGCGGCGCTT CAAGTGGGTG GCTTTAACCC ATTAGTGGCC 
TACAATGTGG CGCTGGCACA TTTTCAAAAG AAACAGCGAG CCCAGGCATT GGATTACACA 
TCCGAGATTG TAGAGCGAGG AATGCGCAAT CATCCAGAGC TTGGAATAGG CGCCCAGATG 
GATATTCCCG ATGGCGGTGC CAGGAGCGTG GGCAATCCCA TCACCATGGC CATTTCGGGC 
ATAACTCAGG CACTTAATCT CAAGGCAGCC ATTGAATTTC AAGATGGCAA TGAGGAAGCA 
GCCAGGGACG CCCTCCTGGA CCTTCCTCCA CGAGCCGAAA GCGAACTAGA TCCGGTCACA 
TTGCACAACA TGGCCTTAAC CGATGTCGAA GGTCCTGTGG CGGGTCTGAG GAAGCTGGCG 
TTTCTCCTGG AACTGGGTGC ACCCTCCTGC CCCAAGGAGA CGTTCGCCAA CATTCTGCTC 
ATCTGCTGCA AGAACGAACT TTACGAAACT GCTGCGGATA TCCTGGCGGA GCACACGGAC 
CTCACCTACA AGTATCTTTC GCAATATCTC TATGAGTTAT TGGACTCCCT AATAACCGCA 
CAAACAAGTG CCGAGTTGGC CGAGAAGAAA CTAGGTACTC TGGCCTCCAG TCTTGCTGGT 
AAACTGCGCA GCTTGGCCGC AAAAGTGCAG GAGGTCAGGG CCACCAATGA GCAACAGGCA 
CTCAGGGATG CCCTCAAGGA CTACGAACAG GCACTGGAAC TTTACTTGCC TGTGGTGATG 
GCTCGAGCCT GGATCTCTTG GCGGGATGAT GACTTCGTCG GAGCTGAAAG GGAATTCCAT 
GCCAGTGCCG AGTTCTGTTC GGAGAACTCC ATTTGGCGAT TGAATGCAGG ACATGTGCTC 
TTCATGCAGG GCGACAAATA CAACGAAGCG GCTGCGTTTT ATGAACCCAT CGTGCGCCAG 
CATTCGGATG ATATCATGTC CGTTTCTGCA GCGGTGCTGG CCAACCTGTG CGTCTCCTAC 
ATCATGACAT TCCAGAACGA GGAGGCCGAG GAGCTGATGC GTAAAGTGGA AAAGGCCGAG 
GAGATGAAGG GCAATCTGGG CAAGCAATAC CACCACCTGT GCATTGTCAA TCTGGTGGTG 
GGCACACTGT ACTGCGCCAA ATCCAACTAT GAGTTCGGCC TATCCCGCAT CGCCCATGCC 
CTGGAGAGTG GGTCTGGGAA CCGGCTGTAT GCCGACACCT GGCTGCATGT GAAGCACTGT 
ATCCTGGGGC TTCTCACCGG CATGGCCAAG CAGAACATTA TCCTGCCCTA TGCCACCGTG 
CAGGAGGTCC TCAACTTTCT GCGCTTTTGC GAGTCCTACG GCCTCTTTAC GCCGGCCAAC 
ATTTTTTCGG CCACCGAACA AGTGCCGGAG GAGCCACTTA CCATTGGCCT GGAGGCCAGG 
AAGTTGAGGC TCCTATTGAT TAAGCTGAGC GAATACGATA ACTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ttc30 ( NM_001273501.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260430.1 Drosophila melanogaster tetratricopeptide repeat domain 30, transcript variant D (Ttc30), mRNA.

Price & Availability↑

CloneID	OFa14593
Clone ID Related Accession (Same CDS sequence)	NM_001103685.3 , NM_001273501.1
Accession Version	NM_001273501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tetratricopeptide repeat domain 30, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCATC AGGGAATTAT CCTGCGTGAG GGGCATGTGA CGAGGACAAT ATATAATCTG 
ATCAAGGACA AGCGCTACGA GGATGTGATC GAGTGCATAA CCAGTTTTGG TGAGGCGGCC 
AACACGAGGG CGGGTCTATC CACCCTGGGA CATTGTTACT ACCACGCCCA GAAATACGAG 
GAGGCGGCCA CCTGCTACGA GCAATTGTGC CAACTGGCGC CCAAGGAAGC CAAGTACAGA 
TTCTACTATG CCCAGTCACT TTATCAGGCT GGCATCTTTG CTGATGCTCT GCGGGTGCTC 
AAGCAAATGG GTGACCAGGA GGATGAGCTC CGGGAGCAGT GTTTGCAGCT GCAGAGTGCG 
ATCCTCTACT CCAGCGAAGA TTTCGCGGGA GCACAGAGCC TGCTGAATCA GCGGGCGGGA 
GGCACTGCAG ATACCCTCAA CGATGAGGGA TGCTTGCTGT TCCAGGCTGA TCAGCACGAG 
GCGGCAGTGC AGCGATTTCA GGCGGCGCTT CAAGTGGGTG GCTTTAACCC ATTAGTGGCC 
TACAATGTGG CGCTGGCACA TTTTCAAAAG AAACAGCGAG CCCAGGCATT GGATTACACA 
TCCGAGATTG TAGAGCGAGG AATGCGCAAT CATCCAGAGC TTGGAATAGG CGCCCAGATG 
GATATTCCCG ATGGCGGTGC CAGGAGCGTG GGCAATCCCA TCACCATGGC CATTTCGGGC 
ATAACTCAGG CACTTAATCT CAAGGCAGCC ATTGAATTTC AAGATGGCAA TGAGGAAGCA 
GCCAGGGACG CCCTCCTGGA CCTTCCTCCA CGAGCCGAAA GCGAACTAGA TCCGGTCACA 
TTGCACAACA TGGCCTTAAC CGATGTCGAA GGTCCTGTGG CGGGTCTGAG GAAGCTGGCG 
TTTCTCCTGG AACTGGGTGC ACCCTCCTGC CCCAAGGAGA CGTTCGCCAA CATTCTGCTC 
ATCTGCTGCA AGAACGAACT TTACGAAACT GCTGCGGATA TCCTGGCGGA GCACACGGAC 
CTCACCTACA AGTATCTTTC GCAATATCTC TATGAGTTAT TGGACTCCCT AATAACCGCA 
CAAACAAGTG CCGAGTTGGC CGAGAAGAAA CTAGGTACTC TGGCCTCCAG TCTTGCTGGT 
AAACTGCGCA GCTTGGCCGC AAAAGTGCAG GAGGTCAGGG CCACCAATGA GCAACAGGCA 
CTCAGGGATG CCCTCAAGGA CTACGAACAG GCACTGGAAC TTTACTTGCC TGTGGTGATG 
GCTCGAGCCT GGATCTCTTG GCGGGATGAT GACTTCGTCG GAGCTGAAAG GGAATTCCAT 
GCCAGTGCCG AGTTCTGTTC GGAGAACTCC ATTTGGCGAT TGAATGCAGG ACATGTGCTC 
TTCATGCAGG GCGACAAATA CAACGAAGCG GCTGCGTTTT ATGAACCCAT CGTGCGCCAG 
CATTCGGATG ATATCATGTC CGTTTCTGCA GCGGTGCTGG CCAACCTGTG CGTCTCCTAC 
ATCATGACAT TCCAGAACGA GGAGGCCGAG GAGCTGATGC GTAAAGTGGA AAAGGCCGAG 
GAGATGAAGG GCAATCTGGG CAAGCAATAC CACCACCTGT GCATTGTCAA TCTGGTGGTG 
GGCACACTGT ACTGCGCCAA ATCCAACTAT GAGTTCGGCC TATCCCGCAT CGCCCATGCC 
CTGGAGAGTG GGTCTGGGAA CCGGCTGTAT GCCGACACCT GGCTGCATGT GAAGCACTGT 
ATCCTGGGGC TTCTCACCGG CATGGCCAAG CAGAACATTA TCCTGCCCTA TGCCACCGTG 
CAGGAGGTCC TCAACTTTCT GCGCTTTTGC GAGTCCTACG GCCTCTTTAC GCCGGCCAAC 
ATTTTTTCGG CCACCGAACA AGTGCCGGAG GAGCCACTTA CCATTGGCCT GGAGGCCAGG 
AAGTTGAGGC TCCTATTGAT TAAGCTGAGC GAATACGATA ACTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260430.1
CDS	79..2046
Translation	MLHQGIILREGHVTRTIYNLIKDKRYEDVIECITSFGEAANTRAGLSTLGHCYYHAQKYEEAATCYEQLCQLAPKEAKYRFYYAQSLYQAGIFADALRVLKQMGDQEDELREQCLQLQSAILYSSEDFAGAQSLLNQRAGGTADTLNDEGCLLFQADQHEAAVQRFQAALQVGGFNPLVAYNVALAHFQKKQRAQALDYTSEIVERGMRNHPELGIGAQMDIPDGGARSVGNPITMAISGITQALNLKAAIEFQDGNEEAARDALLDLPPRAESELDPVTLHNMALTDVEGPVAGLRKLAFLLELGAPSCPKETFANILLICCKNELYETAADILAEHTDLTYKYLSQYLYELLDSLITAQTSAELAEKKLGTLASSLAGKLRSLAAKVQEVRATNEQQALRDALKDYEQALELYLPVVMARAWISWRDDDFVGAEREFHASAEFCSENSIWRLNAGHVLFMQGDKYNEAAAFYEPIVRQHSDDIMSVSAAVLANLCVSYIMTFQNEEAEELMRKVEKAEEMKGNLGKQYHHLCIVNLVVGTLYCAKSNYEFGLSRIAHALESGSGNRLYADTWLHVKHCILGLLTGMAKQNIILPYATVQEVLNFLRFCESYGLFTPANIFSATEQVPEEPLTIGLEARKLRLLLIKLSEYDNF

Target ORF information:

RefSeq Version	NM_001273501.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tetratricopeptide repeat domain 30, transcript variant D (Ttc30), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273501.1

ORF Insert Sequence:

ATGTTGCATC AGGGAATTAT CCTGCGTGAG GGGCATGTGA CGAGGACAAT ATATAATCTG 
ATCAAGGACA AGCGCTACGA GGATGTGATC GAGTGCATAA CCAGTTTTGG TGAGGCGGCC 
AACACGAGGG CGGGTCTATC CACCCTGGGA CATTGTTACT ACCACGCCCA GAAATACGAG 
GAGGCGGCCA CCTGCTACGA GCAATTGTGC CAACTGGCGC CCAAGGAAGC CAAGTACAGA 
TTCTACTATG CCCAGTCACT TTATCAGGCT GGCATCTTTG CTGATGCTCT GCGGGTGCTC 
AAGCAAATGG GTGACCAGGA GGATGAGCTC CGGGAGCAGT GTTTGCAGCT GCAGAGTGCG 
ATCCTCTACT CCAGCGAAGA TTTCGCGGGA GCACAGAGCC TGCTGAATCA GCGGGCGGGA 
GGCACTGCAG ATACCCTCAA CGATGAGGGA TGCTTGCTGT TCCAGGCTGA TCAGCACGAG 
GCGGCAGTGC AGCGATTTCA GGCGGCGCTT CAAGTGGGTG GCTTTAACCC ATTAGTGGCC 
TACAATGTGG CGCTGGCACA TTTTCAAAAG AAACAGCGAG CCCAGGCATT GGATTACACA 
TCCGAGATTG TAGAGCGAGG AATGCGCAAT CATCCAGAGC TTGGAATAGG CGCCCAGATG 
GATATTCCCG ATGGCGGTGC CAGGAGCGTG GGCAATCCCA TCACCATGGC CATTTCGGGC 
ATAACTCAGG CACTTAATCT CAAGGCAGCC ATTGAATTTC AAGATGGCAA TGAGGAAGCA 
GCCAGGGACG CCCTCCTGGA CCTTCCTCCA CGAGCCGAAA GCGAACTAGA TCCGGTCACA 
TTGCACAACA TGGCCTTAAC CGATGTCGAA GGTCCTGTGG CGGGTCTGAG GAAGCTGGCG 
TTTCTCCTGG AACTGGGTGC ACCCTCCTGC CCCAAGGAGA CGTTCGCCAA CATTCTGCTC 
ATCTGCTGCA AGAACGAACT TTACGAAACT GCTGCGGATA TCCTGGCGGA GCACACGGAC 
CTCACCTACA AGTATCTTTC GCAATATCTC TATGAGTTAT TGGACTCCCT AATAACCGCA 
CAAACAAGTG CCGAGTTGGC CGAGAAGAAA CTAGGTACTC TGGCCTCCAG TCTTGCTGGT 
AAACTGCGCA GCTTGGCCGC AAAAGTGCAG GAGGTCAGGG CCACCAATGA GCAACAGGCA 
CTCAGGGATG CCCTCAAGGA CTACGAACAG GCACTGGAAC TTTACTTGCC TGTGGTGATG 
GCTCGAGCCT GGATCTCTTG GCGGGATGAT GACTTCGTCG GAGCTGAAAG GGAATTCCAT 
GCCAGTGCCG AGTTCTGTTC GGAGAACTCC ATTTGGCGAT TGAATGCAGG ACATGTGCTC 
TTCATGCAGG GCGACAAATA CAACGAAGCG GCTGCGTTTT ATGAACCCAT CGTGCGCCAG 
CATTCGGATG ATATCATGTC CGTTTCTGCA GCGGTGCTGG CCAACCTGTG CGTCTCCTAC 
ATCATGACAT TCCAGAACGA GGAGGCCGAG GAGCTGATGC GTAAAGTGGA AAAGGCCGAG 
GAGATGAAGG GCAATCTGGG CAAGCAATAC CACCACCTGT GCATTGTCAA TCTGGTGGTG 
GGCACACTGT ACTGCGCCAA ATCCAACTAT GAGTTCGGCC TATCCCGCAT CGCCCATGCC 
CTGGAGAGTG GGTCTGGGAA CCGGCTGTAT GCCGACACCT GGCTGCATGT GAAGCACTGT 
ATCCTGGGGC TTCTCACCGG CATGGCCAAG CAGAACATTA TCCTGCCCTA TGCCACCGTG 
CAGGAGGTCC TCAACTTTCT GCGCTTTTGC GAGTCCTACG GCCTCTTTAC GCCGGCCAAC 
ATTTTTTCGG CCACCGAACA AGTGCCGGAG GAGCCACTTA CCATTGGCCT GGAGGCCAGG 
AAGTTGAGGC TCCTATTGAT TAAGCTGAGC GAATACGATA ACTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ttc30 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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